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Gene Dmel\insc

General Information
Symbol	Dmel\insc	Species	D. melanogaster
Name	inscuteable	Annotation symbol	CG11312
Feature type	protein_coding_gene	FlyBase ID	FBgn0011674
Gene Model Status	Current	Stock availability	29 publicly available
Also Known As	nem, fam
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	57B3-57B3	Sequence location	2R:16,708,767..16,723,533 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene inscuteable is referred to in FlyBase by the symbol Dmel\insc (CG11312, FBgn0011674). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: cytoskeletal adaptor activity; protein binding. There is experimental evidence for 11 unique biological process terms, many of which group under: localization; system development; biological regulation; single-organism developmental process; cell fate commitment; multicellular organismal development; cellular component organization or biogenesis; neuroblast fate commitment; apical protein localization; renal tubule development; somatic muscle development; regulation of developmental process; RNA localization; regulation of asymmetric cell division; cell cycle; stem cell division. 77 alleles are reported. The phenotypes of these alleles are annotated with: hypodermal muscle of larval abdomen; ISN innervated muscle of A1-7; organ system; A1-7 transverse muscle; organ system subdivision; hypodermal muscle of metathorax; thoracic segment; abdominal adult muscle precursor cell; hypodermal muscle of larval abdominal 2; embryonic/larval neuron. It has one annotated transcript and one annotated polypeptide. Protein features are: Armadillo-like helical; Armadillo-type fold. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 06-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, adult midgut, adult heart, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1625035_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of insc. Gene sequence location is 2R:16708771..16723569.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms defense response to Gram-negative bacterium
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 57B3-57B3   Limits computationally determined from genome sequence between P{lacW}l(2)k06409k06409&P{PZ}insc05475 and P{PZ}l(2)0780607806
Experimentally Determined Cytological Location
Cytogenetic map Notes References 57B1-57B4   (determined by in situ hybridisation)   (Abdelilah-Seyfried et al., 2000) 57B1-57B4     (Kraut, 1994.12.1) 57B1-57B20     (Knirr, 1996.10.6) 57B1-57B5   (determined by in situ hybridisation)   (Kania et al., 1995) 57B-57B   (determined by in situ hybridisation)   (Burchard et al., 1995) 57B-57B   57B1--3 57B1--5 57B2--3 57B3--5   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Knirr, 1996.10.6, Kraut, 1994.12.1)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\insc for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.51
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) insc-RA FBtr0071562  NM_057676   3887   859
Additional Transcript Data & Comments
Reported size (kB)	4.3 (northern blot) (Knirr et al., 1997) 4.2 (northern blot) (Kraut and Campos-Ortega, 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein insc-PA   FBpp0071490   95.0   859   6.68     NP_477024   AAF46626
Additional Polypeptide Data & Comments
Reported size (kDa)	859 (aa) (Kraut and Campos-Ortega, 1996) 767 (aa); 84 (kD predicted) (Knirr et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Armadillo-type fold (IPR016024) Armadillo-like helical (IPR011989)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141052   AA141053   AE013599 AAF46626   AC004433   AQ074114   BG639681   BI232705   BI485650   BT021251 AAX33399   CL632195   CO268473   CO288494   CO312127   CO344048   CO345158   CZ470932   CZ471852   CZ475270   CZ475271   EC080520   EC207620   EC217242   EC242687   EC248115   EC253992   EC256253   G00585   GH740207   GH741759   GH742895   GH743026   GH746842   GH747255   GH764184   GH764690   GH765352   GH766157   GH766865   GH769685   U73489 AAB18232   X83111 CAA58175
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9W2R4 Q5BIH3 Q24367 Q94543
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 6   central brain anlage in statu nascendi   (Fisher et al., 2012) head mesoderm anlage   (Fisher et al., 2012) antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage head mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium head mesoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 9 -- 11   neuroblast of ventral nerve cord primordium   (Cai et al., 2001) embryonic stage 9 -- 17   procephalic neuroblast   (Cai et al., 2001) thoracic neuroblast   (Cai et al., 2001) abdominal neuroblast   (Cai et al., 2001) embryonic stage 10 -- 12   C1 chordotonal organ precursor cell   (Knirr et al., 1997) posterior midgut primordium   (Kraut and Campos-Ortega, 1996) anterior midgut primordium   (Kraut and Campos-Ortega, 1996) embryonic stage 11 -- 12   adult anterior midgut primordium   (Fisher et al., 2012) adult posterior midgut primordium   (Fisher et al., 2012) antennal primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium anterior midgut primordium   (Fisher et al., 2012) dorsal sensory complex primordium   (Fisher et al., 2012) germline cell   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) procephalic neuroblast   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) ventral sensory complex anlage   (Fisher et al., 2012) embryonic stage 13 -- 16   adult midgut precursor cell   (Fisher et al., 2012) amnioserosa   (Fisher et al., 2012) apoptotic amnioserosa   (Fisher et al., 2012) dorsal sensory complex proper primordium   (Fisher et al., 2012) Comment:reported as dorsal/lateral sensory complexes embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic neuroblast of embryonic central brain   (Fisher et al., 2012) embryonic small intestine   (Fisher et al., 2012) neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) ventral sensory complex primordium   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral nerve cord | restricted   (Kraut and Campos-Ortega, 1996) embryonic stage 1 -- 14   embryonic brain   (Knirr et al., 1997) presumptive embryonic/larval central nervous system   (Knirr et al., 1997) embryonic stage 4   procephalic neurogenic region   (Knirr et al., 1997) embryonic stage 6   procephalic neurogenic region   (Kraut and Campos-Ortega, 1996) embryonic stage 7 -- 15     (Kraut and Campos-Ortega, 1996) Comment:reference states 3-12 hr AEL embryonic stage 9 -- 11   neuroblast   (Knirr et al., 1997, Kraut and Campos-Ortega, 1996) ventral neurectoderm   (Knirr et al., 1997) embryonic stage 9 -- 17     (Knirr et al., 1997) Comment:reference states 4-20 hr AEL embryonic stage 10   ganglion mother cell   (Kraut and Campos-Ortega, 1996) embryonic stage 10 -- 12   presumptive embryonic/larval peripheral nervous system | restricted   (Knirr et al., 1997) embryonic stage 10 -- 13   myoblast of embryonic/larval muscle system   (Kraut and Campos-Ortega, 1996) embryonic stage 11 -- 13   sense organ | precursor   (Kraut and Campos-Ortega, 1996) embryonic stage 13 -- 15   embryonic/larval midgut   (Kraut and Campos-Ortega, 1996) embryonic stage 15 -- 17   Malpighian tubule   (Kraut and Campos-Ortega, 1996) gastric caecum   (Kraut and Campos-Ortega, 1996) embryonic/larval hindgut   (Kraut and Campos-Ortega, 1996) embryonic/larval pharynx   (Kraut and Campos-Ortega, 1996) embryonic/larval trachea   (Kraut and Campos-Ortega, 1996) adult stage     (Knirr et al., 1997)
Additional Descriptive Data
