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Gene Dmel\Hsp70Bb

General Information
Symbol	Dmel\Hsp70Bb	Species	D. melanogaster
Name	Heat-shock-protein-70Bb	Annotation symbol	CG31359
Feature type	protein_coding_gene	FlyBase ID	FBgn0013278
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	Hsp70, hsp70B, Hsp70Bc, dhsp70, hsp-70
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	87B14-87B14	Sequence location	3R:8,331,515..8,333,861 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heat-shock-protein-70Bb is referred to in FlyBase by the symbol Dmel\Hsp70Bb (CG31359, FBgn0013278). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: response to hypoxia; heat shock-mediated polytene chromosome puffing; response to heat. 46 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Heat shock protein 70 family; Heat shock protein 70, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: No FlyAtlas data available because no Affy2 ProbeSet aligns to an exon of Hsp70Bb. Comments on Affy2 ProbeSet: No relevant information because no Affy2 ProbeSet aligns to an exon of Hsp70Bb. Gene sequence location is 3R:8331515..8333861.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Hsp70 The structural genes that code for the 70,000 dalton heat-shock protein (HSP70), the most abundant of the heat-shock proteins. HSP70 returns to preshock levels more rapidly than other heat-shock proteins following return to 25 (DiDomenico, Bugaisky, and Lindquist, 1982, Proc. Nat. Acad. Sci. USA 79: 6181-85). The protein becomes concentrated in nuclei during heat shock; disperses to cytoplasm during recovery; returns to nucleus upon further heat shock (Velazquez and Lindquist, 1984, Cell 36: 655-62). Appears not to be expressed in the testis in response to heat-shock stimulation (Bonner, Parks, Parker-Thornberg, Mortin, and Pelham, 1984, Cell 37: 979-91). Deletion of either the 87A7 or the 87C1 sequences does not eliminate the HSP70 heat-shock response; simultaneous deletion of both sequences does eliminate the HSP70 heat-shock response (Ish-Horowitz et al., 1979).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87B14-87B14   Limits computationally determined from genome sequence between P{PZ}svp07842 and P{lacW}Vha55j2E9
Experimentally Determined Cytological Location
Cytogenetic map Notes References 87C1-87C1   (determined by in situ hybridisation)   (Ranz et al., 1997) 87C-87C   (determined by in situ hybridisation)   (Segarra et al., 1996) 87C-87C   (determined by in situ hybridisation)   (Papaceit and Juan, 1993) 87C1-87C1   (determined by in situ hybridisation)   (Lis et al., 1981) 87C1-87C1   (determined by in situ hybridisation)   (Schedl et al., 1978)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hsp70Bb for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hsp70Bb-RA FBtr0082637  NM_080188   2347   641
Additional Transcript Data & Comments
Reported size (kB)	2.55 (northern blot) (Holmgren et al., 1979)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hsp70Bb-PA   FBpp0082106   70.2   641   5.49     NP_524927   AAN13546
Additional Polypeptide Data & Comments
Reported size (kDa)	70 (kD) (Holmgren et al., 1979)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Heat shock protein 70, conserved site (IPR018181) Heat shock protein 70 family (IPR013126)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392135   AA735988   AA801760   AA802144   AE014297 AAN13546   AF295951 AAG26905   AF295952 AAG26906   AF295953 AAG26907   AF295954 AAG26908   AF295955 AAG26909   AF295956 AAG26910   AF295957 AAG26911   AF350476 AAK30233   AF350477 AAK30234   AF350478 AAK30235   AF350479 AAK30236   AF350480 AAK30237   AF350481 AAK30238   AF350482 AAK30239   AF350483 AAK30240   AI257020   AI259195   AI260606   AI296097   AI404714   AI519655   AK256004   AY094878   AY118457   AY370940 AAQ75092   BF489797   BF489994   BF502721   BG640842   BI230151   BI351698   BI353624   BI353861   BI354093   BI637649   BI641201   BT011058   BT011541   BT021339   BT031349   BT099626   BT126332   BT126347   BX029450   CK604959   CK656982   CO188710   CO189409   CO264867   CO299843   CO309106   CO322369   CO327137   CO328458   CO332964   CS162814   DQ093386   DQ093387   EC054721   EC066340   EC080640   EC090689   EC222693   EC241193   EC251634   EC253197   EL885120   EV598402   J01104 AAD15226   K01861   M10229
UniProtKB/Swiss-Prot	Q9BIS2
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    182 central nervous system, larvae L3    3 central nervous system, pupae P8    24 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    18 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    3 head, mated 1-day male    10 head, mated 4-day male    3 head, mated 20-day male    7 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    28 digestive system, 4-day adult    60 digestive system, 20-day adult    28 fat body, larvae L3 wandering    51 fat body, white prepupae    49 fat body, pupae P8    46 carcass, larvae L3 wandering    14 carcass, 1-day adult    95 carcass, 4-day adult    59 carcass, 20-day adult    35 ovary, virgin 4-day female    0 ovary, mated 4-day female    2 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (182) central nervous system, larvae L3    3 central nervous system, pupae P8    24 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    18 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    3 head, mated 1-day male    10 head, mated 4-day male    3 head, mated 20-day male    7 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    11 digestive system, 1-day adult  (28) digestive system, 4-day adult  (60) digestive system, 20-day adult  (28) fat body, larvae L3 wandering  (51) fat body, white prepupae  (49) fat body, pupae P8  (46) carcass, larvae L3 wandering    14 carcass, 1-day adult  (95) carcass, 4-day adult  (59) carcass, 20-day adult  (35) ovary, virgin 4-day female    0 ovary, mated 4-day female    2 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (182) central nervous system, larvae L3    3 central nervous system, pupae P8    24 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    18 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    3 head, mated 1-day male    10 head, mated 4-day male    3 head, mated 20-day male    7 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    28 digestive system, 4-day adult    60 digestive system, 20-day adult    28 fat body, larvae L3 wandering    51 fat body, white prepupae    49 fat body, pupae P8    46 carcass, larvae L3 wandering    14 carcass, 1-day adult    95 carcass, 4-day adult    59 carcass, 20-day adult    35 ovary, virgin 4-day female    0 ovary, mated 4-day female    2 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    182 central nervous system, larvae L3    3 central nervous system, pupae P8    24 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    18 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    3 head, mated 1-day male    10 head, mated 4-day male    3 head, mated 20-day male    7 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    28 digestive system, 4-day adult    60 digestive system, 20-day adult    28 fat body, larvae L3 wandering    51 fat body, white prepupae    49 fat body, pupae P8    46 carcass, larvae L3 wandering    14 carcass, 1-day adult    95 carcass, 4-day adult    59 carcass, 20-day adult    35 ovary, virgin 4-day female    0 ovary, mated 4-day female    2 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    182 central nervous system, larvae L3    3 central nervous system, pupae P8    24 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    18 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    3 head, mated 1-day male    10 head, mated 4-day male    3 head, mated 20-day male    7 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    28 digestive system, 4-day adult    60 digestive system, 20-day adult    28 fat body, larvae L3 wandering    51 fat body, white prepupae    49 fat body, pupae P8    46 carcass, larvae L3 wandering    14 carcass, 1-day adult    95 carcass, 4-day adult    59 carcass, 20-day adult    35 ovary, virgin 4-day female    0 ovary, mated 4-day female    2 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (182) central nervous system, larvae L3    3 central nervous system, pupae P8    24 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    