FB2020_06, released Dec 22, 2020

Gene: Dmel\Syx5

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General Information
Symbol
Dmel\Syx5
Species
D. melanogaster
Name
Syntaxin 5
Annotation Symbol
CG4214
Feature Type
protein_coding_gene
FlyBase ID
FBgn0011708
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Snapshot
Syntaxin 5 (Syx5) encodes a type II transmembrane protein in the Q-SNARE family that forms hetero tetramers required for membrane function in the early secretory pathway (ER and Golgi). It has been involved in cytokinesis during spermatogenesis. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

Sed5, l(2)35Ff, Syntaxin, BG:DS02740.12 , dSyx5

Key Links
Genomic Location
Cytogenetic map
35F1-35F1
Sequence location
2L:16,307,346..16,309,274 [+]
Recombination map

2-52

RefSeq locus
NT_033779 REGION:16307346..16309274
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (26 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables protein homodimerization activity
inferred from physical interaction with UniProtKB:Q24509
(assigned by UniProt )
(Banfield et al., 1994)
enables SNAP receptor activity
inferred from physical interaction with FLYBASE:αSnap; FB:FBgn0250791
(Xu et al., 2002)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables SNAP receptor activity
inferred from biological aspect of ancestor with PANTHER:PTN000461349
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity
(Littleton, 2000)
enables SNARE binding
inferred from biological aspect of ancestor with PANTHER:PTN000461349
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence model
(Xu et al., 2002)
Biological Process (15 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
involved_in cell morphogenesis
inferred from mutant phenotype
(D'Ambrosio and Vale, 2010)
involved_in cellular copper ion homeostasis
inferred from mutant phenotype
(Norgate et al., 2010)
involved_in copper ion homeostasis
inferred from mutant phenotype
(Norgate et al., 2007)
involved_in exocytosis
inferred from mutant phenotype
(Xu et al., 2002)
involved_in larval lymph gland hemopoiesis
inferred from mutant phenotype
(Mondal et al., 2014)
involved_in male meiosis cytokinesis
inferred from mutant phenotype
(Xu et al., 2002)
involved_in protein secretion
inferred from mutant phenotype
(D'Ambrosio and Vale, 2010)
involved_in spermatogenesis
inferred from mutant phenotype
(Xu et al., 2002)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport
inferred from biological aspect of ancestor with PANTHER:PTN000461791
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in intracellular protein transport
inferred from biological aspect of ancestor with PANTHER:PTN000461349
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in neurotransmitter secretion
non-traceable author statement
(Lloyd et al., 2000)
involved_in synaptic vesicle docking
inferred from sequence or structural similarity
(Littleton, 2000)
involved_in vesicle docking
inferred from biological aspect of ancestor with PANTHER:PTN000461349
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in vesicle fusion
inferred from biological aspect of ancestor with PANTHER:PTN000461349
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in vesicle-mediated transport
non-traceable author statement
(Lloyd et al., 2000)
Cellular Component (8 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in cis-Golgi network
inferred from direct assay
(assigned by UniProt )
(Banfield et al., 1994)
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in Golgi apparatus
inferred from direct assay
(Xu et al., 2002)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
located_in endomembrane system
inferred from biological aspect of ancestor with PANTHER:PTN000461349
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in endoplasmic reticulum
inferred from sequence or structural similarity with UniProtKB:Q08851
(assigned by UniProt )
(UniProt Curators, 2002-)
located_in Golgi membrane
inferred from biological aspect of ancestor with PANTHER:PTN000461791
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with UniProtKB:Q08851
(assigned by UniProt )
(UniProt Curators, 2002-)
located_in integral component of membrane
inferred from biological aspect of ancestor with PANTHER:PTN000461349
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in plasma membrane
inferred from sequence or structural similarity
(Littleton, 2000)
part_of SNARE complex
inferred from biological aspect of ancestor with PANTHER:PTN000461349
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence model
(Xu et al., 2002)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the syntaxin family. (Q24509)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
Qa-SNAREs -
Q-SNAREs are members of the SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein (SNAP) receptor) family that mediate vesicle-fusion. SNAREs are cytoplasmic, membrane-anchored proteins that contain a 60 amino acid SNARE domain. Three Q-SNAREs domains from the target membrane and one R-SNARE domain from the vesicle interact to form a four four-helix bundle that pulls the two membranes in close proximity and promotes fusion. After fusion, the complexes are dissembled by NSF (N-ethylmaleimide-sensitive factor) and SNAPs (Soluble NSF Attachment Proteins). Q-SNAREs are named from their contribution of a conserved glutamine (Q) residue to the helix bundle. Q-SNARE domains are designated a, b or c, depending on their position within the helix bundle. (Adapted from PMID:15893389 and FBrf0216182).
Protein Function (UniProtKB)
Mediates endoplasmic reticulum to Golgi transport.
(UniProt, Q24509)
Summary (Interactive Fly)

a Golgi-localized SNARE protein - required for ER-Golgi traffic and Golgi reassembly following cell division - required for steps leading to formation of mature sperm

