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Gene Dmel\ana

General Information
Symbol	Dmel\ana	Species	D. melanogaster
Name	anachronism	Annotation symbol	CG8084
Feature type	protein_coding_gene	FlyBase ID	FBgn0011746
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	45A1-45A1	Sequence location	2R:4,949,800..4,965,059 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene anachronism is referred to in FlyBase by the symbol Dmel\ana (CG8084, FBgn0011746). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: growth factor activity. It is reported to be involved in the biological process: negative regulation of neuroblast proliferation. 17 alleles are reported. The phenotypes of these alleles are annotated with: neuroblast; thoracic neuroblast. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Transforming growth factor-beta, N-terminal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1630502_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of ana. Gene sequence location is 2R:4949800..4965059.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 45A1-45A1   Limits computationally determined from genome sequence between P{lacW}babok16912 and P{lacW}l(2)k16529k16529
Experimentally Determined Cytological Location
Cytogenetic map Notes References 45A-45B   (determined by in situ hybridisation)   (Ebens et al., 1993)
Experimentally Determined Recombination Data
Location	2-
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ana for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ana-RA FBtr0088559  NM_057690   2417   474 ana-RB FBtr0300501  NM_001169622   2426   477 ana-RC FBtr0339284     2072   390
Additional Transcript Data & Comments
Reported size (kB)	2.4, 1.7 (northern blot) (Ebens et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ana-PA   FBpp0087642   54.0   474   7.53     NP_477038   AAF58998 ana-PB   FBpp0289728   54.4   477   7.68     NP_001163093   ACZ94372 ana-PC   FBpp0308393   44.4   390   8.53
Additional Polypeptide Data & Comments
Reported size (kDa)	474 (aa); 55 (kD) (Ebens et al., 1993)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Transforming growth factor-beta, N-terminal (IPR001111)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58998   AC005458   AC006074   AE013599 ACZ94372   AI108915   AI113967   AI946583   AI946593   AW940261   AY060249 AAL25288   BI576257   BI612233   BI629574   BT132678   CO271336   CO277936   CO278569   CO279129   CO290811   CO300587   CO303385   CO316065   CO321222   CO329110   CO345571   CZ480085   EC061202   EC065653   EC198591   EC207877   EC220416   EC234664   EC254049   EC260111   GH941666   GH941803   GH942644   GH943093   GH945197   GH946030   GH946257   GH947068   GH947270   GH947728   GH950278   GH950409   GH952018   GH953705   GH954137   GH955601   GH961697   GH962836   GH963274   GH964381   GH964812   GH964914   GH965550   GH965715   GH965842   GH965945   GH966866   GH967178   GH967293   GH967948   GH969828   GH969881   GH970309   GH971761   GH972934   GH973456   GH973918   GH974294   GH975269   GH976378   GH978441   GH978840   GH978980   GH979011   GH979058   GH979092   GH979304   GH979832   GH980091   GH980470   GH980708   GH980738   GH980922   GH981868   GH982183   GH983266   GH984591   S63815 AAB27582
UniProtKB/Swiss-Prot	Q26307
UniProtKB/TrEMBL	E1JH14 Q95TB3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000436125 2R:4,949,440..4,954,247 evidence=Reporter construct (in vivo) (Hersh et al., 2007, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference larval stage   glial cell   (Ebens et al., 1993) embryonic/larval brain   (Ebens et al., 1993) embryonic/larval central nervous system | restricted   (Ebens et al., 1993) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 14 -- 17   presumptive embryonic/larval central nervous system | restricted   (Ebens et al., 1993) larval stage   optic lobe   (Ebens et al., 1993) ventral nerve cord   (Ebens et al., 1993)
Additional Descriptive Data
	ana transcripts are expressed in scattered cells throughout the larval CNS. Based on the distribution, morphology and nonneural identity (as demonstrated by double labeling with a neuron-specific marker), these cells are identified as glia. (Ebens et al., 1993)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference larval stage   glial cell   (Ebens et al., 1993) embryonic/larval brain   (Ebens et al., 1993) embryonic/larval central nervous system | restricted   (Ebens et al., 1993) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 14 -- 17   presumptive embryonic/larval central nervous system | restricted   (Ebens et al., 1993) larval stage   optic lobe   (Ebens et al., 1993) ventral nerve cord   (Ebens et al., 1993)
Additional Descriptive Data
