FB2025_05 , released December 11, 2025
Gene: Dmel\Klp3A
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General Information
Symbol
Dmel\Klp3A
Species
D. melanogaster
Name
Kinesin-like protein at 3A
Annotation Symbol
CG8590
Feature Type
protein_coding_gene
FlyBase ID
FBgn0011606
Gene Model Status
Current
Stock Availability
12 publicly available
Enzyme Name (EC)
plus-end-directed kinesin ATPase (5.6.1.3)
Gene Summary
Kinesin-like protein at 3A (Klp3A) encodes a microtubule motor protein involved in chromosome segregation and mitotic spindle morphogenesis. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)
All Summaries
Also Known As

mei-352, DmKlp3A, EG:BACR25B3.9 , fs(1)M4

Key Links
Genomic Location
Cytogenetic map
3A6-3A6
Sequence location
Recombination map
1-1
RefSeq locus
NC_004354 REGION:2599158..2604052
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (22 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables microtubule binding
inferred from direct assay
(Kwon et al., 2004, Sisson et al., 2000)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821
(InterPro Project Members, 2004-)
enables microtubule binding
inferred from electronic annotation with InterPro:IPR001752
(InterPro Project Members, 2004-)
enables microtubule motor activity
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821, InterPro:IPR027640
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN002516211
(GO Reference Genome Project, 2011-)
enables plus-end-directed microtubule motor activity
non-traceable author statement
(Mazumdar and Mazumdar, 2002)
Biological Process (11 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
involved_in chromosome segregation
inferred from mutant phenotype
(Kwon et al., 2004)
involved_in female meiosis chromosome segregation
inferred from mutant phenotype
(Baker and Carpenter, 1972)
involved_in male meiotic nuclear division
inferred from mutant phenotype
(Williams et al., 1995)
involved_in meiosis I cytokinesis
inferred from mutant phenotype
(Williams et al., 1995)
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
(Ducat et al., 2008)
involved_in mitotic spindle organization
inferred from mutant phenotype
(Kwon et al., 2004)
involved_in reciprocal meiotic recombination
inferred from mutant phenotype
(Baker and Carpenter, 1972)
involved_in regulation of mitotic nuclear division
inferred from mutant phenotype
(Kwon et al., 2004)
involved_in single fertilization
inferred from mutant phenotype
(Williams et al., 1997)
involved_in spindle elongation
inferred from mutant phenotype
(Kwon et al., 2004)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in male meiotic nuclear division
traceable author statement
(Mazumdar and Mazumdar, 2002)
involved_in microtubule-based movement
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821, InterPro:IPR027640
(InterPro Project Members, 2004-)
involved_in mitotic spindle organization
inferred from biological aspect of ancestor with PANTHER:PTN002516211
(GO Reference Genome Project, 2011-)
involved_in spindle elongation
inferred from biological aspect of ancestor with PANTHER:PTN002516211
(GO Reference Genome Project, 2011-)
Cellular Component (7 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
located_in aster
inferred from direct assay
(Williams et al., 1997)
located_in cytoplasm
inferred from direct assay
(Williams et al., 1995)
part_of microtubule associated complex
inferred from direct assay
(Williams et al., 1997)
located_in midbody
inferred from direct assay
(Kwon et al., 2004)
located_in nucleus
inferred from direct assay
(Kwon et al., 2004, Williams et al., 1995)
located_in spindle midzone
inferred from direct assay
(Kwon et al., 2004)
located_in spindle
inferred from direct assay
(Williams et al., 1997, Williams et al., 1995)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of microtubule associated complex
inferred from biological aspect of ancestor with PANTHER:PTN002516211
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
plus-end-directed microtubule motor activity
ATP + H2O + a kinesin associated with a microtubule at position (n) = ADP + phosphate a kinesin associated with a microtubule at position (n+1, toward the plus end) (5.6.1.3)
Summaries
Gene Snapshot
Kinesin-like protein at 3A (Klp3A) encodes a microtubule motor protein involved in chromosome segregation and mitotic spindle morphogenesis. [Date last reviewed: 2019-07-11]
Gene Group (FlyBase)
KINESINS -
Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
mei-352 (R.S. Hawley)
mei-352 females exhibit near normal levels of meiotic exchange but the distribution of crossover events is abnormal (i.e., exchanges are more uniformly distributed per unit length than they are in wild-type). This relaxation of the normal constraints on the distribution of euchromatic exchange is clearly demonstrated by the ability of mei-352 females to allow exchange between the normally achiasmate fourth chromosomes (Sandler and Szauter, 1978). mei-352 does not, however, permit exchanges to occur in heterochromatic intervals (Carpenter and Baker, 1982). It remains to be demonstrated that the reduced fertility and the exchange phenotype are the consequence of the same mutational lesion. Although no increase in spontaneous chromosome aberrations was observed upon direct cytological examination of larval neuroblasts (Gatti, 1979), the mei-352 mutation was shown to increase mitotic chromosome instability approximately threefold when assayed genetically (Baker et al., 1978). This suggests that the mei-352+ gene product is required both during meiosis and mitosis.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Klp3A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9XZ29)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Klp3A-RA
FBtr0070448
NM_080314
4527
1212
Klp3A-RB
FBtr0340093
NM_001297901
4090
1212
Additional Transcript Data and Comments
Reported size (kB)

