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Gene Dmel\msi

General Information
Symbol	Dmel\msi	Species	D. melanogaster
Name	musashi	Annotation symbol	CG5099
Feature type	protein_coding_gene	FlyBase ID	FBgn0011666
Gene Model Status	Current	Stock availability	23 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	96E2-96E4	Sequence location	3R:21,341,840..21,434,145 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene musashi is referred to in FlyBase by the symbol Dmel\msi (CG5099, FBgn0011666). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: RNA binding; mRNA binding. There is experimental evidence that it is involved in the biological process: chaeta development; stem cell maintenance; cellular response to hypoxia; germ-line stem cell division; negative regulation of translation. 25 alleles are reported. The phenotypes of these alleles are annotated with: rhabdomere; trichogen cell; testis sheath muscle; thecogen cell; testis; tormogen cell; ommatidium; socket; external sensory organ. It has 7 annotated transcripts and 7 annotated polypeptides. Protein features are: Nucleotide-binding, alpha-beta plait; RNA recognition motif domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1638125_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of msi. Gene sequence location is 3R:21341840..21434145.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96E2-96E4   Limits computationally determined from genome sequence between P{PZ}Aats-gln05461 and P{EP}EP3354
Experimentally Determined Cytological Location
Cytogenetic map Notes References 96E1-96E4     (Montell, 1994.5.19) 96E-96E   (determined by in situ hybridisation)   (Nakamura et al., 1994)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\msi for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated. Gene model reviewed during 5.47 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) msi-RA FBtr0084882  NM_079838   3984   606 msi-RB FBtr0084881  NM_170229   2806   634 msi-RC FBtr0305218  NM_001260374   2888   634 msi-RD FBtr0305219  NM_001260375   2801   605 msi-RE FBtr0333663  NM_001276030   2840   618 msi-RF FBtr0333664  NM_001276031   10175   634 msi-RH FBtr0333665  NM_001276032   2676   567
Additional Transcript Data & Comments
Reported size (kB)	4.2, 3.5, 3.0 (northern blot) (Nakamura et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein msi-PA   FBpp0084256   63.0   606   9.37     NP_524577   AAF56478 msi-PB   FBpp0084255   65.6   634   9.79     NP_733108   AAN14056 msi-PC   FBpp0293748   65.6   634   9.79     NP_001247303   AFH06620 msi-PD   FBpp0293749   62.5   605   9.73     NP_001247304   AFH06621 msi-PE   FBpp0305819   63.9   618   9.71     NP_001262959   AGB96339 msi-PF   FBpp0305820   65.6   634   9.79     NP_001262960   AGB96340 msi-PH   FBpp0305821   58.7   567   9.77     NP_001262961   AGB96341
Additional Polypeptide Data & Comments
Reported size (kDa)	606 (aa) (Nakamura et al., 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202227   AA440590   AA698049   AA951254   AE014297 AAF56478   AE014297 AAN14056   AF067892   AE014297 AFH06620   AE014297 AFH06621   AE014297 AGB96339   AE014297 AGB96340   AE014297 AGB96341   AI124317   AW943094   AY051802 AAK93226   AY119171 AAM51031   BG633463   BG638091   BI169922   BI173857   BI481609   BI588057   BI618573   BI631146   BT003274 AAO25031   BT100314   BT133366   CL610192   CO153059   CO154946   CO156026   CO156743   CO177636   CO186143   CO186373   CO187444   CO261880   CO263142   CO263596   CO264073   CO264728   CO265390   CO268510   CO268532   CO271237   CO272256   CO273117   CO273965   CO274179   CO274442   CO274485   CO274654   CO278162   CO279904   CO279915   CO281079   CO281811   CO282527   CO283056   CO284527   CO286421   CO288701   CO289263   CO294305   CO294556   CO294714   CO297727   CO300958   CO301504   CO301854   CO303776   CO305142   CO305706   CO305995   CO311118   CO311357   CO311624   CO313919   CO314785   CO315107   CO316186   CO316721   CO319884   CO320199   CO320548   CO322177   CO329346   CO329990   CO332028   CO333369   CO336613   CO340109   CO341171   CZ467336   CZ476403   CZ481832   CZ483441   CZ483442   CZ483571   CZ483572   CZ485958   CZ490401   DN154298   EC057370   EC060790   EC060793   EC061574   EC062107   EC062221   EC062662   EC063958   EC064046   EC064849   EC065809   EC066060   EC067407   EC070214   EC070732   EC070806   EC072096   EC072332   EC073131   EC073974   EC074029   EC077411   EC077444   EC078052   EC078818   EC081590   EC083348   EC088889   EC088890   EC090971   EC196876   EC198092   EC198152   EC198155   EC200516   EC201577   EC202175   EC202635   EC202678   EC202866   EC203873   EC204729   EC206184   EC206451   EC206967   EC208556   EC211610   EC214330   EC215937   EC216579   EC218457   EC219933   EC221694   EC224171   EC225281   EC229033   EC231225   EC233084   EC234247   EC236785   EC238550   EC239854   EC240834   EC240969   EC243959   EC245648   EC248613   EC248614   EC249584   EC256888   EC257260   EC261174   EC262875   EC264911   EC265986   EC266703   EV587180   EV591243   EV592580   GH771935   GH772491   GH778034   GH783430   GH784731   GH801848   GH803248   GH815391   GH828507   GH845874   GH860450   GH867760   GH879352   GH894074   GH907184   GH916193   GH919553   GH921116   GH927174   GH928495   GH934844   GH940440   GH940457   X79339 CAA55897   X79340 CAA55898   X79408