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Syx5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Syx5-RA
FBtr0080832
NM_134305
1861
467
Syx5-RB
FBtr0080831
NM_078858
1768
467
Additional Transcript Data and Comments
Reported size (kB)

1.0 (northern blot)

(Roth et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Syx5-PA
FBpp0080390
51.8
467
6.53
NP_599132
AAF53520
Syx5-PB
FBpp0080389
51.8
467
6.53
NP_523582
AAN10935
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

467 aa isoforms: Syx5-PA, Syx5-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

310 (aa)

(Banfield et al., 1994)
Comments
External Data
Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q24509)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Syx5 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Roth et al., 1995)
adult stage
(Roth et al., 1995)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cis-Golgi network
inferred from direct assay
(assigned by UniProt )
(Banfield et al., 1994)
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in Golgi apparatus
inferred from direct assay
(Xu et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Syx5 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Syx5
Transgenic constructs containing regulatory region of Syx5
Deletions and Duplications ( 60 )
Disrupted in
Df(2L)Exel7065
(Ryder, 2004.4.26)
Df(2L)H60-3
(Ashburner et al., 1999, Roote, 1996.1.11, Roote, 1996.8, Dawson et al., 1995)
Df(2L)k08106-rv6
(Roote, 1999.7.7)
Df(2L)k08106-rv15
(Roote, 1999.7.7)
Df(2L)k14608-mr29
(Roote, 2000.6.13)
Df(2L)r10
(Ashburner et al., 1990)
Df(2L)RA5
(Eberl et al., 1998, Ashburner et al., 1990)
Df(2L)RM5
(Ashburner et al., 1990)
Df(2L)RN2
(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)
Df(2L)TE35D-16
(Ashburner et al., 1990)
Df(2L)TE35D-21
(Dawson et al., 1995, Ashburner et al., 1990)
Df(2L)TE35D-23
(Ashburner et al., 1990)
Not disrupted in
Df(2L)64j
(Ashburner et al., 1990)
Df(2L)A72
(Ashburner et al., 1990)
Df(2L)A267
(Ashburner et al., 1990)
Df(2L)ARR1
(Ashburner et al., 1990)
Df(2L)b80e4
(Ashburner et al., 1990)
Df(2L)cact-255rv64
(Dawson et al., 1995)
Df(2L)el20
(Ashburner et al., 1990)
Df(2L)el28
(Ashburner et al., 1990)
Df(2L)fn12
(Ashburner et al., 1990)
Df(2L)fn26
(Ashburner et al., 1990)
Df(2L)fn27
(Ashburner et al., 1990)
Df(2L)H20
(Ashburner et al., 1990)
Df(2L)III18
(Ashburner et al., 1999, Roth et al., 1995)
Df(2L)n78l3
(Ashburner et al., 1990)
Df(2L)noc11
(Ashburner et al., 1990)
Df(2L)osp29
(Eberl et al., 1998)
Df(2L)osp38
(Ashburner et al., 1990)
Df(2L)rd9
(Ashburner et al., 1990)
Df(2L)Sco7
(Ashburner et al., 1990)
Df(2L)Scorv16
(Ashburner et al., 1990)
Df(2L)Scorv18
(Ashburner et al., 1990)
Df(2L)Scorv19
(Ashburner et al., 1990)
Df(2L)Scorv25
(Ashburner et al., 1990)
Df(2L)SS1
(Ashburner et al., 1990)
Df(2L)TE35BC-3
(Ashburner et al., 1990)
Df(2L)TE35BC-4
(Ashburner et al., 1990)
Df(2L)TE35BC-34
(Ashburner et al., 1990)
Df(2L)TE35D-1
(Ashburner et al., 1990)
Df(2L)TE35D-2
(Ashburner et al., 1990)
Df(2L)TE35D-3
(Ashburner et al., 1990)
Df(2L)TE35D-4
(Ashburner et al., 1990)
Df(2L)TE35D-6
(Ashburner et al., 1990)
Df(2L)TE35D-13
(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)
Df(2L)TE35D-14
(Eberl et al., 1998, Ashburner et al., 1990)
Df(2L)TE35D-15
(Ashburner et al., 1990)
Df(2L)TE35D-19
(Ashburner et al., 1990)
Df(2L)TE35D-20
(Ashburner et al., 1990)
Df(2L)TE35D-22
(Ashburner et al., 1990)
In(2L)dpps22Ldppd36R
(Ashburner et al., 1990)
In(2L)el18
(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)
In(2LR)Scorv1-DV7
(Ashburner et al., 1990)
T(2;3)Scorv7
(Ashburner et al., 1990)
Duplicated in
Dp(2;3)osp3
(Ashburner et al., 1990)
Not duplicated in
Dp(2;1)Scorv23
(Ashburner et al., 1990)
Dp(2;2)GYL
(Ashburner et al., 1990)
Dp(2;2)TE35B-54L
(Ashburner et al., 1990)
In(2L)C163.41LC158R
(Ashburner et al., 1990)
In(2L)Scorv17LC158R
(Ashburner et al., 1990)
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4elav.PLu
lethal, with Scer\GAL4Mef2.PR
lethal, with Scer\GAL4pnr-MD237
lethal | larval stage, with Scer\GAL4elav.PU
lethal | larval stage, with Scer\GAL4GMR.PFa
lethal | larval stage, with Scer\GAL4mex1.2.1
lethal | P-stage, with Scer\GAL4elav.PU
lethal | P-stage, with Scer\GAL4GMR.PFa
lethal | P-stage, with Scer\GAL4mex1.2.1
lethal | recessive
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal - majority die (with Syx5AR113), with Scer\GAL4- :
partially lethal - majority die (with Syx5EP2313), with Scer\GAL4- :
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
male sterile (with Syx5AR113), with Scer\GAL4- :
male sterile (with Syx5EP2313), with Scer\GAL4- :
Other Phenotypes
Allele
chemical resistant | dominant
cytokinesis defective (with Syx5AR113), with Scer\GAL4- :
cytokinesis defective (with Syx5EP2313), with Scer\GAL4- :
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
visible, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4pnr-MD237, Syx5GD1743
visible, with Scer\GAL4pnr-MD237, Syx5UAS.Tag:MYC
wild-type, with Scer\GAL4da.G32
Phenotype manifest in
Allele
adult cuticle, with Scer\GAL4pnr-MD237
adult cuticle, with Scer\GAL4pnr-MD237, Syx5GD1743
adult cuticle, with Scer\GAL4pnr-MD237, Syx5UAS.Tag:MYC
chaeta, with Scer\GAL4pnr-MD237
embryonic/larval lymph gland, with Scer\GAL4HCH.Hand, Scer\GAL4Hml.Δ
embryonic/larval salivary gland, with Scer\GAL4fkh.PH
endoplasmic reticulum, with Scer\GAL4Cg.PU
endoplasmic reticulum | adult stage
fat body, with Scer\GAL4Cg.PU
lipid droplet | adult stage
Nebenkern (with Syx5AR113), with Scer\GAL4- :
Nebenkern (with Syx5EP2313), with Scer\GAL4- :
photoreceptor cell
rhabdomere
scutellum, with Scer\GAL4pnr-MD237
spermatozoon (with Syx5AR113), with Scer\GAL4- :
spermatozoon (with Syx5EP2313), with Scer\GAL4- :
testis (with Syx5AR113), with Scer\GAL4- :
