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Gene Dmel\Hem

General Information
Symbol	Dmel\Hem	Species	D. melanogaster
Name	HEM-protein	Annotation symbol	CG5837
Feature type	protein_coding_gene	FlyBase ID	FBgn0011771
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	kette, kte, Nap1
Genomic Location
Chromosome (arm)	3L	Recombination map	3-47 +/- 1
Cytogenetic map	79D4-79D4	Sequence location	3L:22,277,454..22,281,299 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene HEM-protein is referred to in FlyBase by the symbol Dmel\Hem (CG5837, FBgn0011771). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence for 12 unique biological process terms, many of which group under: cellular component organization or biogenesis; anatomical structure development; cellular component organization or biogenesis at cellular level; organelle organization; actin filament-based process; cell projection organization; muscle cell differentiation; biological regulation; localization; regulation of developmental process; sensory organ development; central nervous system development. 22 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; nervous system; multi-cell-component structure; presumptive embryonic/larval nervous system; peripheral nervous system; sensillum; commissure; organelle; organ system; sense organ; non-connected developing system. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1630271_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Hem. Gene sequence location is 3L:22277454..22281299.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References Gautier et al., 2011, J. Cell Sci. 124(20): 3414--3427 D'Ambrosio and Vale, 2010, J. Cell Biol. 191(3): 471--478 Sequence features BKN20432 HFA11865 Controlled Vocabulary Terms cell morphogenesis
FB2011_10	References Jin et al., 2011, Dev. Cell 20(5): 623--638
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 79D4-79D4   Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
Experimentally Determined Cytological Location
Cytogenetic map Notes References 79E1-79E2     (Baumgartner, 1994.7.1) 79E1-79E2   (determined by in situ hybridisation)   (Spradling et al., 1999) 79E1-79E2   (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	3-47 +/- 1 (Hummel et al., 1999)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hem for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hem-RA FBtr0078510  NM_079490   3781   1126
Additional Transcript Data & Comments
Reported size (kB)	3.8 (northern blot) (Baumgartner et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hem-PA   FBpp0078162   129.4   1126   6.80     NP_524214   AAF51820
Additional Polypeptide Data & Comments
Reported size (kDa)	1126 (aa); 129 (kD predicted) (Baumgartner et al., 1995)
Comments	Antibody was raised against the peptide RHNDNPPLLKNKGC (Schenck et al., 2004)
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF51820   AI511911   AI511922   AI531827   AI534006   AQ026185   AW943661   AY118579 AAM49948   BI231683   BI235112   BI579834   BI633584   BI633616   BI635749   CA806992   CO177715   CO187078   CO263418   CO272866   CO279765   CO296258   CO302880   CO323379   EC060787   EC069617   EC079734   EC087290   EC196865   EC208240   EC209652   EC211844   EC240865   EC246548   EV588208   GH750917   GH753201   GH780159   GH782228   GH783051   GH784804   GH784875   GH792479   GH797669   GH802329   GH811052   GH811402   GH815347   GH817185   GH819593   GH820891   GH826172   GH829593   GH829640   GH842475   GH843660   GH859261   GH860036   GH861443   GH864560   GH866252   GH878717   GH889933   GH891694   GH894351   GH898372   GH901087   GH902372   GH902748   GH902953   GH905951   GH912372   GH919257   GH920097   GH926641   GH928166   GH928321   GH935179   GH936774   X80028 CAA56332
UniProtKB/Swiss-Prot	P55162
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval central nervous system   (Schenck et al., 2004) embryonic stage 1 -- 12   organism | ubiquitous   (Hummel et al., 2000) embryonic stage 13 -- 17   embryonic/larval central nervous system   (Hummel et al., 2000) VUM neuron   (Hummel et al., 2000) embryonic stage 14   supraesophageal ganglion   (Baumgartner et al., 1995) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Baumgartner et al., 1995) embryonic stage 1 -- 6   organism | ubiquitous   (Baumgartner et al., 1995) embryonic stage 7 -- 10   procephalic neuroblast   (Baumgartner et al., 1995) embryonic stage 13   organism | ubiquitous   (Baumgartner et al., 1995) embryonic stage 14   embryonic/larval pharynx   (Baumgartner et al., 1995) embryonic stage 14 -- 17   ventral nerve cord   (Baumgartner et al., 1995) embryonic stage 17   embryonic brain   (Baumgartner et al., 1995) adult stage & oogenesis stage S10   oocyte   (Baumgartner et al., 1995) follicle cell   (Baumgartner et al., 1995)
Additional Descriptive Data
