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Gene Dmel\wee

General Information
Symbol	Dmel\wee	Species	D. melanogaster
Name	wee	Annotation symbol	CG4488
Feature type	protein_coding_gene	FlyBase ID	FBgn0011737
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	dwee1, Wee1, Dwee
Genomic Location
Chromosome (arm)	2L	Recombination map	2-
Cytogenetic map	27C4-27C4	Sequence location	2L:6,911,090..6,914,138 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene wee is referred to in FlyBase by the symbol Dmel\wee (CG4488, FBgn0011737). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein tyrosine kinase activity. There is experimental evidence that it is involved in the biological process: mitotic cell cycle, embryonic; centrosome localization; centrosome separation; negative regulation of cyclin-dependent protein serine/threonine kinase activity; mitotic cell cycle checkpoint; nuclear division; spindle assembly; cell cycle. 17 alleles are reported. The phenotypes of these alleles are annotated with: wing margin; interommatidial bristle; mitotic cell cycle; adult thorax; wing; ommatidium; scutellum. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Wee1-like protein kinase. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult brain, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, larval fat body, larval salivary gland, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1633731_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of wee. Gene sequence location is 2L:6911090..6914138.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 27C4-27C4   Limits computationally determined from genome sequence between P{PZ}Rca103300&P{lacW}l(2)k09022k09022 and P{lacW}xl6k00230&P{EP}Hrb27CEP748
Experimentally Determined Cytological Location
Cytogenetic map Notes References 27C-27C     (Campbell, 1994.11.16) 27C-27C   (determined by in situ hybridisation)   (Campbell et al., 1995)
Experimentally Determined Recombination Data
Location	2- (Campbell, 1994.11.16)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\wee for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Gene model reviewed during 5.46 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) wee-RA FBtr0079371  NM_057687   2399   609 wee-RB FBtr0332329  NM_001273238   2652   609
Additional Transcript Data & Comments
Reported size (kB)	2.2 (compiled cDNA) (Campbell et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein wee-PA   FBpp0078999   68.8   609   7.51     NP_477035   AAF52453 wee-PB   FBpp0304607   68.8   609   7.51     NP_001260167   AGB92703
Additional Polypeptide Data & Comments
Reported size (kDa)	618 (aa); 69 (kD predicted) (Campbell et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Protein kinase-like domain (IPR011009) Wee1-like protein kinase (IPR017164) Protein kinase, ATP binding site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201463   AA201844   AA263411   AA392713   AA536469   AA941432   AA941454   AA948877   AA950517   AA979116   AA979120   AE014134 AAF52453   AC004277   AC007977   AE014134 AGB92703   AI106909   AI113887   AI511806   AI513186   AI516727   AI519722   AI519747   AI519750   AY118942 AAM50802   BF494621   BF502671   BF504799   BF506650   BG634563   BG638707   BI166283   BI168642   BI169950   BI229147   BI234815   BI240212   BI363336   BI364197   BI364917   BI365782   BI366271   BI372113   BI376167   BI565868   BI568754   BI577106   BI581133   BI585324   BI585325   BI589485   BI589492   BI628100   CO178809   CO189739   CO264637   CO274810   CO278613   CO285084   CO312675   CO332721   CO333723   EC051540   EC051541   EC056098   EC072679   EC073170   EC086827   EC087319   EC090669   EC198194   EC209799   EC209955   EC211196   EC225485   EC229862   EC230256   EC237585   EC240630   GH756622   GH760406   GH776841   GH787193   GH796436   GH802927   GH807938   GH809808   GH813643   GH817778   GH817920   GH825325   GH830827   GH839499   GH845861   GH854883   GH860627   GH862238   GH863933   GH865829   GH874292   GH878745   GH884488   GH885229   GH897621   GH900491   GH901274   GH903224   GH912816   GH920519   GH920972   U17223 AAC46913
UniProtKB/Swiss-Prot	P54350
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   pole plasm   (Fisher et al., 2012) Comment:maternally deposited organism   • pole plasm (Fisher et al., 2012) Comment:maternally deposited embryonic stage 1 -- 5   organism | ubiquitous   (Campbell et al., 1995) embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 6   pole cell   (Campbell et al., 1995) embryonic stage 7   mesoderm anlage   (Campbell et al., 1995) embryonic stage 7 -- 8   pole cell   (Fisher et al., 2012) embryonic stage 14   embryonic/larval foregut   (Campbell et al., 1995) embryonic/larval midgut   (Campbell et al., 1995) embryonic/larval hindgut   (Campbell et al., 1995) embryonic stage 14 -- 17   presumptive embryonic/larval central nervous system   (Campbell et al., 1995) first instar larval stage   embryonic/larval brain   (Campbell et al., 1995)
Additional Descriptive Data
