FB2020_01, released Feb 12, 2020

Gene: Dmel\ana

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General Information
Symbol
Dmel\ana
Species
D. melanogaster
Name
anachronism
Annotation Symbol
CG8084
Feature Type
protein_coding_gene
FlyBase ID
FBgn0011746
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Snapshot
anachronism (ana) encodes a secreted glycoprotein that is expressed in glia and at lower levels in neuroblasts. It suppresses neuroblast proliferation. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

med

Key Links
Genomic Location
Cytogenetic map
45A1-45A1
Sequence location
2R:9,062,295..9,077,554 [+]
Recombination map

2-60

RefSeq locus
NT_033778 REGION:9062295..9077554
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Molecular Function (GO)
    [Detailed GO annotations]
    Experimental Evidence
    -
    Predictions / Assertions
    -
    Summaries
    Protein Function (UniProtKB)
    Negatively regulates proliferation of neuronal precursor cells, thereby controlling the timing of postembryonic neurogenesis.
    (UniProt, Q26307)
    Summary (Interactive Fly)
    Gene Model and Products
    Number of Transcripts
    3
    Number of Unique Polypeptides
    3

    Please see the GBrowse view of Dmel\ana or the JBrowse view of Dmel\ana for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Low-frequency RNA-Seq exon junction(s) not annotated.

    Gene model reviewed during 5.49

    Sequence Ontology: Class of Gene
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    ana-RA
    FBtr0088559
    NM_057690
    2417
    474
    ana-RB
    FBtr0300501
    NM_001169622
    2426
    477
    ana-RC
    FBtr0339284
    NM_001299297
    2072
    390
    Additional Transcript Data and Comments
    Reported size (kB)

    2.4, 1.7 (northern blot)

    (Ebens et al., 1993)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    ana-PA
    FBpp0087642
    54.0
    474
    7.53
    NP_477038
    AAF58998
    ana-PB
    FBpp0289728
    54.4
    477
    7.68
    NP_001163093
    ACZ94372
    ana-PC
    FBpp0308393
    44.4
    390
    8.53
    NP_001286226
    AHN56024
    Polypeptides with Identical Sequences

    None of the polypeptides share 100% sequence identity.

    Additional Polypeptide Data and Comments
    Reported size (kDa)

    474 (aa); 55 (kD)

    (Ebens et al., 1993)
    Comments
    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ana using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Gene Ontology (0 terms)
    Molecular Function (0 terms)
    Terms Based on Experimental Evidence (0 terms)
    Terms Based on Predictions or Assertions (0 terms)
    Biological Process (0 terms)
    Terms Based on Experimental Evidence (0 terms)
    Terms Based on Predictions or Assertions (0 terms)
    Cellular Component (0 terms)
    Terms Based on Experimental Evidence (0 terms)
    Terms Based on Predictions or Assertions (0 terms)
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    No Assay Recorded
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    larval stage
    glial cell
    (Ebens et al., 1993)
    embryonic/larval brain
    (Ebens et al., 1993)
    embryonic/larval central nervous system | restricted
    (Ebens et al., 1993)
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 14 -- 17
    presumptive embryonic/larval central nervous system | restricted
    (Ebens et al., 1993)
    larval stage
    adult optic lobe
    (Ebens et al., 1993)
    ventral nerve cord
    (Ebens et al., 1993)
    third instar larval stage
    wing pouch | restricted
    (Hersh et al., 2007)
    mesothoracic tergum | restricted
    (Hersh et al., 2007)
    Additional Descriptive Data

    ana is expressed in four spots in the wing pouch and an additional spot in the notum.

    (Hersh et al., 2007)

    ana transcripts are expressed in scattered cells throughout the larval CNS. Based on the distribution, morphology and nonneural identity (as demonstrated by double labeling with a neuron-specific marker), these cells are identified as glia.

