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Gene Dmel\E2f

General Information
Symbol	Dmel\E2f	Species	D. melanogaster
Name	E2F transcription factor	Annotation symbol	CG6376
Feature type	protein_coding_gene	FlyBase ID	FBgn0011766
Gene Model Status	Current	Stock availability	37 publicly available
Also Known As	dE2f1, dE2F, E2f1, E(var)3-93E, E2F-1, drosE2F1
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	93E9-93F1	Sequence location	3R:17,446,026..17,486,161 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene E2F transcription factor is referred to in FlyBase by the symbol Dmel\E2f (CG6376, FBgn0011766). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding transcription factor activity; protein binding; transcription factor binding; sequence-specific DNA binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; DNA binding. There is experimental evidence for 13 unique biological process terms, many of which group under: biological regulation; single-organism developmental process; neuron differentiation; positive regulation of biological process; immune response; cellular component organization or biogenesis; post-embryonic organ morphogenesis; cell cycle; muscle tissue development; immune system process; cell cycle process; negative regulation of cellular biosynthetic process; positive regulation of cellular biosynthetic process; regulation of developmental process; regulation of apoptotic process; cellular process. 91 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; adult segment; imaginal precursor; external compound sense organ; portion of tissue; cell part; thoracic segment; endoderm derivative; ventral nerve cord primordium. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: E2F Family; Transcription factor E2F/dimerisation partner (TDP); Winged helix-turn-helix transcription repressor DNA-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, larval salivary gland, larval trachea, adult spermathecae, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult ovary, adult male accessory gland, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1625387_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of E2f. Gene sequence location is 3R:17446026..17486161.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Alleles E2f[dsRNA.Scer\UAS.cJa] E2f[MI07609] Transgenic Constructs P{UAS-E2f.dsRNA.J}
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 93E9-93F1   Limits computationally determined from genome sequence between P{PZ}InR05545 and P{PZ}tsl00617
Experimentally Determined Cytological Location
Cytogenetic map Notes References 93E8-93E9   (determined by in situ hybridisation)   (Spradling et al., 1999) 93E-93E   (determined by in situ hybridisation)   (Seum et al., 1996) 93E-93E   (determined by in situ hybridisation)   (Duronio et al., 1995) 93E-93E   (determined by in situ hybridisation)   (Spierer et al., 1995) 86C3-86C4     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\E2f for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47 Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) E2f-RA FBtr0084119  NM_079713   3913   805 E2f-RB FBtr0084117  NM_169961   3157   805 E2f-RC FBtr0084118  NM_169962   4395   805 E2f-RD FBtr0334678  NM_001275880   5025   821
Additional Transcript Data & Comments
Reported size (kB)	4.7 (northern blot) (Sawado et al., 1998) 4.5 (longest cDNA) (Dynlacht et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein E2f-PA   FBpp0083518   87.5   805   6.64     NP_524437   AAF55904 E2f-PB   FBpp0083516   87.5   805   6.64     NP_732646   AAN13878 E2f-PC   FBpp0083517   87.5   805   6.64     NP_732647   AAN13879 E2f-PD   FBpp0306739   89.3   821   6.72     NP_001262809   AGB96189
Additional Polypeptide Data & Comments
Reported size (kDa)	805 (aa); 88 (kD predicted) (Ohtani and Nevins, 1994) 805 (aa) (Dynlacht et al., 1994)
Comments	E2f2 protein requires Dp protein for binding to the E2f recognition sites of the adenovirus E2 promoter. E2f protein does not require Dp protein for binding the same site, but binding is enhanced in the presence of Dp protein. E2f2 binds to one of two E2f recognition sites in the mus209 gene promoter. Unlike the transcription activator E2f, E2f2 acts as a repressor of expression from the mus209 promoter. (Sawado et al., 1998) Cotransfection assays in S2 cells were used to demonstrate that E2f protein and Dp protein form a heteromeric complex and cooperate to give sequence-specific DNA binding and transcriptional activation. Deletion mutants of E2f protein were made to identify a region of E2f protein necessary for interaction with Dp protein. (Dynlacht et al., 1994) E2f protein expressed in bacteria was shown to have DNA-binding activity and to bind specifically to E2f recognition sequences. In S2 cells, E2f protein stimulates transcription and the activation is dependent on functional E2f binding sites. The transcription activation function was mapped to the C-terminal portion of the E2f protein between amino acids 560 and 769. Finally, E2f protein recognition sequences were found within the promoter of the DNA polymerase alpha gene. (Ohtani and Nevins, 1994)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor E2F/dimerisation partner (TDP) (IPR003316) Winged helix-turn-helix DNA-binding domain (IPR011991) E2F Family (IPR015633)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA142142   AA263417   AA540210   AA540788   AA695506   AA697620   AA735447   AA802049   AA820506   AA940847   AA940911   AA978498   AE014297 AAF55904   AE014297 AAN13878   AE014297 AAN13879   AB011813 BAA32746   AE014297 AGB96189   AI063281   AI260501   AI296470   AI386589   AI388391   AI388869   AI456281   AI456699   AI513441   AI516168   AI519414   AI520292   AI520320   AI546122   AW940634   AY069149 AAL39294   BF495690   BF497039   BG638401   BG638791   BG639026   BI163140   BI213830   BI235274   BI239059   BI356406   BI633999   BI636488   BP558144   BP558145   BQ103633   BT016096 AAV36981   CK132858   CK135917   CL528112   CL610161   CL943631   CO187488   CO265552   CO267037   CO267075   CO270274   CO270485   CO271708   CO281736   CO283005   CO283074   CO287869   CO288192   CO294432   CO295065   CO298434   CO302330   CO302380   CO309279   CO310983   CO311741   CO317726   CO319226   CO325184   CO325591   CO326456   CO326876   CO326899   CO329933   CO333686   CO333709   CO336822   CO337804   CO340719   CO340872   CO341927   CO343620   CZ470466   CZ472277   CZ472982   CZ473028   CZ473672   CZ474760   CZ474761   CZ475036   CZ484015   CZ484485   CZ487849   CZ488593   CZ489675   DN154262   DN154501   DU708632   EC053493   EC058650   EC061442   EC074891   EC079410   EC081080   EC083134   EC084290   EC085077   EC085513   EC086898   EC089960   EC202169   EC205900   EC210318   EC213672   EC217101   EC218926   EC222726   EC225055   EC225323   EC231407   EC233439   EC234640   EC242493   EC242798   EC243506   EC249984   EC251876   EC255877   EC257376   EC259802   EC262743   EC263421   EC266035   EL885433   EV575617   EV578355   EV582386   EV583090   EV586167   S82201   S82217   U10184 AAA19003   X78421 CAA55186
UniProtKB/Swiss-Prot	Q27368
UniProtKB/TrEMBL	Q8T0P0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References protein binding site FBsf0000159743 3R:17,460,089..17,460,094 bound_moiety=cad-XP comment=cad-binding site is required for E2f expression in the posterior midgut and hindgut. evidence=experimental (Hwang et al., 2002) protein binding site FBsf0000159744 3R:17,459,867..17,459,873 bound_moiety=cad-XP comment=cad-binding site is required for E2f expression in the posterior midgut and hindgut. evidence=experimental (Hwang et al., 2002) protein binding site FBsf0000159745 3R:17,459,780..17,459,785 evidence=experimental bound_moiety=cad-XP comment=cad-binding site is required for E2f expression in the posterior midgut and hindgut. (Hwang et al., 2002) protein binding site FBsf0000159815 3R:17,458,956..17,458,971 bound_moiety=Dref-XP evidence=experimental (Sawado et al., 1998, Bergman et al., 2005) protein binding site FBsf0000159816 3R:17,459,105..17,459,140 evidence=experimental (Bergman et al., 2005, Sawado et al., 1998) protein binding site FBsf0000160884 3R:17,458,940..17,458,977 evidence=experimental (Sawado et al., 1998, Bergman et al., 2005) protein binding site FBsf0000160885 3R:17,458,945..17,458,952 bound_moiety=Dref-XP evidence=experimental (Sawado et al., 1998, Bergman et al., 2005) protein binding site FBsf0000160886 3R:17,459,045..17,459,066 evidence=experimental (Bergman et al., 2005, Sawado et al., 1998) regulatory region FBsf0000435543 3R:17,458,945..17,458,971 evidence=Reporter construct (in vitro) (Sawado et al., 1998, Halfon, 2012.6.19) regulatory region FBsf0000436614 3R:17,458,229..17,458,871 evidence=Reporter construct (in vitro) (Sawado et al., 1998, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early     (Hao et al., 1995) embryonic stage   presumptive embryonic/larval central nervous system   (Duronio et al., 1995) neuroblast   (Duronio et al., 1995) embryonic stage 1   organism | ubiquitous   (Seum et al., 1996) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 1 -- 4     (Duronio et al., 1995) embryonic stage 4 -- 6   anlage in statu nascendi   (Fisher et al., 2012) central brain anlage in statu nascendi   (Fisher et al., 2012) ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi organism | gap expression pattern   (Fisher et al., 2012) antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi embryonic stage 7   ventral furrow   (Seum et al., 1996) embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal ectoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 8 -- 11   mesoderm   (Seum et al., 1996) embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 9 -- 11   neuroblast   (Seum et al., 1996) embryonic stage 10 -- 17   organism | ubiquitous   (Duronio et al., 1995) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic central brain surface glia   (Fisher et al., 2012) embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) lateral cord surface glial cell   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) procephalic neuroblast   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 12   presumptive embryonic/larval central nervous system | restricted   (Hao et al., 1995) embryonic stage 12 -- 17   embryonic brain   (Hao et al., 1995) embryonic stage 13   anterior embryonic/larval midgut   (Seum et al., 1996) posterior embryonic/larval midgut   (Seum et al., 1996) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic midgut chamber   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval midgut interstitial cell   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) gastric caecum   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) embryonic stage 16   embryonic/larval midgut   (Seum et al., 1996) embryonic/larval foregut   (Seum et al., 1996) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Sawado et al., 1998)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Ohtani and Nevins, 1994) Rb-E2F complex inferred from direct assay (Du et al., 1996, Stevaux et al., 2002)