	insc transcript is detected in 4-20 hr embryos, and in adult male and female flies. insc transcript is first detected during the cellular blastoderm stage within the procephalic neurogenic region. The region of expression extends dorsally at stage 6/7. Expression is subsequently detected in individual cells within the embryonic brain until late embryogenesis. During germ band extension, insc transcript expression is detected in the neurectoderm, and in early germ band extended embryos, the full wave of delaminating neuroblasts express insc transcript. In the developing CNS, most, if not all, neuroblasts express insc. Transient expression in some putative PNS precursors (external sense organ precursors and Ich5 precursor) is detected in 5 hr embryos. No CNS expression is detected after stage 14 of embryogenesis. (Li et al., 1997, Knirr et al., 1997) insc transcripts are detected in 3-12hr embryos on northern blots. Transcripts are first detected by in situ hybridization at stage 6 in the procephalic neurectoderm. Immediately thereafter, they are detected in neuroblasts of the first segregation wave after they have begun to delaminate. At later embryonic stages, the transcript expression pattern is stated to be the same as the distribution of insc protein which is as follows. In stage 10, expression is detected in two cells per segment in the lateral epidermis that are thought to be the first two cells that will give rise to the larval peripheral sense organs. Expression is lost in developing sense organs by the time of dorsal closure but remains in the CNS until after it has condensed. In stages 10-13, expression is seen in mesodermal cells presumed to be muscle precursors and in the posterior midgut primordia. Strong expression is seen at stage 15 in the pharynx, gastric caecae, hindgut, tracheal branches, and Malpighian tubules. (Kraut and Campos-Ortega, 1996, Knirr et al., 1997)
Marker for
Subcellular Localization
CV Term	plasma membrane | apical & proximal to cytoplasm (Li et al., 1997)
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral nerve cord | restricted   (Kraut and Campos-Ortega, 1996) embryonic stage 6   procephalic neurogenic region   (Kraut and Campos-Ortega, 1996) embryonic stage 9 -- 11   neuroblast   (Kraut and Campos-Ortega, 1996) embryonic stage 10   ganglion mother cell   (Kraut and Campos-Ortega, 1996) neuroblast | apical   • cytoplasm | apical (Bulgheresi et al., 2001) Comment:metaphase neuroblast   • cytoplasm (Bulgheresi et al., 2001) Comment:telophase embryonic stage 10 -- 11   neuroblast of ventral nerve cord primordium   (Cai et al., 2001) embryonic stage 10 -- 12   anterior midgut primordium   (Kraut and Campos-Ortega, 1996) posterior midgut primordium   (Kraut and Campos-Ortega, 1996) embryonic stage 10 -- 13   myoblast of embryonic/larval muscle system   (Kraut and Campos-Ortega, 1996) embryonic stage 10 -- 17   procephalic neuroblast   (Cai et al., 2001) thoracic neuroblast   (Cai et al., 2001) abdominal neuroblast   (Cai et al., 2001) embryonic stage 11   ganglion mother cell GMC1-1a | apical   (Bhat and Apsel, 2004) embryonic stage 11 -- 13   sense organ | precursor   (Kraut and Campos-Ortega, 1996) embryonic stage 13 -- 15   embryonic/larval midgut   (Kraut and Campos-Ortega, 1996) embryonic stage 15 -- 17   embryonic/larval trachea   (Kraut and Campos-Ortega, 1996) Malpighian tubule   (Kraut and Campos-Ortega, 1996) gastric caecum   (Kraut and Campos-Ortega, 1996) embryonic/larval hindgut   (Kraut and Campos-Ortega, 1996) embryonic/larval pharynx   (Kraut and Campos-Ortega, 1996) pupal stage P6 -- pharate adult stage P15   wing disc | restricted   (Kraut and Campos-Ortega, 1996) Comment:reference states >20 hr APF
Additional Descriptive Data
	insc protein localizes to the apical end of GMC1-1. (Kraut and Campos-Ortega, 1996, Bhat and Apsel, 2004) insc protein is first detected at stage 6 in the procephalic neurectoderm and later in the delaminating neuroblasts of the ventral nerve cord. At late stage 10, insc protein is detected in two cells per segment in the lateral epidermis that are thought to be the first two cells that will give rise to the larval peripheral sense organs. Expression is lost in developing sense organs by the time of dorsal closure but remains in the CNS until after it has condensed. In stages 10-13, expression is seen in mesodermal cells presumed to be muscle precursors and in the posterior midgut primordia. Strong expression is seen at stage 15 in the pharynx, gastric caecae, hindgut, tracheal branches, and Malpighian tubules. insc protein is also detected in pupal wing discs older than about 20hr after puparium formation. It is present in the intervein spaces and is excluded from the veins themselves. (Yu et al., 2000, Kraut and Campos-Ortega, 1996) The cell cycle dependence of insc protein localization was studied in neuroblasts. Protein is first detected during neuroblast delamination in a stalk that maintains contact with the epithelial surface. In delaminated neuroblasts, insc protein forms a crescent along the apical cell cortex. Shortly after delamination, neuroblasts enter mitosis. The apical crescent of insc protein persists through prophase and metaphase and becomes delocalized in anaphase. insc protein does not preferentially segregate into one of the daughter cells. insc protein localization is dependent on actin filaments but is not affected by destruction of microtubules. (Kraut et al., 1996)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
apical cortex inferred from direct assay (Kraut et al., 1996, Li et al., 1997, Siegrist and Doe, 2005, Nipper et al., 2007, Fuerstenberg et al., 1998) cell cortex inferred from direct assay (Akiyama-Oda et al., 2000) cytoplasm inferred from direct assay (Li et al., 1997)
Expression Deduced from Reporters
Reporter: P{lacZ}inscAB44 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral nerve cord | restricted   (Kraut and Campos-Ortega, 1996) embryonic stage 6   procephalic neurogenic region   (Kraut and Campos-Ortega, 1996) embryonic stage 9 -- 11   neuroblast   (Kraut and Campos-Ortega, 1996) embryonic stage 10   ganglion mother cell   (Kraut and Campos-Ortega, 1996) embryonic stage 10 -- 12   anterior midgut primordium   (Kraut and Campos-Ortega, 1996) posterior midgut primordium   (Kraut and Campos-Ortega, 1996) embryonic stage 10 -- 13   myoblast of embryonic/larval muscle system   (Kraut and Campos-Ortega, 1996) embryonic stage 11 -- 13   sense organ | precursor   (Kraut and Campos-Ortega, 1996) embryonic stage 13 -- 15   embryonic/larval midgut   (Kraut and Campos-Ortega, 1996) embryonic stage 15 -- 17   gastric caecum   (Kraut and Campos-Ortega, 1996) Malpighian tubule   (Kraut and Campos-Ortega, 1996) embryonic/larval hindgut   (Kraut and Campos-Ortega, 1996) embryonic/larval trachea   (Kraut and Campos-Ortega, 1996) embryonic/larval pharynx   (Kraut and Campos-Ortega, 1996) adult stage & oogenesis   follicle cell   (Kraut and Campos-Ortega, 1996)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, adult midgut, adult heart, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    278.85 Larval Midgut    55.3 Larval Hindgut    63.1 Larval Malpighian Tubules    15 Larval Fat Body    57.6 Larval Salivary Gland    19.1 Larval Trachea    26.475 Larval Carcass    39.2 Adult Head    31.1 Adult Eye    13.1 Adult Brain    6 Adult Thoracic-Abdominal Ganglion    10.2 Adult Crop    127 Adult Midgut    127.9 Adult Hindgut    75.4 Adult Malpighian Tubules    12.3 Adult Fat Body    66 Adult Salivary Gland    23.5 Adult Heart    122.475 Adult VirginFemale Spermatheca    35.3 Adult InseminatedFemale