4.4 (unknown)

(Williams et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Klp3A-PA
FBpp0070432
135.8
1212
7.00
Q9XZ29
NP_525053
AAF45793
Klp3A-PB
FBpp0309092
135.8
1212
7.00
Q9XZ29
NP_001284830
AHN59301
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1212 aa isoforms: Klp3A-PA, Klp3A-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

140 (kD observed); 138 (kD predicted)

(Williams et al., 1995)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp3A using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.07

Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Williams et al., 1997)
oogenesis
(Williams et al., 1997)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Dorus et al., 2006)
Additional Descriptive Data

During the anaphase/telophase of female meiosis, Klp3A protein localizes to the spindle equator, in the midbody. Unlike its diffuse distribution in male meiosis, Klp3A protein is located in the spindle during metaphase, both in female meiotic cells and in syncytial blastoderm nuclei. Klp3A protein is also detected in the sperm aster and in the radial, monastral array of microtubules established between the two meiosis II spindles ("middle pole").

(Williams et al., 1997)

During late anaphase and telophase of male meiosis, Klp3A protein localizes to the equator of the central spindle.

(Williams et al., 1995)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in aster
inferred from direct assay
(Williams et al., 1997)
located_in cytoplasm
inferred from direct assay
(Williams et al., 1995)
part_of microtubule associated complex
inferred from direct assay
(Williams et al., 1997)
located_in midbody
inferred from direct assay
(Kwon et al., 2004)
located_in nucleus
inferred from direct assay
(Kwon et al., 2004, Williams et al., 1995)
located_in spindle midzone
inferred from direct assay
(Kwon et al., 2004)
located_in spindle
inferred from direct assay
(Williams et al., 1997, Williams et al., 1995)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Klp3A in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Klp3A
Transgenic constructs containing regulatory region of Klp3A
Aberrations (Deficiencies and Duplications) ( 20 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | maternal effect
lethal - all die during embryonic stage | maternal effect
lethal - all die during embryonic stage | maternal effect (with Df(1)ED6579)
lethal - all die during embryonic stage | maternal effect (with Klp3A1)
lethal - all die during embryonic stage | maternal effect (with Klp3A2)
lethal - all die during embryonic stage | maternal effect (with Klp3A6)
lethal - all die during embryonic stage | maternal effect (with Klp3A63e4)
lethal - all die during embryonic stage | maternal effect (with Klp3Amei-352)
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female semi-sterile
female semi-sterile (with Klp3A2)
female semi-sterile (with Klp3A63e4)
female semi-sterile (with Klp3Amei-352)
female sterile
female sterile (with Df(1)ED6579)
female sterile (with Klp3A1)
female sterile (with Klp3A6)
female sterile (with Klp3Amei-352)