Expression Deduced from Reporters
Reporter: P{PZ}E2frM729 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval central nervous system   (Duronio et al., 1995) embryonic stage 10 -- 17   organism | ubiquitous   (Duronio et al., 1995)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, larval salivary gland, larval trachea, adult spermathecae, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult ovary, adult male accessory gland, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1317.275 Larval Midgut    365.2 Larval Hindgut    619.8 Larval Malpighian Tubules    360.7 Larval Fat Body    183.5 Larval Salivary Gland    966.1 Larval Trachea    782.875 Larval Carcass    597.05 Adult Head    207 Adult Eye    282.925 Adult Brain    339.9 Adult Thoracic-Abdominal Ganglion    487 Adult Crop    408.4 Adult Midgut    223 Adult Hindgut    326.3 Adult Malpighian Tubules    218 Adult Fat Body    246.8 Adult Salivary Gland    351.5 Adult Heart    277.325 Adult VirginFemale Spermatheca    673.6 Adult InseminatedFemale Spermatheca    713.9 Adult Ovary    156.6 Adult Testis    35.2 Adult Male Accessory Gland    470.3 Adult Carcass    158.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1317.275) Larval Midgut    365.2 Larval Hindgut  (619.8) Larval Malpighian Tubules    360.7 Larval Fat Body    183.5 Larval Salivary Gland  (966.1) Larval Trachea  (782.875) Larval Carcass  (597.05) Adult Head    207 Adult Eye    282.925 Adult Brain    339.9 Adult Thoracic-Abdominal Ganglion    487 Adult Crop    408.4 Adult Midgut    223 Adult Hindgut    326.3 Adult Malpighian Tubules    218 Adult Fat Body    246.8 Adult Salivary Gland    351.5 Adult Heart    277.325 Adult VirginFemale Spermatheca  (673.6) Adult InseminatedFemale Spermatheca  (713.9) Adult Ovary    156.6 Adult Testis    35.2 Adult Male Accessory Gland    470.3 Adult Carcass    158.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1317.275) Larval Midgut    365.2 Larval Hindgut    619.8 Larval Malpighian Tubules    360.7 Larval Fat Body    183.5 Larval Salivary Gland    966.1 Larval Trachea    782.875 Larval Carcass    597.05 Adult Head    207 Adult Eye    282.925 Adult Brain    339.9 Adult Thoracic-Abdominal Ganglion    487 Adult Crop    408.4 Adult Midgut    223 Adult Hindgut    326.3 Adult Malpighian Tubules    218 Adult Fat Body    246.8 Adult Salivary Gland    351.5 Adult Heart    277.325 Adult VirginFemale Spermatheca    673.6 Adult InseminatedFemale Spermatheca    713.9 Adult Ovary    156.6 Adult Testis    35.2 Adult Male Accessory Gland    470.3 Adult Carcass    158.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1317.275 Larval Midgut    365.2 Larval Hindgut    619.8 Larval Malpighian Tubules    360.7 Larval Fat Body    183.5 Larval Salivary Gland    966.1 Larval Trachea    782.875 Larval Carcass    597.05 Adult Head    207 Adult Eye    282.925 Adult Brain    339.9 Adult Thoracic-Abdominal Ganglion    487 Adult Crop    408.4 Adult Midgut    223 Adult Hindgut    326.3 Adult Malpighian Tubules    218 Adult Fat Body    246.8 Adult Salivary Gland    351.5 Adult Heart    277.325 Adult VirginFemale Spermatheca    673.6 Adult InseminatedFemale Spermatheca    713.9 Adult Ovary    156.6 Adult Testis    35.2 Adult Male Accessory Gland    470.3 Adult Carcass    158.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1317.275 Larval Midgut    365.2 Larval Hindgut    619.8 Larval Malpighian Tubules    360.7 Larval Fat Body    183.5 Larval Salivary Gland    966.1 Larval Trachea    782.875 Larval Carcass    597.05 Adult Head    207 Adult Eye    282.925 Adult Brain    339.9 Adult Thoracic-Abdominal Ganglion    487 Adult Crop    408.4 Adult Midgut    223 Adult Hindgut    326.3 Adult Malpighian Tubules    218 Adult Fat Body    246.8 Adult Salivary Gland    351.5 Adult Heart    277.325 Adult VirginFemale Spermatheca    673.6 Adult InseminatedFemale Spermatheca    713.9 Adult Ovary    156.6 Adult Testis    35.2 Adult Male Accessory Gland    470.3 Adult Carcass    158.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1317.275) Larval Midgut    365.2 Larval Hindgut  619.8 Larval Malpighian Tubules    360.7 Larval Fat Body    183.5 Larval Salivary Gland  (966.1) Larval Trachea  782.875 Larval Carcass  597.05 Adult Head    207 Adult Eye    282.925 Adult Brain    339.9 Adult Thoracic-Abdominal Ganglion    487 Adult Crop    408.4 Adult Midgut    223 Adult Hindgut    326.3 Adult Malpighian Tubules    218 Adult Fat Body    246.8 Adult Salivary Gland    351.5 Adult Heart    277.325 Adult VirginFemale Spermatheca  673.6 Adult InseminatedFemale Spermatheca  713.9 Adult Ovary    156.6 Adult Testis    35.2 Adult Male Accessory Gland    470.3 Adult Carcass    158.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1317.275 Larval Midgut    365.2 Larval Hindgut    619.8 Larval Malpighian Tubules    360.7 Larval Fat Body    183.5 Larval Salivary Gland    966.1 Larval Trachea    782.875 Larval Carcass    597.05 Adult Head    207 Adult Eye    282.925 Adult Brain    339.9 Adult Thoracic-Abdominal Ganglion    487 Adult Crop    408.4 Adult Midgut    223 Adult Hindgut    326.3 Adult Malpighian Tubules    218 Adult Fat Body    246.8 Adult Salivary Gland    351.5 Adult Heart    277.325 Adult VirginFemale Spermatheca    673.6 Adult InseminatedFemale Spermatheca    713.9 Adult Ovary    156.6 Adult Testis    35.2 Adult Male Accessory Gland    470.3 Adult Carcass    158.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1317.275 Larval Midgut    365.2 Larval Hindgut    619.8 Larval Malpighian Tubules    360.7 Larval Fat Body    183.5 Larval Salivary Gland    966.1 Larval Trachea    782.875 Larval Carcass    597.05 Adult Head    207 Adult Eye    282.925 Adult Brain    339.9 Adult Thoracic-Abdominal Ganglion    487 Adult Crop    408.4 Adult Midgut    223 Adult Hindgut    326.3 Adult Malpighian Tubules    218 Adult Fat Body    246.8 Adult Salivary Gland    351.5 Adult Heart    277.325 Adult VirginFemale Spermatheca    673.6 Adult InseminatedFemale Spermatheca    713.9 Adult Ovary    156.6 Adult Testis    35.2 Adult Male Accessory Gland    470.3 Adult Carcass    158.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    207   NA  Eye    282.925   NA  Brain    339.9   1317.275  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    487   NA  Crop    408.4   365.2  Midgut    223   619.8  Hindgut    326.3   360.7  Malpighian Tubules    218   183.5  Fat Body    246.8   966.1  Salivary Gland    351.5   NA  Heart    277.325   782.875  Trachea    NA   NA  VirginFemale Spermatheca    673.6   NA  InseminatedFemale Spermatheca    713.9   NA  Ovary    156.6   NA  Testis    35.2   NA  Male Accessory Gland    470.3   597.05  Carcass    158.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    110 central nervous system, pupae P8    41 head, virgin 1-day female    15 head, virgin 4-day female    16 head, virgin 20-day female    10 head, mated 1-day female    20 head, mated 4-day female    18 head, mated 20-day female    13 head, mated 1-day male    22 head, mated 4-day male    27 head, mated 20-day male    26 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    16 digestive system, 4-day adult    25 digestive system, 20-day adult    18 fat body, larvae L3 wandering    12 fat body, white prepupae    19 fat body, pupae P8    21 carcass, larvae L3 wandering    47 carcass, 1-day adult    15 carcass, 4-day adult    22 carcass, 20-day adult    20 ovary, virgin 4-day female    78 ovary, mated 4-day female    91 testis, mated 4-day male    8 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (38) central nervous system, larvae L3  (110) central nervous system, pupae P8  (41) head, virgin 1-day female    15 head, virgin 4-day female    16 head, virgin 20-day female    10 head, mated 1-day female    20 head, mated 4-day female    18 head, mated 20-day female    13 head, mated 1-day male    22 head, mated 4-day male  (27) head, mated 20-day male  26 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    14 digestive system, larvae L3 wandering  (29) digestive system, 1-day adult    16 digestive system, 4-day adult    25 digestive system, 20-day adult    18 fat body, larvae L3 wandering    12 fat body, white prepupae    19 fat body, pupae P8    21 carcass, larvae L3 wandering  (47) carcass, 1-day adult    15 carcass, 4-day adult    22 carcass, 20-day adult    20 ovary, virgin 4-day female  (78) ovary, mated 4-day female  (91) testis, mated 4-day male    8 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3  (110) central nervous system, pupae P8    41 head, virgin 1-day female    15 head, virgin 4-day female    16 head, virgin 20-day female    10 head, mated 1-day female    20 head, mated 4-day female    18 head, mated 20-day female    13 head, mated 1-day male    22 head, mated 4-day male    27 head, mated 20-day male    26 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    16 digestive system, 4-day adult    25 digestive system, 20-day adult    18 fat body, larvae L3 wandering    12 fat body, white prepupae    19 fat body, pupae P8    21 carcass, larvae L3 wandering    47 carcass, 1-day adult    15 carcass, 4-day adult    22 carcass, 20-day adult    20 ovary, virgin 4-day female    78 ovary, mated 4-day female    91 testis, mated 4-day male    8 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    110 central nervous system, pupae P8    41 head, virgin 1-day female    