FlyBase Gene Report: Dmel\Pp2B-14D

General Information

Symbol

Dmel\Pp2B-14D

Species

D. melanogaster

Name

Protein phosphatase 2B at 14D

Annotation Symbol

CG9842

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011826

Gene Model Status

Stock Availability

7 publicly available

Enzyme Name (EC)

Protein-serine/threonine phosphatase (3.1.3.16)

Gene Snapshot

In progress.Contributions welcome.

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

CnnA14D, canA, calcineurin, PP2B 14D

Key Links

Genomic Location

Cytogenetic map

Sequence location

X:16,554,627..16,560,016 [-]

Recombination map

1-54

RefSeq locus

NC_004354 REGION:16554627..16560016

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

PHOSPHOPROTEIN PHOSPHATASES

Protein Family (UniProt)

Belongs to the PPP phosphatase family. PP-2B subfamily. (Q27889)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

calmodulin-dependent protein phosphatase activity; protein binding

Predictions / Assertions

calcium-dependent protein serine/threonine phosphatase activity; calmodulin binding; calmodulin-dependent protein phosphatase activity; protein serine/threonine phosphatase activity

Catalytic Activity (EC)

Experimental Evidence

-

Predictions / Assertions

[a protein]-serine/threonine phosphate + H(2)O = [a protein]- serine/threonine + phosphate (3.1.3.16)

Summaries

Gene Group (FlyBase)

PHOSPHOPROTEIN PHOSPHATASES -: The Phosphoprotein Phosphatase (PPP) family members are serine/threonine phosphatases. They often function in association with regulatory subunits. (Adapted from PMID:11230548 and PMID:19879837).

Protein Function (UniProtKB)

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

(UniProt, Q27889)

Summary (Interactive Fly)

Calcium-dependent protein serine/threonine phosphatase - negatively regulates Egf receptor signaling - calcineurin isoforms are involved in Drosophila toll immune signaling correcting calcineurin and GSK-3β signaling can prevent amyloid precursor protein-induced pathologies

Interactive Fly

Gene Model and Products

Number of Transcripts

3

Number of Unique Polypeptides

1

Please see the GBrowse view of Dmel\Pp2B-14D or the JBrowse view of Dmel\Pp2B-14D for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.46

Gene model reviewed during 5.41

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

protein_coding_gene

retrotransposed_protein_coding_gene

(dos Santos, 2014, Miskei et al., 2011, Bai et al., 2007, Langille and Clark, 2007)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0074294

3253

570

FBtr0305593

4551

570

FBtr0309793

5390

570

Additional Transcript Data and Comments

Reported size (kB)

4.5, 2.0 (northern blot)

(Hong and Ganetzky, 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0074069

63.1

570

5.73

FBpp0294044

63.1

570

5.73

FBpp0301546

63.1

570

5.73

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

570 aa isoforms: Pp2B-14D-PA, Pp2B-14D-PB, Pp2B-14D-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

578 (aa)

(Hong and Ganetzky, 1996)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with sra in a complex that contains CanA-14F.

(UniProt, Q27889)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pp2B-14D using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pp2B-14D_1

Linkouts

Gene Ontology (14 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

calmodulin-dependent protein phosphatase activity

inferred from mutant phenotype

(assigned by UniProt )

(Brown et al., 1994)

protein binding

inferred from physical interaction with FLYBASE:sra; FB:FBgn0086370

(Takeo et al., 2006)

inferred from physical interaction with UniProtKB:Q9XZL8

(assigned by UniProt )

(Takeo et al., 2012)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

calcium-dependent protein serine/threonine phosphatase activity

inferred from sequence or structural similarity

(Brown et al., 1994)

calmodulin binding

traceable author statement

(Montell, 1999)

inferred from biological aspect of ancestor with PANTHER:PTN000746105

(assigned by GO_Central )

(Gaudet et al., 2011)

calmodulin-dependent protein phosphatase activity

inferred from biological aspect of ancestor with PANTHER:PTN000746105

(assigned by GO_Central )

(Gaudet et al., 2011)

protein serine/threonine phosphatase activity

inferred from sequence or structural similarity

(Chen et al., 1992)

