FlyBase Gene Report: Dmel\Cdc27

General Information

Symbol

Dmel\Cdc27

Species

D. melanogaster

Name

Cell division cycle 27

Annotation Symbol

CG8610

Feature Type

protein_coding_gene

FlyBase ID

FBgn0012058

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Cell division cycle 27 (Cdc27) encodes a component of the anaphase-promoting complex/cyclosome (APC/C). It is important for substrate recruitment and interacts with its co-activators, encoded by fzy and fzr, as well as the mitotic checkpoint proteins, encoded by mad2 and BubR1. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

mks, makos, APC3, l(3)L7123

Key Links

Genomic Location

Cytogenetic map

65E9-65E9

Sequence location

3L:7,245,481..7,249,340 [-]

Recombination map

3-19

RefSeq locus

NT_037436 REGION:7245481..7249340

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in anaphase-promoting complex-dependent catabolic process

inferred from mutant phenotype

(Huang et al., 2007)

involved_in mitotic cell cycle

inferred from high throughput mutant phenotype

(Ducat et al., 2008)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in anaphase-promoting complex-dependent catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000285286

(GO Reference Genome Project, 2011-)

involved_in cell division

inferred from biological aspect of ancestor with PANTHER:PTN001757014

(GO Reference Genome Project, 2011-)

involved_in metaphase/anaphase transition of mitotic cell cycle

inferred from biological aspect of ancestor with PANTHER:PTN000285286

(GO Reference Genome Project, 2011-)

involved_in mRNA splicing, via spliceosome

inferred by curator from GO:0071011

(Herold et al., 2009)

involved_in protein ubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN000285286

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

part_of anaphase-promoting complex

inferred from physical interaction with FLYBASE:APC7; FB:FBgn0029879

(Pal et al., 2007)

colocalizes_with condensed chromosome

inferred from direct assay

(Huang et al., 2007)

located_in nucleus

inferred from direct assay

(Huang et al., 2007)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of anaphase-promoting complex

inferred from biological aspect of ancestor with PANTHER:PTN000285286

(GO Reference Genome Project, 2011-)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000285286

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ANAPHASE-PROMOTING COMPLEX

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

ANAPHASE-PROMOTING COMPLEX -: Anaphase-promoting complex/cyclosome (APC/C) is a multisubunit E3 ubiquitin ligase. Active during mitosis and G1 phases of the cell cycle, it mediates the degradation of cyclins and other key cell cycle regulators triggering the metaphase to anaphase transition and the exit from mitosis. (Adapted from PMID:12208841).

Summary (Interactive Fly)

subunit of anaphase-promoting complex (APC) - involved in regulating cell cycle - important for substrate recruitment and interacts with its co-activators, Fizzy and Fizzy-related, as well as the mitotic checkpoint proteins, Mad2 and BubR1

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Cdc27 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VS37)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.40

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cdc27-RA

FBtr0076890

NM_139836

3438

900

Cdc27-RB

FBtr0333369

NM_001274574

3713

900

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Cdc27-PA

FBpp0076600

101.3

900

7.45

FBpp0305561

101.3

900

7.45

Q9VS37

NP_001261503

AGB94198

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

900 aa isoforms: Cdc27-PA, Cdc27-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cdc27 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cdc27_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.46

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of anaphase-promoting complex

inferred from physical interaction with FLYBASE:APC7; FB:FBgn0029879

(Pal et al., 2007)

colocalizes_with condensed chromosome

inferred from direct assay

(Huang et al., 2007)

located_in nucleus

inferred from direct assay

(Huang et al., 2007)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Cdc27 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cdc27

View exonic expression by tissue for Dmel\Cdc27

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0012058

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38798/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0012058

