Gene Dmel\Rsf1

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General Information

Symbol

Dmel\Rsf1

Species

D. melanogaster

Name

Repressor splicing factor 1

Annotation symbol

CG5655

Feature type

protein_coding_gene

FlyBase ID

FBgn0011305

Gene Model Status

Current

Stock availability

2 publicly available

Also Known As

Rox21

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

31B1-31B1

Sequence location

2L:10,261,795..10,263,554 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Repressor splicing factor 1 is referred to in FlyBase by the symbol Dmel\Rsf1 (CG5655, FBgn0011305). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: mRNA binding. It is reported to be involved in the biological process: negative regulation of mRNA splicing, via spliceosome. 7 alleles are reported. The phenotypes of these alleles are annotated with: eye; retina; macrochaeta. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Nucleotide-binding, alpha-beta plait; RNA recognition motif domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1641116_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Rsf1. Gene sequence location is 2L:10261795..10263554.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

31B1-31B1

Limits computationally determined from genome sequence between P{lacW}me31B^k06607 and P{PZ}nmd⁰⁸⁷⁷⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

31C1-31D6

(determined by in situ hybridisation)

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Rsf1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Rsf1-RA

FBtr0080079

NM_057653

914

200

Rsf1-RB

FBtr0333411

NM_001273389

1760

200

Additional Transcript Data & Comments

Reported size (kB)

1.173 (longest cDNA)

(Brand et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Rsf1-PA

FBpp0079668

21.4

200

11.09

NP_477001

AAF52890

Rsf1-PB

FBpp0305603

21.4

200

11.09

NP_001260318

AGB92853

Additional Polypeptide Data & Comments

Reported size (kDa)

197 (aa); 21 (kD predicted)

(Brand et al., 1995)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RNA recognition motif domain (IPR000504)

Nucleotide-binding, alpha-beta plait (IPR012677)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q24491

UniProtKB/TrEMBL

Q8SYR3

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism | intense & ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | intense & ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | intense & ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

