dDP, l(2)vr10, DP1
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
1.2 (longest cDNA)
None of the polypeptides share 100% sequence identity.
Heterodimer of E2f and Dp. Cooperate to give sequence-specific DNA binding and optimal trans-activation. Component of the DREAM complex at least composed of Myb, Caf1-55, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, HDAC1/Rpd3 and l(3)mbt.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dp using the Feature Mapper tool.
In syncytial embryos, Dp transcripts are clumped around migrating nuclei. Upon cellularization, they become localized below the nuclei. In early gastrulation, Dp transcripts are localized fairly uniformly and at later stages of germ band extension, they are apparent mainly in regions with dividing cells. In later embryonic stages, Dp expression is confined to the nervous system. Dp transcripts are restricted to proliferating cells in later developmental stages as well. Dp transcripts are observed in the third instar larval brain Dp transcripts are observed in the optic lobe and in the ventral ganglion. They are observed in leg discs and in antennal discs. In eye discs, expression is observed anterior to the morphogenetic furrow, in a stripe just behind it, and at a lower level over the rest of the disc. Expression is also observed in the testis through the spermatocyte growing stages.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Dp in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell size is increased, microtubules are uniform or disorganised, cell shape is irregular, and cell number is decreased indicative of a failure in cell cycle progression through G1 to S and G2 to M stages.
Dp is required for several essential processes during oogenesis, including dorsal-ventral patterning and germline cell cycle control.
Identification: One of a collection of genes identified with defective larval growth that extend larval life.
Both Dp and E2f are necessary for viability and mutations in the genes cause lethality at the larval\pupal stage. Dp is required for normal development in both mitotic and endo cycle cells. Mutant phenotypes reveal that both genes promote progression of the cell cycle.
In vitro binding studies of E2f and Dp to the DNApol-α180 promoter region reveals each of the E2f binding sites plays a distinct role as positive or negative element in the regulation of the DNApol-α180 promoter during development.
The Dp gene product is preferentially expressed in proliferating cells. Distinct expression patterns of Dp and E2f suggest that the formation of Dp/E2f heterodimers is subject to complex regulatory cues.