Dwee1, wee, Dwee
protein tyrosine kinase that targets Cdc2 thus preventing premature activation of the mitotic program - interacts with members of the γTubulin ring comples and is required for proper mitotic-spindle morphogenesis and positioning\
Please see the JBrowse view of Dmel\Wee1 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Gene model reviewed during 5.45
2.2 (compiled cDNA)
618 (aa); 69 (kD predicted)
Phosphorylated during M and G1 phases.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Wee1 using the Feature Mapper tool.
Comment: maternally deposited
Early maternal Wee1 expression is ubiquitous, and declines by gastrulation, with strong staining still detected in the pole cells. At stage 7, zygotic expression is detected in the presumptive mesoderm. After germ band retraction, Wee1 expression is detected in the gut and the CNS. CNS expression continues to be detected in late embryos and in first larval instar.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Wee1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for identity of: wee CG4488
Source for identity of: Wee1 wee
Symbol changed from 'wee' to 'Wee1' to reflect the preferred usage in the literature, including the initial paper (FBrf0083814), and all subsequent papers, to characterize the gene.
"S(CycEJP)2.8" is unlikely to correspond to "wee"; mutations in the two loci complement each other.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells is seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cell morphology is aberrant, indicative of a failure in cell cycle/mitosis progression. This phenotype is not seen in Kc167 cells.
wee is zygotically dispensable but is required maternally for completing the nuclear division cycles of early embryogenesis.