	Early maternal wee expression is ubiquitous, and declines by gastrulation, with strong staining still detected in the pole cells. At stage 7, zygotic expression is detected in the presumptive mesoderm. After germ band retraction, wee expression is detected in the gut and the CNS. CNS expression continues to be detected in late embryos and in first larval instar. (Campbell et al., 1995)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Campbell et al., 1995)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult brain, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, larval fat body, larval salivary gland, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    312.65 Larval Midgut    41.2 Larval Hindgut    69.4 Larval Malpighian Tubules    49.9 Larval Fat Body    179.5 Larval Salivary Gland    130.2 Larval Trachea    71.3 Larval Carcass    63.925 Adult Head    166.1 Adult Eye    79.65 Adult Brain    548.9 Adult Thoracic-Abdominal Ganglion    487.1 Adult Crop    69.4 Adult Midgut    35.5 Adult Hindgut    51 Adult Malpighian Tubules    43.9 Adult Fat Body    53.8 Adult Salivary Gland    60.3 Adult Heart    40.8 Adult VirginFemale Spermatheca    66.3 Adult InseminatedFemale Spermatheca    66.2 Adult Ovary    450.9 Adult Testis    659.3 Adult Male Accessory Gland    84.6 Adult Carcass    42.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    312.65 Larval Midgut    41.2 Larval Hindgut    69.4 Larval Malpighian Tubules    49.9 Larval Fat Body    179.5 Larval Salivary Gland    130.2 Larval Trachea    71.3 Larval Carcass    63.925 Adult Head    166.1 Adult Eye    79.65 Adult Brain  (548.9) Adult Thoracic-Abdominal Ganglion    487.1 Adult Crop    69.4 Adult Midgut    35.5 Adult Hindgut    51 Adult Malpighian Tubules    43.9 Adult Fat Body    53.8 Adult Salivary Gland    60.3 Adult Heart    40.8 Adult VirginFemale Spermatheca    66.3 Adult InseminatedFemale Spermatheca    66.2 Adult Ovary    450.9 Adult Testis  (659.3) Adult Male Accessory Gland    84.6 Adult Carcass    42.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    312.65 Larval Midgut    41.2 Larval Hindgut    69.4 Larval Malpighian Tubules    49.9 Larval Fat Body    179.5 Larval Salivary Gland    130.2 Larval Trachea    71.3 Larval Carcass    63.925 Adult Head    166.1 Adult Eye    79.65 Adult Brain    548.9 Adult Thoracic-Abdominal Ganglion    487.1 Adult Crop    69.4 Adult Midgut    35.5 Adult Hindgut    51 Adult Malpighian Tubules    43.9 Adult Fat Body    53.8 Adult Salivary Gland    60.3 Adult Heart    40.8 Adult VirginFemale Spermatheca    66.3 Adult InseminatedFemale Spermatheca    66.2 Adult Ovary    450.9 Adult Testis    659.3 Adult Male Accessory Gland    84.6 Adult Carcass    42.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    312.65 Larval Midgut    41.2 Larval Hindgut    69.4 Larval Malpighian Tubules    49.9 Larval Fat Body    179.5 Larval Salivary Gland    130.2 Larval Trachea    71.3 Larval Carcass    63.925 Adult Head    166.1 Adult Eye    79.65 Adult Brain    548.9 Adult Thoracic-Abdominal Ganglion    487.1 Adult Crop    69.4 Adult Midgut    35.5 Adult Hindgut    51 Adult Malpighian Tubules    43.9 Adult Fat Body    53.8 Adult Salivary Gland    60.3 Adult Heart    40.8 Adult VirginFemale Spermatheca    66.3 Adult InseminatedFemale Spermatheca    66.2 Adult Ovary    450.9 Adult Testis    659.3 Adult Male Accessory Gland    84.6 Adult Carcass    42.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    312.65 Larval Midgut    41.2 Larval Hindgut    69.4 Larval Malpighian Tubules    49.9 Larval Fat Body    179.5 Larval Salivary Gland    130.2 Larval Trachea    71.3 Larval Carcass    63.925 Adult Head    166.1 Adult Eye    79.65 Adult Brain    548.9 Adult Thoracic-Abdominal Ganglion    487.1 Adult Crop    69.4 Adult Midgut    35.5 Adult Hindgut    51 Adult Malpighian Tubules    43.9 Adult Fat Body    53.8 Adult Salivary Gland    60.3 Adult Heart    40.8 Adult VirginFemale Spermatheca    66.3 Adult InseminatedFemale Spermatheca    66.2 Adult Ovary    450.9 Adult Testis    659.3 Adult Male Accessory Gland    84.6 Adult Carcass    42.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    312.65 Larval Midgut    41.2 Larval Hindgut    69.4 Larval Malpighian Tubules    49.9 Larval Fat Body    179.5 Larval Salivary Gland    130.2 Larval Trachea    71.3 Larval Carcass    63.925 Adult Head    166.1 Adult Eye    79.65 Adult Brain  548.9 Adult Thoracic-Abdominal Ganglion    487.1 Adult Crop    69.4 Adult Midgut    35.5 Adult Hindgut    51 Adult Malpighian Tubules    43.9 Adult Fat Body    53.8 Adult Salivary Gland    60.3 Adult Heart    40.8 Adult VirginFemale Spermatheca    66.3 Adult InseminatedFemale Spermatheca    66.2 Adult Ovary    450.9 Adult Testis  659.3 Adult Male Accessory Gland    84.6 Adult Carcass    42.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    312.65 Larval Midgut    41.2 Larval Hindgut    69.4 Larval Malpighian Tubules    49.9 Larval Fat Body    179.5 Larval Salivary Gland    130.2 Larval Trachea    71.3 Larval Carcass    63.925 Adult Head    166.1 Adult Eye    79.65 Adult Brain    548.9 Adult Thoracic-Abdominal Ganglion    487.1 Adult Crop    69.4 Adult Midgut    35.5 Adult Hindgut    51 Adult Malpighian Tubules    43.9 Adult Fat Body    53.8 Adult Salivary Gland    60.3 Adult Heart    40.8 Adult VirginFemale Spermatheca    66.3 Adult InseminatedFemale Spermatheca    66.2 Adult Ovary    450.9 Adult Testis    659.3 Adult Male Accessory Gland    84.6 Adult Carcass    42.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    312.65 Larval Midgut    41.2 Larval Hindgut    69.4 Larval Malpighian Tubules    49.9 Larval Fat Body    179.5 Larval Salivary Gland    130.2 Larval Trachea    71.3 Larval Carcass    63.925 Adult Head    166.1 Adult Eye    79.65 Adult Brain    548.9 Adult Thoracic-Abdominal Ganglion    487.1 Adult Crop    69.4 Adult Midgut    35.5 Adult Hindgut    51 Adult Malpighian Tubules    43.9 Adult Fat Body    53.8 Adult Salivary Gland    60.3 Adult Heart    40.8 Adult VirginFemale Spermatheca    66.3 Adult InseminatedFemale Spermatheca    66.2 Adult Ovary    450.9 Adult Testis    659.3 Adult Male Accessory Gland    84.6 Adult Carcass    42.