Spermatheca    51.7 Adult Ovary    108.5 Adult Testis    12 Adult Male Accessory Gland    9.7 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    278.85 Larval Midgut    55.3 Larval Hindgut    63.1 Larval Malpighian Tubules    15 Larval Fat Body    57.6 Larval Salivary Gland    19.1 Larval Trachea    26.475 Larval Carcass    39.2 Adult Head    31.1 Adult Eye    13.1 Adult Brain    6 Adult Thoracic-Abdominal Ganglion    10.2 Adult Crop    127 Adult Midgut    127.9 Adult Hindgut    75.4 Adult Malpighian Tubules    12.3 Adult Fat Body    66 Adult Salivary Gland    23.5 Adult Heart    122.475 Adult VirginFemale Spermatheca    35.3 Adult InseminatedFemale Spermatheca    51.7 Adult Ovary    108.5 Adult Testis    12 Adult Male Accessory Gland    9.7 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    278.85 Larval Midgut    55.3 Larval Hindgut    63.1 Larval Malpighian Tubules    15 Larval Fat Body    57.6 Larval Salivary Gland    19.1 Larval Trachea    26.475 Larval Carcass    39.2 Adult Head    31.1 Adult Eye    13.1 Adult Brain    6 Adult Thoracic-Abdominal Ganglion    10.2 Adult Crop    127 Adult Midgut    127.9 Adult Hindgut    75.4 Adult Malpighian Tubules    12.3 Adult Fat Body    66 Adult Salivary Gland    23.5 Adult Heart    122.475 Adult VirginFemale Spermatheca    35.3 Adult InseminatedFemale Spermatheca    51.7 Adult Ovary    108.5 Adult Testis    12 Adult Male Accessory Gland    9.7 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    278.85 Larval Midgut    55.3 Larval Hindgut    63.1 Larval Malpighian Tubules    15 Larval Fat Body    57.6 Larval Salivary Gland    19.1 Larval Trachea    26.475 Larval Carcass    39.2 Adult Head    31.1 Adult Eye    13.1 Adult Brain    6 Adult Thoracic-Abdominal Ganglion    10.2 Adult Crop    127 Adult Midgut    127.9 Adult Hindgut    75.4 Adult Malpighian Tubules    12.3 Adult Fat Body    66 Adult Salivary Gland    23.5 Adult Heart    122.475 Adult VirginFemale Spermatheca    35.3 Adult InseminatedFemale Spermatheca    51.7 Adult Ovary    108.5 Adult Testis    12 Adult Male Accessory Gland    9.7 Adult Carcass    47.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    278.85 Larval Midgut    55.3 Larval Hindgut    63.1 Larval Malpighian Tubules    15 Larval Fat Body    57.6 Larval Salivary Gland    19.1 Larval Trachea    26.475 Larval Carcass    39.2 Adult Head    31.1 Adult Eye    13.1 Adult Brain    6 Adult Thoracic-Abdominal Ganglion    10.2 Adult Crop    127 Adult Midgut    127.9 Adult Hindgut    75.4 Adult Malpighian Tubules    12.3 Adult Fat Body    66 Adult Salivary Gland    23.5 Adult Heart    122.475 Adult VirginFemale Spermatheca    35.3 Adult InseminatedFemale Spermatheca    51.7 Adult Ovary    108.5 Adult Testis    12 Adult Male Accessory Gland    9.7 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    278.85 Larval Midgut    55.3 Larval Hindgut    63.1 Larval Malpighian Tubules    15 Larval Fat Body    57.6 Larval Salivary Gland    19.1 Larval Trachea    26.475 Larval Carcass    39.2 Adult Head    31.1 Adult Eye    13.1 Adult Brain    6 Adult Thoracic-Abdominal Ganglion    10.2 Adult Crop    127 Adult Midgut    127.9 Adult Hindgut    75.4 Adult Malpighian Tubules    12.3 Adult Fat Body    66 Adult Salivary Gland    23.5 Adult Heart    122.475 Adult VirginFemale Spermatheca    35.3 Adult InseminatedFemale Spermatheca    51.7 Adult Ovary    108.5 Adult Testis    12 Adult Male Accessory Gland    9.7 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    278.85 Larval Midgut    55.3 Larval Hindgut    63.1 Larval Malpighian Tubules    15 Larval Fat Body    57.6 Larval Salivary Gland    19.1 Larval Trachea    26.475 Larval Carcass    39.2 Adult Head    31.1 Adult Eye    13.1 Adult Brain    6 Adult Thoracic-Abdominal Ganglion    10.2 Adult Crop    127 Adult Midgut    127.9 Adult Hindgut    75.4 Adult Malpighian Tubules    12.3 Adult Fat Body    66 Adult Salivary Gland    23.5 Adult Heart    122.475 Adult VirginFemale Spermatheca    35.3 Adult InseminatedFemale Spermatheca    51.7 Adult Ovary    108.5 Adult Testis    12 Adult Male Accessory Gland    9.7 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    278.85 Larval Midgut    55.3 Larval Hindgut    63.1 Larval Malpighian Tubules    15 Larval Fat Body    57.6 Larval Salivary Gland    19.1 Larval Trachea    26.475 Larval Carcass    39.2 Adult Head    31.1 Adult Eye    13.1 Adult Brain    6 Adult Thoracic-Abdominal Ganglion    10.2 Adult Crop    127 Adult Midgut    127.9 Adult Hindgut    75.4 Adult Malpighian Tubules    12.3 Adult Fat Body    66 Adult Salivary Gland    23.5 Adult Heart    122.475 Adult VirginFemale Spermatheca    35.3 Adult InseminatedFemale Spermatheca    51.7 Adult Ovary    108.5 Adult Testis    12 Adult Male Accessory Gland    9.7 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    31.1   NA  Eye    13.1   NA  Brain    6   278.85  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    10.2   NA  Crop    127   55.3  Midgut    127.9   63.1  Hindgut    75.4   15  Malpighian Tubules    no informative data   57.6  Fat Body    66   19.1  Salivary Gland    no informative data   NA  Heart    122.475   26.475  Trachea    NA   NA  VirginFemale Spermatheca    35.3   NA  InseminatedFemale Spermatheca    51.7   NA  Ovary    108.5   NA  Testis    12   NA  Male Accessory Gland    9.7   39.2  Carcass    47.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    11 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    3 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    4 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    11 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    3 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    4 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    11 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    3 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    4 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    11 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    3 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    4 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    11 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    3 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    4 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    11 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    3 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    4 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    11 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    3 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    4 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    11 central nervous system, pupae P8    1 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    3 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    9 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    4 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 06-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    5 embryo 04-06hr    14 embryo 06-08hr    35 embryo 08-10hr    33 embryo 10-12hr    27 embryo 12-14hr    16 embryo 14-16hr    18 embryo 16-18hr    21 embryo 18-20hr    12 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    6 white prepupae new    11 white prepupae 12hr    7 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    7 adult female 05day    6 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    5 embryo 04-06hr    14 embryo 06-08hr  (35) embryo 08-10hr  (33) embryo 10-12hr  (27) embryo 12-14hr    16 embryo 14-16hr    18 embryo 16-18hr    21 embryo 18-20hr    12 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    