    (Ebens et al., 1993)
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    No Assay Recorded
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    larval stage
    glial cell
    (Ebens et al., 1993)
    embryonic/larval brain
    (Ebens et al., 1993)
    embryonic/larval central nervous system | restricted
    (Ebens et al., 1993)
    immunolocalization
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    larval stage
    adult optic lobe
    (Ebens et al., 1993)
    ventral nerve cord
    (Ebens et al., 1993)
    embryonic stage 14 -- 17
    presumptive embryonic/larval central nervous system | restricted
    (Ebens et al., 1993)
    Additional Descriptive Data

    ana protein expression occurs in scattered cells throughout the larval CNS. Based on the distribution, morphology and nonneural identity (as demonstrated by double labeling with a neuron-specific marker), these cells are identified as glia.

    (Ebens et al., 1993)
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    Expression Deduced from Reporters
    Reporter: P{ana-spot.GFP}
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    third instar larval stage
    wing pouch | restricted
    (Hersh et al., 2007)
    mesothoracic tergum | restricted
    (Hersh et al., 2007)
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\ana in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 13 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 6 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of ana
    Transgenic constructs containing regulatory region of ana
    Deletions and Duplications ( 4 )
    Phenotypes
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190ARG )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Dmel\ana
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00003303
    Drosophila simulans
    Dsim\GD10624
    Drosophila sechellia
    Dsec\GM21087
    Drosophila erecta
    Dere\GG23405
    Drosophila yakuba
    Dyak\GE19247
    Drosophila ananassae
    Dana\GF12224
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA20811
    Drosophila persimilis
    Dper\GL17613
    Drosophila willistoni
    Dwil\GK17993
    Drosophila virilis
    Dvir\GJ19855
    Drosophila mojavensis
    Dmoj\GI20089
    Drosophila grimshawi
    Dgri\GH21635
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AIY )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Mdoa\17017234
    Glossina morsitans
    Tsetse fly
    Gmor\GMOY010966
    Mayetiola destructor
    Hessian fly
    Mdes\Mdes005527
    Aedes aegypti
    Yellow fever mosquito
    Aaeg\AAEL007208
    Anopheles darlingi
    American malaria mosquito
    Adar\ADAC005341
    Anopheles gambiae
    Malaria mosquito
    Agam\AGAP005987
    Culex quinquefasciatus
    Southern house mosquito
    Cqui\CPIJ010830
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0BWT )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Ixodes scapularis
    Black-legged tick
    Isca\ISCW009992
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v7.1)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      RNA-RNA
      Physical Interaction
      Assay
      References
      ana - mir-124
      quantitative reverse transcription pcr, luminiscence technology, necessary binding region
      (Weng and Cohen, 2012)
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      mir-124
      suppressible
      ana
      (Weng and Cohen, 2012)
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2R
      Recombination map

      2-60

      Cytogenetic map
      45A1-45A1
      Sequence location
      2R:9,062,295..9,077,554 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      45A1-45A1
      Limits computationally determined from genome sequence between P{lacW}babok16912 and P{lacW}l(2)k16529k16529
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      45A-45B
      (determined by in situ hybridisation)
      (Ebens et al., 1993)
      Experimentally Determined Recombination Data
      Location

      2-60

      (FlyBase, 2016)

      2-58.1

      (Comeron et al., 2012)

      2-

      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (5)
      Genomic Clones (28)
      cDNA Clones (83)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        monoclonal, polyclonal

        (Ebens et al., 1993)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments
        Other Comments

        Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB.

        (Liu and Lehmann, 2008)

        Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

        (Claridge-Chang et al., 2001)

        ana encodes a secreted glial glycoprotein that represses neuroblast proliferation in the larval brain.

        (Datta, 1995)

        trol acts downstream of ana, it is possible that trol may function to inactivate or bypass ana repression to activate cell proliferation.

        (Datta, 1995)

        The ana locus controls the proliferation of neuroblasts, but is expressed in glia. In mutants, quiescent neuroblasts enter S phase precociously.

        (Ebens et al., 1993)

        Required for neuroblast proliferation but expressed in glial cells.

        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 43 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Synonyms and Secondary IDs (7)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (75)