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    166.1   NA  Eye    79.65   NA  Brain    548.9   312.65  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    487.1   NA  Crop    69.4   41.2  Midgut    35.5   69.4  Hindgut    51   49.9  Malpighian Tubules    43.9   179.5  Fat Body    53.8   130.2  Salivary Gland    60.3   NA  Heart    40.8   71.3  Trachea    NA   NA  VirginFemale Spermatheca    66.3   NA  InseminatedFemale Spermatheca    66.2   NA  Ovary    450.9   NA  Testis    659.3   NA  Male Accessory Gland    84.6   63.925  Carcass    42.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    11 central nervous system, pupae P8    20 head, virgin 1-day female    6 head, virgin 4-day female    9 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    8 head, mated 20-day female    8 head, mated 1-day male    12 head, mated 4-day male    15 head, mated 20-day male    19 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    15 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    29 ovary, mated 4-day female    21 testis, mated 4-day male    49 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (34) central nervous system, larvae L3    11 central nervous system, pupae P8    20 head, virgin 1-day female    6 head, virgin 4-day female    9 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    8 head, mated 20-day female    8 head, mated 1-day male    12 head, mated 4-day male    15 head, mated 20-day male    19 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    15 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female  (29) ovary, mated 4-day female    21 testis, mated 4-day male  (49) accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    11 central nervous system, pupae P8    20 head, virgin 1-day female    6 head, virgin 4-day female    9 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    8 head, mated 20-day female    8 head, mated 1-day male    12 head, mated 4-day male    15 head, mated 20-day male    19 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    15 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    29 ovary, mated 4-day female    21 testis, mated 4-day male    49 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    11 central nervous system, pupae P8    20 head, virgin 1-day female    6 head, virgin 4-day female    9 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    8 head, mated 20-day female    8 head, mated 1-day male    12 head, mated 4-day male    15 head, mated 20-day male    19 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    15 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    29 ovary, mated 4-day female    21 testis, mated 4-day male    49 accessory gland, mated 4-day male    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    11 central nervous system, pupae P8    20 head, virgin 1-day female    6 head, virgin 4-day female    9 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    8 head, mated 20-day female    8 head, mated 1-day male    12 head, mated 4-day male    15 head, mated 20-day male    19 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    15 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    29 ovary, mated 4-day female    21 testis, mated 4-day male    49 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (34) central nervous system, larvae L3    11 central nervous system, pupae P8    20 head, virgin 1-day female    6 head, virgin 4-day female    9 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    8 head, mated 20-day female    8 head, mated 1-day male    12 head, mated 4-day male    15 head, mated 20-day male    19 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    15 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female  29 ovary, mated 4-day female    21 testis, mated 4-day male  (49) accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    11 central nervous system, pupae P8    20 head, virgin 1-day female    6 head, virgin 4-day female    9 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    8 head, mated 20-day female    8 head, mated 1-day male    12 head, mated 4-day male    15 head, mated 20-day male    19 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    15 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    29 ovary, mated 4-day female    21 testis, mated 4-day male    49 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    11 central nervous system, pupae P8    20 head, virgin 1-day female    6 head, virgin 4-day female    9 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    8 head, mated 20-day female    8 head, mated 1-day male    12 head, mated 4-day male    15 head, mated 20-day male    19 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    1 fat body, white prepupae    8 fat body, pupae P8    15 carcass, larvae L3 wandering    6 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    29 ovary, mated 4-day female    21 testis, mated 4-day male    49 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    32 embryo 08-10hr    33 embryo 10-12hr    22 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    18 embryo 18-20hr    11 embryo 20-22hr    24 embryo 22-24hr    21 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    16 white prepupae new    15 white prepupae 12hr    26 white prepupae 24hr    34 pupae 2d postWPP    32 pupae 3d postWPP    24 pupae 4d postWPP    20 adult male 