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Gene Dmel\Hsp70Ba

General Information
Symbol	Dmel\Hsp70Ba	Species	D. melanogaster
Name	Heat-shock-protein-70Ba	Annotation symbol	CG31449
Feature type	protein_coding_gene	FlyBase ID	FBgn0013277
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	Hsp70, hsp70B, dhsp70, hsp-70
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	87B12-87B12	Sequence location	3R:8,291,175..8,293,498 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heat-shock-protein-70Ba is referred to in FlyBase by the symbol Dmel\Hsp70Ba (CG31449, FBgn0013277). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: response to hypoxia; heat shock-mediated polytene chromosome puffing; response to heat. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Heat shock protein 70 family; Heat shock protein 70, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: No FlyAtlas data available because no Affy2 ProbeSet aligns to an exon of Hsp70Ba. Comments on Affy2 ProbeSet: No relevant information because no Affy2 ProbeSet aligns to an exon of Hsp70Ba. Gene sequence location is 3R:8291175..8293498.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Hsp70 The structural genes that code for the 70,000 dalton heat-shock protein (HSP70), the most abundant of the heat-shock proteins. HSP70 returns to preshock levels more rapidly than other heat-shock proteins following return to 25 (DiDomenico, Bugaisky, and Lindquist, 1982, Proc. Nat. Acad. Sci. USA 79: 6181-85). The protein becomes concentrated in nuclei during heat shock; disperses to cytoplasm during recovery; returns to nucleus upon further heat shock (Velazquez and Lindquist, 1984, Cell 36: 655-62). Appears not to be expressed in the testis in response to heat-shock stimulation (Bonner, Parks, Parker-Thornberg, Mortin, and Pelham, 1984, Cell 37: 979-91). Deletion of either the 87A7 or the 87C1 sequences does not eliminate the HSP70 heat-shock response; simultaneous deletion of both sequences does eliminate the HSP70 heat-shock response (Ish-Horowitz et al., 1979).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87B12-87B12   Limits computationally determined from genome sequence between P{PZ}svp07842 and P{lacW}Vha55j2E9
Experimentally Determined Cytological Location
Cytogenetic map Notes References 87C1-87C1   (determined by in situ hybridisation)   (Ranz et al., 1997) 87C-87C   (determined by in situ hybridisation)   (Segarra et al., 1996) 87C-87C   (determined by in situ hybridisation)   (Papaceit and Juan, 1993) 87C1-87C1   (determined by in situ hybridisation)   (Lis et al., 1981) 87C1-87C1   (determined by in situ hybridisation)   (Ish-Horowicz et al., 1979) 87C1-87C1   (determined by in situ hybridisation)   (Schedl et al., 1978)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hsp70Ba for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hsp70Ba-RA FBtr0082679  NM_169469   2324   641
Additional Transcript Data & Comments
Reported size (kB)	2.55 (northern blot) (Holmgren et al., 1979)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hsp70Ba-PA   FBpp0082147   70.2   641   5.49     NP_731716   AAN13545
Additional Polypeptide Data & Comments
Reported size (kDa)	641 (aa); 70.27 (kD predicted) (Ingolia et al., 1980) 70 (kD) (Holmgren et al., 1979)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Heat shock protein 70, conserved site (IPR018181) Heat shock protein 70 family (IPR013126)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name A15908   AA801760   AE014297 AAN13545   AF295946 AAG26900   AF295947 AAG26901   AF295948 AAG26902   AF295949 AAG26903   AF295950 AAG26904   AF350468 AAK30225   AF350469 AAK30226   AF350470 AAK30227   AF350471 AAK30228   AF350472 AAK30229   AF350473 AAK30230   AF350474 AAK30231   AF350475 AAK30232   AF377341 AAK54833   AF377953 AAK54836   AF385405 AAK67154   AF385406 AAK67155   AF385407 AAK67156   AF385408 AAK67157   AI260606   AI404714   AK256004   AX093917   AY032740 AAK62472   AY094878   AY118457   BF489797   BI633747   BT011058   BT011379   BT011541   BT021339   BT031349   BT099626   BT126332   BT126347   BX029450   CK604959   CK658400   CK659424   CK661232   CO188710   DQ093386   DQ093387   EC054721   EC080640   EC253197   EV595020   GH818584   K01293   K01294   M10178   V00219 CAA23500
UniProtKB/Swiss-Prot	Q8INI8
UniProtKB/TrEMBL	Q95NM1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    75 central nervous system, larvae L3    1 central nervous system, pupae P8    5 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    10 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    5 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    9 digestive system, 4-day adult    15 digestive system, 20-day adult    11 fat body, larvae L3 wandering    17 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    6 carcass, 1-day adult    30 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    0 ovary, mated 4-day female    1 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (75) central nervous system, larvae L3    1 central nervous system, pupae P8    5 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    10 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    5 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    9 digestive system, 4-day adult    15 digestive system, 20-day adult    11 fat body, larvae L3 wandering    17 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    6 carcass, 1-day adult  (30) carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    0 ovary, mated 4-day female    1 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    75 central nervous system, larvae L3    1 central nervous system, pupae P8    5 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    10 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    5 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    9 digestive system, 4-day adult    15 digestive system, 20-day adult    11 fat body, larvae L3 wandering    17 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    6 carcass, 1-day adult    30 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    0 ovary, mated 4-day female    1 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    75 central nervous system, larvae L3    1 central nervous system, pupae P8    5 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    10 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    5 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    9 digestive system, 4-day adult    15 