testis (with Syx5EP2313), with Scer\GAL4- :
thorax, with Scer\GAL4pnr-MD237
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\STX5
14 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Stx5a
13 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Stx5
12 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\stx5
13 of 12
Yes
Yes
Danio rerio (Zebrafish) (2)
Drer\stx5a
14 of 15
Yes
Yes
Drer\stx5al
8 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\syx-5
14 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\SYP32
9 of 9
Yes
Yes
Atha\SYP31
6 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\SED5
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\sed5
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909PS )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00001756
Drosophila simulans
Dsim\GD24051
Drosophila sechellia
Dsec\GM18666
Drosophila erecta
Dere\GG25202
Drosophila yakuba
Dyak\GE21453
Drosophila ananassae
Dana\GF14580
Drosophila pseudoobscura pseudoobscura
Dpse\GA18038
Drosophila persimilis
Dper\GL16332
Drosophila willistoni
Dwil\GK23986
Drosophila virilis
Dvir\GJ10629
Drosophila mojavensis
Dmoj\GI17075
Drosophila grimshawi
Dgri\GH10314
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091508CS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17015160
Glossina morsitans
Tsetse fly
Gmor\GMOY003075
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_10165
Mayetiola destructor
Hessian fly
Mdes\Mdes004473
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL013541
Anopheles darlingi
American malaria mosquito
Adar\ADAC001755
Anopheles gambiae
Malaria mosquito
Agam\AGAP008756
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ003088
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0BA6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA010620
Heliconius melpomene
Postman butterfly
Hmel\HMEL016426
Apis florea
Little honeybee
Aflo\2369932452
Apis mellifera
Western honey bee
Amel\LOC552117
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP20823
Bombus terrestris
Buff-tailed bumblebee
Bter\15208457
Linepithema humile
Argentine ant
Lhum\LH15505
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002680
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG007331
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_01460
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_03018
Pediculus humanus
Human body louse
Phum\PHUM351020
Rhodnius prolixus
Kissing bug
Rpro\RPRC012736
Cimex lectularius
Bed bug
Clec\CLEC006517
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001052
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04718
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0B7V )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR012910
Ixodes scapularis
Black-legged tick
Isca\ISCW024059
Stegodyphus mimosarum
African social velvet spider
Smim\KFM74134.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur11g04240
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_41202
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0HR8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Stx5
Ciona intestinalis
Vase tunicate
Cint\LOC100181871
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (8)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Syx1A
2 of 10
Syx4
2 of 10
Syx7
2 of 10
Syx13
2 of 10
Syx16
2 of 10
Syx17
2 of 10
Syx6
1 of 10
Syx8
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    STX5; syntaxin 5
    14 of 15
    603189
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Syx5
        comigration in non denaturing gel electrophoresis, anti tag western blot
        (Banfield et al., 1994)
        Syx5 - Bet1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Gos28
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Membrin
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Nsf2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Sec20
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Sec22
        anti tag coimmunoprecipitation, anti tag western blot, experimental knowledge based
        (Zhao et al., 2015, Guruharsha et al., 2011)
        Syx5 - Slh
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Snap24
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Snap25
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Snap29
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Syb
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Syx13
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Syx16
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Syx18
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Syx1A
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Syx7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Syx8
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Use1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Vamp7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Vti1a
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - Ykt6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - koko
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - αSnap
        pull down, western blot, experimental knowledge based, two hybrid
        (Guruharsha et al., 2011, Xu et al., 2002)
        Syx5 - γSnap1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Syx5 - γSnap2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Syx5
        enhanceable
        Ctr1A
        (Norgate et al., 2010)
        Syx5
        enhanceable
        Sec22
        (Zhao et al., 2015)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Nsf2
        enhanceable
        Syx5
        (Xu et al., 2002)
        ru
        suppressible
        Syx5
        (Yogev et al., 2010)
        External Data
        Subunit Structure (UniProtKB)
        Homodimer.
        (UniProt, Q24509 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map