	In Northern blots, Hem transcript is detected throughout development, with higher levels in the early embryo, in early first larval instar, and in adults. The expression detected in adults is likely due to ovarian accumulation. Hem transcript is observed in oogenesis and embryogenesis using in situ hybridization. In stage 10 oocytes, the follicle cell and the oocyte express Hem. Hem is ubiquitous in the early embryo. At embryonic stages 7-10, the presumptive procephalic neuroblasts express Hem. Expression is once again ubiquitous in stage 13 embryos. At stage 14, expression is strongest in the pharynx, supraoesophageal ganglion, and the ventral nerve cord. In stage 17 embryos, transcript is detected only in the brain and ventral cord. This pattern persists in the early larval stages. (Baumgartner et al., 1995)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 14 -- 16   commissure   (Schenck et al., 2004) embryonic stage 14 -- 17   longitudinal connective   (Schenck et al., 2004)   S2 cell-line   • actin cytoskeleton (Bogdan and Klambt, 2003) Comment:expression assayed in cultured S2 cells S2 cell-line   • cytoplasm (Bogdan and Klambt, 2003) Comment:expression assayed in cultured S2 cells S2 cell-line   • plasma membrane (Bogdan and Klambt, 2003) Comment:expression assayed in cultured S2 cells muscle cell   • actin cytoskeleton (Bogdan and Klambt, 2003) Comment:expression assayed in cultured muscle cells neuron   • axon & dendrite (Bogdan and Klambt, 2003) Comment:expression assayed in cultured neurons mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   • cytoplasm (Bogdan and Klambt, 2003) organism   • plasma membrane (Bogdan and Klambt, 2003) embryonic stage 12 -- 17     (Schenck et al., 2004)   cell   (Schenck et al., 2004) Comment:expression assayed in cultured S2 cells
Additional Descriptive Data
	Hem protein is present in longitudinal connectives and commissures during the establishment of the axonal network. (Schenck et al., 2004)
Marker for
	longitudinal connective
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0011771    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0011771 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0011771 expression level Developmental Stage   Expression Level embryo 00-02hr    1785 embryo 02-04hr    1555 embryo 04-06hr    3494 embryo 06-08hr    3265 embryo 08-10hr    3153 embryo 10-12hr    2674 embryo 12-14hr    2904 embryo 14-16hr    1626 embryo 16-18hr    1412 embryo 18-20hr    1070 embryo 20-22hr    775 embryo 22-24hr    1038 larva L1    1006 larva L2    932 larva L3 12hr old    723 larva L3 puffstage 1-2    962 larva L3 puffstage 3-6    1334 larva L3 puffstage 7-9    1767 white prepupae new    2037 white prepupae 12hr    2055 white prepupae 24hr    2246 pupae 2d postWPP    1562 pupae 3d postWPP    917 pupae 4d postWPP    470 adult male 01day    596 adult male 05day    588 adult male 30day    583 adult female 01day    881 adult female 05day    1675 adult female 30day    1570 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1785) embryo 02-04hr  (1555) embryo 04-06hr  (3494) embryo 06-08hr  (3265) embryo 08-10hr  (3153) embryo 10-12hr  (2674) embryo 12-14hr  (2904) embryo 14-16hr  (1626) embryo 16-18hr  (1412) embryo 18-20hr  (1070) embryo 20-22hr  (775) embryo 22-24hr  (1038) larva L1  (1006) larva L2  (932) larva L3 12hr old  (723) larva L3 puffstage 1-2  (962) larva L3 puffstage 3-6  (1334) larva L3 puffstage 7-9  (1767) white prepupae new  (2037) white prepupae 12hr  (2055) white prepupae 24hr  (2246) pupae 2d postWPP  (1562) pupae 3d postWPP  (917) pupae 4d postWPP  (470) adult male 01day  (596) adult male 05day  (588) adult male 30day  (583) adult female 01day  (881) adult female 05day  (1675) adult female 30day  (1570) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (1785) embryo 02-04hr  (1555) embryo 04-06hr  (3494) embryo 06-08hr  (3265) embryo 08-10hr  (3153) embryo 10-12hr  (2674) embryo 12-14hr  (2904) embryo 14-16hr  (1626) embryo 16-18hr  1412 embryo 18-20hr    1070 embryo 20-22hr    775 embryo 22-24hr    1038 larva L1    1006 larva L2    932 larva L3 12hr old    723 larva L3 puffstage 1-2    962 larva L3 puffstage 3-6    1334 larva L3 puffstage 7-9  (1767) white prepupae new  (2037) white prepupae 12hr  (2055) white prepupae 24hr  (2246) pupae 2d postWPP  (1562) pupae 3d postWPP    917 pupae 4d postWPP    470 adult male 01day    596 adult male 05day    588 adult male 30day    583 adult female 01day    881 adult female 05day  (1675) adult female 30day  (1570) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1785 embryo 02-04hr    1555 embryo 04-06hr    3494 embryo 06-08hr    3265 embryo 08-10hr    3153 embryo 10-12hr    2674 embryo 12-14hr    2904 embryo 14-16hr    1626 embryo 16-18hr    1412 embryo 18-20hr    1070 embryo 20-22hr    775 embryo 22-24hr    1038 larva L1    1006 larva L2    932 larva L3 12hr old    723 larva L3 puffstage 1-2    962 larva L3 puffstage 3-6    1334 larva L3 puffstage 7-9    1767 white prepupae new    2037 white prepupae 12hr    2055 white prepupae 24hr    2246 pupae 2d postWPP    1562 pupae 3d postWPP    917 pupae 4d postWPP    470 adult male 01day    596 adult male 05day    588 adult male 30day    583 adult female 01day    881 adult female 05day    1675 adult female 30day    1570 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1785 