FlyBase Gene Report: Dmel\igl

General Information

Symbol

Dmel\igl

Species

D. melanogaster

Name

igloo

Annotation Symbol

CG18285

Feature Type

protein_coding_gene

FlyBase ID

FBgn0013467

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

igloo (igl) encodes a calmodulin binding protein. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

51E3-51E5

Sequence location

2R:15,155,437..15,190,292 [+]

Recombination map

2-74

RefSeq locus

NT_033778 REGION:15155437..15190292

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables calmodulin binding

inferred from direct assay

(Neel and Young, 1994)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables calmodulin binding

inferred from biological aspect of ancestor with PANTHER:PTN000075705

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Benetka et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

igloo (igl) encodes a calmodulin binding protein. [Date last reviewed: 2019-09-19]

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\igl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7JPS2)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

igl-RA

FBtr0087361

NM_057712

2514

240

igl-RB

FBtr0087362

NM_057713

1916

122

igl-RC

FBtr0332267

NM_001274059

2713

122

Additional Transcript Data and Comments

Reported size (kB)

2.5, 1.9 (northern blot)

(Neel and Young, 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

igl-PA

FBpp0086494

25.0

240

4.98

NP_477060

AAF58168

igl-PB

FBpp0086495

13.0

122

5.03

NP_477061

AAM68523

igl-PC

FBpp0304557

13.0

122

5.03

NP_001260988

AGB93520

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

122 aa isoforms: igl-PB, igl-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

240, 122 (aa); 24, 13 (kD predicted)

(Neel and Young, 1994)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\igl using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

labral sensory complex

(Fisher et al., 2012)

sensory nervous system primordium

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

ventral sensory complex primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

(Neel and Young, 1994)

embryonic stage -- adult stage

(Neel and Young, 1994)

embryonic stage 15 -- 17

central nervous system

(Neel and Young, 1994)

presumptive embryonic/larval peripheral nervous system

(Neel and Young, 1994)

Additional Descriptive Data

The 1.9kb igl transcript is detected throughout development on northern blots. By in situ, it is detected in embryos older than 12 hours and its expression is limited to cells of the CNS and PNS, including cells of the antennomaxillary ganglion and the brain region. Expression appears to be restricted to neurons.

(Neel and Young, 1994)

The 2.5kb igl transcript is detected during embryonic, larval, and pupal development on northern blots. By in situ, it is detected in embryos older than 12 hours and its expression is limited to cells of the CNS and PNS, including cells of the antennomaxillary ganglion and the brain region. Expression appears to be restricted to neurons.

(Neel and Young, 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Benetka et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\igl in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\igl

View exonic expression by tissue for Dmel\igl

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0013467

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36681/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013467

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013467

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013467

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG18285

Flygut - An atlas of the Drosophila adult midgut

FBgn0013467

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\igl

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of igl

Transgenic constructs containing regulatory region of igl

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel7135

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Scer\GAL4^repo

igl^JF03277

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SPA17

3 of 15

Yes

Hsap\GAP43

1 of 15

Yes

Hsap\NRGN

1 of 15

Yes

Hsap\PCP4

1 of 15

Yes

Hsap\PCP4L1

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Spa17

3 of 15

Yes

Mmus\Nrgn

2 of 15

Yes

Mmus\Gap43

1 of 15

Yes

Mmus\Pcp4l1

1 of 15

Yes

Mmus\Pcp4

1 of 15

Yes

Rattus norvegicus (Norway rat) (4)

Rnor\Nrgn

2 of 13

Yes

Rnor\Spa17

2 of 13

Yes

Rnor\Gap43

1 of 13

Yes

Rnor\Pcp4

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\pcp4l1

1 of 12

Yes

Xtro\spa17

1 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\nrgna

2 of 15

Yes

Drer\spa17

2 of 15

Yes

Drer\gap43

1 of 15

Yes

Drer\pcp4a

1 of 15

Yes

Drer\pcp4b

1 of 15

Yes

Drer\pcp4l1

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\F39H12.3

3 of 15

Yes

Cele\ZK1320.13

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

igl^JF03277

ameliorates high grade glioma

modeled by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, btl::Egfr^λ.UAS

(Portela et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SPA17; sperm autoantigenic protein 17