male semi-sterile
male sterile
Other Phenotypes
Allele
abnormal meiotic cell cycle
abnormal meiotic cell cycle (with Df(1)ED6579)
abnormal meiotic cell cycle | recessive
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
some die during embryonic stage | maternal effect
some die during embryonic stage | maternal effect (with Df(1)ED6579)
some die during embryonic stage | maternal effect (with Klp3A1)
some die during embryonic stage | maternal effect (with Klp3A2)
some die during embryonic stage | maternal effect (with Klp3A6)
some die during embryonic stage | maternal effect (with Klp3A63e4)
some die during embryonic stage | maternal effect (with Klp3Amei-352)
wild-type
Phenotype manifest in
Allele
contractile ring & meiotic cell cycle | male
meiotic cell cycle
Nebenkern
spermatid
spermatocyte ring canal
spindle & meiotic cell cycle | male
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (37)
Hsap\KIF4A
12 of 14
Yes
Yes
Hsap\KIF4B
11 of 14
No
Yes
Hsap\KIF21A
4 of 14
No
No
Hsap\KIF21B
4 of 14
No
No
1  
Hsap\KIF7
3 of 14
No
Yes
1  
Hsap\KIF27
3 of 14
No
Yes
Hsap\CENPE
1 of 14
No
No
Hsap\KIF1A
1 of 14
No
No
Hsap\KIF1B
1 of 14
No
No
Hsap\KIF1C
1 of 14
No
No
Hsap\KIF2A
1 of 14
No
No
Hsap\KIF2B
1 of 14
No
No
Hsap\KIF2C
1 of 14
No
No
Hsap\KIF3A
1 of 14
No
No
1  
Hsap\KIF3B
1 of 14
No
No
Hsap\KIF3C
1 of 14
No
No
Hsap\KIF5A
1 of 14
No
No
5  
Hsap\KIF5B
1 of 14
No
No
1  
Hsap\KIF5C
1 of 14
No
No
Hsap\KIF11
1 of 14
No
No
1  
Hsap\KIF13A
1 of 14
No
No
Hsap\KIF13B
1 of 14
No
No
Hsap\KIF14
1 of 14
No
No
Hsap\KIF15
1 of 14
No
No
Hsap\KIF16B
1 of 14
No
No
Hsap\KIF17
1 of 14
No
No
Hsap\KIF18A
1 of 14
No
No
Hsap\KIF18B
1 of 14
No
No
Hsap\KIF19
1 of 14
No
No
Hsap\KIF20A
1 of 14
No
No
Hsap\KIF20B
1 of 14
No
No
Hsap\KIF22
1 of 14
No
No
Hsap\KIF23
1 of 14
No
No
Hsap\KIF24
1 of 14
No
No
Hsap\KIFC1
1 of 14
No
No
Hsap\KIFC2
1 of 14
No
No
Hsap\KIFC3
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (30)
Rnor\Kif4a
12 of 14
Yes
Yes
Rnor\Kif21b
4 of 14
No
No
Rnor\Kif7
3 of 14
No
Yes
Rnor\Kif21a
3 of 14
No
No
Rnor\Kif27
3 of 14
No
Yes
Rnor\Cenpe
1 of 14
No
No
Rnor\Kif2a
1 of 14
No
No
Rnor\Kif2c
1 of 14
No
No
Rnor\Kif3a
1 of 14
No
No
Rnor\Kif3b
1 of 14
No
No
Rnor\Kif3c
1 of 14
No
No
Rnor\Kif4b
1 of 14
No
Yes
Rnor\Kif5a
1 of 14
No
No
Rnor\Kif5b
1 of 14
No
No
Rnor\Kif5c
1 of 14
No
No
Rnor\Kif11
1 of 14
No
No
Rnor\Kif12
1 of 14
No
No
Rnor\Kif13b
1 of 14
No
No
Rnor\Kif14
1 of 14
No
No
Rnor\Kif15
1 of 14
No
No
Rnor\Kif16b
1 of 14
No
No
Rnor\Kif17
1 of 14
No
No
Rnor\Kif18b
1 of 14
No
No
Rnor\Kif19
1 of 14
No
No
Rnor\Kif20a
1 of 14
No
No
Rnor\Kif20b
1 of 14
No
No
Rnor\Kif22
1 of 14
No
No
Rnor\Kif23
1 of 14
No
No
Rnor\Kifc1
1 of 14
No
No
Rnor\Kifc3
1 of 14
No
No
Mus musculus (laboratory mouse) (32)
Mmus\Kif4
12 of 14
Yes
Yes
Mmus\Kif21a
4 of 14
No
No
1  
Mmus\Kif7
3 of 14
No
Yes
Mmus\Kif21b
3 of 14
No
No
Mmus\Kif27
3 of 14
No
Yes
Mmus\Cenpe
1 of 14
No
No
Mmus\Kif1a
1 of 14
No
No
Mmus\Kif2a
1 of 14
No
No
Mmus\Kif3a
1 of 14
No
No
1  
Mmus\Kif3b
1 of 14
No
No
Mmus\Kif3c
1 of 14
No
No
Mmus\Kif5a
1 of 14
No
No
Mmus\Kif5b
1 of 14
No
No
Mmus\Kif5c
1 of 14
No
No
Mmus\Kif11