15 head, virgin 4-day female    16 head, virgin 20-day female    10 head, mated 1-day female    20 head, mated 4-day female    18 head, mated 20-day female    13 head, mated 1-day male    22 head, mated 4-day male    27 head, mated 20-day male    26 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    16 digestive system, 4-day adult    25 digestive system, 20-day adult    18 fat body, larvae L3 wandering    12 fat body, white prepupae    19 fat body, pupae P8    21 carcass, larvae L3 wandering    47 carcass, 1-day adult    15 carcass, 4-day adult    22 carcass, 20-day adult    20 ovary, virgin 4-day female    78 ovary, mated 4-day female    91 testis, mated 4-day male    8 accessory gland, mated 4-day male    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    110 central nervous system, pupae P8    41 head, virgin 1-day female    15 head, virgin 4-day female    16 head, virgin 20-day female    10 head, mated 1-day female    20 head, mated 4-day female    18 head, mated 20-day female    13 head, mated 1-day male    22 head, mated 4-day male    27 head, mated 20-day male    26 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    16 digestive system, 4-day adult    25 digestive system, 20-day adult    18 fat body, larvae L3 wandering    12 fat body, white prepupae    19 fat body, pupae P8    21 carcass, larvae L3 wandering    47 carcass, 1-day adult    15 carcass, 4-day adult    22 carcass, 20-day adult    20 ovary, virgin 4-day female    78 ovary, mated 4-day female    91 testis, mated 4-day male    8 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (38) central nervous system, larvae L3  (110) central nervous system, pupae P8  (41) head, virgin 1-day female    15 head, virgin 4-day female    16 head, virgin 20-day female    10 head, mated 1-day female    20 head, mated 4-day female    18 head, mated 20-day female    13 head, mated 1-day male    22 head, mated 4-day male  27 head, mated 20-day male  26 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    14 digestive system, larvae L3 wandering  29 digestive system, 1-day adult    16 digestive system, 4-day adult    25 digestive system, 20-day adult    18 fat body, larvae L3 wandering    12 fat body, white prepupae    19 fat body, pupae P8    21 carcass, larvae L3 wandering  (47) carcass, 1-day adult    15 carcass, 4-day adult    22 carcass, 20-day adult    20 ovary, virgin 4-day female  (78) ovary, mated 4-day female  (91) testis, mated 4-day male    8 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3  110 central nervous system, pupae P8    41 head, virgin 1-day female    15 head, virgin 4-day female    16 head, virgin 20-day female    10 head, mated 1-day female    20 head, mated 4-day female    18 head, mated 20-day female    13 head, mated 1-day male    22 head, mated 4-day male    27 head, mated 20-day male    26 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    16 digestive system, 4-day adult    25 digestive system, 20-day adult    18 fat body, larvae L3 wandering    12 fat body, white prepupae    19 fat body, pupae P8    21 carcass, larvae L3 wandering    47 carcass, 1-day adult    15 carcass, 4-day adult    22 carcass, 20-day adult    20 ovary, virgin 4-day female    78 ovary, mated 4-day female    91 testis, mated 4-day male    8 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    110 central nervous system, pupae P8    41 head, virgin 1-day female    15 head, virgin 4-day female    16 head, virgin 20-day female    10 head, mated 1-day female    20 head, mated 4-day female    18 head, mated 20-day female    13 head, mated 1-day male    22 head, mated 4-day male    27 head, mated 20-day male    26 salivary gland, larvae L3 wandering    10 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    16 digestive system, 4-day adult    25 digestive system, 20-day adult    18 fat body, larvae L3 wandering    12 fat body, white prepupae    19 fat body, pupae P8    21 carcass, larvae L3 wandering    47 carcass, 1-day adult    15 carcass, 4-day adult    22 carcass, 20-day adult    20 ovary, virgin 4-day female    78 ovary, mated 4-day female    91 testis, mated 4-day male    8 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    42 embryo 04-06hr    51 embryo 06-08hr    71 embryo 08-10hr    49 embryo 10-12hr    40 embryo 12-14hr    28 embryo 14-16hr    27 embryo 16-18hr    20 embryo 18-20hr    18 embryo 20-22hr    15 embryo 22-24hr    13 larva L1    17 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    22 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    27 adult female 05day    36 adult female 30day    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (56) embryo 02-04hr  (42) embryo 04-06hr  (51) embryo 06-08hr  (71) embryo 08-10hr  (49) embryo 10-12hr  (40) embryo 12-14hr  (28) embryo 14-16hr  (27) embryo 16-18hr    20 embryo 18-20hr    18 embryo 20-22hr    15 embryo 22-24hr    13 larva L1    17 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6  (27) larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    22 white prepupae 24hr  (31) pupae 2d postWPP    20 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day  (27) adult female 05day  (36) adult female 30day  (36) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    42 embryo 04-06hr    51 embryo 06-08hr    71 embryo 08-10hr    49 embryo 10-12hr    40 embryo 12-14hr    28 embryo 14-16hr    27 embryo 16-18hr    20 embryo 18-20hr    18 embryo 20-22hr    15 embryo 22-24hr    13 larva L1    17 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    22 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    27 adult female 05day    36 adult female 30day    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    42 embryo 04-06hr    51 embryo 06-08hr    71 embryo 08-10hr    49 embryo 10-12hr    40 embryo 12-14hr    28 embryo 14-16hr    27 embryo 16-18hr    20 embryo 18-20hr    18 embryo 20-22hr    15 embryo 22-24hr    13 larva L1    17 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    22 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    27 adult female 05day    36 adult female 30day    36 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    42 embryo 04-06hr    51 embryo 06-08hr    71 embryo 08-10hr    49 embryo 10-12hr    40 embryo 12-14hr    28 embryo 14-16hr    27 embryo 16-18hr    20 embryo 18-20hr    18 embryo 20-22hr    15 embryo 22-24hr    13 larva L1    17 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    22 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    27 adult female 05day    36 adult female 30day    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (56) embryo 02-04hr  (42) embryo 04-06hr  (51) embryo 06-08hr  (71) embryo 08-10hr  (49) embryo 10-12hr  (40) embryo 12-14hr  28 embryo 14-16hr  27 embryo 16-18hr    20 embryo 18-20hr    18 embryo 20-22hr    15 embryo 22-24hr    13 larva L1    17 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6  27 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    22 white prepupae 24hr  31 pupae 2d postWPP    20 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day  27 adult female 05day  (36) adult female 30day  (36) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    42 embryo 04-06hr    51 embryo 06-08hr    71 embryo 08-10hr    49 embryo 10-12hr    40 embryo 12-14hr    28 embryo 14-16hr    27 embryo 16-18hr    20 embryo 18-20hr    18 embryo 20-22hr    15 embryo 22-24hr    13 larva L1    17 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    22 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    27 adult female 05day    36 adult female 30day    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    56 embryo 02-04hr    42 embryo 04-06hr    51 embryo 06-08hr    71 embryo 08-10hr    49 embryo 10-12hr    40 embryo 12-14hr    28 embryo 14-16hr    27 embryo 16-18hr    20 embryo 18-20hr    18 embryo 20-22hr    15 embryo 22-24hr    13 larva L1    17 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    14 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    22 white prepupae new    20 white prepupae 12hr    22 white prepupae 24hr    31 pupae 2d postWPP    20 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    27 adult female 05day    36 adult female 30day    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    98 Schneider line 2 Sg4    40 embryonic 1182-4H    79 embryonic GM2    61 embryonic Kc167    56 embryonic S1    31 embryonic S3    48 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    60 wing disc ML-DmD9    52 wing disc ML-DmD16-c3    58 wing disc ML-DmD21    45 wing disc ML-DmD32    120 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    46 CNS ML-DmBG2-c2    35 tumorous blood cells mbn2    31 ovary fGS/OSS    63 ovary OSC    102 ovary OSS    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (98) Schneider line 2 Sg4  (40) embryonic 1182-4H  (79) embryonic GM2  (61) embryonic Kc167  (56) embryonic S1  (31) embryonic S3  (48) leg disc CME L1  (40) wing disc CME-W2  (34) wing disc ML-DmD8  (60) wing disc ML-DmD9  (52) wing disc ML-DmD16-c3  (58) wing disc ML-DmD21  (45) wing disc ML-DmD32  (120) haltere disc ML-DmD17-c3  (28) eye-antennal disc ML-DmD11  (28) antennal disc ML-DmD20-c5  (27) mixed discs ML-DmD4-c1  (45) CNS ML-DmBG1-c1  (46) CNS ML-DmBG2-c2  (35) tumorous blood cells mbn2  (31) ovary fGS/OSS  (63) ovary OSC  (102) ovary OSS  (58) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    98 Schneider line 2 Sg4    40 embryonic 1182-4H    79 embryonic GM2    61 embryonic Kc167    56 embryonic S1    31 embryonic S3    48 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    60 wing disc ML-DmD9    52 wing disc ML-DmD16-c3    58 wing disc ML-DmD21    45 wing disc ML-DmD32  (120) haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    46 CNS ML-DmBG2-c2    35 tumorous blood cells mbn2    31 ovary fGS/OSS    63 ovary OSC  102 ovary OSS    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    98 Schneider line 2 Sg4    40 embryonic 1182-4H    79 embryonic GM2    61 embryonic Kc167    56 embryonic S1    31 embryonic S3    48 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    60 wing disc ML-DmD9    52 wing disc ML-DmD16-c3    58 wing disc ML-DmD21    45 wing disc ML-DmD32    120 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    