Pav-KLP, 2A12, Pavarotti-KLP, Pavarotti KLP, Mklp1
a kinesin motor protein - required both to establish the structure of the telophase spindle to provide a framework for assembly of the contractile ring and to mobilize mitotic regulator proteins - Pavarotti as an inhibitor of kinesin-1-driven microtubule sliding that regulates neurite outgrowth
Please see the JBrowse view of Dmel\pav for information on other features
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Gene model reviewed during 5.46
There is only one protein coding transcript and one polypeptide associated with this gene
886 (aa); 100 (kD observed)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pav using the Feature Mapper tool.
Comment: maternally deposited
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Expression of the protein is detected in the early egg chamber in the ring canals of the follicle cells and the oocyte and starting in stage 7 oocytes the protein isdetected in the oocyte nuclei. Lower levels of the protein are also detected in nurse cell nuclei. A pav-GFP protein confirms this localization although accumulation was more extensive and observed earlier in oogenesis, likely as a consequence of higher levels of expression mediated by the transgenic construct.
In S2 cells the pavprotein is found in a diffuse pattern throughout the cytoplasm during methaphase but concentrates at the central spindle microtubules in anaphase and telophase.
GBrowse - Visual display of RNA-Seq signals
View Dmel\pav in GBrowse 23-10
3-10
3-9.2
3-18--20
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for identity of: pav CG1258
Source for merge of: pav Mklp1
pav regulates microtubule organisation during myogenesis.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in spindle shape and cytokinesis failure when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: binucleate cells.
Five EMS induced alleles have been identified in a screen for mutations affecting commissure formation in the CNS of the embryo.
Candidate gene for quantitative trait (QTL) locus determining bristle number.
pav is required to organise the central spindle and contractile ring for cytokinesis.
Quantifying rates of protein sequence divergence within and between species reveals that the Drosophila genome harbors a substantial proportion of genes with a very high divergence rate.
Mutation in pav affects cell division in the neuronal lineage.
pav is required for PNS development in the embryo.