FB2019_05, released Oct 15, 2019

Gene: Dmel\igl

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General Information
Symbol
Dmel\igl
Species
D. melanogaster
Name
igloo
Annotation Symbol
CG18285
Feature Type
protein_coding_gene
FlyBase ID
FBgn0013467
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Snapshot
igloo (igl) encodes a calmodulin binding protein. [Date last reviewed: 2019-09-19]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
51E3-51E5
Sequence location
2R:15,155,437..15,190,292 [+]
Recombination map
2-74
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
calmodulin binding
Predictions / Assertions
calmodulin binding
Summaries
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\igl or the JBrowse view of Dmel\igl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
igl-RA
FBtr0087361
NM_057712
2514
240
igl-RB
FBtr0087362
NM_057713
1916
122
igl-RC
FBtr0332267
NM_001274059
2713
122
Additional Transcript Data and Comments
Reported size (kB)
2.5, 1.9 (northern blot)
(Neel and Young, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
igl-PA
FBpp0086494
25.0
240
4.98
NP_477060
AAF58168
igl-PB
FBpp0086495
13.0
122
5.03
NP_477061
AAM68523
igl-PC
FBpp0304557
13.0
122
5.03
NP_001260988
AGB93520
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

122 aa isoforms: igl-PB, igl-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
240, 122 (aa); 24, 13 (kD predicted)
(Neel and Young, 1994)
Comments
A yeast assay system was use to show that igl protein binds Calmodulin and that the interaction may be blocked by phosphorylation.
(Neel and Young, 1994)
the long form of igl protein was shown to be phosphorylated by purified protein kinase C in vitro. A yeast assay system was use to show that igl protein binds Calmodulin and that the interaction may be blocked by phosphorylation. Myristoylation of the long form of igl protein was demonstrated in an in vitro reticulocyte translation system.
(Neel and Young, 1994)
One of a couple of protein products.
(FlyBase, 1992-)
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\igl using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (4 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
calmodulin binding
inferred from direct assay
(Neel and Young, 1994)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
calmodulin binding
inferred from biological aspect of ancestor with PANTHER:PTN000075705
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
binding of sperm to zona pellucida
inferred from electronic annotation with InterPro:IPR012105
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
cell cortex
inferred from direct assay
(Benetka et al., 2008)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
membrane
inferred from electronic annotation with InterPro:IPR012105
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 16
dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic head epidermis
(Fisher et al., 2012)
embryonic head sensory system
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
labral sensory complex
(Fisher et al., 2012)
sensory nervous system primordium
(Fisher et al., 2012)
ventral nerve cord
(Fisher et al., 2012)
ventral sensory complex primordium
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- pupal stage
(Neel and Young, 1994)
embryonic stage -- adult stage
(Neel and Young, 1994)
embryonic stage 15 -- 17
central nervous system
(Neel and Young, 1994)
presumptive embryonic/larval peripheral nervous system
(Neel and Young, 1994)
Additional Descriptive Data
The 1.9kb igl transcript is detected throughout development on northern blots. By in situ, it is detected in embryos older than 12 hours and its expression is limited to cells of the CNS and PNS, including cells of the antennomaxillary ganglion and the brain region. Expression appears to be restricted to neurons.
(Neel and Young, 1994)
The 2.5kb igl transcript is detected during embryonic, larval, and pupal development on northern blots. By in situ, it is detected in embryos older than 12 hours and its expression is limited to cells of the CNS and PNS, including cells of the antennomaxillary ganglion and the brain region. Expression appears to be restricted to neurons.
(Neel and Young, 1994)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
• membrane
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
cell cortex
inferred from direct assay
(Benetka et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\igl in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of igl
Transgenic constructs containing regulatory region of igl
Deletions and Duplications ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\NRGN
2 of 15
Yes
Yes
 