46 CNS ML-DmBG2-c2    35 tumorous blood cells mbn2    31 ovary fGS/OSS    63 ovary OSC    102 ovary OSS    58 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    98 Schneider line 2 Sg4    40 embryonic 1182-4H    79 embryonic GM2    61 embryonic Kc167    56 embryonic S1    31 embryonic S3    48 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    60 wing disc ML-DmD9    52 wing disc ML-DmD16-c3    58 wing disc ML-DmD21    45 wing disc ML-DmD32    120 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    46 CNS ML-DmBG2-c2    35 tumorous blood cells mbn2    31 ovary fGS/OSS    63 ovary OSC    102 ovary OSS    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (98) Schneider line 2 Sg4  (40) embryonic 1182-4H  (79) embryonic GM2  (61) embryonic Kc167  (56) embryonic S1  31 embryonic S3  (48) leg disc CME L1  (40) wing disc CME-W2  (34) wing disc ML-DmD8  (60) wing disc ML-DmD9  (52) wing disc ML-DmD16-c3  (58) wing disc ML-DmD21  (45) wing disc ML-DmD32  (120) haltere disc ML-DmD17-c3  28 eye-antennal disc ML-DmD11  28 antennal disc ML-DmD20-c5  27 mixed discs ML-DmD4-c1  (45) CNS ML-DmBG1-c1  (46) CNS ML-DmBG2-c2  (35) tumorous blood cells mbn2  31 ovary fGS/OSS  (63) ovary OSC  (102) ovary OSS  (58) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    98 Schneider line 2 Sg4    40 embryonic 1182-4H    79 embryonic GM2    61 embryonic Kc167    56 embryonic S1    31 embryonic S3    48 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    60 wing disc ML-DmD9    52 wing disc ML-DmD16-c3    58 wing disc ML-DmD21    45 wing disc ML-DmD32  120 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    46 CNS ML-DmBG2-c2    35 tumorous blood cells mbn2    31 ovary fGS/OSS    63 ovary OSC  102 ovary OSS    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    98 Schneider line 2 Sg4    40 embryonic 1182-4H    79 embryonic GM2    61 embryonic Kc167    56 embryonic S1    31 embryonic S3    48 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    60 wing disc ML-DmD9    52 wing disc ML-DmD16-c3    58 wing disc ML-DmD21    45 wing disc ML-DmD32    120 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    45 CNS ML-DmBG1-c1    46 CNS ML-DmBG2-c2    35 tumorous blood cells mbn2    31 ovary fGS/OSS    63 ovary OSC    102 ovary OSS    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    39 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    31 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    43 Copper 15 mM 48 hrs, 4-day adult    65 Zinc 5 mM 12 hrs, larvae L3    42 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (48) cold shock, 4-day adult  (39) heat shock, 4-day adult  (29) Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult  (31) Cadmium 100 mM 48 hrs, 4-day adult  (54) Copper 0.5 mM 12 hrs, larvae L3  (43) Copper 15 mM 48 hrs, 4-day adult  (65) Zinc 5 mM 12 hrs, larvae L3  (42) Zinc 4.5 mM 48 hrs, 4-day adult  (35) Ethanol 2.5% 3 hrs, larvae L3  (46) Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (48) Caffeine 25 mg/ml 48 hrs, 4-day adult  (39) Paraquat 5 mM 48 hrs, 4-day adult  (44) Paraquat 10 mM 48 hrs, 4-day adult  (35) Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    39 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    31 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    43 Copper 15 mM 48 hrs, 4-day adult    65 Zinc 5 mM 12 hrs, larvae L3    42 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    39 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    31 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    43 Copper 15 mM 48 hrs, 4-day adult    65 Zinc 5 mM 12 hrs, larvae L3    42 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    39 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    31 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    43 Copper 15 mM 48 hrs, 4-day adult    65 Zinc 5 mM 12 hrs, larvae L3    42 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (48) cold shock, 4-day adult  (39) heat shock, 4-day adult  29 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult  31 Cadmium 100 mM 48 hrs, 4-day adult  (54) Copper 0.5 mM 12 hrs, larvae L3  (43) Copper 15 mM 48 hrs, 4-day adult  (65) Zinc 5 mM 12 hrs, larvae L3  (42) Zinc 4.5 mM 48 hrs, 4-day adult  (35) Ethanol 2.5% 3 hrs, larvae L3  (46) Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (48) Caffeine 25 mg/ml 48 hrs, 4-day adult  (39) Paraquat 5 mM 48 hrs, 4-day adult  (44) Paraquat 10 mM 48 hrs, 4-day adult  (35) Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    39 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    31 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    43 Copper 15 mM 48 hrs, 4-day adult    65 Zinc 5 mM 12 hrs, larvae L3    42 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    39 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    31 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    43 Copper 15 mM 48 hrs, 4-day adult    65 Zinc 5 mM 12 hrs, larvae L3    42 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    15 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • GH16721 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011766 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6376-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for E2f Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal E2fScer\UAS.cNa lethal, with Scer\GAL430A E2fScer\UAS.cNa lethal, with Scer\GAL432B E2fScer\UAS.cNa lethal, with Scer\GAL434B E2fScer\UAS.cNa lethal, with Scer\GAL471B E2fScer\UAS.cNa lethal, with Scer\GAL4179Y E2fScer\UAS.cNa lethal, with Scer\GAL41854 E2fScer\UAS.cNa lethal, with Scer\GAL43735 E2fScer\UAS.cNa lethal, with Scer\GAL44935 E2fScer\UAS.cNa lethal, with Scer\GAL4Act5C.PI E2fScer\UAS.cNa lethal, with Scer\GAL4ap-md544 E2fScer\UAS.cNa lethal, with Scer\GAL4arm.PS E2fScer\UAS.cNa lethal, with Scer\GAL4C23 E2fScer\UAS.cNa lethal, with Scer\GAL4c323a E2fScer\UAS.cNa lethal, with Scer\GAL4Cy6 E2fScer\UAS.cNa lethal, with Scer\GAL4da.G32 E2fScer\UAS.cNa lethal, with Scer\GAL4Dll-md23 E2fScer\UAS.cNa lethal, with Scer\GAL4dpp.blk1 E2fScer\UAS.cNa lethal, with Scer\GAL4ey.PH E2fScer\UAS.cNa lethal, with Scer\GAL4h-H10 E2fScer\UAS.cNa lethal, with Scer\GAL4how-24B E2fScer\UAS.cNa lethal, with Scer\GAL4sev.EP E2fScer\UAS.cNa lethal, with Scer\GAL4T80 E2fScer\UAS.cNa lethal, with Scer\GAL4tsh-md621 E2fScer\UAS.cNa lethal, with Scer\GAL4twi.2PE E2fScer\UAS.cNa lethal, with Scer\GAL4αTub84B.PL E2fScer\UAS.cNa lethal | larval stage (with E2f91) E2frM729 lethal | larval stage (with E2frM729) E2f91 lethal | recessive E2f91 E2f155 E2f296 E2f337 E2f07172 E2fE(Sev-CycE)3A E2fhs.PA E2fj3B1 E2fj3C2 E2fLA00365 E2frM729 partially lethal - majority die, with Scer\GAL4arm.PS E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP partially lethal - majority die, with Scer\GAL4en-e16E E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fScer\UAS.cNa partially lethal - majority die, with Scer\GAL4sca-T3 E2fScer\UAS.cNa partially lethal - majority die (with E2fmir-11-KO.w+) E2funspecified partially lethal - majority die (with E2funspecified) E2fmir-11-KO.w+ partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 E2fGD4448 viable E2f164 E2fEY05005 E2fEY08459 E2fEY16535 E2fi1 E2fi2 E2fsu89 viable (with E2f91) E2fi2 viable (with E2fi2) E2f91 viable (with E2fmir-11-R.w-) E2funspecified viable (with E2funspecified) E2fmir-11-R.w- Sterility Allele female fertile E2f164 female sterile E2fi1 E2fi2 female sterile, with E2f91 E2fhs.PD female sterile, with E2fhs.PD E2f91 female sterile | partially | recessive E2fi2 female sterile | recessive E2fi1 fertile E2fEY05005 E2fEY08459 E2fEY16535 E2fsu89 male fertile E2f164 Other Phenotypes Allele behavior defective E2f91 E2f07172 cell death defective E2fi2 cell death defective | third instar larval stage, with Scer\GAL4en-e16E E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A-Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP cell death defective | third instar larval stage, with Scer\GAL4GMR.PU E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A-Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP decreased cell death (with E2f07172) E2fi1 decreased cell death (with E2fi1) E2f07172 decreased cell size, with Scer\GAL4en-e16E E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP developmental rate defective (with E2f91) E2frM729 developmental rate defective (with E2frM729) E2f91 enhancer of variegation, with In(1)wm4 E2f91 enhancer of variegation | dominant, with Dp1187-8-23, In(1)wm4h E2f91 enhancer of variegation | dominant, with In(1)wm4h E2f164 increased cell death, with Scer\GAL4en-e16E E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP increased cell number, with Scer\GAL4sca-537.4 E2fScer\UAS.cNa increased cell size | somatic clone E2frM729 melanotic mass phenotype E2f91 E2f07172 mitotic cell cycle defective E2fdsRNA.cFa mitotic cell cycle defective, with Scer\GAL4en-e16E E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP mitotic cell cycle defective | third instar larval stage, with Scer\GAL4en-e16E E2fDBD.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A-Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fRb.