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Gene Dmel\Syx5

General Information
Symbol	Dmel\Syx5	Species	D. melanogaster
Name	Syntaxin 5	Annotation symbol	CG4214
Feature type	protein_coding_gene	FlyBase ID	FBgn0011708
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	Sed5, l(2)35Ff, dSyx5, dsed5, DmSyx5, BG:DS02740.12, SYN5
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	35F1-35F1	Sequence location	2L:16,307,346..16,309,274 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Syntaxin 5 is referred to in FlyBase by the symbol Dmel\Syx5 (CG4214, FBgn0011708). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein homodimerization activity. There is experimental evidence that it is involved in the biological process: cellular copper ion homeostasis; Golgi organization; regulation of cell diameter; cell proliferation; negative regulation of mitotic cell cycle; cytokinesis; protein secretion; cell morphogenesis; phagocytosis, engulfment. 15 alleles are reported. The phenotypes of these alleles are annotated with: Golgi stack; Nebenkern; adult cuticle; chaeta; testis; thorax; endoplasmic reticulum; spermatozoon; scutellum. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Syntaxin, N-terminal; Syntaxin/epimorphin, conserved site; Target SNARE coiled-coil domain; t-SNARE. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult crop, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, adult spermathecae, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult midgut, larval/adult hindgut, adult heart, larval trachea, adult ovary, adult testis, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1625607_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Syx5. Gene sequence location is 2L:16307346..16309274.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms cellular copper ion homeostasis
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 35F1-35F1   Limits computationally determined from genome sequence between P{lacW}heixk11403&P{lacW}heixk12401 and P{EP}fzyEP1028&P{lacW}mRpL4k14608
Experimentally Determined Cytological Location
Cytogenetic map Notes References 35F6-35F12     (Dawson et al., 1995) 35D3-36A7   (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999) 35F-35F   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Syx5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48 Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Syx5-RA FBtr0080832  NM_134305   1861   467 Syx5-RB FBtr0080831  NM_078858   1768   467
Additional Transcript Data & Comments
Reported size (kB)	1.0 (northern blot) (Roth et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Syx5-PA   FBpp0080390   51.8   467   6.53     NP_599132   AAF53520 Syx5-PB   FBpp0080389   51.8   467   6.53     NP_523582   AAN10935
Additional Polypeptide Data & Comments
Reported size (kDa)	310 (aa) (Banfield et al., 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Target SNARE coiled-coil domain (IPR000727) Syntaxin, N-terminal (IPR006011) Syntaxin/epimorphin, conserved site (IPR006012) t-SNARE (IPR010989)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202437   AA390846   AA803006   AA949231   AA952027   AE014134 AAF53520   AE014134 AAN10935   AI256882   AI257090   AI258682   AI294931   AI296625   AI402800   AI532336   AI541895   AW944530   AY069832 AAL39977   BE976199   BF496084   BI162155   BI162156   BI172636   BI228883   BI234534   BI236693   BI361338   BI371876   BI482458   BI483043   BI569423   BI569424   BI605087   BI612208   BI623626   BP556970   BP556971   CK661499   CL525967   CO191001   CO275547   CO277185   CO326327   CO337675   CZ480421   EC062392   EC064608   EC069564   EC205903   EC243624   EC259020   EC261578   EV586288   EV589465   EV590843   GH751474   GH751585   GH751591   GH752513   GH756190   GH760652   GH773091   GH773517   GH782764   GH783722   GH784316   GH785582   GH792139   GH792421   GH792484   GH794180   GH797001   GH799132   GH804135   GH807692   GH808033   GH810296   GH810455   GH812586   GH812783   GH815112   GH816195   GH817134   GH818103   GH819284   GH828648   GH831860   GH832842   GH835349   GH836615   GH839796   GH841000   GH842169   GH842401   GH842630   GH848898   GH849632   GH852514   GH853861   GH858438   GH863223   GH864769   GH870998   GH871216   GH873913   GH876172   GH877801   GH882778   GH883646   GH886326   GH887542   GH888757   GH891934   GH898278   GH898727   GH902554   GH909686   GH910961   GH916530   GH917202   GH918111   GH920070   GH922857   GH925254   GH925807   GH926781   GH932219   GH933355   GH935996   GH937800   X78219 CAA55064
UniProtKB/Swiss-Prot	Q24509
UniProtKB/TrEMBL	A4V0S1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Roth et al., 1995) adult stage     (Roth et al., 1995)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cis-Golgi network inferred from direct assay (assigned by UniProtKB) (Banfield et al., 1994) Golgi apparatus inferred from direct assay (Xu et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult crop, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, adult spermathecae, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult midgut, larval/adult hindgut, adult heart, larval trachea, adult ovary, adult testis, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    247.275 Larval Midgut    469.1 Larval Hindgut    377.1 Larval Malpighian Tubules    633.9 Larval Fat Body    532.8 Larval Salivary Gland    1370.1 Larval Trachea    322.875 Larval Carcass    359.375 Adult Head    407.5 Adult Eye    418.675 Adult Brain    442 Adult Thoracic-Abdominal Ganglion    405.7 Adult Crop    504.3 Adult Midgut    422.9 Adult Hindgut    413.2 Adult Malpighian Tubules    670 Adult Fat Body    504.8 Adult Salivary Gland    1892.8 Adult Heart    403.7 Adult VirginFemale Spermatheca    632.1 Adult InseminatedFemale Spermatheca    629.9 Adult Ovary    496.8 Adult Testis    310 Adult Male Accessory Gland    1102.4 Adult Carcass    390.