Biological Process (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

female meiotic nuclear division

inferred from mutant phenotype

(Takeo et al., 2006)

meiotic cell cycle

inferred from genetic interaction with FLYBASE:sra; FB:FBgn0086370

(Takeo et al., 2010)

regulation of embryonic development

inferred from mutant phenotype

(Takeo et al., 2010)

inferred from direct assay

(Nakai et al., 2011)

wing disc development

inferred from mutant phenotype

(Takeo et al., 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

calcineurin-mediated signaling

inferred from biological aspect of ancestor with PANTHER:PTN000746105

(assigned by GO_Central )

(Gaudet et al., 2011)

protein dephosphorylation

inferred from sequence or structural similarity

(Brown et al., 1994, Chen et al., 1992)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

calcineurin complex

inferred from biological aspect of ancestor with PANTHER:PTN000746105

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity

(Brown et al., 1994)

inferred from biological aspect of ancestor with PANTHER:PTN000746105

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval peripheral nervous system

(Hong and Ganetzky, 1996)

central nervous system

(Hong and Ganetzky, 1996)

embryonic stage 1 -- 5

(Hong and Ganetzky, 1996)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- adult stage

(Hong and Ganetzky, 1996)

Additional Descriptive Data

Pp2B-14D is expressed ubiquitously before gastrulation, but disappears after gastrulation. After the start of neurogenesis, expression is detected in the CNS and the PNS.

(Hong and Ganetzky, 1996)

Two and 4.5 kb Pp2B-14D transcripts were detected in larvae and adults.

(Hong and Ganetzky, 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pp2B-14D in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pp2B-14D

View exonic expression by tissue for Dmel\Pp2B-14D

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0011826

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9842-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9842