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fScer\UAS.P\T.T:Avic\GFP-EGFP E2fTrunc.Scer\UAS.P\T.T:Avic\GFP-EGFP mitotic cell cycle defective | third instar larval stage, with Scer\GAL4GMR.PU E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP neuroanatomy defective E2f07172 neuroanatomy defective | homeotic, with Scer\GAL4sca-537.4 E2fScer\UAS.cNa non-enhancer of variegation | dominant, with P{hsp26-pt-T}39C-12 E2f164 non-enhancer of variegation | dominant, with P{hsp26-pt-T}39C-42 E2f164 non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-3 E2f164 non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-10 E2f164 non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-19 E2f164 non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-25-5 E2f164 non-enhancer of variegation | dominant, with P{hsp26-pt-T}CG231639C-34 E2f164 non-suppressor of variegation | dominant, with P{hsp26-pt-T}39C-12 E2f164 non-suppressor of variegation | dominant, with P{hsp26-pt-T}39C-42 E2f164 non-suppressor of variegation | dominant, with P{hsp26-pt-T}118E-3 E2f164 non-suppressor of variegation | dominant, with P{hsp26-pt-T}118E-10 E2f164 non-suppressor of variegation | dominant, with P{hsp26-pt-T}118E-19 E2f164 non-suppressor of variegation | dominant, with P{hsp26-pt-T}118E-25-5 E2f164 non-suppressor of variegation | dominant, with P{hsp26-pt-T}CG231639C-34 E2f164 radiation resistant | maternal effect E2funspecified suppressor of variegation | dominant, with In(1)wm4h E2fhs.PS visible E2fsu89 visible, with Scer\GAL4[-]: E2fdsRNA.C.Scer\UAS visible, with E2fdsRNA.Scer\UAS.cJa, Scer\GAL4GMR.PU E2fi1 visible, with E2fi1, Scer\GAL4GMR.PU E2fdsRNA.Scer\UAS.cJa visible, with Scer\GAL4Act88F.PD E2fScer\UAS.cNa visible, with Scer\GAL4en-e16E E2fScer\UAS.cNa visible, with Scer\GAL4GMR.PF E2fScer\UAS.cNa visible, with Scer\GAL4GMR.PU E2fdsRNA.Scer\UAS.cJa visible, with Scer\GAL4I-76-D E2fdsRNA.C.Scer\UAS visible, with Scer\GAL4nos.PG E2fScer\UAS.cNa visible, with Scer\GAL4pnr-MD237 E2fGD4448 visible, with Scer\GAL4ptc-559.1 E2fdsRNA.Scer\UAS.cJa E2fScer\UAS.cNa visible, with Scer\GAL4sca-T3 E2fScer\UAS.cNa visible, with Scer\GAL4wg.PM E2fScer\UAS.cNa visible | recessive E2fi1 E2fi2 wild-type E2fhs.PS Phenotype manifest in Allele 1st posterior cell, with Scer\GAL4ptc-559.1 E2fdsRNA.Scer\UAS.cJa abdomen, with Scer\GAL4ptc-559.1 E2fScer\UAS.cNa antenna, with Scer\GAL4[-]: E2fdsRNA.C.Scer\UAS antenna, with Scer\GAL4I-76-D E2fdsRNA.C.Scer\UAS anterior midgut primordium, with Scer\GAL4arm.PS E2fScer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP central nervous system E2frM729 chordotonal organ E2f91 chorion E2f91 E2fi1 E2fi2 dorsal appendage (with E2f07172) E2fi2 dorsal appendage (with E2fi2) E2f07172 egg E2fi1 egg (with E2f07172) E2fi2 egg (with E2fi2) E2f07172 embryonic/larval optic lobe E2f91 E2frM729 embryonic thorax & neuron E2f07172 epidermis E2f91 eye E2f07172 E2fi1 E2fi2 E2frM729 E2fsu89 eye, with Scer\GAL4[-]: E2fdsRNA.C.Scer\UAS eye, with E2f91 E2fhs.PD eye, with E2fdsRNA.Scer\UAS.cJa, Scer\GAL4GMR.PU E2fi1 eye, with E2fhs.PD E2f91 eye, with E2fi1, Scer\GAL4GMR.PU E2fdsRNA.Scer\UAS.cJa eye, with Scer\GAL4GMR.PF E2fScer\UAS.cNa eye, with Scer\GAL4GMR.PU E2fdsRNA.Scer\UAS.cJa eye, with Scer\GAL4I-76-D E2fdsRNA.C.Scer\UAS eye-antennal disc E2f07172 E2frM729 eye disc E2f91 E2frM729 eye disc & mitotic cell cycle E2f91 E2frM729 eye disc | somatic clone E2frM729 eye disc posterior to the morphogenetic furrow, with Scer\GAL4GMR.PU E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A-Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP eye photoreceptor cell E2f07172 follicle cell E2fi1 E2fi2 follicle stem cell | somatic clone E2fdsRNA.Scer\UAS.cDa heart primordium E2fdsRNA.cKa E2fdsRNA.si.cKa heterochromatin & nurse cell, with E2f91 E2fhs.PD heterochromatin & nurse cell, with E2fhs.PD E2f91 heterochromatin & nurse cell (with Df(3R)e-BS2) E2fi1 E2fi2 imaginal disc (with E2f91) E2frM729 imaginal disc (with E2frM729) E2f91 interommatidial bristle E2fhs.PA interommatidial bristle | ectopic E2fsu89 macrochaeta E2fBG01029 E2fBG01993 E2fBG02225 E2fBG02325 E2fi1 macrochaeta, with Scer\GAL4nos.PG E2fScer\UAS.cNa macrochaeta, with Scer\GAL4sca-T3 E2fScer\UAS.cNa mesothoracic tergum, with Scer\GAL4pnr-MD237 E2fGD4448 midgut primordium & S phase E2f164 mitotic cell cycle E2fdsRNA.cFa neuroblast E2frM729 neuroblast NB6-4 E2f07172 neuroblast NB6-4 of abdomen, with Scer\GAL4sca-537.4 E2fScer\UAS.cNa neuroblast NB6-4 of thorax | ectopic, with Scer\GAL4sca-537.4 E2fScer\UAS.cNa nuclear chromosome & nurse cell (with Df(3R)e-BS2) E2fi2 nuclear membrane & nurse cell (with Df(3R)e-BS2) E2fi1 E2fi2 nurse cell E2fi2 nurse cell (with Df(3R)e-BS2) E2fi1 E2fi2 nurse cell (with E2f07172) E2fi1 nurse cell (with E2fi1) E2f07172 ommatidium E2f07172 E2fhs.PA E2frM729 ommatidium, with Scer\GAL4GMR.PU E2fdsRNA.Scer\UAS.cJa oocyte (with E2f07172) E2fi2 oocyte (with E2fi2) E2f07172 ovary (with Df(3R)e-BS2) E2fi1 E2fi2 peripheral nervous system E2f91 photoreceptor cell E2frM729 polar body nucleus (with Df(2R)vg56) E2fi2 posterior midgut primordium, with Scer\GAL4arm.PS E2fScer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP rhabdomere E2f07172 E2frM729 trichogen cell, with Scer\GAL4pnr-MD237 E2fGD4448 wing, with Scer\GAL4Act88F.PD E2fScer\UAS.cNa wing, with Scer\GAL4en-e16E E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP wing blade, with Scer\GAL4Act88F.PD E2fScer\UAS.cNa wing blade, with Scer\GAL4wg.PM E2fScer\UAS.cNa wing disc | posterior | third instar larval stage, with Scer\GAL4en-e16E E2fScer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fDBD.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fRb.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fTrunc.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A-Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP E2fPIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP wing disc | posterior compartment, with Scer\GAL4en-e16E E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP wing disc | somatic clone E2frM729 wing disc | wandering third instar larval stage E2fi2 E2fsu89 wing pouch, with Scer\GAL4Act88F.PD E2fScer\UAS.cNa wing vein, with Scer\GAL4en-e16E E2fScer\UAS.cNa
Classical Alleles ( 52 )
For All Classical Alleles Show
Allele of E2f Class Mutagen Stocks Known lesion E2f07172 loss of function allele P-element activity 2 Yes E2fLA00365 P-element activity 2 -- E2frM729 loss of function allele P-element activity 2 Yes E2f5-HA-1661 1 -- E2fc00310 1 -- E2fc06065 piggyBac transposase 1 -- E2fCB-0495-3 1 -- E2fd01508 P-element activity 1 -- E2fd02846 P-element activity 1 -- E2fd03985 P-element activity 1 -- E2fEY05005 P-element activity 1 -- E2fEY14408 P-element activity 1 -- E2ff01550 piggyBac transposase 1 -- E2ff01988 piggyBac transposase 1 -- E2ff05222 piggyBac transposase 1 -- E2ff05965 piggyBac transposase 1 -- E2fGS3152 1 Yes E2fGS9647 1 Yes E2fi2 hypomorphic allele - genetic evidence ethyl methanesulfonate 1 Yes E2fMI00618 1 -- E2fMI03553 1 -- E2fMI07609 1 -- E2fNP3094 1 -- E2f91 amorphic allele - genetic evidence, loss of function allele ethyl methanesulfonate 0 Yes E2f155 ethyl methanesulfonate 0 -- E2f164 hypomorphic allele - genetic evidence P-element activity 0 -- E2f296 ethyl methanesulfonate 0 Yes E2f337 ethyl methanesulfonate 0 -- E2f5-SZ-3242 0 -- E2fBG01029 P-element activity 0 Yes E2fBG01993 P-element activity 0 Yes E2fBG02225 P-element activity 0 Yes E2fBG02325 P-element activity 0 Yes E2fc00336 0 -- E2fd02760 P-element activity 0 -- E2fd05852 P-element activity 0 -- E2fd06292 P-element activity 0 -- E2fDG02609 P-element activity 0 -- E2fE(Sev-CycE)3A ethyl methanesulfonate 0 -- E2fEP3029 P-element activity 0 -- E2fEY08459 P-element activity 0 -- E2fEY16535 P-element activity 0 -- E2ff06935 piggyBac transposase 0 -- E2fi1 ethyl methanesulfonate 0 Yes E2fj3B1 P-element activity 0 -- E2fj3C2 P-element activity 0 -- E2fKG03332 P-element activity 0 -- E2fmir-11-KO.w+ ends-out gene targeting FLPase SCEI endonuclease 0 Yes E2fmir-11-R.w- site specific recombinase 0 Yes E2fsu89 0 Yes E2funspecified 0 -- E2fΔ1 FLPase 0 Yes
Alleles Carried on Transgenic Constructs ( 41 )
For All Alleles Carried on Transgenic Constructs Show
Allele of E2f Class Mutagen Stocks Known lesion E2fScer\UAS.cNa in vitro construct - regulatory fusion 4 Yes E2fGD4448 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes E2fHMS01541 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes E2fJF01741 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes E2fJF02718 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes E2fPIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 1 Yes E2fScer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes E2f336-805.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes E2f336-805.Scer\UAS in vitro construct - regulatory fusion 0 Yes E2f560-805.Act5C.T:Scer\GAL4 in vitro construct - coding region fusion 0 Yes E2f769-805.Act5C.T:Scer\GAL4 in vitro construct - coding region fusion 0 Yes E2fAct5C.PD in vitro construct - regulatory fusion 0 Yes E2fAct5C.PO in vitro construct - regulatory fusion 0 Yes E2fcFa in vitro construct 0 Yes E2fDBD.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes E2fdl560.Act5C in vitro construct - deletion 0 Yes E2fdl769.Act5C in vitro construct - deletion 0 Yes E2fdsRNA.A.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes E2fdsRNA.B.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes E2fdsRNA.C.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes E2fdsRNA.cFa in vitro construct - RNAi 0 Yes E2fdsRNA.cKa in vitro construct - RNAi 0 Yes E2fdsRNA.cLa in vitro construct - RNAi 0 Yes E2fdsRNA.Scer\UAS.cDa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes E2fdsRNA.Scer\UAS.cJa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes E2fdsRNA.si.cKa in vitro construct - RNAi 0 Yes E2ffl.cCa in vitro construct 0 Yes E2fGMR.PD in vitro construct - regulatory fusion 0 Yes E2fhs.PA in vitro construct - regulatory fusion 0 Yes E2fhs.PD in vitro construct - regulatory fusion 0 Yes E2fhs.PS in vitro construct - regulatory fusion 0 Yes E2fie1.PF in vitro construct - regulatory fusion 0 Yes E2fKK100304 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes E2fMtnA.PH in vitro construct - regulatory fusion 0 Yes E2fPIP-3A-DBD.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes E2fPIP-3A-Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes E2fPIP-3A-Trunc.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes E2fPIP-7A.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes E2fPIP-7del.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes E2fRb.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes E2fTrunc.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)e-BS2 (Spradling et al., 1999, Royzman et al., 2002, BDGP Project Members, 1994-1999, Royzman et al., 1997) Df(3R)e-D7 (Duronio et al., 1995) Df(3R)ED6076 (Ryder and Roote, 2004.11.10) Df(3R)e-N19 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(3R)Exel6186 (Ryder, 2004.4.26)
