Gene Dmel\Syx1A

(Schulze, 1994.11.18)

Left of (cM)

Right of (cM)

Notes

Syx1A^3-69 used in recombination mapping.

Gene Model & Products

Please see the GBrowse view of Dmel\Syx1A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.49

Shares 3' UTR sequences with upstream gene.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Syx1A-RA

FBtr0084588

NM_079751

2385

291

Syx1A-RB

FBtr0303161

NM_001202347

2639

296

Additional Transcript Data & Comments

Reported size (kB)

12, 9, 8, 7, 4.4, 3.5 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Syx1A-PA

FBpp0083973

33.6

291

5.09

NP_524475

AAF56232

Syx1A-PB

FBpp0292260

34.2

296

5.20

NP_001189276

ADV37366

Additional Polypeptide Data & Comments

Reported size (kDa)

291 (aa); 35 (kD observed)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Target SNARE coiled-coil domain (IPR000727)

Syntaxin, N-terminal (IPR006011)

Syntaxin/epimorphin, conserved site (IPR006012)

t-SNARE (IPR010989)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q24547

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval peripheral nervous system

posterior midgut primordium

anterior midgut primordium

embryonic/larval neuron

central nervous system

embryonic/larval garland cell

embryonic stage | early

organism | ubiquitous

organism | 0% egg length

Comment:0-1% egg length

embryonic stage 1 -- 3

organism

Comment:maternally deposited

embryonic stage 4 -- 6

organism

embryonic stage 7 -- 8

organism | ubiquitous

embryonic stage 9

anterior midgut primordium

ectoderm

neuroblast

amnioproctodeal invagination

embryonic stage 9 -- 10

organism | ubiquitous

embryonic stage 11 -- 12

organism | ubiquitous

embryonic stage 13 -- 16

embryonic brain

organism | ubiquitous

ventral nerve cord

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

Additional Descriptive Data

Syx1A expression is widespread in the embryo prior to stage 12. After this, expression in ectodermal cells has faded and expression is observed mainly in the anterior and posterior midguts, the garland cells, and the CNS. By stage 14, the major sites of expression are the CNS and the garland cells. Weaker expression is observed in the PNS.

In situ hybridization reveals that Syx1A transcripts are present throuhgout precellularized embryos though they are most prevalent at the posterior pole. After cellularization, Syx1A transcripts are present in all cells and appear to be concentrated near the cell membrane. In stage 9 embryos, transcripts are observed in the anterior and posterior midgut invaginations as well as in the ventral neuroblasts and the ectoderm.

Syx1A transcripts are expressed in a complex pattern throughout development. Up to six transcripts are observed at any given stage on northern blots. In early embryos, the 3.5, 4.2, and 8.0kb transcripts are abundant. In 3-9hr embryos, the 4.2, 8.0, and 9.0kb transcripts predominate. The 7.0 and 12.0kb transcripts appear after 9hr. In first instar larvae, 5 transcripts are prominent. In second and third instar larvae, the number of abundant transcripts is reduced to three and two, respectively. During pupation, 5 transcripts reappear and are also seen in adults.

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

neuron

• axon

bouton

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval garland cell

presumptive embryonic/larval peripheral nervous system

central nervous system

commissure

neuromuscular junction

neuron

• synaptic vesicle

longitudinal connective

neuron

• axon

cell body

embryonic stage | early

organism | ubiquitous

pole bud

embryonic stage 9

ectoderm

mesoderm

ventral neurectoderm

embryonic stage 15 -- 17

anterior embryonic/larval midgut

posterior embryonic/larval midgut

ectoderm

larval stage

neuromuscular junction

synapse

(Schulze and Bellen, 1996, Hiesinger et al., 2001)

third instar larval stage

embryonic/larval neuromuscular junction

• terminal bouton

(Bronk et al., 2001)

adult stage

lamina

medulla

adult brain

photoreceptor cell

• plasma membrane of terminal bouton

(Hiesinger et al., 2001)

adult stage & oogenesis

germarium region 2b

germline cyst

germarium region 3

germarium region 2a

adult stage oogenesis stage S1 -- S8

nurse cell

(Broadie et al., 1995, Schulze et al., 1995)

Additional Descriptive Data

Syx1A protein is enriched in membrane fractions and is associated with synaptic vesicles and possibly other small vesicles such as endosomes.

Syx1A protein is detected in germarium regions 2 and 3 outlining the membranes of the cyst cells. It continues to be abundantly expressed in the nurse cells during oogenesis stages S1-S8. Levels fade during stages S8 and S9. In early embryos, Syx1A protein is present ubiquitously but is concentrated at the posterior tip where it accumulates under the pole buds. At mitotic cycle 13, the protein is concentrated in hexagonal-shaped outlines that correspond to the cleavage furrows of the cytoplasmic buds. After cellularization it is present in all of the membranes of all cells. During germband extension, it is present in the mesoderm, ectoderm, and invaginating neurectoderm but is excluded from the amnioserosa. Syx1A protein is also present in the adult brain and the synaptic substations of the visual system. In the brain it is concentrated in neuropil regions and is somewhat enriched at synaptic regions such as the lamina and the medulla.

syx1a protein is present primarily in the CNS and garland cells though some staining is seen in the ectoderm and the midgut in stages 15-17. In the CNS, staining is most prominent along the longitudinal tracts of the ventral nerve cord and brain but is also found in the commissures of the CNS and the axons of the peripheral nerves. In the periphery of the embryo, staining is seen in neuromuscular junctions and at low levels in some cell bodies.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

References

plasma membrane

inferred from direct assay

(Khanna et al., 2010)

synaptic vesicle

inferred from direct assay

Expression Deduced from Reporters

Reporter: P{PZ}Syx1A⁰⁶⁷³⁷

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

central nervous system

embryonic/larval garland cell

anterior embryonic/larval midgut

posterior embryonic/larval midgut

wandering third instar larval stage

Malpighian tubule

embryonic/larval hindgut

gastric caecum

embryonic/larval midgut | restricted

(The modENCODE Consortium, 2010, Graveley et al., 2011)

