FB2025_02 , released April 17, 2025
Gene: Dmel\l(2)efl
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General Information
Symbol
Dmel\l(2)efl
Species
D. melanogaster
Name
lethal (2) essential for life
Annotation Symbol
CG4533
Feature Type
protein_coding_gene
FlyBase ID
FBgn0011296
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Summary
Vital role in embryonic development. (UniProt, P82147)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

CryAB, dCryAB

Key Links
Genomic Location
Cytogenetic map
59F6-59F6
Sequence location
Recombination map
2-104
RefSeq locus
NT_033778 REGION:23684651..23685543
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (10 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables unfolded protein binding
inferred from biological aspect of ancestor with PANTHER:PTN000897708
(GO Reference Genome Project, 2011-)
inferred from sequence model
(Li et al., 2009)
Biological Process (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in protein refolding
inferred from direct assay
(Vos et al., 2016)
involved_in regulation of translational initiation
inferred from mutant phenotype
(Carra et al., 2010)
involved_in response to heat
inferred from direct assay
(Vos et al., 2016)
involved_in sarcomere organization
inferred from mutant phenotype
(Wójtowicz et al., 2015)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in chaperone-mediated protein folding
inferred from sequence model
(Li et al., 2009)
involved_in protein refolding
inferred from biological aspect of ancestor with PANTHER:PTN000897708
(GO Reference Genome Project, 2011-)
involved_in response to heat
inferred from biological aspect of ancestor with PANTHER:PTN000897708
(GO Reference Genome Project, 2011-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in perinuclear region of cytoplasm
inferred from direct assay
(Wójtowicz et al., 2015)
located_in Z disc
inferred from direct assay
(Wójtowicz et al., 2015)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000897708
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000897708
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the small heat shock protein (HSP20) family. (P82147)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
SMALL HEAT SHOCK PROTEINS -
The small Heat Shock Protein (sHSP) family is characterized by the presence of an α-crystallin domain. sHSPs play a crucial role in protein folding. Most sHSPs have the ability to prevent protein aggregation and to maintain substrate proteins in a refoldable state. (Adapted from FBrf0228040 and FBrf0231212).
Protein Function (UniProtKB)
Vital role in embryonic development.
(UniProt, P82147)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\l(2)efl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P82147)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
l(2)efl-RA
FBtr0072100
NM_079103
738
187
l(2)efl-RC
FBtr0336685
NM_001274227
741
187
Additional Transcript Data and Comments
Reported size (kB)

1.4 (northern blot)

(Kurzik-Dumke and Lohmann, 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
l(2)efl-PA
FBpp0072009
21.3
187
6.13
P82147
NP_523827
AAF47041
l(2)efl-PC
FBpp0307666
21.3
187
6.13
P82147
NP_001261156
AGB93686
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

187 aa isoforms: l(2)efl-PA, l(2)efl-PC
Additional Polypeptide Data and Comments
Reported size (kDa)

187 (aa)

(Kurzik-Dumke and Lohmann, 1995)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\l(2)efl using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.42

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 16
larval muscle system
(Fisher et al., 2012)
larval somatic muscle cell
(Fisher et al., 2012)
larval visceral muscle cell
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Kurzik-Dumke and Lohmann, 1995)
embryonic stage -- adult stage
(Kurzik-Dumke and Lohmann, 1995)
Additional Descriptive Data

The l(2)efl transcript is expressed throughout development. Expression is weakest during the first 15 hours of embryogenesis, and peaks at third larval instar. Embryo whole-mount in situ hybridization experiments show that expression is ubiquitous up to stage 16 of embryogenesis.

(Kurzik-Dumke and Lohmann, 1995)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval hypodermal muscle cell

Comment: in Z disc

(Wójtowicz et al., 2015)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data

l(2)efl is abundantly expressed in larval body wall muscles. Within an individual muscle, it accumulates in the perinuclear area and displays a striated sarcomeric pattern at the level of Z-lines and M-lines.