Not disrupted in	Df(3R)e-F1 (BDGP Project Members, 1994-1999, Duronio et al., 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4448} NA P{KK100304} NA P{TRiP.HMS01541} NA P{TRiP.JF01741} NA P{TRiP.JF02718} NA P{UAS-E2f.336-805} NA P{UAS-E2f.A.RNAi} NA P{UAS-E2f.B.RNAi} NA P{UAS-E2f.C.RNAi} NA P{UAS-E2f.dsRNA.J} NA P{UAS-E2f.dsRNA} NA P{UAS-E2f.N} NA P{UAS-HA-E2f.336-805} NA P{UASp-GFP-E2f1.PIP-3A} NA P{UASp-GFP-E2f1.PIP-7A} NA P{UASp-GFP-E2f1.PIP-7del} NA P{UASp-GFP-E2f1.WT} NA P{UASp-GFP-E2f.DBD} NA P{UASp-GFP-E2f.PIP-3A-DBD} NA P{UASp-GFP-E2f.PIP-3A-Rb} NA P{UASp-GFP-E2f.PIP-3A-Trunc} NA P{UASp-GFP-E2f.Rb} NA P{UASp-GFP-E2f.Trunc} NA reporter construct P{E2f-lacZ.-2184+217} No P{E2f-lacZ.cadmut} No heat-shock construct P{hs-dE2F.D} NA P{hs-dE2F.S} NA P{hsp70-E2F} NA characterization construct P{GMRdE2F} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}E2fEP3029 NA P{EP}EP3707EP3707 NA P{EP}InREP3180 NA P{EPgy2}E2fEY05005 NA P{EPgy2}E2fEY08459 NA P{EPgy2}E2fEY14408 NA P{EPgy2}E2fEY16535 NA P{GSV1}C211C211 NA P{GSV1}E2fGS3152 NA P{GSV6}E2fGS9647 NA P{Mae-UAS.6.11}B0748B0748 NA P{Mae-UAS.6.11}E2fLA00365 NA P{XP}E2fd01508 NA P{XP}E2fd02760 NA P{XP}E2fd02846 NA P{XP}E2fd03985 NA P{XP}E2fd05852 NA P{XP}E2fd06292 NA miscellaneous insertions Mi{MIC}E2fMI00618 NA Mi{MIC}E2fMI03553 NA Mi{MIC}E2fMI07609 NA P{GMroX1}93E NA P{GT1}E2fBG01029 NA P{GT1}E2fBG01993 NA P{GT1}E2fBG02225 NA P{GT1}E2fBG02325 NA P{hsneo.ry+}E2f164 NA P{RS3}E2fCB-0495-3 NA P{RS5}E2f5-HA-1661 NA P{RS5}E2f5-SZ-3242 NA P{SUPor-P}E2fKG03332 NA P{wHy}E2fDG02609 NA PBac{PB}E2fc00310 NA PBac{PB}E2fc00336 NA PBac{PB}E2fc06065 NA PBac{WH}E2ff01550 NA PBac{WH}E2ff01988 NA PBac{WH}E2ff05222 NA PBac{WH}E2ff05965 NA PBac{WH}E2ff06935 NA insertion of enhancer trap binary system P{GawB}E2fNP3094 No insertion of enhancer trap P{lacW}E2fj3B1 No P{lacW}E2fj3C2 No P{PZ}E2f07172 No P{PZ}E2frM729 No
Gene Ontology: Function, Process & Cellular Component ( 30 unique terms )
Terms Based on Experimental Evidence ( 22 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from direct assay (Ohtani and Nevins, 1994) protein binding inferred from physical interaction with dom (Lu et al., 2007) inferred from physical interaction with l(3)mbt (Lu et al., 2007) inferred from physical interaction with Rbf (Du et al., 1996) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription inferred from direct assay (Stevaux et al., 2002) contributes_to sequence-specific DNA binding inferred from physical interaction with Dp (Sawado et al., 1998) contributes_to sequence-specific DNA binding RNA polymerase II transcription factor activity inferred from physical interaction with Dp (Dynlacht et al., 1994) sequence-specific DNA binding transcription factor activity inferred from direct assay (Ohtani and Nevins, 1994) transcription factor binding inferred from physical interaction with Dp (Dynlacht et al., 1994) inferred from physical interaction with Rbf (Stevaux et al., 2002)
Biological Process
CV term Evidence References
antimicrobial humoral response inferred from mutant phenotype (Kleino et al., 2005) cellular process inferred from mutant phenotype (Boutros et al., 2004) dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) DNA endoreduplication inferred from genetic interaction with CycE AND inferred from mutant phenotype (Weng et al., 2003) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Carreira et al., 2011) muscle tissue development inferred from mutant phenotype (Parrish et al., 2006) negative regulation of transcription from RNA polymerase II promoter inferred from direct assay (Sawado et al., 1998) neuron development inferred from mutant phenotype (Parrish et al., 2006) positive regulation of gene expression inferred from mutant phenotype (Lu et al., 2007) positive regulation of nurse cell apoptotic process inferred from mutant phenotype (Royzman et al., 2002) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Ohtani and Nevins, 1994, Sawado et al., 1998) regulation of cell cycle inferred from mutant phenotype (Royzman et al., 1997) regulation of hemocyte proliferation inferred from mutant phenotype (Sinenko et al., 2010)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Ohtani and Nevins, 1994) Rb-E2F complex inferred from direct assay (Du et al., 1996, Stevaux et al., 2002)
Terms Based on Predictions or Assertions ( 11 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding transcription factor activity inferred from sequence or structural similarity with human E2F1 (Ohtani and Nevins, 1994)
Biological Process
CV term Evidence References
eggshell chorion gene amplification traceable author statement (McNairn and Gilbert, 2003, Lee and Orr-Weaver, 2003) G1/S transition of mitotic cell cycle traceable author statement (Lee and Orr-Weaver, 2003) growth non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) imaginal disc development traceable author statement (Lee and Orr-Weaver, 2003) nurse cell apoptotic process traceable author statement (Lee and Orr-Weaver, 2003) positive regulation of cell proliferation non-traceable author statement (Potter and Xu, 2001) positive regulation of transcription, DNA-dependent traceable author statement (Lee and Orr-Weaver, 2003) regulation of cell cycle non-traceable author statement (Weinkove and Leevers, 2000) regulation of transcription involved in G1/S phase of mitotic cell cycle non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Dyson, 1994.6.6, Swiss-Prot Project Members, 1997.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
E2f - Dp anti bait coimmunoprecipitation, western blot (Du et al., 1996) E2f - Rbf anti bait coimmunoprecipitation, western blot, two hybrid (Du et al., 1996)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions E2f allele Gene References E2f91 CycD (Emmerich et al., 2004) CycE (Secombe et al., 1998, Royzman et al., 1999) Dp (Frolov et al., 2001) E2f2 (Frolov et al., 2001) Rbf (Du, 2000) scrib (Brumby and Richardson, 2003) E2f164 Rbf (Du, 2000) E2f07172 E2f2 (Frolov et al., 2001) E2fdsRNA.cFa dap (Frolov et al., 2003) E2fE(Sev-CycE)3A CycE (Lane et al., 2000) E2fGMR.PD brm (Staehling-Hampton et al., 1999) Dp (Du et al., 1996, Du and Dyson, 1999, Staehling-Hampton et al., 1999, Kuzin et al., 2000) E2f2 (Frolov et al., 2001) E(E2f)2B (Staehling-Hampton et al., 1999) ebi (Boulton et al., 2000) Egfr (Staehling-Hampton et al., 1999) ksh-15 (Staehling-Hampton et al., 1999) ksh-16 (Staehling-Hampton et al., 1999) ksh-17 (Staehling-Hampton et al., 1999) ksh-18 (Staehling-Hampton et al., 1999) ksh-19 (Staehling-Hampton et al., 1999) mor (Staehling-Hampton et al., 1999) osa (Staehling-Hampton et al., 1999) pjc-16 (Staehling-Hampton et al., 1999) pnt (Staehling-Hampton et al., 1999) Ras85D (Staehling-Hampton et al., 1999) rl (Staehling-Hampton et al., 1999) spen (Staehling-Hampton et al., 1999) E2fhs.PA CycA (Hayashi and Yamaguchi, 1999) E2fhs.PD Aats-asp (Galloni and Edgar, 1999) blw (Galloni and Edgar, 1999) bonsai (Galloni and Edgar, 1999) Dp (Duronio et al., 1996) eIF-4a (Galloni and Edgar, 1999) milou (Galloni and Edgar, 1999) RpL30 (Galloni and Edgar, 1999) trol (Caldwell and Datta, 1998) E2fi1 CycE (Royzman et al., 1999) E2fi2 Rbf (Du, 2000) E2frM729 CycE (Secombe et al., 1998) Dp (Frolov et al., 2001) E2f2 (Frolov et al., 2001) Rbf (Du, 2000) E2fScer\UAS.cNa Dp (Neufeld et al., 1998) E2fsu89 Rbf (Weng et al., 2003) E2funspecified CycE (Brumby et al., 2004) unspecified ban (Brennecke et al., 2003) CycE (Duronio et al., 1996, Royzman et al., 1997) Nsf2 (Laviolette et al., 2005) pbl (Gregory et al., 2007) Rbf (Kuzin et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67516 DroID - A comprehensive database of gene and protein interactions. • FBgn0011766 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42550
Orthologs
OrthoDB Orthologs (30) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6F7P51 Dipteran inclusive ortholog search EOG6Z36PC Insect inclusive ortholog search EOG6ZGN7X Arthropod inclusive ortholog search EOG6S4MXM Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6F7P51)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\E2f     Drosophila simulans   Dsim\GD19970 Y   Drosophila sechellia   Dsec\GM11000 Y   Drosophila erecta   Dere\GG14417 Y   Drosophila yakuba   Dyak\GE24969 Y   Drosophila ananassae   Dana\GF17810 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19549 Y   Drosophila persimilis   Dper\GL21507 Y   Drosophila willistoni   Dwil\GK12076 Y   Drosophila virilis   Dvir\GJ23948 Y   Drosophila mojavensis   Dmoj\GI23766 Y   Drosophila grimshawi   Dgri\GH19495 Y
Orthologs in non-Drosophila Dipterans (EOG6Z36PC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012817   Anopheles gambiae Malaria mosquito  Agam\AGAP001978   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006972
Orthologs in non-Dipteran Insects (EOG6ZGN7X)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB42706   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG006113   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17375   Atta cephalotes Leafcutter ant  Acep\ACEP26092   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15716   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20414   Linepithema humile Argentine ant  Lhum\LH16032   Pogonomyrmex barbatus Red harvester ant  Pbar\PB14445   Solenopsis invicta Red fire ant  Sinv\SINV25323   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004845   Bombyx mori Silkmoth  Bmor\BmE2F1   Pediculus humanus Human body louse  Phum\PHUM220810   Tribolium castaneum Red flour beetle  Tcas\TC005074
Orthologs in non-Insect Arthropods (EOG6S4MXM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\D18Ertd653e   Ixodes scapularis Deer tick  Isca\ISCW005631
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19970 Drosophila sechellia Dsec\GM11000 Drosophila erecta Dere\GG14417 Drosophila yakuba Dyak\GE24969 Drosophila ananassae Dana\GF17810 Drosophila pseudoobscura pseudoobscura Dpse\GA19549 Drosophila persimilis Dper\GL21507 Drosophila willistoni Dwil\GK12076 Drosophila virilis Dvir\GJ23948 Drosophila mojavensis Dmoj\GI23766 Drosophila grimshawi Dgri\GH19495
Stocks & Reagents
Stocks Listed in FlyBase ( 37 )
Bloomington	34058 w*; P{UASp-GFP-E2f1.WT}4
Harvard	- P{XP}E2fd01508 - P{XP}E2fd02846 - P{XP}E2fd03985 - PBac{PB}E2fc00310 - PBac{PB}E2fc06065 - PBac{WH}E2ff01550 - PBac{WH}E2ff01988 - PBac{WH}E2ff05222 - PBac{WH}E2ff05965
VDRC	v15886 w1118; P{GD4448}v15886 v15887 w1118; P{GD4448}v15887
	More stocks available...