adult stage

adult central complex

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		38
Larval Midgut		26.7
Larval Hindgut		32.3
Larval Malpighian Tubules		13.7
Larval Fat Body		9.7
Larval Salivary Gland		26.6
Larval Trachea		53.225
Larval Carcass		21.975
Adult Head		19.3
Adult Eye		36.325
Adult Brain		91.3
Adult Thoracic-Abdominal Ganglion		92.7
Adult Crop		15.4
Adult Midgut		31
Adult Hindgut		29.4
Adult Malpighian Tubules		7.6
Adult Fat Body		8.2
Adult Salivary Gland		29.3
Adult Heart		43.025
Adult VirginFemale Spermatheca		4
Adult InseminatedFemale Spermatheca		13.6
Adult Ovary		16.5
Adult Testis		20.4
Adult Male Accessory Gland		21.3
Adult Carcass		15.8
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		38
Larval Midgut		26.7
Larval Hindgut		32.3
Larval Malpighian Tubules		13.7
Larval Fat Body		9.7
Larval Salivary Gland		26.6
Larval Trachea		53.225
Larval Carcass		21.975
Adult Head		19.3
Adult Eye		36.325
Adult Brain		91.3
Adult Thoracic-Abdominal Ganglion		92.7
Adult Crop		15.4
Adult Midgut		31
Adult Hindgut		29.4
Adult Malpighian Tubules		7.6
Adult Fat Body		8.2
Adult Salivary Gland		29.3
Adult Heart		43.025
Adult VirginFemale Spermatheca		4
Adult InseminatedFemale Spermatheca		13.6
Adult Ovary		16.5
Adult Testis		20.4
Adult Male Accessory Gland		21.3
Adult Carcass		15.8
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		38
Larval Midgut		26.7
Larval Hindgut		32.3
Larval Malpighian Tubules		13.7
Larval Fat Body		9.7
Larval Salivary Gland		26.6
Larval Trachea		53.225
Larval Carcass		21.975
Adult Head		19.3
Adult Eye		36.325
Adult Brain		91.3
Adult Thoracic-Abdominal Ganglion		92.7
Adult Crop		15.4
Adult Midgut		31
Adult Hindgut		29.4
Adult Malpighian Tubules		7.6
Adult Fat Body		8.2
Adult Salivary Gland		29.3
Adult Heart		43.025
Adult VirginFemale Spermatheca		4
Adult InseminatedFemale Spermatheca		13.6
Adult Ovary		16.5
Adult Testis		20.4
Adult Male Accessory Gland		21.3
Adult Carcass		15.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		38
Larval Midgut		26.7
Larval Hindgut		32.3
Larval Malpighian Tubules		13.7
Larval Fat Body		9.7
Larval Salivary Gland		26.6
Larval Trachea		53.225
Larval Carcass		21.975
Adult Head		19.3
Adult Eye		36.325
Adult Brain		91.3
Adult Thoracic-Abdominal Ganglion		92.7
Adult Crop		15.4
Adult Midgut		31
Adult Hindgut		29.4
Adult Malpighian Tubules		7.6
Adult Fat Body		8.2
Adult Salivary Gland		29.3
Adult Heart		43.025
Adult VirginFemale Spermatheca		4
Adult InseminatedFemale Spermatheca		13.6
Adult Ovary		16.5
Adult Testis		20.4
Adult Male Accessory Gland		21.3
Adult Carcass		15.8
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		38
Larval Midgut		26.7
Larval Hindgut		32.3
Larval Malpighian Tubules		13.7
Larval Fat Body		9.7
Larval Salivary Gland		26.6
Larval Trachea		53.225
Larval Carcass		21.975
Adult Head		19.3
Adult Eye		36.325
Adult Brain		91.3
Adult Thoracic-Abdominal Ganglion		92.7
Adult Crop		15.4
Adult Midgut		31
Adult Hindgut		29.4
Adult Malpighian Tubules		7.6
Adult Fat Body		8.2
Adult Salivary Gland		29.3
Adult Heart		43.025
Adult VirginFemale Spermatheca		4
Adult InseminatedFemale Spermatheca		13.6
Adult Ovary		16.5
Adult Testis		20.4
Adult Male Accessory Gland		21.3
Adult Carcass		15.8
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		38
Larval Midgut		26.7
Larval Hindgut		32.3
Larval Malpighian Tubules		13.7
Larval Fat Body		9.7
Larval Salivary Gland		26.6
Larval Trachea		53.225
Larval Carcass		21.975
Adult Head		19.3
Adult Eye		36.325
Adult Brain		91.3
Adult Thoracic-Abdominal Ganglion		92.7
Adult Crop		15.4
Adult Midgut		31
Adult Hindgut		29.4
Adult Malpighian Tubules		7.6
Adult Fat Body		8.2
Adult Salivary Gland		29.3
Adult Heart		43.025
Adult VirginFemale Spermatheca		4
Adult InseminatedFemale Spermatheca		13.6
Adult Ovary		16.5
Adult Testis		20.4
Adult Male Accessory Gland		21.3
Adult Carcass		15.8
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		38
Larval Midgut		26.7
Larval Hindgut		32.3
Larval Malpighian Tubules		13.7
Larval Fat Body		9.7
Larval Salivary Gland		26.6
Larval Trachea		53.225
Larval Carcass		21.975
Adult Head		19.3
Adult Eye		36.325
Adult Brain		91.3
Adult Thoracic-Abdominal Ganglion		92.7
Adult Crop		15.4
Adult Midgut		31
Adult Hindgut		29.4
Adult Malpighian Tubules		7.6
Adult Fat Body		8.2
Adult Salivary Gland		29.3
Adult Heart		43.025
Adult VirginFemale Spermatheca		4
Adult InseminatedFemale Spermatheca		13.6
Adult Ovary		16.5
Adult Testis		20.4
Adult Male Accessory Gland		21.3
Adult Carcass		15.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		38
Larval Midgut		26.7
Larval Hindgut		32.3
Larval Malpighian Tubules		13.7
Larval Fat Body		9.7
Larval Salivary Gland		26.6
Larval Trachea		53.225
Larval Carcass		21.975
Adult Head		19.3
Adult Eye		36.325
Adult Brain		91.3
Adult Thoracic-Abdominal Ganglion		92.7
Adult Crop		15.4
Adult Midgut		31
Adult Hindgut		29.4
Adult Malpighian Tubules		7.6
Adult Fat Body		8.2
Adult Salivary Gland		29.3
Adult Heart		43.025
Adult VirginFemale Spermatheca		4
Adult InseminatedFemale Spermatheca		13.6
Adult Ovary		16.5
Adult Testis		20.4
Adult Male Accessory Gland		21.3
Adult Carcass		15.8
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	no informative data
NA	Eye	36.325
NA	Brain	91.3
38	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	92.7
NA	Crop	no informative data
no informative data	Midgut	no informative data
32.3	Hindgut	29.4
no informative data	Malpighian Tubules	7.6
9.7	Fat Body	8.2
26.6	Salivary Gland	no informative data
NA	Heart	43.025
53.225	Trachea	NA
NA	VirginFemale Spermatheca	4
NA	InseminatedFemale Spermatheca	no informative data
NA	Ovary	no informative data
NA	Testis	20.4
NA	Male Accessory Gland	no informative data
21.975	Carcass	no informative data