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    247.275 Larval Midgut    469.1 Larval Hindgut    377.1 Larval Malpighian Tubules  (633.9) Larval Fat Body  532.8 Larval Salivary Gland  (1370.1) Larval Trachea    322.875 Larval Carcass    359.375 Adult Head    407.5 Adult Eye    418.675 Adult Brain    442 Adult Thoracic-Abdominal Ganglion    405.7 Adult Crop  504.3 Adult Midgut    422.9 Adult Hindgut    413.2 Adult Malpighian Tubules  (670) Adult Fat Body  504.8 Adult Salivary Gland  (1892.8) Adult Heart    403.7 Adult VirginFemale Spermatheca  (632.1) Adult InseminatedFemale Spermatheca  (629.9) Adult Ovary    496.8 Adult Testis    310 Adult Male Accessory Gland  (1102.4) Adult Carcass    390.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    247.275 Larval Midgut    469.1 Larval Hindgut    377.1 Larval Malpighian Tubules    633.9 Larval Fat Body    532.8 Larval Salivary Gland  (1370.1) Larval Trachea    322.875 Larval Carcass    359.375 Adult Head    407.5 Adult Eye    418.675 Adult Brain    442 Adult Thoracic-Abdominal Ganglion    405.7 Adult Crop    504.3 Adult Midgut    422.9 Adult Hindgut    413.2 Adult Malpighian Tubules    670 Adult Fat Body    504.8 Adult Salivary Gland  (1892.8) Adult Heart    403.7 Adult VirginFemale Spermatheca    632.1 Adult InseminatedFemale Spermatheca    629.9 Adult Ovary    496.8 Adult Testis    310 Adult Male Accessory Gland  (1102.4) Adult Carcass    390.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    247.275 Larval Midgut    469.1 Larval Hindgut    377.1 Larval Malpighian Tubules    633.9 Larval Fat Body    532.8 Larval Salivary Gland    1370.1 Larval Trachea    322.875 Larval Carcass    359.375 Adult Head    407.5 Adult Eye    418.675 Adult Brain    442 Adult Thoracic-Abdominal Ganglion    405.7 Adult Crop    504.3 Adult Midgut    422.9 Adult Hindgut    413.2 Adult Malpighian Tubules    670 Adult Fat Body    504.8 Adult Salivary Gland    1892.8 Adult Heart    403.7 Adult VirginFemale Spermatheca    632.1 Adult InseminatedFemale Spermatheca    629.9 Adult Ovary    496.8 Adult Testis    310 Adult Male Accessory Gland    1102.4 Adult Carcass    390.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    247.275 Larval Midgut    469.1 Larval Hindgut    377.1 Larval Malpighian Tubules    633.9 Larval Fat Body    532.8 Larval Salivary Gland    1370.1 Larval Trachea    322.875 Larval Carcass    359.375 Adult Head    407.5 Adult Eye    418.675 Adult Brain    442 Adult Thoracic-Abdominal Ganglion    405.7 Adult Crop    504.3 Adult Midgut    422.9 Adult Hindgut    413.2 Adult Malpighian Tubules    670 Adult Fat Body    504.8 Adult Salivary Gland    1892.8 Adult Heart    403.7 Adult VirginFemale Spermatheca    632.1 Adult InseminatedFemale Spermatheca    629.9 Adult Ovary    496.8 Adult Testis    310 Adult Male Accessory Gland    1102.4 Adult Carcass    390.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    247.275 Larval Midgut    469.1 Larval Hindgut    377.1 Larval Malpighian Tubules  633.9 Larval Fat Body  532.8 Larval Salivary Gland  (1370.1) Larval Trachea    322.875 Larval Carcass    359.375 Adult Head    407.5 Adult Eye    418.675 Adult Brain    442 Adult Thoracic-Abdominal Ganglion    405.7 Adult Crop  504.3 Adult Midgut    422.9 Adult Hindgut    413.2 Adult Malpighian Tubules  670 Adult Fat Body  504.8 Adult Salivary Gland  (1892.8) Adult Heart    403.7 Adult VirginFemale Spermatheca  632.1 Adult InseminatedFemale Spermatheca  629.9 Adult Ovary    496.8 Adult Testis    310 Adult Male Accessory Gland  (1102.4) Adult Carcass    390.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    247.275 Larval Midgut    469.1 Larval Hindgut    377.1 Larval Malpighian Tubules    633.9 Larval Fat Body    532.8 Larval Salivary Gland  1370.1 Larval Trachea    322.875 Larval Carcass    359.375 Adult Head    407.5 Adult Eye    418.675 Adult Brain    442 Adult Thoracic-Abdominal Ganglion    405.7 Adult Crop    504.3 Adult Midgut    422.9 Adult Hindgut    413.2 Adult Malpighian Tubules    670 Adult Fat Body    504.8 Adult Salivary Gland  (1892.8) Adult Heart    403.7 Adult VirginFemale Spermatheca    632.1 Adult InseminatedFemale Spermatheca    629.9 Adult Ovary    496.8 Adult Testis    310 Adult Male Accessory Gland  1102.4 Adult Carcass    390.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    247.275 Larval Midgut    469.1 Larval Hindgut    377.1 Larval Malpighian Tubules    633.9 Larval Fat Body    532.8 Larval Salivary Gland    1370.1 Larval Trachea    322.875 Larval Carcass    359.375 Adult Head    407.5 Adult Eye    418.675 Adult Brain    442 Adult Thoracic-Abdominal Ganglion    405.7 Adult Crop    504.3 Adult Midgut    422.9 Adult Hindgut    413.2 Adult Malpighian Tubules    670 Adult Fat Body    504.8 Adult Salivary Gland    1892.8 Adult Heart    403.7 Adult VirginFemale Spermatheca    632.1 Adult InseminatedFemale Spermatheca    629.9 Adult Ovary    496.8 Adult Testis    310 Adult Male Accessory Gland    1102.4 Adult Carcass    390.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    407.5   NA  Eye    418.675   NA  Brain    442   247.275  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    405.7   NA  Crop    504.3   469.1  Midgut    422.9   377.1  Hindgut    413.2   633.9  Malpighian Tubules    670   532.8  Fat Body    504.8   1370.1  Salivary Gland    1892.8   NA  Heart    403.7   322.875  Trachea    NA   NA  VirginFemale Spermatheca    632.1   NA  InseminatedFemale Spermatheca    629.9   NA  Ovary    496.8   NA  Testis    310   NA  Male Accessory Gland    1102.4   359.375  Carcass    390.3 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    14 central nervous system, pupae P8    13 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    17 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    17 head, mated 1-day male    19 head, mated 4-day male    22 head, mated 20-day male    27 salivary gland, larvae L3 wandering    23 salivary gland, white prepupae    50 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    22 digestive system, 4-day adult    24 digestive system, 20-day adult    17 fat body, larvae L3 wandering    12 fat body, white prepupae    24 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    17 carcass, 4-day adult    19 carcass, 20-day adult    18 ovary, virgin 4-day female    40 ovary, mated 4-day female    34 testis, mated 4-day male    26 accessory gland, mated 4-day male    