Flygut - An atlas of the Drosophila adult midgut

FBgn0011826

Images

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pp2B-14D

Transgenic constructs containing regulatory region of Pp2B-14D

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^how-24B

Pp2B-14D^act.UAS

lethal - all die during larval stage, with Scer\GAL4^Act5C.PI

Pp2B-14D^act.UAS.EYFP

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Pp2B-14D^act.UAS.EYFP

partially lethal - majority die | germline clone, with Scer\GAL4^VP16.nos.UTR

Pp2B-14D^DN.UASp

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pp2B-14D^GD17183

viable, with Scer\GAL4^Mef2.PR

Pp2B-14D^GD17183

viable, with Scer\GAL4^pnr-MD237

Pp2B-14D^GD17183

viable, with Scer\GAL4^tin.CΔ4

Pp2B-14D^GD17183

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

Pp2B-14D^HMS02039

female sterile, with Scer\GAL4^VP16.nos.UTR

Other Phenotypes

Allele

locomotor behavior defective

Pp2B-14D^KO

locomotor behavior defective, with Scer\GAL4^elav.PLu

Pp2B-14D^Act.UAS

meiotic cell cycle defective, with Scer\GAL4^VP16.nos.UTR

Pp2B-14D^{act.tin.C.EYFP}

size defective, with Scer\GAL4^en-e16E

Pp2B-14D^act.UAS

size defective | adult stage

size defective | adult stage, with Scer\GAL4^tin.CΔ4

Pp2B-14D^act.UAS.EYFP

sleep defective

Pp2B-14D^KO

sleep defective, with Scer\GAL4^elav.PLu

Pp2B-14D^Act.UAS

Pp2B-14D^act.GMR

visible, with Scer\GAL4^dpp.blk1

Pp2B-14D^act.UAS.EYFP

visible, with Scer\GAL4^en-e16E

visible, with Scer\GAL4^hs.2sev

Pp2B-14D^act.UAS

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^tin.CΔ4

Pp2B-14D^act.UAS.EYFP

adult heart muscle, with Scer\GAL4^tin.CΔ4

Pp2B-14D^act.UAS.EYFP

egg & nuclear chromosome, with Scer\GAL4^VP16.nos.UTR

Pp2B-14D^act.UASp

egg & spindle, with Scer\GAL4^VP16.nos.UTR

Pp2B-14D^act.UASp

Pp2B-14D^act.GMR

eye, with Scer\GAL4^GMR.PF

Pp2B-14D^act.UAS

eye photoreceptor cell, with Scer\GAL4^hs.2sev

Pp2B-14D^act.UAS

interommatidial bristle, with Scer\GAL4^GMR.PF

Pp2B-14D^act.UAS

Pp2B-14D^act.GMR

ommatidium, with Scer\GAL4^GMR.PF

Pp2B-14D^act.UAS

oocyte & nuclear chromatin, with Scer\GAL4^VP16.nos.UTR

Pp2B-14D^act.UASp

oocyte & nucleus, with Scer\GAL4^VP16.nos.UTR

Pp2B-14D^act.UASp

photoreceptor cell R3, with Scer\GAL4^hs.2sev

Pp2B-14D^act.UAS

photoreceptor cell R4, with Scer\GAL4^hs.2sev

Pp2B-14D^act.UAS

photoreceptor cell R7, with Scer\GAL4^hs.2sev

Pp2B-14D^act.UAS

posterior crossvein, with Scer\GAL4^en-e16E

Pp2B-14D^act.UAS.EYFP

wing, with Scer\GAL4^dpp.blk1

Pp2B-14D^act.UAS.EYFP

wing, with Scer\GAL4^en-e16E

wing blade, with Scer\GAL4^en-e16E

Pp2B-14D^act.UAS

wing blade posterior compartment, with Scer\GAL4^en-e16E

Pp2B-14D^act.UAS

wing vein, with Scer\GAL4^en-e16E

Pp2B-14D^act.UAS

wing vein L5, with Scer\GAL4^en-e16E

Pp2B-14D^act.UAS.EYFP

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

12 of 15

No

Yes

12 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

13 of 15

Yes

Yes

11 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Rattus norvegicus (Norway rat) (12)

10 of 13

Yes

Yes

9 of 13

No

Yes

8 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (12)

12 of 12

Yes

Yes

10 of 12

No

Yes

7 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Danio rerio (Zebrafish) (17)

10 of 15

Yes

Yes

10 of 15

Yes

Yes

10 of 15

Yes

Yes

8 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (44)

15 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Cele\Y71G12B.30

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (13)

12 of 15

Yes

Yes

12 of 15

Yes

Yes

2 of 15

No

No

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

11 of 12

Yes

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919060Y )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00008222

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila mojavensis

Drosophila grimshawi

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503T8 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Musca domestica

House fly

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY008907

Glossina morsitans

Tsetse fly

Gmor\GMOY008078

Glossina morsitans

Tsetse fly

Gmor\GMOY007597

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10408

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11822

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_02720

Mayetiola destructor

Hessian fly

Mdes\Mdes002974

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008930

Anopheles darlingi

American malaria mosquito

Adar\ADAC008703

Anopheles gambiae

Malaria mosquito

Agam\AGAP001279

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018692

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03LH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Danaus plexippus

Monarch butterfly

Dple\DPOGS206069-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL005036

Apis florea

Little honeybee

Aflo\2201296148

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0011e2

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007022

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007393

Dendroctonus ponderosae

Mountain pine beetle

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM596230

Rhodnius prolixus

Kissing bug

Rpro\RPRC009380

Cimex lectularius

Bed bug

Clec\CLEC001543

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003343

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08435

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03IC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Black-legged tick

Isca\ISCW024405

Stegodyphus mimosarum

African social velvet spider

Smim\KFM63371.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM80850.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g07540

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g07560

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_62512

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_312053

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G094R )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\LOC100185597

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (18)

Gene Symbol

Score

Source

Alignment

9 of 10

8 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

cardiomyopathy (postulated), calcineurin-A-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

Pp2B-14D^{act.tin.C.EYFP}

model of cardiomyopathy

CEA

(Lee et al., 2014)

Pp2B-14D^{act.tin.C.Tag:FLAG}

model of cardiomyopathy

CEA

(Lee et al., 2014)

Pp2B-14D^act.UAS.EYFP

model of cardiomyopathy

CEA

(Lee et al., 2014)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PPP3CA; protein phosphatase 3 catalytic subunit alpha

model of infantile or early childhood epileptic encephalopathy 1

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 4 )

Allele

Disease

Interaction

References

Pp2B-14D^Act.Δ.UAS

exacerbates Alzheimer's disease

modeled by Hsap\APP^{695.UAS.Tag:MYC}

(Shaw and Chang, 2013)