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IMS8 Q9VBQ1 Q8MS04 Q86PA1 Q7JN15
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Nakamura et al., 1994)
Expression Deduced from Reporters
Reporter: P{PZ}900 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval peripheral nervous system   (Nakamura et al., 1994) neuron of central nervous system   (Nakamura et al., 1994) larval stage   neuron of embryonic/larval nervous system   (Nakamura et al., 1994) pupal stage   sensory mother cell   (Nakamura et al., 1994) microchaeta   (Nakamura et al., 1994) macrochaeta   (Nakamura et al., 1994) adult stage   lamina   (Nakamura et al., 1994) adult brain   (Nakamura et al., 1994) antenna   (Nakamura et al., 1994)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    189.95 Larval Midgut    22.5 Larval Hindgut    15.2 Larval Malpighian Tubules    9 Larval Fat Body    11.3 Larval Salivary Gland    17.8 Larval Trachea    87.3 Larval Carcass    93.725 Adult Head    43.6 Adult Eye    82.95 Adult Brain    25.7 Adult Thoracic-Abdominal Ganglion    19.4 Adult Crop    96.4 Adult Midgut    14.4 Adult Hindgut    14.2 Adult Malpighian Tubules    4.1 Adult Fat Body    6.9 Adult Salivary Gland    10.6 Adult Heart    35.3 Adult VirginFemale Spermatheca    16.9 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    70.8 Adult Testis    12.7 Adult Male Accessory Gland    15 Adult Carcass    52.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    189.95 Larval Midgut    22.5 Larval Hindgut    15.2 Larval Malpighian Tubules    9 Larval Fat Body    11.3 Larval Salivary Gland    17.8 Larval Trachea    87.3 Larval Carcass    93.725 Adult Head    43.6 Adult Eye    82.95 Adult Brain    25.7 Adult Thoracic-Abdominal Ganglion    19.4 Adult Crop    96.4 Adult Midgut    14.4 Adult Hindgut    14.2 Adult Malpighian Tubules    4.1 Adult Fat Body    6.9 Adult Salivary Gland    10.6 Adult Heart    35.3 Adult VirginFemale Spermatheca    16.9 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    70.8 Adult Testis    12.7 Adult Male Accessory Gland    15 Adult Carcass    52.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    189.95 Larval Midgut    22.5 Larval Hindgut    15.2 Larval Malpighian Tubules    9 Larval Fat Body    11.3 Larval Salivary Gland    17.8 Larval Trachea    87.3 Larval Carcass    93.725 Adult Head    43.6 Adult Eye    82.95 Adult Brain    25.7 Adult Thoracic-Abdominal Ganglion    19.4 Adult Crop    96.4 Adult Midgut    14.4 Adult Hindgut    14.2 Adult Malpighian Tubules    4.1 Adult Fat Body    6.9 Adult Salivary Gland    10.6 Adult Heart    35.3 Adult VirginFemale Spermatheca    16.9 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    70.8 Adult Testis    12.7 Adult Male Accessory Gland    15 Adult Carcass    52.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    189.95 Larval Midgut    22.5 Larval Hindgut    15.2 Larval Malpighian Tubules    9 Larval Fat Body    11.3 Larval Salivary Gland    17.8 Larval Trachea    87.3 Larval Carcass    93.725 Adult Head    43.6 Adult Eye    82.95 Adult Brain    25.7 Adult Thoracic-Abdominal Ganglion    19.4 Adult Crop    96.4 Adult Midgut    14.4 Adult Hindgut    14.2 Adult Malpighian Tubules    4.1 Adult Fat Body    6.9 Adult Salivary Gland    10.6 Adult Heart    35.3 Adult VirginFemale Spermatheca    16.9 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    70.8 Adult Testis    12.7 Adult Male Accessory Gland    15 Adult Carcass    52.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    189.95 Larval Midgut    22.5 Larval Hindgut    15.2 Larval Malpighian Tubules    9 Larval Fat Body    11.3 Larval Salivary Gland    17.8 Larval Trachea    87.3 Larval Carcass    93.725 Adult Head    43.6 Adult Eye    82.95 Adult Brain    25.7 Adult Thoracic-Abdominal Ganglion    19.4 Adult Crop    96.4 Adult Midgut    14.4 Adult Hindgut    14.2 Adult Malpighian Tubules    4.1 Adult Fat Body    6.9 Adult Salivary Gland    10.6 Adult Heart    35.3 Adult VirginFemale Spermatheca    16.9 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    70.8 Adult Testis    12.7 Adult Male Accessory Gland    15 Adult Carcass    52.8 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    189.95 Larval Midgut    22.5 Larval Hindgut    15.2 Larval Malpighian Tubules    9 Larval Fat Body    11.3 Larval Salivary Gland    17.8 Larval Trachea    87.3 Larval Carcass    93.725 Adult Head    43.6 Adult Eye    82.95 Adult Brain    25.7 Adult Thoracic-Abdominal Ganglion    19.4 Adult Crop    96.4 Adult Midgut    14.4 Adult Hindgut    14.2 Adult Malpighian Tubules    4.1 Adult Fat Body    6.9 Adult Salivary Gland    10.6 Adult Heart    35.3 Adult VirginFemale Spermatheca    16.9 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    70.8 Adult Testis    12.7 Adult Male Accessory Gland    15 Adult Carcass    52.