18 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    3 head, mated 1-day male    10 head, mated 4-day male    3 head, mated 20-day male    7 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    11 digestive system, 1-day adult  28 digestive system, 4-day adult  (60) digestive system, 20-day adult  28 fat body, larvae L3 wandering  (51) fat body, white prepupae  (49) fat body, pupae P8  (46) carcass, larvae L3 wandering    14 carcass, 1-day adult  (95) carcass, 4-day adult  (59) carcass, 20-day adult  (35) ovary, virgin 4-day female    0 ovary, mated 4-day female    2 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (182) central nervous system, larvae L3    3 central nervous system, pupae P8    24 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    18 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    3 head, mated 1-day male    10 head, mated 4-day male    3 head, mated 20-day male    7 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    28 digestive system, 4-day adult    60 digestive system, 20-day adult    28 fat body, larvae L3 wandering    51 fat body, white prepupae    49 fat body, pupae P8    46 carcass, larvae L3 wandering    14 carcass, 1-day adult    95 carcass, 4-day adult    59 carcass, 20-day adult    35 ovary, virgin 4-day female    0 ovary, mated 4-day female    2 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    182 central nervous system, larvae L3    3 central nervous system, pupae P8    24 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    18 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    3 head, mated 1-day male    10 head, mated 4-day male    3 head, mated 20-day male    7 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    28 digestive system, 4-day adult    60 digestive system, 20-day adult    28 fat body, larvae L3 wandering    51 fat body, white prepupae    49 fat body, pupae P8    46 carcass, larvae L3 wandering    14 carcass, 1-day adult    95 carcass, 4-day adult    59 carcass, 20-day adult    35 ovary, virgin 4-day female    0 ovary, mated 4-day female    2 testis, mated 4-day male    10 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    11 embryo 10-12hr    36 embryo 12-14hr    8 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    3 embryo 20-22hr    9 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    71 white prepupae 24hr    35 pupae 2d postWPP    49 pupae 3d postWPP    8 pupae 4d postWPP    75 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    11 embryo 10-12hr  (36) embryo 12-14hr    8 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    3 embryo 20-22hr    9 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr  (71) white prepupae 24hr  (35) pupae 2d postWPP  (49) pupae 3d postWPP    8 pupae 4d postWPP  (75) adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    11 embryo 10-12hr    36 embryo 12-14hr    8 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    3 embryo 20-22hr    9 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    71 white prepupae 24hr    35 pupae 2d postWPP    49 pupae 3d postWPP    8 pupae 4d postWPP    75 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    11 embryo 10-12hr    36 embryo 12-14hr    8 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    3 embryo 20-22hr    9 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    71 white prepupae 24hr    35 pupae 2d postWPP    49 pupae 3d postWPP    8 pupae 4d postWPP    75 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    2 adult female 30day    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    11 embryo 10-12hr    36 embryo 12-14hr    8 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    3 embryo 20-22hr    9 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    71 white prepupae 24hr    35 pupae 2d postWPP    49 pupae 3d postWPP    8 pupae 4d postWPP    75 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    11 embryo 10-12hr  (36) embryo 12-14hr    8 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    3 embryo 20-22hr    9 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr  (71) white prepupae 24hr  (35) pupae 2d postWPP  (49) pupae 3d postWPP    8 pupae 4d postWPP  (75) adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    11 embryo 10-12hr    36 embryo 12-14hr    8 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    3 embryo 20-22hr    9 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    71 white prepupae 24hr    35 pupae 2d postWPP    49 pupae 3d postWPP    8 pupae 4d postWPP    75 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    11 embryo 10-12hr    36 embryo 12-14hr    8 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    3 embryo 20-22hr    9 embryo 22-24hr    4 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    71 white prepupae 24hr    35 pupae 2d postWPP    49 pupae 3d postWPP    8 pupae 4d postWPP    75 adult male 01day    2 adult male 05day    2 adult male 30day    1 adult female 01day    1 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    50 embryonic 1182-4H    15 embryonic GM2    18 embryonic Kc167    203 embryonic S1    2064 embryonic S3    405 leg disc CME L1    98 wing disc CME-W2    120 wing disc ML-DmD8    5 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    76 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    292 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    157 tumorous blood cells mbn2    337 ovary fGS/OSS    270 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4  (50) embryonic 1182-4H    15 embryonic GM2    18 embryonic Kc167  (203) embryonic S1  (2064) embryonic S3  (405) leg disc CME L1  (98) wing disc CME-W2  (120) wing disc ML-DmD8    5 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21  (76) wing disc ML-DmD32    6 haltere disc ML-DmD17-c3  (40) eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1  (292) CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2  (157) tumorous blood cells mbn2  (337) ovary fGS/OSS  (270) ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    50 embryonic 1182-4H    15 embryonic GM2    18 embryonic Kc167  (203) embryonic S1  (2064) embryonic S3  (405) leg disc CME L1    98 wing disc CME-W2  (120) wing disc ML-DmD8    5 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    76 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1  (292) CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2  (157) tumorous blood cells mbn2  (337) ovary fGS/OSS  (270) ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    50 embryonic 1182-4H    15 embryonic GM2    18 embryonic Kc167    203 embryonic S1    2064 embryonic S3    405 leg disc CME L1    98 wing disc CME-W2    120 wing disc ML-DmD8    5 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    76 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    292 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    157 tumorous blood cells mbn2    337 ovary fGS/OSS    270 ovary OSC    9 ovary OSS    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    50 embryonic 1182-4H    15 embryonic GM2    18 embryonic Kc167    203 embryonic S1    2064 embryonic S3    405 leg disc CME L1    98 wing disc CME-W2    120 wing disc ML-DmD8    5 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    76 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    292 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    157 tumorous blood cells mbn2    337 ovary fGS/OSS    270 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4  (50) embryonic 1182-4H    15 embryonic GM2    18 embryonic Kc167  (203) embryonic S1  (2064) embryonic S3  (405) leg disc CME L1  (98) wing disc CME-W2  (120) wing disc ML-DmD8    5 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21  (76) wing disc ML-DmD32    6 haltere disc ML-DmD17-c3  (40) eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1  (292) CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2  (157) tumorous blood cells mbn2  (337) ovary fGS/OSS  (270) ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    50 embryonic 1182-4H    15 embryonic GM2    18 embryonic Kc167  (203) embryonic S1  (2064) embryonic S3  (405) leg disc CME L1    98 wing disc CME-W2  120 wing disc ML-DmD8    5 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    76 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1  (292) CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2  (157) tumorous blood cells mbn2  (337) ovary fGS/OSS  (270) ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    50 embryonic 1182-4H    15 embryonic GM2    18 embryonic Kc167    203 embryonic S1    2064 embryonic S3    405 leg disc CME L1    98 wing disc CME-W2    120 wing disc ML-DmD8    5 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    76 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    292 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    157 tumorous blood cells mbn2    337 ovary fGS/OSS    270 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    26 heat shock, 4-day adult    847 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    83 Cadmium 50 mM 48 hrs, 4-day adult    116 Cadmium 100 mM 48 hrs, 4-day adult    180 Copper 0.5 mM 12 hrs, larvae L3    250 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    139 Zinc 4.5 mM 48 hrs, 4-day adult    34 Ethanol 2.5% 3 hrs, larvae L3    757 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    29 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    21 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    65 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (41) cold shock, 4-day adult  26 heat shock, 4-day adult  (847) Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3  (83) Cadmium 50 mM 48 hrs, 4-day adult  (116) Cadmium 100 mM 48 hrs, 4-day adult  (180) Copper 0.5 mM 12 hrs, larvae L3  (250) Copper 15 mM 48 hrs, 4-day adult  (27) Zinc 5 mM 12 hrs, larvae L3  (139) Zinc 4.5 mM 48 hrs, 4-day adult  (34) Ethanol 2.5% 3 hrs, larvae L3  (757) Ethanol 5% 3 hrs, larvae L3  (36) Ethanol 10% 3 hrs, larvae L3  (29) Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    21 Caffeine 25 mg/ml 48 hrs, 4-day adult  (34) Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult  (65) Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3  (32) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    26 heat shock, 4-day adult  (847) Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    83 Cadmium 50 mM 48 hrs, 4-day adult  (116) Cadmium 100 mM 48 hrs, 4-day adult  (180) Copper 0.5 mM 12 hrs, larvae L3  (250) Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3  (139) Zinc 4.5 mM 48 hrs, 4-day adult    34 Ethanol 2.5% 3 hrs, larvae L3  (757) Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    29 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    21 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    65 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    26 heat shock, 4-day adult    847 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    83 Cadmium 50 mM 48 hrs, 4-day adult    116 Cadmium 100 mM 48 hrs, 4-day adult    180 Copper 0.5 mM 12 hrs, larvae L3    250 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    139 Zinc 4.5 mM 48 hrs, 4-day adult    34 Ethanol 2.5% 3 hrs, larvae L3    757 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    29 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    21 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    65 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    32 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    26 heat shock, 4-day adult    847 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    83 Cadmium 50 mM 48 hrs, 4-day adult    116 Cadmium 100 mM 48 hrs, 4-day adult    180 Copper 0.5 mM 12 hrs, larvae L3    250 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    139 Zinc 4.5 mM 48 hrs, 4-day adult    34 Ethanol 2.5% 3 hrs, larvae L3    757 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    29 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    21 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    65 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (41) cold shock, 4-day adult  26 heat shock, 4-day adult  (847) Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3  (83) Cadmium 50 mM 48 hrs, 4-day adult  (116) Cadmium 100 mM 48 hrs, 4-day adult  (180) Copper 0.5 mM 12 hrs, larvae L3  (250) Copper 15 mM 48 hrs, 4-day adult  27 Zinc 5 mM 12 hrs, larvae L3  (139) Zinc 4.5 mM 48 hrs, 4-day adult  (34) Ethanol 2.5% 3 hrs, larvae L3  (757) Ethanol 5% 3 hrs, larvae L3  (36) Ethanol 10% 3 hrs, larvae L3  29 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    21 Caffeine 25 mg/ml 48 hrs, 4-day adult  (34) Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult  (65) Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3  32 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    26 heat shock, 4-day adult  (847) Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    83 Cadmium 50 mM 48 hrs, 4-day adult  116 Cadmium 100 mM 48 hrs, 4-day adult  (180) Copper 0.5 mM 12 hrs, larvae L3  (250) Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3  139 Zinc 4.5 mM 48 hrs, 4-day adult    34 Ethanol 2.5% 3 hrs, larvae L3  (757) Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    29 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    21 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    65 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    26 heat shock, 4-day adult    847 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    83 Cadmium 50 mM 48 hrs, 4-day adult    116 Cadmium 100 mM 48 hrs, 4-day adult    180 Copper 0.5 mM 12 hrs, larvae L3    250 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    139 Zinc 4.5 mM 48 hrs, 4-day adult    34 Ethanol 2.5% 3 hrs, larvae L3    757 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    29 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    21 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    65 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_25 mE1_20_mRNA_expression_cluster_08
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013278 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6489-RA;CG31449-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Other Phenotypes Allele aging defective | adult stage | male limited | heat sensitive Hsp70Bb+t14 Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Hsp70Bb Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 46 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hsp70Bb Class Mutagen Stocks Known lesion Hsp70BbHMS00797 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsp70BbHMS00900 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsp70Bb+t14 in vitro construct - genomic fragment 0 Yes Hsp70Bb-194:+204 in vitro construct 0 Yes Hsp70Bb-194:+411 in vitro construct 0 Yes Hsp70Bb-194:+65 in vitro construct 0 Yes Hsp70Bb-194:-51 in vitro construct 0 Yes Hsp70Bb301.hs.MtnA in vitro construct - regulatory fusion 0 Yes Hsp70Bb301.MtnA in vitro construct - regulatory fusion 0 Yes Hsp70Bb301 in vitro construct 0 Yes Hsp70Bb306 in vitro construct 0 Yes Hsp70Bb309 in vitro construct - deletion 0 Yes Hsp70Bb420 in vitro construct - deletion 0 Yes Hsp70Bb45fs in vitro construct 0 Yes Hsp70Bb70ΔB in vitro construct - deletion 0 Yes Hsp70BbEip71CD.300 in vitro construct - regulatory fusion 0 Yes Hsp70Bbfl.hs.T:Rat\subP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Hsp70BbH0.MtnA in vitro construct - deletion 0 Yes Hsp70BbH1 in vitro construct - site directed mutagenesis 0 Yes Hsp70BbH2 in vitro construct - site directed mutagenesis 0 Yes Hsp70BbH3 in vitro construct - site directed mutagenesis 0 Yes Hsp70BbH4 in vitro construct - site directed mutagenesis 0 Yes Hsp70BbH5 in vitro construct 0 Yes Hsp70BbH6 in vitro construct 0 Yes Hsp70BbH7.MtnA in vitro construct - deletion 0 Yes Hsp70BbH8.MtnA in vitro construct - deletion 0 Yes Hsp70BbHsp83.FL.MtnA in vitro construct - regulatory fusion 0 Yes Hsp70BbHsp83.Δ110-AUG.MtnA in vitro construct - deletion 0 Yes Hsp70BbHsp83.Δ110-CAP.MtnA in vitro construct - deletion 0 Yes Hsp70BbHsp83.Δ40-110-I.MtnA in vitro construct - deletion 0 Yes Hsp70BbHsp83.Δ40-AUG.MtnA in vitro construct - deletion 0 Yes Hsp70BbHsp83.Δ40-CAP.MtnA in vitro construct - deletion 0 Yes Hsp70BbHsp83.Δ75-AUG.MtnA in vitro construct - deletion 0 Yes Hsp70BbHsp83.Δ75-CAP.MtnA in vitro construct - deletion 0 Yes Hsp70BbMtnA.PF in vitro construct - regulatory fusion 0 Yes Hsp70BbNIG.31359R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Hsp70BbpolyA in vitro construct 0 Yes Hsp70BbSgs3.PF in vitro construct - regulatory fusion 0 Yes Hsp70BbSL17.11.MtnA in vitro construct 0 Yes Hsp70BbSL17.6.MtnA in vitro construct 0 Yes Hsp70BbSL17.c.MtnA in vitro construct 0 Yes Hsp70BbSL60.11.MtnA in vitro construct 0 Yes Hsp70BbSL60.16.MtnA in vitro construct 0 Yes Hsp70BbSL60.22.MtnA in vitro construct 0 Yes Hsp70BbWTfs in vitro construct 0 Yes Hsp70BbΔ.hs.T:Rat\subP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel7317 (Ryder, 2004.4.26) Df(3R)Hsp70B (Gong and Golic, 2004, Gong and Golic, 2005.6.2) Df(3R)kar-D2 (Caggese et al., 1979)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{70Z(I4x2)} No P{70Z(I8)} No P{cBs+127Z} No P{cBs+331Z} No P{cp70ZT} No P{dHS1Ax4} No P{dHS1Bx4} No P{dHS1x4} No P{Hsp70Bb-lacZ.-194.+276} No P{hsp70-lacZ} No P{HZ.w} No P{pHS1Ax4} No P{pHS1Bx4} No P{pHS1x4.NB} No P{pHS1x4} No P{TTAGAhsp70-lacZ} No UAS construct P{GD1699} NA P{GD17637} NA P{NIG.31359R} NA P{TRiP.HMS00797} NA P{TRiP.HMS00900} NA GAL4 construct P{Hsp70.tTA.Ba} No characterization construct P{cp70ΔB} NA P{FRT(whs,3Xwt70).A} NA P{HBΔ-23} NA P{HBΔ-59} NA P{HBΔ-73} NA P{HBΔ-89} NA P{HBΔ-194} NA P{Hsp70B-51} NA P{Hsp70B+65} NA P{Hsp70B+204} NA P{Hsp70B+411} NA P{sgs70} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