organism | intense & ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

nucleus

inferred from direct assay

(Allemand et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult female reproductive system, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1368.775
Larval Midgut		147.1
Larval Hindgut		316.8
Larval Malpighian Tubules		201
Larval Fat Body		362.1
Larval Salivary Gland		159.5
Larval Trachea		558.825
Larval Carcass		319.175
Adult Head		365.4
Adult Eye		754.775
Adult Brain		940.2
Adult Thoracic-Abdominal Ganglion		985.4
Adult Crop		310.5
Adult Midgut		175
Adult Hindgut		263.2
Adult Malpighian Tubules		153.1
Adult Fat Body		178.1
Adult Salivary Gland		290.7
Adult Heart		257.25
Adult VirginFemale Spermatheca		317.7
Adult InseminatedFemale Spermatheca		352.2
Adult Ovary		320.1
Adult Testis		150.3
Adult Male Accessory Gland		419.9
Adult Carcass		190
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1368.775)
Larval Midgut		147.1
Larval Hindgut		316.8
Larval Malpighian Tubules		201
Larval Fat Body		362.1
Larval Salivary Gland		159.5
Larval Trachea		(558.825)
Larval Carcass		319.175
Adult Head		365.4
Adult Eye		(754.775)
Adult Brain		(940.2)
Adult Thoracic-Abdominal Ganglion		(985.4)
Adult Crop		310.5
Adult Midgut		175
Adult Hindgut		263.2
Adult Malpighian Tubules		153.1
Adult Fat Body		178.1
Adult Salivary Gland		290.7
Adult Heart		257.25
Adult VirginFemale Spermatheca		317.7
Adult InseminatedFemale Spermatheca		352.2
Adult Ovary		320.1
Adult Testis		150.3
Adult Male Accessory Gland		419.9
Adult Carcass		190
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(1368.775)
Larval Midgut		147.1
Larval Hindgut		316.8
Larval Malpighian Tubules		201
Larval Fat Body		362.1
Larval Salivary Gland		159.5
Larval Trachea		558.825
Larval Carcass		319.175
Adult Head		365.4
Adult Eye		754.775
Adult Brain		940.2
Adult Thoracic-Abdominal Ganglion		985.4
Adult Crop		310.5
Adult Midgut		175
Adult Hindgut		263.2
Adult Malpighian Tubules		153.1
Adult Fat Body		178.1
Adult Salivary Gland		290.7
Adult Heart		257.25
Adult VirginFemale Spermatheca		317.7
Adult InseminatedFemale Spermatheca		352.2
Adult Ovary		320.1
Adult Testis		150.3
Adult Male Accessory Gland		419.9
Adult Carcass		190
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1368.775
Larval Midgut		147.1
Larval Hindgut		316.8
Larval Malpighian Tubules		201
Larval Fat Body		362.1
Larval Salivary Gland		159.5
Larval Trachea		558.825
Larval Carcass		319.175
Adult Head		365.4
Adult Eye		754.775
Adult Brain		940.2
Adult Thoracic-Abdominal Ganglion		985.4
Adult Crop		310.5
Adult Midgut		175
Adult Hindgut		263.2
Adult Malpighian Tubules		153.1
Adult Fat Body		178.1
Adult Salivary Gland		290.7
Adult Heart		257.25
Adult VirginFemale Spermatheca		317.7
Adult InseminatedFemale Spermatheca		352.2
Adult Ovary		320.1
Adult Testis		150.3
Adult Male Accessory Gland		419.9
Adult Carcass		190
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1368.775
Larval Midgut		147.1
Larval Hindgut		316.8
Larval Malpighian Tubules		201
Larval Fat Body		362.1
Larval Salivary Gland		159.5
Larval Trachea		558.825
Larval Carcass		319.175
Adult Head		365.4
Adult Eye		754.775
Adult Brain		940.2
Adult Thoracic-Abdominal Ganglion		985.4
Adult Crop		310.5
Adult Midgut		175
Adult Hindgut		263.2
Adult Malpighian Tubules		153.1
Adult Fat Body		178.1
Adult Salivary Gland		290.7
Adult Heart		257.25
Adult VirginFemale Spermatheca		317.7
Adult InseminatedFemale Spermatheca		352.2
Adult Ovary		320.1
Adult Testis		150.3
Adult Male Accessory Gland		419.9
Adult Carcass		190
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1368.775)
Larval Midgut		147.1
Larval Hindgut		316.8
Larval Malpighian Tubules		201
Larval Fat Body		362.1
Larval Salivary Gland		159.5
Larval Trachea		558.825
Larval Carcass		319.175
Adult Head		365.4
Adult Eye		754.775
Adult Brain		(940.2)
Adult Thoracic-Abdominal Ganglion		(985.4)
Adult Crop		310.5
Adult Midgut		175
Adult Hindgut		263.2
Adult Malpighian Tubules		153.1
Adult Fat Body		178.1
Adult Salivary Gland		290.7
Adult Heart		257.25
Adult VirginFemale Spermatheca		317.7
Adult InseminatedFemale Spermatheca		352.2
Adult Ovary		320.1
Adult Testis		150.3
Adult Male Accessory Gland		419.9
Adult Carcass		190
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		1368.775
Larval Midgut		147.1
Larval Hindgut		316.8
Larval Malpighian Tubules		201
Larval Fat Body		362.1
Larval Salivary Gland		159.5
Larval Trachea		558.825
Larval Carcass		319.175
Adult Head		365.4
Adult Eye		754.775
Adult Brain		940.2
Adult Thoracic-Abdominal Ganglion		985.4
Adult Crop		310.5
Adult Midgut		175
Adult Hindgut		263.2
Adult Malpighian Tubules		153.1
Adult Fat Body		178.1
Adult Salivary Gland		290.7
Adult Heart		257.25
Adult VirginFemale Spermatheca		317.7
Adult InseminatedFemale Spermatheca		352.2
Adult Ovary		320.1
Adult Testis		150.3
Adult Male Accessory Gland		419.9
Adult Carcass		190
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1368.775
Larval Midgut		147.1
Larval Hindgut		316.8
Larval Malpighian Tubules		201
Larval Fat Body		362.1
Larval Salivary Gland		159.5
Larval Trachea		558.825
Larval Carcass		319.175
Adult Head		365.4
Adult Eye		754.775
Adult Brain		940.2
Adult Thoracic-Abdominal Ganglion		985.4
Adult Crop		310.5
Adult Midgut		175
Adult Hindgut		263.2
Adult Malpighian Tubules		153.1
Adult Fat Body		178.1
Adult Salivary Gland		290.7
Adult Heart		257.25
Adult VirginFemale Spermatheca		317.7
Adult InseminatedFemale Spermatheca		352.2
Adult Ovary		320.1
Adult Testis		150.3
Adult Male Accessory Gland		419.9
Adult Carcass		190
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	365.4
NA	Eye	754.775
NA	Brain	940.2
1368.775	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	985.4
NA	Crop	310.5
147.1	Midgut	175
316.8	Hindgut	263.2
201	Malpighian Tubules	153.1
362.1	Fat Body	178.1
159.5	Salivary Gland	290.7
NA	Heart	257.25
558.825	Trachea	NA
NA	VirginFemale Spermatheca	317.7
NA	InseminatedFemale Spermatheca	352.2
NA	Ovary	320.1
NA	Testis	150.3
NA	Male Accessory Gland	419.9
319.175	Carcass	190