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0012058

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0012058

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8610

Flygut - An atlas of the Drosophila adult midgut

FBgn0012058

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cdc27

Transgenic constructs containing regulatory region of Cdc27

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel6111

(Ryder, 2004.4.26)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

Cdc27^GD13986

lethal, with Scer\GAL4^nub-AC-62

Cdc27^HMC03814

lethal | female limited, with Scer\GAL4^dome-PG14

Cdc27^HMC03814

lethal | pharate adult stage | recessive

lethal | recessive

lethal - all die before end of P-stage | recessive

Cdc27^S092309

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Cdc27^GD13986

partially lethal - majority die | female limited

partially lethal - majority die | recessive

Cdc27^L7123

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Cdc27^GD13986

viable, with Scer\GAL4^tin.CΔ4

Cdc27^GD13986

Sterility

Allele

female semi-sterile

female sterile

Cdc27^L7123

female sterile, with Scer\GAL4^VP16.otu

Cdc27^HMC03814

sterile | recessive

Cdc27^L7123

Other Phenotypes

Allele

abnormal flight, with Scer\GAL4^Mef2.PR

Cdc27^GD13986

abnormal mitotic cell cycle

Cdc27^S092309

abnormal mitotic cell cycle | larval stage

abnormal mitotic cell cycle | somatic clone | third instar larval stage

Cdc27^E45

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Cdc27^GD13986

abnormal size | adult stage, with Scer\GAL4^ey.PU

decreased cell number | somatic clone | third instar larval stage

Cdc27^E45

short lived

Cdc27^L7123

some die during pharate adult stage | recessive

Cdc27^S092309

some die during pupal stage, with Scer\GAL4^pnr-MD237

Cdc27^GD13986

visible, with Scer\GAL4^Bx-MS1096

Cdc27^HM04024

visible, with Scer\GAL4^pnr-MD237

Cdc27^GD13986

visible | adult stage, with Scer\GAL4^ey.PU

Cdc27^HM04024

visible | adult stage, with Scer\GAL4^GMR.PU

Cdc27^HM04024

visible | recessive

Cdc27^L7123

Phenotype manifest in

Allele

aster & neuroblast

Cdc27^L7123

chaeta

Cdc27^L7123

chromatin

Cdc27^S092309

chromatin & neuroblast

Cdc27^L7123

chromosome

Cdc27^S092309

chromosome & neuroblast

Cdc27^L7123

chromosome | increased number

Cdc27^L7123

condensed chromosome & neuroblast & larva

Cdc27^S092309

eye

Cdc27^L7123

eye, with Scer\GAL4^ey.PU

Cdc27^HM04024

eye, with Scer\GAL4^GMR.PU

Cdc27^HM04024

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Cdc27^GD13986

larval brain | somatic clone | third instar larval stage

Cdc27^E45

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Cdc27^GD13986

larval neuroblast | somatic clone | third instar larval stage

Cdc27^E45

mesonotum, with Scer\GAL4^pnr-MD237

Cdc27^GD13986

mitosis & nuclear chromosome

Cdc27^S092309

mitotic cell cycle

Cdc27^S092309

mitotic metaphase

Cdc27^S092309

mitotic metaphase & neuroblast

Cdc27^L7123

retina, with Scer\GAL4^ey.PU

spindle & neuroblast

Cdc27^L7123

spindle & neuroblast & larva

Cdc27^S092309

wing, with Scer\GAL4^Bx-MS1096

Cdc27^HM04024

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\CDC27

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (15)

Rnor\Cdc27

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (15)

Mmus\Cdc27

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (15)

Xtro\cdc27

11 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ttc37

1 of 13

Danio rerio (Zebrafish) (15)

Drer\cdc27

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\mat-1

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP007690

11 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (14)

Atha\HBT

13 of 13

Yes

Atha\CDC27a

12 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT2G32450

1 of 13

Atha\AT3G18420

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (8)

Scer\CDC27

10 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\nuc2

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (2)

Ecol\lapB

1 of 11

Yes

Ecol\yphG

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Cdc27. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (9)

3 of 13

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

sxc

1 of 13

Tmtc4

1 of 13

Tom70

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Cdc27^HM04024

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

ameliorates cancer

modeled by Pten^HMS00044, Ras85D^G13Q.UAS, Rbf^HMS03004

(Parkhitko et al., 2021)

Cdc27^HMC03814

ameliorates cancer

modeled by Pten^HMS00044, Ras85D^G13Q.UAS, Rbf^HMS03004

(Parkhitko et al., 2021)

Cdc27^02-055

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cJa

(Ren et al., 2022)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CDC27; cell division cycle 27

14 of 14

116946

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Cdc27 - APC7

affinity chromatography technology, western blot

(Pal et al., 2007)

Cdc27 - BubR1

anti bait coimmunoprecipitation, western blot

(Conde et al., 2013)

Cdc27 - Cdc16

anti tag coimmunoprecipitation, western blot

(Huang et al., 2007)

Cdc27 - Cdk1

anti tag coimmunoprecipitation, western blot

(Garrido et al., 2020)

Cdc27 - CycB3

anti tag coimmunoprecipitation, anti tag western blot

(Garrido et al., 2020)

Cdc27 - cort

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Whitfield et al., 2013, Pesin and Orr-Weaver, 2007)

Cdc27 - fzr

anti tag coimmunoprecipitation, peptide massfingerprinting

(Meghini et al., 2016)

Cdc27 - spd-2

anti tag coimmunoprecipitation, western blot

(Meghini et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Cdc27

enhanceable

polo

(Deak et al., 2003)

Cdc27

enhanceable

tws

(Deak et al., 2003)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

polo

enhanceable

Cdc27

(Deak et al., 2003)

tws

suppressible

Cdc27

(Deak et al., 2003)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64241

DroID - A comprehensive database of gene and protein interactions.

