Open Close
General Information
Symbol
Dmel\E2f1
Species
D. melanogaster
Name
E2F transcription factor 1
Annotation Symbol
CG6376
Feature Type
FlyBase ID
FBgn0011766
Gene Model Status
Stock Availability
Gene Summary
Transcriptional activator that binds to E2f sites. Required for wild-type growth in mitotic and polytene tissues, Contributes to the expression of replication genes at the G1-S transition and Cyclin E. Activates cell proliferation in wing imaginal disk, which requires expression of vg. (UniProt, Q27368)
Contribute a Gene Snapshot for this gene.
Also Known As

E2F, dE2F1, dE2F, E(var)3-93E, drosE2F1

Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:21,620,304..21,660,871 [-]
Recombination map
3-72
RefSeq locus
NT_033777 REGION:21620304..21660871
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (29 terms)
Molecular Function (11 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
enables DNA binding
inferred from direct assay
inferred from physical interaction with FLYBASE:l(3)mbt; FB:FBgn0002441
inferred from physical interaction with FLYBASE:dom; FB:FBgn0020306
inferred from physical interaction with FLYBASE:Rbf; FB:FBgn0015799
inferred from physical interaction with FLYBASE:Dp; FB:FBgn0011763
inferred from physical interaction with FLYBASE:Dp; FB:FBgn0011763
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with HGNC:3113
inferred from electronic annotation with InterPro:IPR032198
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000249624
(assigned by GO_Central )
Biological Process (15 terms)
Terms Based on Experimental Evidence (12 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:CycE; FB:FBgn0010382
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from high throughput mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000249624
(assigned by GO_Central )
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000249624
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the E2F/DP family. (Q27368)
Summaries
Gene Group (FlyBase)
E2F TRANSCRIPTION FACTORS -
The E2F/DP family of transcription factors are sequence-specific DNA-binding proteins that regulate transcription. The functional E2F are transcriptional heterodimers composed of two subunits, E2F and DP. (Adapted from FBrf0128583).
Protein Function (UniProtKB)
Transcriptional activator that binds to E2f sites. Required for wild-type growth in mitotic and polytene tissues, Contributes to the expression of replication genes at the G1-S transition and Cyclin E. Activates cell proliferation in wing imaginal disk, which requires expression of vg.
(UniProt, Q27368)
Summary (Interactive Fly)

positively regulates genes involved in the S (DNA synthesis) phase of the cell cycle - alternate transcripts are necessary for maintenance of cell cycle exit during development - required during late myogenesis to directly control the expression of a set of muscle-specific genes

Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\E2f1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.56

Gene model reviewed during 5.47

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.55

Gene model reviewed during 6.02

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0084119
3913
805
FBtr0084117
3150
805
FBtr0084118
4778
805
FBtr0334678
3581
821
FBtr0345217
4234
821
FBtr0346157
4653
805
Additional Transcript Data and Comments
Reported size (kB)

4.7 (northern blot)

4.5 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0083518
87.5
805
6.64
FBpp0083516
87.5
805
6.64
FBpp0083517
87.5
805
6.64
FBpp0306739
89.3
821
6.72
FBpp0311412
89.3
821
6.72
FBpp0311985
87.5
805
6.64
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

805 aa isoforms: E2f1-PA, E2f1-PB, E2f1-PC, E2f1-PF
821 aa isoforms: E2f1-PD, E2f1-PE
Additional Polypeptide Data and Comments
Reported size (kDa)

805 (aa); 88 (kD predicted)

Comments

E2f2 protein requires Dp protein for binding to the E2f recognition sites of the adenovirus E2 promoter. E2f protein does not require Dp protein for binding the same site, but binding is enhanced in the presence of Dp protein. E2f2 binds to one of two E2f recognition sites in the mus209 gene promoter. Unlike the transcription activator E2f, E2f2 acts as a repressor of expression from the mus209 promoter.

Cotransfection assays in S2 cells were used to demonstrate that E2f protein and Dp protein form a heteromeric complex and cooperate to give sequence-specific DNA binding and transcriptional activation. Deletion mutants of E2f protein were made to identify a region of E2f protein necessary for interaction with Dp protein.

E2f protein expressed in bacteria was shown to have DNA-binding activity and to bind specifically to E2f recognition sequences. In S2 cells, E2f protein stimulates transcription and the activation is dependent on functional E2f binding sites. The transcription activation function was mapped to the C-terminal portion of the E2f protein between amino acids 560 and 769. Finally, E2f protein recognition sequences were found within the promoter of the DNA polymerase alpha gene.

External Data
Subunit Structure (UniProtKB)

Heterodimer of E2f and Dp. Cooperates to give sequence-specific DNA binding and optimal trans-activation. Interacts with PCNA.