(Wójtowicz et al., 2015)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in perinuclear region of cytoplasm
inferred from direct assay
(Wójtowicz et al., 2015)
located_in Z disc
inferred from direct assay
(Wójtowicz et al., 2015)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\l(2)efl in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 19 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of l(2)efl
Transgenic constructs containing regulatory region of l(2)efl
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2R)Exel7180
(Ryder, 2004.4.26)
Inferred from location ( 6 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die before end of larval stage
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal locomotor behavior | third instar larval stage, with Scer\GAL4Mef2.PR
decreased cell number | embryonic stage 15
decreased size | adult stage, with Scer\GAL4en-e16E
increased mortality during development, with Scer\GAL4Mef2.PR
long lived, with Scer\GAL4arm.PS
long lived, with Scer\GAL4elav-C155
visible | adult stage, with Scer\GAL4en-e16E
Phenotype manifest in
Allele
adult heart
adult heart, with Scer\GAL4Hand.PA
embryonic/larval hypodermal muscle cell | third instar larval stage, with Scer\GAL4Mef2.PR
lateral transverse muscle cell | decreased number | embryonic stage 15
lateral transverse muscle cell | embryonic stage 15
mitochondrion | third instar larval stage, with Scer\GAL4Mef2.PR
muscle cell of ventral longitudinal muscle 3 | third instar larval stage, with Scer\GAL4Mef2.PR
muscle cell of ventral longitudinal muscle 4 | third instar larval stage, with Scer\GAL4Mef2.PR
segment border muscle cell | third instar larval stage, with Scer\GAL4Mef2.PR
wing, with Scer\GAL4en-e16E
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (12)
Hsap\CRYAB
12 of 14
Yes
Yes
6  
Hsap\CRYAA2
11 of 14
No
Yes
Hsap\CRYAA
11 of 14
No
Yes
Hsap\HSPB6
8 of 14
No
Yes
Hsap\HSPB1
4 of 14
No
No
3  
Hsap\HSPB2
4 of 14
No
Yes
Hsap\HSPB3
4 of 14
No
Yes
2  
Hsap\HSPB8
4 of 14
No
Yes
7  
Hsap\HSPB7
3 of 14
No
Yes
3  
Hsap\HSPB9
3 of 14
No
Yes
Hsap\HSPB2-C11orf52
1 of 14
No
Yes
Hsap\ODF1
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (10)
Rnor\Cryab
13 of 14
Yes
Yes
Rnor\Cryaa
11 of 14
No
Yes
Rnor\Hspb6
10 of 14
No
Yes
Rnor\Hspb1
4 of 14
No
No
Rnor\Hspb2
4 of 14
No
Yes
Rnor\Hspb3
4 of 14
No
Yes
Rnor\Hspb8
4 of 14
No
Yes
Rnor\Hspb7
3 of 14
No
Yes
Rnor\Hspb9
3 of 14
No
Yes
Rnor\Odf1
1 of 14
No
Yes
Mus musculus (laboratory mouse) (10)
Mmus\Cryab
13 of 14
Yes
Yes
Mmus\Cryaa
11 of 14
No
Yes
Mmus\Hspb6
9 of 14
No
Yes
Mmus\Hspb1
4 of 14
No
No
Mmus\Hspb2
4 of 14
No
Yes
Mmus\Hspb3
4 of 14
No
Yes
Mmus\Hspb7
3 of 14
No
Yes
Mmus\Hspb8
3 of 14
No
Yes
Mmus\Hspb9
2 of 14
No
Yes
Mmus\Odf1
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (15)
Xtro\cryab
10 of 13
Yes
Yes
Xtro\hspb6
9 of 13
No
Yes
Xtro\cryaa
8 of 13
No
Yes
Xtro\hspb2
2 of 13
No
Yes
Xtro\hspb3
2 of 13
No
Yes
Xtro\hspb8
2 of 13
No
Yes
Xtro\LOC100489058
2 of 13