embryo 02-04hr    1555 embryo 04-06hr    3494 embryo 06-08hr    3265 embryo 08-10hr    3153 embryo 10-12hr    2674 embryo 12-14hr    2904 embryo 14-16hr    1626 embryo 16-18hr    1412 embryo 18-20hr    1070 embryo 20-22hr    775 embryo 22-24hr    1038 larva L1    1006 larva L2    932 larva L3 12hr old    723 larva L3 puffstage 1-2    962 larva L3 puffstage 3-6    1334 larva L3 puffstage 7-9    1767 white prepupae new    2037 white prepupae 12hr    2055 white prepupae 24hr    2246 pupae 2d postWPP    1562 pupae 3d postWPP    917 pupae 4d postWPP    470 adult male 01day    596 adult male 05day    588 adult male 30day    583 adult female 01day    881 adult female 05day    1675 adult female 30day    1570 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0011771 expression level Developmental Stage   Expression Level embryo 00-02hr    1785 embryo 02-04hr    1555 embryo 04-06hr    3494 embryo 06-08hr    3265 embryo 08-10hr    3153 embryo 10-12hr    2674 embryo 12-14hr    2904 embryo 14-16hr    1626 embryo 16-18hr    1412 embryo 18-20hr    1070 embryo 20-22hr    775 embryo 22-24hr    1038 larva L1    1006 larva L2    932 larva L3 12hr old    723 larva L3 puffstage 1-2    962 larva L3 puffstage 3-6    1334 larva L3 puffstage 7-9    1767 white prepupae new    2037 white prepupae 12hr    2055 white prepupae 24hr    2246 pupae 2d postWPP    1562 pupae 3d postWPP    917 pupae 4d postWPP    470 adult male 01day    596 adult male 05day    588 adult male 30day    583 adult female 01day    881 adult female 05day    1675 adult female 30day    1570 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1785) embryo 02-04hr  (1555) embryo 04-06hr  (3494) embryo 06-08hr  (3265) embryo 08-10hr  (3153) embryo 10-12hr  (2674) embryo 12-14hr  (2904) embryo 14-16hr  (1626) embryo 16-18hr  (1412) embryo 18-20hr  (1070) embryo 20-22hr  (775) embryo 22-24hr  (1038) larva L1  (1006) larva L2  (932) larva L3 12hr old  (723) larva L3 puffstage 1-2  (962) larva L3 puffstage 3-6  (1334) larva L3 puffstage 7-9  (1767) white prepupae new  (2037) white prepupae 12hr  (2055) white prepupae 24hr  (2246) pupae 2d postWPP  (1562) pupae 3d postWPP  (917) pupae 4d postWPP  (470) adult male 01day  (596) adult male 05day  (588) adult male 30day  (583) adult female 01day  (881) adult female 05day  (1675) adult female 30day  (1570) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  1785 embryo 02-04hr  1555 embryo 04-06hr  (3494) embryo 06-08hr  (3265) embryo 08-10hr  (3153) embryo 10-12hr  (2674) embryo 12-14hr  (2904) embryo 14-16hr  1626 embryo 16-18hr  1412 embryo 18-20hr    1070 embryo 20-22hr    775 embryo 22-24hr    1038 larva L1    1006 larva L2    932 larva L3 12hr old    723 larva L3 puffstage 1-2    962 larva L3 puffstage 3-6    1334 larva L3 puffstage 7-9  1767 white prepupae new  2037 white prepupae 12hr  2055 white prepupae 24hr  2246 pupae 2d postWPP  1562 pupae 3d postWPP    917 pupae 4d postWPP    470 adult male 01day    596 adult male 05day    588 adult male 30day    583 adult female 01day    881 adult female 05day  1675 adult female 30day  1570 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1785 embryo 02-04hr    1555 embryo 04-06hr    3494 embryo 06-08hr    3265 embryo 08-10hr    3153 embryo 10-12hr    2674 embryo 12-14hr    2904 embryo 14-16hr    1626 embryo 16-18hr    1412 embryo 18-20hr    1070 embryo 20-22hr    775 embryo 22-24hr    1038 larva L1    1006 larva L2    932 larva L3 12hr old    723 larva L3 puffstage 1-2    962 larva L3 puffstage 3-6    1334 larva L3 puffstage 7-9    1767 white prepupae new    2037 white prepupae 12hr    2055 white prepupae 24hr    2246 pupae 2d postWPP    1562 pupae 3d postWPP    917 pupae 4d postWPP    470 adult male 01day    596 adult male 05day    588 adult male 30day    583 adult female 01day    881 adult female 05day    1675 adult female 30day    1570 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1785 embryo 02-04hr    1555 embryo 04-06hr    3494 embryo 06-08hr    3265 embryo 08-10hr    3153 embryo 10-12hr    2674 embryo 12-14hr    2904 embryo 14-16hr    1626 embryo 16-18hr    1412 embryo 18-20hr    1070 embryo 20-22hr    775 embryo 22-24hr    1038 larva L1    1006 larva L2    932 larva L3 12hr old    723 larva L3 puffstage 1-2    962 larva L3 puffstage 3-6    1334 larva L3 puffstage 7-9    1767 white prepupae new    2037 white prepupae 12hr    2055 white prepupae 24hr    2246 pupae 2d postWPP    1562 pupae 3d postWPP    917 pupae 4d postWPP    470 adult male 01day    596 adult male 05day    588 adult male 30day    583 adult female 01day    881 adult female 05day    1675 adult female 30day    1570 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0011771    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0011771 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0011771 expression level Tissue   Expression Level Larval Central Nervous System    374.475 Larval Midgut    208.5 Larval Hindgut    234.3 Larval Malpighian Tubules    222.5 Larval Fat Body    169.9 Larval Salivary Gland    311.9 Larval Trachea    220.025 Larval Carcass    159.675 Adult Head    122.1 