        2-52

        Cytogenetic map
        35F1-35F1
        Sequence location
        2L:16,307,346..16,309,274 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        35F1-35F1
        Limits computationally determined from genome sequence between P{lacW}heixk11403&P{lacW}heixk12401 and P{EP}fzyEP1028&P{lacW}mRpL4k14608
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        35F-35F
        (Lloyd et al., 2000)
        35F6-35F12
        (Dawson et al., 1995)
        35D3-36A7
        (determined by in situ hybridisation)
        (BDGP Project Members, 1994-1999)
        Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.
        (Lloyd et al., 2000)
        Experimentally Determined Recombination Data
        Location

        2-52

        (FlyBase, 2016)

        2-49.7

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (10)
        Genomic Clones (12)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (118)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: Sed5 CG4214

          (Zhong, 1999.11)

          Source for identity of: Syx5 Sed5

          (McLennan, 2001.5.22)
          Source for database merge of

          Source for merge of: Sed5 l(2)35Ff anon-35Fd anon-35Fb

          (Ashburner et al., 1999)
          Additional comments
          Other Comments

          S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

          (Stroschein-Stevenson et al., 2006)

          dsRNA made from templates generated with primers directed against this gene was tested in an RNAi screen to identify genes that regulate Ca2+ release.

          (Zhang et al., 2006)

          Identified in an RNAi screen for host factors that alter infection of SL2 cells by L.monocytogenes.

          (Agaisse et al., 2005)

          dsRNA made from templates generated with primers directed against Syx5 that is transfected into S2 treated with Listeria monocytogenes reveals Syx5 to be involved in Listeria monocytogenes entry.