heat shock-mediated polytene chromosome puffing inferred from mutant phenotype (Gong and Golic, 2006) response to heat inferred from mutant phenotype (Gong and Golic, 2006) response to hypoxia inferred from expression pattern (Azad et al., 2009) inferred from mutant phenotype (Azad et al., 2009)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
response to heat non-traceable author statement (FlyBase, 1992-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Hsp70Bb - Bap55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - CG5746 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Cp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - DnaJ-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Hsc70Cb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Hsp68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Hsp70Aa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Hsp70Ba anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Psi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Upf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Vha55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Wsck anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - qkr54B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp70Bb - tub anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hsp70Bb allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 71512 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0013278 DroID - A comprehensive database of gene and protein interactions. • FBgn0013278 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 48582
Orthologs
OrthoDB Orthologs (125) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG68KRWT Dipteran inclusive ortholog search EOG6SF9F4 Insect inclusive ortholog search EOG6B8H85 Arthropod inclusive ortholog search EOG6K98TB Metazoa inclusive ortholog search EOG609123
Orthologs in Drosophila Species (EOG68KRWT)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Hsp70Aa     Drosophila melanogaster fruit fly  Dmel\Hsp70Bbb     Drosophila melanogaster fruit fly  Dmel\Hsp70Ba     Drosophila melanogaster fruit fly  Dmel\Hsp70Ab     Drosophila melanogaster fruit fly  Dmel\Hsp70Bc     Drosophila melanogaster fruit fly  Dmel\Hsp70Bb     Drosophila simulans   Dsim\Hsp70Ba   Y Drosophila simulans   Dsim\Hsp70Ab   Y Drosophila simulans   Dsim\Hsp70Bb   Y Drosophila simulans   Dsim\Hsp70Aa   Y Drosophila sechellia   Dsec\GM23988   Y Drosophila sechellia   Dsec\GM26058   Y Drosophila erecta   Dere\GG19027   Y Drosophila erecta   Dere\GG10943   Y Drosophila erecta   Dere\GG17178   Y Drosophila erecta   Dere\GG19049   Y Drosophila erecta   Dere\GG18520   Y Drosophila erecta   Dere\GG17119   Y Drosophila erecta   Dere\GG19004   Y Drosophila ananassae   Dana\GF18384   Y Drosophila ananassae   Dana\GF16630   Y Drosophila ananassae   Dana\GF18385   Y Drosophila ananassae   Dana\GF16631   Y Drosophila ananassae   Dana\GF19283   Y Drosophila ananassae   Dana\GF16629   Y Drosophila ananassae   Dana\GF15894   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA26755   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA19632   Y Drosophila persimilis   Dper\GL27169   Y Drosophila willistoni   Dwil\GK10980   Y Drosophila willistoni   Dwil\GK13736   Y Drosophila willistoni   Dwil\GK13555   Y Drosophila virilis   Dvir\GJ10101   Y Drosophila virilis   Dvir\Hsp70g   Y Drosophila virilis   Dvir\Hsp70e   Y Drosophila virilis   Dvir\Hsp70f   Y Drosophila virilis   Dvir\Hsp70b   Y Drosophila virilis   Dvir\GJ10098   Y Drosophila virilis   Dvir\GJ14116   Y Drosophila virilis   Dvir\GJ10102   Y Drosophila virilis   Dvir\GJ18387   Y Drosophila virilis   Dvir\GJ16355   Y Drosophila virilis   Dvir\Hsp70a   Y Drosophila mojavensis   Dmoj\GI10253   Y Drosophila mojavensis   Dmoj\GI10256   Y Drosophila mojavensis   Dmoj\GI22499   Y Drosophila mojavensis   Dmoj\GI22498   Y Drosophila mojavensis   Dmoj\GI10255   Y Drosophila mojavensis   Dmoj\GI10254   Y Drosophila grimshawi   Dgri\GH17332   Y Drosophila grimshawi   Dgri\GH15224   Y Drosophila grimshawi   Dgri\GH23796   Y Drosophila grimshawi   Dgri\GH15213   Y
Orthologs in non-Drosophila Dipterans (EOG6SF9F4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801277 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801273 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801272 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801271 Y Aedes aegypti Yellow fever mosquito  Aaeg\Hsp70Cb Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL801278 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ014283 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011082 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011083 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011081 Y
Orthologs in non-Dipteran Insects (EOG6B8H85)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002344 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002346 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003712 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008763 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI067751 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI007961 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI000903 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001597 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI009117 Y Bombyx mori Silkmoth  Bmor\BGIBMGA014536 Y Pediculus humanus Human body louse  Phum\PHUM126800 Y Tribolium castaneum Red flour beetle  Tcas\GLEAN_05094 Y Tribolium castaneum Red flour beetle  Tcas\hsp68b Y Tribolium castaneum Red flour beetle  Tcas\TC000188 Y Tribolium castaneum Red flour beetle  Tcas\hsp68b Y Tribolium castaneum Red flour beetle  Tcas\TC010172 Y
Orthologs in non-Insect Arthropods (EOG6K98TB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_256736 Y Daphnia pulex Water flea  Dpux\JGI_V11_302856 Y Daphnia pulex Water flea  Dpux\Rabgap1 Y Ixodes scapularis Deer tick  Isca\ISCW011425 Y Ixodes scapularis Deer tick  Isca\ISCW024910 Y
Orthologs in non-Arthropod Metazoa (EOG609123)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\F44E5.4 Y Caenorhabditis elegans Nematode  Cele\F44E5.5 Y Caenorhabditis elegans Nematode  Cele\hsp-70 Y Caenorhabditis elegans Nematode  Cele\hsp-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009477gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016500gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005808gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014864gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009164gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009165gn Y Danio rerio Zebrafish  Drer\hsp70 Y Danio rerio Zebrafish  Drer\zgc:63663 Y Danio rerio Zebrafish  Drer\hspa8l Y Danio rerio Zebrafish  Drer\si:ch211-199o1.2 Y Danio rerio Zebrafish  Drer\hspa8 Y Xenopus tropicalis Western clawed frog  Xtro\hspa1l Y Xenopus tropicalis Western clawed frog  Xtro\hspa1a Y Xenopus tropicalis Western clawed frog  Xtro\hspa8 Y Xenopus tropicalis Western clawed frog  Xtro\hspa1b Y Xenopus tropicalis Western clawed frog  Xtro\hsp70 Y Xenopus tropicalis Western clawed frog  Xtro\hspa2 Y Gallus gallus Domestic chicken  Ggal\HSP70 Y Gallus gallus Domestic chicken  Ggal\HSPA8 Y Mus musculus House mouse  Mmus\Hspa1a Y Mus musculus House mouse  Mmus\Hspa1l Y Mus musculus House mouse  Mmus\Hspa1b Y Mus musculus House mouse  Mmus\Hspa2 Y Mus musculus House mouse  Mmus\Hspa8 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000009920 Y Rattus norvegicus Norway rat  Rnor\Hspa8 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000034066 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022105 Y Rattus norvegicus Norway rat  Rnor\Hspa1a Y Rattus norvegicus Norway rat  Rnor\Hspa2 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029072 Y Homo sapiens Human  Hsap\HSPA8 Y Homo sapiens Human  Hsap\HSPA2 Y Homo sapiens Human  Hsap\ENSG00000204388 Y Homo sapiens Human  Hsap\HSPA1A Y Homo sapiens Human  Hsap\HSPA1L Y Homo sapiens Human  Hsap\HSPA6 Y