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		51
central nervous system, pupae P8		138
head, virgin 1-day female		66
head, virgin 4-day female		46
head, virgin 20-day female		20
head, mated 1-day female		56
head, mated 4-day female		41
head, mated 20-day female		25
head, mated 1-day male		110
head, mated 4-day male		77
head, mated 20-day male		57
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		43
digestive system, 1-day adult		28
digestive system, 4-day adult		27
digestive system, 20-day adult		23
fat body, larvae L3 wandering		8
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		28
carcass, 1-day adult		24
carcass, 4-day adult		19
carcass, 20-day adult		16
ovary, virgin 4-day female		39
ovary, mated 4-day female		47
testis, mated 4-day male		9
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		(51)
central nervous system, pupae P8		(138)
head, virgin 1-day female		(66)
head, virgin 4-day female		(46)
head, virgin 20-day female		20
head, mated 1-day female		(56)
head, mated 4-day female		(41)
head, mated 20-day female		25
head, mated 1-day male		(110)
head, mated 4-day male		(77)
head, mated 20-day male		(57)
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		(43)
digestive system, 1-day adult		(28)
digestive system, 4-day adult		(27)
digestive system, 20-day adult		23
fat body, larvae L3 wandering		8
fat body, white prepupae		11
fat body, pupae P8		(42)
carcass, larvae L3 wandering		(28)
carcass, 1-day adult		24
carcass, 4-day adult		19
carcass, 20-day adult		16
ovary, virgin 4-day female		(39)
ovary, mated 4-day female		(47)
testis, mated 4-day male		9
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		51
central nervous system, pupae P8		(138)
head, virgin 1-day female		66
head, virgin 4-day female		46
head, virgin 20-day female		20
head, mated 1-day female		56
head, mated 4-day female		41
head, mated 20-day female		25
head, mated 1-day male		(110)
head, mated 4-day male		77
head, mated 20-day male		57
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		43
digestive system, 1-day adult		28
digestive system, 4-day adult		27
digestive system, 20-day adult		23
fat body, larvae L3 wandering		8
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		28
carcass, 1-day adult		24
carcass, 4-day adult		19
carcass, 20-day adult		16
ovary, virgin 4-day female		39
ovary, mated 4-day female		47
testis, mated 4-day male		9
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		51
central nervous system, pupae P8		138
head, virgin 1-day female		66
head, virgin 4-day female		46
head, virgin 20-day female		20
head, mated 1-day female		56
head, mated 4-day female		41
head, mated 20-day female		25
head, mated 1-day male		110
head, mated 4-day male		77
head, mated 20-day male		57
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		43
digestive system, 1-day adult		28
digestive system, 4-day adult		27
digestive system, 20-day adult		23
fat body, larvae L3 wandering		8
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		28
carcass, 1-day adult		24
carcass, 4-day adult		19
carcass, 20-day adult		16
ovary, virgin 4-day female		39
ovary, mated 4-day female		47
testis, mated 4-day male		9
accessory gland, mated 4-day male		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		51
central nervous system, pupae P8		138
head, virgin 1-day female		66
head, virgin 4-day female		46
head, virgin 20-day female		20
head, mated 1-day female		56
head, mated 4-day female		41
head, mated 20-day female		25
head, mated 1-day male		110
head, mated 4-day male		77
head, mated 20-day male		57
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		43
digestive system, 1-day adult		28
digestive system, 4-day adult		27
digestive system, 20-day adult		23
fat body, larvae L3 wandering		8
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		28
carcass, 1-day adult		24
carcass, 4-day adult		19
carcass, 20-day adult		16
ovary, virgin 4-day female		39
ovary, mated 4-day female		47
testis, mated 4-day male		9
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		(51)
central nervous system, pupae P8		(138)
head, virgin 1-day female		(66)
head, virgin 4-day female		(46)
head, virgin 20-day female		20
head, mated 1-day female		(56)
head, mated 4-day female		(41)
head, mated 20-day female		25
head, mated 1-day male		(110)
head, mated 4-day male		(77)
head, mated 20-day male		(57)
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		(43)
digestive system, 1-day adult		28
digestive system, 4-day adult		27
digestive system, 20-day adult		23
fat body, larvae L3 wandering		8
fat body, white prepupae		11
fat body, pupae P8		(42)
carcass, larvae L3 wandering		28
carcass, 1-day adult		24
carcass, 4-day adult		19
carcass, 20-day adult		16
ovary, virgin 4-day female		(39)
ovary, mated 4-day female		(47)
testis, mated 4-day male		9
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		51
central nervous system, pupae P8		138
head, virgin 1-day female		66
head, virgin 4-day female		46
head, virgin 20-day female		20
head, mated 1-day female		56
head, mated 4-day female		41
head, mated 20-day female		25
head, mated 1-day male		110
head, mated 4-day male		77
head, mated 20-day male		57
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		43
digestive system, 1-day adult		28
digestive system, 4-day adult		27
digestive system, 20-day adult		23
fat body, larvae L3 wandering		8
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		28
carcass, 1-day adult		24
carcass, 4-day adult		19
carcass, 20-day adult		16
ovary, virgin 4-day female		39
ovary, mated 4-day female		47
testis, mated 4-day male		9
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		26
central nervous system, larvae L3		51
central nervous system, pupae P8		138
head, virgin 1-day female		66
head, virgin 4-day female		46
head, virgin 20-day female		20
head, mated 1-day female		56
head, mated 4-day female		41
head, mated 20-day female		25
head, mated 1-day male		110
head, mated 4-day male		77
head, mated 20-day male		57
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		43
digestive system, 1-day adult		28
digestive system, 4-day adult		27
digestive system, 20-day adult		23
fat body, larvae L3 wandering		8
fat body, white prepupae		11
fat body, pupae P8		42
carcass, larvae L3 wandering		28
carcass, 1-day adult		24
carcass, 4-day adult		19
carcass, 20-day adult		16
ovary, virgin 4-day female		39
ovary, mated 4-day female		47
testis, mated 4-day male		9
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during early larval stages, during late pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		15
embryo 02-04hr		28
embryo 04-06hr		34
embryo 06-08hr		18
embryo 08-10hr		18
embryo 10-12hr		13
embryo 12-14hr		17
embryo 14-16hr		21
embryo 16-18hr		30
embryo 18-20hr		31
embryo 20-22hr		42
embryo 22-24hr		43
larva L1		29
larva L2		20
larva L3 12hr old		14
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		12
white prepupae 24hr		15
pupae 2d postWPP		20
pupae 3d postWPP		23
pupae 4d postWPP		17
adult male 01day		18
adult male 05day		16
adult male 30day		13
adult female 01day		14
adult female 05day		13
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		15
embryo 02-04hr		(28)
embryo 04-06hr		(34)
embryo 06-08hr		18
embryo 08-10hr		18
embryo 10-12hr		13
embryo 12-14hr		17
embryo 14-16hr		21
embryo 16-18hr		(30)
embryo 18-20hr		(31)
embryo 20-22hr		(42)
embryo 22-24hr		(43)
larva L1		(29)
larva L2		20
larva L3 12hr old		14
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		12
white prepupae 24hr		15
pupae 2d postWPP		20
pupae 3d postWPP		23
pupae 4d postWPP		17
adult male 01day		18
adult male 05day		16
adult male 30day		13
adult female 01day		14
adult female 05day		13
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		15
embryo 02-04hr		28
embryo 04-06hr		34
embryo 06-08hr		18
embryo 08-10hr		18
embryo 10-12hr		13
embryo 12-14hr		17
embryo 14-16hr		21
embryo 16-18hr		30
embryo 18-20hr		31
embryo 20-22hr		42
embryo 22-24hr		43
larva L1		29
larva L2		20
larva L3 12hr old		14
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		12
white prepupae 24hr		15
pupae 2d postWPP		20
pupae 3d postWPP		23
pupae 4d postWPP		17
adult male 01day		18
adult male 05day		16
adult male 30day		13
adult female 01day		14
adult female 05day		13
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		15
embryo 02-04hr		28
embryo 04-06hr		34
embryo 06-08hr		18
embryo 08-10hr		18
embryo 10-12hr		13
embryo 12-14hr		17
embryo 14-16hr		21
embryo 16-18hr		30
embryo 18-20hr		31
embryo 20-22hr		42
embryo 22-24hr		43
larva L1		29
larva L2		20
larva L3 12hr old		14
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		12
white prepupae 24hr		15
pupae 2d postWPP		20
pupae 3d postWPP		23
pupae 4d postWPP		17
adult male 01day		18
adult male 05day		16
adult male 30day		13
adult female 01day		14
adult female 05day		13
adult female 30day		13
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		15
embryo 02-04hr		28
embryo 04-06hr		34
embryo 06-08hr		18
embryo 08-10hr		18
embryo 10-12hr		13
embryo 12-14hr		17
embryo 14-16hr		21
embryo 16-18hr		30
embryo 18-20hr		31
embryo 20-22hr		42
embryo 22-24hr		43
larva L1		29
larva L2		20
larva L3 12hr old		14
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		12
white prepupae 24hr		15
pupae 2d postWPP		20
pupae 3d postWPP		23
pupae 4d postWPP		17
adult male 01day		18
adult male 05day		16
adult male 30day		13
adult female 01day		14
adult female 05day		13
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		15
embryo 02-04hr		28
embryo 04-06hr		(34)
embryo 06-08hr		18
embryo 08-10hr		18
embryo 10-12hr		13
embryo 12-14hr		17
embryo 14-16hr		21
embryo 16-18hr		30
embryo 18-20hr		31
embryo 20-22hr		(42)
embryo 22-24hr		(43)
larva L1		29
larva L2		20
larva L3 12hr old		14
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		12
white prepupae 24hr		15
pupae 2d postWPP		20
pupae 3d postWPP		23
pupae 4d postWPP		17
adult male 01day		18
adult male 05day		16
adult male 30day		13
adult female 01day		14
