FlyBase Gene Report: Dmel\ND-ACP

General Information

Symbol

Dmel\ND-ACP

Species

D. melanogaster

Name

NADH dehydrogenase (ubiquinone) acyl carrier protein

Annotation Symbol

CG9160

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011361

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Carrier of the growing fatty acid chain in fatty acid biosynthesis. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain (By similarity). (UniProt, Q94519)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

mtacp1, mtACP, NADH-ubiquinone oxidoreductase acyl-carrier subunit, mitochondrial acyl carrier protein 1, dNDUFAB1

Key Links

Genomic Location

Cytogenetic map

61F6-61F6

Sequence location

3L:1,329,949..1,331,631 [+]

Recombination map

3-0

RefSeq locus

NT_037436 REGION:1329949..1331631

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables acyl binding

inferred from biological aspect of ancestor with PANTHER:PTN000466551

(GO Reference Genome Project, 2011-)

enables acyl carrier activity

inferred from biological aspect of ancestor with PANTHER:PTN000466551

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in fatty acid biosynthetic process

inferred from electronic annotation with InterPro:IPR003231

(InterPro Project Members, 2004-)

involved_in mitochondrial electron transport, NADH to ubiquinone

inferred by curator from GO:0045271

(Agip et al., 2023)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of respiratory chain complex I

inferred from direct assay

(Agip et al., 2023)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

part_of mitochondrial [2Fe-2S] assembly complex

inferred from sequence or structural similarity with UniProtKB:O14561

(Gene Ontology Curators, 2002-)

is_active_in mitochondrial inner membrane

inferred by curator from GO:0045271

(Agip et al., 2023)

is_active_in mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000466616

(GO Reference Genome Project, 2011-)

located_in mitochondrion

inferred from sequence alignment with UniProtKB:P52505

(Ragone et al., 1999)

part_of respiratory chain complex I

inferred from sequence or structural similarity with UniProtKB:O14561

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN001074652

(GO Reference Genome Project, 2011-)

non-traceable author statement

(Porcelli et al., 2007)

Gene Group (FlyBase)

MITOCHONDRIAL COMPLEX I - ACCESSORY SUBUNITS

MITOCHONDRIAL COMPLEX I - TESTIS-SPECIFIC VARIANT

MITOCHONDRIAL COMPLEX I

MITOCHONDRIAL IRON-SULFUR ASSEMBLY COMPLEX

Protein Family (UniProt)

Belongs to the acyl carrier protein (ACP) family. (Q94519)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

MITOCHONDRIAL COMPLEX I - ACCESSORY SUBUNITS -: Mitochondrial Complex I is the first and largest holoenzyme of the mitochondrial electron transport chain. It oxidizes NADH from the tricarboxylic acid cycle and β oxidation of fatty acids, reduces ubiquinone and transports protons across the inner membrane, contributing to the proton-motive force. It is also involved in the cellular production of reactive oxygen species. 'Accessory' or 'supernumerary' subunits are all encoded by nuclear DNA and are not directly involved in catalysis. (Adapted from PMID:23527692 and FBrf0244962.)
MITOCHONDRIAL COMPLEX I - TESTIS-SPECIFIC VARIANT -: Mitochondrial Complex I is the first and largest holoenzyme of the mitochondrial electron transport chain. It oxidizes NADH from the tricarboxylic acid cycle and β oxidation of fatty acids, reduces ubiquinone and transports protons across the inner membrane, contributing to the proton-motive force. It is also involved in the cellular production of reactive oxygen species. Fourteen distinct subunits form the catalytic centers of the enzyme as they are directly involved in transferring electrons from NADH to ubiquinone, or for generation of the membrane potential. (Adapted from PMID:23527692 and FBrf0244962.)
MITOCHONDRIAL COMPLEX I -: Mitochondrial Complex I is the first and largest holoenzyme of the mitochondrial electron transport chain. It oxidizes NADH from the tricarboxylic acid cycle and β oxidation of fatty acids, reduces ubiquinone and transports protons across the inner membrane, contributing to the proton-motive force. It is also involved in the cellular production of reactive oxygen species. Fourteen distinct subunits form the catalytic centers of the enzyme as they are directly involved in transferring electrons from NADH to ubiquinone, or for generation of the membrane potential. (Adapted from PMID:23527692 and FBrf0244962.)
MITOCHONDRIAL IRON-SULFUR ASSEMBLY COMPLEX -: The Mitochondrial iron-sulfur assembly complex is the core complex involved in the synthesis of organometallic Fe-S clusters in the mitochondrial matrix. Iron-sulfur clusters are incorporated into various proteins and are required for a variety of processes such as electron transfer, catalysis and structural roles. (Adapted from FBrf0238243.)