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		47
central nervous system, larvae L3		95
central nervous system, pupae P8		93
head, virgin 1-day female		16
head, virgin 4-day female		17
head, virgin 20-day female		11
head, mated 1-day female		17
head, mated 4-day female		16
head, mated 20-day female		12
head, mated 1-day male		23
head, mated 4-day male		29
head, mated 20-day male		23
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		8
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		33
carcass, 1-day adult		5
carcass, 4-day adult		6
carcass, 20-day adult		6
ovary, virgin 4-day female		15
ovary, mated 4-day female		12
testis, mated 4-day male		8
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(47)
central nervous system, larvae L3		(95)
central nervous system, pupae P8		(93)
head, virgin 1-day female		16
head, virgin 4-day female		17
head, virgin 20-day female		11
head, mated 1-day female		17
head, mated 4-day female		16
head, mated 20-day female		12
head, mated 1-day male		23
head, mated 4-day male		(29)
head, mated 20-day male		23
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		8
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		11
fat body, pupae P8		(42)
carcass, larvae L3 wandering		(33)
carcass, 1-day adult		5
carcass, 4-day adult		6
carcass, 20-day adult		6
ovary, virgin 4-day female		15
ovary, mated 4-day female		12
testis, mated 4-day male		8
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		47
central nervous system, larvae L3		95
central nervous system, pupae P8		93
head, virgin 1-day female		16
head, virgin 4-day female		17
head, virgin 20-day female		11
head, mated 1-day female		17
head, mated 4-day female		16
head, mated 20-day female		12
head, mated 1-day male		23
head, mated 4-day male		29
head, mated 20-day male		23
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		8
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		33
carcass, 1-day adult		5
carcass, 4-day adult		6
carcass, 20-day adult		6
ovary, virgin 4-day female		15
ovary, mated 4-day female		12
testis, mated 4-day male		8
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		47
central nervous system, larvae L3		95
central nervous system, pupae P8		93
head, virgin 1-day female		16
head, virgin 4-day female		17
head, virgin 20-day female		11
head, mated 1-day female		17
head, mated 4-day female		16
head, mated 20-day female		12
head, mated 1-day male		23
head, mated 4-day male		29
head, mated 20-day male		23
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		8
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		33
carcass, 1-day adult		5
carcass, 4-day adult		6
carcass, 20-day adult		6
ovary, virgin 4-day female		15
ovary, mated 4-day female		12
testis, mated 4-day male		8
accessory gland, mated 4-day male		11
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		47
central nervous system, larvae L3		95
central nervous system, pupae P8		93
head, virgin 1-day female		16
head, virgin 4-day female		17
head, virgin 20-day female		11
head, mated 1-day female		17
head, mated 4-day female		16
head, mated 20-day female		12
head, mated 1-day male		23
head, mated 4-day male		29
head, mated 20-day male		23
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		8
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		33
carcass, 1-day adult		5
carcass, 4-day adult		6
carcass, 20-day adult		6
ovary, virgin 4-day female		15
ovary, mated 4-day female		12
testis, mated 4-day male		8
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(47)
central nervous system, larvae L3		(95)
central nervous system, pupae P8		(93)
head, virgin 1-day female		16
head, virgin 4-day female		17
head, virgin 20-day female		11
head, mated 1-day female		17
head, mated 4-day female		16
head, mated 20-day female		12
head, mated 1-day male		23
head, mated 4-day male		29
head, mated 20-day male		23
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		8
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		11
fat body, pupae P8		(42)
carcass, larvae L3 wandering		(33)
carcass, 1-day adult		5
carcass, 4-day adult		6
carcass, 20-day adult		6
ovary, virgin 4-day female		15
ovary, mated 4-day female		12
testis, mated 4-day male		8
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		47
central nervous system, larvae L3		95
central nervous system, pupae P8		93
head, virgin 1-day female		16
head, virgin 4-day female		17
head, virgin 20-day female		11
head, mated 1-day female		17
head, mated 4-day female		16
head, mated 20-day female		12
head, mated 1-day male		23
head, mated 4-day male		29