adult female 05day		13
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		15
embryo 02-04hr		28
embryo 04-06hr		34
embryo 06-08hr		18
embryo 08-10hr		18
embryo 10-12hr		13
embryo 12-14hr		17
embryo 14-16hr		21
embryo 16-18hr		30
embryo 18-20hr		31
embryo 20-22hr		42
embryo 22-24hr		43
larva L1		29
larva L2		20
larva L3 12hr old		14
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		12
white prepupae 24hr		15
pupae 2d postWPP		20
pupae 3d postWPP		23
pupae 4d postWPP		17
adult male 01day		18
adult male 05day		16
adult male 30day		13
adult female 01day		14
adult female 05day		13
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		15
embryo 02-04hr		28
embryo 04-06hr		34
embryo 06-08hr		18
embryo 08-10hr		18
embryo 10-12hr		13
embryo 12-14hr		17
embryo 14-16hr		21
embryo 16-18hr		30
embryo 18-20hr		31
embryo 20-22hr		42
embryo 22-24hr		43
larva L1		29
larva L2		20
larva L3 12hr old		14
larva L3 puffstage 1-2		6
larva L3 puffstage 3-6		14
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		12
white prepupae 24hr		15
pupae 2d postWPP		20
pupae 3d postWPP		23
pupae 4d postWPP		17
adult male 01day		18
adult male 05day		16
adult male 30day		13
adult female 01day		14
adult female 05day		13
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		87
Schneider line 2 Sg4		24
embryonic 1182-4H		92
embryonic GM2		57
embryonic Kc167		65
embryonic S1		40
embryonic S3		111
leg disc CME L1		41
wing disc CME-W2		42
wing disc ML-DmD8		38
wing disc ML-DmD9		50
wing disc ML-DmD16-c3		48
wing disc ML-DmD21		8
wing disc ML-DmD32		70
haltere disc ML-DmD17-c3		33
eye-antennal disc ML-DmD11		43
antennal disc ML-DmD20-c5		50
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		52
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		65
ovary fGS/OSS		41
ovary OSC		64
ovary OSS		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(87)
Schneider line 2 Sg4		24
embryonic 1182-4H		(92)
embryonic GM2		(57)
embryonic Kc167		(65)
embryonic S1		(40)
embryonic S3		(111)
leg disc CME L1		(41)
wing disc CME-W2		(42)
wing disc ML-DmD8		(38)
wing disc ML-DmD9		(50)
wing disc ML-DmD16-c3		(48)
wing disc ML-DmD21		8
wing disc ML-DmD32		(70)
haltere disc ML-DmD17-c3		(33)
eye-antennal disc ML-DmD11		(43)
antennal disc ML-DmD20-c5		(50)
mixed discs ML-DmD4-c1		(28)
CNS ML-DmBG1-c1		(52)
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		(65)
ovary fGS/OSS		(41)
ovary OSC		(64)
ovary OSS		26
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		87
Schneider line 2 Sg4		24
embryonic 1182-4H		92
embryonic GM2		57
embryonic Kc167		65
embryonic S1		40
embryonic S3		(111)
leg disc CME L1		41
wing disc CME-W2		42
wing disc ML-DmD8		38
wing disc ML-DmD9		50
wing disc ML-DmD16-c3		48
wing disc ML-DmD21		8
wing disc ML-DmD32		70
haltere disc ML-DmD17-c3		33
eye-antennal disc ML-DmD11		43
antennal disc ML-DmD20-c5		50
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		52
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		65
ovary fGS/OSS		41
ovary OSC		64
ovary OSS		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		87
Schneider line 2 Sg4		24
embryonic 1182-4H		92
embryonic GM2		57
embryonic Kc167		65
embryonic S1		40
embryonic S3		111
leg disc CME L1		41
wing disc CME-W2		42
wing disc ML-DmD8		38
wing disc ML-DmD9		50
wing disc ML-DmD16-c3		48
wing disc ML-DmD21		8
wing disc ML-DmD32		70
haltere disc ML-DmD17-c3		33
eye-antennal disc ML-DmD11		43
antennal disc ML-DmD20-c5		50
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		52
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		65
ovary fGS/OSS		41
ovary OSC		64
ovary OSS		26
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		87
Schneider line 2 Sg4		24
embryonic 1182-4H		92
embryonic GM2		57
embryonic Kc167		65
embryonic S1		40
embryonic S3		111
leg disc CME L1		41
wing disc CME-W2		42
wing disc ML-DmD8		38
wing disc ML-DmD9		50
wing disc ML-DmD16-c3		48
wing disc ML-DmD21		8
wing disc ML-DmD32		70
haltere disc ML-DmD17-c3		33
eye-antennal disc ML-DmD11		43
antennal disc ML-DmD20-c5		50
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		52
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		65
ovary fGS/OSS		41
ovary OSC		64
ovary OSS		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(87)
Schneider line 2 Sg4		24
embryonic 1182-4H		(92)
embryonic GM2		(57)
embryonic Kc167		(65)
embryonic S1		(40)
embryonic S3		(111)
leg disc CME L1		(41)
wing disc CME-W2		(42)
wing disc ML-DmD8		(38)
wing disc ML-DmD9		(50)
wing disc ML-DmD16-c3		(48)
wing disc ML-DmD21		8
wing disc ML-DmD32		(70)
haltere disc ML-DmD17-c3		(33)
eye-antennal disc ML-DmD11		(43)
antennal disc ML-DmD20-c5		(50)
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		(52)
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		(65)
ovary fGS/OSS		(41)
ovary OSC		(64)
ovary OSS		26
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		87
Schneider line 2 Sg4		24
embryonic 1182-4H		92
embryonic GM2		57
embryonic Kc167		65
embryonic S1		40
embryonic S3		111
leg disc CME L1		41
wing disc CME-W2		42
wing disc ML-DmD8		38
wing disc ML-DmD9		50
wing disc ML-DmD16-c3		48
wing disc ML-DmD21		8
wing disc ML-DmD32		70
haltere disc ML-DmD17-c3		33
eye-antennal disc ML-DmD11		43
antennal disc ML-DmD20-c5		50
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		52
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		65
ovary fGS/OSS		41
ovary OSC		64
ovary OSS		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		87
Schneider line 2 Sg4		24
embryonic 1182-4H		92
embryonic GM2		57
embryonic Kc167		65
embryonic S1		40
embryonic S3		111
leg disc CME L1		41
wing disc CME-W2		42
wing disc ML-DmD8		38
wing disc ML-DmD9		50
wing disc ML-DmD16-c3		48
wing disc ML-DmD21		8
wing disc ML-DmD32		70
haltere disc ML-DmD17-c3		33
eye-antennal disc ML-DmD11		43
antennal disc ML-DmD20-c5		50
mixed discs ML-DmD4-c1		28
CNS ML-DmBG1-c1		52
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		65
ovary fGS/OSS		41
ovary OSC		64
ovary OSS		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		35
cold shock, 4-day adult		31
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		15
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		54
Copper 0.5 mM 12 hrs, larvae L3		33
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		32
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		19
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		14
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(35)
cold shock, 4-day adult		(31)
heat shock, 4-day adult		(29)
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		15
Cadmium 50 mM 48 hrs, 4-day adult		(27)
Cadmium 100 mM 48 hrs, 4-day adult		(54)
Copper 0.5 mM 12 hrs, larvae L3		(33)
Copper 15 mM 48 hrs, 4-day adult		(44)
Zinc 5 mM 12 hrs, larvae L3		(27)
Zinc 4.5 mM 48 hrs, 4-day adult		(32)
Ethanol 2.5% 3 hrs, larvae L3		(34)
Ethanol 5% 3 hrs, larvae L3		19
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(37)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(31)
Paraquat 5 mM 48 hrs, 4-day adult		(34)
Paraquat 10 mM 48 hrs, 4-day adult		(32)
Rotenone 2 μg 12 hrs, larvae L3		14
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		35
cold shock, 4-day adult		31
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		15
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		54
Copper 0.5 mM 12 hrs, larvae L3		33
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		32
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		19
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		14
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		35
cold shock, 4-day adult		31
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		15
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		54
Copper 0.5 mM 12 hrs, larvae L3		33
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		32
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		19
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		14
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		35
cold shock, 4-day adult		31
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		15
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		54
Copper 0.5 mM 12 hrs, larvae L3		33
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		32
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		19
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		14
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(35)
cold shock, 4-day adult		31
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		15
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		(54)
Copper 0.5 mM 12 hrs, larvae L3		(33)
Copper 15 mM 48 hrs, 4-day adult		(44)
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		32
Ethanol 2.5% 3 hrs, larvae L3		(34)
Ethanol 5% 3 hrs, larvae L3		19
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(37)
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		(34)
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		14
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		35
cold shock, 4-day adult		31
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		15
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		54
Copper 0.5 mM 12 hrs, larvae L3		33
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		32
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		19
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		14
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		35
cold shock, 4-day adult		31
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		17
Cadmium 50 mM 12 hrs, larvae L3		15
Cadmium 50 mM 48 hrs, 4-day adult		27
Cadmium 100 mM 48 hrs, 4-day adult		54
Copper 0.5 mM 12 hrs, larvae L3		33
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		32
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		19
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		15
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		37
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		14
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