Protein Function (UniProtKB)

(UniProt, Q94519)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ND-ACP for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q94519)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ND-ACP-RA

FBtr0072676

NM_057655

740

152

ND-ACP-RB

FBtr0072677

NM_057654

625

143

ND-ACP-RC

FBtr0331864

NM_001274321

739

181

Additional Transcript Data and Comments

Reported size (kB)

0.8 (unknown)

(Ragone et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ND-ACP-PA

FBpp0072569

17.3

152

6.79

NP_477003

AAF47480

ND-ACP-PB

FBpp0072570

16.0

143

5.70

FBpp0304248

20.6

181

9.04

M9PDU4

NP_001261250

AGB93945

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

152, 143 (aa)

(Ragone et al., 1999)

Comments

External Data

Subunit Structure (UniProtKB)

Complex I is composed of about 45 different subunits.

(UniProt, Q94519)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ND-ACP using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mtacp1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.17

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic midgut chamber

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Ragone et al., 1999)

embryonic stage 14 -- 17

embryonic/larval tracheal dorsal trunk

(Ragone et al., 1999)

larval tracheal system

(Ragone et al., 1999)

embryonic stage 15 -- 17

embryonic/larval tracheal lateral trunk

(Ragone et al., 1999)

adult stage oogenesis stage S10 -- S11

nurse cell

(Ragone et al., 1999)

Additional Descriptive Data

Maternally derived ND-ACP transcripts are detected in 0-3hr embryos on northern blots. Zygotic transcripts appear between 6 and 9 hours of embryogenesis and accumulate throughout late embryogenesis. Transcripts decrease in larval stages, increase again in pupae and persist into adulthood. Transcripts are detected by in situ hybridization in the tracheal system. At embryonic stage 14, transcripts are found in the dorsal tracheal trunks. By stage 15, staining is also strong in the lateral tracheal trunks. By stage 16, staining is evident further into the tracheal system. During oogenesis, transcripts are detected at stage S10/11 in nurse cells. Probes specific to each of the two ND-ACP splice variants detect the same pattern of transcription.

(Ragone et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of respiratory chain complex I

inferred from direct assay

(Agip et al., 2023)

Expression Deduced from Reporters

Reporter: P{lacW}ND-ACP^j4A6

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval midgut | restricted

(Meister and Braun, 1995.10)

gonad

(Meister and Braun, 1995.10)

embryonic/larval foregut

(Meister and Braun, 1995.10)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ND-ACP in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ND-ACP

View exonic expression by tissue for Dmel\ND-ACP

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011361

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38154/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011361

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011361

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011361

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9160

Flygut - An atlas of the Drosophila adult midgut

FBgn0011361

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ND-ACP

Stages(s) 1-3
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ND-ACP

Transgenic constructs containing regulatory region of ND-ACP

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(3L)Ar14-8

(Spradling et al., 1999)

Df(3L)bab-PG

(Spradling et al., 1999)

Df(3L)BSC250

(Gresens et al., 2007.3.22)

Gene partially disrupted in

Df(3L)920

(Hegan et al., 2007)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

ND-ACP^GD15185

lethal (with Df(3L)3C7)

ND-ACP¹³⁰³

lethal (with Df(3L)bab-PG)