	ana protein is localized to vesicles in expressing cells. (Ebens et al., 1993)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    195.05 Larval Midgut    53.2 Larval Hindgut    17.8 Larval Malpighian Tubules    6.4 Larval Fat Body    5.8 Larval Salivary Gland    5.5 Larval Trachea    7.325 Larval Carcass    25 Adult Head    190.3 Adult Eye    31.825 Adult Brain    437 Adult Thoracic-Abdominal Ganglion    565.7 Adult Crop    13.3 Adult Midgut    16 Adult Hindgut    8.6 Adult Malpighian Tubules    3.9 Adult Fat Body    24.3 Adult Salivary Gland    17.2 Adult Heart    34.375 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.2 Adult Ovary    70.9 Adult Testis    635 Adult Male Accessory Gland    11.3 Adult Carcass    22.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    195.05 Larval Midgut    53.2 Larval Hindgut    17.8 Larval Malpighian Tubules    6.4 Larval Fat Body    5.8 Larval Salivary Gland    5.5 Larval Trachea    7.325 Larval Carcass    25 Adult Head    190.3 Adult Eye    31.825 Adult Brain    437 Adult Thoracic-Abdominal Ganglion  (565.7) Adult Crop    13.3 Adult Midgut    16 Adult Hindgut    8.6 Adult Malpighian Tubules    3.9 Adult Fat Body    24.3 Adult Salivary Gland    17.2 Adult Heart    34.375 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.2 Adult Ovary    70.9 Adult Testis  (635) Adult Male Accessory Gland    11.3 Adult Carcass    22.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    195.05 Larval Midgut    53.2 Larval Hindgut    17.8 Larval Malpighian Tubules    6.4 Larval Fat Body    5.8 Larval Salivary Gland    5.5 Larval Trachea    7.325 Larval Carcass    25 Adult Head    190.3 Adult Eye    31.825 Adult Brain    437 Adult Thoracic-Abdominal Ganglion    565.7 Adult Crop    13.3 Adult Midgut    16 Adult Hindgut    8.6 Adult Malpighian Tubules    3.9 Adult Fat Body    24.3 Adult Salivary Gland    17.2 Adult Heart    34.375 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.2 Adult Ovary    70.9 Adult Testis    635 Adult Male Accessory Gland    11.3 Adult Carcass    22.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    195.05 Larval Midgut    53.2 Larval Hindgut    17.8 Larval Malpighian Tubules    6.4 Larval Fat Body    5.8 Larval Salivary Gland    5.5 Larval Trachea    7.325 Larval Carcass    25 Adult Head    190.3 Adult Eye    31.825 Adult Brain    437 Adult Thoracic-Abdominal Ganglion    565.7 Adult Crop    13.3 Adult Midgut    16 Adult Hindgut    8.6 Adult Malpighian Tubules    3.9 Adult Fat Body    24.3 Adult Salivary Gland    17.2 Adult Heart    34.375 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.2 Adult Ovary    70.9 Adult Testis    635 Adult Male Accessory Gland    11.3 Adult Carcass    22.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    195.05 Larval Midgut    53.2 Larval Hindgut    17.8 Larval Malpighian Tubules    6.4 Larval Fat Body    5.8 Larval Salivary Gland    5.5 Larval Trachea    7.325 Larval Carcass    25 Adult Head    190.3 Adult Eye    31.825 Adult Brain    437 Adult Thoracic-Abdominal Ganglion    565.7 Adult Crop    13.3 Adult Midgut    16 Adult Hindgut    8.6 Adult Malpighian Tubules    3.9 Adult Fat Body    24.3 Adult Salivary Gland    17.2 Adult Heart    34.375 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.2 Adult Ovary    70.9 Adult Testis    635 Adult Male Accessory Gland    11.3 Adult Carcass    22.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    195.05 Larval Midgut    53.2 Larval Hindgut    17.8 Larval Malpighian Tubules    6.4 Larval Fat Body    5.8 Larval Salivary Gland    5.5 Larval Trachea    7.325 Larval Carcass    25 Adult Head    190.3 Adult Eye    31.825 Adult Brain    437 Adult Thoracic-Abdominal Ganglion  565.7 Adult Crop    13.3 Adult Midgut    16 Adult Hindgut    8.6 Adult Malpighian Tubules    3.9 Adult Fat Body    24.3 Adult Salivary Gland    17.2 Adult Heart    34.375 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.2 Adult Ovary    70.9 Adult Testis  635 Adult Male Accessory Gland    11.3 Adult Carcass    22.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    195.05 Larval Midgut    53.2 Larval Hindgut    17.8 Larval Malpighian Tubules    6.4 Larval Fat Body    5.8 Larval Salivary Gland    5.5 Larval Trachea    7.325 Larval Carcass    25 Adult Head    190.3 Adult Eye    31.825 Adult Brain    437 Adult Thoracic-Abdominal Ganglion    565.7 Adult Crop    13.3 Adult Midgut    16 Adult Hindgut    8.6 Adult Malpighian Tubules    3.9 Adult Fat Body    24.3 Adult Salivary Gland    17.2 Adult Heart    34.375 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.2 Adult Ovary    70.9 Adult Testis    635 Adult Male Accessory Gland    11.3 Adult Carcass    22.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    195.05 Larval Midgut    53.2 Larval Hindgut    17.8 Larval Malpighian Tubules    6.4 Larval Fat Body    5.8 Larval Salivary Gland    5.5 Larval Trachea    7.325 Larval Carcass    25 Adult Head    190.3 Adult Eye    31.825 Adult Brain    437 Adult Thoracic-Abdominal Ganglion    565.7 Adult Crop    13.3 Adult Midgut    16 Adult Hindgut    8.6 Adult Malpighian Tubules    3.9 Adult Fat Body    24.3 Adult Salivary Gland    17.2 Adult Heart    34.375 Adult VirginFemale Spermatheca    11.5 Adult InseminatedFemale Spermatheca    10.2 Adult Ovary    70.9 Adult Testis    635 Adult Male Accessory Gland    11.3 Adult Carcass    22.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    190.3   NA  Eye    31.825   NA  Brain    437   195.