Pp2B-14D^{act.tin.C.EYFP}

model of cardiomyopathy

is ameliorated by Galk^MB10638

(Lee et al., 2014)

is ameliorated by Galk^KK107841

(Lee et al., 2014)

is ameliorated by galectin^DG25505

(Lee et al., 2014)

Pp2B-14D^{act.tin.C.Tag:FLAG}

model of cardiomyopathy

is ameliorated by Mef2^X1

(Lee et al., 2014)

Pp2B-14D^act.UAS.EYFP

model of cardiomyopathy

is ameliorated by sty^Δ5

(Lee et al., 2014)

is ameliorated by Mef2^X1

(Lee et al., 2014)

is ameliorated by CanB2^EP774

(Lee et al., 2014)

is ameliorated by Galk^MB10638

(Lee et al., 2014)

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PPP3CA; protein phosphatase 3 catalytic subunit alpha

14 of 15

infantile or early childhood epileptic encephalopathy 1

PPP3CB; protein phosphatase 3 catalytic subunit beta

12 of 15

PPP3CC; protein phosphatase 3 catalytic subunit gamma

12 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pp2B-14D - CG15446

(Hu et al., 2017.6.13)

Pp2B-14D - CG9578

(Hu et al., 2017.6.13)

Pp2B-14D - CanA1

experimental knowledge based

(Guruharsha et al., 2011)

Pp2B-14D - Hsp67Bc

(Hu et al., 2017.6.13)

Pp2B-14D - Nf-YA

(Hu et al., 2017.6.13)

Pp2B-14D - Pi3K21B

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Pp2B-14D - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, anti tag western blot

(Li and Dijkers, 2015)

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag western blot, multidimensional protein identification technology

(Takeo et al., 2012, Takeo et al., 2010, Takeo et al., 2006)

Pp2B-14D - tamo

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lee et al., 2014, Takeo et al., 2010, Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

enhanceable

(Wang et al., 2008)

suppressible

(Wang et al., 2008)

suppressible

(Sullivan and Rubin, 2002)

suppressible

(Sullivan and Rubin, 2002)

suppressible

(Sullivan and Rubin, 2002)

suppressible

(Sullivan and Rubin, 2002)

suppressible

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

suppressible

(Lee et al., 2014)

suppressible

(Lee et al., 2014)

suppressible

(Sullivan and Rubin, 2002)

suppressible

(Lee et al., 2014)

enhanceable

(Sullivan and Rubin, 2002)

suppressible

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

suppressible

(Sullivan and Rubin, 2002)

suppressible

(Sullivan and Rubin, 2002)

suppressible

(Shaw and Chang, 2013)

suppressible

(Sullivan and Rubin, 2002)

suppressible

(Sullivan and Rubin, 2002)

suppressible

(Takeo et al., 2010, Takeo et al., 2006)

enhanceable

(Nakai et al., 2011, Takeo et al., 2010)

suppressible

(Lee et al., 2014, Sullivan and Rubin, 2002)

suppressible

(Sullivan and Rubin, 2002)

enhanceable

(Sullivan and Rubin, 2002)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Shaw and Chang, 2013, Nakai et al., 2011)

External Data

Subunit Structure (UniProtKB)

Interacts with sra in a complex that contains CanA-14F.

(UniProt, Q27889 )

Linkouts

BioGRID - A database of protein and genetic interactions.

58962

DroID - A comprehensive database of gene and protein interactions.

FBgn0011826

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32624

MIST (genetic) - An integrated Molecular Interaction Database

Pp2B-14D

MIST (protein-protein) - An integrated Molecular Interaction Database

Pp2B-14D

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Wnt signaling pathway

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

X

Recombination map

1-54

Cytogenetic map

Sequence location

X:16,554,627..16,560,016 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

14E1-14E3

Limits computationally determined from genome sequence between P{EP}EP1547^EP1547 and P{EP}CG4928^EP1341

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

14D-14D

(Hong, 1995.6.28)

14D1-14D4

(Brown, 1994.9.22)

14D1-14D4

(determined by in situ hybridisation)

(Brown et al., 1994)