Human Orthologs (6)
Gene OMIM HGNC Hsap\HSPA8     Hsap\HSPA2     Hsap\ENSG00000204388     Hsap\HSPA1A     Hsap\HSPA1L     Hsap\HSPA6
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	32997 y1 sc* v1; P{TRiP.HMS00797}attP2 33948 y1 sc* v1; P{TRiP.HMS00900}attP2
Genomic Clones ( 2 )
	BACR02G11 BACR28I14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 52 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT29796 LP03941 LP05203 LP08776 LP11008 LP11561 LP13742 LP23554 RE48592
Other clones	DPiM_LP05203 DPiM_RE48592
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT18530 AT25953 AT26254 GH24523 GM03919 GM10252 GM12929 GM15126 GM15741 GM24465 GM25395 GM26243 LD11110 LD39317 LP02551 LP04534 LP09910 LP22875 SD12004 SD19706 SD24418 UT01572
Other clones	50892 157253 214477.35 214477.36 280361 CK01.50B.H1 CK01.54C.E3 Dmel_neb_002337_0915_0342 EC054721 EK041629 EK044218 EK140537 EK176729 EK188006 EK260321 EK305248 EP05927 EP09235 IP16292
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013278 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 48582
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Flies with no copies of the Hsp70 genes (Hsp70Aa, Hsp70Ab, Hsp70Ba, Hsp70Bb, Hsp70Bbb and Hsp70Bc) are unable to survive a severe heat shock. These flies show a lengthened heat-shock response and developmental delay following a non-lethal heat shock. (Gong and Golic, 2006) Flies with no copies of the Hsp70 genes (Hsp70Aa, Hsp70Ab, Hsp70Ba, Hsp70Bb, Hsp70Bbb and Hsp70Bc) are viable and fertile. (Gong and Golic, 2004) Some Drosophila strains, including that sequenced in the Drosophila genome project have three, not two, tandemly repeated Hsp70 genes at 87C1 (in addition to a reverse orientation Hsp70 gene, Hsp70Ba, approximately 40kb upstream). The distal-most gene of the three tandem copies is Hsp70Bc. The inner two tandem copies are Hsp70Bb and Hsp70Bbb. (Bettencourt, 2002.1.16) The D.melanogaster strain used for sequencing the genome contains an additional, third tandem copy of an Hsp70 gene at 87C as well as the two tandemly repeated copies (Hsp70Bb and Hsp70Bc) which are found in many strains. This additional copy is named "Hsp70Bbb" (see FBrf0137034 and Bettencourt, 2002.1.16, personal communication to FlyBase). (FlyBase Genome Annotators, 2002-2003) New annotation (CG31359) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Nucleosomes are not required for polymerase pausing on the Hsp70Bb promoter. (Benjamin and Gilmour, 1998) Variation in copy number of Hsp70Bb and Hsp70Bc demonstrates while extra Hsp70 provides additional protection against the immediate damage from heat stress, abnormally high concentrations can decrease growth, development and survival to adulthood. (Krebs and Feder, 1997) Natural variation in Hsp70 expression is analysed to study thermotolerance. (Krebs and Feder, 1997) The rate and intensity of Hsp70Bb protein expression has been compared with tissue damage after heat shock. (Krebs and Feder, 1997) Expression is rapidly induced in the testis after heat stress. Activation of Hsp70 in testes and heads necessitates the presence of a functional Hsf. (Michaud et al., 1997) There is significant variation among 74 different 2nd chromosome lines and 70 different 3rd chromosome lines in response to heat shock, measured by mRNA accumulation. (Otsuka et al., 1997) Species-wide analysis demonstrates a characteristic of heat-shock-inducible genes is the absence of introns. (Silke, 1997) Construct analysis reveals deletion of the TATA box or the upstream region greatly inhibits transcription under heat shock conditions. Analysis of DNase I hypersensitivity reveals non-histone protein interactions on the mutant promoters. Paused polymerase and Tbp on the Hsp70Bb promoter is detected in intact salivary glands, but not on a promoter lacking the TATA box. (Weber et al., 1997) d(GA.TC)n sequences can be found in the promoters of Hsp26 and the Hsp70 genes. In vitro assembly of mononucleosomes into short DNA fragments carrying d(GA.TC)n sequences of different lengths is very efficient. Nucleosome assembly is inhibited strongly when the d(GA.TC)n sequence forms a triple-stranded conformation. Triplex formation requires partial destabilisation of the nucleosome. Results indicate nucleosome assembly and triplex formation are competing processes. (Espinas et al., 1996) Variation in copy number of Hsp70Bb and Hsp70Bc affects Hsp70 protein concentration in whole larvae and pupae, which in turn affects their tolerance of natural thermal stress and, potentially, their fitness. (Feder et al., 1996) Minimal secondary structure is a key determinant of preferential translation during heat shock and the specific location of a cap-proximal portion of the leader influences initiation and a cap-independent mode of translation in unlikely. Heat shock reduces the capacity to unwind 5'-UTR secondary structure, allowing only mRNAs with minimal 5'-UTR secondary structure to be efficiently translated. (Hess and Duncan, 1996) RNA polymerase pauses on the Hsp70Bb and His3 promoters in a nuclear extract derived from embryos. Analysis of the transcripts produced from the Hsp70Bb promoter indicates that more than 50% of the polymerases that initiate fail to elongate beyond 40 nucleotides. Permanganate assay also detects a paused polymerase. (Li et al., 1996) Chromosome homologies of Muller's element D (J chromosome in the Paleartic species and XR chromosome arm in Nearctic species) and of element E (O chromosome in the Paleartic species and 2 chromosome in Nearctic species) have been confirmed by single copy probes in the species of the obscura group and in D.melanogaster. (Segarra et al., 1996) Sodium salicylate induces activation of Hsf binding activity in salivary gland cells and Schneider SL2 tissue cells. Puffing of heat shock gene loci occurs in salivary glands but Hsp70 transcription is not induced suggesting puffing and transcription are separable events. (Winegarden et al., 1996) The in vitro binding of Hsf protein to the promoter region of a number of heat shock genes has been analysed. (Fernandes et al., 1995) UV cross linking technique has been used to study the in vivo distribution of Trl protein on Hsp70 and Hsp26. Prior to heat shock Trl protein is associated with the promoter regions of the uninduced Hsp70 and Hsp26 genes. Upon heat shock induction Trl protein is recruited to their transcription units with its distribution coincident with that of RNA polymerase II. (O'Brien et al., 1995) Analysis of transgenic lines containing altered Hsp70Bb promoters (fused to reporter gene Yp1), analysed for their ability to bind Hsf protein, indicates that at least three promoter sequences facilitate the binding of Hsf to chromatin, including binding sites for Trl, Tbp and RNA polymerase II. (Shopland et al., 1995) The molecular architecture of the Hsp70Bb promoter has been analysed by genomic footprinting. (Weber and Gilmour, 1995) RNA levels do not increase with age, so the observed increase in protein levels is due to post-transcriptional regulation. Aging-specific expression may be a result of oxidative damage. (Wheeler et al., 1995) Analysis of Hsp70Bb-Ecol\lacZ constructs in which the spacing between HSEs (heat shock elements) I and II, and between HSEII and the TATA box are altered suggests that Hsf binds cooperatively to the Hsp70Bb promoter. (Amin et al., 1994) Heat-induced alterations in RNA-binding proteins do not mediate preferential translation of heat stress mRNAs or repression of normal