(UniProt, Q27368)
Post Translational Modification

Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase. Ubiquitination by the DCX(DTL) complex is essential for cell cycle control and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.

(UniProt, Q27368)
Domain

The PIP-box K+4 motif mediates both the interaction with PCNA and the recruitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.

(UniProt, Q27368)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\E2f1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

antennal anlage

Comment: reported as procephalic ectoderm anlage

central brain anlage

Comment: reported as procephalic ectoderm anlage

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

visual anlage

Comment: reported as procephalic ectoderm anlage

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

antennal primordium

Comment: reported as procephalic ectoderm primordium

central brain primordium

Comment: reported as procephalic ectoderm primordium

visual primordium

Comment: reported as procephalic ectoderm primordium

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\E2f1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 56 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 41 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of E2f1
Transgenic constructs containing regulatory region of E2f1
Aberrations (Deficiencies and Duplications) ( 6 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
embryonic thorax & neuron
eye disc & mitotic cell cycle
heterochromatin & nurse cell, with E2f191
heterochromatin & nurse cell, with E2f1hs.PD
heterochromatin & nurse cell (with Df(3R)e-BS2)
midgut primordium & S phase
nuclear chromosome & nurse cell (with Df(3R)e-BS2)
nuclear membrane & nurse cell (with Df(3R)e-BS2)
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
1  
9 of 15
Yes
Yes
2  
8 of 15
No
Yes
1  
5 of 15
No
Yes
4 of 15
No
No
1  
3 of 15
No
No
1  
1 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
9 of 15
Yes
Yes
8 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (8)
6 of 13
Yes
Yes
6 of 13
Yes
Yes
5 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
No
2 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (7)
5 of 12
Yes
Yes
5 of 12
Yes
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (8)
7 of 15
No
Yes
3 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
5 of 15
Yes
Yes
4 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (3)
5 of 9
Yes
Yes
5 of 9
Yes
Yes
3 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909BJ )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091509L2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0ANC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0AK1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
4 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 5 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    RNA-RNA
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    suppressible
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Heterodimer of E2f and Dp. Cooperates to give sequence-specific DNA binding and optimal trans-activation. Interacts with PCNA.
    (UniProt, Q27368 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-72
    Cytogenetic map
    Sequence location
    3R:21,620,304..21,660,871 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    93E9-93F1
    Limits computationally determined from genome sequence between P{PZ}InR05545 and P{PZ}tsl00617
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    93E8-93E9
    (determined by in situ hybridisation)
    93E-93E
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (36)
    Genomic Clones (29)
    cDNA Clones (108)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: E2f1 E2f

      Source for database merge of
      Additional comments

      'E2f' renamed to 'E2f1' to: i) reflect usage in the literature; ii) better distinguish it from the 'E2f2' gene; iii) reflect preference of authors Dyson and Duronio who originally characterized the gene in FBrf0073014 and FBrf0081967.

      mir-998 and mir-11 are both located in the same intron of E2f.

      mir-998 is located within an intron of E2f.

      Other Comments

      dsRNA has been made from templates generated with primers directed against this gene. RNAi of E2f results in increased arborization of ddaD and ddaE neurons. RNAi also causes defects in muscle, alterations in the number of MD neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.

      RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.

      RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

      RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

      E2f as part of the E2f-Rb complexes has a role in the down regulation of CycE during the gap phase of endocycling cells in normal development.

      E2f transcriptional activity in nurse cells is essential for thr robust synthesis of S phase transcripts that are deposited into the oocyte. E2f is needed to limit the replication of heterochromatin in nurse cells. E2f is required for nurse cell nuclear envelope breakdown and apoptosis.

      Identified with: LP10386 (BDGP-DGC) <up>FlyBase curator comment: EST subsequently found to be chimeric</up>.

      Most, if not all, of the function of Rbf during development is mediated through E2f. E2f functions primarily as a transcription activator rather than a co-repressor of Rbf during development.

      Identification: One of a collection of genes identified with defective larval growth that extend larval life.

      Both Dp and E2f are necessary for viability and mutations in the genes cause lethality at the larval\pupal stage. E2f is required for wild-type growth in mitotic and polytene tissues. Mutant phenotypes reveal that both genes promote progression of the cell cycle.

      In vitro binding studies of E2f and Dp to the DNApol-α180 promoter region reveals each of the E2f binding sites plays a distinct role as positive or negative element in the regulation of the DNApol-α180 promoter during development.

      Endogenous E2f falls from high to very low levels as cells initiate DNA synthesis during a developmentally regulated G1-S transition in the eye disc. Ectopic E2f expression drives many otherwise quiescent cells to enter S phase, subsequently these cells express rpr and die. Ectopic E2f expression during the S phase in normally cycling cells blocks their re-entry into S phase in the following cell cycle. These results show that an elevation in the level of E2f is sufficient to induce imaginal disc cells to enter S phase. Also they suggest that the down regulation of E2f upon entry into S phase may be essential to prevent the induction of apoptosis.