Adult Eye    116.3 Adult Brain    187.1 Adult Thoracic-Abdominal Ganglion    182.3 Adult Crop    138.5 Adult Midgut    163.7 Adult Hindgut    131.2 Adult Malpighian Tubules    109.1 Adult Fat Body    73.3 Adult Salivary Gland    177 Adult Heart    135.35 Adult VirginFemale Spermatheca    135.3 Adult InseminatedFemale Spermatheca    161.7 Adult Ovary    238.1 Adult Testis    49.5 Adult Male Accessory Gland    219 Adult Carcass    73.2 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    374.475 Larval Midgut    208.5 Larval Hindgut    234.3 Larval Malpighian Tubules    222.5 Larval Fat Body    169.9 Larval Salivary Gland    311.9 Larval Trachea    220.025 Larval Carcass    159.675 Adult Head    122.1 Adult Eye    116.3 Adult Brain    187.1 Adult Thoracic-Abdominal Ganglion    182.3 Adult Crop    138.5 Adult Midgut    163.7 Adult Hindgut    131.2 Adult Malpighian Tubules    109.1 Adult Fat Body    73.3 Adult Salivary Gland    177 Adult Heart    135.35 Adult VirginFemale Spermatheca    135.3 Adult InseminatedFemale Spermatheca    161.7 Adult Ovary    238.1 Adult Testis    49.5 Adult Male Accessory Gland    219 Adult Carcass    73.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    374.475 Larval Midgut    208.5 Larval Hindgut    234.3 Larval Malpighian Tubules    222.5 Larval Fat Body    169.9 Larval Salivary Gland    311.9 Larval Trachea    220.025 Larval Carcass    159.675 Adult Head    122.1 Adult Eye    116.3 Adult Brain    187.1 Adult Thoracic-Abdominal Ganglion    182.3 Adult Crop    138.5 Adult Midgut    163.7 Adult Hindgut    131.2 Adult Malpighian Tubules    109.1 Adult Fat Body    73.3 Adult Salivary Gland    177 Adult Heart    135.35 Adult VirginFemale Spermatheca    135.3 Adult InseminatedFemale Spermatheca    161.7 Adult Ovary    238.1 Adult Testis    49.5 Adult Male Accessory Gland    219 Adult Carcass    73.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    374.475 Larval Midgut    208.5 Larval Hindgut    234.3 Larval Malpighian Tubules    222.5 Larval Fat Body    169.9 Larval Salivary Gland    311.9 Larval Trachea    220.025 Larval Carcass    159.675 Adult Head    122.1 Adult Eye    116.3 Adult Brain    187.1 Adult Thoracic-Abdominal Ganglion    182.3 Adult Crop    138.5 Adult Midgut    163.7 Adult Hindgut    131.2 Adult Malpighian Tubules    109.1 Adult Fat Body    73.3 Adult Salivary Gland    177 Adult Heart    135.35 Adult VirginFemale Spermatheca    135.3 Adult InseminatedFemale Spermatheca    161.7 Adult Ovary    238.1 Adult Testis    49.5 Adult Male Accessory Gland    219 Adult Carcass    73.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0011771 expression level Tissue   Expression Level Larval Central Nervous System    374.475 Larval Midgut    208.5 Larval Hindgut    234.3 Larval Malpighian Tubules    222.5 Larval Fat Body    169.9 Larval Salivary Gland    311.9 Larval Trachea    220.025 Larval Carcass    159.675 Adult Head    122.1 Adult Eye    116.3 Adult Brain    187.1 Adult Thoracic-Abdominal Ganglion    182.3 Adult Crop    138.5 Adult Midgut    163.7 Adult Hindgut    131.2 Adult Malpighian Tubules    109.1 Adult Fat Body    73.3 Adult Salivary Gland    177 Adult Heart    135.35 Adult VirginFemale Spermatheca    135.3 Adult InseminatedFemale Spermatheca    161.7 Adult Ovary    238.1 Adult Testis    49.5 Adult Male Accessory Gland    219 Adult Carcass    73.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    374.475 Larval Midgut    208.5 Larval Hindgut    234.3 Larval Malpighian Tubules    222.5 Larval Fat Body    169.9 Larval Salivary Gland    311.9 Larval Trachea    220.025 Larval Carcass    159.675 Adult Head    122.1 Adult Eye    116.3 Adult Brain    187.1 Adult Thoracic-Abdominal Ganglion    182.3 Adult Crop    138.5 Adult Midgut    163.7 Adult Hindgut    131.2 Adult Malpighian Tubules    109.1 Adult Fat Body    73.3 Adult Salivary Gland    177 Adult Heart    135.35 Adult VirginFemale Spermatheca    135.3 Adult InseminatedFemale Spermatheca    161.7 Adult Ovary    238.1 Adult Testis    49.5 Adult Male Accessory Gland    219 Adult Carcass    73.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    374.475 Larval Midgut    208.5 Larval Hindgut    234.3 Larval Malpighian Tubules    222.5 Larval Fat Body    169.9 Larval Salivary Gland    311.9 Larval Trachea    220.025 Larval Carcass    159.675 Adult Head    122.1 Adult Eye    116.3 Adult Brain    187.1 Adult Thoracic-Abdominal Ganglion    182.3 Adult Crop    138.5 Adult Midgut    163.7 Adult Hindgut    131.2 Adult Malpighian Tubules    109.1 Adult Fat Body    73.3 Adult Salivary Gland    177 Adult Heart    135.35 Adult VirginFemale Spermatheca    135.3 Adult InseminatedFemale Spermatheca    161.7 Adult Ovary    238.1 Adult Testis    49.5 Adult Male Accessory Gland    219 Adult Carcass    73.