FlyBase Gene Report: Dmel\pav

dorsal head epidermis primordium

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | intense | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | intense | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

organism | intense | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

insulin secreting cell of pars intercerebralis

(Fisher et al., 2012)

embryonic neuroblast of ventral nerve cord primordium

(Fisher et al., 2012)

germline cell

(Fisher et al., 2012)

procephalic neuroblast

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain glial cell

(Fisher et al., 2012)

embryonic central brain surface glia

(Fisher et al., 2012)

germline cell

(Fisher et al., 2012)

gonad

(Fisher et al., 2012)

sensory nervous system primordium

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

ventral sensory complex primordium

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

egg chamber

(Minestrini et al., 2002)

nurse cell

• nucleus

(Minestrini et al., 2002)

ring canal

(Minestrini et al., 2002)

adult stage | female & oogenesis stage S7

oocyte

• nucleus

(Minestrini et al., 2002)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic cycle 16

• midbody

Comment: telophase

(Somers and Saint, 2003)

• spindle

Comment: metaphase

(Somers and Saint, 2003)

• spindle of cell cortex

Comment: anaphase

(Somers and Saint, 2003)

adult stage

egg chamber

(Minestrini et al., 2002)

nurse cell

• nucleus

(Minestrini et al., 2002)

ring canal

(Minestrini et al., 2002)

adult stage | female & oogenesis stage S7

oocyte

• nucleus

(Minestrini et al., 2002)

• spindle

Comment: metaphase; expression assayed in cultured S2 cells

(Somers and Saint, 2003)

• midbody

Comment: anaphase-telophase; expression assayed in cultured S2 cells

telophase; expression assayed in cultured S2 cells

(Somers and Saint, 2003, Somma et al., 2002)

• cytoplasm

Comment: metaphase; expression assayed in cultured S2 cells

(Somma et al., 2002)

• nucleus

Comment: interphase; expression assayed in cultured S2 cells

(Somers and Saint, 2003)

View Dmel\pav in GBrowse 2

• spindle of cell cortex

Comment: expression assayed in cultured S2 cells; anaphase

(Somers and Saint, 2003)

Additional Descriptive Data

Expression of the protein is detected in the early egg chamber in the ring canals of the follicle cells and the oocyte and starting in stage 7 oocytes the protein isdetected in the oocyte nuclei. Lower levels of the protein are also detected in nurse cell nuclei. A pav-GFP protein confirms this localization although accumulation was more extensive and observed earlier in oogenesis, likely as a consequence of higher levels of expression mediated by the transgenic construct.

(Minestrini et al., 2002, Adams et al., 1998)

In S2 cells the pavprotein is found in a diffuse pattern throughout the cytoplasm during methaphase but concentrates at the central spindle microtubules in anaphase and telophase.

(Somma et al., 2002)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axoneme

inferred from direct assay

(Carmena et al., 1998)

part_of centralspindlin complex

inferred from direct assay

(Montembault et al., 2010)

located_in cleavage furrow

inferred from direct assay

(Sechi et al., 2017, Carmena et al., 1998)

located_in contractile ring

inferred from direct assay

(Carmena et al., 1998)

located_in female germline ring canal

inferred from direct assay

(Airoldi et al., 2011)

located_in kinetochore microtubule

inferred from direct assay

(Delcros et al., 2006)

located_in male germline ring canal

inferred from direct assay

(Carmena et al., 1998)

located_in midbody

inferred from direct assay

(Minestrini et al., 2003)

located_in mitotic spindle midzone

inferred from direct assay

(Derivery et al., 2015, Goshima and Vale, 2005)

located_in nucleus

inferred from direct assay

(Jones et al., 2010, Goshima and Vale, 2005, Minestrini et al., 2003)

located_in somatic ring canal

inferred from direct assay

(Airoldi et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\pav

View exonic expression by tissue for Dmel\pav

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011692

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38515/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011692

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011692

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011692

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1258

Flygut - An atlas of the Drosophila adult midgut

FBgn0011692

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\pav

Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of pav

Transgenic constructs containing regulatory region of pav

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene disrupted in

Df(3L)BSC370

(Christensen et al., 2007.10.29)