mRNAs. (Hess and Duncan, 1994) Synthesis of heat shock proteins is inhibited by both short-chain fatty acids and their corresponding alcohols, compounds which have no observable effect on histone acetylation. (Munks and Turner, 1994) Gene contains an RNA polymerase II complex which pauses after synthesis of a short transcript. In vivo ultraviolet crosslinking techniques demonstrate phosphorylation of the carboxy terminal domain (CTD) of the large subunit of RNA polymerase II could either regulate the transition of polymerase from a paused to an elongated complex or be a consequence of the transition from paused to elongated. (O'Brien et al., 1994) The TFIID complex interacts with the promoter of Hsp70Bb making contacts at the TATA element, initiator, +18 and +28 regions. The complex recognises a sequence within the context of the promoter region that corresponds to an initiator consensus sequence. (Purnell et al., 1994) Chromosome staining reveals that Trl and heat shock transcription factors (HSF) colocalises at Hsp70Bb. (Tsukiyama et al., 1994) The 87A7 Hsp70B region is bordered on the proximal and distal sides by two special chromatin structures, scs and scs'. An enhancer blocking assay based on the w gene demonstrates that the two nuclease hypersensitive regions that define part of the scs chromatin structure are essential for the blocking activity. The DNA sequence spanning the nuclease resistant core located between the two hypersensitive regions is dispensable. (Vazquez and Schedl, 1994) In vivo crosslinking studies demonstrate that endogenous eve and ftz protein significantly interacts with the promoter region, although this is not an expected target gene. (Walter et al., 1994) The transcription of heat shock proteins, except Hsp83, is independent of the p200 subunit of initiation factor eIF-4F, eIF-4G. (Zapata et al., 1994) RNA polymerase distribution on uninduced Hsp70 genes has been compared with the distribution of RNA polymerase on DRB (5,6,-Dichloro-1-β-D-ribofuranosylbenzimidazole)-inhibited induced Hsp70 genes. (Giardina and Lis, 1993) In vivo UV cross-linking and nuclear run-on assays shows that RNA polymerase II density on the Hsp70Bb gene is rapidly increased by heat shock. (O'Brien and Lis, 1993) Hsp70 proteins are of prime importance to heat tolerance. (Parsell et al., 1993) TATA complex formation on the Hsp70Bb core promoter shows sequence dependence at the TATA element, at the transcription start site and further downstream. Hydroxyl radical interference assays show that these sequences contribute to the TATA complex. The TATA complex contains Tbp. Methylation interference shows that protein contact at the TATA element is in the minor groove. Similar interactions contribute to TATA complexes formed on the Hsp26 and His4 promoters. (Purnell and Gilmour, 1993) Members of the hsc70 gene family (heat shock cognate genes) that reside within the same intracellular compartment in different organisms share greater amino acid identity than hsc70 proteins from the same organism but different organelles. This pattern of conservation indicates specialisation of hsc70 function. (Rubin et al., 1993) Identified in 2D gels of CMW W2 wing imaginal disc cell proteins. (Santaren et al., 1993) Nascent chain nuclear run-on assays in KC161 cells reveal different responses to heat shock for different genes. Transcription of His1 is severely inhibited under mild heat shocks, of Act5C decreases proportionally with increasing temperature while that of the core histone genes or the heat shock cognates is repressed only under extreme heat shock. In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range. Hsrω is transcribed at a very high rate under non-heat shock conditions, and its response to elevated temperatures is different from that of the protein coding heat shock genes. (Vazquez et al., 1993) Expression of transgenes carrying Hsp70Bb and Hsp70Bc demonstrate that at early embryonic stages the accumulation of Hsp70 is a rate-limiting factor in the acquisition of thermotolerance. Genetic manipulation can improve stress tolerance. (Welte et al., 1993) Hsp70 promoter driven expression in heat induced and non-heat induced conditions is more efficient in D.melanogaster embryos than in L.cuprina embryos. (Atkinson and O'Brochta, 1992) The interaction of Top1 and Top2 gene products with transcriptionally active and inactive Hsp70B has been compared. Topoisomerase I binding sites are found in the transcribed portions of the Hsp70B gene, and only when Hsp70B is transcriptionally active. Topoisomerase II binding to Hsp70B sequences occurs on both transcriptionally active and inactive chromatin. An unusual type of topoisomerase II binding site is associated with the 5' ends of inactive Hsp70 gene, suggesting that this enzyme may be associated with repression of gene transcription. (Kroeger and Rowe, 1992) Using Yp1 reporter gene constructs distal and proximal regions of the Hsp70 promoter have been identified that are required for formation of paused RNA polymerase II, the polymerase is transcriptionally engaged but paused about 25 nucleotides from the start site of Hsp70. (Lee et al., 1992) The effect of aging on the expression of the "Hsp70" genes (Hsp70Aa, Hsp70Ab, Hsp70Ba, Hsp70Bb and Hsp70Bc) has been studied. (Niedzwiecki et al., 1991) Pausing of RNA polymerase II on the Hsp70Bb gene is not restricted to uninduced cells. A number of paused and elongating RNA polymerase II molecules are detected at the 5' end of Hsp70Bb when transcribed at intermediate levels. Elongation of polymerase from the pause remains the rate limiting step in transcription. (O'Brien and Lis, 1991) Translation of Hsp70 mRNAs and to a lesser extent the mRNAs for the small heat shock proteins is almost independent of eIF-4E. (Zapata et al., 1991) Induction of heat shock protein after metal ion exposure is studied. (Bournias-Vardiabasis et al., 1990) A TATA-dependent protein-DNA complex is found in fractionated embryonic nuclear extracts. DNase I footprint analysis identifies polypeptides (including Taf12, Tbp and RpII140) that require the Hsp70Bb TATA element for binding to regions of the Hsp70Bb promoter. (Gilmour et al., 1990) Analysis of Hsp70-Adh and Hsp70-Ecol\CAT constructs suggests that the Hsp70 3' untranslated region is required for the instability of Hsp70 mRNA seen at normal growth temperatures and for the selective degradation of Hsp70 mRNA seen during recovery from heat shock. (Petersen and Lindquist, 1989) Nuclear run-on experiments investigate the interaction between RNA polymerase II and the uninduced Hsp70Bb promoter. (Rougvie and Lis, 1988) Deletion analysis of the 5' side of Hsp70Bb demonstrates that two nuclease-hypersensitive sites in chromatin exist upstream of Hsp70Bb. It is proposed that the hypersensitive sites are generated through the binding of a protein that renders the flanking sequence more accessible to nucleases, perhaps by preventing normal chromatin packaging. (Costlow et al., 1985) Studies demonstrate that heat shock puffs at the site of a construct insertion can be formed if the inserted segment contains a functional heat shock promoter and the active promoter is joined to long transcription units. (Simon et al., 1985) Sequences located 5' to Sgs7, Sgs8, Sgs3, the Hsp70 genes at 87A and 87C and the copia coding region are similar to the sequence at -405 from Sgs4. (Shermoen and Beckendorf, 1982) initiates the heat-shock response. A restricted subset of genes, the Hsp genes, is activated and the majority of transcription and translation is shut down. However, mitochondrial- and histone-gene activities persist (Spradling, Pardue and Penman, 1977). This response follows a pulse of 36oC to 40oC; treatments above 40oC inhibit all activity and lead to death; treatments of 30oC-35oC induce heat-shock-protein synthesis without repressing normal protein synthesis (Tissieres, Mitchell and Tracy, 1974). Similar response inducible by other stressful treatments. The response may be elicited at all stages of the life cycle and in cultured cells. Stage specific phenocopies result from heat shocking early stages of Drosophila development (Mitchell and Petersen, 1982). In polytene cells existing puffs regress and a novel group quickly appears at 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D (Ashburner, 1970; Tissieres, Mitchell and Tracy, 1974). Activation of transcription of Hsp genes apparently involves the sequential binding of two or more protein factors in vicinity of TATA box (Wu, 1984). Binding sites for these proteins are multiple short upstream sequence elements called HSEs or heat shock consensus elements (Pelham, 1982; Xiao and Lis, 1988). Polymerase II dissociates from most chromosome regions and accumulates at the new puff sites (Bonner and Kerby, 1982). 