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    565.7   NA  Crop    13.3   53.2  Midgut    16   17.8  Hindgut    8.6   6.4  Malpighian Tubules    3.9   5.8  Fat Body    24.3   5.5  Salivary Gland    no informative data   NA  Heart    34.375   7.325  Trachea    NA   NA  VirginFemale Spermatheca    11.5   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    70.9   NA  Testis    635   NA  Male Accessory Gland    no informative data   25  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    8 central nervous system, pupae P8    7 head, virgin 1-day female    8 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    17 head, mated 4-day male    20 head, mated 20-day male    13 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    4 testis, mated 4-day male    51 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (30) central nervous system, larvae L3    8 central nervous system, pupae P8    7 head, virgin 1-day female    8 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    17 head, mated 4-day male    20 head, mated 20-day male    13 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    4 testis, mated 4-day male  (51) accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    8 central nervous system, pupae P8    7 head, virgin 1-day female    8 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    17 head, mated 4-day male    20 head, mated 20-day male    13 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    4 testis, mated 4-day male    51 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    8 central nervous system, pupae P8    7 head, virgin 1-day female    8 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    17 head, mated 4-day male    20 head, mated 20-day male    13 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    4 testis, mated 4-day male    51 accessory gland, mated 4-day male    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    8 central nervous system, pupae P8    7 head, virgin 1-day female    8 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    17 head, mated 4-day male    20 head, mated 20-day male    13 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    4 testis, mated 4-day male    51 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  30 central nervous system, larvae L3    8 central nervous system, pupae P8    7 head, virgin 1-day female    8 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    17 head, mated 4-day male    20 head, mated 20-day male    13 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    4 testis, mated 4-day male  (51) accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    8 central nervous system, pupae P8    7 head, virgin 1-day female    8 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    17 head, mated 4-day male    20 head, mated 20-day male    13 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    4 testis, mated 4-day male    51 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    8 central nervous system, pupae P8    7 head, virgin 1-day female    8 head, virgin 4-day female    10 head, virgin 20-day female    6 head, mated 1-day female    11 head, mated 4-day female    10 head, mated 20-day female    7 head, mated 1-day male    17 head, mated 4-day male    20 head, mated 20-day male    13 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    2 ovary, mated 4-day female    4 testis, mated 4-day male    51 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    10 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    6 white prepupae new    4 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    9 adult female 01day    5 adult female 05day    4 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    10 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    6 white prepupae new    4 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    9 adult female 01day    5 adult female 05day    4 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    10 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    6 white prepupae new    4 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    9 adult female 01day    5 adult female 05day    4 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    10 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    6 white prepupae new    4 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    9 adult female 01day    5 adult female 05day    4 adult female 30day    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    10 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    6 white prepupae new    4 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    9 adult female 01day    5 adult female 05day    4 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    10 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    6 white prepupae new    4 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    9 adult female 01day    5 adult female 05day    4 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    10 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    6 white prepupae new    4 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    9 adult female 01day    5 adult female 05day    4 