      E2f is required at multiple stages of development and may have an important function in post-mitotic cells in addition to its role during cell proliferation.

      Ectopic expression of E2f together with Dp induces cell proliferation and cell death in the eye.

      E2f transcription factor-induced S phase requires CycE.

      Deduced protein sequence of transcripts from the E(var)93E locus identifies the E2f gene.

      Mutations of E2f dominantly enhance position effect variegation, as tested with w and y variegating rearrangements. Overexpression suppresses position-effect variegation.

      During G1 of cycle 17 in the endocycling cells, E2f activation is independent of CycE, whereas CycE expression requires E2f. In the CNS CycE is expressed by a route independent of E2f, and the activation of E2f depends on CycE. The hierarchical relationship of CycE and E2f is reversed by tissue-specific distinctions in the mode of expression of CycE.

      E2f is essential in most cells for a G1-S transcriptional program and for G1-S progression.

      Analysis of expression patterns of E2f indicates in late stage embryos the protein is present in a restricted group of neural cells. In early embryos the protein is widely expressed in a segment restricted pattern. Distinct expression patterns of Dp and E2f suggest that the formation of Dp/E2f heterodimers is subject to complex regulatory cues.

      A dominant modifier of position effect variegation.

      Dp and E2f gene products interact and cooperate to give sequence specific DNA binding and optimal trans-activation. This suggests that E2f may be composed of heterodimers and may be regulated by retinoblastoma proteins.

      The product of E2f binds to an E2F recognition site and can activate transcription of Ecol\CAT reporter plasmids in Drosophila cells. The transcriptional activation function can be found in C-terminal sequences. A potential gene target for E2f is DNA-polymerase α.

      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 93 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      EMBL-EBI Single Cell Expression Atlas
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (30)
      Reported As
      Symbol Synonym
      DRTF1/E2F
      E(Sev-CycE)3A
      E(var)93E
      E2F
      (Li and Hidalgo, 2020, Sun et al., 2019, Tokusumi et al., 2015, Bhaskar et al., 2014, Kwon et al., 2013, Losick et al., 2013, Adamson and Lajeunesse, 2012, Ge et al., 2012, Korenjak et al., 2012, Valadez-Graham et al., 2012, Wang et al., 2012, Chen et al., 2011, Slattery et al., 2011, Turner et al., 2011, Walker et al., 2011, Buttitta et al., 2010, Herranz et al., 2010, Lee et al., 2010, Glavic et al., 2009, Lyulcheva et al., 2008, Mitchell et al., 2008, Oktaba et al., 2008, Frolov et al., 2007, Sandmann et al., 2007, Buttitta and Edgar, 2006, Mukherjee et al., 2006, Parrish et al., 2006, Umetsu et al., 2006, Alvarez-Garcia and Miska, 2005, Bandura, 2005, Kleino et al., 2005, Kohzaki and Murakami, 2005, Sundaram, 2005, Thomas, 2005, Kearney et al., 2004, Kupsco et al., 2004, Moon et al., 2004, Tower, 2004, Volkova et al., 2004, Bergmann and Lane, 2003, Davidson, 2003, Heriche et al., 2003, Kramer et al., 2003, Ryoo and Steller, 2003, Thacker et al., 2003, Folberg-Blum et al., 2002, Frolov et al., 2001, Hirose et al., 2001, Potter and Xu, 2001, Datar et al., 2000, Li and Vaessin, 2000, Manak et al., 2000, Oldham et al., 2000, Asano and Wharton, 1999, Datar and Edgar, 1999, Enikolopov et al., 1999, Galloni and Edgar, 1999, Hayashi and Yamaguchi, 1999, Hinds, 1999, Whittaker et al., 1999, Britton and Edgar, 1998, Caldwell and Datta, 1998, Follette et al., 1998, Sasaki, 1998.3.3, Sass and Henikoff, 1998, Su and O'Farrell, 1998, Wallrath, 1998, Royzman et al., 1997, Royzman et al., 1997, Yamaguchi et al., 1997, Asano et al., 1996, Lin et al., 1996, Dynlacht et al., 1994, Dyson, 1994.6.6)
      E2F transcription factor 1
      Evar(3)164
      l(3)07172
      l(3)j3B1
      l(3)j3C2
      l(3)rM729
      Name Synonyms
      E2-promoter binding factor
      E2F transcription factor
      E2F transcription factor 1
      Secondary FlyBase IDs
      • FBgn0010918
      • FBgn0011343
      • FBgn0011351
      • FBgn0011520
      • FBgn0011767
      • FBgn0013944
      • FBgn0016652
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (508)