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    374.475 Larval Midgut    208.5 Larval Hindgut    234.3 Larval Malpighian Tubules    222.5 Larval Fat Body    169.9 Larval Salivary Gland    311.9 Larval Trachea    220.025 Larval Carcass    159.675 Adult Head    122.1 Adult Eye    116.3 Adult Brain    187.1 Adult Thoracic-Abdominal Ganglion    182.3 Adult Crop    138.5 Adult Midgut    163.7 Adult Hindgut    131.2 Adult Malpighian Tubules    109.1 Adult Fat Body    73.3 Adult Salivary Gland    177 Adult Heart    135.35 Adult VirginFemale Spermatheca    135.3 Adult InseminatedFemale Spermatheca    161.7 Adult Ovary    238.1 Adult Testis    49.5 Adult Male Accessory Gland    219 Adult Carcass    73.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    122.1   NA  Eye    116.3   NA  Brain    187.1   374.475  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    182.3   NA  Crop    138.5   208.5  Midgut    163.7   234.3  Hindgut    131.2   222.5  Malpighian Tubules    109.1   169.9  Fat Body    73.3   311.9  Salivary Gland    177   NA  Heart    135.35   220.025  Trachea    NA   NA  VirginFemale Spermatheca    135.3   NA  InseminatedFemale Spermatheca    161.7   NA  Ovary    238.1   NA  Testis    49.5   NA  Male Accessory Gland    219   159.675  Carcass    73.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011771 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5837-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive Hem03335 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 HemGD11783 viable, with Scer\GAL4elav-C155 HemScer\UAS.cHa viable | poor HemΔ2-6 Other Phenotypes Allele cell shape defective HemdsRNA.cKa neuroanatomy defective HemG1-37 HemJ1-70 HemΔ2-6 neuroanatomy defective (with HemJ1-70) HemΔ2-6 neuroanatomy defective (with HemΔ2-6) HemJ1-70 neuroanatomy defective | dominant Hem03335 visible, with Scer\GAL4pnr-MD237 HemGD11783 wild-type, with Scer\GAL4rho.PL HemScer\UAS.T:Hsap\MYC Phenotype manifest in Allele actin filament HemdsRNA.cBa actin filament, with Scer\GAL4Act5C.PHb HemScer\UAS.T:Myr1 adult head & macrochaeta (with HemJ4-48) HemΔ2-6 adult head & macrochaeta (with HemΔ2-6) HemJ4-48 axon Hem03335 chaeta | supernumerary, with Scer\GAL4pnr-MD237 HemGD11783 commissure Hemunspecified cytoskeleton HemC3-20 Hemunspecified cytoskeleton & ectoderm Hemunspecified cytoskeleton & embryonic central nervous system Hemunspecified cytoskeleton & embryonic epidermis | dorsal HemC3-20 cytoskeleton & mesoderm Hemunspecified embryonic/larval midgut | embryonic stage Hemunspecified embryonic/larval neuromuscular junction (with HemJ4-48) HemΔ2-6 embryonic/larval neuromuscular junction (with HemΔ2-6) HemJ4-48 embryonic/larval somatic muscle HemG1-37 HemJ4-48 Hemunspecified embryonic myoblast HemJ4-48 interface glial cell Hemunspecified intersegmental nerve Hem03335 lamina plexus HemΔ2-6 larval neuromuscular junction & bouton, with Scer\GAL4elav-C155 HemScer\UAS.T:Myr1 larval neuromuscular junction & bouton (with HemJ4-48) HemΔ2-6 larval neuromuscular junction & bouton (with HemΔ2-6) HemJ4-48 longitudinal connective HemC3-20 HemJ1-70 HemJ4-48 Hemunspecified macrochaeta, with Scer\GAL4sca-537.4 HemScer\UAS.T:Myr1 medulla HemΔ2-6 microchaeta, with Scer\GAL4sca.PC HemScer\UAS.T:Myr1 microchaeta | supernumerary, with Scer\GAL4sca-537.4 HemScer\UAS.T:Myr1 midline glial cell HemC3-20 Hemunspecified motor neuron Hemunspecified muscle attachment site HemG1-37 neuromuscular junction & synapse Hem03335 HemJ1-70 neuromuscular junction & synapse (with HemJ1-70) HemΔ2-6 neuromuscular junction & synapse (with HemΔ2-6) HemJ1-70 photoreceptor cell & axon HemΔ2-6 presumptive embryonic/larval central nervous system Hemunspecified presumptive embryonic/larval peripheral nervous system Hemunspecified S2 cell-line & actin filament | ectopic HemdsRNA.cBb S2 cell-line & filopodium | ectopic HemdsRNA.cBb sensory neuron Hemunspecified sensory neuron | embryonic stage Hemunspecified somatic muscle primordium HemG1-37 HemJ4-48 thecogen cell & actin filament & pupa, with Scer\GAL4sca-537.4 HemScer\UAS.T:Myr1 thecogen cell | P-stage, with Scer\GAL4sca-537.4 HemScer\UAS.T:Myr1 ventral midline & neuron Hemunspecified ventral nerve cord commissure HemC3-20 HemJ1-70 HemJ4-48 VUM neuron Hemunspecified VUM neuron, with Scer\GAL4sim.PS HemScer\UAS.cHa
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of Hem Class Mutagen Stocks Known lesion HemC3-20 amorphic allele - genetic evidence ethyl methanesulfonate 1 Yes HemJ4-48 loss of function allele, amorphic allele - genetic evidence ethyl methanesulfonate 1 Yes Hem03335 hypomorphic allele - genetic evidence P-element activity 1 Yes HemΔ14-2 amorphic allele - genetic evidence P-element activity 0 Yes HemG1-37 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes HemJ1-70 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes HemP168 ethyl methanesulfonate 0 -- Hemunspecified 0 -- HemΔ2-6 hypomorphic allele - genetic evidence P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hem Class Mutagen Stocks Known lesion HemGD11783 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes HemJF03342 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes HemKK100336 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hem1-374.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Hem375-907.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Hem908-1126.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes HemdsRNA.cBa in vitro construct - RNAi 0 Yes HemdsRNA.cBb in vitro construct - RNAi 0 Yes HemdsRNA.cKa in vitro construct - RNAi 0 Yes HemScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes HemScer\UAS.cHa in vitro construct - regulatory fusion 0 Yes HemScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes HemScer\UAS.T:Myr1 in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)BSC420 (Christensen et al., 2008.4.15) Df(3L)BSC451 (Christensen et al., 2008.4.15) Df(3L)Exel6138 (Ryder, 2004.4.26)