Df(3L)ems13

(Salzberg et al., 1994)

Df(3L)Exel9000

(Ryder, 2004.4.26)

Df(3L)GN24

(Jones et al., 2010, Collins and Cohen, 2005, Szeged Stock Center, 1998-)

Df(3L)GN50

(Unhavaithaya et al., 2013, Collins and Cohen, 2005, Szeged Stock Center, 1998-, Deak et al., 1997)

Df(3L)TE1

(Salzberg et al., 1994)

Gene not disrupted in

Df(3L)GN19

(Collins and Cohen, 2005, Salzberg et al., 1994)

Df(3L)ZN47

(Collins and Cohen, 2005)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

pav^GD7765

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

pav^GD7765

lethal (with Df(3L)GN50)

lethal | recessive

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

pav^GD7765

lethal - all die during embryonic stage | recessive

viable, with Scer\GAL4^pnr-MD237

pav^GD7765

viable, with Scer\GAL4^tin.CΔ4

pav^GD7765

viable, with Scer\GAL4^{VP16.mat.αTub67C}

Sterility

Allele

female fertile

pav^{Ubi-p63E.mGFP6}

female sterile, with Scer\GAL4^{VP16.mat.αTub67C}

fertile, with Scer\GAL4^{VP16.mat.αTub67C}

Other Phenotypes

Allele

abnormal cytokinesis | embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

pav^{DEAD.UAS.mGFP6}

abnormal cytokinesis | embryonic stage | recessive

abnormal cytokinesis | somatic clone

pav^B200

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PR

pav^GD7765

abnormal meiotic cell cycle | dominant

pav^Scim

abnormal mitotic cell cycle

pav^B200

abnormal neuroanatomy | embryonic stage | recessive

pav^3C53

abnormal neuroanatomy | larval stage

pav^GD7765

abnormal size | embryonic stage | somatic clone

decreased cell death | larval stage | somatic clone

pav^B200

decreased occurrence of cell division | embryonic stage

pav^S093413

decreased occurrence of cell division | embryonic stage | somatic clone

increased cell size | embryonic stage

pav^S093413

increased cell size | larval stage | somatic clone

pav^B200

some die during embryonic stage | recessive

visible, with Scer\GAL4^pnr-MD237

pav^GD7765

wild-type

pav^+t10.5

Phenotype manifest in

Allele

abdominal ventral denticle belt | embryonic stage

pav^B200

abdominal ventral denticle belt | embryonic stage (with Df(3L)GN24)

pav^B200

actin filament & nurse cell, with Scer\GAL4^{VP16.mat.αTub67C}

pav^{NLS4-7*.UASp.mGFP6}

centrosome | embryonic cycle 16

egg, with Scer\GAL4^{VP16.mat.αTub67C}

pav^UASp.mGFP6

egg chamber, with Scer\GAL4^{VP16.mat.αTub67C}

embryonic/larval somatic muscle cell

pav^B200

embryonic epidermis

pav^3C53

ganglion mother cell | larval stage

pav^GD7765

larval longitudinal connective

pav^unspecified

larval neuroblast | larval stage

pav^GD7765

larval ventral nerve cord | embryonic stage

pav^3C53

mesothoracic tergum, with Scer\GAL4^pnr-MD237

pav^GD7765

microtubule

pav^B200

mitotic domain 1 | embryonic cycle 16

mushroom body & axon | somatic clone

pav^B200

neuron & peripheral nervous system

neuron | embryonic stage

pav^S093413

nucleus

pav^3C53

nucleus (with Df(3L)GN50)

pav^3C53

nurse cell, with Scer\GAL4^{VP16.mat.αTub67C}

pav^UASp.mGFP6

nurse cell & microtubule, with Scer\GAL4^{VP16.mat.αTub67C}

pav^{NLS4-7*.UASp.mGFP6}

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

presumptive embryonic/larval central nervous system

pav^unspecified

presumptive embryonic/larval peripheral nervous system

pav^S093413

ring canal, with Scer\GAL4^{VP16.mat.αTub67C}

pav^{NLS4-7*.UASp.mGFP6}

spindle | embryonic cycle 16

symmetrical commissure

pav^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\KIF23

14 of 15

Yes

Hsap\KIF20A

3 of 15

Hsap\KIF20B

3 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Kif23

12 of 15

Yes

Mmus\Kif20a

3 of 15

Mmus\Kif20b

3 of 15

Rattus norvegicus (Norway rat) (3)