1 of 14
No
No
Mmus\Kif13a
1 of 14
No
No
Mmus\Kif13b
1 of 14
No
No
Mmus\Kif14
1 of 14
No
No
Mmus\Kif15
1 of 14
No
No
Mmus\Kif16b
1 of 14
No
No
Mmus\Kif17
1 of 14
No
No
Mmus\Kif18a
1 of 14
No
No
Mmus\Kif18b
1 of 14
No
No
Mmus\Kif19a
1 of 14
No
No
Mmus\Kif19b
1 of 14
No
No
Mmus\Kif20a
1 of 14
No
No
Mmus\Kif20b
1 of 14
No
No
Mmus\Kif22
1 of 14
No
No
Mmus\Kif23
1 of 14
No
No
Mmus\Kifc1
1 of 14
No
No
Mmus\Kifc3
1 of 14
No
No
Mmus\Kifc5b
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (22)
Xtro\kif4a
8 of 13
Yes
Yes
Xtro\kif27
2 of 13
No
Yes
Xtro\LOC100494653
2 of 13
No
Yes
Xtro\dcun1d5
1 of 13
No
No
Xtro\kif5b
1 of 13
No
No
Xtro\kif5c
1 of 13
No
No
Xtro\kif11
1 of 13
No
No
Xtro\kif13a
1 of 13
No
No
Xtro\kif13b
1 of 13
No
No
Xtro\kif14
1 of 13
No
No
Xtro\kif18a
1 of 13
No
No
Xtro\kif18b
1 of 13
No
No
Xtro\kif20a
1 of 13
No
No
Xtro\kif20b
1 of 13
No
No
Xtro\kif21a
1 of 13
No
No
Xtro\kif21b
1 of 13
No
No
Xtro\kif22
1 of 13
No
No
Xtro\kif23
1 of 13
No
No
Xtro\kifc1
1 of 13
No
No
Xtro\kifc2
1 of 13
No
No
Xtro\shisa7
1 of 13
No
Yes
Xtro\stard9
1 of 13
No
No
Danio rerio (Zebrafish) (36)
Drer\kif4
12 of 14
Yes
Yes
Drer\kif7
3 of 14
No
Yes
Drer\kif21a
3 of 14
No
No
Drer\kif21b
3 of 14
No
No
Drer\kif1aa
1 of 14
No
No
Drer\kif1ab
1 of 14
No
No
Drer\kif1b
1 of 14
No
No
Drer\kif3a
1 of 14
No
No
Drer\kif3b
1 of 14
No
No
Drer\kif3cb
1 of 14
No
No
Drer\kif3c
1 of 14
No
No
Drer\kif5aa
1 of 14
No
No
Drer\kif5ab
1 of 14
No
No
Drer\kif5ba
1 of 14
No
No
Drer\kif5c
1 of 14
No
No
Drer\kif11
1 of 14
No
No
Drer\kif13a
1 of 14
No
No
Drer\kif13ba
1 of 14
No
No
Drer\kif13bb
1 of 14
No
No
Drer\kif14
1 of 14
No
No
Drer\kif15
1 of 14
No
No
Drer\kif17
1 of 14
No
No
Drer\kif18a
1 of 14
No
No
Drer\kif19
1 of 14
No
No
Drer\kif20a
1 of 14
No
No
Drer\kif20bb
1 of 14
No
No
Drer\kif22
1 of 14
No
No
Drer\kif23
1 of 14
No
No
Drer\kifc1
1 of 14
No
No
Drer\kifc3
1 of 14
No
No
Drer\si:cabz01066312.1
1 of 14
No
No
Drer\si:ch211-257p13.3
1 of 14
No
No
Drer\si:dkey-26i13.8
1 of 14
No
No
Drer\si:dkey-96l17.6
1 of 14
No
No
Drer\stard9
1 of 14
No
No
Drer\zgc:56231
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (18)
Cele\klp-19
12 of 14
Yes
Yes
Cele\klp-12
3 of 14
No
No
Cele\bmk-1
1 of 14
No
No
Cele\klp-3
1 of 14
No
No
Cele\klp-4
1 of 14
No
No
Cele\klp-6
1 of 14
No
No
Cele\klp-7
1 of 14
No
No
Cele\klp-11
1 of 14
No
No
Cele\klp-13
1 of 14
No
No
Cele\klp-15
1 of 14
No
No
Cele\klp-16
1 of 14
No
No
Cele\klp-17
1 of 14
No
No
Cele\klp-18
1 of 14
No
No
Cele\klp-20
1 of 14
No
No
Cele\osm-3
1 of 14
No
No
Cele\unc-104
1 of 14
No
No
Cele\unc-116
1 of 14
No
No
Cele\zen-4
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (19)
Agam\AgaP_AGAP000575
11 of 12
Yes
Yes
Agam\AgaP_AGAP012471
9 of 12
No
Yes
Agam\AgaP_AGAP007815
3 of 12
No
No
Agam\AgaP_AGAP000159
1 of 12
No
No
Agam\AgaP_AGAP000561
1 of 12
No
No
Agam\AgaP_AGAP002248
1 of 12
No
No
Agam\AgaP_AGAP002427
1 of 12
No
No
Agam\AgaP_AGAP003323
1 of 12
No
No
Agam\AgaP_AGAP003925
1 of 12
No
No
Agam\AgaP_AGAP006472
1 of 12
No
No
Agam\AgaP_AGAP006803
1 of 12
No
No
Agam\AgaP_AGAP006989
1 of 12
No
No
Agam\AgaP_AGAP007502
1 of 12
No
No
Agam\AgaP_AGAP007592