No
No
Xtro\LOC100489207
2 of 13
No
No
Xtro\LOC100491399
2 of 13
No
No
Xtro\cblb
1 of 13
No
No
Xtro\cited1
1 of 13
No
Yes
Xtro\hsp30d
1 of 13
No
No
Xtro\hsp30e
1 of 13
No
Yes
Xtro\hspb1
1 of 13
No
No
Xtro\LOC101732374
1 of 13
No
No
Danio rerio (Zebrafish) (13)
Drer\cryaba
12 of 14
Yes
Yes
Drer\cryaa
11 of 14
No
Yes
Drer\cryabb
11 of 14
No
Yes
Drer\hspb6
6 of 14
No
No
Drer\hspb1
4 of 14
No
No
Drer\hspb3
4 of 14
No
Yes
Drer\hspb11
4 of 14
No
Yes
Drer\hspb15
4 of 14
No
No
Drer\si:dkey-1k23.3
4 of 14
No
No
Drer\hspb2
3 of 14
No
Yes
Drer\hspb8
3 of 14
No
No
Drer\hspb9
3 of 14
No
Yes
Drer\hspb7
2 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (19)
Cele\hsp-17
8 of 14
Yes
Yes
Cele\F08H9.3
7 of 14
No
No
Cele\hsp-12.2
7 of 14
No
Yes
Cele\hsp-12.1
6 of 14
No
Yes
Cele\hsp-12.3
6 of 14
No
Yes
Cele\hsp-12.6
6 of 14
No
Yes
Cele\hsp-16.1
6 of 14
No
No
Cele\hsp-16.2
6 of 14
No
No
Cele\hsp-16.11
6 of 14
No
No
Cele\hsp-16.41
6 of 14
No
No
Cele\hsp-16.48
6 of 14
No
No
Cele\hsp-16.49
6 of 14
No
No
Cele\F08H9.4
5 of 14
No
No
Cele\sip-1
5 of 14
No
No
Cele\Y55F3BR.6
5 of 14
No
Yes
Cele\ZK1128.7
5 of 14
No
Yes
Cele\hsp-43
3 of 14
No
Yes
Cele\hsp-25
2 of 14
No
No
Cele\F58H7.1
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (8)
Agam\AgaP_AGAP007162
11 of 12
Yes
Yes
Agam\AgaP_AGAP005547
7 of 12
No
Yes
Agam\AgaP_AGAP005548
7 of 12
No
Yes
Agam\AgaP_AGAP007161
7 of 12
No
Yes
Agam\AgaP_AGAP007158
6 of 12
No
Yes
Agam\AgaP_AGAP007159
6 of 12
No
Yes
Agam\AgaP_AGAP007160
5 of 12
No
Yes
Agam\AgaP_AGAP000941
2 of 12
No
No
Arabidopsis thaliana (thale-cress) (19)
Atha\AT1G07400
4 of 13
Yes
No
Atha\AT1G52560
4 of 13
Yes
Yes
Atha\AT1G53540
4 of 13
Yes
No
Atha\AT1G59860
4 of 13
Yes
No
Atha\AT2G29500
4 of 13
Yes
No
Atha\AT5G37670
4 of 13
Yes
Yes
Atha\ATHSP22.0
4 of 13
Yes
No
Atha\HSP17.4
4 of 13
Yes
No
Atha\HSP18.2
4 of 13
Yes
No
Atha\HSP21
4 of 13
Yes
No
Atha\AT4G21870
3 of 13
No
No
Atha\HSP17.6A
3 of 13
No
No
Atha\HSP17.6II
3 of 13
No
No
Atha\HSP23.6-MITO
3 of 13
No
No
Atha\AT1G54050
2 of 13
No
No
Atha\AT5G51440
2 of 13
No
No
Atha\ACD32.1
1 of 13
No
Yes
Atha\AT1G76770
1 of 13
No
Yes
Atha\AT2G19310
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\HSP26
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\hsp16
1 of 12
Yes
No
Spom\SPCC338.06c
1 of 12
Yes
Yes
Escherichia coli (enterobacterium) (2)
Ecol\ibpA
3 of 11
Yes
Yes
Ecol\ibpB
3 of 11
Yes
Yes
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:l(2)efl. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (10)
Hsp23
10 of 13
Hsp27
10 of 13
Hsp67Bc
10 of 13
Hsp26
9 of 13
Hsp67Ba
9 of 13
CG4461
8 of 13
CG7409
7 of 13
CG13133
7 of 13
Hsp22
7 of 13
CG14207
6 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
l(2)eflR120G.UASp.Venus
model of  myofibrillar myopathy 2
CEA
(Wójtowicz et al., 2015)
Potential Models Based on Orthology ( 8 )
Modifiers Based on Experimental Evidence ( 2 )
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
CRYAB; crystallin alpha B
12 of 14
123590
 