6 white prepupae new    11 white prepupae 12hr    7 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    7 adult female 05day    6 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    5 embryo 04-06hr    14 embryo 06-08hr    35 embryo 08-10hr    33 embryo 10-12hr    27 embryo 12-14hr    16 embryo 14-16hr    18 embryo 16-18hr    21 embryo 18-20hr    12 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    6 white prepupae new    11 white prepupae 12hr    7 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    7 adult female 05day    6 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    5 embryo 04-06hr    14 embryo 06-08hr    35 embryo 08-10hr    33 embryo 10-12hr    27 embryo 12-14hr    16 embryo 14-16hr    18 embryo 16-18hr    21 embryo 18-20hr    12 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    6 white prepupae new    11 white prepupae 12hr    7 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    7 adult female 05day    6 adult female 30day    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    5 embryo 04-06hr    14 embryo 06-08hr    35 embryo 08-10hr    33 embryo 10-12hr    27 embryo 12-14hr    16 embryo 14-16hr    18 embryo 16-18hr    21 embryo 18-20hr    12 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    6 white prepupae new    11 white prepupae 12hr    7 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    7 adult female 05day    6 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    5 embryo 04-06hr    14 embryo 06-08hr  (35) embryo 08-10hr  (33) embryo 10-12hr  27 embryo 12-14hr    16 embryo 14-16hr    18 embryo 16-18hr    21 embryo 18-20hr    12 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    6 white prepupae new    11 white prepupae 12hr    7 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    7 adult female 05day    6 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    5 embryo 04-06hr    14 embryo 06-08hr    35 embryo 08-10hr    33 embryo 10-12hr    27 embryo 12-14hr    16 embryo 14-16hr    18 embryo 16-18hr    21 embryo 18-20hr    12 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    6 white prepupae new    11 white prepupae 12hr    7 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    7 adult female 05day    6 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    5 embryo 04-06hr    14 embryo 06-08hr    35 embryo 08-10hr    33 embryo 10-12hr    27 embryo 12-14hr    16 embryo 14-16hr    18 embryo 16-18hr    21 embryo 18-20hr    12 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    6 white prepupae new    11 white prepupae 12hr    7 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    7 adult female 05day    6 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    1 embryonic GM2    11 embryonic Kc167    13 embryonic S1    3 embryonic S3    7 leg disc CME L1    2 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    8 ovary OSC    5 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    1 embryonic GM2    11 embryonic Kc167    13 embryonic S1    3 embryonic S3    7 leg disc CME L1    2 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    8 ovary OSC    5 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    1 embryonic GM2    11 embryonic Kc167    13 embryonic S1    3 embryonic S3    7 leg disc CME L1    2 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    8 ovary OSC    5 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    1 embryonic GM2    11 embryonic Kc167    13 embryonic S1    3 embryonic S3    7 leg disc CME L1    2 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    8 ovary OSC    5 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    1 embryonic GM2    11 embryonic Kc167    13 embryonic S1    3 embryonic S3    7 leg disc CME L1    2 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    8 ovary OSC    5 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    1 embryonic GM2    11 embryonic Kc167    13 embryonic S1    3 embryonic S3    7 leg disc CME L1    2 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    8 ovary OSC    5 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    1 embryonic GM2    11 embryonic Kc167    13 embryonic S1    3 embryonic S3    7 leg disc CME L1    2 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    8 ovary OSC    5 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    1 embryonic GM2    11 embryonic Kc167    13 embryonic S1    3 embryonic S3    7 leg disc CME L1    2 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    8 ovary OSC    5 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_31 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • PC00157 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011674 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11312-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for insc Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | adult stage | recessive insck02608 lethal | embryonic stage | recessive insck07505 insck07701 insck12405 inscP8 lethal | recessive insc03679 inscAB44 insck02517 insck10907 insck13706 inscΔ13 partially lethal - majority die insc05475 viable inscEY09668 viable, with Scer\GAL4arm.PS inscScer\UAS.cen.T:Avic\GFP inscScer\UAS.T:Avic\GFP viable, with Scer\GAL4eg-Mz360 inscScer\UAS.cen.T:Avic\GFP inscScer\UAS.T:Avic\GFP viable, with Scer\GAL4en-e16E inscScer\UAS.cen.T:Avic\GFP inscScer\UAS.T:Avic\GFP viable, with Scer\GAL4pnr-MD237 inscGD7267 Sterility Allele fertile inscEY09668 Other Phenotypes Allele cell polarity defective, with Scer\GAL4neur-P72 inscScer\UAS.cKa cell polarity defective, with Scer\GAL4sca-P309 inscScer\UAS.cKa neuroanatomy defective insc22 neuroanatomy defective | embryonic stage insc22 neuroanatomy defective | somatic clone insc22 visible, with Scer\GAL4sca-537.4 inscEP2010 wild-type insc3'DraIII.hs.T:Hsap\MYC insc3'RI.hs.T:Hsap\MYC insc5'NheI.hs.T:Zzzz\FLAG inscA2-A3.hs.T:Zzzz\FLAG inschs.PK Phenotype manifest in Allele abdominal 1 dorsal acute muscle 1 inscP49 abdominal 1 dorsal acute muscle 3 inscP49 abdominal 1 dorsal oblique muscle 1 inscP49 abdominal 1 dorsal oblique muscle 4 inscP8 abdominal 1 dorsal oblique muscle 5 inscP49 abdominal 1 dorsal transverse muscle 1 inscP8 abdominal 1 lateral adult muscle precursor cell insc8 abdominal 1 lateral longitudinal muscle 1 insc3 abdominal 1 lateral pentascolopidial chordotonal organ lch5 insc3 abdominal 1 lateral transverse muscle 1 inscP8 abdominal 1 lateral transverse muscle 2 inscP8 abdominal 1 lateral transverse muscle 3 insc8 inscP8 abdominal 1 lch5 neuron | embryonic stage inscΔ13 abdominal 1 segment border muscle insc3 abdominal 1 ventral longitudinal muscle insc3 abdominal 1 ventral oblique muscle insc8 abdominal 1 ventral oblique muscle 1 insc3 abdominal 1 ventral oblique muscle 2 insc3 abdominal 1 ventral oblique muscle 3 insc3 abdominal 2 dorsal acute muscle 1 inscP49 abdominal 2 dorsal acute muscle 3 inscP49 abdominal 2 dorsal oblique muscle 1 inscP49 abdominal 2 dorsal oblique muscle 4 inscP8 abdominal 2 dorsal oblique muscle 5 inscP49 abdominal 2 dorsal transverse muscle 1 inscP8 abdominal 2 lateral adult muscle precursor cell insc8 abdominal 2 lateral longitudinal muscle 1 insc3 abdominal 2 lateral pentascolopidial chordotonal organ lch5 insc3 abdominal 2 lateral transverse muscle 1 inscP8 abdominal 2 lateral transverse muscle 2 inscP8 abdominal 2 lateral transverse muscle 3 insc8 inscP8 abdominal 2 lch5 neuron | embryonic stage inscΔ13 abdominal 2 segment border muscle insc3 abdominal 2 ventral longitudinal muscle insc3 abdominal 2 ventral oblique muscle insc8 abdominal 2 ventral oblique muscle 1 insc3 abdominal 2 