3H-uridine incorporation ceases at its usual positions and commences at new puff sites. Preexisting polysomes disaggregate and within a few minutes a new population of polysomes appears containing newly transcribed mRNA; this RNA hybridizes to some of the heat-shock puffs. The effects of heat shock may be abrogated to some degree by pretreatment with a pulse of a slightly lower temperature (Mitchell, Moller, Petersen and Lipps-Sarmiento, 1979; Peterson and Mitchell, 1981). For reviews of the heat-shock response see Ashburner and Bonner (1978).   One of five structural genes (in two clusters, Hsp70A, Hsp70B) that code for the 70,000 dalton heat-shock protein (HSP70), the most abundant of the heat-shock proteins. Hsp70B usually includes three HSP70 encoding genes (Hsp70Ba (proximal), Hsp70Bb (middle), Hsp70Bc (distal)) (Holmgren et al., 1979) with slightly different restriction maps (Artavanis-Tsakonas et al., 1978). HSP70 returns to preshock levels more rapidly than other heat-shock proteins following return to 25oC (DiDomenico, Bugaisky and Lindquist, 1982). The protein becomes concentrated in nuclei during heat shock; disperses to cytoplasm during recovery; returns to nucleus upon further heat shock (Velazquez and Lindquist, 1984). Appears not to be expressed in the testis in response to heat-shock stimulation (Bonner, Parks, Parker-Thornberg, Mortin and Pelham, 1984). Deletion of either Hsp70A or Hsp70B does not eliminate the HSP70 heat-shock response; simultaneous deletion of both does (Ish-Horowicz et al., 1979).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080188 RefSeq (Proteins) • NP_524927 DDBJ / EMBL / GenBank • AAD15226 AAG26905 AAG26906 AAG26907 AAG26908 AAG26909 AAG26910 AAG26911 AAK30233 AAK30234 AAK30235 AAK30236 AAK30237 AAK30238 AAK30239 AAK30240 AAN13546 AAQ75092 AF295951 AF295952 AF295953 AF295954 AF295955 AF295956 AF295957 AF350476 AF350477 AF350478 AF350479 AF350480 AF350481 AF350482 AF350483 AI257020 AY370940 CS162814 J01104 K01861 M10229 Entrez Gene - A searchable database of RefSeq genes. • 48582 UniProtKB/Swiss-Prot • Q9BIS2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Heat shock protein 70, conserved site (IPR018181) Heat shock protein 70 family (IPR013126)
Linkouts
	BioGRID - A database of protein and genetic interactions • 71512 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0013278 DroID - A comprehensive database of gene and protein interactions. • FBgn0013278 DRSC - Results from RNAi screens. • FBgn0013278 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013278 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6489-RA;CG31449-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013278 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 48582 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 48582 modMine - Data generated by the modENCODE project. • FBgn0013278
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	CG31359 (Hughes et al., 2008, Neal et al., 2006, Ni et al., 2010.12.1) dhsp70 (Rabindran et al., 1994) dHsp70 (Bell et al., 2007) DMHSP7D1 (Papadimitriou et al., 1998) Dm-hsp70 (Hernandez et al., 2004) Hsp70 (Klose et al., 2005, Baker and Russell, 2009, Auluck et al., 2005, Scholz et al., 2005, Ahmed and Duncan, 2004, Jennings et al., 2004, Zhai et al., 2004, Gruntenko et al., 2003, Morrow et al., 2004, Temme et al., 2004, Marsh and Thompson, 2004, Sathyanarayanan et al., 2004, Jin et al., 2003, Canamasas et al., 2003, Norry and Loeschcke, 2003, Rutherford, 2003, Silbermann and Tatar, 2000, Sejerkilde et al., 2003, Krebs, 1999, Lakhotia et al., 2002, Auluck and Bonini, 2003, Caletka and Fry, 2003, Shaw et al., 2002, Auluck et al., 2002, Rajendra et al., 2001, Sorensen and Loeschcke, 2001, Bulgheresi et al., 2001, Mok et al., 2001, Zatsepina et al., 2001, Kelty and Lee, 2001, de Carcer et al., 2001, Ekengren and Hultmark, 2001, Wu et al., 2001, Krebs et al., 2001, Andrulis et al., 2000, Gunawardena and Rykowski, 2000, White et al., 1999, Feder, 1999, Elefant and Palter, 1999, Jazwinski, 1998, Dahlgaard et al., 1998, Wang and Lindquist, 1998, Cossins, 1998, Krebs and Feder, 1998, Gu, 1998, Ashburner et al., 1998, Krebs et al., 1998, Michaud et al., 1997, Krebs and Feder, 1997, Krebs and Feder, 1997, Krebs and Feder, 1997, Espinas et al., 1996, Winegarden et al., 1996, Hess and Duncan, 1996, Kingston et al., 1996, Song et al., 1995, Engel and Cornelius, 1995, Duncan, 1995, Weber and Gilmour, 1995, Li and Duncan, 1995, O'Brien et al., 1995, Gehring and Wehner, 1995, Lis and Wu, 1994, Fernandes et al., 1994, Parsell et al., 1993, Evgen'ev and Denisenko, 1990, Papaconstantinou et al., 2006, Behr et al., 2006, Shilova et al., 2006, Fernandez-Funez et al., 2007, Sengupta and Lakhotia, 2006, Tenney et al., 2006, Smith et al., 2008, Ahrens et al., 2007, Zobeck et al., 2010, Hanyu-Nakamura et al., 2008, Eissenberg et al., 2007, Morettini et al., 2011, Bonini, 2002, Casas-Tinto et al., 2008, Mito et al., 2007, Huen and Chan, 2005, Brandt and Corces, 2008, Newman et al., 2005, Yao et al., 2007, Udaka et al., 2010, Ni et al., 2008, Posgai et al., 2009, Ahamed et al., 2010, Iijima-Ando et al., 2005, Cai et al., 2008, Gilchrist et al., 2008, Takahashi et al., 2011, Bettencourt and Feder, 1999, Lerman and Feder, 1999, Sørensen et al., 2009, Chopra et al., 2009, Kalosaka et al., 2006, Nielsen et al., 2005, Overgaard et al., 2005, Kristensen et al., 2003, Liévens et al., 2008, Cobreros et al., 2008, Piacentini et al., 2009, Tian et al., 2010, Bartkowiak et al., 2010, Hrizo and Palladino, 2010, Kwon et al., 2010, Zobeck et al., 2010, Jensen et al., 2010, Krebs and Holbrook, 2001, Neal et al., 2006, Fujikake et al., 2008, Gupta et al., 2005, Duncan, 2005, McLear et al., 2008, Rezaval et al., 2007, Duncan, 2008, Bhargav et al., 2008, Schneiderman et al., 2012, Calabria et al., 2012, Fredriksson et al., 2012, Ardehali et al., 2009) hsp70 (Adelman et al., 2005, Wang et al., 2004, Matsumoto and Hirose, 2004, Landis et al., 2004, Smith et al., 2004, Ni et al., 2004, Saunders et al., 2005, Venkaiah et al., 2004, Lehmann, 2004, Viswanathan et al., 2003, Helfand and Rogina, 2003, Goodman et al., 2004, Tatar and Yin, 2001, Saunders et al., 2003, Kellum, 2003, Nazir et al., 2003, Shaw and Franken, 2003, Feder, 2003, Geyer et al., 2000, Mahowald, 2001, Luckinbill and Foley, 2000, Wimmer, 2003, Gilmour, 2003.4.18, Aigaki et al., 2002, Tulin and Spradling, 2003, Andrulis et al., 2002, Gong and Golic, 2003, Goodman et al., 2003, Kazantsev et al., 2002, Lakhotia and Prasanth, 2002, Herold et al., 2001, Gatfield et al., 2001, Biggin, 2001, Crevel et al., 2001, Christoffels et al., 2000, Badenhorst and Wu, 2001, Leach et al., 2000, Farkas et al., 2000, Yueh and Schneider, 2000, Lakhotia et al., 1999, Tatar, 1999, Feder, 1999, Hirayoshi and Lis, 1999, Bonini, 1999, Wu et al., 1998, Lis, 1998, Karunanithi et al., 1999, Mannervik, 1999, Sah et al., 1999, King and Tower, 1999, Katsani et al., 1999, Carr and Biggin, 1999, Zhao and Eggleston, 1999, Wilkins and Lis, 1999, Agianian et al., 1999, Nevo, 1998, Liang and Biggin, 1998, Gdula et al., 1998, Yiangou et al., 1997, Wu and Fallon, 1997, Wilkins and Lis, 1998, Vernick and McCutchan, 1998, John and Workman, 1998, Curtsinger et al., 1997, Marino et al., 1998, Sun et al., 1998, Liang and Biggin, 1998, Gregory and Horz, 1998, Benjamin and Gilmour, 1998, Martinez-Balbas et al., 1998, Elgin et al., 1997, Greenblatt, 1997, Burke and Kadonaga, 1997, Wilkins and Lis, 1997, Finch and Tanzi, 1997, Otsuka et al., 1997, Tower, 1996, Shopland and Lis, 1996, Li et al., 1996, Sandaltzopoulos et al., 1994, Segarra et al., 1996, O'Brien et al., 1994, Wheeler et al., 1996, Fernandes et al., 1995, Elefant and Palter, 1995, Wheeler et al., 1995, Shopland et al., 1995, Rasmussen and Lis, 1995, Granok et al., 1995, Munks and Turner, 1994, Parsell and