head, mated 20-day male		23
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		8
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		33
carcass, 1-day adult		5
carcass, 4-day adult		6
carcass, 20-day adult		6
ovary, virgin 4-day female		15
ovary, mated 4-day female		12
testis, mated 4-day male		8
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		47
central nervous system, larvae L3		95
central nervous system, pupae P8		93
head, virgin 1-day female		16
head, virgin 4-day female		17
head, virgin 20-day female		11
head, mated 1-day female		17
head, mated 4-day female		16
head, mated 20-day female		12
head, mated 1-day male		23
head, mated 4-day male		29
head, mated 20-day male		23
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		6
digestive system, larvae L3 wandering		8
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		4
fat body, larvae L3 wandering		4
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		33
carcass, 1-day adult		5
carcass, 4-day adult		6
carcass, 20-day adult		6
ovary, virgin 4-day female		15
ovary, mated 4-day female		12
testis, mated 4-day male		8
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		44
embryo 04-06hr		67
embryo 06-08hr		70
embryo 08-10hr		60
embryo 10-12hr		60
embryo 12-14hr		42
embryo 14-16hr		39
embryo 16-18hr		27
embryo 18-20hr		18
embryo 20-22hr		16
embryo 22-24hr		15
larva L1		14
larva L2		13
larva L3 12hr old		7
larva L3 puffstage 1-2		16
larva L3 puffstage 3-6		28
larva L3 puffstage 7-9		39
white prepupae new		47
white prepupae 12hr		46
white prepupae 24hr		63
pupae 2d postWPP		50
pupae 3d postWPP		20
pupae 4d postWPP		10
adult male 01day		12
adult male 05day		15
adult male 30day		13
adult female 01day		11
adult female 05day		13
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		(44)
embryo 04-06hr		(67)
embryo 06-08hr		(70)
embryo 08-10hr		(60)
embryo 10-12hr		(60)
embryo 12-14hr		(42)
embryo 14-16hr		(39)
embryo 16-18hr		(27)
embryo 18-20hr		18
embryo 20-22hr		16
embryo 22-24hr		15
larva L1		14
larva L2		13
larva L3 12hr old		7
larva L3 puffstage 1-2		16
larva L3 puffstage 3-6		(28)
larva L3 puffstage 7-9		(39)
white prepupae new		(47)
white prepupae 12hr		(46)
white prepupae 24hr		(63)
pupae 2d postWPP		(50)
pupae 3d postWPP		20
pupae 4d postWPP		10
adult male 01day		12
adult male 05day		15
adult male 30day		13
adult female 01day		11
adult female 05day		13
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		44
embryo 04-06hr		67
embryo 06-08hr		70
embryo 08-10hr		60
embryo 10-12hr		60
embryo 12-14hr		42
embryo 14-16hr		39
embryo 16-18hr		27
embryo 18-20hr		18
embryo 20-22hr		16
embryo 22-24hr		15
larva L1		14
larva L2		13
larva L3 12hr old		7
larva L3 puffstage 1-2		16
larva L3 puffstage 3-6		28
larva L3 puffstage 7-9		39
white prepupae new		47
white prepupae 12hr		46
white prepupae 24hr		63
pupae 2d postWPP		50
pupae 3d postWPP		20
pupae 4d postWPP		10
adult male 01day		12
adult male 05day		15
adult male 30day		13
adult female 01day		11
adult female 05day		13
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		44
embryo 04-06hr		67
embryo 06-08hr		70
embryo 08-10hr		60
embryo 10-12hr		60
embryo 12-14hr		42
embryo 14-16hr		39
embryo 16-18hr		27
embryo 18-20hr		18
embryo 20-22hr		16
embryo 22-24hr		15
larva L1		14
larva L2		13
larva L3 12hr old		7
larva L3 puffstage 1-2		16
larva L3 puffstage 3-6		28
larva L3 puffstage 7-9		39
white prepupae new		47
white prepupae 12hr		46
white prepupae 24hr		63
pupae 2d postWPP		50
pupae 3d postWPP		20
pupae 4d postWPP		10
adult male 01day		12
adult male 05day		15
adult male 30day		13
adult female 01day		11
adult female 05day		13
adult female 30day		15
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		44
embryo 04-06hr		67
embryo 06-08hr		70
embryo 08-10hr		60
embryo 10-12hr		60
embryo 12-14hr		42
embryo 14-16hr		39
embryo 16-18hr		27
embryo 18-20hr		18
embryo 20-22hr		16
embryo 22-24hr		15
larva L1		14
larva L2		13
larva L3 12hr old		7
larva L3 puffstage 1-2		16
larva L3 puffstage 3-6		28
larva L3 puffstage 7-9		39
white prepupae new		47
white prepupae 12hr		46
white prepupae 24hr		63
pupae 2d postWPP		50
pupae 3d postWPP		20
pupae 4d postWPP		10
adult male 01day		12
adult male 05day		15
adult male 30day		13
adult female 01day		11
adult female 05day		13
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		(44)
embryo 04-06hr		(67)
embryo 06-08hr		(70)
embryo 08-10hr		(60)
embryo 10-12hr		(60)
embryo 12-14hr		(42)
embryo 14-16hr		(39)
embryo 16-18hr		27
embryo 18-20hr		18
embryo 20-22hr		16
embryo 22-24hr		15
larva L1		14
larva L2		13
larva L3 12hr old		7