ventral oblique muscle 2 insc3 abdominal 2 ventral oblique muscle 3 insc3 abdominal 3 dorsal acute muscle 1 inscP49 abdominal 3 dorsal acute muscle 3 inscP49 abdominal 3 dorsal oblique muscle 1 inscP49 abdominal 3 dorsal oblique muscle 4 inscP8 abdominal 3 dorsal oblique muscle 5 inscP49 abdominal 3 dorsal transverse muscle 1 inscP8 abdominal 3 lateral adult muscle precursor cell insc8 abdominal 3 lateral longitudinal muscle 1 insc3 abdominal 3 lateral pentascolopidial chordotonal organ lch5 insc3 abdominal 3 lateral transverse muscle 1 inscP8 abdominal 3 lateral transverse muscle 2 inscP8 abdominal 3 lateral transverse muscle 3 insc8 inscP8 abdominal 3 lch5 neuron | embryonic stage inscΔ13 abdominal 3 segment border muscle insc3 abdominal 3 ventral longitudinal muscle insc3 abdominal 3 ventral oblique muscle insc8 abdominal 3 ventral oblique muscle 1 insc3 abdominal 3 ventral oblique muscle 2 insc3 abdominal 3 ventral oblique muscle 3 insc3 abdominal 4 dorsal acute muscle 1 inscP49 abdominal 4 dorsal acute muscle 3 inscP49 abdominal 4 dorsal oblique muscle 1 inscP49 abdominal 4 dorsal oblique muscle 4 inscP8 abdominal 4 dorsal oblique muscle 5 inscP49 abdominal 4 dorsal transverse muscle 1 inscP8 abdominal 4 lateral adult muscle precursor cell insc8 abdominal 4 lateral longitudinal muscle 1 insc3 abdominal 4 lateral pentascolopidial chordotonal organ lch5 insc3 abdominal 4 lateral transverse muscle 1 inscP8 abdominal 4 lateral transverse muscle 2 inscP8 abdominal 4 lateral transverse muscle 3 insc8 inscP8 abdominal 4 lch5 neuron | embryonic stage inscΔ13 abdominal 4 segment border muscle insc3 abdominal 4 ventral longitudinal muscle insc3 abdominal 4 ventral oblique muscle insc8 abdominal 4 ventral oblique muscle 1 insc3 abdominal 4 ventral oblique muscle 2 insc3 abdominal 4 ventral oblique muscle 3 insc3 abdominal 5 dorsal acute muscle 1 inscP49 abdominal 5 dorsal acute muscle 3 inscP49 abdominal 5 dorsal oblique muscle 1 inscP49 abdominal 5 dorsal oblique muscle 4 inscP8 abdominal 5 dorsal oblique muscle 5 inscP49 abdominal 5 dorsal transverse muscle 1 inscP8 abdominal 5 lateral adult muscle precursor cell insc8 abdominal 5 lateral longitudinal muscle 1 insc3 abdominal 5 lateral pentascolopidial chordotonal organ lch5 insc3 abdominal 5 lateral transverse muscle 1 inscP8 abdominal 5 lateral transverse muscle 2 inscP8 abdominal 5 lateral transverse muscle 3 insc8 inscP8 abdominal 5 lch5 neuron | embryonic stage inscΔ13 abdominal 5 segment border muscle insc3 abdominal 5 ventral longitudinal muscle insc3 abdominal 5 ventral oblique muscle insc8 abdominal 5 ventral oblique muscle 1 insc3 abdominal 5 ventral oblique muscle 2 insc3 abdominal 5 ventral oblique muscle 3 insc3 abdominal 6 dorsal acute muscle 1 inscP49 abdominal 6 dorsal acute muscle 3 inscP49 abdominal 6 dorsal oblique muscle 1 inscP49 abdominal 6 dorsal oblique muscle 4 inscP8 abdominal 6 dorsal oblique muscle 5 inscP49 abdominal 6 dorsal transverse muscle 1 inscP8 abdominal 6 lateral adult muscle precursor cell insc8 abdominal 6 lateral longitudinal muscle 1 insc3 abdominal 6 lateral pentascolopidial chordotonal organ lch5 insc3 abdominal 6 lateral transverse muscle 1 inscP8 abdominal 6 lateral transverse muscle 2 inscP8 abdominal 6 lateral transverse muscle 3 insc8 inscP8 abdominal 6 lch5 neuron | embryonic stage inscΔ13 abdominal 6 segment border muscle insc3 abdominal 6 ventral longitudinal muscle insc3 abdominal 6 ventral oblique muscle insc8 abdominal 6 ventral oblique muscle 1 insc3 abdominal 6 ventral oblique muscle 2 insc3 abdominal 6 ventral oblique muscle 3 insc3 abdominal 7 dorsal acute muscle 1 inscP49 abdominal 7 dorsal acute muscle 3 inscP49 abdominal 7 dorsal oblique muscle 1 inscP49 abdominal 7 dorsal oblique muscle 4 inscP8 abdominal 7 dorsal oblique muscle 5 inscP49 abdominal 7 dorsal transverse muscle 1 inscP8 abdominal 7 lateral adult muscle precursor cell insc8 abdominal 7 lateral longitudinal muscle 1 insc3 abdominal 7 lateral pentascolopidial chordotonal organ lch5 insc3 abdominal 7 lateral transverse muscle 1 inscP8 abdominal 7 lateral transverse muscle 2 inscP8 abdominal 7 lateral transverse muscle 3 insc8 inscP8 abdominal 7 lch5 neuron | embryonic stage inscΔ13 abdominal 7 segment border muscle insc3 abdominal 7 ventral longitudinal muscle insc3 abdominal 7 ventral oblique muscle insc8 abdominal 7 ventral oblique muscle 1 insc3 abdominal 7 ventral oblique muscle 2 insc3 abdominal 7 ventral oblique muscle 3 insc3 abdominal 8 dorsal oblique muscle 1 inscP49 abdominal 8 ventral longitudinal muscle insc3 abdominal 8 ventral oblique muscle insc8 abdominal adult muscle precursor cell insc8 abdominal anterior ventral multidendritic neuron vdaa | supernumerary insc22 abdominal v'esA neuron insc22 abdominal v'esB neuron insc22 abdominal ventral bipolar neuron vbp | supernumerary insc22 abdominal ventral multidendritic neuron | ectopic insc22 abdominal ventral sensillum campaniformium vc1 & thecogen cell insc22 abdominal ventral sensillum campaniformium vc2 & thecogen cell insc22 abdominal ventral sensillum campaniformium vc3 & thecogen cell insc22 abdominal ventral sensillum campaniformium vc4a & thecogen cell insc22 abdominal ventral sensillum campaniformium vc4b & thecogen cell insc22 abdominal vesA neuron insc22 abdominal vesB neuron insc22 abdominal vesC neuron insc22 aCC neuron | ectopic | somatic clone insc22 adult muscle precursor cell insc22 chordotonal organ inscP8 chordotonal organ | lateral insck02608 insck07505 insck07701 insck12405 dorsal oblique muscle inscP49 ectoderm & embryo & spindle insc3'DraIII.hs.T:Hsap\MYC insc3'RI.hs.T:Hsap\MYC insc5'NheI.hs.T:Zzzz\FLAG inscA1-A5.hs.T:Zzzz\FLAG inscA2-A3.hs.T:Zzzz\FLAG inscA2-A4.hs.T:Zzzz\FLAG inschs.PK EL neuron inscA2-A3.hs.T:Zzzz\FLAG inscA2-A4.hs.T:Zzzz\FLAG inscA3.hs.T:Zzzz\FLAG inscNLS.hs.T:Zzzz\FLAG embryonic/larval somatic muscle inscΔ13 insc22 inscP49 inscP8 embryonic epidermis & spindle, with Scer\GAL4h-1J3 inscΔ1.Scer\UAS.T:Hsap\MYC inscΔ2.Scer\UAS.T:Hsap\MYC inscΔ5.Scer\UAS.T:Hsap\MYC inscΔ6.Scer\UAS.T:Hsap\MYC inscΔC.Scer\UAS.T:Hsap\MYC inscCen.Scer\UAS.T:Ecol\lacZ inscCen.Scer\UAS.T:Hsap\MYC inscScer\UAS.T:Hsap\MYC embryonic neuroblast insc22 embryonic neuroblast & spindle insc22 external sensory organ precursor cell I, with Scer\GAL4neur-P72 inscScer\UAS.cKa ganglion mother cell GMC1-1a insc22 ganglion mother cell GMC4-2a insc22 hypodermal muscle of larval abdominal 9 insc3 insc8 inscP49 imaginal disc insck02517 Malpighian tubule tip cell | ectopic inscunspecified mesothoracic dorsal acute muscle 1 inscP49 mesothoracic dorsal oblique muscle 1 inscP49 mesothoracic lateral longitudinal muscle 1 insc3 inscP8 mesothoracic lateral transverse muscle insc3 mesothoracic segment border muscle insc3 mesothoracic ventral longitudinal muscle insc3 mesothoracic ventral oblique muscle insc8 metathoracic dorsal acute muscle 1 inscP49 metathoracic dorsal oblique muscle 1 inscP49 metathoracic lateral transverse muscle insc3 metathoracic segment border muscle insc3 metathoracic ventral longitudinal muscle insc3 metathoracic ventral oblique muscle insc8 metathoracic ventral oblique muscle 1 insc3 metathoracic ventral oblique muscle 2 insc3 metathoracic ventral oblique muscle 3 insc3 mitotic domain 9 & spindle insc22 neuroblast | P-stage, with Scer\GAL4sca-P309 inscScer\UAS.cKa neuroblast NB1-1 insc22 neuroblast NB1-1 | somatic clone insc22 neuroblast NB4-2 insc22 neuroblast NB4-2 | somatic clone insc22 neuroblast NB7-1 insc22 neuroblast NB7-1 | somatic clone insc22 pCC neuron insc22 pCC neuron | somatic clone insc22 pericardial cell | embryonic stage