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (29) central nervous system, larvae L3    14 central nervous system, pupae P8    13 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    17 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    17 head, mated 1-day male    19 head, mated 4-day male    22 head, mated 20-day male  (27) salivary gland, larvae L3 wandering    23 salivary gland, white prepupae  (50) digestive system, larvae L3 wandering  (30) digestive system, 1-day adult    22 digestive system, 4-day adult    24 digestive system, 20-day adult    17 fat body, larvae L3 wandering    12 fat body, white prepupae    24 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    17 carcass, 4-day adult    19 carcass, 20-day adult    18 ovary, virgin 4-day female  (40) ovary, mated 4-day female  (34) testis, mated 4-day male  26 accessory gland, mated 4-day male  (42) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    14 central nervous system, pupae P8    13 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    17 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    17 head, mated 1-day male    19 head, mated 4-day male    22 head, mated 20-day male    27 salivary gland, larvae L3 wandering    23 salivary gland, white prepupae    50 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    22 digestive system, 4-day adult    24 digestive system, 20-day adult    17 fat body, larvae L3 wandering    12 fat body, white prepupae    24 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    17 carcass, 4-day adult    19 carcass, 20-day adult    18 ovary, virgin 4-day female    40 ovary, mated 4-day female    34 testis, mated 4-day male    26 accessory gland, mated 4-day male    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    14 central nervous system, pupae P8    13 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    17 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    17 head, mated 1-day male    19 head, mated 4-day male    22 head, mated 20-day male    27 salivary gland, larvae L3 wandering    23 salivary gland, white prepupae    50 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    22 digestive system, 4-day adult    24 digestive system, 20-day adult    17 fat body, larvae L3 wandering    12 fat body, white prepupae    24 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    17 carcass, 4-day adult    19 carcass, 20-day adult    18 ovary, virgin 4-day female    40 ovary, mated 4-day female    34 testis, mated 4-day male    26 accessory gland, mated 4-day male    42 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    14 central nervous system, pupae P8    13 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    17 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    17 head, mated 1-day male    19 head, mated 4-day male    22 head, mated 20-day male    27 salivary gland, larvae L3 wandering    23 salivary gland, white prepupae    50 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    22 digestive system, 4-day adult    24 digestive system, 20-day adult    17 fat body, larvae L3 wandering    12 fat body, white prepupae    24 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    17 carcass, 4-day adult    19 carcass, 20-day adult    18 ovary, virgin 4-day female    40 ovary, mated 4-day female    34 testis, mated 4-day male    26 accessory gland, mated 4-day male    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  29 central nervous system, larvae L3    14 central nervous system, pupae P8    13 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    17 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    17 head, mated 1-day male    19 head, mated 4-day male    22 head, mated 20-day male  27 salivary gland, larvae L3 wandering    23 salivary gland, white prepupae  (50) digestive system, larvae L3 wandering  30 digestive system, 1-day adult    22 digestive system, 4-day adult    24 digestive system, 20-day adult    17 fat body, larvae L3 wandering    12 fat body, white prepupae    24 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    17 carcass, 4-day adult    19 carcass, 20-day adult    18 ovary, virgin 4-day female  (40) ovary, mated 4-day female  (34) testis, mated 4-day male  26 accessory gland, mated 4-day male  (42) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    14 central nervous system, pupae P8    13 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    17 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    17 head, mated 1-day male    19 head, mated 4-day male    22 head, mated 20-day male    27 salivary gland, larvae L3 wandering    23 salivary gland, white prepupae    50 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    22 digestive system, 4-day adult    24 digestive system, 20-day adult    17 fat body, larvae L3 wandering    12 fat body, white prepupae    24 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    17 carcass, 4-day adult    19 carcass, 20-day adult    18 ovary, virgin 4-day female    40 ovary, mated 4-day female    34 testis, mated 4-day male    26 accessory gland, mated 4-day male    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    14 central nervous system, pupae P8    13 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    17 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    17 head, mated 1-day male    19 head, mated 4-day male    22 head, mated 20-day male    27 salivary gland, larvae L3 wandering    23 salivary gland, white prepupae    50 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    22 digestive system, 4-day adult    24 digestive system, 20-day adult    17 fat body, larvae L3 wandering    12 fat body, white prepupae    24 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    17 carcass, 4-day adult    19 carcass, 20-day adult    18 ovary, virgin 4-day female    40 ovary, mated 4-day female    34 testis, mated 4-day male    26 accessory gland, mated 4-day male    42 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    35 embryo 04-06hr    27 embryo 06-08hr    28 embryo 08-10hr    29 embryo 10-12hr    33 embryo 12-14hr    34 embryo 14-16hr    33 embryo 16-18hr    36 embryo 18-20hr    32 embryo 20-22hr    