Hsap\SPA17
2 of 15
Yes
No
Hsap\GAP43
1 of 15
No
Yes
 
Hsap\LOC100996693
1 of 15
No
Yes
Hsap\PCP4
1 of 15
No
Yes
Hsap\PCP4L1
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Nrgn
2 of 15
Yes
Yes
Mmus\Spa17
2 of 15
Yes
No
Mmus\Gap43
1 of 15
No
Yes
Mmus\Pcp4l1
1 of 15
No
Yes
Mmus\Pcp4
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (4)
Rnor\Nrgn
2 of 13
Yes
Yes
Rnor\Gap43
1 of 13
No
Yes
Rnor\Pcp4
1 of 13
No
Yes
Rnor\Spa17
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (2)
Xtro\pcp4l1
1 of 12
Yes
Yes
Xtro\spa17
1 of 12
Yes
No
Danio rerio (Zebrafish) (5)
Drer\nrgna
2 of 15
Yes
Yes
Drer\spa17
2 of 15
Yes
Yes
Drer\gap43
1 of 15
No
Yes
Drer\pcp4a
1 of 15
No
Yes
Drer\pcp4l1
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\F39H12.3
2 of 15
Yes
No
Cele\ZK1320.13
2 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190H3L )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\igl
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00002197
Drosophila simulans
Dsim\GD11100
Drosophila sechellia
Dsec\GM21592
Drosophila erecta
Dere\GG20500
Drosophila yakuba
Dyak\GE13633
Drosophila ananassae
Dana\GF12313
Drosophila pseudoobscura pseudoobscura
Dpse\GA14874
Drosophila persimilis
Dper\GL10612
Drosophila willistoni
Dwil\GK19703
Drosophila virilis
Dvir\GJ21734
Drosophila mojavensis
Dmoj\GI18717
Drosophila grimshawi
Dgri\GH21112
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150JE5 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17020744
Glossina morsitans
Tsetse fly
Gmor\GMOY008928
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_09905
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL010257
Anopheles darlingi
American malaria mosquito
Adar\ADAC007848
Anopheles gambiae
Malaria mosquito
Agam\AGAP011250
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ005017
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0E29 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA014500
Danaus plexippus
Monarch butterfly
Dple\DPOGS202420-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL010071
Apis florea
Little honeybee
Aflo\2291386900
Apis mellifera
Western honey bee
Amel\GB50780
Apis mellifera
Western honey bee
Amel\GB50783
Apis mellifera
Western honey bee
Amel\GB52581
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP24276
Bombus terrestris
Buff-tailed bumblebee
Bter\15217051
Linepithema humile
Argentine ant
Lhum\LH20609
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000ba3
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG008170
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00939
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_04538
Tribolium castaneum
Red flour beetle
Tcas\TC003798
Pediculus humanus
Human body louse
Phum\PHUM058020
Cimex lectularius
Bed bug
Clec\CLEC005527
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI39258
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_14576
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0E0M )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR009794
Ixodes scapularis
Black-legged tick
Isca\ISCW010446
Tetranychus urticae
Two-spotted spider mite
Turt\tetur01g01730
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_44253
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0UVX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_003740gn
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG14687
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    igl - Atg4a
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - CG11504
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - CG4407
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - CG8654
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - CG8888
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - Cam
    two hybrid
    (Neel and Young, 1994)
    igl - Cyp6a13
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - Dlc90F
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - Fer1HCH
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - FipoQ
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - Hmgs
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - Hsp26
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - Hsp60A
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - ICA69
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - Lst8
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - MRG15
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - ND-75
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - PSR
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - SPE
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - Sil1
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - Tap42
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - hiro
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - mts
    experimental knowledge based
    (Guruharsha et al., 2011)
    igl - nwk
    experimental knowledge based
    (Guruharsha et al., 2011)
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Nsf2
    suppressible
    igl
    (Laviolette et al., 2005)
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-74
    Cytogenetic map
    51E3-51E5
    Sequence location
    2R:15,155,437..15,190,292 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    51E3-51E5
    Limits computationally determined from genome sequence between P{PZ}chn02064 and P{PZ}scb01288
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    51E-51E
    (determined by in situ hybridisation)
    (Neel and Young, 1994)
    Experimentally Determined Recombination Data
    Location
    2-74
    (FlyBase, 2016)
    2-77.7
    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (12)
    Genomic Clones (22)
    cDNA Clones (90)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Additional comments
    Other Comments
    RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
    (Boutros et al., 2004)
    Identified on the basis of sequence similarity to a rat GAP-43.
    (Neel and Young, 1994)
    Origin and Etymology
    Discoverer
    Etymology
    Named "igloo" (igl, invertebrate GAP-43 like) because it encodes proteins related to vertebrate GAP-43.
    (Neel and Young, 1994)
    Identification
    External Crossreferences and Linkouts ( 43 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyMine - An integrated database for Drosophila genomics
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (5)
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (48)