insc22 insc3 inscP49 inscP8 peripheral nervous system & spindle inscdsRNA.578 presumptive embryonic/larval central nervous system inscA2-A3.hs.T:Zzzz\FLAG inscA2-A4.hs.T:Zzzz\FLAG inscA3.hs.T:Zzzz\FLAG inscNLS.hs.T:Zzzz\FLAG presumptive embryonic/larval digestive system inscA2-A3.hs.T:Zzzz\FLAG inscA2-A4.hs.T:Zzzz\FLAG inscA3.hs.T:Zzzz\FLAG inscNLS.hs.T:Zzzz\FLAG presumptive embryonic/larval peripheral nervous system insck02608 insck07505 insck07701 insck12405 prothoracic dorsal oblique muscle 1 inscP49 RP2 motor neuron inscA2-A3.hs.T:Zzzz\FLAG inscA2-A4.hs.T:Zzzz\FLAG inscA3.hs.T:Zzzz\FLAG inscNLS.hs.T:Zzzz\FLAG RP2 motor neuron | ectopic | somatic clone insc22 RP2 motor neuron | embryonic stage insc22 RP2 motor neuron | supernumerary insc22 RP2sib neuron insc22 RP2sib neuron | embryonic stage insc22 RP2sib neuron | somatic clone insc22 scutellar bristle, with Scer\GAL4sca-537.4 inscEP2010 somatic muscle | dorsal insc22 insc8 somatic muscle | lateral insc8 somatic muscle | ventral insc22 insc8 tormogen cell, with Scer\GAL4sca-537.4 inscEP2010 trichogen cell, with Scer\GAL4sca-537.4 inscEP2010 trichogen cell | ectopic, with Scer\GAL4sca-537.4 inscEP2010 U neuron | somatic clone insc22 ventral midline neuroblast inscdsRNA.578 ventral multidendritic neuron vdaA | supernumerary insc22 ventral multidendritic neuron vdaB | supernumerary insc22 ventral multidendritic neuron vdaC | supernumerary insc22 ventral multidendritic neuron vdaD | supernumerary insc22 ventral nerve cord primordium, with Scer\GAL4sca-P309 inscScer\UAS.cKa wing inscAB44
Classical Alleles ( 34 )
For All Classical Alleles Show
Allele of insc Class Mutagen Stocks Known lesion inscP49 amorphic allele - genetic evidence, loss of function allele Delta2-3 2 Yes insc05475 P-element activity 2 Yes insck12405 P-element activity 2 Yes inscMz1407 2 Yes insc22 ethyl methanesulfonate 1 Yes insc8 ethyl methanesulfonate 1 -- inscd00468 1 -- inscd00869 P-element activity 1 -- inscDG24311 1 -- inscEY09668 P-element activity 1 -- inscf00771 1 -- insck02517 P-element activity 1 -- insck02608 P-element activity 1 Yes insck10907 P-element activity 1 -- insck13706 P-element activity 1 -- inscMI02180 1 -- inscMI05159 1 -- inscNP3201 1 -- inscNP4276 1 -- insc03679 P-element activity 0 -- insc3 ethyl methanesulfonate 0 -- inscAB44 P-element activity 0 Yes inscc06701 piggyBac transposase 0 -- inscd06672 P-element activity 0 -- inscDG02708 0 -- inscE70 P-element activity 0 Yes inscEP2010 P-element activity 0 Yes insck07505 P-element activity 0 Yes insck07701 P-element activity 0 Yes inscp17 loss of function allele 0 -- inscP43 0 -- inscP8 P-element activity 0 Yes inscunspecified 0 -- inscΔ13 Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 43 )
For All Alleles Carried on Transgenic Constructs Show
Allele of insc Class Mutagen Stocks Known lesion inscGD7267 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes inscHMS01456 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes inscHMS01819 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes inscHMS01883 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes inscKK110275 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes inscScer\UAS.cKa in vitro construct - regulatory fusion 1 Yes insc03.Scer\UAS.T:Ecol\lacZ in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insc04.Scer\UAS.T:Ecol\lacZ in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insc3'BsrBI.hs.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insc3'DraIII.hs.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insc3'NarI.hs.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insc3'NheI.hs.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insc3'RI.hs.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insc5'BsrBI.hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insc5'NheI.hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insc5'RI.hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscA1-A5.hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscA2-A3.hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscA2-A4.hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscA3.hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscBcII.hs.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscCDS.cHa in vitro construct 0 Yes inscCen.Scer\UAS.T:Ecol\lacZ in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscCen.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes insccHa in vitro construct 0 Yes inscdsRNA.578 hypomorphic allele - genetic evidence in vitro construct - RNAi 0 Yes inscdsRNA.cSa in vitro construct - RNAi 0 Yes inscdsRNA.cYa in vitro construct - RNAi 0 Yes inschs.PK in vitro construct - regulatory fusion 0 Yes inschs.PKb in vitro construct - regulatory fusion 0 Yes inscMtnA.PW in vitro construct - regulatory fusion 0 Yes inscNIG.11312R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes inscNLS.hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscScer\UAS.cen.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscScer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes inscΔ1.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes inscΔ2.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes inscΔ3.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes inscΔ4.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes inscΔ5.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes inscΔ6.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes inscΔC.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC404 (Christensen et al., 2007.10.29) Df(2R)BSC702 (Christensen et al., 2008.12.28) Df(2R)bwVDe2LPxKR (Kania et al., 1995) Df(2R)D4 (Kraut, 2000.8.14, Kraut and Campos-Ortega, 1996) Df(2R)E2 (Kraut, 2000.8.14) Df(2R)inscP49 (Hassan et al., 1998, Knirr et al., 1997) Df(2R)nemP49 (Kraut and Campos-Ortega, 1996) Df(2R)P72 (Chia et al., 1997, Kraut and Campos-Ortega, 1996, Knirr et al., 1997) Df(2R)Pu-D17 (BDGP Project Members, 1994-1999, Kania et al., 1995)
Not disrupted in	Df(2R)AA21 (BDGP Project Members, 1994-1999, Kania et al., 1995) Df(2R)exu1 (BDGP Project Members, 1994-1999) Df(2R)exu2 (BDGP Project Members, 1994-1999) Df(2R)Pu-D17 (Kraut and Campos-Ortega, 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7267} NA P{KK110275} NA P{NIG.11312R} NA P{TRiP.HMS01456} NA P{TRiP.HMS01819} NA P{TRiP.HMS01883} NA P{UAS-insc.03.T:lacZ} NA P{UAS-insc.04.T:lacZ} NA P{UAS-insc.Cen.T:lacZ} NA P{UAS-insc.Cen.T:Myc} NA P{UAS-insc.K} NA P{UAS-insc.T:Myc} NA P{UAS-insc.Δ1.T:Myc} NA P{UAS-insc.Δ2.T:Myc} NA P{UAS-insc.Δ3.T:Myc} NA P{UAS-insc.Δ4.T:Myc} NA P{UAS-insc.Δ5.T:Myc} NA P{UAS-insc.Δ6.T:Myc} NA P{UAS-insc.ΔC.T:Myc} NA P{UAS-insc-cen-GFP} NA P{UAS-insc-GFP} NA heat-shock construct P{hs-insc.3'BsrBI} NA P{hs-insc.3'DraIII} NA P{hs-insc.3'NarI} NA P{hs-insc.3'NheI} NA P{hs-insc.3'RI} NA P{hs-insc.5'BsrBI} NA P{hs-insc.5'NheI} NA P{hs-insc.5'RI} NA P{hs-insc.A1-A5} NA P{hs-insc.A2-A3} NA P{hs-insc.A2-A4} NA P{hs-insc.A3} NA P{hs-insc.BcII} NA P{hs-insc.K} NA P{hs-insc.Kb} NA P{hs-insc.NLS} NA reporter construct P{UAS-insc.03.T:lacZ} No P{UAS-insc.04.T:lacZ} No P{UAS-insc.Cen.T:lacZ} No
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}insck02517 No P{lacW}insck02608 No P{lacW}insck07505 No P{lacW}insck07701 No P{lacW}insck10907 No P{lacW}insck12405 No P{lacW}insck13706 No P{lacW}l(2)SH0227SH0227 No P{lacZ}inscAB44 No P{PZ}insc03679 No P{PZ}insc05475 No insertion of mobile activating element P{EP}inscEP2010 NA P{EPgy2}inscEY09668 NA P{XP}inscd00468 NA P{XP}inscd00869 NA P{XP}inscd06672 NA insertion of enhancer trap binary system P{GawB}inscMz1407 No P{GawB}inscNP3201 No P{GawB}inscNP4276 No miscellaneous insertions Mi{MIC}inscMI02180 NA Mi{MIC}inscMI05159 NA P{hsneo}inscP8 NA P{wHy}inscDG02708 NA P{wHy}sktlDG24311 NA PBac{PB}inscc06701 NA PBac{WH}inscf00771 NA