digestive system, 20-day adult    11 fat body, larvae L3 wandering    17 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    6 carcass, 1-day adult    30 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    0 ovary, mated 4-day female    1 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    75 central nervous system, larvae L3    1 central nervous system, pupae P8    5 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    10 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    5 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    9 digestive system, 4-day adult    15 digestive system, 20-day adult    11 fat body, larvae L3 wandering    17 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    6 carcass, 1-day adult    30 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    0 ovary, mated 4-day female    1 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (75) central nervous system, larvae L3    1 central nervous system, pupae P8    5 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    10 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    5 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    9 digestive system, 4-day adult    15 digestive system, 20-day adult    11 fat body, larvae L3 wandering    17 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    6 carcass, 1-day adult  30 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    0 ovary, mated 4-day female    1 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    75 central nervous system, larvae L3    1 central nervous system, pupae P8    5 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    10 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    5 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    9 digestive system, 4-day adult    15 digestive system, 20-day adult    11 fat body, larvae L3 wandering    17 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    6 carcass, 1-day adult    30 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    0 ovary, mated 4-day female    1 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    75 central nervous system, larvae L3    1 central nervous system, pupae P8    5 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    10 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    5 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    9 digestive system, 4-day adult    15 digestive system, 20-day adult    11 fat body, larvae L3 wandering    17 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    6 carcass, 1-day adult    30 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    0 ovary, mated 4-day female    1 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    4 embryo 10-12hr    18 embryo 12-14hr    5 embryo 14-16hr    1 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    2 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    34 white prepupae 24hr    18 pupae 2d postWPP    26 pupae 3d postWPP    5 pupae 4d postWPP    33 adult male 01day    1 adult male 05day    1 adult male 30day    0 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    4 embryo 10-12hr    18 embryo 12-14hr    5 embryo 14-16hr    1 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    2 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr  (34) white prepupae 24hr    18 pupae 2d postWPP  26 pupae 3d postWPP    5 pupae 4d postWPP  (33) adult male 01day    1 adult male 05day    1 adult male 30day    0 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    4 embryo 10-12hr    18 embryo 12-14hr    5 embryo 14-16hr    1 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    2 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    34 white prepupae 24hr    18 pupae 2d postWPP    26 pupae 3d postWPP    5 pupae 4d postWPP    33 adult male 01day    1 adult male 05day    1 adult male 30day    0 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    4 embryo 10-12hr    18 embryo 12-14hr    5 embryo 14-16hr    1 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    2 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    34 white prepupae 24hr    18 pupae 2d postWPP    26 pupae 3d postWPP    5 pupae 4d postWPP    33 adult male 01day    1 adult male 05day    1 adult male 30day    0 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    4 embryo 10-12hr    18 embryo 12-14hr    5 embryo 14-16hr    1 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    2 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    34 white prepupae 24hr    18 pupae 2d postWPP    26 pupae 3d postWPP    5 pupae 4d postWPP    33 adult male 01day    1 adult male 05day    1 adult male 30day    0 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    4 embryo 10-12hr    18 embryo 12-14hr    5 embryo 14-16hr    1 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    2 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr  (34) white prepupae 24hr    18 pupae 2d postWPP  26 pupae 3d postWPP    5 pupae 4d postWPP  (33) adult male 01day    1 adult male 05day    1 adult male 30day    0 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    4 embryo 10-12hr    18 embryo 12-14hr    5 embryo 14-16hr    1 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    2 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    34 white prepupae 24hr    18 pupae 2d postWPP    26 pupae 3d postWPP    5 pupae 4d postWPP    33 adult male 01day    1 adult male 05day    1 adult male 30day    0 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    4 embryo 10-12hr    18 embryo 12-14hr    5 embryo 14-16hr    1 embryo 16-18hr    0 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    2 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    34 white prepupae 24hr    18 pupae 2d postWPP    26 pupae 3d postWPP    5 pupae 4d postWPP    33 adult male 01day    1 adult male 05day    1 adult male 30day    0 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    12 embryonic 1182-4H    5 embryonic GM2    5 embryonic Kc167    44 embryonic S1    719 embryonic S3    110 leg disc CME L1    26 wing disc CME-W2    20 wing disc ML-DmD8    1 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    26 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    83 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    74 ovary fGS/OSS    109 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    12 embryonic 1182-4H    5 embryonic GM2    5 embryonic Kc167  (44) embryonic S1  (719) embryonic S3  (110) leg disc CME L1  26 wing disc CME-W2    20 wing