larva L3 puffstage 1-2		16
larva L3 puffstage 3-6		28
larva L3 puffstage 7-9		(39)
white prepupae new		(47)
white prepupae 12hr		(46)
white prepupae 24hr		(63)
pupae 2d postWPP		(50)
pupae 3d postWPP		20
pupae 4d postWPP		10
adult male 01day		12
adult male 05day		15
adult male 30day		13
adult female 01day		11
adult female 05day		13
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		44
embryo 04-06hr		67
embryo 06-08hr		70
embryo 08-10hr		60
embryo 10-12hr		60
embryo 12-14hr		42
embryo 14-16hr		39
embryo 16-18hr		27
embryo 18-20hr		18
embryo 20-22hr		16
embryo 22-24hr		15
larva L1		14
larva L2		13
larva L3 12hr old		7
larva L3 puffstage 1-2		16
larva L3 puffstage 3-6		28
larva L3 puffstage 7-9		39
white prepupae new		47
white prepupae 12hr		46
white prepupae 24hr		63
pupae 2d postWPP		50
pupae 3d postWPP		20
pupae 4d postWPP		10
adult male 01day		12
adult male 05day		15
adult male 30day		13
adult female 01day		11
adult female 05day		13
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		23
embryo 02-04hr		44
embryo 04-06hr		67
embryo 06-08hr		70
embryo 08-10hr		60
embryo 10-12hr		60
embryo 12-14hr		42
embryo 14-16hr		39
embryo 16-18hr		27
embryo 18-20hr		18
embryo 20-22hr		16
embryo 22-24hr		15
larva L1		14
larva L2		13
larva L3 12hr old		7
larva L3 puffstage 1-2		16
larva L3 puffstage 3-6		28
larva L3 puffstage 7-9		39
white prepupae new		47
white prepupae 12hr		46
white prepupae 24hr		63
pupae 2d postWPP		50
pupae 3d postWPP		20
pupae 4d postWPP		10
adult male 01day		12
adult male 05day		15
adult male 30day		13
adult female 01day		11
adult female 05day		13
adult female 30day		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		25
Schneider line 2 Sg4		23
embryonic 1182-4H		14
embryonic GM2		22
embryonic Kc167		20
embryonic S1		33
embryonic S3		28
leg disc CME L1		17
wing disc CME-W2		27
wing disc ML-DmD8		39
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		38
wing disc ML-DmD21		62
wing disc ML-DmD32		45
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		29
antennal disc ML-DmD20-c5		39
mixed discs ML-DmD4-c1		54
CNS ML-DmBG1-c1		40
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		27
ovary fGS/OSS		23
ovary OSC		46
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		25
Schneider line 2 Sg4		23
embryonic 1182-4H		14
embryonic GM2		22
embryonic Kc167		20
embryonic S1		(33)
embryonic S3		(28)
leg disc CME L1		17
wing disc CME-W2		(27)
wing disc ML-DmD8		(39)
wing disc ML-DmD9		(37)
wing disc ML-DmD16-c3		(38)
wing disc ML-DmD21		(62)
wing disc ML-DmD32		(45)
haltere disc ML-DmD17-c3		(29)
eye-antennal disc ML-DmD11		(29)
antennal disc ML-DmD20-c5		(39)
mixed discs ML-DmD4-c1		(54)
CNS ML-DmBG1-c1		(40)
CNS ML-DmBG2-c2		(38)
tumorous blood cells mbn2		(27)
ovary fGS/OSS		23
ovary OSC		(46)
ovary OSS		(33)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		25
Schneider line 2 Sg4		23
embryonic 1182-4H		14
embryonic GM2		22
embryonic Kc167		20
embryonic S1		33
embryonic S3		28
leg disc CME L1		17
wing disc CME-W2		27
wing disc ML-DmD8		39
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		38
wing disc ML-DmD21		62
wing disc ML-DmD32		45
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		29
antennal disc ML-DmD20-c5		39
mixed discs ML-DmD4-c1		54
CNS ML-DmBG1-c1		40
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		27
ovary fGS/OSS		23
ovary OSC		46
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		25
Schneider line 2 Sg4		23
embryonic 1182-4H		14
embryonic GM2		22
embryonic Kc167		20
embryonic S1		33
embryonic S3		28
leg disc CME L1		17
wing disc CME-W2		27
wing disc ML-DmD8		39
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		38
wing disc ML-DmD21		62
wing disc ML-DmD32		45
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		29
antennal disc ML-DmD20-c5		39
mixed discs ML-DmD4-c1		54
CNS ML-DmBG1-c1		40
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		27
ovary fGS/OSS		23
ovary OSC		46
ovary OSS		33
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		25
Schneider line 2 Sg4		23
embryonic 1182-4H		14
embryonic GM2		22
embryonic Kc167		20
embryonic S1		33
embryonic S3		28
leg disc CME L1		17
wing disc CME-W2		27
wing disc ML-DmD8		39
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		38
wing disc ML-DmD21		62
wing disc ML-DmD32		45
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		29
antennal disc ML-DmD20-c5		39
mixed discs ML-DmD4-c1		54
CNS ML-DmBG1-c1		40
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		27
ovary fGS/OSS		23
ovary OSC		46
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		25
Schneider line 2 Sg4		23