          (Cheng et al., 2005)

          dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

          (Echard et al., 2004)

          dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

          (Kiger et al., 2003)

          Syx5 is required for the proper function of the Golgi apparatus, and as a consequence required for cytokinesis and spermatid differentiation.

          (Xu et al., 2002)

          Transcript identified during molecular analysis of the fzy genomic region.

          (Dawson et al., 1995)

          Identified as a transcript in the cni region.

          (Roth et al., 1995)

          Studies of Syx5 expressed in COS cells indicates the location of Syx5 is only partially determined by its transmembrane domain and an additional localisation mechanism(s) must exist.

          (Banfield et al., 1994)

          1 allele is discussed but is not named.

          (Ashburner et al., 1990)
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 46 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (31)
          Reported As
          Symbol Synonym
          BG:DS02740.12
          (Ashburner et al., 1999, Ashburner et al., 1999.10.12)
          CG4214
          (Hosono et al., 2015, Keleman et al., 2009.8.5, Perkins et al., 2009, Dorer et al., 2006, Dunne et al., 2002, Verstreken and Bellen, 2000.9.4)
          DmSyx5
          (McLennan, 2001.5.22, Littleton, 2000)
          II
          (Dawson et al., 1995)
          SED5
          (Lloyd et al., 2000, Lewis, 1994.3.18)
          STX5
          (Gordon et al., 2017)
          SYN5
          (Dorer et al., 2006)
          Sed5
          (Bolshakov et al., 2002, Whitfield et al., 2002, Dunne and Rabouille, 2001, Gaasterland et al., 2000, Reese et al., 2000, Reese et al., 2000, Ashburner et al., 1999, Ashburner et al., 1999, Weimbs et al., 1997, Roth et al., 1995)
          Syntaxin 5
          (Giansanti et al., 2012, Edwards et al., 2006)
          Synx5
          (Bard et al., 2006)
          Syx
          (Wagh et al., 2006)
          Syx5
          (Hall et al., 2019, Xu et al., 2019, Ng and Jackson, 2015, Zhao et al., 2015, Mondal et al., 2014, Cruz-Garcia et al., 2013, Aikin et al., 2012, Fabian and Brill, 2012, Japanese National Institute of Genetics, 2012.5.21, D'Ambrosio and Vale, 2010, Norgate et al., 2010, Rousset et al., 2010, Tan et al., 2009, Kloepper et al., 2008, Dietzl et al., 2007, Norgate et al., 2007, Stroschein-Stevenson et al., 2006)
          Syx5A
          (Cheng et al., 2005)
          anon-35Fb
          anon-35Fd
          dSed5
          (Banfield et al., 1994)
          dSed5p
          (Kondylis and Rabouille, 2003)
          dSyx5
          (Xu et al., 2002, Xu et al., 2002)
          dsed5
          (Dunne et al., 2002)
          l(2)35Ff
          (Roote, 2000.6.13, Ashburner et al., 1999, Eberl et al., 1998, Roote, 1996.1.11, Roote, 1995.5.4, Spradling et al., 1995, Ashburner et al., 1990)
          sed5
          (Yogev et al., 2010, Bellen, 2000.12.25)
          syn5
          (Kondylis et al., 2011)
          syx5
          (González-Méndez et al., 2020, Lesch and Page, 2012, Ricardo and Lehmann, 2009, Cherry et al., 2006, Willingham and Keil, 2004)
          Name Synonyms
          Syntaxin
          (Hossain et al., 2008, Kittel et al., 2006, Pereanu and Hartenstein, 2006, Wagh et al., 2006)
          Syntaxin 5
          (Saadin and Starz-Gaiano, 2018, Schopf and Huber, 2017, Aikin et al., 2012, Norgate et al., 2010, Rousset et al., 2010, Januschke et al., 2007, Edwards et al., 2006, Zhang et al., 2006, McLennan, 2001.5.22)
          Syntaxin5
          (Zhao et al., 2015)
          dSyntaxin5
          (Schotman et al., 2008)
          dsyntaxin5
          (Schotman et al., 2008)
          lethal(2)35Ff
          syntaxin
          (Dumstrei et al., 2003)
          syntaxin 5
          (Coutinho-Budd et al., 2017, Gokhale et al., 2015, Lesch and Page, 2012, Maybeck and Röper, 2009, Ricardo and Lehmann, 2009)
          syntaxin5
          (Cruz-Garcia et al., 2013, Kondylis et al., 2011)
          Secondary FlyBase IDs
          • FBgn0001996
          • FBgn0015339
          • FBgn0020602
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (116)