Not disrupted in	Df(3L)Pc-MK (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11783} NA P{KK100336} NA P{TRiP.JF03342} NA P{UAS-Hem.1-374} NA P{UAS-Hem.375-907} NA P{UAS-Hem.908-1126} NA P{UAS-Hem.B} NA P{UAS-Hem.H} NA P{UAS-Hem.Myr} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}Hem03335 No
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Abi (Bogdan and Klambt, 2003)
Biological Process
CV term Evidence References
actin cytoskeleton organization inferred from mutant phenotype (Richardson et al., 2007) axonogenesis inferred from mutant phenotype (Hummel et al., 2000) cell morphogenesis inferred from mutant phenotype (D'Ambrosio and Vale, 2010) cell projection assembly inferred from mutant phenotype (Kunda et al., 2003) central nervous system development inferred from mutant phenotype (Hummel et al., 1999) chaeta morphogenesis inferred from mutant phenotype (Bogdan et al., 2004) cortical actin cytoskeleton organization inferred from mutant phenotype (Kunda et al., 2003) cytoskeleton organization inferred from mutant phenotype (Hummel et al., 2000) myoblast fusion inferred from mutant phenotype (Schroter et al., 2004, Richardson et al., 2007) neuromuscular junction development inferred from mutant phenotype (Bogdan et al., 2004) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) regulation of cell shape inferred from mutant phenotype (Kunda et al., 2003)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Khanna et al., 2010)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
oocyte growth non-traceable author statement (Swiss-Prot Project Members, 1996.10.1)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Strunk et al., 2003) plasma membrane non-traceable author statement (Baumgartner, 1994.7.1, Strunk et al., 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hem allele Gene References Hem03335 Fmr1 (Schenck et al., 2004) HemC3-20 SCAR (Bogdan and Klambt, 2003) HemdsRNA.cBa SCAR (Bogdan and Klambt, 2003) HemG1-37 blow (Schroter et al., 2004) HemJ4-48 Rac1 (Hummel et al., 2000) HemScer\UAS.cHa blow (Schroter et al., 2004) HemScer\UAS.T:Myr1 Sra-1 (Bogdan et al., 2004) WASp (Bogdan and Klambt, 2003) Hemunspecified Sra-1 (Bogdan et al., 2004) HemΔ2-6 dock (Hummel et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65698 DroID - A comprehensive database of gene and protein interactions. • FBgn0011771 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40462
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23466 Dere\GG13184 Dgri\GH14818 Dmoj\GI13924 Dper\GL20745 Dpse\GA19168 Dsec\GM22095 Dsim\Hem Dvir\GJ13713 Dwil\GK17072
Curated drosophilid orthologs	Dmir\CG5837-like   Dpse\GA19168   Dsim\Hem (Swanson et al., 2001) Dyak\Hem
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL009677-PA Aedes aegypti (mosquito) AGAP004946-PA Anopheles gambiae (mosquito) XP_624331.1 Apis mellifera (honey bee) At2g35110.1 Arabidopsis thaliana (wall cress) 92248 Batrachochytrium dendrobatidis (chytrid fungus) 123628 Branchiostoma floridae (lancelet) XP_001898481.1 Brugia malayi (roundworm nematode) BGIBMGA002428-PA Bombyx mori (silk worm) ENSBTAP00000044656 Bos taurus (bovine) CBP06483 Caenorhabditis briggsae (nematode) CE05750 Caenorhabditis elegans (worm) ENSCAFP00000021253 Canis familiaris (dog) ENSCINP00000006506 Ciona intestinalis (sea squirt) ENSCPOP00000010696 Cavia porcellus (guinea pig) RP27967 Caenorhabditis remanei (nematode) ENSCSAVP00000019298 Ciona savignyi (sedentary tunicate) 228503 Capitella spl (polychaete worm) DDB0231423 Dictyostelium discoideum (amoeba) 303127 Daphnia pulex (daphnia) ENSDARP00000080601 Danio rerio (zebra fish) ENSECAP00000019451 Equus caballus (horse) ENSGACP00000015379 Gymnopilus aculeatus (mushroom) ENSGALP00000004335 Gallus gallus (chicken) 101134 Hypsipyla robusta (cedar shoot borer) ENSP00000354251 Homo sapiens (human) ISCW009279-PA Ixodes scapularis (deer tick) 163399 Lottia gigantea (owl limpet) 37880 Monocosta brevicollis (choanoflagellate) ENSMODP00000013229 Monodelphis domestica (gray opossum) ENSMMUP00000018915 Macaca mulatta (rhesus monkey) ENSMUSP00000107390 Mus musculus (house mouse) 182004 Nematostella vectensis (starlet anemone) XP_001602104.1 Nasonia vitripennis (wasp) ENSOANP00000005750 Ornithorynchus anatinus (platypus) ENSORLP00000024309 Oryzias latipes (japanese killifish) LOC_Os08g43130.1 Oryza sativa (asian rice) PHUM005564-PA Pediculus humanus (human body louse) PP11715 Pristionchus pacificus (nematode) 180888 Physcomitrella patens (moss) ENSPPYP00000014519 Pongo pygmaeus (orangutan) 128694 Phytophthora sojae (root rot pathogen) 575639 Populus trichocarpa (black cottonwood) ENSPTRP00000045251 Pan troglodytes (chimpanzee) ENSRNOP00000010177 Rattus norvegicus (norway rat) 4765249 Sorghum bicolor (sorghum) Smp_001390 Schistosoma mansoni (trematode parasite) XP_001184421.1 Strongylocentrotus purpuratus (purple sea urchin) XP_971119.1 Tribolium castaneum (red flour beetle) ENSTNIP00000012750 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000008545 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000002843 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13879 LH21803 ISCW009279 FBgn0219071 FBgn0200934 FBgn0045567 FBgn0176966 JGI_V11_303127 FBgn0079165 FBgn0158340 FBgn0136678 FBgn0122294 FBgn0105456 FBgn0100460 CPIJ002287 BGIBMGA002428 GB13021 AGAP004946 AAEL012159 AAEL009677 PB21321 PHUM588610 GLEAN_01541
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	29406 y1 v1; P{TRiP.JF03342}attP2 8752 st1 e1 HemC3-20/TM6B, Tb1 8753 st1 e1 HemJ4-48/TM6B, P{ase-lacZF:2.0}PK3, Tb1 11584 P{PZ}Hem03335 ry506/TM3, ryRK Sb1 Ser1
VDRC	v103380 P{KK100336}VIE-260B v22207 w1118 P{GD11783}v22207
Genomic Clones ( 1 )
	BACR48E10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 73 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD43495
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD43480 RE30922 RE73923 SD03101 SD06426 SD17210 SD27712 SD27750
Other clones	28319 28438 61671 63537 82154 CK01.1A.C2 CK01.29C.G6 CK01.67B.E5 CK01.105D.F8 Dmel_neb_012864_0116_1811 EK026159 EK054119 EK088456 EK108603 EK135946 EK185063 EK190341 EK213238 ESG015d.E21.1_D11 RE22992 RP003006026 RP003010253 RP003037611 RP003054893 RP003056470 RP003062496 RP003098449 RP003104493 RP003106053 RP003107461 RP003139532 RP003146520 RP003161142 RP003165203 RP003187756 RP003189366 RP003196331 RP003204514 RP003208855 RP003216534 RP003232726 RP003269925 RP003279484 RP003292062 RP003296681 RP003321966 RP003343672 RP003344241 RP003345660 RP003384083 RP003388703 RP003390772 RP003422275 RP003422921 RP003516798 RP003527343 RP003552765 RP003554145 RP003554775 RP003583916 RP003588271 RP003590146 RP003624997 RP003647098
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011771 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40462
Antibody Information
	polyclonal (Gautier et al., 2011, Bogdan and Klambt, 2003) polyclonal antibody (Schenck et al., 2004)
Other Information
Discoverer
Etymology
	"kette" means "chain" in German. (Hummel et al., 1999)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Hem CG5837 (Worley, 1999.11)
Source for database merge of	Source for merge of: Hem kte (Hummel et al., 2000)
Additional comments
Other Comments
	In the context of myoblast fusion, Hem functions as a positive regulator of the localization and stability of SCAR. (Richardson et al., 2007) Hem antagonizes WASp function during myoblast fusion. (Schafer et al., 2007) S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) Hem is necessary for the second round of somatic myoblast fusion in the embryo. (Schroter et al., 2004) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) Hem is required for the normal projection of the VUM axons. Hem affects the organisation of the cytoskeleton. (Hummel et al., 2000) Five EMS induced alleles were identified in a screen for mutations affecting commissure formation in the CNS of the embryo. (Hummel et al., 1999) Identification: Part of molecular analysis of the Ten-m genomic region. (Baumgartner et al., 1995) Hem has been cloned and sequenced, and its expression pattern has been analysed. (Baumgartner et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079490 RefSeq (Proteins) • NP_524214 DDBJ / EMBL / GenBank • AAF51820 AAM49948 AI511922 AQ026185 AW943661 AY118579 CAA56332 X80028 Entrez Gene - A searchable database of RefSeq genes. • 40462 UniProtKB/Swiss-Prot • P55162
Other Crossreferences
Linkouts
	BioGRID - A database of protein and genetic interactions • 65698 DroID - A comprehensive database of gene and protein interactions. • FBgn0011771 DRSC - Results from RNAi screens. • FBgn0011771 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011771 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5837-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011771 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40462 InParanoid A subset of ortholog calls from InParanoid. • AAEL009677-PA Aedes aegypti (mosquito) AGAP004946-PA Anopheles gambiae (mosquito) XP_624331.1 Apis mellifera (honey bee) At2g35110.1 Arabidopsis thaliana (wall cress) 92248 Batrachochytrium dendrobatidis (chytrid fungus) 123628 Branchiostoma floridae (lancelet) XP_001898481.1 Brugia malayi (roundworm nematode) BGIBMGA002428-PA Bombyx mori (silk worm) ENSBTAP00000044656 Bos taurus (bovine) CBP06483 Caenorhabditis briggsae (nematode) CE05750 Caenorhabditis elegans (worm) ENSCAFP00000021253 Canis familiaris (dog) ENSCINP00000006506 Ciona intestinalis (sea squirt) ENSCPOP00000010696 Cavia porcellus (guinea pig) RP27967 Caenorhabditis remanei (nematode) ENSCSAVP00000019298 Ciona savignyi (sedentary tunicate) 228503 Capitella spl (polychaete worm) DDB0231423 