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    3 embryo 08-10hr    3 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    5 embryo 16-18hr    6 embryo 18-20hr    10 embryo 20-22hr    7 embryo 22-24hr    9 larva L1    7 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    6 white prepupae new    4 white prepupae 12hr    3 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    9 adult female 01day    5 adult female 05day    4 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    0 embryonic S1    2 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    0 embryonic S1    2 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    0 embryonic S1    2 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    0 embryonic S1    2 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    0 embryonic S1    2 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    0 embryonic S1    2 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    0 embryonic S1    2 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    0 embryonic S1    2 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    4 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_24 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011746 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8084-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable ana1 ana8 ana9 Other Phenotypes Allele smell perception defective | recessive ana1 ana9 wild-type ana+mEa ana+t9.3 Phenotype manifest in Allele neuroblast ana1 optic lobe & neuroblast ana8 thoracic neuroblast ana8 type I neuroblast | somatic clone | third instar larval stage anaLA00274
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of ana Class Mutagen Stocks Known lesion anaLA00274 2 -- ana1 loss of function allele Delta2-3 P-element activity 1 Yes anae02258 piggyBac transposase 1 -- ana8 loss of function allele, amorphic allele - genetic evidence Delta2-3 0 Yes ana2 loss of function allele Delta2-3 0 Yes ana3 loss of function allele Delta2-3 0 Yes ana4 loss of function allele Delta2-3 0 Yes ana5 loss of function allele Delta2-3 0 Yes ana6 loss of function allele Delta2-3 0 Yes ana7 hypomorphic allele - genetic evidence Delta2-3 0 Yes ana9 loss of function allele Delta2-3 0 Yes anaDG12701 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ana Class Mutagen Stocks Known lesion ana+mEa in vitro construct - minigene in vitro construct - genomic fragment 1 Yes anaJF02665 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ana+t9.3 in vitro construct - genomic fragment 0 Yes anaGD5775 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes anahs.P in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Np1 (Ebens et al., 1993) Df(2R)Np6 (Ebens et al., 1993)
Not disrupted in	Df(2R)w45-31n (Ebens et al., 1993) Df(2R)w45-47n (Ebens et al., 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5775} NA P{TRiP.JF02665} NA heat-shock construct P{anahs.P} NA characterization construct P{ana+9.3} NA P{ana+m} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{wHy}anaDG12701 NA PBac{RB}anae02258 NA insertion of mobile activating element P{Mae-UAS.6.11}anaLA00274 NA insertion of enhancer trap P{A92}ana1 No
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
growth factor activity inferred from electronic annotation with InterPro:IPR001111 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
growth inferred from electronic annotation with InterPro:IPR001111 (FlyBase Curators et al., 2004-) negative regulation of neuroblast proliferation non-traceable author statement (Swiss-Prot Project Members, 2000.10.1)
Cellular Component
CV term Evidence References
extracellular region non-traceable author statement (Swiss-Prot Project Members, 2000.10.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ana allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61751 DroID - A comprehensive database of gene and protein interactions. • FBgn0011746 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35913
Orthologs
OrthoDB Orthologs (12) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG65HSHJ Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG65HSHJ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\ana     Drosophila simulans   Dsim\GD10624 Y   Drosophila sechellia   Dsec\GM21087 Y   Drosophila erecta   Dere\GG23405 Y   Drosophila yakuba   Dyak\GE19247 Y   Drosophila ananassae   Dana\GF12224 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20811 Y   Drosophila persimilis   Dper\GL17613 Y   Drosophila willistoni   Dwil\GK17993 Y   Drosophila virilis   Dvir\GJ19855 Y   Drosophila mojavensis   Dmoj\GI20089 Y   Drosophila grimshawi   Dgri\GH21635 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10624 Drosophila sechellia Dsec\GM21087 Drosophila erecta Dere\GG23405 Drosophila yakuba Dyak\GE19247 Drosophila ananassae Dana\GF12224 Drosophila pseudoobscura pseudoobscura Dpse\GA20811 Drosophila persimilis Dper\GL17613 Drosophila willistoni Dwil\GK17993 Drosophila virilis Dvir\GJ19855 Drosophila mojavensis Dmoj\GI20089 Drosophila grimshawi Dgri\GH21635
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	27515 y1 v1; P{TRiP.JF02665}attP2 8927 P{ana+m}1, w*; P{A92}ana1 22173 y1 w*; P{Mae-UAS.6.11}anaLA00274
