Nlk, adk, Nemo-like kinase, adirondack
protein serine/threonine kinase involved in establishing ommatidial rotation/planar cell polarity, retinal specification and neuronal function, and circadian clock speed - regulates the activity of several signal transduction pathways, including BMP, Wnt, and Notch - implicated in fly models of neurodegenerative diseases
Please see the JBrowse view of Dmel\nmo for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Stop-codon suppression (UGA) postulated; FBrf0234051.
Gene model reviewed during 6.25
7.6, 7.2, 5.8, 5.4, 4.6, 4.1, 3.6, 3.2 (northern blot)
477, 434 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nmo using the Feature Mapper tool.
Comment: maternally deposited
Comment: zygotic expression in most cells
Comment: extended 3' UTR isoform
Comment: narrow stripe
Comment: 25-30 hr APF; expression is enriched immediately adjacent to wing veins
Isoforms of nmo with extended 3' UTRs appear later in embryogenesis. Sequential, phased, 3' UTR lengthening is seen (3' UTR lengthening ocurring in multiple steps during later stages of embryogenesis).
One nmo isoform is expressed in a stripe posterior the morphogenetic furrow. The other is expressed at low levels throughout the disc.
nmo transcripts are expressed in a complex pattern throughout development. Six transcripts are expressed in early embryos, (3.2, 3.6, 4.1, 4.6, 5.4, and 5.8 kb). A 7.2kb transcript appears in late embryos and a 7.6kb transcript is detected in adult heads. The seven transcripts are all developmentally regulated with varying patterns. In general, transcription is highest in embryos, third instar larvae, and pupae.
nmo transcripts are detected close to the morphogenetic furrow in third instar eye discs.
GBrowse - Visual display of RNA-Seq signals
View Dmel\nmo in GBrowse 23-21
3-18.6
3-66
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: nmo anon- EST:Gibbs3
Source for merge of: nmo anon-WO0118547.332 anon-WO0140519.256
Source for merge of nmo anon- EST:Gibbs3 was sequence comparison ( date:030707 ).
Source for merge of nmo anon-WO0118547.332 anon-WO0140519.256 was sequence comparison ( date:051113 ).
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, spindle abnormalities, centrosome abnormalities, lagging chromatids and a whole range of mitotic abnormalities are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Area matching Drosophila Nemo gene, Acc. No. U12010.
Area matching Drosophila EST AA439393 (inverted).
nmo is required during wing development for wing shape and vein patterning.
"Nemo" is Korean for a four-sided figure.