The gene TBP-associated factor 2 is referred to in FlyBase by the symbol Dmel\Taf2 (CG6711, FBgn0011836). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly; transcription factor binding; sequence-specific DNA binding; sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly. There is experimental evidence that it is involved in the biological process: transcription from RNA polymerase II promoter; neurogenesis. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Peptidase M1, membrane alanine aminopeptidase, N-terminal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1629974_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Taf2. Gene sequence location is 3L:9877511..9881781.
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modENCODE Temporal Expression Data for FBgn0011836
Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages.
[download data (TSV)]
Guide to modENCODE expression level colors
No expression (0 - 0)
Extremely low expression (1 - 10)
Very low expression (11 - 100)
Low expression (101 - 400)
Moderate expression (401 - 1400)
Moderately high expression (1401 - 4000)
High expression (4001 - 10000)
Very high expression (10001 - 100000)
Extremely high expression (100001 - 2000000)
Linear, scaled to maximum FBgn0011836 expression level
Developmental Stage
Expression Level
embryo 00-02hr
2930
embryo 02-04hr
4205
embryo 04-06hr
2837
embryo 06-08hr
2475
embryo 08-10hr
2503
embryo 10-12hr
1648
embryo 12-14hr
1416
embryo 14-16hr
1145
embryo 16-18hr
803
embryo 18-20hr
392
embryo 20-22hr
674
embryo 22-24hr
691
larva L1
489
larva L2
543
larva L3 12hr old
459
larva L3 puffstage 1-2
546
larva L3 puffstage 3-6
773
larva L3 puffstage 7-9
856
white prepupae new
907
white prepupae 12hr
981
white prepupae 24hr
1317
pupae 2d postWPP
665
pupae 3d postWPP
358
pupae 4d postWPP
215
adult male 01day
342
adult male 05day
455
adult male 30day
426
adult female 01day
851
adult female 05day
1524
adult female 30day
1581
Expression Level Scale
None
Extremely low
Very low
Low
Moderate
Moderately high
High
Linear, scaled to Very low expression
Developmental Stage
Expression Level
embryo 00-02hr
(2930)
embryo 02-04hr
(4205)
embryo 04-06hr
(2837)
embryo 06-08hr
(2475)
embryo 08-10hr
(2503)
embryo 10-12hr
(1648)
embryo 12-14hr
(1416)
embryo 14-16hr
(1145)
embryo 16-18hr
(803)
embryo 18-20hr
(392)
embryo 20-22hr
(674)
embryo 22-24hr
(691)
larva L1
(489)
larva L2
(543)
larva L3 12hr old
(459)
larva L3 puffstage 1-2
(546)
larva L3 puffstage 3-6
(773)
larva L3 puffstage 7-9
(856)
white prepupae new
(907)
white prepupae 12hr
(981)
white prepupae 24hr
(1317)
pupae 2d postWPP
(665)
pupae 3d postWPP
(358)
pupae 4d postWPP
(215)
adult male 01day
(342)
adult male 05day
(455)
adult male 30day
(426)
adult female 01day
(851)
adult female 05day
(1524)
adult female 30day
(1581)
Expression Level Scale
None
Extremely low
Very low
Low
Linear, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(2930)
embryo 02-04hr
(4205)
embryo 04-06hr
(2837)
embryo 06-08hr
(2475)
embryo 08-10hr
(2503)
embryo 10-12hr
(1648)