27 embryo 22-24hr    31 larva L1    30 larva L2    40 larva L3 12hr old    31 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    38 larva L3 puffstage 7-9    50 white prepupae new    62 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    44 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    42 adult male 05day    43 adult male 30day    46 adult female 01day    35 adult female 05day    51 adult female 30day    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (63) embryo 02-04hr  (35) embryo 04-06hr  (27) embryo 06-08hr  (28) embryo 08-10hr  (29) embryo 10-12hr  (33) embryo 12-14hr  (34) embryo 14-16hr  (33) embryo 16-18hr  (36) embryo 18-20hr  (32) embryo 20-22hr  (27) embryo 22-24hr  (31) larva L1  (30) larva L2  (40) larva L3 12hr old  (31) larva L3 puffstage 1-2  (31) larva L3 puffstage 3-6  (38) larva L3 puffstage 7-9  (50) white prepupae new  (62) white prepupae 12hr  (35) white prepupae 24hr  (37) pupae 2d postWPP  (44) pupae 3d postWPP  (32) pupae 4d postWPP  (38) adult male 01day  (42) adult male 05day  (43) adult male 30day  (46) adult female 01day  (35) adult female 05day  (51) adult female 30day  (50) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    35 embryo 04-06hr    27 embryo 06-08hr    28 embryo 08-10hr    29 embryo 10-12hr    33 embryo 12-14hr    34 embryo 14-16hr    33 embryo 16-18hr    36 embryo 18-20hr    32 embryo 20-22hr    27 embryo 22-24hr    31 larva L1    30 larva L2    40 larva L3 12hr old    31 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    38 larva L3 puffstage 7-9    50 white prepupae new    62 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    44 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    42 adult male 05day    43 adult male 30day    46 adult female 01day    35 adult female 05day    51 adult female 30day    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    35 embryo 04-06hr    27 embryo 06-08hr    28 embryo 08-10hr    29 embryo 10-12hr    33 embryo 12-14hr    34 embryo 14-16hr    33 embryo 16-18hr    36 embryo 18-20hr    32 embryo 20-22hr    27 embryo 22-24hr    31 larva L1    30 larva L2    40 larva L3 12hr old    31 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    38 larva L3 puffstage 7-9    50 white prepupae new    62 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    44 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    42 adult male 05day    43 adult male 30day    46 adult female 01day    35 adult female 05day    51 adult female 30day    50 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    35 embryo 04-06hr    27 embryo 06-08hr    28 embryo 08-10hr    29 embryo 10-12hr    33 embryo 12-14hr    34 embryo 14-16hr    33 embryo 16-18hr    36 embryo 18-20hr    32 embryo 20-22hr    27 embryo 22-24hr    31 larva L1    30 larva L2    40 larva L3 12hr old    31 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    38 larva L3 puffstage 7-9    50 white prepupae new    62 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    44 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    42 adult male 05day    43 adult male 30day    46 adult female 01day    35 adult female 05day    51 adult female 30day    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (63) embryo 02-04hr  (35) embryo 04-06hr  27 embryo 06-08hr  28 embryo 08-10hr  29 embryo 10-12hr  (33) embryo 12-14hr  (34) embryo 14-16hr  (33) embryo 16-18hr  (36) embryo 18-20hr  32 embryo 20-22hr  27 embryo 22-24hr  31 larva L1  30 larva L2  (40) larva L3 12hr old  31 larva L3 puffstage 1-2  31 larva L3 puffstage 3-6  (38) larva L3 puffstage 7-9  (50) white prepupae new  (62) white prepupae 12hr  (35) white prepupae 24hr  (37) pupae 2d postWPP  (44) pupae 3d postWPP  32 pupae 4d postWPP  (38) adult male 01day  (42) adult male 05day  (43) adult male 30day  (46) adult female 01day  (35) adult female 05day  (51) adult female 30day  (50) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    35 embryo 04-06hr    27 embryo 06-08hr    28 embryo 08-10hr    29 embryo 10-12hr    33 embryo 12-14hr    34 embryo 14-16hr    33 embryo 16-18hr    36 embryo 18-20hr    32 embryo 20-22hr    27 embryo 22-24hr    31 larva L1    30 larva L2    40 larva L3 12hr old    31 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    38 larva L3 puffstage 7-9    50 white prepupae new    62 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    44 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    42 adult male 05day    43 adult male 30day    46 adult female 01day    35 adult female 05day    51 adult female 30day    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    35 embryo 04-06hr    27 embryo 06-08hr    28 embryo 08-10hr    29 embryo 10-12hr    33 embryo 12-14hr    34 embryo 14-16hr    33 embryo 16-18hr    36 embryo 18-20hr    32 embryo 20-22hr    27 embryo 22-24hr    31 larva L1    30 larva L2    40 larva L3 12hr old    31 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    38 larva L3 puffstage 7-9    50 white prepupae new    62 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    44 pupae 3d postWPP    32 pupae 4d postWPP    38 adult male 01day    42 adult male 05day    43 adult male 30day    46 adult female 01day    35 adult female 05day    51 adult female 30day    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    26 embryonic 1182-4H    24 embryonic GM2    27 embryonic Kc167    37 embryonic S1    33 embryonic S3    25 leg disc CME L1    36 wing disc CME-W2    22 wing disc ML-DmD8    15 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    28 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    34 ovary fGS/OSS    37 ovary OSC    23 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (39) Schneider line 2 Sg4  26 embryonic 1182-4H    24 embryonic GM2  (27) embryonic Kc167  (37) embryonic S1  (33) embryonic S3    25 leg disc CME L1  (36) wing disc CME-W2    22 wing disc ML-DmD8    15 wing disc ML-DmD9  (28) wing disc ML-DmD16-c3  (41) wing disc ML-DmD21  (28) wing disc ML-DmD32  (37) haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1  (32) CNS ML-DmBG2-c2  (33) tumorous blood cells mbn2  (34) ovary fGS/OSS  (37) ovary OSC    23 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    26 embryonic 1182-4H    24 embryonic GM2    27 embryonic Kc167    37 embryonic S1    33 embryonic S3    25 leg disc CME L1    36 wing disc CME-W2    22 wing disc ML-DmD8    15 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    28 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    34 ovary fGS/OSS    37 ovary OSC    23 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    26 embryonic 1182-4H    24 embryonic GM2    27 embryonic Kc167    37 embryonic S1    33 embryonic S3    25 leg disc CME L1    36 wing disc CME-W2    22 wing disc ML-DmD8    15 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    28 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    34 ovary fGS/OSS    37 ovary OSC    23 ovary OSS    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    26 embryonic 1182-4H    24 embryonic GM2    27 embryonic Kc167    37 embryonic S1    33 embryonic S3    25 leg disc CME L1    36 wing disc CME-W2    22 wing disc ML-DmD8    15 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    28 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    34 ovary fGS/OSS    37 ovary OSC    23 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (39) Schneider line 2 Sg4  26 embryonic 1182-4H    24 embryonic GM2  27 embryonic Kc167  (37) embryonic S1  (33) embryonic S3    25 leg disc CME L1  (36) wing disc CME-W2    22 wing disc ML-DmD8    15 wing disc ML-DmD9  28 wing disc ML-DmD16-c3  (41) wing disc ML-DmD21  28 wing disc ML-DmD32  (37) haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1  32 CNS ML-DmBG2-c2  (33) tumorous blood cells mbn2  (34) ovary fGS/OSS  (37) ovary OSC    23 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    26 embryonic 1182-4H    24 embryonic GM2    27 embryonic Kc167    37 embryonic S1    33 embryonic S3    25 leg disc CME L1    36 wing disc CME-W2    22 wing disc ML-DmD8    15 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    28 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    34 ovary fGS/OSS    37 ovary OSC    23 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    26 embryonic 1182-4H    24 embryonic GM2    27 embryonic Kc167    37 embryonic S1    33 embryonic S3    25 leg disc CME L1    36 wing disc CME-W2    22 wing disc ML-DmD8    15 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    28 wing disc ML-DmD32    37 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    34 ovary fGS/OSS    37 ovary OSC    23 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    14 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    33 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    14 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult  (33) Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (36) Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (33) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  (28) Paraquat 10 mM 48 hrs, 4-day adult  (28) Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    14 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    33 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    14 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    33 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    14 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    33 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    14 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult  (33) Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (36) Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (33) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  28 Paraquat 10 mM 48 hrs, 4-day adult  28 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    14 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    33 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    14 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    33 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_02 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011708 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4214-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4elav.PLu Syx5GD1743 lethal, with Scer\GAL4Mef2.PR Syx5GD1743 lethal, with Scer\GAL4pnr-MD237 Syx5GD1743 lethal | larval stage, with Scer\GAL4elav.PU Syx5GD1743 lethal | larval stage, with Scer\GAL4GMR.PFa Syx5GD1743 lethal | larval stage, with Scer\GAL4mex1.2.1 Syx5GD1743 lethal | P-stage, with Scer\GAL4elav.PU Syx5GD1743 lethal | P-stage, with Scer\GAL4GMR.PFa Syx5GD1743 lethal | P-stage, with Scer\GAL4mex1.2.1 Syx5GD1743 lethal | recessive Syx5unspecified partially lethal - majority die (with Syx5AR113), with Scer\GAL4[-]: Syx5EP2313 partially lethal - majority die (with Syx5EP2313), with Scer\GAL4[-]: Syx5AR113 Sterility Allele male sterile (with Syx5AR113), with Scer\GAL4[-]: Syx5EP2313 male sterile (with Syx5EP2313), with Scer\GAL4[-]: Syx5AR113 Other Phenotypes Allele chemical resistant | dominant Syx5AR113 cytokinesis defective (with Syx5AR113), with Scer\GAL4[-]: Syx5EP2313 cytokinesis defective (with Syx5EP2313), with Scer\GAL4[-]: Syx5AR113 visible, with Scer\GAL4pnr-MD237 Syx5GD1743 Syx5Scer\UAS.T:Hsap\MYC visible, with Scer\GAL4pnr-MD237, Syx5GD1743 Syx5Scer\UAS.T:Hsap\MYC visible, with Scer\GAL4pnr-MD237, Syx5Scer\UAS.T:Hsap\MYC Syx5GD1743 wild-type, with Scer\GAL4da.G32 Syx5EP2313 Phenotype manifest in Allele adult cuticle, with Scer\GAL4pnr-MD237 Syx5GD1743 adult cuticle, with Scer\GAL4pnr-MD237, Syx5GD1743 Syx5Scer\UAS.T:Hsap\MYC adult cuticle, with Scer\GAL4pnr-MD237, Syx5Scer\UAS.T:Hsap\MYC Syx5GD1743 chaeta, with Scer\GAL4pnr-MD237 Syx5Scer\UAS.T:Hsap\MYC endoplasmic reticulum Syx5dsRNA.cKa Golgi stack Syx5dsRNA.cKa Nebenkern (with Syx5AR113), with Scer\GAL4[-]: Syx5EP2313 Nebenkern (with Syx5EP2313), with Scer\GAL4[-]: Syx5AR113 scutellum, with Scer\GAL4pnr-MD237 Syx5Scer\UAS.T:Hsap\MYC spermatozoon (with Syx5AR113), with Scer\GAL4[-]: Syx5EP2313 spermatozoon (with Syx5EP2313), with Scer\GAL4[-]: Syx5AR113 testis (with Syx5AR113), with Scer\GAL4[-]: Syx5EP2313 testis (with Syx5EP2313), with Scer\GAL4[-]: Syx5AR113 thorax, with Scer\GAL4pnr-MD237 Syx5GD1743
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Syx5 Class Mutagen Stocks Known lesion Syx5AR113 ethyl methanesulfonate 2 Yes Syx5EP2313 P-element activity 2 Yes Syx5EY07901 P-element activity 2 -- Syx5e02643 piggyBac transposase 1 -- Syx5AA73 ethyl methanesulfonate 0 -- Syx5AE48 ethyl methanesulfonate 0 -- Syx5AE73 ethyl methanesulfonate 0 -- Syx5unspecified 0 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Syx5 Class Mutagen Stocks Known lesion Syx5GD1743 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Syx5JF03330 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Syx5dsRNA.cKa in vitro construct - RNAi 0 Yes Syx5KK100952 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Syx5NIG.4214R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Syx5Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Syx5t14.2 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel7065 (Ryder, 2004.4.26) Df(2L)H60-3 (Ashburner et al., 1999, Dawson et al., 1995, Roote, 1996.1.11, Roote, 1996.8) Df(2L)k08106-rv6 (Roote, 1999.7.7) Df(2L)k08106-rv15 (Roote, 1999.7.7) Df(2L)k14608-mr29 (Roote, 2000.6.13) Df(2L)r10 (Ashburner et al., 1990) Df(2L)RA5 (Eberl et al., 1998, Ashburner et al., 1990) Df(2L)RM5 (Ashburner et al., 1990) Df(2L)RN2 (Eberl et al., 1998, Ashburner et al., 1990, Dawson et al., 1995) Df(2L)TE35D-16 (Ashburner et al., 1990) Df(2L)TE35D-21 (Ashburner et al., 1990, Dawson et al., 1995) Df(2L)TE35D-23 (Ashburner et al., 1990)
Not disrupted in	Df(2L)64j (Ashburner et al., 1990) Df(2L)A72 (Ashburner et al., 1990) Df(2L)A267 (Ashburner et al., 1990) Df(2L)ARR1 (Ashburner et al., 1990) Df(2L)b80e4 (Ashburner et al., 1990) Df(2L)cact-255rv64 (Dawson et al., 1995) Df(2L)el20 (Ashburner et al., 1990) Df(2L)el28 (Ashburner et al., 1990) Df(2L)fn12 (Ashburner et al., 1990) Df(2L)fn26 (Ashburner et al., 1990) Df(2L)fn27 (Ashburner et al., 1990) Df(2L)H20 (Ashburner et al., 1990) Df(2L)III18 (Ashburner et al., 1999, Roth et al., 1995) Df(2L)n78l3 (Ashburner et al., 1990) Df(2L)noc11 (Ashburner et al., 1990) Df(2L)osp29 (Eberl et al., 1998) Df(2L)osp38 (Ashburner et al., 1990) Df(2L)rd9 (Ashburner et al., 1990) Df(2L)Sco7 (Ashburner et al., 1990) Df(2L)Scorv16 (Ashburner et al., 1990) Df(2L)Scorv18 (Ashburner et al., 1990) Df(2L)Scorv19 (Ashburner et al., 1990) Df(2L)Scorv25 (Ashburner et al., 1990) Df(2L)SS1 (Ashburner et al., 1990) Df(2L)TE35BC-3 (Ashburner et al., 1990) Df(2L)TE35BC-4 (Ashburner et al., 1990) Df(2L)TE35BC-34 (Ashburner et al., 1990) Df(2L)TE35D-1 (Ashburner et al., 1990) Df(2L)TE35D-2 (Ashburner et al., 1990) Df(2L)TE35D-3 (Ashburner et al., 1990) Df(2L)TE35D-4 (Ashburner et al., 1990) Df(2L)TE35D-6 (Ashburner et al., 1990) Df(2L)TE35D-13 (Eberl et al., 1998, Ashburner et al., 1990, Dawson et al., 1995) Df(2L)TE35D-14 (Eberl et al., 1998, Ashburner et al., 1990) Df(2L)TE35D-15 (Ashburner et al., 1990) Df(2L)TE35D-19 (Ashburner et al., 1990) Df(2L)TE35D-20 (Ashburner et al., 1990) Df(2L)TE35D-22 (Ashburner et al., 1990) In(2L)dpps22Ldppd36R (Ashburner et al., 1990) In(2L)el18 (Eberl et al., 1998, Ashburner et al., 1990, Dawson et al., 1995) In(2LR)Scorv1-DV7 (Ashburner et al., 1990) T(2;3)Scorv7 (Ashburner et al., 1990)
Duplicated in	Dp(2;3)osp3 (Ashburner et al., 1990)
Not duplicated in	Dp(2;1)Scorv23 (Ashburner et al., 1990) Dp(2;2)GYL (Ashburner et al., 1990) Dp(2;2)TE35B-54L (Ashburner et al., 1990) In(2L)C163.41LC158R (Ashburner et al., 1990) In(2L)Scorv17LC158R (Ashburner et al., 1990)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1743} NA P{KK100952} NA P{NIG.4214R} NA P{TRiP.JF03330} NA P{UAS-Syx5.myc} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Syx5EP2313 NA P{EPgy2}Syx5EY07901 NA miscellaneous insertions PBac{RB}Syx5e02643 NA
Gene Ontology: Function, Process & Cellular Component ( 20 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
protein homodimerization activity inferred from physical interaction with Syx5 (assigned by UniProtKB) (Banfield et al., 1994)
Biological Process
CV term Evidence References
cell morphogenesis inferred from mutant phenotype (D'Ambrosio and Vale, 2010) cell proliferation inferred from mutant phenotype (Kondylis et al., 2011) cellular copper ion homeostasis inferred from mutant phenotype (Norgate et al., 2010) cytokinesis inferred from mutant phenotype (Xu et al., 2002, Echard et al., 2004) Golgi organization inferred from mutant phenotype (Kondylis et al., 2011) negative regulation of mitotic cell cycle inferred from mutant phenotype (Kondylis et al., 2011) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) protein secretion inferred from mutant phenotype (D'Ambrosio and Vale, 2010) regulation of cell diameter inferred from mutant phenotype (Kondylis et al., 2011)
Cellular Component
CV term Evidence References
cis-Golgi network inferred from direct assay (assigned by UniProtKB) (Banfield et al., 1994) Golgi apparatus inferred from direct assay (Xu et al., 2002)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
SNAP receptor activity inferred from sequence or structural similarity (Littleton, 2000) inferred from sequence or structural similarity with NCBI_gi:4507293 (McLennan, 2001.5.22)
Biological Process
CV term Evidence References
intracellular protein transport inferred from electronic annotation with InterPro:IPR006012 (FlyBase Curators et al., 2004-) neurotransmitter secretion inferred from sequence or structural similarity with NCBI_gi:4507293 (McLennan, 2001.5.22) non-traceable author statement (Lloyd et al., 2000) synaptic vesicle docking involved in exocytosis inferred from sequence or structural similarity (Littleton, 2000) vesicle-mediated transport inferred from sequence or structural similarity with NCBI_gi:4507293 (McLennan, 2001.5.22) non-traceable author statement (Lloyd et al., 2000)
Cellular Component
CV term Evidence References