Rnor\Kif23

9 of 13

Yes

Rnor\Kif20a

2 of 13

Rnor\Kif20b

2 of 13

Xenopus tropicalis (Western clawed frog) (4)

Xtro\kif23

9 of 12

Yes

Xtro\kif20a

2 of 12

Xtro\kif20b

1 of 12

Yes

Xtro\LOC100489788

1 of 12

Danio rerio (Zebrafish) (5)

Drer\kif23

14 of 15

Yes

3 of 15

2 of 15

2 of 15

2 of 15

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\zen-4

14 of 15

Yes

Cele\klp-10

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\klp9

7 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (26)

Gene Symbol

Score

Source

Alignment

sub

5 of 10

cana

2 of 10

CG32318

2 of 10

cmet

2 of 10

cos

2 of 10

Khc-73

2 of 10

Khc

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

ncd

2 of 10

neb

2 of 10

nod

2 of 10

unc-104

2 of 10

CG5004

1 of 10

CG14535

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KIF23; kinesin family member 23

14 of 15

605064

KIF20A; kinesin family member 20A

3 of 15

605664

CARDIOMYOPATHY, FAMILIAL RESTRICTIVE, 6; RCM6

KIF20B; kinesin family member 20B

3 of 15

605498

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

cosedimentation through density gradient, molecular weight, proximity ligation assay, fluorescence microscopy, proximity-dependent biotin identification, Identification by mass spectrometry, western blot

(Mannix et al., 2019, Sommi et al., 2010)

pav - ALiX

pull down, autoradiography, anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Lie-Jensen et al., 2019)

pav - AP-2μ

(Guruharsha et al., 2011)

pav - Arf51F

two hybrid, pull down, western blot

(Dyer et al., 2007)

pav - BicD

(Guruharsha et al., 2011)

pav - CG32344

proximity-dependent biotin identification, Identification by mass spectrometry

(Guruharsha et al., 2011)

pav - CG43333

(Mannix et al., 2019)

pav - CG8939

(Guruharsha et al., 2011)

pav - Dgp-1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Guruharsha et al., 2011)

pav - Lkb1

(Vinayagam et al., 2016)

pav - Map205

(Guruharsha et al., 2011)

pav - Srp72

(Guruharsha et al., 2011)

pav - eIF2β

pull down, autoradiography

(Guruharsha et al., 2011)

pav - feo

(Bassi et al., 2013)

pav - foxo

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

pav - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

pav - l(1)G0020

pull down, autoradiography

(Guruharsha et al., 2011)

pav - neb

(Bassi et al., 2013)

pav - nesd

pull down, autoradiography, anti tag coimmunoprecipitation, peptide massfingerprinting

(Montembault et al., 2010)

pav - pit

two hybrid, pull down, western blot, anti tag coimmunoprecipitation, molecular weight estimation by staining

(Guruharsha et al., 2011)

pav - sau

(Sechi et al., 2020, Sechi et al., 2014)

pav - scra

anti tag coimmunoprecipitation, peptide massfingerprinting

(D'Avino et al., 2008)

pav - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

pav - sti

pull down, autoradiography, anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Bassi et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

pav|tum

enhanceable

(Jones et al., 2010)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Arf51F

suppressible

(Marcetteau et al., 2021)

arm

suppressible

(Jones et al., 2010)

nesd

enhanceable

(Montembault et al., 2010)

nesd

suppressible

(Montembault et al., 2010)

pbl

enhanceable

(Gregory et al., 2007)

smo

enhanceable

(Collins, 2005.7.7, Collins and Cohen, 2005)

smo

suppressible

(Collins, 2005.7.7, Collins and Cohen, 2005)

tum

enhanceable