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General Information
Symbol
Dmel\Alg3
Species
D. melanogaster
Name
ALG3, alpha-1,3- mannosyltransferase
Annotation Symbol
CG4084
Feature Type
FlyBase ID
FBgn0011297
Gene Model Status
Stock Availability
Enzyme Name (EC)
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase (2.4.1.258)
Gene Summary
Contribute a Gene Snapshot for this gene.
Also Known As

l(2)not, l(2)tid, lethal(2)tumorous imaginal discs, tid, l(2)tud

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
2-104
RefSeq locus
NT_033778 REGION:23705439..23709792
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000291297
(assigned by GO_Central )
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000291297
(assigned by GO_Central )
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000291297
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the glycosyltransferase 58 family. (P82149Q27333)
Protein Signatures (InterPro)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D- GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man- alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))- D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate (2.4.1.258)
Summaries
Gene Group (FlyBase)
DOLICHYL PYROPHOSPHATE MANNOSYLTRANSFERASES -
Dolichyl pyrophosphate mannosyltransferases add mannosyl residues to the 'chitobiose core' in the endoplasmic reticulum membrane during the process of protein N-glycosylation. The chitobiose core is composed of two N-acetylglucosamine (GlcNAc) residues attached to the dolichol pyrophosphate (PP-Dol) lipid carrier ((GlcNAc[[2]]-PP-Dol)). Alg1, Alg2 and Alg11 enzymes act first to add extend this core with 5 mannose residues to form the Man[[5]]GlcNAc[[2]]-PP-Dol intermediate. The Alg3, Alg12 and Alg9 enzymes then further elongate this to form Man[[9]]GlcNAc[[2]]-PP-Dol. Following a final glucosylation step, the finished precursor oligosaccharide (Glc[[3]]Man[[9]]GlcNAc[[2]]-PP-Dol) is transferred to nascent peptides, where it will be further trimmed down and modified to produce the final glycoprotein. (Adapted from FBrf0235108.)
Pathway (FlyBase)
Negative Regulators of TNFα-Eiger Signaling Pathway -
Negative regulators of Tumor Necrosis Factor α (TNFα) signaling pathway down-regulate the activation of the pathway triggered by Eiger (egr).
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Alg3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q27333)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0072095
1704
510
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0072004
58.4
510
9.99
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

510 (aa); 45 (kD observed); 56 (kD predicted)

Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Alg3 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

l(2)not transcript is detected ubiquitously throughout embryonic development. It is detected in all larval tissue except for the brain and salivary glands.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

l(2)not protein is detected ubiquitously throughout embryonic development, but is not detected in larval stages.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Alg3 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Alg3
Transgenic constructs containing regulatory region of Alg3
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene partially duplicated in
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
13 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
13 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
12 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
11 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
9 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
14 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
11 of 12
Yes
Yes
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Interactions Browser
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
Pathways
Signaling Pathways (FlyBase)
Negative Regulators of TNFα-Eiger Signaling Pathway -
Negative regulators of Tumor Necrosis Factor α (TNFα) signaling pathway down-regulate the activation of the pathway triggered by Eiger (egr).
Metabolic Pathways
FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-104
Cytogenetic map
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
59F6-59F6
Limits computationally determined from genome sequence between P{PZ}apt03041 and P{EP}eglEP938
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (13)
Genomic Clones (19)
cDNA Clones (32)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: l(2)not CG4084

Source for identity of: Alg3 l(2)not

Source for database merge of
Additional comments

The mutant phenotype of the "tumorous imaginal discs" (tid) gene was originally described in Kurzik-Dumke et al. 1992 (FBrf0055923), and was subsequently reported to encode a DnaJ-like molecular chap- erone in Kurzik-Dumke et al. 1995 (FBrf0080183). However, the "tid" gene was cloned incorrectly - aberrant cell proliferation in tid mutants arises not from disruptions to the DnaJ homolog (CG5504) but rather to an adjacent gene that encodes the mannosyltransferase Alg3 (CG4084, also called "neighbor of tid", "l(2)not"). To avoid confusion with the previous annotation, the CG4084 gene is referred to as "alg3". FlyBase curator comment: Also to avoid confusion, the "l(2)tid" gene has been renamed back to its CG identifier (CG5504).

Other Comments

In some strains the Alg3, l(2)rot and CG5504 genes are duplicated in tandem (Dp(2;2)KD). This 5327-bp duplication has been found in the Harvich and "a px o" strains, but not in Oregon-R or a "b If" strain.

The l(2)rot open reading frame is located on the coding DNA strand localised in the Alg3 intron.

CG5504, Alg3 and l(2)rot are nested genes with complex genetic and molecular interactions. Alg3 and l(2)rot may act upstream of CG5504, and be involved in its transcriptional regulation.

Mutants display an imaginal disc neoplastic phenotype.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 56 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
Other crossreferences
AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
InterPro - A database of protein families, domains and functional sites
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL - MARRVEL (model organism gene)
modMine - A data warehouse for the modENCODE project
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (26)
Reported As
Name Synonyms
ALG3, alpha-1,3- mannosyltransferase
Asparagine-Linked Glycosylation-3
l(2)tumorous disc
l(2)tumorous imaginal discs
lethal (2) neighbor of tid
lethal(2) neighbour of 1(2)tid
lethal(2) tumorous imaginal discs
lethal(2)neighbour of tid
tumorous imaginal disc
α1,3-mannosyltransferase
Secondary FlyBase IDs
    Datasets (8)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    Study result (8)
    Result
    Result Type
    Title
    Clustering analysis of hemocytes from non-infested third instar larvae
    Clustering analysis of hemocytes from wasp-infested third instar larvae
    Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
    Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
    Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
    Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
    Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
    Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
    References (76)