embryonic 1182-4H		14
embryonic GM2		22
embryonic Kc167		20
embryonic S1		(33)
embryonic S3		28
leg disc CME L1		17
wing disc CME-W2		27
wing disc ML-DmD8		(39)
wing disc ML-DmD9		(37)
wing disc ML-DmD16-c3		(38)
wing disc ML-DmD21		(62)
wing disc ML-DmD32		(45)
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		29
antennal disc ML-DmD20-c5		(39)
mixed discs ML-DmD4-c1		(54)
CNS ML-DmBG1-c1		(40)
CNS ML-DmBG2-c2		(38)
tumorous blood cells mbn2		27
ovary fGS/OSS		23
ovary OSC		(46)
ovary OSS		(33)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		25
Schneider line 2 Sg4		23
embryonic 1182-4H		14
embryonic GM2		22
embryonic Kc167		20
embryonic S1		33
embryonic S3		28
leg disc CME L1		17
wing disc CME-W2		27
wing disc ML-DmD8		39
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		38
wing disc ML-DmD21		62
wing disc ML-DmD32		45
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		29
antennal disc ML-DmD20-c5		39
mixed discs ML-DmD4-c1		54
CNS ML-DmBG1-c1		40
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		27
ovary fGS/OSS		23
ovary OSC		46
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		25
Schneider line 2 Sg4		23
embryonic 1182-4H		14
embryonic GM2		22
embryonic Kc167		20
embryonic S1		33
embryonic S3		28
leg disc CME L1		17
wing disc CME-W2		27
wing disc ML-DmD8		39
wing disc ML-DmD9		37
wing disc ML-DmD16-c3		38
wing disc ML-DmD21		62
wing disc ML-DmD32		45
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		29
antennal disc ML-DmD20-c5		39
mixed discs ML-DmD4-c1		54
CNS ML-DmBG1-c1		40
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		27
ovary fGS/OSS		23
ovary OSC		46
ovary OSS		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		7
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		17
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		12
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		26
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		15
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		12
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		7
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		17
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		12
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		26
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		15
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		12
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		7
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		17
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		12
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		26
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		15
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		12
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		7
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		17
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		12
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		26
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		15
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		12
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		7
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		17
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		12
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		26
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		15
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		12
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		7
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		17
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		12
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		26
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		15
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		12
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		7
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		17
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		12
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		26
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		15
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		12
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		8
cold shock, 4-day adult		7
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		17
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		12
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		26
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		8
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		15
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		12
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