01day    29 adult male 05day    43 adult male 30day    43 adult female 01day    18 adult female 05day    31 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (62) embryo 02-04hr  (55) embryo 04-06hr  (35) embryo 06-08hr  (32) embryo 08-10hr  (33) embryo 10-12hr    22 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    18 embryo 18-20hr    11 embryo 20-22hr    24 embryo 22-24hr    21 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    16 white prepupae new    15 white prepupae 12hr  26 white prepupae 24hr  (34) pupae 2d postWPP  (32) pupae 3d postWPP    24 pupae 4d postWPP    20 adult male 01day  (29) adult male 05day  (43) adult male 30day  (43) adult female 01day    18 adult female 05day  (31) adult female 30day  (32) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    32 embryo 08-10hr    33 embryo 10-12hr    22 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    18 embryo 18-20hr    11 embryo 20-22hr    24 embryo 22-24hr    21 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    16 white prepupae new    15 white prepupae 12hr    26 white prepupae 24hr    34 pupae 2d postWPP    32 pupae 3d postWPP    24 pupae 4d postWPP    20 adult male 01day    29 adult male 05day    43 adult male 30day    43 adult female 01day    18 adult female 05day    31 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    32 embryo 08-10hr    33 embryo 10-12hr    22 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    18 embryo 18-20hr    11 embryo 20-22hr    24 embryo 22-24hr    21 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    16 white prepupae new    15 white prepupae 12hr    26 white prepupae 24hr    34 pupae 2d postWPP    32 pupae 3d postWPP    24 pupae 4d postWPP    20 adult male 01day    29 adult male 05day    43 adult male 30day    43 adult female 01day    18 adult female 05day    31 adult female 30day    32 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    32 embryo 08-10hr    33 embryo 10-12hr    22 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    18 embryo 18-20hr    11 embryo 20-22hr    24 embryo 22-24hr    21 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    16 white prepupae new    15 white prepupae 12hr    26 white prepupae 24hr    34 pupae 2d postWPP    32 pupae 3d postWPP    24 pupae 4d postWPP    20 adult male 01day    29 adult male 05day    43 adult male 30day    43 adult female 01day    18 adult female 05day    31 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (62) embryo 02-04hr  (55) embryo 04-06hr  (35) embryo 06-08hr  32 embryo 08-10hr  (33) embryo 10-12hr    22 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    18 embryo 18-20hr    11 embryo 20-22hr    24 embryo 22-24hr    21 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    16 white prepupae new    15 white prepupae 12hr  26 white prepupae 24hr  (34) pupae 2d postWPP  32 pupae 3d postWPP    24 pupae 4d postWPP    20 adult male 01day  29 adult male 05day  (43) adult male 30day  (43) adult female 01day    18 adult female 05day  31 adult female 30day  32 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    32 embryo 08-10hr    33 embryo 10-12hr    22 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    18 embryo 18-20hr    11 embryo 20-22hr    24 embryo 22-24hr    21 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    16 white prepupae new    15 white prepupae 12hr    26 white prepupae 24hr    34 pupae 2d postWPP    32 pupae 3d postWPP    24 pupae 4d postWPP    20 adult male 01day    29 adult male 05day    43 adult male 30day    43 adult female 01day    18 adult female 05day    31 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    32 embryo 08-10hr    33 embryo 10-12hr    22 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    18 embryo 18-20hr    11 embryo 20-22hr    24 embryo 22-24hr    21 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    16 white prepupae new    15 white prepupae 12hr    26 white prepupae 24hr    34 pupae 2d postWPP    32 pupae 3d postWPP    24 pupae 4d postWPP    20 adult male 01day    29 adult male 05day    43 adult male 30day    43 adult female 01day    18 adult female 05day    31 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    11 embryonic 1182-4H    12 embryonic GM2    7 embryonic Kc167    10 embryonic S1    22 embryonic S3    12 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    9 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    15 ovary fGS/OSS    18 ovary OSC    11 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    11 embryonic 1182-4H    12 embryonic GM2    7 embryonic Kc167    10 embryonic S1    22 embryonic S3    12 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    9 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    15 ovary fGS/OSS    18 ovary OSC    11 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    11 embryonic 1182-4H    12 embryonic GM2    7 embryonic Kc167    10 embryonic S1    22 embryonic S3    12 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    9 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    15 ovary fGS/OSS    18 ovary OSC    11 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    11 embryonic 1182-4H    12 embryonic GM2    7 embryonic Kc167    10 embryonic S1    22 embryonic S3    12 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    9 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    15 ovary fGS/OSS    18 ovary OSC    11 ovary OSS    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    11 embryonic 1182-4H    12 embryonic GM2    7 