Hsap\CRYAA2
11 of 14
      CRYAA; crystallin alpha A
      11 of 14
      123580
      HSPB6; heat shock protein family B (small) member 6
      8 of 14
      610695
          HSPB1; heat shock protein family B (small) member 1
          4 of 14
          602195
           
          HSPB2; heat shock protein family B (small) member 2
          4 of 14
          602179
              HSPB3; heat shock protein family B (small) member 3
              4 of 14
              604624
               
              HSPB8; heat shock protein family B (small) member 8
              4 of 14
              608014
               
              HSPB7; heat shock protein family B (small) member 7
              3 of 14
              610692
                   
                  HSPB9; heat shock protein family B (small) member 9
                  3 of 14
                  608344
                      Functional Complementation Data
                      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                      Interactions
                      Summary of Physical Interactions
                      Summary of Genetic Interactions
                      Interaction Browsers
                      Starting gene(s)
                      Interaction type
                      Interacting gene(s)
                      Reference
                      Starting gene(s)
                      Interaction type
                      Interacting gene(s)
                      Reference
                      External Data
                      Linkouts
                      BioGRID - A database of protein and genetic interactions.
                      DroID - A comprehensive database of gene and protein interactions.
                      MIST (protein-protein) - An integrated Molecular Interaction Database
                      Pathways
                      Signaling Pathways (FlyBase)
                      Metabolic Pathways
                      FlyBase
                      External Links
                      External Data
                      Linkouts
                      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                      Class of Gene
                      Genomic Location and Detailed Mapping Data
                      Chromosome (arm)
                      2R
                      Recombination map
                      2-104
                      Cytogenetic map
                      59F6-59F6
                      Sequence location
                      FlyBase Computed Cytological Location
                      Cytogenetic map
                      Evidence for location
                      59F6-59F6
                      Limits computationally determined from genome sequence between P{PZ}apt03041 and P{EP}eglEP938
                      Experimentally Determined Cytological Location
                      Cytogenetic map
                      Notes
                      References
                      59F5-59F5
                      (Kurzik-Dumke, 1999.11.4)
                      59F-59F
                      (determined by in situ hybridisation)
                      (Kurzik-Dumke et al., 1995)
                      59F4-59F5
                      (determined by in situ hybridisation)
                      (Kurzik-Dumke and Lohmann, 1995)
                      Experimentally Determined Recombination Data
                      Location

                      2-104

                      (FlyBase, 2016)

                      2-108.8

                      (Comeron et al., 2012)
                      Left of (cM)
                      Right of (cM)
                      Notes
                      Stocks and Reagents
                      Stocks (10)
                      Genomic Clones (12)
                       

                      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                      cDNA Clones (58)
                       

                      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                      cDNA clones, fully sequenced
                      BDGP DGC clones
                      Other clones
                      Drosophila Genomics Resource Center cDNA clones

                      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                      cDNA Clones, End Sequenced (ESTs)
                      BDGP DGC clones
                      Other clones
                      RNAi and Array Information
                      Linkouts
                      DRSC - Results frm RNAi screens
                      Antibody Information
                      Laboratory Generated Antibodies
                       

                      polyclonal

                      (Zhao et al., 2023, Wójtowicz et al., 2015)
                      Commercially Available Antibodies
                       
                      Cell Line Information
                      Publicly Available Cell Lines
                       
                        Other Stable Cell Lines
                         
                          Other Comments

                          l(2)efl is cloned, gene structure determined and temporal expression pattern studied.