disc ML-DmD8    1 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    3 wing disc ML-DmD21  26 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1  (83) CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2  (33) tumorous blood cells mbn2  (74) ovary fGS/OSS  (109) ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    12 embryonic 1182-4H    5 embryonic GM2    5 embryonic Kc167    44 embryonic S1  (719) embryonic S3  (110) leg disc CME L1    26 wing disc CME-W2    20 wing disc ML-DmD8    1 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    26 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    83 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    74 ovary fGS/OSS  (109) ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    12 embryonic 1182-4H    5 embryonic GM2    5 embryonic Kc167    44 embryonic S1    719 embryonic S3    110 leg disc CME L1    26 wing disc CME-W2    20 wing disc ML-DmD8    1 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    26 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    83 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    74 ovary fGS/OSS    109 ovary OSC    3 ovary OSS    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    12 embryonic 1182-4H    5 embryonic GM2    5 embryonic Kc167    44 embryonic S1    719 embryonic S3    110 leg disc CME L1    26 wing disc CME-W2    20 wing disc ML-DmD8    1 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    26 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    83 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    74 ovary fGS/OSS    109 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    12 embryonic 1182-4H    5 embryonic GM2    5 embryonic Kc167  (44) embryonic S1  (719) embryonic S3  (110) leg disc CME L1  26 wing disc CME-W2    20 wing disc ML-DmD8    1 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    3 wing disc ML-DmD21  26 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1  (83) CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2  (33) tumorous blood cells mbn2  (74) ovary fGS/OSS  (109) ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    12 embryonic 1182-4H    5 embryonic GM2    5 embryonic Kc167    44 embryonic S1  (719) embryonic S3  110 leg disc CME L1    26 wing disc CME-W2    20 wing disc ML-DmD8    1 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    26 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    83 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    74 ovary fGS/OSS  109 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    12 embryonic 1182-4H    5 embryonic GM2    5 embryonic Kc167    44 embryonic S1    719 embryonic S3    110 leg disc CME L1    26 wing disc CME-W2    20 wing disc ML-DmD8    1 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    26 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    83 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    74 ovary fGS/OSS    109 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    10 heat shock, 4-day adult    277 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    122 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    296 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    10 heat shock, 4-day adult  (277) Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3  (27) Cadmium 50 mM 48 hrs, 4-day adult  (37) Cadmium 100 mM 48 hrs, 4-day adult  (56) Copper 0.5 mM 12 hrs, larvae L3  (122) Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3  (53) Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  (296) Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    10 heat shock, 4-day adult  (277) Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3  (122) Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  (296) Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    10 heat shock, 4-day adult    277 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    122 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    296 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    10 heat shock, 4-day adult    277 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    122 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    296 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    10 heat shock, 4-day adult  (277) Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3  27 Cadmium 50 mM 48 hrs, 4-day adult  (37) Cadmium 100 mM 48 hrs, 4-day adult  (56) Copper 0.5 mM 12 hrs, larvae L3  (122) Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3  (53) Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  (296) Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    10 heat shock, 4-day adult  (277) Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3  122 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  (296) Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    10 heat shock, 4-day adult    277 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    122 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    296 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_25
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013277 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6489-RA;CG31449-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Hsp70Ba304 Other Phenotypes Allele heat stress response defective Hsp70Bajockey Hsp70BaP.ArvZim Hsp70BaP.EC Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of Hsp70Ba Class Mutagen Stocks Known lesion Hsp70Ba304 FLPase SCEI endonuclease recombination ends-out gene targeting 2 Yes Hsp70Bajockey natural population 0 Yes Hsp70BaP.ArvZim natural population 0 Yes Hsp70BaP.EC P-element activity natural population 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hsp70Ba Class Mutagen Stocks Known lesion Hsp70BaGLV21037 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsp70BaHMS02661 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsp70BaNIG.31449R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)ED5579 (Takahashi et al., 2011) Df(3R)Exel7317 (Ryder, 2004.4.26) Df(3R)Hsp70B (Gong and Golic, 2004, Gong and Golic, 2005.6.2) Df(3R)kar1W (Gausz et al., 1981) Df(3R)kar3J (Gausz et al., 1981) Df(3R)kar3Q (Gausz et al., 1981) Df(3R)kar-D1 (Gausz et al., 1981) Df(3R)kar-D2 (Caggese et al., 1979) Df(3R)kar-H5 (Gausz et al., 1981) Df(3R)kar-H10 (Gausz et al., 1981) Df(3R)kar-Sz12 (Gausz et al., 1981) Df(3R)ry615 (Gausz et al., 1981)