Genomic Clones ( 2 )
	BACR16N15 BACR48I01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 118 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH16721 LD35741 LP10386
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04650 AT11090 GH19288 GH19910 GM02934 GM03777 HL02884 LD07206 LD19621 LD20604 LD21333 LD23115 LD23243 LD30729 LD33721 LD36810 LD39013 LD40411 LD40445 LD41939 LD45714 LD46208 LP04388 RE02470 RE19442 RE31094 RE35449 RE42547 SD13982 SD18176 SD28227
Other clones	1618 32726 33799 33965 34073 45708 51269 55776 57407 67569 76673 97736 100796 102693 102748 164503 176260 214319.3 282378 287997 294795 298326 CK01.17A.B8 CK01.64C.F6 CK01.84C.D1 CK01.86A.B12 CK01.99B.D10 CK01.108D.C2 CK01.109C.B12 CK01.115D.C5 CK01.170B.D3 CK00303 Dmel_dig_008010_0712_0421 Dmel_dig_014980_0714_2724 Dmel_dig_015563_0637_2302 Dmel_dig_016316_0634_1727 Dmel_dig_019363_0657_3238 EC053493 EC251876 EC255877 EC259802 EK030009 EK067010 EK067940 EK098141 EK099038 EK104414 EK142931 EK148436 EK148644 EK158660 EK162001 EK162210 EK173009 EK183925 EK184031 EK195755 EK196863 EK206622 EK206926 EK208524 EK217101 EK223111 EK226045 EK268914 EK276842 EK294205 EK307701 EK307745 EN02947 EN13318 EN15329 EP03943 EP04903 EP04931 EP11214 EP11505 EP13439 EP17362 FGM313C03 IP16825 LD24268 LD36172 UUGC1042
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011766 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42550
Antibody Information
	polyclonal (Korenjak et al., 2012)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	mir-998 is located within an intron of E2f. (Stark et al., 2007) mir-998 and mir-11 are both located in the same intron of E2f. (Stark et al., 2007)
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. RNAi of E2f results in increased arborization of ddaD and ddaE neurons. RNAi also causes defects in muscle, alterations in the number of MD neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development. (Parrish et al., 2006) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells. (Kleino et al., 2005) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) E2f as part of the E2f-Rb complexes has a role in the down regulation of CycE during the gap phase of endocycling cells in normal development. (Weng et al., 2003) E2f transcriptional activity in nurse cells is essential for thr robust synthesis of S phase transcripts that are deposited into the oocyte. E2f is needed to limit the replication of heterochromatin in nurse cells. E2f is required for nurse cell nuclear envelope breakdown and apoptosis. (Royzman et al., 2002) Identified with: LP10386 (BDGP-DGC) <up>FlyBase curator comment: EST subsequently found to be chimeric</up>. (BDGP Project Members, 2000-) Most, if not all, of the function of Rbf during development is mediated through E2f. E2f functions primarily as a transcription activator rather than a co-repressor of Rbf during development. (Du, 2000) Identification: One of a collection of genes identified with defective larval growth that extend larval life. (Galloni and Edgar, 1999) Both Dp and E2f are necessary for viability and mutations in the genes cause lethality at the larval\pupal stage. E2f is required for wild-type growth in mitotic and polytene tissues. Mutant phenotypes reveal that both genes promote progression of the cell cycle. (Royzman et al., 1997) In vitro binding studies of E2f and Dp to the DNApol-α180 promoter region reveals each of the E2f binding sites plays a distinct role as positive or negative element in the regulation of the DNApol-α180 promoter during development. (Yamaguchi et al., 1997) Endogenous E2f falls from high to very low levels as cells initiate DNA synthesis during a developmentally regulated G1-S transition in the eye disc. Ectopic E2f expression drives many otherwise quiescent cells to enter S phase, subsequently these cells express rpr and die. Ectopic E2f expression during the S phase in normally cycling cells blocks their re-entry into S phase in the following cell cycle. These results show that an elevation in the level of E2f is sufficient to induce imaginal disc cells to enter S phase. Also they suggest that the down regulation of E2f upon entry into S phase may be essential to prevent the induction of apoptosis. (Asano et al., 1996) E2f is required at multiple stages of development and may have an important function in post-mitotic cells in addition to its role during cell proliferation. (Brook et al., 1996) Ectopic expression of E2f together with Dp induces cell proliferation and cell death in the eye. (Du et al., 1996) E2f transcription factor-induced S phase requires CycE. (Duronio et al., 1996) Deduced protein sequence of transcripts from the E(var)93E locus identifies the E2f gene. (Seum et al., 1996) Mutations of E2f dominantly enhance position effect variegation, as tested with w and y variegating rearrangements. Overexpression suppresses position-effect variegation. (Seum et al., 1996) E2f is essential in most cells for a G1-S transcriptional program and for G1-S progression. (Duronio et al., 1995) During G1 of cycle 17 in the endocycling cells, E2f activation is independent of CycE, whereas CycE expression requires E2f. In the CNS CycE is expressed by a route independent of E2f, and the activation of E2f depends on CycE. The hierarchical relationship of CycE and E2f is reversed by tissue-specific distinctions in the mode of expression of CycE. (Duronio and O'Farrell, 1995) Analysis of expression patterns of E2f indicates in late stage embryos the protein is present in a restricted group of neural cells. In early embryos the protein is widely expressed in a segment restricted pattern. Distinct expression patterns of Dp and E2f suggest that the formation of Dp/E2f heterodimers is subject to complex regulatory cues. (Hao et al., 1995) A dominant modifier of position effect variegation. (Spierer et al., 1995) Dp and E2f gene products interact and cooperate to give sequence specific DNA binding and optimal trans-activation. This suggests that E2f may be composed of heterodimers and may be regulated by retinoblastoma proteins. (Dynlacht et al., 1994) The product of E2f binds to an E2F recognition site and can activate transcription of Ecol\CAT reporter plasmids in Drosophila cells. The transcriptional activation function can be found in C-terminal sequences. A potential gene target for E2f is DNA-polymerase α. (Ohtani and Nevins, 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079713 NM_169961 NM_169962 NM_001275880 RefSeq (Proteins) • NP_524437 NP_732646 NP_732647 NP_001262809 DDBJ / EMBL / GenBank • AA695506 AAA19003 AAF55904 AAL39294 AAN13878 AAN13879 AAV36981 AB011813 AGB96189 AI296470 AI386589 AW940634 AY069149 BAA32746 BT003553 BT016096 CAA55186 CL528112 CL610161 CL943631 CZ470466 CZ472277 CZ472982 CZ473028 CZ473672 CZ474760 CZ474761 CZ475036 CZ484015 CZ484485 CZ487849 CZ488593 CZ489675 DU708632 S82201 S82217 U10184 X78421 Entrez Gene - A searchable database of RefSeq genes. • 42550 UniProtKB/Swiss-Prot • Q27368 UniProtKB/TrEMBL • Q8T0P0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor E2F/dimerisation partner (TDP) (IPR003316) Winged helix-turn-helix DNA-binding domain (IPR011991) E2F Family (IPR015633) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH16721
Linkouts
	BioGRID - A database of protein and genetic interactions • 67516 DroID - A comprehensive database of gene and protein interactions. • FBgn0011766 DRSC - Results from RNAi screens. • FBgn0011766 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011766 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6376-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011766 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42550 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/e2f.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42550 modMine - Data generated by the modENCODE project. • FBgn0011766 REDfly - A database of transcriptional regulatory elements. • FBgn0011766
Synonyms & Secondary IDs ( 29 )
Reported As
Symbol Synonym	CG6376 (Kim et al., 2004, Norga et al., 2003, Laviolette et al., 2005, Quinones-Coello, 2007, Molnar et al., 2006, Grieder et al., 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Ni et al., 2011.7.19) dE2F (Berger et al., 2005, Chia and Prokopenko, 2005, Jorgensen and Tyers, 2004, Secombe et al., 2004, Hwang et al., 2002, Yoshida et al., 2001, Kwon et al., 2001, Cayirlioglu and Duronio, 2001, Coelho and Leevers, 2000, Whittaker et al., 2000, Kuzin et al., 2000, Myster and Duronio, 2000, Cayirlioglu et al., 2000, Weinkove and Leevers, 2000, Seum, 1997.2.11, Seum, 1997.2.11, Staehling-Hampton et al., 1999, Hirose et al., 1999, Royzman et al., 1999, Hayashi and Yamaguchi, 1999, Royzman and Orr-Weaver, 1998, Tao et al., 1999, Polymenis and Schmidt, 1999, Neufeld and Edgar, 1998, Duronio, 1999, Du and Dyson, 1999, Milan, 1998, Sawado et al., 1998, Sawado et al., 1998, Dyson, 1998, Skaer, 1998, Secombe et al., 1998, Neufeld et al., 1998, Johnston and Edgar, 1998, Britton and Edgar, 1998, Duronio et al., 1998, Secombe et al., 1998, Duronio et al., 1998, Neufeld et al., 1998, Helin, 1998, Duronio et al., 1998, Secombe et al., 1997, Duronio et al., 1996, De Rubertis et al., 1996, Du et al., 1996, Brook et al., 1996, Seum et al., 1996, Du et al., 1996, Du and Dyson, 1996, Yamaguchi et al., 1995, Duronio and O'Farrell, 1995, Duronio et al., 1995, Rowland and Bernards, 2006, Kohzaki and Murakami, 2005, Chia et al., 2008, Herranz et al., 2008, Herranz et al., 2010) dE2f (Sasaki et al., 1999, Secombe et al., 2007, Suzuki et al., 2009, Baonza and Freeman, 2005) dE2f1 (Korenjak and Brehm, 2005, Moon et al., 2005, Morris and Dyson, 2004, Ji