FlyBase Gene Report: Dmel\Pxn

posterior midgut primordium

(Nelson et al., 1994)

embryonic stage 9 -- 10

crystal cell primordium

Comment: reported as crystal cell specific anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

garland cell primordium

(Fisher et al., 2012)

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

(Fisher et al., 2012)

procrystal cell

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval crystal cell

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic/larval garland cell

(Fisher et al., 2012)

embryonic/larval lymph gland

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

larval stage

(Nelson et al., 1994)

gastric caecum

(Nelson et al., 1994)

Additional Descriptive Data

Pxn is transcribed primarily in hemocytes and later in cells of the fat body. Transcription starts in the two plates of the procephalic mesoderm at stage 10. Expression in the hemocytes continues as the hemocyte population expands and spreads through the embryo until at later stages Pxn transcripts are found at sites of basement membrane formation, e.g. around the gut, nerve cord, and brain.

(Nelson et al., 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

• basement membrane

(Nelson et al., 1994)

(Nelson et al., 1994)

embryonic stage 12 -- 17

(Heino et al., 2001)

embryonic stage 13 -- 15

(Paladi and Tepass, 2004)

embryonic stage 16

cortical zone of lymph gland primary lobe

(Yin et al., 1997)

fat body primordium

(Yin et al., 1997)

ring gland

(Yin et al., 1997)

larval stage

(Mondal et al., 2014, Sinenko et al., 2009)

wandering third instar larval stage

(Sinenko et al., 2009)

primary lobe of embryonic/larval lymph gland | restricted

(Dragojlovic-Munther and Martinez-Agosto, 2012)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

cortical zone of lymph gland primary lobe

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Early differentiating hemocytes express both the prohemocyte marker dome and low levels of the early differentiation marker Pxn. These cells represent a population of intermediate progenitors. As they mature, differentiating hemocytes lose dome and upregulate Pxn.

(Dragojlovic-Munther and Martinez-Agosto, 2012)

Pxn is an early maturation marker in hemocytes.

(Sinenko et al., 2009)

Pxn protein is localized to hemocytes. It is initially found in the two plates of the procephalic mesoderm at stage 10. Expression in the hemocytes continues as the hemocyte population expands and spreads through the embryo until at later stages Pxn protein is found at sites of basement membrane formation, e.g. around the gut, nerve cord, and brain.

(Nelson et al., 1994)

Marker for

(Mondal et al., 2014, Sinenko et al., 2009)

(Heino et al., 2001, Yin et al., 1997)

wandering third instar larval stage

(Paladi and Tepass, 2004)

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.2}Pxn^{MI01492-TG4.2}

Stage

Tissue/Position (including subcellular localization)

Reference

larval central nervous system | restricted

(Lee et al., 2018)

ventral nerve cord | restricted

(Lee et al., 2018)

adult mushroom body | restricted

adult brain | restricted

(Lee et al., 2018)

(Lee et al., 2018)

Reporter: P{Pxn-GAL4}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Stramer et al., 2005)

embryonic stage 10 -- 17

(Verboon et al., 2015)

embryonic stage 12 -- 15

(Wood et al., 2006)

embryonic stage 14 -- adult stage

(Shia et al., 2009)

embryonic stage 15 -- 17

(Wood et al., 2006)

larval stage

cortical zone of lymph gland primary lobe

(Shirinian et al., 2015, Khadilkar et al., 2014, Brock et al., 2012)

(Khadilkar et al., 2014, Mondal et al., 2014)

(Han et al., 2014, Honti et al., 2010)

pupal stage

(Shimono et al., 2009)

(Chen et al., 2014, Zaidman-Remy et al., 2006)

Reporter: PBac{802.P.SVS-2}Pxn^CPTI003897

Stage

Tissue/Position (including subcellular localization)

Reference