Not disrupted in	Df(3R)E229 (Gausz et al., 1981) Df(3R)T-45 (Gausz et al., 1981) Df(3R)T-47 (Gausz et al., 1981) Df(3R)T-55 (Gausz et al., 1981)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.31449R} NA P{TRiP.GLV21037} NA P{TRiP.HMS02661} NA characterization construct P{tra+34} NA P{usptOa} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions jockey{3'}Hsp70Bajockey NA P{}Hsp70BaP.ArvZim NA P{}Hsp70BaP.EC NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
heat shock-mediated polytene chromosome puffing inferred from mutant phenotype (Gong and Golic, 2006) response to heat inferred from mutant phenotype (Gong and Golic, 2006) response to hypoxia inferred from expression pattern (Azad et al., 2009) inferred from mutant phenotype (Azad et al., 2009)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
response to heat non-traceable author statement (FlyBase, 1992-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Hsp70Ba - CG10984 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Ba - CG14262 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Ba - CG9596 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Ba - Csp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Ba - Gadd34 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Ba - Hsp27 experimental knowledge based (Guruharsha et al., 2011) Hsp70Ba - Hsp70Aa experimental knowledge based (Guruharsha et al., 2011) Hsp70Ba - Hsp70Bb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Ba - Pk17E anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Ba - mts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Ba - p53 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hsp70Ba allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69382 DroID - A comprehensive database of gene and protein interactions. • FBgn0013277 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44921
Orthologs
OrthoDB Orthologs (125) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG68KRWT Dipteran inclusive ortholog search EOG6SF9F4 Insect inclusive ortholog search EOG6B8H85 Arthropod inclusive ortholog search EOG6K98TB Metazoa inclusive ortholog search EOG609123
Orthologs in Drosophila Species (EOG68KRWT)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Hsp70Aa     Drosophila melanogaster fruit fly  Dmel\Hsp70Bbb     Drosophila melanogaster fruit fly  Dmel\Hsp70Ba     Drosophila melanogaster fruit fly  Dmel\Hsp70Ab     Drosophila melanogaster fruit fly  Dmel\Hsp70Bc     Drosophila melanogaster fruit fly  Dmel\Hsp70Bb     Drosophila simulans   Dsim\Hsp70Ba   Y Drosophila simulans   Dsim\Hsp70Ab   Y Drosophila simulans   Dsim\Hsp70Bb   Y Drosophila simulans   Dsim\Hsp70Aa   Y Drosophila sechellia   Dsec\GM23988   Y Drosophila sechellia   Dsec\GM26058   Y Drosophila erecta   Dere\GG19027   Y Drosophila erecta   Dere\GG10943   Y Drosophila erecta   Dere\GG17178   Y Drosophila erecta   Dere\GG19049   Y Drosophila erecta   Dere\GG18520   Y Drosophila erecta   Dere\GG17119   Y Drosophila erecta   Dere\GG19004   Y Drosophila ananassae   Dana\GF18384   Y Drosophila ananassae   Dana\GF16630   Y Drosophila ananassae   Dana\GF18385   Y Drosophila ananassae   Dana\GF16631   Y Drosophila ananassae   Dana\GF19283   Y Drosophila ananassae   Dana\GF16629   Y Drosophila ananassae   Dana\GF15894   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA26755   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA19632   Y Drosophila persimilis   Dper\GL27169   Y Drosophila willistoni   Dwil\GK10980   Y Drosophila willistoni   Dwil\GK13736   Y Drosophila willistoni   Dwil\GK13555   Y Drosophila virilis   Dvir\GJ10101   Y Drosophila virilis   Dvir\Hsp70g   Y Drosophila virilis   Dvir\Hsp70e   Y Drosophila virilis   Dvir\Hsp70f   Y Drosophila virilis   Dvir\Hsp70b   Y Drosophila virilis   Dvir\GJ10098   Y Drosophila virilis   Dvir\GJ14116   Y Drosophila virilis   Dvir\GJ10102   Y Drosophila virilis   Dvir\GJ18387   Y Drosophila virilis   Dvir\GJ16355   Y Drosophila virilis   Dvir\Hsp70a   Y Drosophila mojavensis   Dmoj\GI10253   Y Drosophila mojavensis   Dmoj\GI10256   Y Drosophila mojavensis   Dmoj\GI22499   Y Drosophila mojavensis   Dmoj\GI22498   Y Drosophila mojavensis   Dmoj\GI10255   Y Drosophila mojavensis   Dmoj\GI10254   Y Drosophila grimshawi   Dgri\GH17332   Y Drosophila grimshawi   Dgri\GH15224   Y Drosophila grimshawi   Dgri\GH23796   Y Drosophila grimshawi   Dgri\GH15213   Y
Orthologs in non-Drosophila Dipterans (EOG6SF9F4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801277 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801273 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801272 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801271 Y Aedes aegypti Yellow fever mosquito  Aaeg\Hsp70Cb Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801278 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ014283 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011082 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011083 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011081 Y
Orthologs in non-Dipteran Insects (EOG6B8H85)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002344 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002346 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003712 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008763 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI067751 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI007961 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI000903 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001597 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI009117 Y Bombyx mori Silkmoth  Bmor\BGIBMGA014536 Y Pediculus humanus Human body louse  Phum\PHUM126800 Y Tribolium castaneum Red flour beetle  Tcas\GLEAN_05094 Y Tribolium castaneum Red flour beetle  Tcas\hsp68b Y Tribolium castaneum Red flour beetle  Tcas\TC000188 Y Tribolium castaneum Red flour beetle  Tcas\hsp68b Y Tribolium castaneum Red flour beetle  Tcas\TC010172 Y
Orthologs in non-Insect Arthropods (EOG6K98TB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_256736 Y Daphnia pulex Water flea  Dpux\JGI_V11_302856 Y Daphnia pulex Water flea  Dpux\Rabgap1 Y Ixodes scapularis Deer tick  Isca\ISCW011425 Y Ixodes scapularis Deer tick  Isca\ISCW024910 Y
