Gene Dleb\Adh

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General Information

Symbol

Dleb\Adh

Species

S. lebanonensis

Name

Alcohol dehydrogenase

Annotation symbol

Feature type

gene

FlyBase ID

FBgn0012438

Gene Model Status

Unannotated

Stock availability

None publicly available

Also Known As

ADH

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

Sequence location

Summary Information

Automatically generated summary

See sections below for more information

The gene Alcohol dehydrogenase is referred to in FlyBase by the symbol Dleb\Adh (FBgn0012438). It is a gene from Drosophila lebanonensis. Its molecular function is unknown. The biological processes in which it is involved are not known. 5 alleles are reported. No phenotypic data is available. It has no annotated transcripts. Gene has not been localized to the genome sequence.

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Additional Polypeptide Data & Comments

Reported size (kDa)

254 (aa)

(Villarroya et al., 1989)

Comments

The Adh gene product from D. lebanonensis has a 254 residue protein chain with an acetyl blocked N terminal Met.

(Villarroya et al., 1989)

External Data

Linkouts

Crossreferences

PDB - Protein Data Bank. An information portal to biological macromolecular structures

•

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

modENCODE Anatomy RNA-Seq

modENCODE Development RNA-Seq

modENCODE Cell Lines RNA-Seq

modENCODE Treatments RNA-Seq

Expression Clusters

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Dleb\Adh	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Dleb\Adh	Mutagen	Known lesion
Dleb\Adh^-1264	in vitro construct	Yes
Dleb\Adh^-2358	in vitro construct	Yes
Dleb\Adh^-6431	in vitro construct	Yes
Dleb\Adh^-830	in vitro construct	Yes
Dleb\Adh^-93	in vitro construct	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 0 terms )

Sequence Ontology: Class of Gene

gene

gene_with_dicistronic_mRNA

(Betran and Ashburner, 2000)

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Dleb\Adh allele

Gene

References

External Data

Linkouts

Orthologs

OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

No orthologs identified

Dipteran inclusive ortholog search

No orthologs identified

Insect inclusive ortholog search

No orthologs identified

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

No orthologs identified

Ortholog(s) in Drosophila melanogaster (None identified)

No D. melanogaster orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (0) based on analysis using Dmel annotation version 4.3

No orthologs identified

Stocks & Reagents

Stocks Listed in FlyBase ( 0 )

Genomic Clones ( 0 )

cDNA Clones ( 0 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

Dleb\Adhr is transcribed as a dicistronic mRNA with Dleb\Adh.

(Betran and Ashburner, 2000)

The crystal structure of Dleb\Adh protein has been determined.

(Benach et al., 1998)

The phylogenetic relationships and divergence times of 39 drosophilid species have been studied by using the coding region of the Adh gene.

(Russo et al., 1995)

The homologous genomic region containing Adh and Adhr is analysed. Ka and Ks values are determined (Ks values for Adh are significantly lower than values for Adhr) and amino acid comparisons reveal conserved regions shared by Adh and Adhr which have been assigned to known functional domains.

(Albalat et al., 1994)

The D.lebanonensis genomic region containing Dleb\Adh and Dleb\Adhr has been cloned and sequenced.

(Juan et al., 1994)

Adh activity in 71 Drosophila species is assayed to determine if the protein plays a key role in the adaptation of species to substrates undergoing alcoholic fermentation.

(Mercot et al., 1994)

The Adh genomic region of D.lebanonensis, which includes Dleb\Adh and Dleb\Adhr, has been cloned and sequenced, and compared with the corresponding region from D.immigrans and D.subobscura.

(Albalat and Gonzalez-Duarte, 1993)

Adult acetic acid tolerance found to correlate with ethanol tolerance.

(Chakir et al., 1993)

Phylogenetic DNA sequence comparison of ten Drosophila species has been used to elucidate the secondary structure of Adh mRNA. One possible pairing region in the 5' leader sequence and 22 in the coding and noncoding regions have been identified by seeking an equivalent pairing region in homologous RNA. Most are short range pairings such as hairpins. The rate of coevolution in noncoding pairing region is higher than in coding regions in accordance with selective constraints on substitution rates in coding region.

(Stephan and Kirby, 1993)

Phylogenetic relationships in Drosophila are studied using the Alcohol dehydrogenase locus in several species.

(Thomas and Hunt, 1993)

The functional Dleb\Adh gene product is a dimer with a monomeric Mr of 27000 and with 254 residues in each polypeptide chain. Crystals of the protein in the presence of cofactor are plate-like with varying dimensions and twinned. In the absence of cofactor crystals were monoclinic.

(Gordon et al., 1992)

Dleb\Adh amino acid sequence was compared to already known Drosophila Adh genes and 10 unique amino acid replacements were found. 4 nonconservative substitutions were found at positions 33, 456, 60 and 191. At position 191 a thr is present where it is a lys in all other Drosophila dehydrogenases, this substitution is also seen in Adh^F. 6 conservative substitutions, with respect to size and hydrophobicity, are present. At position 13, in the NAD binding domain, ala not gly is present as seen in other Drosophila dehydrogenases. 62.6% amino acid identity throughout Drosophila ADH proteins. Distance matrix and parsimony methods were used to establish the phylogenetic relationship of D.lebanonensis and demonstrated that the 3 subgenera Scaptodosophila, Drosophila and Sophophora separated at approximately the same time.

(Villarroya and Juan, 1991)

In situ hybridization assays reveal a single chromosomal location of Dleb\Adh.

(Visa et al., 1991)

Dleb\Adh coding region has been sequenced.

(Albalat et al., 1990)

Organization of Dleb\Adh locus has been determined.

(Juan et al., 1990)

Three Adh protein antibodies were characterized and immunoblotting assays found that they cross-react to Adh genes from D.melanogaster, D.bocqueti, D.erecta, D.teissieri and D.lebanonensis. ADH specific activity in different larval organs was found to be similar whereas protein distribution varies substantially.

(Fibla et al., 1989)

External Crossreferences & Linkouts

Sequence Crossreferences

DDBJ / EMBL / GenBank

•

Other Crossreferences

PDB - Protein Data Bank. An information portal to biological macromolecular structures

•

Linkouts

Synonyms & Secondary IDs ( 7 )

Reported As

Symbol Synonym

ADH

(Dorit and Ayala, 1995)

Adh

(LANG et al., 2007, PÃ©rez-Farrerons and Juan, 2007, Katoh et al., 2000, Wuxiuer et al., 2012, de Setta et al., 2007, Lang and Juan, 2010)

ADH-leb

(Benach et al., 2000)

DADH

(Benach et al., 1999)

Dleb\Adh

Name Synonym

Alcohol dehydrogenase

(Moriyama and Hartl, 1993)

alcohol dehydrogenase

(Katoh et al., 2000)

Secondary FlyBase IDs

References ( 54 )

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All references relating to this gene ( 54 )

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Research paper ( 47 )
Review ( 1 )
Erratum ( 1 )
Abstract ( 5 )

Recent research papers ( 1 )

Wuxiuer et al., 2012, FEBS J. 279(16): 2940--2956: An intact eight-membered water chain in drosophilid alcohol dehydrogenases is essential for optimal enzyme activity. [FBrf0218983]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2011

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