Gene Dmel\Med

(Wisotzkey et al., 1998, Decotto and Ferguson, 2001)

Right of (cM)

l(3)00720

Notes

Also mapped to 3-49.3 based on mapping of l(3)SG36, in error.

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: Med+ anon-H31? anon-I7114? awd? ttk?

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Med for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Med-RA

FBtr0085815

NM_079871

3804

771

Med-RB

FBtr0085816

NM_170559

3582

697

Additional Transcript Data & Comments

Reported size (kB)

3.1-3.3 (northern blot)

3.3 (longest cDNA)

3.03 (longest cDNA)

(Xu et al., 1998)

2.8 (longest cDNA)

(Wisotzkey et al., 1998, Das et al., 1998, Hudson et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Med-PA

FBpp0085176

81.6

771

7.60

NP_524610

AAF57172

Med-PB

FBpp0085177

73.7

697

7.45

NP_733438

AAN14277

Additional Polypeptide Data & Comments

Reported size (kD)

771 (aa)

697 (aa)

(Xu et al., 1998)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0011655

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

SMAD domain, Dwarfin-type (IPR001132)

MAD homology 1, Dwarfin-type (IPR003619)

SMAD/FHA domain (IPR008984)

MAD homology, MH1 (IPR013019)

Dwarfin (IPR013790)

SMAD domain-like (IPR017855)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

DYR16-72

(BDGP Project Members, 2000-)

Does NOT map to

Identified with

LD07433

(Inoue et al., 1998)

LD22279

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

deletion

Med[11]
3R:27,438,831..27,439,590

comment=The Med[11] deletion is annotated as including an entire intron and the adjacent exon; the 405bp deletion actually extends from the 3' end of the intron into the exon; the exact breakpoints were not reported.
evidence=experimental

point mutation

Med[13]
3R:27,437,832..27,437,832

evidence=experimental
na_change=C27437832T
pr_change=R66|Med-PA,R66|Med-PB
reported_na_change=C814T
reported_pr_change=?66@

(Xu et al., 1998)

point mutation

Med[7]
3R:27,437,931..27,437,932

comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=T27437931A
pr_change=C99S|Med-PA,C99S|Med-PB
reported_pr_change=C99S

point mutation

Med[1]
3R:27,438,767..27,438,767

comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=C27438767T
pr_change=Q283@|Med-PA
reported_pr_change=Q283@

point mutation

Med[16]
3R:27,439,257..27,439,257

comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=C27439257T
pr_change=Q396|Med-PA,Q322|Med-PB
reported_pr_change=Q370@

point mutation

Med[C246]
3R:27,439,265..27,439,265

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Med-PB is only CDS with Y at position 324.
evidence=experimental
na_change=T27439265G
pr_change=Y398|Med-PA,Y324|Med-PB
reported_pr_change=Y324@

(McCabe et al., 2004)

point mutation

Med[23]
3R:27,439,440..27,439,440

na_change=C27439440T
comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
pr_change=Q457|Med-PA,Q383|Med-PB
reported_pr_change=Q457@

point mutation

Med[K465]
3R:27,439,597..27,439,598

comment=Position of mutation on reference sequence inferred by FlyBase curator based on author statement. Mutation in the splice donor site of exon 4. Exon 4 of Med-PB used because authors appear to be referring to this transcript (see Med[C246])
evidence=experimental

(McCabe et al., 2004)

point mutation

Med[G112]
3R:27,439,597..27,439,598

(McCabe et al., 2004)

point mutation

Med[14]
3R:27,439,657..27,439,658

comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
pr_change=W435|Med-PB,W509|Med-PA,W409|Med-PD, W483|M ed-PC
reported_pr_change=W483@

point mutation

Med[26]
3R:27,440,017..27,440,017

comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=G27440017A
pr_change=G609S|Med-PA,G535S|Med-PB
reported_pr_change=G609S

point mutation

Med[21]
3R:27,440,018..27,440,018

comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=G27440018C
pr_change=G609A|Med-PA,G535A|Med-PB
reported_pr_change=G609A

point mutation

Med[12]
3R:27,440,054..27,440,055

comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
pr_change=W547|Med-PB,W621|Med-PA,W521|Med-PD, W595|M ed-PC
reported_pr_change=W595@

point mutation

Med[15]-1
3R:27,440,421..27,440,421

comment=One of two amino acid changes reported in Med[15] mutant.
evidence=experimental
pr_change=A631V|Med-PB,A705V|Med-PA,A605V|Med-PD, A679V|M ed-PC
reported_pr_change=A679V

point mutation

Med[19]
3R:27,440,442..27,440,442

comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=A27440442C
pr_change=D712A|Med-PA,D638A|Med-PB
reported_pr_change=D712A

point mutation

Med[6]
3R:27,440,487..27,440,487

comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=G27440487A
pr_change=G727D|Med-PA,G653D|Med-PB
reported_pr_change=G727D

point mutation

Med[8]
3R:27,440,490..27,440,491

comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
pr_change=W654|Med-PB,W728|Med-PA,W628|Med-PD, W702|M ed-PC
reported_pr_change=W728@

point mutation

Med[15]-2
3R:27,440,526..27,440,526

comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=C27440526A
pr_change=T740K|Med-PA,T666K|Med-PB
reported_pr_change=T714K

point mutation

Med[17]
3R:27,440,528..27,440,528

evidence=experimental
na_change=C27440528A
pr_change=P741T|Med-PA,P667T|Med-PB
reported_pr_change=P715T
comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change.

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

11516

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 16

midgut constriction 2

embryonic stage | stage 14

ectoderm

embryonic stage | stage 7

mesoderm

embryonic stage | stage 7

embryonic stage | stage 14

endoderm

embryonic stage | stage 4

embryonic stage | >=stage 14

embryonic central nervous system | restricted