Orthologs in non-Arthropod Metazoa (EOG609123)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\F44E5.4 Y Caenorhabditis elegans Nematode  Cele\F44E5.5 Y Caenorhabditis elegans Nematode  Cele\hsp-70 Y Caenorhabditis elegans Nematode  Cele\hsp-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009477gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016500gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005808gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014864gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009164gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009165gn Y Danio rerio Zebrafish  Drer\hsp70 Y Danio rerio Zebrafish  Drer\zgc:63663 Y Danio rerio Zebrafish  Drer\hspa8l Y Danio rerio Zebrafish  Drer\si:ch211-199o1.2 Y Danio rerio Zebrafish  Drer\hspa8 Y Xenopus tropicalis Western clawed frog  Xtro\hspa1l Y Xenopus tropicalis Western clawed frog  Xtro\hspa1a Y Xenopus tropicalis Western clawed frog  Xtro\hspa8 Y Xenopus tropicalis Western clawed frog  Xtro\hspa1b Y Xenopus tropicalis Western clawed frog  Xtro\hsp70 Y Xenopus tropicalis Western clawed frog  Xtro\hspa2 Y Gallus gallus Domestic chicken  Ggal\HSP70 Y Gallus gallus Domestic chicken  Ggal\HSPA8 Y Mus musculus House mouse  Mmus\Hspa1a Y Mus musculus House mouse  Mmus\Hspa1l Y Mus musculus House mouse  Mmus\Hspa1b Y Mus musculus House mouse  Mmus\Hspa2 Y Mus musculus House mouse  Mmus\Hspa8 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000009920 Y Rattus norvegicus Norway rat  Rnor\Hspa8 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000034066 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022105 Y Rattus norvegicus Norway rat  Rnor\Hspa1a Y Rattus norvegicus Norway rat  Rnor\Hspa2 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029072 Y Homo sapiens Human  Hsap\HSPA8 Y Homo sapiens Human  Hsap\HSPA2 Y Homo sapiens Human  Hsap\ENSG00000204388 Y Homo sapiens Human  Hsap\HSPA1A Y Homo sapiens Human  Hsap\HSPA1L Y Homo sapiens Human  Hsap\HSPA6 Y
Human Orthologs (6)
Gene OMIM HGNC Hsap\HSPA8     Hsap\HSPA2     Hsap\ENSG00000204388     Hsap\HSPA1A     Hsap\HSPA1L     Hsap\HSPA6
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	8845 w1118; Hsp70Ba304 8844 w1118; Df(3R)Hsp70A, Hsp70Ba304 35672 y1 sc* v1; P{TRiP.GLV21037}attP2 43289 y1 sc* v1; P{TRiP.HMS02661}attP40
Genomic Clones ( 2 )
	BACR02G11 BACR28I14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 27 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT29796 LP03941 LP05203 LP05233 LP08776 LP11008 LP11561 LP13742 LP23554 RE48592
Other clones	DPiM_LP05203 DPiM_RE48592
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT25953 GH24523 GM12929 LP04534 LP15296 LP17176 LP20496 SD27902 UT01572
Other clones	214477.35 CK01.50B.H1 Dmel_neb_005321_0653_0721 EC054721 EK041629 RP003202220
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013277 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44921
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Flies with no copies of the Hsp70 genes (Hsp70Aa, Hsp70Ab, Hsp70Ba, Hsp70Bb, Hsp70Bbb and Hsp70Bc) are unable to survive a severe heat shock. These flies show a lengthened heat-shock response and developmental delay following a non-lethal heat shock. (Gong and Golic, 2006) Flies with no copies of the Hsp70 genes (Hsp70Aa, Hsp70Ab, Hsp70Ba, Hsp70Bb, Hsp70Bbb and Hsp70Bc) are viable and fertile. (Gong and Golic, 2004) Some Drosophila strains, including that sequenced in the Drosophila genome project have three, not two, tandemly repeated Hsp70 genes at 87C1 (in addition to a reverse orientation Hsp70 gene, Hsp70Ba, approximately 40kb upstream). The distal-most gene of the three tandem copies is Hsp70Bc. The inner two tandem copies are Hsp70Bb and Hsp70Bbb. (Bettencourt, 2002.1.16) New annotation (CG31449) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Area matching Drosophila heat shock gene at 87c,proximal end, Acc. No. K01294. (Davies, 2001.3.15) Natural variation in Hsp70 expression is analysed to study thermotolerance. (Krebs and Feder, 1997) The rate and intensity of Hsp70Ba protein expression has been compared with tissue damage after heat shock. (Krebs and Feder, 1997) Expression is rapidly induced in the testis after heat stress. Activation of Hsp70 in testes and heads necessitates the presence of a functional Hsf. (Michaud et al., 1997) There is significant variation among 74 different 2nd chromosome lines and 70 different 3rd chromosome lines in response to heat shock, measured by mRNA accumulation. (Otsuka et al., 1997) d(GA.TC)n sequences can be found in the promoters of Hsp26 and the Hsp70 genes. In vitro assembly of mononucleosomes into short DNA fragments carrying d(GA.TC)n sequences of different lengths is very efficient. Nucleosome assembly is inhibited strongly when the d(GA.TC)n sequence forms a triple-stranded conformation. Triplex formation requires partial destabilisation of the nucleosome. Results indicate nucleosome assembly and triplex formation are competing processes. (Espinas et al., 1996) Chromosome homologies of Muller's element D (J chromosome in the Paleartic species and XR chromosome arm in Nearctic species) and of element E (O chromosome in the Paleartic species and 2 chromosome in Nearctic species) have been confirmed by single copy probes in the species of the obscura group and in D.melanogaster. (Segarra et al., 1996) Sodium salicylate induces activation of Hsf binding activity in salivary gland cells and Schneider SL2 tissue cells. Puffing of heat shock gene loci occurs in salivary glands but Hsp70 transcription is not induced suggesting puffing and transcription are separable events. (Winegarden et al., 1996) The in vitro binding of Hsf protein to the promoter region of a number of heat shock genes has been analysed. (Fernandes et al., 1995) UV cross linking technique has been used to study the in vivo distribution of Trl protein on Hsp70 and Hsp26. Prior to heat shock Trl protein is associated with the promoter regions of the uninduced Hsp70 and Hsp26 genes. Upon heat shock induction Trl protein is recruited to their transcription units with its distribution coincident with that of RNA polymerase II. (O'Brien et al., 1995) The molecular architecture of the Hsp70Ba promoter has been analysed by genomic footprinting. (Weber and Gilmour, 1995) RNA levels do not increase with age, so the observed increase in protein levels is due to post-transcriptional regulation. Aging-specific expression may be a result of oxidative damage. (Wheeler et al., 1995) Synthesis of heat shock proteins is inhibited by both short-chain fatty acids and their corresponding alcohols, compounds which have no observable effect on histone acetylation. (Munks and Turner, 1994) Gene contains an RNA polymerase II complex which pauses after synthesis