ND-ACP¹³⁰³

lethal | recessive

ND-ACP^j4A6

lethal - all die before end of first instar larval stage | recessive

ND-ACP⁹²⁰

lethal - all die before end of larval stage | recessive

ND-ACP¹³⁰³

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

ND-ACP^GD15398

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ND-ACP^GD15398

partially lethal - majority die, with Scer\GAL4^pnr-MD237

ND-ACP^GD15185

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ND-ACP^GD15185

viable, with Scer\GAL4^pnr-MD237

ND-ACP^GD15398

viable, with Scer\GAL4^tin.CΔ4

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ND-ACP^GD15185

abnormal size | adult stage, with Scer\GAL4^eya.enh1+E+2

ND-ACP^HMS01634

some die during first instar larval stage | recessive

ND-ACP⁹²⁰

some die during larval stage | recessive

ND-ACP¹³⁰³

some die during pupal stage, with Scer\GAL4^pnr-MD237

ND-ACP^GD15185

visible, with Scer\GAL4^pnr-MD237

visible | adult stage, with Scer\GAL4^eya.enh1+E+2

Phenotype manifest in

Allele

eye, with Scer\GAL4^eya.enh1+E+2

germarium

ND-ACP^j4A6

mesonotum, with Scer\GAL4^pnr-MD237

onion stage spermatid

ND-ACP^j4A6

spermatid

ND-ACP^j4A6

tracheal section

ND-ACP^j4A6

trichogen cell, with Scer\GAL4^pnr-MD237

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\NDUFAB1

11 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Ndufab1

12 of 14

Yes

Rnor\Fasn

1 of 14

Mus musculus (laboratory mouse) (2)

Mmus\Ndufab1

11 of 14

Yes

Mmus\Fasn

1 of 14

Xenopus tropicalis (Western clawed frog) (1)

Xtro\ndufab1

10 of 13

Yes

Danio rerio (Zebrafish) (2)

Drer\ndufab1a

12 of 14

Yes

Drer\ndufab1b

12 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\Y56A3A.19

13 of 14

Yes

Cele\F37C12.3

6 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP010464

10 of 12

Yes

Agam\AgaP_AGAP008468

1 of 12

Arabidopsis thaliana (thale-cress) (9)

Atha\MTACP-1

11 of 13

Yes

Atha\mtACP2

10 of 13

Yes

Atha\mtACP3

6 of 13

Yes

Atha\ACP4

4 of 13

Yes

Atha\ACP1

3 of 13

Yes

Atha\ACP2

3 of 13

Yes

Atha\ACP3

3 of 13

Yes

Atha\ACP5

3 of 13

Yes

Atha\AT5G36280

3 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ACP1

8 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC4H3.09

9 of 12

Yes

Escherichia coli (enterobacterium) (1)

Ecol\acpP

8 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ND-ACP. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG3224

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

neurodegenerative disease of CoA-mtACP-PDH pathway, NDUFAB1 model

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

ND-ACP^HM05206

model of neurodegeneration with brain iron accumulation

CEC

(Lambrechts et al., 2019)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NDUFAB1; NADH:ubiquinone oxidoreductase subunit AB1

11 of 14

603836

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

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Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ND-ACP - Lipt2

experimental knowledge based

(Guruharsha et al., 2011)

ND-ACP - ND-13A

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-13B

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-15

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-19

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-20

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-23

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-24

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-30

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-39

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-42

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-51

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-75

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-AGGG

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-ASHI

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B12

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B14

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B14.5A

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B14.5B

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B14.7

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B15

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B16.6

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B17

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B17.2

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B18

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-B22

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-MNLL

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-MWFE

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-PDSW

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - ND-SGDH

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - NP15.6

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - NdufA3

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - bcn92

experimental knowledge based

(Guruharsha et al., 2011)

ND-ACP - mod(mdg4)

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Hu et al., 2017.6.13, Golovnin et al., 2016)

ND-ACP - mt:ND1

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - mt:ND2

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - mt:ND3

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - mt:ND4L

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - mt:ND5

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

ND-ACP - mt:ND6

cosedimentation, electron microscopy, molecular weight estimation by staining

(Padavannil et al., 2023)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Complex I is composed of about 45 different subunits.

(UniProt, Q94519 )

Linkouts

BioGRID - A database of protein and genetic interactions.

63697

DroID - A comprehensive database of gene and protein interactions.

FBgn0011361

MIST (protein-protein) - An integrated Molecular Interaction Database

38154

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-0

Cytogenetic map

61F6-61F6

Sequence location

3L:1,329,949..1,331,631 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

61F6-61F6

Limits computationally determined from genome sequence between P{PZ}l(3)05967⁰⁵⁹⁶⁷ and P{PZ}l(3)02640⁰²⁶⁴⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

61F-61F

(Caggese, 1997.7.29)

61F1-61F8

(Caggese, 1996.10.24)

61F6-61F7

(determined by in situ hybridisation)

(Spradling et al., 1999)

61F6-61F8

(determined by in situ hybridisation)

(Caggese et al., 1999, Ragone et al., 1999)

61F6-61F7

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-0

(FlyBase, 2016)

3-1.7

(Comeron et al., 2012)

(Caggese, 1996.10.24)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

29528

y¹ v¹; P{TRiP.HM05206}attP2

7291

ND-ACP¹³⁰³ vn¹ st¹/TM6B, Tb¹

12061

y¹ w^*; P{lacW}ND-ACP^j4A6/TM6B, Tb⁺

83503

y¹ sc^* v¹ sev²¹; P{TKO.GS04223}attP40

37492

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01634}attP2

Kyoto

123701

w¹¹¹⁸; P{RS3}ND-ACP^CB-5593-3

111378

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}ND-ACP^j4A6 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹

VDRC

v107907

P{KK107702}VIE-260B

v39232

w¹¹¹⁸; P{GD15398}v39232

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (42)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011361

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011361

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Murari et al., 2023)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

mtacp1 is essential for viability and is required for both male and female gametogenesis.