embryonic Kc167    10 embryonic S1    22 embryonic S3    12 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    9 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    15 ovary fGS/OSS    18 ovary OSC    11 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    11 embryonic 1182-4H    12 embryonic GM2    7 embryonic Kc167    10 embryonic S1    22 embryonic S3    12 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    9 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    15 ovary fGS/OSS    18 ovary OSC    11 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    11 embryonic 1182-4H    12 embryonic GM2    7 embryonic Kc167    10 embryonic S1    22 embryonic S3    12 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    9 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    15 ovary fGS/OSS    18 ovary OSC    11 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    11 embryonic 1182-4H    12 embryonic GM2    7 embryonic Kc167    10 embryonic S1    22 embryonic S3    12 leg disc CME L1    13 wing disc CME-W2    11 wing disc ML-DmD8    9 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    11 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    13 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    15 ovary fGS/OSS    18 ovary OSC    11 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_21 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • LD27552 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011737 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4488-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for wee Stages(s) 1-3 Stages(s) 4-6 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | maternal effect weeES1 lethal | embryonic stage | maternal effect | recessive weeDS1 lethal | embryonic stage | non-rescuable maternal effect | recessive weeES1 viable weeunspecified viable, with Scer\GAL4pnr-MD237 weeGD11328 Sterility Allele female sterile | recessive weeDS1 weeES1 weeES2 male fertile weeES1 Other Phenotypes Allele chemical sensitive (with Df(2L)Dwee1-W05) weeES1 mitotic cell cycle defective weeES1 mitotic cell cycle defective, with Scer\GAL4unspecified weeScer\UAS.cPa mitotic cell cycle defective | maternal effect weeES1 visible weeGMR.PP visible, with Scer\GAL4ap-md544 weeScer\UAS.cPa visible, with Scer\GAL4GMR.PF weeScer\UAS.cPa visible, with Scer\GAL4pnr-MD237 weeScer\UAS.cPa visible, with Scer\GAL4sd-SG29.1 weeScer\UAS.cPa wild-type wee+tPa Phenotype manifest in Allele adult thorax, with Scer\GAL4pnr-MD237 weeScer\UAS.cPa anaphase & centrosome & cycle 12 embryo | maternal effect weeES1 anaphase & centrosome & cycle 13 embryo | maternal effect weeES1 anaphase & condensed nuclear chromosome & cycle 13 embryo | maternal effect weeES1 aster | embryonic cycle 12 | maternal effect weeES1 aster | embryonic cycle 13 | maternal effect weeES1 chaeta & adult thorax | dorsal, with Scer\GAL4ap-md544 weeScer\UAS.cPa chaeta & adult thorax | dorsal, with Scer\GAL4pnr-MD237 weeScer\UAS.cPa interommatidial bristle, with Scer\GAL4GMR.PF weeScer\UAS.cPa interphase & centrosome & cycle 12 embryo | maternal effect weeES1 interphase of mitotic cell cycle | maternal effect weeES1 metaphase & centrosome & cycle 12 embryo | maternal effect weeES1 microtubule organizing center | embryonic cycle 12 | maternal effect weeES1 microtubule organizing center | embryonic cycle 13 | maternal effect weeES1 mitotic anaphase | embryonic cycle 12 | maternal effect weeES1 mitotic cell cycle weeES1 mitotic cell cycle, with Scer\GAL4unspecified weeScer\UAS.cPa mitotic telophase | embryonic cycle 12 | maternal effect weeES1 nucleus | ectopic | embryonic stage 4 | maternal effect weeES1 nucleus | embryonic cycle 11 weeES1 nucleus | embryonic cycle 12 weeES1 ommatidium weeGMR.PP ommatidium, with Scer\GAL4GMR.PF weeScer\UAS.cPa scutellum, with Scer\GAL4ap-md544 weeScer\UAS.cPa spindle | embryonic cycle 12 | maternal effect weeES1 spindle | embryonic cycle 13 | maternal effect weeES1 wing, with Scer\GAL4ap-md544 weeScer\UAS.cPa wing margin, with Scer\GAL4sd-SG29.1 weeScer\UAS.cPa
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of wee Class Mutagen Stocks Known lesion weeES1 amorphic allele - genetic evidence ethyl methanesulfonate 2 Yes weeDS1 hypomorphic allele - genetic evidence diepoxybutane 2 Yes weeG3116 P-element activity 1 -- weeES2 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes weeunspecified 0 -- weeZII-2186 0 --
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of wee Class Mutagen Stocks Known lesion weeGD11328 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes weeGL00039 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes weeKK109180 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes wee+tPa in vitro construct - genomic fragment 0 Yes wee9A.Scer\UAS.T:Avic\GFP-CFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes weeGMR.PP in vitro construct - regulatory fusion 0 Yes weehs.PP in vitro construct - regulatory fusion 0 Yes weehs.T:Ivir\HA1 in vitro construct - coding region fusion 0 Yes weeScer\UAS.cPa in vitro construct - regulatory fusion 0 Yes weeScer\UAS.P\T.cPa in vitro construct - regulatory fusion 0 Yes weeScer\UAS.T:Avic\GFP-CFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)BSC108 (Deal and Cook, 2005.7.11)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11328} NA P{KK109180} NA P{TRiP.GL00039} NA P{UASp-wee.P} NA P{UAS-wee.9A.CFP} NA P{UAS-wee.CFP} NA P{UAS-wee.P} NA heat-shock construct P{hs-wee.HA} NA P{hs-wee.P} NA characterization construct P{GMR-wee.P} NA P{wee+tPa} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}weeG3116 NA
Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )
Terms Based on Experimental Evidence ( 11 terms )
Molecular Function
CV term Evidence References
protein tyrosine kinase activity inferred from direct assay (Campbell et al., 1995, Garcia et al., 2009)
Biological Process
CV term Evidence References
cell cycle inferred from mutant phenotype (Kiger et al., 2003) centrosome localization inferred from mutant phenotype (Stumpff et al., 2005) centrosome separation inferred from mutant phenotype (Stumpff et al., 2005) mitotic cell cycle, embryonic inferred from mutant phenotype (Price et al., 1999) mitotic cell cycle checkpoint inferred from direct assay (Campbell et al., 1995) negative regulation of cyclin-dependent protein serine/threonine kinase activity inferred from direct assay (Campbell et al., 1995) inferred from mutant phenotype (Stumpff et al., 2004) nuclear division inferred from mutant phenotype (Price et al., 1999) spindle assembly inferred from mutant phenotype (Stumpff et al., 2005)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Campbell et al., 1995)
Terms Based on Predictions or Assertions ( 10 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441 (FlyBase Curators et al., 2004-) magnesium ion binding inferred from electronic annotation with InterPro:IPR017164 (FlyBase Curators et al., 2004-) non-membrane spanning protein tyrosine kinase activity inferred from electronic annotation with InterPro:IPR017164 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein tyrosine kinase activity non-traceable author statement (Swiss-Prot Project Members, 1996.10.1)
Biological Process
CV term Evidence References
embryonic development via the syncytial blastoderm traceable author statement (Lee and Orr-Weaver, 2003) mitosis inferred from electronic annotation with InterPro:IPR017164 (FlyBase Curators et al., 2004-) protein phosphorylation non-traceable author statement (Morrison et al., 2000) regulation of mitosis non-traceable author statement (Swiss-Prot Project Members, 1996.10.1)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1996.10.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
wee - 128up anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - AIF anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Aats-glupro anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Aats-lys anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Arc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Arf79F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - BCAS2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG11505 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG12264 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG13472 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG15784 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG1785 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG2199 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG31694 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG32479 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG3262 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG3689 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG4849 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG7920 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CG8043 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CaBP1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CkIIα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Cp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - CycB anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Dgp-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - DnaJ-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Dp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Eb1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Ect4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - EfTuM anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - ImpL3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Int6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Khc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - LRP1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - LanB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Map205 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Ns1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Prp8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Psi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Ranbp9 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - RpS10b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Srp72 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Top3β anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - Upf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - aub anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - eIF-2β anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - eIF-4B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - hts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - hyd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - kst anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - l(2)35Df anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - l(3)72Ab anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - me31B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - nonA-l anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - pav anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - polo anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - pont anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - ppan anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - pyd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - pzg anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - qkr54B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - su(f) anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - sub anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - tws anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wee - vig anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions wee allele Gene References wee+tPa mei-41 (Price et al., 2000) weeDS1 mei-41 (Price et al., 2000) weeES1 lok (Stumpff et al., 2004) weeGMR.PP cdc2 (Price et al., 2002) p53 (Price et al., 2002) weeScer\UAS.cPa Dp (Reis and Edgar, 2004) stg (Fichelson and Gho, 2004) trbl (Price et al., 2002) unspecified lok (Stumpff et al., 2005) Pten (Kiger et al., 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60116 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011737 DroID - A comprehensive database of gene and protein interactions. • FBgn0011737 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33965 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P54350
Orthologs
OrthoDB Orthologs (48) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6NS3X4 Dipteran inclusive ortholog search EOG6P8FSB Insect inclusive ortholog search EOG63N67S Arthropod inclusive ortholog search EOG6MW6N6 Metazoa inclusive ortholog search EOG68SVM8
Orthologs in Drosophila Species (EOG6NS3X4)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\wee     Drosophila simulans   Dsim\GD22535 Y   Drosophila sechellia   Dsec\GM13760 Y   Drosophila erecta   Dere\GG23574 Y   Drosophila yakuba   Dyak\GE18396 Y   Drosophila ananassae   Dana\GF14473 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18218 Y   Drosophila persimilis   Dper\GL25606 Y   Drosophila willistoni   Dwil\GK24780 Y   Drosophila virilis   Dvir\GJ17570 Y   Drosophila mojavensis   Dmoj\GI17745 Y   Drosophila grimshawi   Dgri\GH13165 Y
Orthologs in non-Drosophila Dipterans (EOG6P8FSB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011376   Anopheles gambiae Malaria mosquito  Agam\AGAP007798   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ016939
Orthologs in non-Dipteran Insects (EOG63N67S)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\wee   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG000593   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG000594   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH21274   Atta cephalotes Leafcutter ant  Acep\ACEP19469   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16550   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20898   Linepithema humile Argentine ant  Lhum\LH17119   Pogonomyrmex barbatus Red harvester ant  Pbar\PB22362   Solenopsis invicta Red fire ant  Sinv\SINV14654   Acyrthosiphon pisum Pea aphid  Apim\ACYPI009690   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000952   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002384   Bombyx mori Silkmoth  Bmor\BGIBMGA011250   Pediculus humanus Human body louse  Phum\PHUM378600   Tribolium castaneum Red flour beetle  Tcas\GLEAN_15825-OG5550
Orthologs in non-Insect Arthropods (EOG6MW6N6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_309101   Ixodes scapularis Deer tick  Isca\ISCW009524
Orthologs in non-Arthropod Metazoa (EOG68SVM8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009093gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027012gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014185gn   Danio rerio Zebrafish  Drer\wee1   Danio rerio Zebrafish  Drer\ENSDARG00000012718   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031988   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000021169   Gallus gallus Domestic chicken  Ggal\WEE2   Gallus gallus Domestic chicken  Ggal\WEE1   Mus musculus House mouse  Mmus\Wee2   Mus musculus House mouse  Mmus\Wee1   Rattus norvegicus Norway rat  Rnor\Wee2   Rattus norvegicus Norway rat  Rnor\Wee1   Homo sapiens Human  Hsap\WEE1   Homo sapiens Human  Hsap\WEE2
Human Orthologs (2)
Gene OMIM HGNC Hsap\WEE1     Hsap\WEE2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD22535 Drosophila sechellia Dsec\GM13760 Drosophila erecta Dere\GG23574 Drosophila yakuba Dyak\GE18396 Drosophila ananassae Dana\GF14473 Drosophila pseudoobscura pseudoobscura Dpse\GA18218 Drosophila persimilis Dper\GL25606 Drosophila willistoni Dwil\GK24780 Drosophila virilis Dvir\GJ17570 Drosophila mojavensis Dmoj\GI17745 Drosophila grimshawi Dgri\GH13165
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	27096 y1 w*; P{EP}weeG3116 35170 y1 sc* v1; P{TRiP.GL00039}attP2 3499 w1; weeDS1 cn1/CyO, P{ActGFP}JMR1 5833 w1; weeES1 cn1/CyO, P{ActGFP}JMR1
Kyoto	107070 w1; weeDS1 cn1/CyO, P{ActGFP}JMR1 108455 w1; weeES1 cn1/CyO, P{ActGFP}JMR1
VDRC	v106329 P{KK109180}VIE-260B v26543 w1118; P{GD11328}v26543/TM3
Genomic Clones ( 2 )
	BACR13J07 BACR20B21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 91 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD27552
Other clones	DPiM_LD27552
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT03319 AT06828 AT09184 AT18468 AT30807 GH06166 GH10382 LD03790 LD04481 LD07263 LD11818 LD17087 LD25568 LD25604 LD30344 LD33387 LD39423 LD39493 LD39503 LD42736 LD43333 LD45388 RE06357 RE09470 RE11270 RE26866 RE30585 RE36910 RE48118 RE49261 RE50118 RE51161 RE51772 RE58920 RE60706 RE72793 RH19208 RH19285 RH26759 RH27166 RH29766 RH36209 RH62890 RH68344
