FlyBase Gene Report: Dmel\nSyb

adult brain cell body rind

photoreceptor cell

(DiAntonio et al., 1993)

• neuronal cell body

(DiAntonio et al., 1993)

cell body of adult optic lobe

(DiAntonio et al., 1993)

adult ventral nervous system

• neuronal cell body

(DiAntonio et al., 1993)

embryonic stage 13 -- 17

presumptive embryonic/larval peripheral nervous system

(DiAntonio et al., 1993)

presumptive embryonic/larval central nervous system

(DiAntonio et al., 1993)

embryonic stage 13 -- 16

embryonic antennal sense organ

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(DiAntonio et al., 1993)

Additional Descriptive Data

n-syb transcripts are first detected in 9-12hr embryos on northern blots and are detected at all subsequent stages tested. The two transcripts are present in roughly equal amounts until adulthood at which point the 3.0kb transcript predominates. n-syb transcripts are more abundant in adult head than body.

(DiAntonio et al., 1993)

n-syb transcripts are first detected in embryos at stage 13 and are restricted to the nervous system. They become quite abundant in both the central and peripheral nervous sytems. In adults, transcripts are detected in the cell bodies of the visual system, in the central brain and in the thoracic ganglia.

(DiAntonio et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

bouton

(Broadie et al., 1995)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

neuromuscular junction

(Broadie et al., 1995)

synapse

(Broadie et al., 1995)

embryonic stage

synapse of neuromuscular junction

(Deitcher et al., 1998)

neuromuscular junction of abdominal 1 ventral longitudinal muscle 3

(Deitcher et al., 1998)

neuromuscular junction of abdominal 1 ventral longitudinal muscle 4

(Deitcher et al., 1998)

intersegmental nerve

(Deitcher et al., 1998)

ventral nerve cord

(Deitcher et al., 1998)

segmental nerve

(Deitcher et al., 1998)

ventral nerve cord commissure

(Deitcher et al., 1998)

lamina

(Hiesinger et al., 2001)

photoreceptor cell

(Hiesinger et al., 2001)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

n-syb protein staining is strong in the ventral nerve cord in embryos and is faint in axon commissures and segmental and intersegmental nerves. Staining is also apparent at the neuromuscular junction (NMJ). It is concentrated in the synaptic zones at the NMJ.

(Deitcher et al., 1998)

n-syb protein is largely restricted to the presynaptic boutons in larval neuromuscular junctions. Low levels of expression are seen in the motor axons.

(Broadie et al., 1995)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Estes et al., 2000)

colocalizes_with presynaptic active zone

inferred from direct assay

(Petersen and Stowers, 2011)

part_of SNARE complex

inferred from direct assay

(Yu et al., 2011, Wang and Chapman, 2010)

colocalizes_with synaptic vesicle

inferred from direct assay

(Petersen and Stowers, 2011)

located_in synaptic vesicle

inferred from direct assay

(Rolls et al., 2007)

located_in terminal bouton

inferred from direct assay

(Loveall and Deitcher, 2010)

Expression Deduced from Reporters

Reporter: P{GMR57C10-GAL4}

Stage

Tissue/Position (including subcellular localization)

Reference

adult optic lobe neuron | subset

(Wu et al., 2016, Nern et al., 2015)

(Massey et al., 2019)

Reporter: P{GMR57C10-lexA}

Stage

Tissue/Position (including subcellular localization)

Reference

(Vagnoni and Bullock, 2016)

Reporter: P{nSyb-GAL4.P} M{nSyb-GAL4.P}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Bloomington Drosophila Stock Center, 2017, Riabinina and Potter, 2013.10.1)

(Riabinina et al., 2015)

Reporter: P{nSyb-GAL4.S}

Stage

Tissue/Position (including subcellular localization)

Reference

late third instar larval stage

(Yu et al., 2010, Bushey et al., 2009)

Reporter: P{nSyb-GAL4.U}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic/larval neuron | subset of embryonic/larval brain

(Colombani et al., 2015)

Reporter: P{nSyb-lexA.DBD::QF.AD}

Stage

Tissue/Position (including subcellular localization)

Reference

(Riabinina et al., 2015)

Reporter: P{nSyb-QF2.P}

Stage

Tissue/Position (including subcellular localization)

Reference

(Riabinina et al., 2015)

Reporter: P{nSyb-QF2w.P}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

(Riabinina et al., 2015)

Reporter: PBac{nSyb-lexA::p65}

Stage

Tissue/Position (including subcellular localization)

Reference