neuroanatomy defective | embryonic stage

BDGP expression data

•

LD43943

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0013343

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG33100-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

Syx1A^GD564

lethal | embryonic stage

Syx1A^Δ229

lethal | embryonic stage, with Syx1A^A243V.V247A

Syx1A^Δ229

lethal | embryonic stage, with Syx1A^Δ229

Syx1A^A243V.V247A

lethal | embryonic stage | maternal effect

Syx1A^Δ229

lethal | embryonic stage | recessive

lethal | embryonic stage | recessive | rescuable maternal effect

Syx1A^L266

lethal | embryonic stage | rescuable maternal effect

Syx1A⁰⁶⁷³⁷

lethal | partially

lethal | recessive

viable

viable, with Syx1A^+t13.5

Syx1A^Δ229

viable, with Syx1A^Δ229

Syx1A^+t13.5

Other Phenotypes

Allele

cytokinesis defective

Syx1A^{dsRNA.358-1114}

hypoactive, with Syx1A^A243V.V247A

Syx1A^Δ229

hypoactive, with Syx1A^Δ229

Syx1A^A243V.V247A

neuroanatomy defective

Syx1A^3-69

Syx1A^Δ229

neuroanatomy defective | embryonic stage | recessive

Syx1A^Δ229

neurophysiology defective

neurophysiology defective, with Syx1A^A243V.V247A

Syx1A^Δ229

neurophysiology defective, with Syx1A^Δ229

Syx1A^A243V.V247A

neurophysiology defective | embryonic stage | recessive

Syx1A^Δ229

neurophysiology defective | heat sensitive

Syx1A^3-69

neurophysiology defective | heat sensitive, with Scer\GAL4^elav-C155, Syx1A^3-69, Syx1A^Scer\UAS.cBa

Syx1A^Δ229

neurophysiology defective | heat sensitive, with Scer\GAL4^elav-C155, Syx1A^Δ229, Syx1A^3-69

Syx1A^Scer\UAS.cBa

neurophysiology defective | heat sensitive, with Scer\GAL4^elav-C155, Syx1A^Δ229, Syx1A^Scer\UAS.cBa

Syx1A^3-69

neurophysiology defective | heat sensitive, with Scer\GAL4^elav-C155, Syx1A^Δ229/Syx1A^3-69