embryo 12-14hr
1416
embryo 14-16hr
1145
embryo 16-18hr
803
embryo 18-20hr
392
embryo 20-22hr
674
embryo 22-24hr
691
larva L1
489
larva L2
543
larva L3 12hr old
459
larva L3 puffstage 1-2
546
larva L3 puffstage 3-6
773
larva L3 puffstage 7-9
856
white prepupae new
907
white prepupae 12hr
981
white prepupae 24hr
1317
pupae 2d postWPP
665
pupae 3d postWPP
358
pupae 4d postWPP
215
adult male 01day
342
adult male 05day
455
adult male 30day
426
adult female 01day
851
adult female 05day
(1524)
adult female 30day
(1581)
Expression Level Scale
None
Extremely low
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
2930
embryo 02-04hr
4205
embryo 04-06hr
2837
embryo 06-08hr
2475
embryo 08-10hr
2503
embryo 10-12hr
1648
embryo 12-14hr
1416
embryo 14-16hr
1145
embryo 16-18hr
803
embryo 18-20hr
392
embryo 20-22hr
674
embryo 22-24hr
691
larva L1
489
larva L2
543
larva L3 12hr old
459
larva L3 puffstage 1-2
546
larva L3 puffstage 3-6
773
larva L3 puffstage 7-9
856
white prepupae new
907
white prepupae 12hr
981
white prepupae 24hr
1317
pupae 2d postWPP
665
pupae 3d postWPP
358
pupae 4d postWPP
215
adult male 01day
342
adult male 05day
455
adult male 30day
426
adult female 01day
851
adult female 05day
1524
adult female 30day
1581
Expression Level Scale
None
Extremely low
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
2930
embryo 02-04hr
4205
embryo 04-06hr
2837
embryo 06-08hr
2475
embryo 08-10hr
2503
embryo 10-12hr
1648
embryo 12-14hr
1416
embryo 14-16hr
1145
embryo 16-18hr
803
embryo 18-20hr
392
embryo 20-22hr
674
embryo 22-24hr
691
larva L1
489
larva L2
543
larva L3 12hr old
459
larva L3 puffstage 1-2
546
larva L3 puffstage 3-6
773
larva L3 puffstage 7-9
856
white prepupae new
907
white prepupae 12hr
981
white prepupae 24hr
1317
pupae 2d postWPP
665
pupae 3d postWPP
358
pupae 4d postWPP
215
adult male 01day
342
adult male 05day
455
adult male 30day
426
adult female 01day
851
adult female 05day
1524
adult female 30day
1581
Expression Level Scale
None
Extremely low
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
log, scaled to maximum FBgn0011836 expression level
Developmental Stage
Expression Level
embryo 00-02hr
2930
embryo 02-04hr
4205
embryo 04-06hr
2837
embryo 06-08hr
2475
embryo 08-10hr
2503
embryo 10-12hr
1648
embryo 12-14hr
1416
embryo 14-16hr
1145
embryo 16-18hr
803
embryo 18-20hr
392
embryo 20-22hr
674
embryo 22-24hr
691
larva L1
489
larva L2
543
larva L3 12hr old
459
larva L3 puffstage 1-2
546
larva L3 puffstage 3-6
773
larva L3 puffstage 7-9
856
white prepupae new
907
white prepupae 12hr
981
white prepupae 24hr
1317
pupae 2d postWPP
665
pupae 3d postWPP
358
pupae 4d postWPP
215
adult male 01day
342
adult male 05day
455
adult male 30day
426
adult female 01day
851
adult female 05day
1524
adult female 30day
1581
Expression Level Scale
None
Extremely low
Very low
Low
Moderate
Moderately high
High
log, scaled to Very low expression
Developmental Stage
Expression Level
embryo 00-02hr
(2930)
embryo 02-04hr
(4205)
embryo 04-06hr
(2837)
embryo 06-08hr
(2475)
embryo 08-10hr
(2503)