RE39606

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0011305

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG5655-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Rsf1

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | embryonic stage, with Scer\GAL4^da.G32

Rsf1^Scer\UAS.cLa

lethal | partially, with Scer\GAL4^GMR.PF

Rsf1^Scer\UAS.cLa

lethal | pupal stage, with Scer\GAL4^sca-537.4

Rsf1^Scer\UAS.cLa

viable, with Scer\GAL4^pnr-MD237

Rsf1^GD11723

Other Phenotypes

Allele

visible, with Scer\GAL4^GMR.PF

Rsf1^Scer\UAS.cLa

visible, with Scer\GAL4^sca-537.4

Rsf1^Scer\UAS.cLa

Phenotype manifest in

Allele

eye, with Scer\GAL4^GMR.PF

Rsf1^Scer\UAS.cLa

macrochaeta, with Scer\GAL4^sca-537.4

Rsf1^Scer\UAS.cLa

retina, with Scer\GAL4^GMR.PF

Rsf1^Scer\UAS.cLa

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Rsf1	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Rsf1	Mutagen	Stocks	Known lesion
Rsf1^GD11723	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Rsf1^HMS02368	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Rsf1^NIG.5655R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Rsf1^Scer\UAS.cLa	in vitro construct - regulatory fusion	0	Yes
Rsf1^{T:Avic\GFP-EGFP}	in vitro construct - coding region fusion	0	Yes
Rsf1^{ΔGRS.T:Avic\GFP-EGFP}	in vitro construct - coding region fusion	0	Yes
Rsf1^{ΔRRM.T:Avic\GFP-EGFP}	in vitro construct - coding region fusion	0	Yes