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    189.95 Larval Midgut    22.5 Larval Hindgut    15.2 Larval Malpighian Tubules    9 Larval Fat Body    11.3 Larval Salivary Gland    17.8 Larval Trachea    87.3 Larval Carcass    93.725 Adult Head    43.6 Adult Eye    82.95 Adult Brain    25.7 Adult Thoracic-Abdominal Ganglion    19.4 Adult Crop    96.4 Adult Midgut    14.4 Adult Hindgut    14.2 Adult Malpighian Tubules    4.1 Adult Fat Body    6.9 Adult Salivary Gland    10.6 Adult Heart    35.3 Adult VirginFemale Spermatheca    16.9 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    70.8 Adult Testis    12.7 Adult Male Accessory Gland    15 Adult Carcass    52.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    189.95 Larval Midgut    22.5 Larval Hindgut    15.2 Larval Malpighian Tubules    9 Larval Fat Body    11.3 Larval Salivary Gland    17.8 Larval Trachea    87.3 Larval Carcass    93.725 Adult Head    43.6 Adult Eye    82.95 Adult Brain    25.7 Adult Thoracic-Abdominal Ganglion    19.4 Adult Crop    96.4 Adult Midgut    14.4 Adult Hindgut    14.2 Adult Malpighian Tubules    4.1 Adult Fat Body    6.9 Adult Salivary Gland    10.6 Adult Heart    35.3 Adult VirginFemale Spermatheca    16.9 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    70.8 Adult Testis    12.7 Adult Male Accessory Gland    15 Adult Carcass    52.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    43.6   NA  Eye    82.95   NA  Brain    25.7   189.95  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    19.4   NA  Crop    96.4   22.5  Midgut    14.4   15.2  Hindgut    14.2   9  Malpighian Tubules    4.1   no informative data  Fat Body    6.9   17.8  Salivary Gland    no informative data   NA  Heart    35.3   87.3  Trachea    NA   NA  VirginFemale Spermatheca    16.9   NA  InseminatedFemale Spermatheca    22.5   NA  Ovary    70.8   NA  Testis    12.7   NA  Male Accessory Gland    no informative data   93.725  Carcass    52.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    24 central nervous system, pupae P8    25 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    8 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    24 central nervous system, pupae P8    25 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    8 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    24 central nervous system, pupae P8    25 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    8 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    24 central nervous system, pupae P8    25 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    8 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    24 central nervous system, pupae P8    25 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    8 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    24 central nervous system, pupae P8    25 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    8 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    24 central nervous system, pupae P8    25 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    8 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    24 central nervous system, pupae P8    25 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    9 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    4 carcass, larvae L3 wandering    7 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    10 ovary, mated 4-day female    8 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    8 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    6 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    10 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    4 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    2 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    8 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    6 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    10 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    4 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    2 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    8 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    6 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    10 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    