embryo 10-12hr
(1648)
embryo 12-14hr
(1416)
embryo 14-16hr
(1145)
embryo 16-18hr
(803)
embryo 18-20hr
(392)
embryo 20-22hr
(674)
embryo 22-24hr
(691)
larva L1
(489)
larva L2
(543)
larva L3 12hr old
(459)
larva L3 puffstage 1-2
(546)
larva L3 puffstage 3-6
(773)
larva L3 puffstage 7-9
(856)
white prepupae new
(907)
white prepupae 12hr
(981)
white prepupae 24hr
(1317)
pupae 2d postWPP
(665)
pupae 3d postWPP
(358)
pupae 4d postWPP
(215)
adult male 01day
(342)
adult male 05day
(455)
adult male 30day
(426)
adult female 01day
(851)
adult female 05day
(1524)
adult female 30day
(1581)
Expression Level Scale
None
Extremely low
Very low
Low
log, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(2930)
embryo 02-04hr
(4205)
embryo 04-06hr
(2837)
embryo 06-08hr
(2475)
embryo 08-10hr
(2503)
embryo 10-12hr
1648
embryo 12-14hr
1416
embryo 14-16hr
1145
embryo 16-18hr
803
embryo 18-20hr
392
embryo 20-22hr
674
embryo 22-24hr
691
larva L1
489
larva L2
543
larva L3 12hr old
459
larva L3 puffstage 1-2
546
larva L3 puffstage 3-6
773
larva L3 puffstage 7-9
856
white prepupae new
907
white prepupae 12hr
981
white prepupae 24hr
1317
pupae 2d postWPP
665
pupae 3d postWPP
358
pupae 4d postWPP
215
adult male 01day
342
adult male 05day
455
adult male 30day
426
adult female 01day
851
adult female 05day
1524
adult female 30day
1581
Expression Level Scale
None
Extremely low
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
2930
embryo 02-04hr
4205
embryo 04-06hr
2837
embryo 06-08hr
2475
embryo 08-10hr
2503
embryo 10-12hr
1648
embryo 12-14hr
1416
embryo 14-16hr
1145
embryo 16-18hr
803
embryo 18-20hr
392
embryo 20-22hr
674
embryo 22-24hr
691
larva L1
489
larva L2
543
larva L3 12hr old
459
larva L3 puffstage 1-2
546
larva L3 puffstage 3-6
773
larva L3 puffstage 7-9
856
white prepupae new
907
white prepupae 12hr
981
white prepupae 24hr
1317
pupae 2d postWPP
665
pupae 3d postWPP
358
pupae 4d postWPP
215
adult male 01day
342
adult male 05day
455
adult male 30day
426
adult female 01day
851
adult female 05day
1524
adult female 30day
1581
Expression Level Scale
None
Extremely low
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
2930
embryo 02-04hr
4205
embryo 04-06hr
2837
embryo 06-08hr
2475
embryo 08-10hr
2503
embryo 10-12hr
1648
embryo 12-14hr
1416
embryo 14-16hr
1145
embryo 16-18hr
803
embryo 18-20hr
392
embryo 20-22hr
674
embryo 22-24hr
691
larva L1
489
larva L2
543
larva L3 12hr old
459
larva L3 puffstage 1-2
546
larva L3 puffstage 3-6
773
larva L3 puffstage 7-9
856
white prepupae new
907
white prepupae 12hr
981
white prepupae 24hr
1317
pupae 2d postWPP
665
pupae 3d postWPP
358
pupae 4d postWPP
215
adult male 01day
342
adult male 05day
455
adult male 30day
426
adult female 01day
851
adult female 05day
1524
adult female 30day
1581
Expression Level Scale
None
Extremely low
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Developmental Stage
Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day
FlyAtlas Anatomical Expression Data for FBgn0011836
Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, adult ovary.