Harvard	- PBac{RB}anae02258
Kyoto	120274 y* w*; P{Mae-UAS.6.11}anaLA00274
Genomic Clones ( 2 )
	BACR08E17 BACR10M14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 83 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH07389 RH40649
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH10527 RH32783 RH58463
Other clones	16219 18647 57686 121147 214223.35 214449.44 bs28a11 bs28c03 CK01.97A.B6 CK01.167C.H4 EK075230 EK103062 EK127839 EK130431 EK133605 EK178642 EK241232 EK263602 EK290530 EK292040 EP22311 RP004093783 RP004119303 RP004125879 RP004150325 RP004171948 RP004173976 RP004196939 RP004207052 RP004210687 RP004212165 RP004213240 RP004222681 RP004224389 RP004225382 RP004226634 RP004234756 RP004234778 RP004238446 RP004238618 RP004240925 RP004245159 RP004245315 RP004251787 RP004252334 RP004254665 RP004255037 RP004255125 RP004256828 RP004256974 RP004259899 RP004262171 RP004264458 RP004266550 RP004320698 RP004346889 RP004366248 RP004373195 RP004381196 RP004389623 RP004407712 RP004428855 RP004434169 RP004436186 RP004461276 RP004469836 RP004471751 RP004475074 RP004485670 RP004486763 RP004489297 RP004490592 RP004497900 RP004499009 RP004504506 RP004517351 RP004521141 RP004534422
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011746 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35913
Antibody Information
	monoclonal, polyclonal (Ebens et al., 1993)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB. (Liu and Lehmann, 2008) Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. (Claridge-Chang et al., 2001) trol acts downstream of ana, it is possible that trol may function to inactivate or bypass ana repression to activate cell proliferation. (Datta, 1995) ana encodes a secreted glial glycoprotein that represses neuroblast proliferation in the larval brain. (Datta, 1995) The ana locus controls the proliferation of neuroblasts, but is expressed in glia. In mutants, quiescent neuroblasts enter S phase precociously. (Ebens et al., 1993) Required for neuroblast proliferation but expressed in glial cells.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057690 NM_001169622 RefSeq (Proteins) • NP_477038 NP_001163093 DDBJ / EMBL / GenBank • AAB27582 AAF58998 AAL25288 AC005458 AC006074 ACZ94372 AI108915 AI946583 AI946593 AW940261 AY060249 BI629574 CZ480085 S63815 Entrez Gene - A searchable database of RefSeq genes. • 35913 UniProtKB/Swiss-Prot • Q26307 UniProtKB/TrEMBL • E1JH14 Q95TB3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Transforming growth factor-beta, N-terminal (IPR001111)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61751 DroID - A comprehensive database of gene and protein interactions. • FBgn0011746 DRSC - Results from RNAi screens. • FBgn0011746 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011746 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8084-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011746 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35913 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /neural/anacron.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35913 modMine - Data generated by the modENCODE project. • FBgn0011746 REDfly - A database of transcriptional regulatory elements. • FBgn0011746
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	ana (Miller et al., 2009, Barmina et al., 2005, Jacobsen et al., 2006, Weng and Cohen, 2012, Hueber et al., 2007, Liu and Lehmann, 2008, Hersh et al., 2007, Rebeiz et al., 2011, Yakoby et al., 2008, Kadener et al., 2009, Fernández-Ayala et al., 2010, Awofala et al., 2012) CG8084 (Perkins et al., 2009, Awofala et al., 2012) med (Ebens et al., 1991, )
Name Synonym	anachronism (Barmina et al., 2005, Weng and Cohen, 2012, Hersh et al., 2007, Harbison et al., 2005, Cirelli et al., 2005, Ou et al., 2011) Anachronism (Awofala et al., 2012) medullaless
Secondary FlyBase IDs
References ( 55 )
Generate a list of	All references relating to this gene ( 55 )
List References by type	Research paper  ( 22 ) Supplementary material  ( 4 ) Review  ( 9 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 8 ) FlyBase analysis  ( 2 ) Protein sequence record  ( 1 )
Recent research papers ( 4 )
	Awofala et al., 2012, Funct. Integr. Genomics 12(2): 305--315 Functional roles for redox genes in ethanol sensitivity in Drosophila. [FBrf0218408] Weng and Cohen, 2012, Development 139(8): 1427--1434 Drosophila miR-124 regulates neuroblast proliferation through its target anachronism. [FBrf0217785] Ou et al., 2011, PLoS Biol. 9(9): e1001160 Nuclear Receptor DHR4 Controls the Timing of Steroid Hormone Pulses During Drosophila Development. [FBrf0216318] Rebeiz et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(25): 10036--10043 Evolutionary origin of a novel gene expression pattern through co-option of the latent activities of existing regulatory sequences. [FBrf0214016] Show all research papers ...
Recent reviews ( 1 )
	Broadie et al., 2011, Dev. Neurobiol. 71(11): 1102--1130 Extracellular matrix and its receptors in drosophila neural development. [FBrf0216377] Show all reviews ...