4 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    2 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    8 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    6 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    10 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    4 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    2 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    8 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    6 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    10 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    4 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    2 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    8 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    6 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    10 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    4 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    2 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    8 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    6 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    10 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    4 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    2 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    12 embryo 10-12hr    6 embryo 12-14hr    8 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    6 larva L1    2 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    5 white prepupae 12hr    10 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    4 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    2 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    11 embryonic Kc167    8 embryonic S1    3 embryonic S3    23 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    17 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    17 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    11 embryonic Kc167    8 embryonic S1    3 embryonic S3    23 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    17 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    17 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    11 embryonic Kc167    8 embryonic S1    3 embryonic S3    23 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    17 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    17 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    11 embryonic Kc167    8 embryonic S1    3 embryonic S3    23 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    17 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    17 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    11 embryonic Kc167    8 embryonic S1    3 embryonic S3    23 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    17 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    17 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    11 embryonic Kc167    8 embryonic S1    3 embryonic S3    23 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    17 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    17 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    11 embryonic Kc167    8 embryonic S1    3 embryonic S3    23 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    17 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    17 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    11 embryonic Kc167    8 embryonic S1    3 embryonic S3    23 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    17 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    17 ovary fGS/OSS    10 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011666 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5099-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive msi1 viable msiEY07912 viable, with Scer\GAL4pnr-MD237 msiGD1490 Sterility Allele fertile msiEY07912 Other Phenotypes Allele meiotic cell cycle defective msi1 visible, with Scer\GAL4pnr-MD237 msiGD1490 visible | recessive msi1 wild-type msi+t10 msi+t14.3 Phenotype manifest in Allele external sensory organ msi1 germline stem cell | somatic clone msi1 glial cell & macrochaeta msi1 macrochaeta & scutum msi1 msi2 microchaeta & scutum msi1 msi2 neuron & macrochaeta msi1 neuron & microchaeta msi1 ommatidium msi1 rhabdomere msi1 socket msi1 spermatogonium | somatic clone msi1 testis msi1 msi2 msiunspecified testis (with msi1) msi2 testis (with msi2) msi1 testis sheath muscle msi1 msi2 testis sheath muscle (with msi1) msi2 testis sheath muscle (with msi2) msi1 thecogen cell msi1 thecogen cell & dorsal triple row msi1 thecogen cell & medial triple row msi1 thecogen cell & twin sensillum of margin 1 msi1 thecogen cell & twin sensillum of margin 2 msi1 tormogen cell, with Scer\GAL4pnr-MD237 msiGD1490 trichogen cell, with Scer\GAL4pnr-MD237 msiGD1490 trichogen cell | supernumerary msi1 trichogen cell | supernumerary, with Scer\GAL4pnr-MD237 msiGD1490