of a short transcript. In vivo ultraviolet crosslinking techniques demonstrate phosphorylation of the carboxy terminal domain (CTD) of the large subunit of RNA polymerase II could either regulate the transition of polymerase from a paused to an elongated complex or be a consequence of the transition from paused to elongated. (O'Brien et al., 1994) Chromosome staining reveals that Trl and heat shock transcription factors (HSF) colocalises at Hsp70Ba. (Tsukiyama et al., 1994) The 87A7 Hsp70B region is bordered on the proximal and distal sides by two special chromatin structures, scs and scs'. An enhancer blocking assay based on the w gene demonstrates that the two nuclease hypersensitive regions that define part of the scs chromatin structure are essential for the blocking activity. The DNA sequence spanning the nuclease resistant core located between the two hypersensitive regions is dispensable. (Vazquez and Schedl, 1994) The transcription of heat shock proteins, except Hsp83, is independent of the p200 subunit of initiation factor eIF-4F, eIF-4G. (Zapata et al., 1994) RNA polymerase distribution on uninduced Hsp70 genes has been compared with the distribution of RNA polymerase on DRB (5,6,-Dichloro-1-β-D-ribofuranosylbenzimidazole)-inhibited induced Hsp70 genes. (Giardina and Lis, 1993) Hsp70 proteins are of prime importance to heat tolerance. (Parsell et al., 1993) Members of the hsc70 gene family (heat shock cognate genes) that reside within the same intracellular compartment in different organisms share greater amino acid identity than hsc70 proteins from the same organism but different organelles. This pattern of conservation indicates specialisation of hsc70 function. (Rubin et al., 1993) Identified in 2D gels of CMW W2 wing imaginal disc cell proteins. (Santaren et al., 1993) Nascent chain nuclear run-on assays in KC161 cells reveal different responses to heat shock for different genes. Transcription of His1 is severely inhibited under mild heat shocks, of Act5C decreases proportionally with increasing temperature while that of the core histone genes or the heat shock cognates is repressed only under extreme heat shock. In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range. Hsrω is transcribed at a very high rate under non-heat shock conditions, and its response to elevated temperatures is different from that of the protein coding heat shock genes. (Vazquez et al., 1993) Hsp70 promoter driven expression in heat induced and non-heat induced conditions is more efficient in D.melanogaster embryos than in L.cuprina embryos. (Atkinson and O'Brochta, 1992) The interaction of Top1 and Top2 gene products with transcriptionally active and inactive Hsp70B has been compared. Topoisomerase I binding sites are found in the transcribed portions of the Hsp70B gene, and only when Hsp70B is transcriptionally active. Topoisomerase II binding to Hsp70B sequences occurs on both transcriptionally active and inactive chromatin. An unusual type of topoisomerase II binding site is associated with the 5' ends of inactive Hsp70 gene, suggesting that this enzyme may be associated with repression of gene transcription. (Kroeger and Rowe, 1992) The effect of aging on the expression of the "Hsp70" genes (Hsp70Aa, Hsp70Ab, Hsp70Ba, Hsp70Bb and Hsp70Bc) has been studied. (Niedzwiecki et al., 1991) Translation of Hsp70 mRNAs and to a lesser extent the mRNAs for the small heat shock proteins is almost independent of eIF-4E. (Zapata et al., 1991) Induction of heat shock protein after metal ion exposure is studied. (Bournias-Vardiabasis et al., 1990) Sequences located 5' to Sgs7, Sgs8, Sgs3, the Hsp70 genes at 87A and 87C and the copia coding region are similar to the sequence at -405 from Sgs4. (Shermoen and Beckendorf, 1982) initiates the heat-shock response. A restricted subset of genes, the Hsp genes, is activated and the majority of transcription and translation is shut down. However, mitochondrial- and histone-gene activities persist (Spradling, Pardue and Penman, 1977). This response follows a pulse of 36oC to 40oC; treatments above 40oC inhibit all activity and lead to death; treatments of 30oC-35oC induce heat-shock-protein synthesis without repressing normal protein synthesis (Tissieres, Mitchell and Tracy, 1974). Similar response inducible by other stressful treatments. The response may be elicited at all stages of the life cycle and in cultured cells. Stage specific phenocopies result from heat shocking early stages of Drosophila development (Mitchell and Petersen, 1982). In polytene cells existing puffs regress and a novel group quickly appears at 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D (Ashburner, 1970; Tissieres, Mitchell and Tracy, 1974). Activation of transcription of Hsp genes apparently involves the sequential binding of two or more protein factors in vicinity of TATA box (Wu, 1984). Binding sites for these proteins are multiple short upstream sequence elements called HSEs or heat shock consensus elements (Pelham, 1982; Xiao and Lis, 1988). Polymerase II dissociates from most chromosome regions and accumulates at the new puff sites (Bonner and Kerby, 1982). 3H-uridine incorporation ceases at its usual positions and commences at new puff sites. Preexisting polysomes disaggregate and within a few minutes a new population of polysomes appears containing newly transcribed mRNA; this RNA hybridizes to some of the heat-shock puffs. The effects of heat shock may be abrogated to some degree by pretreatment with a pulse of a slightly lower temperature (Mitchell, Moller, Petersen and Lipps-Sarmiento, 1979; Peterson and Mitchell, 1981). For reviews of the heat-shock response see Ashburner and Bonner (1978).   One of five structural genes (in two clusters, Hsp70A and Hsp70B) that code for the 70,000 dalton heat-shock protein (HSP70), the most abundant of the heat-shock proteins. Hsp70B usually includes three HSP70 encoding genes (Hsp70Ba (proximal), Hsp70Bb (middle), Hsp70Bc (distal)) (Holmgren, Livak, Morimoto, Freund, and Meselson, 1979) with slightly different restriction maps (Artavanis-Tsakonas et al., 1978). HSP70 returns to preshock levels more rapidly than other heat-shock proteins following return to 25oC (DiDomenico, Bugaisky and Lindquist, 1982). The protein becomes concentrated in nuclei during heat shock; disperses to cytoplasm during recovery; returns to nucleus upon further heat shock (Velazquez and Lindquist, 1984). Appears not to be expressed in the testis in response to heat-shock stimulation (Bonner, Parks, Parker-Thornberg, Mortin and Pelham, 1984). Deletion of either Hsp70A or Hsp70B does not eliminate the HSP70 heat-shock response; simultaneous deletion of both does (Ish-Horowicz et al., 1979).