l(3)SG70, dSmad4, l(3)11m-254
Smad family member - associates with Smad1 in response to Dpp or with Smad2 (Smox) in response to Activin ligands - dominant negative Smad4 blocks both BMP and activin responses - developmental roles include dorsal-ventral patterning, patterning and proliferation of the wing disc and gene expression in the mushroom body of the larval brain
Please see the JBrowse view of Dmel\Med for information on other features
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Gene model reviewed during 5.48
Gene model reviewed during 5.46
Gene model reviewed during 5.53
2.8 (longest cDNA)
3.1-3.3 (northern blot)
3.3 (longest cDNA)
3.03 (longest cDNA)
697 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Med using the Feature Mapper tool.
Comment: maternally deposited
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Med transcript expression is widespread in the stage 4 embryo. At stage 7, expression is detected in the mesoderm and in the anterior embryo. At stage 14, expression is detected in the ectoderm, endoderm, and the central nervous system. At stage 16, the CNS, the second midgut constriction, as well as other tissues, accumulate Med transcript.
Med protein is localized at oogenesis stage S9 to anterior follicle cells that migrate towards the oocyte, and at stage S10 to all follicle cells, with stronger expression observed in stretch cells.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Med in GBrowse 23-102
3-102
3-99.1
3-130.2
3-106
Also mapped to 3-49.3 based on mapping of l(3)SG36, in error.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Med CG1775
Source for merge of: Med E(zen)3
Source for merge of: Med anon- EST:Posey121
DNA-protein interactions: genome-wide binding profile assayed for Med protein in 2-3 hr embryos; see BDTNP1_TFBS_Med collection report.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Overexpression of Med or Hsap\MADH4 in the wing and leg causes a similar phenotype. The leg phenotype caused by overexpression of Med or Hsap\MADH4 is similar to that caused by overexpression of Hsap\MADH6 or Hsap\MADH7. Overexpression of Med, Smox, Hsap\MADH2 or Hsap\MADH4 causes a similar phenotype in the wing.
Hsap\MADH1 and Hsap\MADH4, as well as Mad and Med, can stimulate dpp signalling in limb development. Hsap\MADH2, Hsap\MADH4 and Med stimulate activin-β- rather than dpp-mediated cell proliferation. Med can signal for both TGF-beta families - Dpp/BMP signalling Smads and TGF-beta/Activin Smads.
Mutants show no interaction with Df(2R)Pcl11B or Df(3L)66C-G28.
Dominant enhancer of zen.