Classical Alleles ( 19 )
For All Classical Alleles Show
Allele of msi Class Mutagen Stocks Known lesion msi2 Delta2-3 3 -- msi1 amorphic allele - genetic evidence Delta2-3 2 Yes msiB455 1 -- msic01658 piggyBac transposase 1 -- msiC220 1 -- msid08770 P-element activity 1 -- msie04077 piggyBac transposase 1 -- msiEY07912 P-element activity 1 Yes msif00965 piggyBac transposase 1 -- msif01082 piggyBac transposase 1 -- msif03398 piggyBac transposase 1 -- msif04939 1 -- msif07665 piggyBac transposase 1 -- msiKG01341 1 -- msiMB03603 1 -- msiMI00977 1 -- msiMI01988 1 -- msiMI04354 1 -- msiunspecified 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of msi Class Mutagen Stocks Known lesion msiGD1490 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes msi+t10 in vitro construct - genomic fragment 0 Yes msi+t14.3 in vitro construct - genomic fragment 0 Yes msiA.B.Act5C in vitro construct - regulatory fusion 0 Yes msiA.B in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes msiAct5C.PO in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6203 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1490} NA characterization construct P{msi.A.B} NA P{msi+10} NA P{msi+14.3} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}ympEY07912 NA P{GSV1}GSd314GSd314 NA P{XP}msid08770 NA miscellaneous insertions Mi{MIC}msiMI00977 NA Mi{MIC}msiMI01988 NA Mi{MIC}msiMI04354 NA P{SUPor-P}msiKG01341 NA PBac{3HPy+}msiC220 NA PBac{5HPw+}msiB455 NA PBac{PB}msic01658 NA PBac{RB}msie04077 NA PBac{WH}msif00965 NA PBac{WH}msif01082 NA PBac{WH}msif03398 NA PBac{WH}msif04939 NA PBac{WH}msif07665 NA insertion of enhancer trap binary system Mi{ET1}msiMB03603 No
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cellular response to hypoxia inferred from mutant phenotype (Lee et al., 2008) chaeta development inferred from mutant phenotype (Nakamura et al., 1994) germ-line stem cell division inferred from mutant phenotype (Siddall et al., 2006) negative regulation of translation inferred from mutant phenotype (Okabe et al., 2001) stem cell maintenance inferred from mutant phenotype (Siddall et al., 2006)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Nakamura et al., 1994)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
mRNA binding inferred from sequence or structural similarity (Lasko, 2000) inferred from sequence or structural similarity with Saccharomyces HRP1 (FlyBase, 1992-) non-traceable author statement (Montell, 1994.5.19) traceable author statement (Bardin et al., 2004) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-) RNA binding inferred from sequence model (Nakamura et al., 1994) translation regulator activity traceable author statement (Dean et al., 2002) translation repressor activity, nucleic acid binding traceable author statement (Bardin et al., 2004)
Biological Process
CV term Evidence References
cell fate determination traceable author statement (Bardin et al., 2004) mRNA processing inferred from sequence or structural similarity with Saccharomyces HRP1 (FlyBase, 1992-) negative regulation of translation traceable author statement (Bardin et al., 2004) regulation of translation traceable author statement (Dean et al., 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
msi - Df31 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) msi - Hsp68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) msi - ps experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions msi allele Gene References msi1 sina (Hirota et al., 1999, Okabe et al., 2001) ttk (Hirota et al., 1999, Okabe et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68000 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011666 DroID - A comprehensive database of gene and protein interactions. • FBgn0011666 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43087
Orthologs
OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search No orthologs identified Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (None identified)
No orthologies identified
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\msi Drosophila sechellia Dsec\GM10251 Drosophila erecta Dere\GG11413 Drosophila yakuba Dyak\GE23609 Drosophila ananassae Dana\GF17697 Drosophila pseudoobscura pseudoobscura Dpse\GA27393 Drosophila willistoni Dwil\GK12850 Drosophila virilis Dvir\GJ10967 Drosophila mojavensis Dmoj\GI22407 Drosophila grimshawi Dgri\GH18982
Stocks & Reagents
Stocks Listed in FlyBase ( 23 )
Bloomington	4160 msi1/TM3, Sb1 4161 msi2/TM3, Sb1
Harvard	- P{XP}msid08770 - PBac{PB}msic01658 - PBac{RB}msie04077 - PBac{WH}msif00965 - PBac{WH}msif01082 - PBac{WH}msif03398 - PBac{WH}msif04939 - PBac{WH}msif07665
Kyoto	107603 msi1/TM3, Sb1
VDRC	v11784 w1118 P{GD1490}v11784
	More stocks available...