                          (Kurzik-Dumke and Lohmann, 1995)
                          Relationship to Other Genes
                          Source for database merge of
                          Additional comments
                          Nomenclature History
                          Source for database identify of

                          Source for identity of: l(2)efl CG4533

                          (Zhang, 1999.11)
                          Nomenclature comments
                          Etymology
                          Synonyms and Secondary IDs (16)
                          Reported As
                          Symbol Synonym
                          CG4533
                          (National Institute of Genetics Fly Stocks, 2022-, Çiçek et al., 2016, Vos et al., 2016, Shanshan and Laixin, 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Sun et al., 2010, Keleman et al., 2009.8.5, Lebo et al., 2009, Goldman and Arbeitman, 2007, Jensen et al., 2006)
                          CryAB
                          (Zhao et al., 2023, Brooks et al., 2020, Wójtowicz et al., 2015)
                          Cryab
                          (Wang et al., 2011)
                          Dmel19.8
                          (Li et al., 2009)
                          L(2)EFL
                          (Vos et al., 2016)
                          L(2)efl
                          (Känel et al., 2022, Gan et al., 2021, Carra et al., 2010, Jensen et al., 2006)
                          dCRYAB
                          (Pueyo et al., 2023)
                          dCryAB
                          (Bertin et al., 2021, Poovathumkadavil and Jagla, 2020, Jagla et al., 2018)
                          el(2)13-1
                          l(2)13-1
                          l(2)59Fa
                          l(2)Efl
                          (Landis et al., 2004)
                          l(2)efl
                          (Lee and Min, 2024, Li et al., 2024, Meyer et al., 2024, Brener et al., 2023, Lee et al., 2023, Adashev et al., 2022, Känel et al., 2022, Perlegos et al., 2022, Malkeyeva et al., 2020, Rivera et al., 2019, Al-Ramahi et al., 2018, Jagla et al., 2018, Hu et al., 2017.6.13, Jo and Imm, 2017, Transgenic RNAi Project members, 2017-, Dialynas et al., 2015, Spratford and Kumar, 2015, Wójtowicz et al., 2015, Belozerov et al., 2014, Huang et al., 2014, Liu et al., 2014, Laslo et al., 2013, Wang et al., 2013, Awofala et al., 2012, Eleftherianos and Castillo, 2012, Japanese National Institute of Genetics, 2012.5.21, Lee et al., 2012, Boyd et al., 2011, Tower, 2011, Wang et al., 2011, Kong et al., 2010, Sun et al., 2010, Hull-Thompson et al., 2009, Flatt et al., 2008, Morozova et al., 2006, Neal et al., 2006, Rehwinkel et al., 2004)
                          Name Synonyms
                          Lethal (2) essential for life
                          (Manh et al., 2005)
                          l(2)efl
                          (Biteau et al., 2011)
                          lethal (2) essential for life
                          (Boyd et al., 2011, Cammarato et al., 2011, Lebo et al., 2009, McGraw et al., 2008, Goldman and Arbeitman, 2007, Harbison et al., 2005, Landis et al., 2004, Kurzik-Dumke et al., 1995)
                          lethal(2)essential for life
                          (Lai et al., 2007)
                          Secondary FlyBase IDs
                          • FBgn0011779
                          Datasets (0)
                          Study focus (0)
                          Experimental Role
                          Project
                          Project Type
                          Title
                          Study result (0)
                          Result
                          Result Type
                          Title
                          External Crossreferences and Linkouts ( 50 )
                          Sequence Crossreferences
                          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                          Other crossreferences
                          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                          FlyMine - An integrated database for Drosophila genomics
                          InterPro - A database of protein families, domains and functional sites
                          KEGG Genes - Molecular building blocks of life in the genomic space.
                          MARRVEL_MODEL - MARRVEL (model organism gene)
                          Linkouts
                          BioGRID - A database of protein and genetic interactions.
                          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                          DroID - A comprehensive database of gene and protein interactions.
                          DRSC - Results frm RNAi screens
                          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                          Flygut - An atlas of the Drosophila adult midgut
                          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                          MIST (protein-protein) - An integrated Molecular Interaction Database
                          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                          References (98)