FlyBase Gene Report: Dmel\TfIIA-S

General Information

Symbol

Dmel\TfIIA-S

Species

D. melanogaster

Name

Transcription-factor-IIA-S

Annotation Symbol

CG5163

Feature Type

protein_coding_gene

FlyBase ID

FBgn0013347

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Snapshot

Transcription-factor-IIA-S (TfIIA-S) encodes one of the basal transcription factors required for the formation of the pre-initiation complex (PIC) at Pol II-transcribed genes. It stabilizes the product of Tbp-TATA box interaction, thus promoting PIC formation. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

TFIIA, dTFIIA-S, F121

Key Links

Genomic Location

Cytogenetic map

95C8-95C8

Sequence location

3R:23,890,388..23,891,060 [-]

Recombination map

3-81

RefSeq locus

NT_033777 REGION:23890388..23891060

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

contributes_to RNA polymerase II general transcription initiation factor activity

inferred from physical interaction with FLYBASE:TfIIA-L; FB:FBgn0011289

(Yokomori et al., 1994)

enables TBP-class protein binding

inferred from physical interaction with FLYBASE:Trf2; FB:FBgn0261793

(Andersen et al., 2017)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables TBP-class protein binding

inferred from biological aspect of ancestor with PANTHER:PTN000103450

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in transcription by RNA polymerase II

inferred from mutant phenotype

(Andersen et al., 2017)

inferred from direct assay

(Yokomori et al., 1994)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in RNA polymerase II preinitiation complex assembly

inferred from biological aspect of ancestor with PANTHER:PTN000103450

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in transcription initiation from RNA polymerase II promoter

inferred from sequence or structural similarity with HGNC:4647

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with SGD:S000001541

(Aoyagi and Wassarman, 2000)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in heterochromatin

inferred from direct assay

(Andersen et al., 2017)

located_in nucleus

inferred from direct assay

(Bernstein et al., 1994, Yokomori et al., 1994)

part_of transcription factor TFIIA complex

inferred from direct assay

(Yokomori et al., 1994)

inferred from physical interaction with FLYBASE:moon; FB:FBgn0030373

(Andersen et al., 2017)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of transcription factor TFIIA complex

inferred from sequence or structural similarity with HGNC:4647

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with SGD:S000001541

(Aoyagi and Wassarman, 2000)

inferred from biological aspect of ancestor with PANTHER:PTN000103450

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

TRANSCRIPTION FACTOR II A

Protein Family (UniProt)

Belongs to the TFIIA subunit 2 family. (P52656)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

TRANSCRIPTION FACTOR II A -: The general transcription factor complex Transcription factor II A (TFIIA) is not required for basal transcription but it stabilizes the TBP-DNA complex. TFIIA is RNA Pol II-specific and stimulates constitutive transcription and activated transcription. (Adapted from PMID:25693126).

Protein Function (UniProtKB)

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation (PubMed:7958898). TFIIA in a complex with TBP mediates transcriptional activity (PubMed:7958898). Part of a rhi-dependent transcription machinery that enables the generation of piRNA precursors from heterochromatin while maintaining the suppression of transposon-encoded promoters and enhancers (PubMed:28847004). Forms a complex with Moonshiner/CG12721 and Trf2 which recruit transcriptional machinery to heterochromatin to initiate the bidirectional transcription of piRNA clusters, by interacting with the RDC (rhi, del and cuff) complex that binds to repressive H3K9me3 marks in the chromatin (PubMed:28847004). This mechanism allows transcription to occur in piRNA clusters despite the lack of proper promoter elements and in the presence of the repressive H3K9me3 mark (PubMed:28847004).

(UniProt, P52656)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\TfIIA-S for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.39

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

TfIIA-S-RA

FBtr0084526

NM_079743

617

106

Additional Transcript Data and Comments

Reported size (kB)

0.6 (longest cDNA)

(Yokomori et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

TfIIA-S-PA

FBpp0083912

12.2

106

8.70

NP_524467

AAF56199

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

12.5 (kD predicted)

(Bernstein et al., 1994)

106 (aa); 12 (kD predicted)

(Yokomori et al., 1994)

Comments

External Data

Subunit Structure (UniProtKB)

TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma (PubMed:7958898). It was originally believed to be a heterotrimer of an alpha (p30), a beta (p20) and a gamma (p14) subunit (PubMed:7958898). Forms a complex with Moonshiner/CG12721 and Trf2 (PubMed:28847004).

(UniProt, P52656)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P52656

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\TfIIA-S using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

TfIIA-S_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium