BAP45
a component of the Swi/Snf complex (Brahma complex) in Drosophila - suppresses mutations in Polycomb complex genes - a tumor suppressor in imaginal tissues
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Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.49
1.4 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
370 (aa); 43 (kD observed); 43 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Snr1 using the Feature Mapper tool.
Comment: maternally deposited
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Nothern blotting with Snr1 specific riboprobes confirmed that the transcript can be detected at all stages of development. A transient increase in message was observed late in third instar larvae and in pupae corresponding to the time of rising titers of ecdysone hormone. In contrast to previous experiments expression was detected in adult males.
Snr1 transcripts are expressed maximally in unfertilized eggs and early embryos, indicating maternal contributions. Levels drop steadily during embryogenesis with a dramatic drop at 16hrs. Low levels of Snr1 transcripts are observed in larvae, pupae, and adult females. The temporal pattern of Snr1 transcription is very similar to that of brm.
Fluorescent confocal imaging of Snr1 localization in similarly staged embryos showed that the protein was tightly associated with chromatin in the vast majority of nuclei. Occasionally, Snr1 appeared more diffuse in the cytosol and closer examination revealed that the majority of chromosomes were in metaphase.
In the larval CNS expression of Snr1 protein is restricted to a subset of cells in the optic lobe. The protein is also restricted to subsets of cells in the leg and wing discs with low or undetectable expression in some discs. The Snr1 protein is detected at relatively high levels in developing eye discs both anterior and posterior to the morphogenetic furrow. The protein is detected in a punctate pattern within the nuclei of salivary gland cells. Staining is also observed in germarium regions 2 and 3 in the ovariole. A few cells of the somatic follicular sheath and nurse cell nuclei and the developing oocyte also express low levels of the Snr1 protein.
Snr1 protein is detected at its highest level in early embryos. Low levels are found in larvae, pupae, and adult females. Snr1 protein is found in all nuclei of the embryo through germ band extension. After germ band retraction it is located almost exclusively in the brain and CNS. Snr1 protein is detected in salivary glands and imaginal discs in larvae
GBrowse - Visual display of RNA-Seq signals
View Dmel\Snr1 in GBrowse 23-47.5
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Snr1 CG1064
dsRNA has been made from templates generated with primers directed against this gene. RNAi of Snr1 results in dorsal overextension of primary dendrites and a reduction in lateral branching. RNAi also causes defects in muscle, defects in the epidermis, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
Loss of Snr1 function results in ectopic vein material in the wing.
The Snr1 and brm proteins are present in a large complex and co-precipitate from extracts, these results suggest that the Drosophila counterpart of the yeast SWI/SNF complex plays an important role in counteracting the repressive effects of chromatin on homeotic gene transcription during development.
Snr1 is essential for viability. Phenotypic studies and genetic interactions suggest that Snr1 and brm act together, and with trx, to regulate homeotic gene transcription. Snr1 and brm proteins are present in a large complex, this complex may play an important role in maintaining homeotic gene transcription during development by counteracting the repressive effect of chromatin.