[download data (TSV)]
Guide to FlyAtlas expression level colors
No expression (0 - 9.999)
Low expression (10 - 99.999)
Moderate expression (100 - 499.999)
High level expression (500 - 999.999)
Very high expression (1000 - 25000)
Linear, scaled to maximum FBgn0011836 expression level
Tissue
Expression Level
Larval Central Nervous System
124.775
Larval Midgut
24.9
Larval Hindgut
50.5
Larval Malpighian Tubules
37
Larval Fat Body
43.4
Larval Salivary Gland
106.3
Larval Trachea
85.625
Larval Carcass
52.85
Adult Head
43.6
Adult Eye
25.7
Adult Brain
64.4
Adult Thoracic-Abdominal Ganglion
57.3
Adult Crop
54.7
Adult Midgut
no informative data
Adult Hindgut
51.7
Adult Malpighian Tubules
52.4
Adult Fat Body
no informative data
Adult Salivary Gland
no informative data
Adult Heart
34.5
Adult VirginFemale Spermatheca
40.3
Adult InseminatedFemale Spermatheca
53.2
Adult Ovary
147
Adult Testis
65
Adult Male Accessory Gland
79.5
Adult Carcass
no informative data
Expression Level Scale
None
Low
Moderate
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
124.775
Larval Midgut
24.9
Larval Hindgut
50.5
Larval Malpighian Tubules
37
Larval Fat Body
43.4
Larval Salivary Gland
106.3
Larval Trachea
85.625
Larval Carcass
52.85
Adult Head
43.6
Adult Eye
25.7
Adult Brain
64.4
Adult Thoracic-Abdominal Ganglion
57.3
Adult Crop
54.7
Adult Midgut
no informative data
Adult Hindgut
51.7
Adult Malpighian Tubules
52.4
Adult Fat Body
no informative data
Adult Salivary Gland
no informative data
Adult Heart
34.5
Adult VirginFemale Spermatheca
40.3
Adult InseminatedFemale Spermatheca
53.2
Adult Ovary
147
Adult Testis
65
Adult Male Accessory Gland
79.5
Adult Carcass
no informative data
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
124.775
Larval Midgut
24.9
Larval Hindgut
50.5
Larval Malpighian Tubules
37
Larval Fat Body
43.4
Larval Salivary Gland
106.3
Larval Trachea
85.625
Larval Carcass
52.85
Adult Head
43.6
Adult Eye
25.7
Adult Brain
64.4
Adult Thoracic-Abdominal Ganglion
57.3
Adult Crop
54.7
Adult Midgut
no informative data
Adult Hindgut
51.7
Adult Malpighian Tubules
52.4
Adult Fat Body
no informative data
Adult Salivary Gland
no informative data
Adult Heart
34.5
Adult VirginFemale Spermatheca
40.3
Adult InseminatedFemale Spermatheca
53.2
Adult Ovary
147
Adult Testis
65
Adult Male Accessory Gland
79.5
Adult Carcass
no informative data
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
124.775
Larval Midgut
24.9
Larval Hindgut
50.5
Larval Malpighian Tubules
37
Larval Fat Body
43.4
Larval Salivary Gland
106.3
Larval Trachea
85.625
Larval Carcass
52.85
Adult Head
43.6
Adult Eye
25.7
Adult Brain
64.4
Adult Thoracic-Abdominal Ganglion
57.3
Adult Crop
54.7
Adult Midgut
no informative data
Adult Hindgut
51.7
Adult Malpighian Tubules
52.4
Adult Fat Body
no informative data
Adult Salivary Gland
no informative data
Adult Heart
34.5
Adult VirginFemale Spermatheca
40.3
Adult InseminatedFemale Spermatheca
53.2
Adult Ovary
147
Adult Testis
65
Adult Male Accessory Gland
79.5
Adult Carcass
no informative data
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to maximum FBgn0011836 expression level
Tissue
Expression Level
Larval Central Nervous System
124.775
Larval Midgut
24.9
Larval Hindgut
50.5
Larval Malpighian Tubules
37
Larval Fat Body
43.4
Larval Salivary Gland
106.3
Larval Trachea
85.625
Larval Carcass
52.85
Adult Head
43.6
Adult Eye
25.7
Adult Brain
64.4
Adult Thoracic-Abdominal Ganglion
57.3
Adult Crop
54.7
Adult Midgut
no informative data
Adult Hindgut
51.7
