RK2, reverse polarity
Gene model reviewed during 5.54
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\repo using the Feature Mapper tool.
Comment: reported as glioblasts of ventral nervous system
Comment: reported as dorsal/lateral sensory complexes
repo is first expressed in embryonic stage 9 in glioblasts located in the lateral-most row of neuroblasts and at the anterior margin of each segment. The repo-expressing cell divides symmetrically to produce progeny. A third repo-positive cell is seen medial to the first pair in stage 11. In stage 12, two clusters of stained cells flank each segment border. By germ band retraction, numerous longitudinal glia are stained in every hemisegment. On northern blots, the highest levels of repo transcripts are observed in embryos and adult heads.
pupal expression assayed at 12 hr APF
Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d7
At late stage 10 and into stage 11 repo protein is detected in approximately 20 glial cells or glioblasts in the proto and trito cerebral hemispheres.
In the embryonic CNS, all known glial cells express repo protein, with the exception of the midline glia and two of three segmental nerve root glial cells. In the CNS of a stage 16 embryo, 27-29 cells per hemimere are stained. In the PNS, all known peripheral glia express repo protein except for the glia associated with the transverse nerve. In addition, some ligament cells, and the lateral bipolar dendrite neuron express repo protein. repo expression appear to be glial specific in the brain as well.
repo protein is expressed throughout development. It is expressed in all glia of the developing PNS and CNS, excluding the midline glia. It is first detected in embryonic stage 11 in a single cell per hemisegment, which corresponds to the lateral glioblast. These cells divide to form two repo-positive cells.These cells subsequently divide and migrate to form the longitudinal glial array. Many other repo-positive cells (presumably of neuroblast origin) appear and will ultimately contribute to the perineurium surrounding the CNS. By stage 12, most of the repo-expressing cells are evident. They include exit glia, three peripheral glial cells per hemisegment (ps1,2,3) and ligament cells of the chordotonal organs. Double staining for repo and elav shows that the repo-postivie cells are glial. repo is also expressed in glial cells in the imaginal discs. In the eye disc, repo is expressed in the glial cells from the optic lobes which populate the optic stalk and subretinal region. repo is also found associated with axon tracts in the leg and wing imaginal discs.
GBrowse - Visual display of RNA-Seq signalsView Dmel\repo in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
repo is required for the differentiation and maintenance of glia function in the embryonic nervous system, but not for the determination of the glial cells.
repo gene product is not required for the initial determination of glial cells but it is essential for their further differentiation and for the maintenance of glial function.
Neurons in the laminar region of the repo optic lobe undergo cell death, indicating that laminar glia express one or more factors essential for neuronal survival. Also repo photoreceptor cells degenerate following cell death of the laminar cells. This retinal degeneration in repo appears to be due to retrograde degeneration.
Encodes the glial specific epitope for the antibody RK2 of A. Tomlinson.