Gene Ontology: Function, Process & Cellular Component ( 24 unique terms )
Terms Based on Experimental Evidence ( 17 terms )
Molecular Function
CV term Evidence References
cytoskeletal adaptor activity inferred from mutant phenotype AND inferred from sequence or structural similarity (Kraut and Campos-Ortega, 1996) protein binding inferred from physical interaction with corn (Bulgheresi et al., 2001)
Biological Process
CV term Evidence References
apical protein localization inferred from mutant phenotype (Bulgheresi et al., 2001) inferred from physical interaction with corn (Bulgheresi et al., 2001) asymmetric neuroblast division inferred from genetic interaction with G-iα65A AND inferred from genetic interaction with raps (Cai et al., 2003) defense response to Gram-negative bacterium inferred from mutant phenotype (Berkey et al., 2009) establishment of mitotic spindle localization inferred from mutant phenotype (Kraut et al., 1996, Kaltschmidt et al., 2000) Malpighian tubule tip cell differentiation inferred from mutant phenotype (Wan et al., 2000) neuroblast fate determination inferred from direct assay (Udolph et al., 2009) peripheral nervous system development inferred from mutant phenotype (Burchard et al., 1995) protein localization inferred from mutant phenotype (Kraut et al., 1996) regulation of asymmetric cell division inferred from mutant phenotype (Gaziova and Bhat, 2009) regulation of nervous system development inferred from mutant phenotype (Gaziova and Bhat, 2009) RNA localization inferred from mutant phenotype (Li et al., 1997) somatic muscle development inferred from mutant phenotype (Burchard et al., 1995)
Cellular Component
CV term Evidence References
apical cortex inferred from direct assay (Kraut et al., 1996, Li et al., 1997, Siegrist and Doe, 2005, Nipper et al., 2007, Fuerstenberg et al., 1998) cell cortex inferred from direct assay (Akiyama-Oda et al., 2000) cytoplasm inferred from direct assay (Li et al., 1997)
Terms Based on Predictions or Assertions ( 13 terms )
Molecular Function
CV term Evidence References
cytoskeletal adaptor activity non-traceable author statement (Kraut, 1994.12.1)
Biological Process
CV term Evidence References
asymmetric cell division traceable author statement (Cheah et al., 2000) asymmetric neuroblast division traceable author statement (Li et al., 1997, Schaefer and Knoblich, 2001) asymmetric protein localization traceable author statement (Cheah et al., 2000) asymmetric protein localization involved in cell fate determination non-traceable author statement (Cayouette and Raff, 2002) traceable author statement (Schaefer and Knoblich, 2001) basal protein localization traceable author statement (Peng et al., 2000) cytoskeleton organization non-traceable author statement (Kraut et al., 1995) establishment of mitotic spindle localization non-traceable author statement (Li et al., 1997, Cayouette and Raff, 2002) traceable author statement (Cheah et al., 2000) neuroblast fate determination traceable author statement (Cheah et al., 2000) protein localization non-traceable author statement (Li et al., 1997) sensory organ development traceable author statement (Chen and Fishman, 2000)
Cellular Component
CV term Evidence References
apical cortex traceable author statement (Ohshiro et al., 2000, Bulgheresi et al., 2001) apical part of cell non-traceable author statement (Peng et al., 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
insc - mira pull down, autoradiography (Shen et al., 1998) insc - raps x-ray crystallography, pull down, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot (Culurgioni et al., 2011, Mauser and Prehoda, 2012)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions insc allele Gene References insc22 pdm2 (Bhat and Apsel, 2004) raps (Yu et al., 2003) inscEP2010 H (Abdelilah-Seyfried et al., 2000) N (Abdelilah-Seyfried et al., 2000) pnr (Pena-Rangel et al., 2002) inschs.PKb egl (Hughes et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63001 DroID - A comprehensive database of gene and protein interactions. • FBgn0011674 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37355
Orthologs
OrthoDB Orthologs (21) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6P8G3J Dipteran inclusive ortholog search EOG6NVZTF Insect inclusive ortholog search EOG6KKWXX Arthropod inclusive ortholog search EOG6P5HR8 Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6P8G3J)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\insc     Drosophila simulans   Dsim\GD25250 Y   Drosophila sechellia   Dsec\GM15774 Y   Drosophila erecta   Dere\GG20829 Y   Drosophila yakuba   Dyak\GE13768 Y   Drosophila ananassae   Dana\GF12742 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10909 Y   Drosophila persimilis   Dper\GL17423 Y   Drosophila willistoni   Dwil\GK22882 Y   Drosophila virilis   Dvir\GJ18662 Y   Drosophila mojavensis   Dmoj\GI19832 Y   Drosophila grimshawi   Dgri\GH19883 Y
Orthologs in non-Drosophila Dipterans (EOG6NVZTF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004719   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ020015   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001960
Orthologs in non-Dipteran Insects (EOG6KKWXX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Acyrthosiphon pisum Pea aphid  Apim\ACYPI008908   Bombyx mori Silkmoth  Bmor\BGIBMGA009407   Pediculus humanus Human body louse  Phum\PHUM123940   Tribolium castaneum Red flour beetle  Tcas\TC001320
Orthologs in non-Insect Arthropods (EOG6P5HR8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_310452   Ixodes scapularis Deer tick  Isca\ISCW003918
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD25250 Drosophila sechellia Dsec\GM15774 Drosophila erecta Dere\GG20829 Drosophila yakuba Dyak\GE13768 Drosophila ananassae Dana\GF12742 Drosophila pseudoobscura pseudoobscura Dpse\GA10909 Drosophila persimilis Dper\GL17423 Drosophila willistoni Dwil\GK22882 Drosophila virilis Dvir\GJ18662 Drosophila mojavensis Dmoj\GI19832 Drosophila grimshawi Dgri\GH19883
Stocks & Reagents
Stocks Listed in FlyBase ( 29 )
Bloomington	39677 insc8/CyO 39678 insc22/CyO 5849 inscP49/CyO 39676 w*; P{UAS-insc.K}3 8751 w*; P{GawB}inscMz1407 38351 y1 v1; P{TRiP.HMS01819}attP40
Harvard	- P{XP}inscd00468 - P{XP}inscd00869 - PBac{WH}inscf00771
Kyoto	108467 inscP49/CyO
VDRC	v102387 P{KK110275}VIE-260B v31488 w1118; P{GD7267}v31488
	More stocks available...
Genomic Clones ( 2 )
	BACR04E05 BACR10P16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 29 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE60102
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE28823 RE67442 SD04331
Other clones	101508 214371.74 214435.68 CK01.4C.G2 CK01.36A.F8 CK01.87A.G7 CK01.105B.G10 CK01121 EK068628 EK091606 EK227917 EP18562 EP21459 RP001007545 RP001015119 RP001022799 RP001029546 RP001030550 RP001034720 RP001040813 RP001044884 RP001045011 RP001050660 RP001052109 RP001056210
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011674 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37355
Antibody Information
	polyclonal (Kraut and Campos-Ortega, 1996) polyclonal antibody (Bhat and Apsel, 2004)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	The complementation group previously identified as 'fam' corresponds to the sktl/insc locus. Until it is demonstrated to be otherwise, all alleles previously represented as alleles of 'fam' are now recorded as alleles of both insc and sktl.