1 of 12
No
No
Agam\AgaP_AGAP007706
1 of 12
No
No
Agam\AgaP_AGAP008035
1 of 12
No
No
Agam\AgaP_AGAP010396
1 of 12
No
No
Agam\AgaP_AGAP011874
1 of 12
No
No
Agam\KIF1A_ANOGA
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (56)
Atha\AT5G60930
12 of 13
Yes
Yes
Atha\KICP-02
11 of 13
No
Yes
Atha\FRA1
10 of 13
No
Yes
Atha\AT5G33300
2 of 13
No
Yes
Atha\ARK2
1 of 13
No
No
Atha\ARK3
1 of 13
No
No
Atha\AT1G09170
1 of 13
No
No
Atha\AT1G18410
1 of 13
No
No
Atha\AT1G18550
1 of 13
No
No
Atha\AT1G21730
1 of 13
No
No
Atha\AT1G55550
1 of 13
No
No
Atha\AT1G63640
1 of 13
No
No
Atha\AT1G72250
1 of 13
No
No
Atha\AT1G73860
1 of 13
No
No
Atha\AT2G21380
1 of 13
No
No
Atha\AT2G22610
1 of 13
No
No
Atha\AT2G28620
1 of 13
No
No
Atha\AT2G36200
1 of 13
No
No
Atha\AT2G37420
1 of 13
No
No
Atha\AT2G47500
1 of 13
No
No
Atha\AT3G10180
1 of 13
No
No
Atha\AT3G10310
1 of 13
No
No
Atha\AT3G12020
1 of 13
No
No
Atha\AT3G16060
1 of 13
No
No
Atha\AT3G44050
1 of 13
No
No
Atha\AT3G45850
1 of 13
No
No
Atha\AT3G49650
1 of 13
No
No
Atha\AT3G63480
1 of 13
No
No
Atha\AT4G14330
1 of 13
No
No
Atha\AT4G24170
1 of 13
No
No
Atha\AT4G39050
1 of 13
No
No
Atha\AT5G02370
1 of 13
No
No
Atha\AT5G06670
1 of 13
No
No
Atha\AT5G23910
1 of 13
No
Yes
Atha\AT5G27550
1 of 13
No
No
Atha\AT5G27950
1 of 13
No
No
Atha\AT5G41310
1 of 13
No
No
Atha\AT5G66310
1 of 13
No
No
Atha\ATK1
1 of 13
No
No
Atha\ATK2
1 of 13
No
No
Atha\ATK3
1 of 13
No
No
Atha\ATK4
1 of 13
No
No
Atha\ATK5
1 of 13
No
No
Atha\HIK
1 of 13
No
No
Atha\KAC1
1 of 13
No
No
Atha\KAC2
1 of 13
No
No
Atha\KINESIN-12B
1 of 13
No
No
Atha\KINESIN-13A
1 of 13
No
No
Atha\KP1
1 of 13
No
No
Atha\MRH2
1 of 13
No
No
Atha\PAKRP1
1 of 13
No
No
Atha\POK1
1 of 13
No
No
Atha\POK2
1 of 13
No
No
Atha\TES
1 of 13
No
No
Atha\ZCF125
1 of 13
No
No
Atha\ZWI
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (4)
Scer\KAR3
1 of 13
Yes
No
Scer\KIP1
1 of 13
Yes
No
Scer\KIP2
1 of 13
Yes
No
Scer\KIP3
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (7)
Spom\klp2
1 of 12
Yes
No
Spom\klp3
1 of 12
Yes
No
Spom\klp5
1 of 12
Yes
No
Spom\klp6
1 of 12
Yes
No
Spom\klp9
1 of 12
Yes
No
Spom\pkl1
1 of 12
Yes
No
Spom\tea2
1 of 12
Yes
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Klp3A. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (25)
Klp31E
5 of 13
nod
3 of 13
cmet
2 of 13
Khc-73
2 of 13
Khc
2 of 13
Kif3C
2 of 13
Kif19A
2 of 13
Klp10A
2 of 13
Klp54D
2 of 13
Klp59C
2 of 13
Klp59D
2 of 13
Klp61F
2 of 13
Klp64D
2 of 13
Klp67A
2 of 13
Klp68D
2 of 13
Klp98A
2 of 13
ncd
2 of 13
pav
2 of 13
sub
2 of 13
unc-104
2 of 13
cana
1 of 13
CG5004
1 of 13
CG32318
1 of 13
cos
1 of 13
neb
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    Klp3AGLC01416
    model of  epilepsy
    CEA
    (Liu et al., 2023)
    Potential Models Based on Orthology ( 3 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    KIF4A; kinesin family member 4A
    12 of 14
    300521
    KIF4B; kinesin family member 4B
    11 of 14
    609184
        KIF21A; kinesin family member 21A
        4 of 14
        608283
        KIF21B; kinesin family member 21B
        4 of 14
        608322
             