et al., 2012, Morris et al., 2006, Li and Lu, 2009) dE2F1 (Coffman, 2004, Attwooll, 2004, Lipsick, 2004, Zraly et al., 2004, Reis and Edgar, 2003, Lee and Orr-Weaver, 2003, Legent et al., 2004, Johnston and Sanders, 2003, Thacker et al., 2003, Weng et al., 2003, Bosco et al., 2001, Royzman et al., 2002, Reis, 2003, Dimova et al., 2003, Du et al., 2003, Morris et al., 2002, Reis and Edgar, 2002, Lee et al., 2001, Park et al., 2001, Harbour and Dean, 2000, Lane et al., 2000, Du, 1999, Ji et al., 2006, Shi and Krek, 2007, Tountas and Fortini, 2007, Legent et al., 2006, Delanoue et al., 2004, Truscott et al., 2011, Hartl et al., 2007, Kugler and Nagel, 2007, Taylor-Harding et al., 2004, Morris et al., 2006, Milet et al., 2010, Ambrus and Frolov, 2010, Morris et al., 2008, Lu et al., 2007, Sun et al., 2008, Ahlander et al., 2008, Goulev et al., 2008, Rimkus et al., 2008, Escudero and Freeman, 2007, Steele et al., 2009, Baonza and Freeman, 2005, Jin et al., 2008, Lee et al., 2010, Moon et al., 2008, Hsieh et al., 2010, Legent et al., 2006, Herranz et al., 2010, Korenjak et al., 2012, Miles et al., 2012) de2f1 (Moon et al., 2004, Frolov et al., 2004, Dimova et al., 2003, Frolov et al., 2003, Frolov et al., 2003, Frolov et al., 2002, Frolov et al., 2001, Du, 2000, Frolov et al., 2007, Rowland and Bernards, 2006, Herr et al., 2007, Moon et al., 2005, Frolov et al., 2005, Rasheva et al., 2006, Moon et al., 2006, Ambrus et al., 2007, Moon et al., 2008, Nicolay and Frolov, 2008, Longworth et al., 2008, Steele et al., 2009, Ambrus et al., 2009, Nicholson et al., 2009, Li et al., 2010, Nicolay et al., 2010, Hsieh et al., 2010, Korenjak et al., 2012) def21 (Reis and Edgar, 2004) DmE2F-1 (Hao et al., 1995) Dp (Glavic et al., 2009) drosE2F1 (Nevins, 1994.5.31, Ohtani and Nevins, 1994, ) DRTF1/E2F (Hao et al., 1995) E2F (Kleino et al., 2005, Bandura, 2005, Kearney et al., 2004, Tower, 2004, Davidson, 2003, Ryoo and Steller, 2003, Moon et al., 2004, Kupsco et al., 2004, Bergmann and Lane, 2003, Kramer et al., 2003, Thacker et al., 2003, Potter and Xu, 2001, Frolov et al., 2001, Oldham et al., 2000, Datar et al., 2000, Manak et al., 2000, Li and Vaessin, 2000, Sasaki, 1998.3.3, Dyson, 1994.6.6, Enikolopov et al., 1999, Hinds, 1999, Asano and Wharton, 1999, Whittaker et al., 1999, Hayashi and Yamaguchi, 1999, Galloni and Edgar, 1999, Datar and Edgar, 1999, Caldwell and Datta, 1998, Su and O'Farrell, 1998, Britton and Edgar, 1998, Wallrath, 1998, Sass and Henikoff, 1998, Follette et al., 1998, Yamaguchi et al., 1997, Royzman et al., 1997, Lin et al., 1996, Royzman et al., 1997, Asano et al., 1996, Dynlacht et al., 1994, Frolov et al., 2007, Umetsu et al., 2006, Thomas, 2005, Kohzaki and Murakami, 2005, Mukherjee et al., 2006, Volkova et al., 2004, Sandmann et al., 2007, Buttitta and Edgar, 2006, Alvarez-Garcia and Miska, 2005, Sundaram, 2005, Parrish et al., 2006, Oktaba et al., 2008, Folberg-Blum et al., 2002, Hirose et al., 2001, Turner et al., 2011, Slattery et al., 2011, Chen et al., 2011, Lyulcheva et al., 2008, Mitchell et al., 2008, Glavic et al., 2009, Heriche et al., 2003, Buttitta et al., 2010, Lee et al., 2010, Herranz et al., 2010, Korenjak et al., 2012, Ge et al., 2012, Wang et al., 2012, Valadez-Graham et al., 2012) e2f (Zhou and Steller, 2003, Fung et al., 2003, Ge et al., 2012, Sinenko et al., 2010) E2f (Boulton et al., 2000, Laviolette et al., 2005, Gregory et al., 2007, Badal et al., 2006, Richter et al., 2011, Zhai et al., 2012, Quinones-Coello, 2007, Arancio et al., 2010, Stark et al., 2007, Molnar et al., 2006, Grieder et al., 2007, Isogai et al., 2007, Baxley et al., 2011, Sinenko et al., 2010, Southall and Brand, 2009, Choksi et al., 2006, Protzer et al., 2008, Sala et al., 2011, Morris et al., 2006, Liebl and Featherstone, 2005, Stark et al., 2007, Abruzzi et al., 2011, Carreira et al., 2011, Kozhevnikova et al., 2012) E2f1 (Muzzopappa and Wappner, 2005, Tower, 2004, Key et al., 2003, Shibutani et al., 2007, Kohzaki and Murakami, 2005, Cayirlioglu et al., 2003, Shibutani et al., 2007, Davidson et al., 2008, Geng and MacDonald, 2007, Morris et al., 2006, Shibutani et al., 2008, Khurana et al., 2006, Reddy et al., 2010, Acharya et al., 2010, Davidson and Duronio, 2012) E2F1 (Claycomb and Orr-Weaver, 2005, Emmerich et al., 2004, Brumby et al., 2004, Lewis et al., 2004, Bandura et al., 2005, Bandura et al., 2004, Xin et al., 2002, Cha et al., 2001, Edgar and Orr-Weaver, 2001, Sun and Deng, 2006, Morris and Dyson, 2007, Ji et al., 2007, Garcia et al., 2007, Sun et al., 2008, Zielke et al., 2008, Hyun et al., 2005, Maqbool et al., 2010, Herranz et al., 2008, Bowman et al., 2008, Zielke et al., 2008, Rogers et al., 2009, Mitchell et al., 2008, Tanaka-Matakatsu et al., 2009, Wang and Kalderon, 2009, Baonza and Freeman, 2005, Parakati and Dimario, 2005, Herranz et al., 2010) e2f1 (Brumby and Richardson, 2003, Buttitta et al., 2007, Buttitta et al., 2010, Gehrke et al., 2010, Miles et al., 2012) E2F-1 (McNairn and Gilbert, 2003, Taniue et al., 2010) E(Sev-CycE)3A (Lane et al., 2000) E(var)3-93E (Sun et al., 2000, Seum et al., 1996, Spierer et al., 1995) E(var)3-95E (Wallrath, 1998) E(var)93E   Evar(3)164 (Seum et al., 1995) l(3)07172   l(3)j3B1   l(3)j3C2   l(3)rM729
Name Synonym	E2F transcription factor   E2-promoter binding factor (Morris et al., 2006)
Secondary FlyBase IDs
	FBgn0010918 FBgn0011343 FBgn0011351 FBgn0011520 FBgn0011767 FBgn0013944 FBgn0016652
References ( 361 )
Generate a list of	All references relating to this gene ( 361 )
List References by type	Research paper  ( 203 ) Supplementary material  ( 9 ) Review  ( 50 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 20 ) Abstract  ( 60 ) FlyBase analysis  ( 3 ) Conference report  ( 6 ) DNA/RNA sequence record  ( 5 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 27 )
	Pitsouli and Perrimon, 2013, Sci. Signal. 6(263): ra12 The homeobox transcription factor cut coordinates patterning and growth during Drosophila airway remodeling. [FBrf0220848] Adamson and Lajeunesse, 2012, ScientificWorldJournal 2012: 347597 A Study of Epstein-Barr Virus BRLF1 Activity in a Drosophila Model System. [FBrf0218446] Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Bayarmagnai et al., 2012, Cell Cycle 11(22): 4191--4202 Drosophila GAGA factor is required for full activation of the dE2f1-Yki/Sd transcriptional program. [FBrf0220001] Davidson and Duronio, 2012, PLoS Genet. 8(8): e1002831 S phase-coupled e2f1 destruction ensures homeostasis in proliferating tissues. [FBrf0219273] Fisher et al., 2012, BMC Genomics 13: 506 Advances in genome-wide RNAi cellular screens: a case study using the Drosophila JAK/STAT pathway. [FBrf0220361] Ge et al., 2012, Cell Death Differ. 19(5): 839--846 Overlapping functions of microRNAs in control of apoptosis during Drosophila embryogenesis. [FBrf0217965] Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660 In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387 RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Kozhevnikova et al., 2012, Mol. Cell 47(1): 133--139 Metabolic Enzyme IMPDH Is Also a Transcription Factor Regulated by Cellular State. [FBrf0218906] Lee et al., 2012, Mol. Cell. Biol. 32(11): 2110--2120 A Dual Role for the dREAM/MMB Complex in the Regulation of Differentiation-Specific E2F/RB Target Genes. [FBrf0218296] Miles et al., 2012, Genes Dev. 26(4): 356--368 Pumilio facilitates miRNA regulation of the E2F3 oncogene. [FBrf0217481] Raj et al., 2012, Cell Cycle 11(20): 3731--3738 Rbf1 degron dysfunction enhances cellular DNA replication. [FBrf0219758] Valadez-Graham et al., 2012, Nucleic Acids Res. 40(4): 1460--1474 XNP/dATRX interacts with DREF in the chromatin to regulate gene expression. [FBrf0217551] Wang et al., 2012, Nat. Commun. 3: 769 Drosophila follicle stem cells are regulated by proliferation and niche adhesion as well as mitochondria and ROS. [FBrf0217898] Zhai et al., 2012, PLoS Genet. 8(3): e1002582 Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386 Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798] Baxley et al., 2011, Dev. Biol. 356(2): 398--410 The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis. [FBrf0214454] Carreira et al., 2011, BMC Dev. Biol. 11: 32 Genetic basis of wing morphogenesis in Drosophila: sexual dimorphism and non-allometric effects of shape variation. [FBrf0214273] Chen et al., 2011, PLoS ONE 6(4): e18853 Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster. [FBrf0213606] Nicolay et al., 2011, Genes Dev. 25(4): 323--335 Cooperation between dE2F1 and Yki/Sd defines a distinct transcriptional program necessary to bypass cell cycle exit. [FBrf0214348] Richter et al., 2011, Nat. Cell Biol. 13(9): 1029--1039 The tumour suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements. [FBrf0215050] Sala et al., 2011, EMBO J. 30(9): 1766--1777 Genome-wide characterization of chromatin binding and nucleosome spacing activity of the nucleosome remodelling ATPase ISWI. [FBrf0213629] Slattery et al., 2011, PLoS ONE 6(4): e14686 Genome-wide tissue-specific occupancy of the hox protein ultrabithorax and hox cofactor homothorax in Drosophila. [FBrf0213426] Truscott et al., 2011, Genes Dev. 25(17): 1820--1834 mir-11 limits the proapoptotic function of its host gene, dE2f1. [FBrf0215211] Turner et al., 2011, PLoS Genet. 7(3): e1001336 Population-Based Resequencing of Experimentally Evolved Populations Reveals the Genetic Basis of Body Size Variation in Drosophila melanogaster. [FBrf0213327] Zielke et al., 2011, Nature 480(7375): 123--127 Control of Drosophila endocycles by E2F and CRL4CDT2. [FBrf0216829] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...