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_169469 RefSeq (Proteins) • NP_731716 DDBJ / EMBL / GenBank • A15908 AAG26900 AAG26901 AAG26902 AAG26903 AAG26904 AAK30225 AAK30226 AAK30227 AAK30228 AAK30229 AAK30230 AAK30231 AAK30232 AAK54833 AAK54836 AAK62472 AAK67154 AAK67155 AAK67156 AAK67157 AAN13545 AF295946 AF295947 AF295948 AF295949 AF295950 AF350468 AF350469 AF350470 AF350471 AF350472 AF350473 AF350474 AF350475 AF377341 AF377953 AF385405 AF385406 AF385407 AF385408 AX093917 AY032740 BI633747 CAA23500 K01293 K01294 M10178 V00219 Entrez Gene - A searchable database of RefSeq genes. • 44921 UniProtKB/Swiss-Prot • Q8INI8 UniProtKB/TrEMBL • Q95NM1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Heat shock protein 70, conserved site (IPR018181) Heat shock protein 70 family (IPR013126)
Linkouts
	BioGRID - A database of protein and genetic interactions • 69382 DroID - A comprehensive database of gene and protein interactions. • FBgn0013277 DRSC - Results from RNAi screens. • FBgn0013277 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013277 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6489-RA;CG31449-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013277 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44921 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44921 modMine - Data generated by the modENCODE project. • FBgn0013277
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	CG31449 (Hughes et al., 2008, Neal et al., 2006, Ni et al., 2011.7.19, Ni et al., 2010.12.1) dhsp70 (Rabindran et al., 1994) dHsp70 (Bell et al., 2007) Dm-hsp70 (Hernandez et al., 2004) Hsp70 (Baker and Russell, 2009, Auluck et al., 2005, Scholz et al., 2005, Ahmed and Duncan, 2004, Jennings et al., 2004, Zhai et al., 2004, Gruntenko et al., 2003, Morrow et al., 2004, Temme et al., 2004, Marsh and Thompson, 2004, Sathyanarayanan et al., 2004, Jin et al., 2003, Canamasas et al., 2003, Norry and Loeschcke, 2003, Rutherford, 2003, Silbermann and Tatar, 2000, Sejerkilde et al., 2003, Krebs, 1999, Lakhotia et al., 2002, Auluck and Bonini, 2003, Caletka and Fry, 2003, Shaw et al., 2002, Auluck et al., 2002, Rajendra et al., 2001, Sorensen and Loeschcke, 2001, Bulgheresi et al., 2001, Mok et al., 2001, Zatsepina et al., 2001, Kelty and Lee, 2001, de Carcer et al., 2001, Ekengren and Hultmark, 2001, Wu et al., 2001, Krebs et al., 2001, Andrulis et al., 2000, Gunawardena and Rykowski, 2000, White et al., 1999, Feder, 1999, Elefant and Palter, 1999, Jazwinski, 1998, Dahlgaard et al., 1998, Wang and Lindquist, 1998, Cossins, 1998, Gu, 1998, Ashburner et al., 1998, Krebs et al., 1998, Michaud et al., 1997, Krebs and Feder, 1997, Krebs and Feder, 1997, Krebs and Feder, 1997, Espinas et al., 1996, Winegarden et al., 1996, Kingston et al., 1996, Engel and Cornelius, 1995, Weber and Gilmour, 1995, Li and Duncan, 1995, O'Brien et al., 1995, Gehring and Wehner, 1995, Lis and Wu, 1994, Fernandes et al., 1994, Parsell et al., 1993, Evgen'ev and Denisenko, 1990, Papaconstantinou et al., 2006, Behr et al., 2006, Shilova et al., 2006, Fernandez-Funez et al., 2007, Sengupta and Lakhotia, 2006, Tenney et al., 2006, Smith et al., 2008, Ahrens et al., 2007, Zobeck et al., 2010, Hanyu-Nakamura et al., 2008, Eissenberg et al., 2007, Morettini et al., 2011, Bonini, 2002, Mito et al., 2007, Huen and Chan, 2005, Brandt and Corces, 2008, Newman et al., 2005, Yao et al., 2007, Ni et al., 2008, Posgai et al., 2009, Iijima-Ando et al., 2005, Cai et al., 2008, Gilchrist et al., 2008, Lerman and Feder, 1999, Chopra et al., 2009, Kalosaka et al., 2006, Nielsen et al., 2005, Overgaard et al., 2005, Kristensen et al., 2003, Liévens et al., 2008, Cobreros et al., 2008, Piacentini et al., 2009, Tian et al., 2010, Bartkowiak et al., 2010, Hrizo and Palladino, 2010, Kwon et al., 2010, Zobeck et al., 2010, Jensen et al., 2010, Krebs and Holbrook, 2001, Neal et al., 2006, Fujikake et al., 2008, Gupta et al., 2005, Duncan, 2005, McLear et al., 2008, Rezaval et al., 2007, Duncan, 2008, Bhargav et al., 2008, Schneiderman et al., 2012, Calabria et al., 2012, Fredriksson et al., 2012, Ardehali et al., 2009) hsp70 (Adelman et al., 2005, Wang et al., 2004, Matsumoto and Hirose, 2004, Landis et al., 2004, Smith et al., 2004, Ni et al., 2004, Saunders et al., 2005, Venkaiah et al., 2004, Lehmann, 2004, Viswanathan et al., 2003, Helfand and Rogina, 2003, Goodman et al., 2004, Tatar and Yin, 2001, Saunders et al., 2003, Kellum, 2003, Shaw and Franken, 2003, Feder, 2003, Geyer et al., 2000, Mahowald, 2001, Wimmer, 2003, Gilmour, 2003.4.18, Aigaki et al., 2002, Tulin and Spradling, 2003, Andrulis et al., 2002, Gong and Golic, 2003, Goodman et al., 2003, Kazantsev et al., 2002, Lakhotia and Prasanth, 2002, Herold et al., 2001, Gatfield et al., 2001, Biggin, 2001, Crevel et al., 2001, Christoffels et al., 2000, Badenhorst and Wu, 2001, Leach et al., 2000, Farkas et al., 2000, Yueh and Schneider, 2000, Lakhotia et al., 1999, Tatar, 1999, Feder, 1999, Hirayoshi and Lis, 1999, Bonini, 1999, Wu et al., 1998, Lis, 1998, Karunanithi et al., 1999, Mannervik, 1999, Sah et al., 1999, King and Tower, 1999, Katsani et al., 1999, Carr and Biggin, 1999, Zhao and Eggleston, 1999, Wilkins and Lis, 1999, Agianian et al., 1999, Nevo, 1998, Gdula et al., 1998, Yiangou et al., 1997, Wu and Fallon, 1997, Wilkins and Lis, 1998, Vernick and McCutchan, 1998, John and Workman, 1998, Curtsinger et al., 1997, Liang and Biggin, 1998, Gregory and Horz, 1998, Martinez-Balbas et al., 1998, Greenblatt, 1997, Wilkins and Lis, 1997, Finch and Tanzi, 1997, Otsuka et al., 1997, Tower, 1996, Shopland and Lis, 1996, Sandaltzopoulos et al., 1994, Segarra et al., 1996, O'Brien et al., 1994, Fernandes et al., 1995, Elefant and Palter, 1995, Wheeler et al., 1995, Rasmussen and Lis, 1995, Granok et al., 1995, Munks and Turner, 1994, Parsell and Lindquist, 1993, Zapata et al., 1994, Krumm et al., 1993, Fuller, 1993, Soeller et al., 1993, Lakhotia et al., 1993, Giardina and Lis, 1993, O'Brien and Lis, 1993, Molto et al., 1993, Heikkila, 1993, Papaceit and Juan, 1993, Vazquez et al., 1993, Morse, 1992, Fleming et al., 1992, Atkinson and O'Brochta, 1992, Pauli et al., 1992, Read and Manley, 1992, Becker et al., 1991, Niedzwiecki