            KIF7; kinesin family member 7
            3 of 14
            611254
             
            KIF27; kinesin family member 27
            3 of 14
            611253
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Interactions
                Summary of Physical Interactions
                Interaction Browsers
                View in FlyBase Interactions Browser   View in MIST tool (external link)
                Please see the Physical Interaction reports below for full details
                protein-protein
                Physical Interaction
                Assay
                References
                Klp3A - feo
                anti tag coimmunoprecipitation, western blot, peptide massfingerprinting
                (D'Avino et al., 2007)
                Summary of Genetic Interactions
                Interaction Browsers
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                External Data
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                DroID - A comprehensive database of gene and protein interactions.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                FlyBase
                External Links
                External Data
                Linkouts
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Class of Gene
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                X
                Recombination map
                1-1
                Cytogenetic map
                3A6-3A6
                Sequence location
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                3A6-3A6
                Limits computationally determined from genome sequence between P{EP}eghEP804 and P{EP}sggEP1576
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                3A-3A
                (Page et al., 2004, William, 1993)
                3A5-3A8
                (determined by in situ hybridisation)
                (Williams et al., 1995)
                Mapped by classical genetic and deficiency mapping.
                (Page et al., 2004)
                Experimentally Determined Recombination Data
                Location

                1-1

                (FlyBase, 2016)

                1-1.0

                (Comeron et al., 2012)

                1-0.0

                Left of (cM)
                Right of (cM)
                Notes
                Stocks and Reagents
                Stocks (12)
                Genomic Clones (13)
                 

                Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                cDNA Clones (33)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                  Drosophila Genomics Resource Center cDNA clones

                  For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                  cDNA Clones, End Sequenced (ESTs)
                  BDGP DGC clones
                  Other clones
                  RNAi and Array Information
                  Linkouts
                  DRSC - Results frm RNAi screens
                  Antibody Information
                  Laboratory Generated Antibodies
                   

                  polyclonal

                  (Williams et al., 1995)
                  Commercially Available Antibodies
                   
                  Cell Line Information
                  Publicly Available Cell Lines
                   
                    Other Stable Cell Lines
                     
                      Other Comments

                      RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

                      (Goshima et al., 2007)

                      Mutations in Klp3A alter the distribution of meiotic exchanges without greatly affecting their total frequency.