Experimentally Determined Recombination Data

Location

1-54

(FlyBase, 2016)

1-55.5

(Comeron et al., 2012)

1-

(Hong, 1995.6.28)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

25929

y¹ v¹; P{TRiP.JF01949}attP2

80331

y¹ v¹; P{TKO.GS03291}attP40

22025

w^67c23 P{Mae-UAS.6.11}Pp2B-14D^GG01028

40872

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02039}attP2

v103144

P{KK107714}VIE-260B

v46873

w¹¹¹⁸; P{GD17183}v46873

v342281

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01983}attP40/CyO-GFP

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (33)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD04578

Other clones

DPiM_LD04578

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011826

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011826

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32624

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Pp2B-14D PpD33

(FlyBase, 1996-)

Additional comments

The Pp2B-14D gene may have been derived from a related phosphoprotein phosphatase gene by retroposition and subsequent tandem duplication.

(Miskei et al., 2011)

The Pp2B-14D gene may have been derived from the CanA1 gene by retroposition.

(Bai et al., 2007, Langille and Clark, 2007)

Source for merge of Pp2B-14D PpD33 was sequence comparison ( date:050803 ).

(FlyBase, 1996-)

Other Comments

The region that includes para contains a complex of neurally expressed genes, Cnx14D, Arp14D, Pp2B-14D and Cap. These five genes may represent a functionally related group sharing some coordinate regulatory mechanism.

(Hong and Ganetzky, 1996)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 55 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32624

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q27889

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011826

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pp2B-14D_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9842

Flygut - An atlas of the Drosophila adult midgut

FBgn0011826

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32624

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000103

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG9842

modMine - A data warehouse for the modENCODE project

FBgn0011826

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q27889

Linkouts

BioGRID - A database of protein and genetic interactions.

58962

DPiM - Drosophila Protein interaction map

FBgn0011826

DroID - A comprehensive database of gene and protein interactions.

FBgn0011826

DRSC - Results frm RNAi screens

FBgn0011826

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0011826

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9842-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011826

FlyMine - An integrated database for Drosophila genomics

FBgn0011826

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32624

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Wnt signaling pathway

MIST (genetic) - An integrated Molecular Interaction Database

Pp2B-14D

MIST (protein-protein) - An integrated Molecular Interaction Database

Pp2B-14D

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (26)

Reported As

Symbol Synonym

CG9842

(Neuert et al., 2017, Çiçek et al., 2016, Hosono et al., 2015, Kanamori et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Wheeler et al., 2004, Morrison et al., 2000, Verstreken and Bellen, 2000.9.4)

CaN

(Vonhoff and Keshishian, 2017)

CaNA

(Shaw and Chang, 2013)

CanA

(Lee et al., 2014)

CanA14D

CnA

(Takeo et al., 2010)

CnnA14D

(Hong and Ganetzky, 1996, Hong, 1995.6.28, Hong, 1995.6.29)

D33

(Chen et al., 1992)

DmPp2B-14D

(Cohen et al., 2005)

PP2B

(Helps et al., 1998)

PP2B 14D

(Morrison et al., 2000, Cohen, 1997, Cohen, 1994.5.16)

PP2B-14D

(Ha et al., 2009)

Pp12-14D

(Whitfield, 2000.5.8)

Pp2B

(Zarndt et al., 2017)

Pp2B-14D/CaN

(Yin et al., 2013)

Pp2B-14DF

Pp2b-14D

(Ray et al., 2016)

PpD33

calneur

(Bodaleo and Gonzalez-Billault, 2016)

canA

(Sullivan and Rubin, 2002, Sullivan and Rubin, 2001, Sullivan and Rubin, 2001)

Name Synonyms

Calcineurin

(Vonhoff and Keshishian, 2017, Dijkers and O'Farrell, 2007, Xu et al., 1998)

Protein phosphatase 2B at 14D

(John et al., 2016)

Protein phosphatase D33

calcineurin

(Bodaleo and Gonzalez-Billault, 2016, Dijkers and O'Farrell, 2007, Montell, 1999)

protein phosphatase from PCR fragment D33

(Chen, 1993.1.7)

Secondary FlyBase IDs

FBgn0005782
FBgn0005783
FBgn0015613

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (97)

Report Sections