Syx1A^Scer\UAS.cBa

neurophysiology defective | heat sensitive (with Syx1A^3-69)

Syx1A^Δ229

neurophysiology defective | heat sensitive (with Syx1A^3-69), with Scer\GAL4^elav-C155, Syx1A^Scer\UAS.cBa

Syx1A^Δ229

neurophysiology defective | heat sensitive (with Syx1A^Δ229)

Syx1A^3-69

neurophysiology defective | heat sensitive (with Syx1A^Δ229), with Scer\GAL4^elav-C155, Syx1A^Scer\UAS.cBa

Syx1A^3-69

neurophysiology defective | larval stage (with Syx1A^L2)

Syx1A^L247

neurophysiology defective | larval stage (with Syx1A^L247)

Syx1A^L2

neurophysiology defective | larval stage | heat sensitive

Syx1A^hs.PW

paralytic | heat sensitive

Syx1A^3-69

paralytic | semidominant | heat sensitive

Syx1A^3-69

uncoordinated | dominant | heat sensitive

Syx1A^3-69

visible | recessive | somatic clone

Syx1A^L371

visual behavior defective | recessive | heat sensitive

Syx1A^3-69

wild-type

Phenotype manifest in

Allele

actomyosin contractile ring

Syx1A^{dsRNA.358-1114}

cytoskeleton | embryonic stage | germline clone

Syx1A^L266

denticle

denticle belt

Syx1A^Δ229

egg | germline clone

Syx1A^L266

embryo | germline clone

Syx1A^L266

embryonic/first instar larval cuticle

embryonic/first instar larval cuticle | maternal effect

Syx1A^Δ229

embryonic/larval midgut | embryonic stage | maternal effect

Syx1A^Δ229

embryonic/larval midgut primordium

Syx1A^Δ229

embryonic/larval muscle system

embryonic epidermis

embryonic foregut

Syx1A^Δ229

embryonic hindgut

Syx1A^Δ229

eye, with Scer\GAL4^GMR.PF

Syx1A^Scer\UAS.cBa

eye photoreceptor cell | somatic clone

Syx1A^L371

intersegmental nerve | embryonic stage

Syx1A^Δ6

intersegmental nerve | embryonic stage | maternal effect

Syx1A^Δ229

longitudinal connective

Syx1A^Δ6

longitudinal connective | maternal effect

Syx1A^Δ229

mitotic telophase

Syx1A^{dsRNA.358-1114}

mitotic telophase & spindle

Syx1A^{dsRNA.358-1114}

mouth hooks

Syx1A^Δ229

neuromuscular junction

neuromuscular junction | embryonic stage

neuromuscular junction | larval stage

Syx1A^hs.PW

nucleus | embryonic stage | germline clone

Syx1A^L266

ommatidium

ommatidium, with Scer\GAL4^GMR.PF

Syx1A^Scer\UAS.cBa

ommatidium | maternal effect

Syx1A^Δ229

oocyte

Syx1A^Δ229

oocyte | germline clone

Syx1A^L371

optic cartridge & synaptic vesicle

Syx1A^3-69

retina

Syx1A^3-69

segmental nerve | embryonic stage

Syx1A^Δ6

segmental nerve | embryonic stage | maternal effect

Syx1A^Δ229

somatic muscle

Syx1A⁰⁶⁷³⁷

synapse (with Syx1A^L2)

Syx1A^L247

synapse (with Syx1A^L247)

Syx1A^L2

synapse | embryonic stage, with Syx1A^A243V.V247A

Syx1A^Δ229

synapse | embryonic stage, with Syx1A^Δ229

Syx1A^A243V.V247A

ventral nerve cord

Syx1A^Δ229

ventral nerve cord | maternal effect

Syx1A^Δ229

wing

wing | maternal effect

Syx1A^Δ229

Classical Alleles ( 29 )

For All Classical Alleles Show

Allele of Syx1A	Class	Mutagen	Stocks	Known lesion
Syx1A^Δ229	amorphic allele - genetic evidence, loss of function allele	Delta2-3	2	Yes
Syx1A⁰⁶⁷³⁷	hypomorphic allele - genetic evidence	P-element activity	1	Yes
Syx1A⁰⁵⁶¹⁸		P-element activity	0	--
Syx1A¹⁰⁶⁶⁰		P-element activity	0	Yes
Syx1A¹¹⁸		Delta2-3	0	Yes
Syx1A¹⁴	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
Syx1A^15ts		ethyl methanesulfonate	0	--
Syx1A¹⁷⁷	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
Syx1A¹⁷		Delta2-3	0	Yes
Syx1A¹	loss of function allele	ethyl methanesulfonate	0	Yes
Syx1A²⁰⁴	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
Syx1A²⁸⁴	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
Syx1A²⁸⁷	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
Syx1A²⁹⁴		Delta2-3	0	Yes
Syx1A²		Delta2-3	0	Yes
Syx1A^3-69		ethyl methanesulfonate	0	Yes
Syx1A⁴	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
Syx1A⁸⁶	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
Syx1A^c05841		piggyBac transposase	0	--
Syx1A^DG10811			0	--
Syx1A^EP28		P-element activity	0	Yes
Syx1A^EP3215		P-element activity	0	Yes
Syx1A^j4D9		P-element activity	0	--
Syx1A^j5E6			0	--
Syx1A^L247		P-element activity	0	--
Syx1A^L266	hypomorphic allele - genetic evidence	P-element activity	0	Yes
Syx1A^L2		P-element activity	0	--
Syx1A^L371	loss of function allele	P-element activity	0	Yes
Syx1A^Δ6	hypomorphic allele - genetic evidence	Delta2-3	0	Yes

Alleles Carried on Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Syx1A	Class	Mutagen	Stocks	Known lesion
Syx1A^GD564		in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Syx1A^JF01829		in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Syx1A^+t11		in vitro construct - genomic fragment	0	Yes
Syx1A^+t13.5		in vitro construct - genomic fragment	0	Yes
Syx1A^+t13		in vitro construct - genomic fragment	0	Yes
Syx1A^+t6		in vitro construct - genomic fragment	0	Yes
Syx1A^A243V.V247A	loss of function allele	in vitro construct - amino acid replacement	0	Yes
Syx1A^DN.Scer\UAS		in vitro construct - regulatory fusion	0	Yes
Syx1A^{dsRNA.358-1114}		in vitro construct - RNAi	0	Yes
Syx1A^H3-C		in vitro construct - deletion	0	Yes
Syx1A^H3-N		in vitro construct - deletion	0	Yes
Syx1A^hs.PW		in vitro construct - regulatory fusion	0	Yes
Syx1A^I212A		in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis	0	Yes
Syx1A^I236A		in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis	0	Yes
Syx1A^NIG.5448R		in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Syx1A^Scer\UAS.cBa		in vitro construct - regulatory fusion	0	Yes
Syx1A^{T254I.Scer\UAS}		in vitro construct - regulatory fusion in vitro construct - amino acid replacement	0	Yes
Syx1A^{tCH322-142K16}		in vitro construct - genomic fragment	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3L)ri-XT1