Genomic Clones ( 3 )
	BACR03M22 BACR03O03 BACR09D08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 178 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD15419 LD31631 RH49436
Other clones	DPiM_LD31631 FI20028 MIP15164
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH27520 HL03681 LD02360 RE11238 RE16978 RE64201 RH29067 RH60565
Other clones	10230 11879 12698 12814 12836 13188 16605 28460 28534 29317 31912 35809 37575 45102 47770 51171 54762 55522 56828 58583 61785 72660 76450 77408 78510 100938 102062 102218 102237 106392 106871 108272 109167 119458 125463 146649 147281 147415 147449 147748 155635 158146 162319 162634 176541 180910 190425 191617 214249.58 274739 281498 290847 295479 295790 527067 AI124317 CK01.9D.H8 CK01.20D.C9 CK01.22B.B10 CK01.28A.B6 CK01.30D.G6 CK01.34C.G6 CK01.60C.D2 CK01.66D.D10 CK01.87C.H4 CK01.89D.E1 CK01.104C.D5 CK01.106C.H3 CK01.120B.E1 CK01.120B.E2 Dmel_neb_010337_0150_2828 Dmel_neb_011629_0608_3592 Dmel_neb_014484_0356_1547 EC090971 EC257260 EK016304 EK019111 EK029359 EK053947 EK062805 EK069162 EK070617 EK081130 EK087615 EK089030 EK091817 EK091849 EK102507 EK106409 EK109620 EK112749 EK113458 EK114134 EK114246 EK114914 EK129106 EK136342 EK136363 EK137328 EK140202 EK142534 EK155929 EK158846 EK160648 EK162131 EK166234 EK170423 EK179758 EK181236 EK182334 EK187464 EK206530 EK207201 EK207521 EK210757 EK223424 EK224264 EK225609 EK234311 EK237802 EK239131 EK242732 EK247527 EK249261 EK249954 EK263911 EK265722 EK281311 EK282708 EK286049 EK292640 EK294318 EK302335 EK306721 EN01204 EN05061 EN07222 EN09024 EN12908 EP10034 EP11964 RP003066838 RP003072358 RP003123286 RP003125700 RP003156714 RP003226770 RP003228176 RP003243779 RP003287515 RP003310506 RP003324317 RP003349310 RP003391370 RP003406438 RP003424550 RP003511300 RP003529285 RP003609346 RP003633795 RP003651127 RP003672426 RP003676261 RP003680155
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011666 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43087
Antibody Information
	polyclonal (Nakamura et al., 1994)
Other Information
Discoverer
Etymology
	The mutant is referred to as musashi, owing to the frequent double-shaft phenotype. Typical samuri warriors used only one sword, Musashi Miyamoto originated the style of fighting using two swords simultaneously. (Nakamura et al., 1994)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	mir-1000 is located within an intron of msi. (Stark et al., 2007)
Other Comments
	msi is required in spermatocytes for correct meiotic chromosome segregation and cytokinesis. (Siddall et al., 2006) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) msi protein acts as a translational repressor of ttk in the neuronal lineage, acting in the 3' untranslated region of the ttk mRNA. N signalling does not affect msi expression, though msi can act downstream of N, therefore N signalling is more likely to regulate msi activity than its expression. (Okabe et al., 2001) msi is epistatic to N during the asymmetric cell division giving rise to external sensory organ precursor IIa and IIb cells. (Okabe et al., 2001) msi functions to regulate ttk expression. (Hirota et al., 1999) msi and sina function redundantly in R1 and R6 to down-regulate ttk in the developing photoreceptor cells. (Hirota et al., 1999) msi is required for the development of adult external sensory organs (sensilla). msi mutation typically results in extra sockets and/or shafts. It is suggested that the msi phenotype results from transformation of a neuron/glial-precursor cell into a