kette, Nap1, kte, Kette/Nap1, dhem
alternatively a membrane associated and a cytosolic protein that transduces information to the neuronal cytoskeleton affecting axon guidance - Kette/Nap1/Hem-2 coordinates myoblast junction dissolution and the Scar-WASp ratio during myoblast fusion
Please see the JBrowse view of Dmel\Hem for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.45
3.8 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
1126 (aa); 129 (kD predicted)
Antibody was raised against the peptide RHNDNPPLLKNKGC
Component of the WAVE complex composed of Hem/Kette, Scar/Wave and Cyfip where it binds directly to the C-terminus of Cyfip.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hem using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
In Northern blots, Hem transcript is detected throughout development, with higher levels in the early embryo, in early first larval instar, and in adults. The expression detected in adults is likely due to ovarian accumulation. Hem transcript is observed in oogenesis and embryogenesis using in situ hybridization. In stage 10 oocytes, the follicle cell and the oocyte express Hem. Hem is ubiquitous in the early embryo. At embryonic stages 7-10, the presumptive procephalic neuroblasts express Hem. Expression is once again ubiquitous in stage 13 embryos. At stage 14, expression is strongest in the pharynx, supraoesophageal ganglion, and the ventral nerve cord. In stage 17 embryos, transcript is detected only in the brain and ventral cord. This pattern persists in the early larval stages.
Comment: expression assayed in cultured S2 cells
Hem protein is present in longitudinal connectives and commissures during the establishment of the axonal network.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Hem in JBrowsePlease Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
Hem is necessary for the second round of somatic myoblast fusion in the embryo.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
Five EMS induced alleles were identified in a screen for mutations affecting commissure formation in the CNS of the embryo.
Identification: Part of molecular analysis of the Ten-m genomic region.
Hem has been cloned and sequenced, and its expression pattern has been analysed.
Source for merge of: Hem kte
Source for identity of: Hem CG5837
"kette" means "chain" in German.