et al., 1991, Zapata et al., 1991, Bendena et al., 1991, Sorger, 1991, Spencer and Groudine, 1990, Helms and Rottman, 1990, Perkins et al., 1990, Karlin et al., 1990, Ornelles and Penman, 1990, Elgin, 1990, Menke and Petersen, 1989, Petersen and Lindquist, 1989, Petersen and Lindquist, 1988, Buszczak and Spradling, 2006, Kim and Lis, 2005, Graves and Tamkun, 2006, Halfon and Arnosti, 2007, Yao et al., 2006, Boeke et al., 2011, Wu et al., 2005, Piel et al., 2008, Schwartz et al., 2003, Boehm et al., 2003, Ullah et al., 2007, Ivaldi et al., 2007, Smith et al., 2008, Murawska et al., 2011, Behm-Ansmant et al., 2007, Kurshakova et al., 2007, Hsu et al., 2008, Bettencourt and Feder, 2000, Zhao et al., 2005, Li et al., 2001, Poels et al., 2004, Yang and Tower, 2009, Tombácz et al., 2009, Chan et al., 2011, Vazquez-Pianzola et al., 2007, Kopytova et al., 2010, Kotova et al., 2010, Juge et al., 2010, Weisbrot et al., 2003, Ardehali et al., 2011, Ghosh et al., 2011, Lebedeva et al., 2007, Lee et al., 2008, Grover et al., 2008, Siddique et al., 2008, Pankotai et al., 2010) HSP70 (Gerber et al., 2005, Aalfs and Kingston, 2000, Bondinas et al., 2002, Foley and Luckinbill, 2001, Bondinas et al., 2001, Driscoll and Gerstbrein, 2003, Zoghbi and Botas, 2002, Garbuz et al., 2002, Mitchell-Olds and Knight, 2002, Takada et al., 2000, Warrick et al., 1999, Mantovani, 1999, Takahashi and Fujiwara, 1999, Karlin and Brocchieri, 1998, Ogbourne and Antalis, 1998, Nover and Scharf, 1997, Jedlicka et al., 1997, Singh and Lakhotia, 1995, Rubin et al., 1993, Warrick and Boyd, 2006, Chartier et al., 2006, Papaconstantinou et al., 2005, Farny et al., 2008, Hurt et al., 2009, Folk et al., 2006, Sakaguchi et al., 2008) Hsp 70 (Zuckerkandl, 1997) hsp-70 (Luis et al., 1990, Belikov et al., 1990) hsp70 87C (Shermoen and Beckendorf, 1982) Hsp70(87C) (Henikoff, 1990) hsp70B (Gong and Golic, 2004, Huang et al., 2008) Hsp70B (Ranz et al., 2001, Whitfield, 2001.1.4, Ranz et al., 2001, Ashburner, 2000.10.30, Ruiz et al., 1997, Ranz et al., 1997, Buchon et al., 2009, Meisel, 2009, Chen et al., 2008) hsp70b (Yagi and Ip, 2005) hsp70Ba (Lerman et al., 2003, Bettencourt, 2002.1.16, Lerman et al., 2002, Bettencourt and Feder, 2001, Bettencourt et al., 1999, Bettencourt and Feder, 2002, Lerman and Feder, 2005) Hsp70Ba (Shilova et al., 2006, Baird et al., 2006, Kuan et al., 2009, Nikolaidis and Nei, 2004, Korol et al., 2006, Dorus et al., 2006, Moskalev et al., 2009, Xia and Holcik, 2009, Shieh and Bonini, 2011, Huang et al., 2008, Takahashi et al., 2011, Bettencourt et al., 2008, Azad et al., 2009, Haney and Feder, 2009, Bergman et al., 2006, Tian et al., 2010, Xi et al., 2008, Takahashi et al., 2010, Fisher et al., 2008, Cernilogar et al., 2011, Japanese National Institute of Genetics, 2012.5.21) hsp70 Ba (Kellett and McKechnie, 2005)
Name Synonym	87C locus (Lakhotia et al., 1990) heat shock 70 (Lo et al., 2002) heat-shock protein 70 (Iijima-Ando et al., 2005, Farny et al., 2008) Heat-shock-protein-70Ba   hsp70 87C (Casper et al., 2011) Protein 70 (Bournias-Vardiabasis et al., 1990)
Secondary FlyBase IDs
	FBgn0001232 FBgn0051449
References ( 427 )
Generate a list of	All references relating to this gene ( 427 )
List References by type	Research paper  ( 311 ) Supplementary material  ( 7 ) Review  ( 57 ) Retraction  ( 1 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 25 ) FlyBase analysis  ( 2 ) Book review  ( 1 ) Letter  ( 1 ) Conference report  ( 2 ) Stock list  ( 1 ) Patent  ( 1 ) DNA/RNA sequence record  ( 4 ) Book  ( 1 )
Recent research papers ( 15 )
	Sørensen et al., 2013, J. Exp. Biol. 216(5): 809--814 Cellular damage as induced by high temperature is dependent on rate of temperature change - investigating consequences of ramping rates on molecular and organismal phenotypes in Drosophila melanogaster. [FBrf0220807] Calabria et al., 2012, J. Evol. Biol. 25(4): 691--700 Hsp70 protein levels and thermotolerance in Drosophila subobscura: a reassessment of the thermal co-adaptation hypothesis. [FBrf0217786] Coléno-Costes et al., 2012, PLoS Genet. 8(10): e1003006 New partners in regulation of gene expression: the enhancer of trithorax and polycomb corto interacts with methylated ribosomal protein l12 via its chromodomain. [FBrf0219702] Fredriksson et al., 2012, Aging Cell 11(4): 634--643 Effects of aging and reproduction on protein quality control in soma and gametes of Drosophila melanogaster. [FBrf0218923] Schneiderman et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(48): 19721--19726 Nucleosome-depleted chromatin gaps recruit assembly factors for the H3.3 histone variant. [FBrf0220071] Ardehali et al., 2011, EMBO J. 30(14): 2817--2828 Drosophila Set1 is the major histone H3 lysine 4 trimethyltransferase with role in transcription. [FBrf0214333] Boeke et al., 2011, PLoS ONE 6(6): e20761 The RNA Helicase Rm62 Cooperates with SU(VAR)3-9 to Re-Silence Active Transcription in Drosophila melanogaster. [FBrf0213941] Casper et al., 2011, Development 138(16): 3357--3366 no child left behind encodes a novel chromatin factor required for germline stem cell maintenance in males but not females. [FBrf0214565] Cernilogar et al., 2011, Nature 480(7377): 391--395 Chromatin-associated RNA interference components contribute to transcriptional regulation in Drosophila. [FBrf0216979] Chan et al., 2011, Hum. Mol. Genet. 20(9): 1738--1750 Expanded polyglutamine domain possesses nuclear export activity which modulates subcellular localization and toxicity of polyQ disease protein via exportin-1. [FBrf0214367] Ghosh et al., 2011, Mol. Cell. Biol. 31(20): 4232--4243 Negative Elongation Factor Accelerates the Rate at Which Heat Shock Genes Are Shut off by Facilitating Dissociation of Heat Shock Factor. [FBrf0215827] Morettini et al., 2011, Nucleic Acids Res. 39(8): 3103--3115 The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization. [FBrf0213515] Murawska et al., 2011, PLoS Genet. 7(7): e1002206 Stress-Induced PARP Activation Mediates Recruitment of Drosophila Mi-2 to Promote Heat Shock Gene Expression. [FBrf0214629] Shieh and Bonini, 2011, Hum. Mol. Genet. 20(24): 4810--4821 Genes and pathways affected by CAG-repeat RNA-based toxicity in Drosophila. [FBrf0216738] Takahashi et al., 2011, PLoS ONE 6(4): e17295 Environmental Stress-Dependent Effects of Deletions Encompassing Hsp70Ba on Canalization and Quantitative Trait Asymmetry in Drosophila melanogaster. [FBrf0213649] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...