Other Comments
	insc has a role in augmenting metaphase spindle length in embryonic neuroblasts. (Hughes et al., 2004) insc regulates the apical-basal polarity of the pIIb division in the vp1-vp4a lineages in the embryo. (Orgogozo et al., 2001) mira and insc are required for the correct cortical location of pros protein in NB6-4T. (Akiyama-Oda et al., 2000) The central third of the insc coding region is sufficient for the normal glia-neuron cell-fate choice of the NB6-4T daughter cell. (Akiyama-Oda et al., 2000) Embryos deficient for insc function show failure of spindle rotation in neuroblasts. (Kaltschmidt et al., 2000) raps and insc might orient asymmetric cell divisions by localising or locally modulating a heterotrimeric G-protein signalling cascade at the apical cell cortex. (Schaefer et al., 2000) Apical localisation of insc protein in embryonic neuroblasts requires baz. (Schober et al., 1999) Localisation of insc protein depends on baz. (Wodarz et al., 1999) The correct positioning of numb protein and proper orientation of division in ganglion mother cells requires insc. (Buescher et al., 1998) Asymmetrical segregation of numb protein into one of the sibling cells produced by division of muscle progenitor cells depends on insc, and is essential for the specification of distinct sibling cell fates. (Carmena et al., 1998) Extrinsic and intrinsic cues and microfilaments regulate the subcellular localisation of the pros, insc and stau proteins in neuroblasts. (Broadus and Doe, 1997) insc and stau mediate asymmetric localization and segregation of pros RNA during neuroblast cell divisions. insc nucleates an apical complex and is required for protein localization, spindle orientation and RNA localization. (Li et al., 1997) Mutant embryos show lack of muscles without disturbance of myoblast fusion or epidermis pattern. (Buttgereit, 1996) Orientation of the mitotic spindle and correct localisation of numb and pros in basal daughter cells requires insc. (Kraut et al., 1996) Ectopic expression of insc in other epithelial cells leads to spindle reorientation. (Kraut et al., 1996) insc is a neural precursor gene and encodes a candidate for a cytoskeletal adaptor protein. (Kraut and Campos-Ortega, 1996) insc may be required for the development of the somatic musculature and the chordotonal organs of the PNS, but it is not involved in the development of the visceral mesoderm and the dorsal vessel. (Burchard et al., 1995) The CNS and PNS of insc mutants are morphologically abnormal, resulting from general disorganization of the neuroblast array and defective neuroblast morphology and abnormal sense organ morphology. The numbers of particular neuroblasts, and their ganglion mother cell progeny, are also faulty. (Kraut et al., 1995) The insc product may affect cytoskeletal organization and/or asymmetric division of neuronal precursors. (Kraut et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057676 RefSeq (Proteins) • NP_477024 DDBJ / EMBL / GenBank • AAB18232 AAF46626 AAX33399 AC004433 AQ074114 BT021251 CAA58175 CL632195 CZ470932 CZ471852 CZ475270 CZ475271 G00585 U73489 X83111 Entrez Gene - A searchable database of RefSeq genes. • 37355 UniProtKB/TrEMBL • Q5BIH3 Q9W2R4 Q24367 Q94543
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Armadillo-type fold (IPR016024) Armadillo-like helical (IPR011989) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • PC00157
Linkouts
	BioGRID - A database of protein and genetic interactions • 63001 DroID - A comprehensive database of gene and protein interactions. • FBgn0011674 DRSC - Results from RNAi screens. • FBgn0011674 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011674 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11312-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011674 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37355 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/inscutbl.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37355 modMine - Data generated by the modENCODE project. • FBgn0011674
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	25/17 (Roch et al., 1998) CG11312 (Keleman et al., 2009.8.5, Haussmann et al., 2008, Ni et al., 2011.7.19, Ni et al., 2010.12.1) fam (Kania et al., 1995, BDGP Project Members, 1994-1999, ) Ins (Ohno, 2001) insc (FlyBase Curators, 2008, Wang et al., 2006, Emery et al., 2005, Ahringer, 2005, Siegrist and Doe, 2005, Basto et al., 2006, Popichenko and Paululat, 2004, Griffiths et al., 2007, Christensen et al., 2007.10.29, Yu et al., 2002, Tio et al., 2011, Desroches et al., 2010, Kankel et al., 2007, Zhu and Bhat, 2011, Southall and Brand, 2009, Zhu and Bhat, 2011, Christensen et al., 2008.12.28, Bowman et al., 2008, Choksi et al., 2006, Egger et al., 2007, Tögel et al., 2008, Speicher et al., 2008, Sellin et al., 2009, Liu et al., 2009, Gaziova and Bhat, 2009, Rath et al., 2002, Zeitlinger et al., 2007, Udolph et al., 2009, Smith and Prehoda, 2011, Japanese National Institute of Genetics, 2012.5.21, Andersen et al., 2012) Insc (Speicher et al., 2007, Wang et al., 2006, Lee et al., 2006, Wang et al., 2007, Krahn and Wodarz, 2008, Yamashita et al., 2005, Wang et al., 2005, Chia et al., 2008, Siegrist and Doe, 2006, Nipper et al., 2007, Slack et al., 2007, Sousa-Nunes et al., 2009, Wang et al., 2009, Wang et al., 2011, Wirtz-Peitz et al., 2008, Wirtz-Peitz et al., 2008, Berkey et al., 2009, van Eyk et al., 2011, Yoshiura et al., 2012, Andersen et al., 2012) l(2)05475 (Chen et al., 1998, ) l(2)k12405 (Chen et al., 1998) nem (Knirr, 1996.10.6, Knirr et al., 1997, Li et al., 1997, Buttgereit, 1996, Kraut et al., 1996, Burchard et al., 1995, )
Name Synonym	fata morgana   inscrutable (Anonymous, 1997) Inscutable (Tepass, 2002) Inscuteable (Huwe et al., 2000, Yu et al., 2002, Yamashita et al., 2005, Hampoelz et al., 2005, Chia et al., 2008, Slack et al., 2007, Izumi et al., 2006, Speicher et al., 2008, Slack et al., 2006, Berkey et al., 2009, Ceron et al., 2006, Yoshiura et al., 2012) inscuteable (Kraut, 1994.12.1, Wang et al., 2006, Fuerstenberg et al., 1998, Tio et al., 2011, Desroches et al., 2010, Kankel et al., 2007, Zhu and Bhat, 2011, Choksi et al., 2006, Tögel et al., 2008, Gaziova and Bhat, 2009, Rath et al., 2002, Udolph et al., 2009, Johansson et al., 2005) not enough muscles
Secondary FlyBase IDs
	FBgn0010601 FBgn0013468 FBgn0015387
References ( 297 )
Generate a list of	All references relating to this gene ( 297 )
List References by type	Research paper  ( 129 ) Supplementary material  ( 8 ) Review  ( 67 ) Erratum  ( 2 ) Personal communication to FlyBase  ( 18 ) Abstract  ( 63 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 ) Letter  ( 1 ) Conference report  ( 1 )
Recent research papers ( 11 )
	Andersen et al., 2012, Dev. Biol. 363(1): 258--265 Sgt1 acts via an LKB1/AMPK pathway to establish cortical polarity in larval neuroblasts. [FBrf0217488] Mauser and Prehoda, 2012, PLoS ONE 7(1): e29611 Inscuteable regulates the Pins-Mud spindle orientation pathway. [FBrf0218437] Yoshiura et al., 2012, Dev. Cell 22(1): 79--91 Tre1 GPCR Signaling Orients Stem Cell Divisions in the Drosophila Central Nervous System. [FBrf0217269] Culurgioni et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(52): 20998--21003 Inscuteable and NuMA proteins bind competitively to Leu-Gly-Asn repeat-enriched protein (LGN) during asymmetric cell divisions. [FBrf0217064] Smith and Prehoda, 2011, Mol. Cell 43(4): 540--549 Robust spindle alignment in Drosophila neuroblasts by ultrasensitive activation of pins. [FBrf0214708] Tio et al., 2011, PLoS ONE 6(11): e26879 Asymmetric cell division and notch signaling specify dopaminergic neurons in Drosophila. [FBrf0216651] van Eyk et al., 2011, Hum. Mol. Genet. 20(14): 2783--2794 Perturbation of the Akt/Gsk3-{beta} signalling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs. [FBrf0213942] Viktorin et al., 2011, Dev. Biol. 356(2): 553--565 Multipotent neural stem cells generate glial cells of the central complex through transit amplifying intermediate progenitors in Drosophila brain development. [FBrf0214495] Wang et al., 2011, Dev. Cell 21(3): 520--533 An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts. [FBrf0215580] Zhu and Bhat, 2011, Dev. Biol. 357(2): 283--294 The Hem protein mediates neuronal migration by inhibiting WAVE degradation and functions opposite of Abelson tyrosine kinase. [FBrf0214765] Zhu and Bhat, 2011, Mech. Dev. 128(7-10): 483--495 The Drosophila Hem/Kette/Nap1 protein regulates asymmetric division of neural precursor cells by regulating localization of Inscuteable and Numb. [FBrf0216763] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...