(BDGP Project Members, 1994-1999)

Df(3R)crb87-4

(Littleton et al., 1998, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(3R)crb-F89-4

(Schulze et al., 1995, Schulze and Bellen, 1996)

Df(3R)Exel6197

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

heat-shock construct

characterization construct

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}Syx1A^EP28

P{EP}Syx1A^EP3215

P{GSV1}C790^C790

insertion of enhancer trap

P{lacW}Syx1A^j4D9

P{lacZ}Syx1A^L2

P{lacZ}Syx1A^L247

P{PZ}Syx1A⁰⁵⁶¹⁸

P{PZ}Syx1A⁰⁶⁷³⁷

P{PZ}Syx1A¹⁰⁶⁶⁰

miscellaneous insertions

P{5'3'}Syx1A²⁰⁴

P{UASp-YFP.Rab3}4EHP^05b

P{wHy}4EHP^DG10811

PBac{PB}Syx1A^c05841

Gene Ontology: Function, Process & Cellular Component ( 18 unique terms )

Terms Based on Experimental Evidence ( 7 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

(Somma et al., 2002, Echard et al., 2004)

References

cytokinesis

inferred from mutant phenotype

haltere development

inferred from genetic interaction with Ubx

(Pallavi et al., 2006)

neurotransmitter secretion

inferred from mutant phenotype

regulation of pole plasm oskar mRNA localization

inferred from mutant phenotype

(Ruden et al., 2000)

synaptic vesicle fusion to presynaptic membrane

inferred from mutant phenotype

(Lagow et al., 2007)

Cellular Component

CV term

References

plasma membrane

inferred from direct assay

(Khanna et al., 2010)

synaptic vesicle

inferred from direct assay

Terms Based on Predictions or Assertions ( 13 terms )

Molecular Function

CV term

References

SNAP receptor activity

inferred from sequence or structural similarity

(FlyBase Genome Annotators, 2002-2003)

inferred from sequence or structural similarity with UniProtKB:O35526

non-traceable author statement

(Schulze, 1994.11.18, Littleton et al., 1999)

Biological Process

CV term

(Mazumdar and Mazumdar, 2002)

References

cellularization

non-traceable author statement

(Tepass et al., 2001)

traceable author statement

cuticle development

non-traceable author statement

(Swiss-Prot Project Members, 1997.2.1)

exocytosis

non-traceable author statement

(Broadie, 1995)

intracellular protein transport

inferred from electronic annotation with InterPro:IPR006012

(FlyBase Curators et al., 2004-)

muscle contraction

non-traceable author statement

(Swiss-Prot Project Members, 1997.2.1)

neurotransmitter secretion

non-traceable author statement

(Lloyd et al., 2000, Swiss-Prot Project Members, 1997.2.1)

synaptic transmission

non-traceable author statement

(Broadie, 1995)

synaptic vesicle docking involved in exocytosis

inferred from sequence or structural similarity

(FlyBase Genome Annotators, 2002-2003)

inferred from sequence or structural similarity with UniProtKB:O35526

vesicle-mediated transport

non-traceable author statement

(Lloyd et al., 2000)

Cellular Component

CV term

(FlyBase Genome Annotators, 2002-2003)

References

integral to membrane

inferred from sequence or structural similarity with UniProtKB:O35526

membrane

non-traceable author statement

(Swiss-Prot Project Members, 1997.2.1)

plasma membrane

inferred from sequence or structural similarity

(Schulze, 1994.11.18, Littleton et al., 1999)

non-traceable author statement

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

Syx1A - Arpc4

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Arpc5

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - AttD

experimental knowledge based

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - Bet1

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

Syx1A - CG15098

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - CG15784

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - CG1599

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

Syx1A - CG2023

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - CG2064

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - CG2065

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - CG3164

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - CG32138

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - CG42668

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Syx1A - CG6208

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - CG9318

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Gos28

experimental knowledge based

Syx1A - Hel25E

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Karyβ3

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - Nsf2

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

Syx1A - PMCA

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Past1

experimental knowledge based

Syx1A - Pvr

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Rab2

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Rab6

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Rme-8

experimental knowledge based

Syx1A - RpLP0

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Rs1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - Sec22

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

Syx1A - Slh

experimental knowledge based

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Syx1A - Snap

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Snap24

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - Snap25

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Syx1A - Syb

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - Syx13

experimental knowledge based

Syx1A - Syx16

experimental knowledge based

Syx1A - Syx18

experimental knowledge based

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Syx1A - Syx5

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Syx7

experimental knowledge based

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Syx1A - Syx8

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - TFAM

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Use1

experimental knowledge based

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Syx1A - Vti1

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - Ykt6

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - ade5

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - eIF3-S8

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - jagn

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Syx1A - koko

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - l(1)G0230

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - membrin

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

Syx1A - n-syb

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Syx1A - porin

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - sun

anti tag coimmunoprecipitation, peptide massfingerprinting

Syx1A - tomosyn

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Syx1A - usnp

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

Syx1A - γSnap

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Syx1A allele

Gene

References

Syx1A^DN.Scer\UAS

coro

(Bharathi et al., 2004)

(Stewart et al., 2001)

Syx1A^Δ229

smi26D

(Anholt et al., 2003)

unspecified

bchs

(Simonsen et al., 2007)

Snap

(Babcock et al., 2004)

Ubx

(Pallavi et al., 2006)

External Data

y¹ v¹; P{TRiP.JF01829}attP2

BioGRID - A database of protein and genetic interactions

•

67788

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0013343

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0013343

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

42854

Orthologs

OrthoDB Orthologs (61) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6GB7SB

Dipteran inclusive ortholog search

EOG6JDHGP

Insect inclusive ortholog search

EOG6C2G6D

Arthropod inclusive ortholog search

EOG6Q2BXD

Metazoa inclusive ortholog search

EOG62RS2N

Orthologs in Drosophila Species (EOG6GB7SB)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Syx1A

Drosophila ananassae

Dana\GF23231

Drosophila pseudoobscura pseudoobscura

Dpse\GA16036

Drosophila persimilis

Dper\GL24148

Drosophila mojavensis

Dmoj\GI23434

Orthologs in non-Drosophila Dipterans (EOG6JDHGP)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009304

Anopheles gambiae

Malaria mosquito

Agam\AGAP007698

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ000184

Orthologs in non-Dipteran Insects (EOG6C2G6D)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB43089

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG009300

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH11437

Atta cephalotes

Leafcutter ant

Acep\ACEP21777

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO20926

Linepithema humile

Argentine ant

Lhum\LH26154

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB27365

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB10256

Solenopsis invicta

Red fire ant

Sinv\SINV12509

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005967

Bombyx mori

Silkmoth

Bmor\BGIBMGA006590

Pediculus humanus

Human body louse

Phum\PHUM095410

Tribolium castaneum

Red flour beetle

Tcas\Syx1A

Orthologs in non-Insect Arthropods (EOG6Q2BXD)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_442635