Lindquist, 1993, Zapata et al., 1994, Krumm et al., 1993, Amin et al., 1994, Fuller, 1993, Soeller et al., 1993, Lakhotia et al., 1993, Giardina and Lis, 1993, O'Brien and Lis, 1993, Purnell and Gilmour, 1993, Kroeger et al., 1993, Molto et al., 1993, Heikkila, 1993, Papaceit and Juan, 1993, Vazquez et al., 1993, Morse, 1992, Fleming et al., 1992, Atkinson and O'Brochta, 1992, Lee et al., 1992, Feder et al., 1992, Pauli et al., 1992, Read and Manley, 1992, Becker et al., 1991, O'Brien and Lis, 1991, Niedzwiecki et al., 1991, Zapata et al., 1991, Bendena et al., 1991, Sorger, 1991, Spencer and Groudine, 1990, Helms and Rottman, 1990, Gilmour et al., 1990, Perkins et al., 1990, Karlin et al., 1990, Ornelles and Penman, 1990, Elgin, 1990, Ohkuma et al., 1990, Menke and Petersen, 1989, Petersen and Lindquist, 1989, Petersen and Lindquist, 1988, Buszczak and Spradling, 2006, Kim and Lis, 2005, Graves and Tamkun, 2006, Halfon and Arnosti, 2007, Yao et al., 2006, Boeke et al., 2011, Wu et al., 2005, Piel et al., 2008, Ullah et al., 2007, Ivaldi et al., 2007, Smith et al., 2008, Melnikova et al., 2010, Siddique et al., 2009, Murawska et al., 2011, Behm-Ansmant et al., 2007, Kurshakova et al., 2007, Singh et al., 2009, Hsu et al., 2008, Bettencourt and Feder, 2000, Grover et al., 2009, Zhao et al., 2005, Li et al., 2001, Poels et al., 2004, Yang and Tower, 2009, Tombácz et al., 2009, Chan et al., 2011, Vazquez-Pianzola et al., 2007, Kopytova et al., 2010, Kotova et al., 2010, Juge et al., 2010, Weisbrot et al., 2003, Bhole et al., 2004, Ardehali et al., 2011, Ghosh et al., 2011, Lebedeva et al., 2007, Lee et al., 2008, Grover et al., 2008, Siddique et al., 2008, Gupta et al., 2007, Pankotai et al., 2010, Sharma et al., 2012, Siddique and Afzal, 2012) HSP70 (Gerber et al., 2005, Aalfs and Kingston, 2000, Bondinas et al., 2002, Foley and Luckinbill, 2001, Bondinas et al., 2001, Driscoll and Gerstbrein, 2003, Zoghbi and Botas, 2002, Garbuz et al., 2002, Mitchell-Olds and Knight, 2002, Takada et al., 2000, Warrick et al., 1999, Mantovani, 1999, Takahashi and Fujiwara, 1999, Karlin and Brocchieri, 1998, Ogbourne and Antalis, 1998, Nover and Scharf, 1997, Jedlicka et al., 1997, Singh and Lakhotia, 1995, Hess and Duncan, 1994, Rubin et al., 1993, Warrick and Boyd, 2006, Chartier et al., 2006, Papaconstantinou et al., 2005, Farny et al., 2008, Hurt et al., 2009, Folk et al., 2006, Sakaguchi et al., 2008) Hsp 70 (Zuckerkandl, 1997) hsp-70 (Luis et al., 1990, Belikov et al., 1990) hsp70 87C (Shermoen and Beckendorf, 1982) Hsp70(87C) (Henikoff, 1990) Hsp70A7d (Maroni, 1994) hsp70B (Gong and Golic, 2004, Huang et al., 2008) Hsp70B (Ranz et al., 2001, Whitfield, 2001.1.4, Ranz et al., 2001, Ashburner, 2000.10.30, Ruiz et al., 1997, Ranz et al., 1997, Buchon et al., 2009, Meisel, 2009, Chen et al., 2008) hsp70Bb (Roman, 2004, Lerman et al., 2003, Bettencourt, 2002.1.16, Bettencourt et al., 2002, Bettencourt and Feder, 2001, Bettencourt et al., 1999, Bettencourt and Feder, 2002, Lerman and Feder, 2005) Hsp70Bb (Shilova et al., 2006, Kuan et al., 2009, Nikolaidis and Nei, 2004, Dorus et al., 2006, Shieh and Bonini, 2011, Bettencourt et al., 2008, Azad et al., 2009, Zeitlinger et al., 2007, Haney and Feder, 2009, Bergman et al., 2006, Tian et al., 2010, Xi et al., 2008, Takahashi et al., 2010, Cernilogar et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Paré et al., 2012) Hsp70Bbc (Takahashi et al., 2001) Hsp70Bc (Maside et al., 2002, Chen et al., 2010) unnamed (Marti-Subirana and Holmgren, 1997.2.18)
Name Synonym	87C locus (Lakhotia et al., 1990) heat-shock protein 70 (Iijima-Ando et al., 2005, Farny et al., 2008) Heat-shock-protein-70Bb   Protein 70 (Bournias-Vardiabasis et al., 1990)
Secondary FlyBase IDs
	FBgn0001232 FBgn0051359
References ( 491 )
Generate a list of	All references relating to this gene ( 491 )
List References by type	Research paper  ( 369 ) Supplementary material  ( 8 ) Review  ( 58 ) Retraction  ( 1 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 29 ) FlyBase analysis  ( 2 ) Book review  ( 1 ) Letter  ( 2 ) Conference report  ( 2 ) Stock list  ( 1 ) Book  ( 1 ) DNA/RNA sequence record  ( 4 )
Recent research papers ( 22 )
	Sørensen et al., 2013, J. Exp. Biol. 216(5): 809--814 Cellular damage as induced by high temperature is dependent on rate of temperature change - investigating consequences of ramping rates on molecular and organismal phenotypes in Drosophila melanogaster. [FBrf0220807] Calabria et al., 2012, J. Evol. Biol. 25(4): 691--700 Hsp70 protein levels and thermotolerance in Drosophila subobscura: a reassessment of the thermal co-adaptation hypothesis. [FBrf0217786] Coléno-Costes et al., 2012, PLoS Genet. 8(10): e1003006 New partners in regulation of gene expression: the enhancer of trithorax and polycomb corto interacts with methylated ribosomal protein l12 via its chromodomain. [FBrf0219702] Defays and Bertoli, 2012, Alcohol 46(8): 737--745 Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster. [FBrf0220080] Fredriksson et al., 2012, Aging Cell 11(4): 634--643 Effects of aging and reproduction on protein quality control in soma and gametes of Drosophila melanogaster. [FBrf0218923] Fuda et al., 2012, Mol. Cell. Biol. 32(17): 3428--3437 Fcp1 Dephosphorylation of the RNA Polymerase II C-Terminal Domain Is Required for Efficient Transcription of Heat Shock Genes. [FBrf0219151] Landis et al., 2012, Aging 4(11): 768--789 Gene expression changes in response to aging compared to heat stress, oxidative stress and ionizing radiation in Drosophila melanogaster. [FBrf0220226] Paré et al., 2012, PLoS ONE 7(5): e36254 The functions of grainy head-like proteins in animals and fungi and the evolution of apical extracellular barriers. [FBrf0218280] Schneiderman et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(48): 19721--19726 Nucleosome-depleted chromatin gaps recruit assembly factors for the H3.3 histone variant. [FBrf0220071] Sharma et al., 2012, J. Hazard. Mater. 221-222: 275--287 Organochlorine pesticide, endosulfan induced cellular and organismal response in Drosophila melanogaster. [FBrf0218375] Siddique and Afzal, 2012, J. Pharmacol. Pharmacother. 3(2): 188--190 Protective effects of apigenin against methyl methanesulfonate induced hsp70 expression in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg(9). [FBrf0218432] Ardehali et al., 2011, EMBO J. 30(14): 2817--2828 Drosophila Set1 is the major histone H3 lysine 4 trimethyltransferase with role in transcription. [FBrf0214333] Boeke et al., 2011, PLoS ONE 6(6): e20761 The RNA Helicase Rm62 Cooperates with SU(VAR)3-9 to Re-Silence Active Transcription in Drosophila melanogaster. [FBrf0213941] Cernilogar et al., 2011, Nature 480(7377): 391--395 Chromatin-associated RNA interference components contribute to transcriptional regulation in Drosophila. [FBrf0216979] Chan et al., 2011, Hum. Mol. Genet. 20(9): 1738--1750 Expanded polyglutamine domain possesses nuclear export activity which modulates subcellular localization and toxicity of polyQ disease protein via exportin-1. [FBrf0214367] Ghosh et al., 2011, Mol. Cell. Biol. 31(20): 4232--4243 Negative Elongation Factor Accelerates the Rate at Which Heat Shock Genes Are Shut off by Facilitating Dissociation of Heat Shock Factor. [FBrf0215827] Morettini et al., 2011, Nucleic Acids Res. 39(8): 3103--3115 The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization. [FBrf0213515] Murawska et al., 2011, PLoS Genet. 7(7): e1002206 Stress-Induced PARP Activation Mediates Recruitment of Drosophila Mi-2 to Promote Heat Shock Gene Expression. [FBrf0214629] Sayal et al., 2011, Fly 5(1): 47--52 Optimization of reporter gene architecture for quantitative measurements of gene expression in the Drosophila embryo. [FBrf0213005] Shieh and Bonini, 2011, Hum. Mol. Genet. 20(24): 4810--4821 Genes and pathways affected by CAG-repeat RNA-based toxicity in Drosophila. [FBrf0216738] Takahashi et al., 2011, PLoS ONE 6(4): e17295 Environmental Stress-Dependent Effects of Deletions Encompassing Hsp70Ba on Canalization and Quantitative Trait Asymmetry in Drosophila melanogaster. [FBrf0213649] Teves and Henikoff, 2011, Genes Dev. 25(22): 2387--2397 Heat shock reduces stalled RNA polymerase II and nucleosome turnover genome-wide. [FBrf0216728] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...