(Ragone et al., 1999)

Relationship to Other Genes

Source for database merge of

Source for merge of: mtacp1 l(3)j4A6

(Ragone et al., 1999)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: ND-ACP mtacp1

(Porcelli and Marygold, 2014.9.16)

Nomenclature comments

Renamed from 'mtacp1' to 'ND-ACP' to match the format of other genes encoding NADH dehydrogenase (ubiquinone) subunits.

(Porcelli and Marygold, 2014.9.16)

Etymology

Synonyms and Secondary IDs (27)

Reported As

Symbol Synonym

ACPM

(Caggese et al., 2006)

CG9160

(Curcio et al., 2024, Murari et al., 2023, Rhooms et al., 2020, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Lu et al., 2014, Banerjee et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Tiefenböck et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Porcelli et al., 2007, Caggese et al., 2006, Qin et al., 2005, Tripoli et al., 2005)

MTACP1A

(Golovnin et al., 2016)

MTACP1B

(Golovnin et al., 2016)

ND-ACP

(Curcio et al., 2024, Groen et al., 2022, Li et al., 2022, Parker et al., 2020, Toshniwal et al., 2019, Hu et al., 2017.6.13, Transgenic RNAi Project members, 2017-, Porcelli and Marygold, 2014.9.16)

ND-AcC

(Spradling et al., 1999)

NDUFAB1

(Agip et al., 2023, Towarnicki and Ballard, 2020)

dNDUFAB1

(Murari et al., 2023, Rhooms et al., 2020)

l(3)1303

(Wei et al., 2003)

l(3)j4A6

mtACP

(Banerjee et al., 2012, Xu et al., 2006, Tripoli et al., 2005, Caggese, 1997.7.29)

mtACP1

(Toshniwal et al., 2019, Golovnin et al., 2016, Tiefenböck et al., 2010, Tripoli et al., 2005)

mtACP1A

(Golovnin et al., 2016)

mtACP1B

(Golovnin et al., 2016)

mtacp

(Lambrechts et al., 2019)

Name Synonyms

Acyl carrier protein

(Caggese et al., 2006)

Mitochondrial acyl carrier protein 1

(Qin et al., 2005)

NADH dehydrogenase (ubiquinone) acyl carrier protein

(Porcelli and Marygold, 2014.9.16)

NADH ubiquinone oxidoreductase ACP

(Porcelli et al., 2007)

NADH ubiquinone oxidoreductase acyl carrier protein

(Tripoli et al., 2005)

NADH-ubiquinone oxidoreductase acyl carrier protein

(Porcelli et al., 2007, Tripoli et al., 2005)

NADH-ubiquinone oxidoreductase acyl-carrier subunit

(Caggese, 1996.10.24, Caggese, 1996.10.24)

NADH:ubiquinone oxidoreductase Acyl carrier

(Tripoli et al., 2005)

mitochondrial acyl carrier protein

(Banerjee et al., 2012)

mitochondrial acyl carrier protein 1

(Cammarato et al., 2011, Wasbrough et al., 2010, Ragone et al., 1999)

mitochondrial acyl carrier protein of electron transport complex I

(Tiefenböck et al., 2010)

Secondary FlyBase IDs

FBgn0017568

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 64 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38154

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q94519

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q94519

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PDU4

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q94519

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011361

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38154/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011361

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011361

FlyMine - An integrated database for Drosophila genomics

FBgn0011361

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9160

MARRVEL_MODEL - MARRVEL (model organism gene)

38154

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

63697

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011361

DroID - A comprehensive database of gene and protein interactions.

FBgn0011361

DRSC - Results frm RNAi screens

FBgn0011361

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mtacp1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011361

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011361

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9160

Flygut - An atlas of the Drosophila adult midgut

FBgn0011361

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0011361

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034139

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

38154

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (94)

The following external sites may use different assemblies or annotations than FlyBase.

1.17

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