                      (Page and Hawley, 2005)

                      Klp3A is required to establish and maintain the correct spacing between daughter nuclei during mitosis in embryos.

                      (Kwon et al., 2004)

                      dsRNA made from templates generated with primers directed against this gene in S2 cells causes defects in spindle morphology and dynamics. Spindles are shorter than in wild type and spindle microtubules are highly disorganized. Additionally, the mitotic index of prometaphase and metaphase figures is higher than wild type.

                      (Kwon et al., 2004)

                      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                      (Kiger et al., 2003)

                      Klp3A is necessary for chromosome segregation and can interact specifically with the centromere. Klp3A requires only centromeric sequences for its action in chromosome segregation.

                      (Cook et al., 1997)

                      The major defect caused by depletion of the Klp3A protein is either in specification of the female pronucleus, or in migration of the male and female pronuclei toward each other.

                      (Williams et al., 1997)

                      Mutations have a dramatic effect on the initiation of cytokinesis in testes probably due to the disruption of microtubule interdigitation in spermatocyte central spindles. Anaphase B spindle elongation is not affected. Klp3A function is important in the establishment or stabilisation of the central spindle and this structure is the source of signals that initiate the cleavage furrow.

                      (Williams et al., 1995)

                      Klp3A function is not essential to viability but is required for fertility in both sexes. The establishment of the prominent central spindle and midbody at late anaphase/telophase requires normal Klp3A activity.

                      (Williams et al., 1995)

                      The sequence of the Klp3A protein has been compared with the sequences of a variety of kinesin family proteins.

                      (Goodson et al., 1994)

                      The Klp3A+ gene product is required both during meiosis and mitosis.

                      Relationship to Other Genes
                      Source for database merge of

                      Source for merge of: EG:BACR25B3.9 Klp3A

                      (Bayraktaroglu, 2000.8.7)

                      Source for merge of: Klp3A mei-352

                      (Page, 2004.3.5)

                      Source for merge of: Klp3A BcDNA:LD21815

                      (FlyBase, 1996-, Williams et al., 1995)
                      Additional comments

                      Experiments strongly suggest that "Klp3A" is the gene responsible for the "mei-352" mutant; Klp3A1 and Klp3A63e4 fail to complement the maternal-effect lethal phenotype of Klp3Amei-352, embryos from Klp3Amei-352/Klp3A1 transheterozygous females have a pronuclear fusion defect identical to the phenotype observed for Klp3A homozygotes and the Klp3Amei-352-bearing chromosome has a glutamic acid to lysine missense mutation in the kinesin-like motor domain of Klp3A that is not present on the parental chromosome.

                      (Page et al., 2004)

                      Source for merge of Klp3A BcDNA:LD21815 was sequence comparison ( date:990717 ).