second shaft/socket-precursor cell. It is proposed that the msi protein regulates sensillum development by controlling the expression of target genes at the post-transcriptional level. (Nakamura et al., 1994) msi is required for development of adult external sensory organs (sensilla). Mutations in msi result in the appearance of extra outer support cells. It is proposed that the msi protein regulates sensillum development by controlling the expression of target genes at the posttranscriptional level. (Nakamura et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079838 NM_170229 NM_001260374 NM_001260375 NM_001276030 NM_001276031 NM_001276032 RefSeq (Proteins) • NP_524577 NP_733108 NP_001247303 NP_001247304 NP_001262959 NP_001262960 NP_001262961 DDBJ / EMBL / GenBank • AA440590 AA951254 AAF56478 AAK93226 AAM51031 AAN14056 AAO25031 AF067892 AFH06620 AFH06621 AGB96339 AGB96340 AGB96341 AI124317 AW943094 AY051802 AY119171 BG638091 BI618573 BT003274 CAA55897 CAA55898 CL610192 CZ467336 CZ476403 CZ481832 CZ483441 CZ483442 CZ483571 CZ483572 CZ485958 CZ490401 X79339 X79340 X79408 Entrez Gene - A searchable database of RefSeq genes. • 43087 UniProtKB/TrEMBL • Q7JN15 Q8IMS8 Q8MS04 Q9VBQ1 Q86PA1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68000 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011666 DroID - A comprehensive database of gene and protein interactions. • FBgn0011666 DRSC - Results from RNAi screens. • FBgn0011666 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011666 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5099-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011666 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43087 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /neural/musashi1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43087 modMine - Data generated by the modENCODE project. • FBgn0011666
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	anon-EST:Liang-2.35   CG5099 (Lee et al., 2008) clone 2.35 (Liang and Biggin, 1998) DMSIDNA (Colson and Goldstein, 1999) msi (Sofola et al., 2007, Blanco et al., 2010, Fabre et al., 2008, Geng and MacDonald, 2007, Minidorff et al., 2007, Kankel et al., 2007, Hilgers et al., 2011, Lee et al., 2008, Mensch et al., 2008, Hilgers et al., 2012, Siddall et al., 2009, Stark et al., 2007, Hazelett et al., 2009, Smibert et al., 2012, Mackay et al., 2012) Msi (Siddall et al., 2006, Hime et al., 2007) MSI (Sofola et al., 2007) musashi (Smibert et al., 2012) SIDNA (Colson and Goldstein, 1999)
Name Synonym	musashi (Colson, 1998.5.26, Mummery-Widmer et al., 2009) mushashi (Stark et al., 2007)
Secondary FlyBase IDs
References ( 65 )
Generate a list of	All references relating to this gene ( 65 )
List References by type	Research paper  ( 24 ) Supplementary material  ( 8 ) Review  ( 7 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 5 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Hilgers et al., 2012, Genes Dev. 26(20): 2259--2264 ELAV mediates 3' UTR extension in the Drosophila nervous system. [FBrf0219699] Mackay et al., 2012, Nature 482(7384): 173--178 The Drosophila melanogaster Genetic Reference Panel. [FBrf0217434] Smibert et al., 2012, Cell Rep. 1(3): 277--289 Global Patterns of Tissue-Specific Alternative Polyadenylation in Drosophila. [FBrf0218523] Hilgers et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(38): 15864--15869 Neural-specific elongation of 3' UTRs during Drosophila development. [FBrf0215804] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...