Ixodes scapularis

Deer tick

Isca\ISCW023653

Orthologs in non-Arthropod Metazoa (EOG62RS2N)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\F56A8.7

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_012329gn

Danio rerio

Zebrafish

Drer\ENSDARG00000004618

Danio rerio

Zebrafish

Drer\si:dkey-245g2.3

Danio rerio

Zebrafish

Drer\stx11b.2

Danio rerio

Zebrafish

Drer\stx1b

Danio rerio

Zebrafish

Drer\ENSDARG00000058994

Danio rerio

Zebrafish

Drer\zgc:165520

Danio rerio

Zebrafish

Drer\stx3a

Danio rerio

Zebrafish

Drer\stx11b.1

Danio rerio

Zebrafish

Drer\stx11a

Danio rerio

Zebrafish

Drer\stx4

Xenopus tropicalis

Western clawed frog

Xtro\stx3

Xenopus tropicalis

Western clawed frog

Xtro\stx11

Xenopus tropicalis

Western clawed frog

Xtro\stx1a

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000001912

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000030140

Xenopus tropicalis

Western clawed frog

Xtro\stx2

Gallus gallus

Domestic chicken

Ggal\STX2

Gallus gallus

Domestic chicken

Ggal\STX1A

Gallus gallus

Domestic chicken

Ggal\STX11

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000020514

Mus musculus

House mouse

Mmus\Stx1a

Mus musculus

House mouse

Mmus\Stx3

Mus musculus

House mouse

Mmus\Stx1b

Mus musculus

House mouse

Mmus\Stx4a

Mus musculus

House mouse

Mmus\Stx11

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000000936

Rattus norvegicus

Norway rat

Rnor\Stx3

Rattus norvegicus

Norway rat

Rnor\Stx1a

Rattus norvegicus

Norway rat

Rnor\Stx4

Rattus norvegicus

Norway rat

Rnor\Stx1b

Homo sapiens

Human

Hsap\STX3A

Homo sapiens

Human

Hsap\ENSG00000111450

Homo sapiens

Human

Hsap\STX1A

Homo sapiens

Human

Hsap\STX11

Homo sapiens

Human

Hsap\STX4

Homo sapiens

Human

Hsap\STX1B

Human Orthologs (6)

Gene

OMIM

HGNC

AAA Orthologs (4) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila ananassae

Dana\GF23231

Drosophila pseudoobscura pseudoobscura

Dpse\GA16036

Drosophila persimilis

Dper\GL24148

Drosophila mojavensis

Dmoj\GI23434

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

Bloomington

25811

4379

Syx1A^Δ229 ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

11697

ry⁵⁰⁶ P{PZ}Syx1A⁰⁶⁷³⁷ 4EHP⁰⁶⁷³⁷/TM3, ry^RK Sb¹ Ser¹

Kyoto

107713

Syx1A^Δ229 ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

VDRC

v33112

w¹¹¹⁸; P{GD564}v33112

v33113

w¹¹¹⁸; P{GD564}v33113/TM3

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 77 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD43943

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0013343

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

42854

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: syx1A CG5448

(Kodira, 1999.11)

Source for database merge of

Source for merge of: Syx1A anon-EST:Gibbs4

Source for merge of: Syx1A anon-WO02059370.54

Source for merge of: Syx1A CG10716

Source for merge of: syx1A CG18615

(FlyBase Genome Annotators, 2002-2003, FlyBase, 1996-)

Additional comments

Source for merge of Syx1A anon-EST:Gibbs4 was sequence comparison (date:030707).

Source for merge of syx1A CG18615 was sequence comparison (date:001104).

Source for merge of Syx1A anon-WO02059370.54 was sequence comparison (date:051113).

Source for merge of Syx1A CG10716 was a shared cDNA (date:010720).

Other Comments

Syx1A is required for wls protein-containing exosome release at synaptic boutons.

(Koles et al., 2012)

dsRNA made from templates generated with primers directed against this gene was tested in an RNAi screen to identify genes that regulate Ca²⁺ release.

(Zhang et al., 2006)

dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

(Echard et al., 2004)

Syx1A gene product interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo.

(Wu et al., 1999)

Rop interacts with Syx1A in vivo.

(Wu et al., 1998)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.

(Perrimon et al., 1996)

Germline clonal analysis reveals Syx1A is required in the female germline for oocyte development. Syx1A is required for cell viability in the eye, during wing development and plays a role in membranes of the nervous system.

Syx1A is absolutely required for spontaneous vesicle fusions, for these events are absent in Syx1A null mutants. However, mature, docked synaptic vesicles are observed by EM at release sites in the presynaptic terminals of Syx1A mutant synapses. Therefore, SYX1A functions downstream of vesicle docking and participates in fusion.

(Broadie, 1995)

The Syx1A and n-syb proteins function downstream of vesicle docking.

(Schulze et al., 1995, Schulze et al., 1995)

Identified and cloned using the monoclonal antibody mAb44D5. The protein is localised in the synaptic areas of the CNS and at neuromuscular junctions.

(Cerezo et al., 1995)

The Syx1A gene product plays a role in general secretion and is required for exocytosis from a variety of secretory tissues. Syx1A is absolutely required for evoked neuro- transmission as lack of SYX1A entirely abolishes evoked neurotransmitter release.

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

42854

UniProtKB/Swiss-Prot

•

Q24547

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Target SNARE coiled-coil domain (IPR000727)

Syntaxin, N-terminal (IPR006011)

Syntaxin/epimorphin, conserved site (IPR006012)

t-SNARE (IPR010989)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

LD43943

Linkouts

BioGRID - A database of protein and genetic interactions

•

67788

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0013343

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0013343

DRSC - Results from RNAi screens.

•

FBgn0013343

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0013343

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG33100-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0013343

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

42854

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/neural/syntaxn1.htm

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

42854

modMine - Data generated by the modENCODE project.

•

FBgn0013343

Synonyms & Secondary IDs ( 41 )

Reported As

Symbol Synonym

anon-EST:Gibbs4

anon-WO02059370.54

CG5448

(Verstreken and Bellen, 2000.9.4, Molnar et al., 2006)

CG10716

(Robertson, 2001.5.6, )

CG18615

CG31136

(Perkins et al., 1.15.2009, Khanna et al., 2010)

CT30033

(Xie and Ding, 2000)

Dm Syx1

DmSyx1A

(McLennan, 2001.5.22)

dSyx1

(Xu et al., 2002)

l(3)06737

synt

(Parfitt et al., 1995)

syt-1A

(Verstreken et al., 2003, Drummond et al., 2001, Phillips et al., 2000, Wu et al., 2000, Hiesinger et al., 2005, Wagh et al., 2006, Stowers and Isacoff, 2007, Zeng et al., 2007, Romero et al., 2008, Baqri et al., 2006, Gilestro et al., 2009, Williamson et al., 2010)

Syt1A

Syx

syx1