                      (FlyBase, 1996-, Williams et al., 1995)
                      Nomenclature History
                      Source for database identify of
                      Nomenclature comments
                      Etymology
                      Synonyms and Secondary IDs (23)
                      Reported As
                      Symbol Synonym
                      3A7Kin
                      (Goodson et al., 1994)
                      BcDNA:LD21815
                      CG8590
                      (Çiçek et al., 2016, Moutinho-Pereira et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ducat et al., 2008, Hughes et al., 2008, Goshima et al., 2007, Benos, 2000.12.13, Goldstein and Gunawardena, 2000)
                      DmKLP3A
                      (Hirokawa et al., 1998)
                      DmKlp3A
                      (Hirokawa, 2012, Dagenbach and Endow, 2004, Kim and Endow, 2000, Moore and Endow, 1996)
                      EG:BACR25B3.9
                      (Benos et al., 2001, Benos, 2000.12.13)
                      KIF 4A
                      (Miki et al., 2001)
                      KIF4
                      (D'Avino et al., 2005)
                      KIF4A
                      (Hirokawa, 2012.11.20)
                      KLP-3A
                      (Field et al., 1999)
                      KLP3A
                      (Wang et al., 2015, Cane et al., 2013, Giansanti et al., 2012, Civelekoglu-Scholey et al., 2010, Vendra et al., 2007, Silverman-Gavrila and Wilde, 2006, Jang et al., 2005, Miki et al., 2005, Giansanti et al., 2004, Kwon and Scholey, 2004, Kwon et al., 2004, Lawrence et al., 2002, Loppin et al., 2000, Sisson et al., 2000, Straight and Field, 2000, Giansanti et al., 1999, Herrmann et al., 1999, Bonaccorsi et al., 1998, Giansanti et al., 1998, Lane and Allan, 1998)
                      Klp 3A
                      (Miki et al., 2001)
                      Klp3A
                      (Qu et al., 2026, Vesala et al., 2024, Curnutte et al., 2023, Khalili et al., 2023, Deshpande et al., 2022, Deng et al., 2021, Deshpande and Telley, 2021, Lv et al., 2021, Witte et al., 2020, Zheng et al., 2020, Costa and Ohkura, 2019, Fingerhut et al., 2019, Verma and Maresca, 2019, Sarov et al., 2016, Brust-Mascher and Scholey, 2015.2.12, Nikalayevich and Ohkura, 2015, Hughes et al., 2014, Liang et al., 2014, Kwon et al., 2013, Moutinho-Pereira et al., 2013, Antao et al., 2012, Giansanti et al., 2012, Hirokawa, 2012.11.20, Japanese National Institute of Genetics, 2012.5.21, Reis et al., 2011, Raja et al., 2010, Wasbrough et al., 2010, Anderson et al., 2009, Ducat et al., 2008, Fisher et al., 2008, Somma et al., 2008, D'Avino et al., 2007, Georlette et al., 2007, Goshima et al., 2007, Dorus et al., 2006, Dorus et al., 2006, Mukai et al., 2006)
                      Klp3a
                      (Vicars et al., 2025, Valsecchi et al., 2018, Chlamydas et al., 2016, Conrad et al., 2012, Mazumdar and Mazumdar, 2002, Miki et al., 2001)
                      fs(1)M4
                      (Williams et al., 1995, Voelker et al., 1989)
                      klp3A
                      (Kitazawa et al., 2014, Giansanti et al., 2012, Lesch and Page, 2012, D'Avino et al., 2005, Gatt et al., 2005, Page et al., 2004, Somma et al., 2002, Cook and Karpen, 1997, Cook et al., 1997, William, 1993)
                      klp3a
                      (Karg et al., 2017, Hallacli et al., 2012)
                      mei-352
                      (Deng et al., 2021, Page and Hawley, 2005, Hawley, 1993, Hawley et al., 1993, Boyd and Shaw, 1982, Carpenter and Baker, 1982, Mason et al., 1981, Gatti, 1979, Baker and Carpenter, 1972)
                      Name Synonyms
                      Kinesin-Like-Protein-at-3A
                      (Williams et al., 1995)
                      Kinesin-like protein at 3A
                      (Hirokawa, 2012.11.20, Wasbrough et al., 2010, Mukai et al., 2006)
                      Kinesin5
                      (Kollmar, 2015.9.15)
                      female sterile(1)M4
                      meiotic 352
                      Secondary FlyBase IDs
                      • FBgn0000827
                      • FBgn0002711
                      • FBgn0010237
                      • FBgn0040374
                      Datasets (0)
                      Study focus (0)
                      Experimental Role
                      Project
                      Project Type
                      Title
                      Study result (0)
                      Result
                      Result Type
                      Title
                      External Crossreferences and Linkouts ( 46 )
                      Sequence Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                      Other crossreferences
                      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                      FlyMine - An integrated database for Drosophila genomics
                      InterPro - A database of protein families, domains and functional sites
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      MARRVEL_MODEL - MARRVEL (model organism gene)
                      Linkouts
                      BioGRID - A database of protein and genetic interactions.
                      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                      DroID - A comprehensive database of gene and protein interactions.
                      DRSC - Results frm RNAi screens
                      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                      Flygut - An atlas of the Drosophila adult midgut
                      FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                      MIST (protein-protein) - An integrated Molecular Interaction Database
                      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                      References (169)