Other clones	75482 EC051540 EC051541 EC056098 EK045026 EK057620 EK115534 EK130554 EK139761 EK168154 EK229632 EK304346 EK307806 LD33381 RP003025789 RP003042390 RP003064947 RP003065279 RP003066781 RP003083188 RP003143084 RP003146408 RP003162414 RP003188277 RP003198910 RP003201914 RP003202558 RP003205503 RP003207614 RP003251591 RP003284354 RP003299596 RP003307194 RP003319597 RP003354529 RP003357546 RP003395296 RP003428842 RP003474505 RP003505486 RP003510670 RP003580270 RP003614732 RP003667495 RP003673724
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011737 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33965
Antibody Information
	polyclonal (Di Talia and Wieschaus, 2012, Garcia et al., 2009)
Other Information
Discoverer
Etymology
Identification
	In a screen for cDNA clones that rescue the S.pombe Spom\wee1 Spom\mik1 phenotype. (Campbell et al., 1995)
Relationship to Other Genes
Source for database identity of	Source for identity of: wee CG4488 (Wortman, 1999.11)
Source for database merge of
Additional comments	"S(CycEJP)2.8" is unlikely to correspond to "wee"; mutations in the two loci complement each other. (Brumby et al., 2004)
Other Comments
	When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells is seen. (Bettencourt-Dias et al., 2004) wee regulates cdc2 activity and times mitotic entry during the cortical nuclear cycles of syncytial blastoderm embryos. (Stumpff et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cell morphology is aberrant, indicative of a failure in cell cycle/mitosis progression. This phenotype is not seen in Kc167 cells. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) wee is zygotically dispensable but is required maternally for completing the nuclear division cycles of early embryogenesis. (Price et al., 2000) S.pombe Spom\wee1 Spom\mik1 double mutants are functionally complemented by wee. (Campbell et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057687 NM_001273238 RefSeq (Proteins) • NP_477035 NP_001260167 DDBJ / EMBL / GenBank • AA948877 AAC46913 AAF52453 AAM50802 AC004277 AC007977 AGB92703 AY118942 U17223 Entrez Gene - A searchable database of RefSeq genes. • 33965 UniProtKB/Swiss-Prot • P54350
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Protein kinase-like domain (IPR011009) Wee1-like protein kinase (IPR017164) Protein kinase, ATP binding site (IPR017441) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD27552
Linkouts
	BioGRID - A database of protein and genetic interactions • 60116 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011737 DroID - A comprehensive database of gene and protein interactions. • FBgn0011737 DRSC - Results from RNAi screens. • FBgn0011737 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011737 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4488-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011737 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33965 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/weekin1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33965 modMine - Data generated by the modENCODE project. • FBgn0011737 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P54350
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	CG4488 (Morrison et al., 2000, Keleman et al., 2009.8.5, Hughes et al., 2008, Ni et al., 2011.7.19) Dwee (Fichelson et al., 2005, Ji et al., 2002, Abdu et al., 2002, ) dwee1 (Stumpff et al., 2005, Stumpff et al., 2004, Reis and Edgar, 2004, Reis, 2003) Dwee1 (Fichelson and Gho, 2004, Lee and Orr-Weaver, 2003, Price et al., 2002, Reis and Edgar, 2002, Price et al., 2002, Silva et al., 2002, Campbell et al., 2001, Jin et al., 2001, Price et al., 2000, Price and Campbell, 2000, Vorbruggen et al., 2000, Vorbruggen et al., 2000, Price et al., 1998, Price et al., 1999, Campbell et al., 1996, Foe et al., 1993, Heriche et al., 2003, Radcliffe et al., 2002) dWee1 (Jin et al., 2003, Reis and Edgar, 2003, Garcia et al., 2009) Wee (Rubin et al., 2000) wee (Hueber et al., 2007, Fisher et al., 2008) Wee1 (Jin et al., 2005, Homola and Campbell, 2004, Campbell et al., 2000, Campbell et al., 1995, Hochwagen, 2006, Calzone et al., 2007, Di Talia and Wieschaus, 2012, Ninov et al., 2009, Glavic et al., 2009) wee1 (Brumby et al., 2004, Homola et al., 2001, Price and Campbell, 2001, Royou et al., 2008, Rickmyre et al., 2007, Jin et al., 2008, Rickmyre et al., 2007, Silva et al., 2006, Di Talia and Wieschaus, 2012) Wee 1 (O'Farrell, 2001) WEE1 (Morrison et al., 2000)
Name Synonym	wee
Secondary FlyBase IDs
	FBgn0010519
References ( 84 )
Generate a list of	All references relating to this gene ( 84 )
List References by type	Research paper  ( 36 ) Supplementary material  ( 8 ) Review  ( 6 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 17 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Website  ( 1 ) Poem  ( 1 )
Recent research papers ( 3 )
	Di Talia and Wieschaus, 2012, Dev. Cell 22(4): 763--774 Short-Term Integration of Cdc25 Dynamics Controls Mitotic Entry during Drosophila Gastrulation. [FBrf0218101] Fasulo et al., 2012, Mol. Biol. Cell 23(6): 1047--1057 Chk1 and Wee1 kinases coordinate DNA replication, chromosome condensation, and anaphase entry. [FBrf0217708] Oliveira et al., 2012, Molec. Phylog. Evol. 64(3): 533--544 Monophyly, divergence times, and evolution of host plant use inferred from a revised phylogeny of the Drosophila repleta species group. [FBrf0218731] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...