FBgn0012058

MIST (genetic) - An integrated Molecular Interaction Database

38798

MIST (protein-protein) - An integrated Molecular Interaction Database

38798

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ubiquitin mediated proteolysis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-19

Cytogenetic map

65E9-65E9

Sequence location

3L:7,245,481..7,249,340 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65E9-65E9

Limits computationally determined from genome sequence between P{PZ}corn⁰⁴²⁰² and P{lacW}Cdc27^L7123

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

65E7-65E12

(determined by in situ hybridisation)

(Deak et al., 2003)

65E-65E

(determined by in situ hybridisation)

(Deak, 1999.9.15)

65F1-65F2

(determined by in situ hybridisation)

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

65E7-65E12

(Deak and Glover, 1999)

Experimentally Determined Recombination Data

Location

3-19

(FlyBase, 2016)

3-16.9

(Comeron et al., 2012)

(Deak and Glover, 1999)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

31716

y¹ v¹; P{TRiP.HM04024}attP2

81686

y¹ v¹; P{TKO.GS03292}attP40

10168

y¹ w¹¹¹⁸; P{lacW}Cdc27^L7123/TM3, Ser¹

55155

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03814}attP40

FlyORF

F001053

M{UAS-Cdc27.ORF.3xHA}ZH-86Fb

Kyoto

207373

y¹ w^67c23; P{GSV2}Cdc27^GS51529/TM3, Sb¹ Ser¹

111012

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Cdc27^L7123 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹

VDRC

v35986

w¹¹¹⁸; P{GD13986}v35986

v35985

w¹¹¹⁸; P{GD13986}v35985/CyO

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (24)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD12661

Other clones

DPiM_LD12661

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0012058

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0012058

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis.

(Kiger et al., 2003)

"65E (determined by in situ hybridisation)" was stated as revision.

(Deak, 1999.9.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: Cdc27 l(3)L7123

(Spradling et al., 1999)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

APC3

(Garrido et al., 2020, Martins et al., 2017, Buttitta et al., 2010)

Apc3

(Pal et al., 2007, Tanaka-Matakatsu et al., 2007)

CDC27

(Péan and Dionne, 2014, Joyce et al., 2012, Akimana et al., 2010, Acquaviva and Pines, 2006)

CDC27Dm

(Tugendreich, 1994.12.7)

CG8610

(Neuert et al., 2017, Çiçek et al., 2016, Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2010.12.1, Herold et al., 2009, Ducat et al., 2008, Ni et al., 2008)

Dmcdc27

(Huang and Raff, 1999)

cdc27

(Henarejos-Castillo et al., 2024, Lee et al., 2017, Guest et al., 2011, Buttitta et al., 2010, Silies and Klämbt, 2010, Griffis et al., 2007, Huang et al., 2007, Sakaguchi and Steward, 2007, Slack et al., 2007, Lopes et al., 2005, Pal et al., 2005, Harper et al., 2002, Huang and Raff, 2002)

l(3)L7123

(BDGP Project Members, 1994-1999)

mks

(Pal et al., 2007, Tanaka-Matakatsu et al., 2007, Deak et al., 2003, Deak, 1999.9.15, Deak and Glover, 1999)

Name Synonyms

Cdc27

(Pesin and Orr-Weaver, 2007, Xing et al., 2007)

Cell division cycle 27

Cell division cycle 27 ortholog

Makos

(Pal et al., 2007)

makos

(Slack et al., 2006, Deak, 1999.9.15, Deak and Glover, 1999)

Secondary FlyBase IDs

FBgn0024570

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 56 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38798

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VS37

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VS37

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0012058

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38798/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0012058

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0012058

FlyMine - An integrated database for Drosophila genomics

FBgn0012058

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8610

MARRVEL_MODEL - MARRVEL (model organism gene)

38798

Linkouts

BioGRID - A database of protein and genetic interactions.

64241

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0012058

DroID - A comprehensive database of gene and protein interactions.

FBgn0012058

DRSC - Results frm RNAi screens

FBgn0012058

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cdc27_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0012058

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0012058

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8610

Flygut - An atlas of the Drosophila adult midgut

FBgn0012058

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0012058

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ubiquitin mediated proteolysis

MIST (genetic) - An integrated Molecular Interaction Database

38798

MIST (protein-protein) - An integrated Molecular Interaction Database

38798

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (97)

The following external sites may use different assemblies or annotations than FlyBase.

3.46

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