endoplasmic reticulum inferred from sequence or structural similarity with UniProtKB:Q08851 (assigned by UniProtKB) (UniProtKB Project Members, 2005.4.12) Golgi membrane inferred from sequence or structural similarity with UniProtKB:Q08851 (assigned by UniProtKB) (UniProtKB Project Members, 2005.4.12) plasma membrane inferred from sequence or structural similarity (Littleton, 2000) inferred from sequence or structural similarity with NCBI_gi:4507293 (McLennan, 2001.5.22)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Syx5 - Bet1 experimental knowledge based (Guruharsha et al., 2011) Syx5 - CG1599 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Syx5 - CG2023 experimental knowledge based (Guruharsha et al., 2011) Syx5 - CG6208 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Gos28 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Nsf2 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Sec22 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Slh anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Syx5 - Snap experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Syx5 - Snap24 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Snap25 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Syb experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Syx5 - Syx13 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Syx16 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Syx18 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Syx1A experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Syx5 - Syx7 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Syx8 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Syx5 - Use1 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Vti1 experimental knowledge based (Guruharsha et al., 2011) Syx5 - Ykt6 experimental knowledge based (Guruharsha et al., 2011) Syx5 - koko experimental knowledge based (Guruharsha et al., 2011) Syx5 - membrin anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Syx5 - usnp experimental knowledge based (Guruharsha et al., 2011) Syx5 - γSnap experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Syx5 allele Gene References Syx5unspecified Nsf2 (Xu et al., 2002)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60977 DroID - A comprehensive database of gene and protein interactions. • FBgn0011708 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34966
Orthologs
OrthoDB Orthologs (37) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG608NV3 Dipteran inclusive ortholog search EOG6VHKGF Insect inclusive ortholog search EOG6XGXW3 Arthropod inclusive ortholog search EOG6JWSWF Metazoa inclusive ortholog search EOG6S262C
Orthologs in Drosophila Species (EOG608NV3)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Syx5     Drosophila simulans   Dsim\GD24051 Y   Drosophila sechellia   Dsec\GM18666 Y   Drosophila erecta   Dere\GG25202 Y   Drosophila yakuba   Dyak\GE21453 Y   Drosophila ananassae   Dana\GF14580 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18038 Y   Drosophila persimilis   Dper\GL16332 Y   Drosophila willistoni   Dwil\GK23986 Y   Drosophila virilis   Dvir\GJ10629 Y   Drosophila mojavensis   Dmoj\GI17075 Y   Drosophila grimshawi   Dgri\GH10314 Y
Orthologs in non-Drosophila Dipterans (EOG6VHKGF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013541   Anopheles gambiae Malaria mosquito  Agam\AGAP008756   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ801941
Orthologs in non-Dipteran Insects (EOG6XGXW3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC552117   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007331   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH23166   Atta cephalotes Leafcutter ant  Acep\ACEP24412   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14547   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15120   Linepithema humile Argentine ant  Lhum\LH15505   Pogonomyrmex barbatus Red harvester ant  Pbar\PB25512   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001052   Bombyx mori Silkmoth  Bmor\BGIBMGA010620   Pediculus humanus Human body louse  Phum\PHUM351020   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07975
Orthologs in non-Insect Arthropods (EOG6JWSWF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\MtnA   Ixodes scapularis Deer tick  Isca\ISCW024059
Orthologs in non-Arthropod Metazoa (EOG6S262C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\syn-3   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014243gn   Danio rerio Zebrafish  Drer\stx5a   Danio rerio Zebrafish  Drer\stx5al   Xenopus tropicalis Western clawed frog  Xtro\stx5a   Mus musculus House mouse  Mmus\Stx5   Rattus norvegicus Norway rat  Rnor\Stx5   Homo sapiens Human  Hsap\STX5
Human Orthologs (1)
Gene OMIM HGNC Hsap\STX5
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24051 Drosophila sechellia Dsec\GM18666 Drosophila erecta Dere\GG25202 Drosophila yakuba Dyak\GE21453 Drosophila ananassae Dana\GF14580 Drosophila pseudoobscura pseudoobscura Dpse\GA18038 Drosophila persimilis Dper\GL16332 Drosophila willistoni Dwil\GK23986 Drosophila virilis Dvir\GJ10629 Drosophila mojavensis Dmoj\GI17075 Drosophila grimshawi Dgri\GH10314
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	17041 w1118; P{EP}Syx5EP2313/CyO 29397 y1 v1; P{TRiP.JF03330}attP2 17413 y1 w67c23; P{EPgy2}Syx5EY07901/CyO 3645 b1 Syx5AR113 pr1 cn1 wxwxt bw1/CyO
Harvard	- PBac{RB}Syx5e02643
Kyoto	107154 b1 Syx5AR113 pr1 cn1 wxwxt bw1/CyO 114446 yd2 w1118 P{ey-FLP.N}2; P{EP}Syx5EP2313 P{neoFRT}40A/CyO y+ 114472 yd2 w1118 P{ey-FLP.N}2; P{EPgy2}Syx5EY07901 P{neoFRT}40A/CyO y+
VDRC	v3859 w1118; P{GD1743}v3859 v3857 w1118; P{GD1743}v3857/TM3
Genomic Clones ( 1 )
	BACR01N08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 120 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD07852
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT09884 GH22093 GM06766 LD02623 LD09049 LD28045 LD29042 LP01930 LP03569 LP05355 LP08421 LP10588 LP21063 RE15171 RE26540 RE30249 RE32789 RE45513 RE58892 RE65153 RE65878 RH40620 RH55940 RH70610 SD03815