Adult Malpighian Tubules
52.4
Adult Fat Body
no informative data
Adult Salivary Gland
no informative data
Adult Heart
34.5
Adult VirginFemale Spermatheca
40.3
Adult InseminatedFemale Spermatheca
53.2
Adult Ovary
147
Adult Testis
65
Adult Male Accessory Gland
79.5
Adult Carcass
no informative data
Expression Level Scale
None
Low
Moderate
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
124.775
Larval Midgut
24.9
Larval Hindgut
50.5
Larval Malpighian Tubules
37
Larval Fat Body
43.4
Larval Salivary Gland
106.3
Larval Trachea
85.625
Larval Carcass
52.85
Adult Head
43.6
Adult Eye
25.7
Adult Brain
64.4
Adult Thoracic-Abdominal Ganglion
57.3
Adult Crop
54.7
Adult Midgut
no informative data
Adult Hindgut
51.7
Adult Malpighian Tubules
52.4
Adult Fat Body
no informative data
Adult Salivary Gland
no informative data
Adult Heart
34.5
Adult VirginFemale Spermatheca
40.3
Adult InseminatedFemale Spermatheca
53.2
Adult Ovary
147
Adult Testis
65
Adult Male Accessory Gland
79.5
Adult Carcass
no informative data
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
124.775
Larval Midgut
24.9
Larval Hindgut
50.5
Larval Malpighian Tubules
37
Larval Fat Body
43.4
Larval Salivary Gland
106.3
Larval Trachea
85.625
Larval Carcass
52.85
Adult Head
43.6
Adult Eye
25.7
Adult Brain
64.4
Adult Thoracic-Abdominal Ganglion
57.3
Adult Crop
54.7
Adult Midgut
no informative data
Adult Hindgut
51.7
Adult Malpighian Tubules
52.4
Adult Fat Body
no informative data
Adult Salivary Gland
no informative data
Adult Heart
34.5
Adult VirginFemale Spermatheca
40.3
Adult InseminatedFemale Spermatheca
53.2
Adult Ovary
147
Adult Testis
65
Adult Male Accessory Gland
79.5
Adult Carcass
no informative data
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
124.775
Larval Midgut
24.9
Larval Hindgut
50.5
Larval Malpighian Tubules
37
Larval Fat Body
43.4
Larval Salivary Gland
106.3
Larval Trachea
85.625
Larval Carcass
52.85
Adult Head
43.6
Adult Eye
25.7
Adult Brain
64.4
Adult Thoracic-Abdominal Ganglion
57.3
Adult Crop
54.7
Adult Midgut
no informative data
Adult Hindgut
51.7
Adult Malpighian Tubules
52.4
Adult Fat Body
no informative data
Adult Salivary Gland
no informative data
Adult Heart
34.5
Adult VirginFemale Spermatheca
40.3
Adult InseminatedFemale Spermatheca
53.2
Adult Ovary
147
Adult Testis
65
Adult Male Accessory Gland
79.5
Adult Carcass
no informative data
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
no informative data
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
no informative data
Adult Salivary Gland
no informative data
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
no informative data
FlyAtlas Organ/Tissue Expression, larval vs. adult
Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
cDNA Clones ( 110 )
Please Note
This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see GBrowse for alignment of the cDNAs and ESTs
to the gene model.
The tandem Adh promoters are differentially transcribed in the embryo owing to critical differences in the core promoter elements. Reconstituted differential Adh promoter transcription in vitro provides evidence that selective Adh promoter utilization is mediated by a specific Tbp-TAF complex in combination with TfIIA. TAFs in the Tbp complex are required for discrimination between the Adh distal and proximal initiator elements.
The assembly and purification of various functional Tbp TAF complexes demonstrate that Tbp plus the TAFs is sufficient to replace endogenous TFIID to support activated transcription in vitro.