Dictyostelium discoideum (amoeba) 303127 Daphnia pulex (daphnia) ENSDARP00000080601 Danio rerio (zebra fish) ENSECAP00000019451 Equus caballus (horse) ENSGACP00000015379 Gymnopilus aculeatus (mushroom) ENSGALP00000004335 Gallus gallus (chicken) 101134 Hypsipyla robusta (cedar shoot borer) ENSP00000354251 Homo sapiens (human) ISCW009279-PA Ixodes scapularis (deer tick) 163399 Lottia gigantea (owl limpet) 37880 Monocosta brevicollis (choanoflagellate) ENSMODP00000013229 Monodelphis domestica (gray opossum) ENSMMUP00000018915 Macaca mulatta (rhesus monkey) ENSMUSP00000107390 Mus musculus (house mouse) 182004 Nematostella vectensis (starlet anemone) XP_001602104.1 Nasonia vitripennis (wasp) ENSOANP00000005750 Ornithorynchus anatinus (platypus) ENSORLP00000024309 Oryzias latipes (japanese killifish) LOC_Os08g43130.1 Oryza sativa (asian rice) PHUM005564-PA Pediculus humanus (human body louse) PP11715 Pristionchus pacificus (nematode) 180888 Physcomitrella patens (moss) ENSPPYP00000014519 Pongo pygmaeus (orangutan) 128694 Phytophthora sojae (root rot pathogen) 575639 Populus trichocarpa (black cottonwood) ENSPTRP00000045251 Pan troglodytes (chimpanzee) ENSRNOP00000010177 Rattus norvegicus (norway rat) 4765249 Sorghum bicolor (sorghum) Smp_001390 Schistosoma mansoni (trematode parasite) XP_001184421.1 Strongylocentrotus purpuratus (purple sea urchin) XP_971119.1 Tribolium castaneum (red flour beetle) ENSTNIP00000012750 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000008545 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000002843 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/kette1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40462 modMine - Data generated by the modENCODE project. • FBgn0011771 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13879 LH21803 ISCW009279 FBgn0219071 FBgn0200934 FBgn0045567 FBgn0176966 JGI_V11_303127 FBgn0079165 FBgn0158340 FBgn0136678 FBgn0122294 FBgn0105456 FBgn0100460 CPIJ002287 BGIBMGA002428 GB13021 AGAP004946 AAEL012159 AAEL009677 PB21321 PHUM588610 GLEAN_01541
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	CG5837 (Rogers et al., 2003, Keleman et al., 2009.8.5, Perkins et al., 2009, Khanna et al., 2010) dhem (Baumgartner, 1994.7.1, ) dhem-2 (Baumgartner et al., 1995) Hem (Stroschein-Stevenson et al., 2006, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Xing et al., 2007, Kesper et al., 2007, Wasbrough et al., 2010) Hem1 (D'Ambrosio and Vale, 2010) kette (Chen and Olson, 2005, Schroter et al., 2004, Bogdan et al., 2004, Rogers et al., 2003, Wolfstetter et al., 2007, Richardson and Baylies, 2008, Chen and Chi, 2008, Kesper et al., 2007, Massarwa et al., 2007, Richardson et al., 2007, Lin et al., 2009, Gildor et al., 2009, Schafer et al., 2007, Eriksson et al., 2010, Gohl et al., 2010) Kette (Schenck et al., 2004, Bogdan et al., 2005, Schroeter et al., 2006, Chen et al., 2007, Massarwa et al., 2007) Kette/Nap1 (Gohl et al., 2010) kte (Granderath and Klambt, 1999, Hummel et al., 1999, Hummel et al., 1999, ) l(3)03335   Nap1 (Chen et al., 2007, Richardson and Baylies, 2008, Richardson et al., 2007)
Name Synonym	hemkette (Wolfstetter et al., 2007) HEM-protein (Xing et al., 2007, Khanna et al., 2010) kette (Richardson and Baylies, 2005, Chotard and Salecker, 2004, Vartiainen and Machesky, 2004, Smith and Li, 2004, Richardson and Baylies, 2004, Billuart and Chelly, 2003, Strunk et al., 2003, Baas and Luo, 2001, Pielage et al., 2001, Kunda et al., 2003, Bogdan and Klambt, 2003, Hummel et al., 1999, Hummel et al., 1999, Richardson et al., 2006, Richardson and Baylies, 2007, Li and Baker, 2007, Beckett and Baylies, 2007, Schafer et al., 2007, Jin et al., 2011) Kette (Rogers et al., 2003, Fricke et al., 2009, Bogdan et al., 2005, Qurashi et al., 2007)
Secondary FlyBase IDs
	FBgn0010794 FBgn0026230
References ( 71 )
Generate a list of	All references relating to this gene ( 71 )
List References by type	Research paper  ( 30 ) Supplementary material  ( 8 ) Review  ( 8 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 12 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 1 ) Computer file  ( 2 ) Protein sequence record  ( 1 )
Recent research papers ( 5 )
	Gautier et al., 2011, J. Cell Sci. 124(20): 3414--3427 Clathrin is required for Scar/Wave-mediated lamellipodium formation. [FBrf0216441] Jin et al., 2011, Dev. Cell 20(5): 623--638 Competition between Blown Fuse and WASP for WIP Binding Regulates the Dynamics of WASP-Dependent Actin Polymerization In Vivo. [FBrf0213682] D'Ambrosio and Vale, 2010, J. Cell Biol. 191(3): 471--478 A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis. [FBrf0212211] Eriksson et al., 2010, BMC Dev. Biol. 10: 86 Characterisation of the role of Vrp1 in cell fusion during the development of visceral muscle of Drosophila melanogaster. [FBrf0211723] Gohl et al., 2010, J. Biol. Chem. 285(51): 40171--40179 WAVE Forms Hetero- and Homo-oligomeric Complexes at Integrin Junctions in Drosophila Visualized by Bimolecular Fluorescence Complementation. [FBrf0212496] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...