Other clones	1767 53067 97745 214449.28 569518 bs48a08 CK01.17A.D4 CK01.62C.C2 Dmel_neb_001066_0354_3644 Dmel_neb_008683_0341_2924 Dmel_neb_013105_0166_1891 EK050441 EK117905 EK123251 EN15048 EP03652 FGM306E11 RE01550 RH01491 RP003014761 RP003048306 RP003053296 RP003055282 RP003061533 RP003088610 RP003089663 RP003092405 RP003104549 RP003106196 RP003116775 RP003120828 RP003122155 RP003125307 RP003131816 RP003143491 RP003157657 RP003160540 RP003167775 RP003175600 RP003184805 RP003185896 RP003196169 RP003196738 RP003215211 RP003245731 RP003247089 RP003257568 RP003267592 RP003270516 RP003278390 RP003295069 RP003305307 RP003308184 RP003326080 RP003328415 RP003338848 RP003350675 RP003350857 RP003359738 RP003359933 RP003366247 RP003375236 RP003381238 RP003397281 RP003397996 RP003407109 RP003413441 RP003428225 RP003451667 RP003455836 RP003462559 RP003467540 RP003485323 RP003497489 RP003510341 RP003515582 RP003516831 RP003516954 RP003521784 RP003533159 RP003540771 RP003540937 RP003543179 RP003547574 RP003549782 RP003556635 RP003561940 RP003568965 RP003583168 RP003589379 RP003590207 RP003613484 RP003657028 RP003659315
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011708 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34966
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Sed5 CG4214 (Zhong, 1999.11) Source for identity of: Syx5 Sed5 (McLennan, 2001.5.22)
Source for database merge of	Source for merge of: Sed5 l(2)35Ff anon-35Fd anon-35Fb (Ashburner et al., 1999)
Additional comments
Other Comments
	S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) dsRNA made from templates generated with primers directed against this gene was tested in an RNAi screen to identify genes that regulate Ca2+ release. (Zhang et al., 2006) Identified in an RNAi screen for host factors that alter infection of SL2 cells by L.monocytogenes. (Agaisse et al., 2005) dsRNA made from templates generated with primers directed against Syx5 that is transfected into S2 treated with Listeria monocytogenes reveals Syx5 to be involved in Listeria monocytogenes entry. (Cheng et al., 2005) dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells. (Echard et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Syx5 is required for the proper function of the Golgi apparatus, and as a consequence required for cytokinesis and spermatid differentiation. (Xu et al., 2002) Transcript identified during molecular analysis of the fzy genomic region. (Dawson et al., 1995) Identified as a transcript in the cni region. (Roth et al., 1995) Studies of Syx5 expressed in COS cells indicates the location of Syx5 is only partially determined by its transmembrane domain and an additional localisation mechanism(s) must exist. (Banfield et al., 1994) 1 allele is discussed but is not named. (Ashburner et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078858 NM_134305 RefSeq (Proteins) • NP_523582 NP_599132 DDBJ / EMBL / GenBank • AA202437 AA803006 AA949231 AA952027 AAF53520 AAL39977 AAN10935 AE003415 AI541895 AW944530 AY069832 CAA55064 CL525967 CZ480421 X78219 Entrez Gene - A searchable database of RefSeq genes. • 34966 UniProtKB/Swiss-Prot • Q24509 UniProtKB/TrEMBL • A4V0S1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Target SNARE coiled-coil domain (IPR000727) Syntaxin, N-terminal (IPR006011) Syntaxin/epimorphin, conserved site (IPR006012) t-SNARE (IPR010989)
Linkouts
	BioGRID - A database of protein and genetic interactions • 60977 DroID - A comprehensive database of gene and protein interactions. • FBgn0011708 DRSC - Results from RNAi screens. • FBgn0011708 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011708 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4214-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011708 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34966 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/syntx5-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34966 modMine - Data generated by the modENCODE project. • FBgn0011708
Synonyms & Secondary IDs ( 29 )
Reported As
Symbol Synonym	anon-35Fb   anon-35Fd   BG:DS02740.12 (Ashburner et al., 1999.10.12, Ashburner et al., 1999) CG4214 (Dunne et al., 2002, Verstreken and Bellen, 2000.9.4, Dorer et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009) DmSyx5 (McLennan, 2001.5.22, Littleton, 2000) dsed5 (Dunne et al., 2002) dSed5 (Banfield et al., 1994) dSed5p (Kondylis and Rabouille, 2003) dSyx5 (Xu et al., 2002, Xu et al., 2002) II (Dawson et al., 1995) l(2)35Ff (Roote, 2000.6.13, Ashburner et al., 1999, Eberl et al., 1998, Roote, 1996.1.11, Spradling et al., 1995, Roote, 1995.5.4, Ashburner et al., 1990, ) Sed5 (Whitfield et al., 2002, Bolshakov et al., 2002, Dunne and Rabouille, 2001, Reese et al., 2000, Reese et al., 2000, Gaasterland et al., 2000, Ashburner et al., 1999, Ashburner et al., 1999, Weimbs et al., 1997, Roth et al., 1995, ) sed5 (Bellen, 2000.12.25, Yogev et al., 2010) SED5 (Lloyd et al., 2000, Lewis, 1994.3.18) SYN5 (Dorer et al., 2006) syn5 (Kondylis et al., 2011) Syntaxin 5 (Edwards et al., 2006) Synx5 (Bard et al., 2006) Syx (Wagh et al., 2006) syx5 (Willingham and Keil, 2004, Ricardo and Lehmann, 2009, Cherry et al., 2006) Syx5 (UniProtKB Project Members, 2005.4.12, Stroschein-Stevenson et al., 2006, Dietzl et al., 2007, Norgate et al., 2010, Rousset et al., 2010, D'Ambrosio and Vale, 2010, Japanese National Institute of Genetics, 2012.5.21, Aikin et al., 2012) Syx5A (Cheng et al., 2005)
Name Synonym	dsyntaxin5 (Schotman et al., 2008) dSyntaxin5 (Schotman et al., 2008) lethal(2)35Ff   syntaxin (Dumstrei et al., 2003) Syntaxin (Kittel et al., 2006, Wagh et al., 2006, Hossain et al., 2008, Pereanu and Hartenstein, 2006) Syntaxin 5 (Edwards et al., 2006, McLennan, 2001.5.22, Zhang et al., 2006, Norgate et al., 2010, Januschke et al., 2007, Rousset et al., 2010, Aikin et al., 2012) syntaxin 5 (Ricardo and Lehmann, 2009) syntaxin5 (Kondylis et al., 2011)
Secondary FlyBase IDs
	FBgn0001996 FBgn0015339 FBgn0020602
References ( 81 )
Generate a list of	All references relating to this gene ( 81 )
List References by type	Research paper  ( 41 ) Supplementary material  ( 9 ) Review  ( 2 ) Personal communication to FlyBase  ( 19 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) Letter  ( 1 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Aikin et al., 2012, PLoS ONE 7(3): e33665 A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila. [FBrf0217808] Kondylis et al., 2011, PLoS ONE 6(2): e17173 Identification of ER Proteins Involved in the Functional Organisation of the Early Secretory Pathway in Drosophila Cells by a Targeted RNAi Screen. [FBrf0213167] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...