3 of 15

608621

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

igl - Atg4a

experimental knowledge based

(Guruharsha et al., 2011)

igl - CG11504

experimental knowledge based

(Guruharsha et al., 2011)

igl - CG4407

experimental knowledge based

(Guruharsha et al., 2011)

igl - CG8654

experimental knowledge based

(Guruharsha et al., 2011)

igl - CG8888

experimental knowledge based

(Guruharsha et al., 2011)

igl - Cam

two hybrid

(Neel and Young, 1994)

igl - Cyp6a13

experimental knowledge based

(Guruharsha et al., 2011)

igl - Dlc90F

experimental knowledge based

(Guruharsha et al., 2011)

igl - Fer1HCH

experimental knowledge based

(Guruharsha et al., 2011)

igl - FipoQ

experimental knowledge based

(Guruharsha et al., 2011)

igl - Hmgs

experimental knowledge based

(Guruharsha et al., 2011)

igl - Hsp26

experimental knowledge based

(Guruharsha et al., 2011)

igl - Hsp60A

experimental knowledge based

(Guruharsha et al., 2011)

igl - ICA69

experimental knowledge based

(Guruharsha et al., 2011)

igl - JMJD6

experimental knowledge based

(Guruharsha et al., 2011)

igl - Lst8

experimental knowledge based

(Guruharsha et al., 2011)

igl - MRG15

experimental knowledge based

(Guruharsha et al., 2011)

igl - ND-75

experimental knowledge based

(Guruharsha et al., 2011)

igl - SPE

experimental knowledge based

(Guruharsha et al., 2011)

igl - Sil1

experimental knowledge based

(Guruharsha et al., 2011)

igl - Tap42

experimental knowledge based

(Guruharsha et al., 2011)

igl - hiro

experimental knowledge based

(Guruharsha et al., 2011)

igl - mts

experimental knowledge based

(Guruharsha et al., 2011)

igl - nwk

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nsf2

suppressible

igl

(Laviolette et al., 2005)

Pi3K92E|btl::Egfr

suppressible

igl

(Portela et al., 2019)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62421

DroID - A comprehensive database of gene and protein interactions.

FBgn0013467

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0013467

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-74

Cytogenetic map

51E3-51E5

Sequence location

2R:15,155,437..15,190,292 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

51E3-51E5

Limits computationally determined from genome sequence between P{PZ}chn⁰²⁰⁶⁴ and P{PZ}scb⁰¹²⁸⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

51E-51E

(determined by in situ hybridisation)

(Neel and Young, 1994)

Experimentally Determined Recombination Data

Location

2-74

(FlyBase, 2016)

2-77.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

27748

w¹¹¹⁸; Mi{ET1}igl^MB07097

85212

w¹¹¹⁸; PBac{WH}igl^f03185

29598

y¹ v¹; P{TRiP.JF03277}attP2

28096

y¹ w^*; P{EP}igl^G17971

28426

y¹ w^*; P{EP}igl^G19669

34310

y¹ w^*; Mi{MIC}igl^MI02290

59097

y¹ w^*; Mi{MIC}igl^MI12785

60527

y¹ w^67c23; Mi{PT-GFSTF.1}igl^{MI02290-GFSTF.1}

76744

y¹ w^*; Mi{Trojan-GAL4.1}igl^{MI12785-TG4.1}/SM6a

Kyoto

203481

y¹ w^67c23; P{GSV6}igl^GS11641/SM1

104644

y^* w^*; P{GawB}igl^NP4240 / CyO, P{UAS-lacZ.UW14}UW14

VDRC

v100159

P{KK105109}VIE-260B

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (90)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH28577

Other clones

DPiM_GH28577

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0013467

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0013467

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36681

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Identified on the basis of sequence similarity to a rat GAP-43.

(Neel and Young, 1994)

Origin and Etymology

Discoverer

Etymology

Named "igloo" (igl, invertebrate GAP-43 like) because it encodes proteins related to vertebrate GAP-43.

(Neel and Young, 1994)

Identification

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36681

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7JPS2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7JPS2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0013467

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0013467

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36681/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013467

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013467

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013467

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG18285

Flygut - An atlas of the Drosophila adult midgut

FBgn0013467

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0013467

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36681

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG18285

MARRVEL_MODEL - MARRVEL (model organism gene)

36681

modMine - A data warehouse for the modENCODE project

FBgn0013467

Linkouts

BioGRID - A database of protein and genetic interactions.

62421

DroID - A comprehensive database of gene and protein interactions.

FBgn0013467

DRSC - Results frm RNAi screens

FBgn0013467

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0013467

FlyMine - An integrated database for Drosophila genomics

FBgn0013467

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG18285

(Keleman et al., 2009.8.5, Perkins et al., 2009, Franke et al., 2006, Laviolette et al., 2005)

Igl

(Schauer et al., 2013)

igl

(Yildirim et al., 2019, Lee et al., 2018, Solis et al., 2017, Gene Disruption Project members, 2016-, Gene Disruption Project members, 2015-, Hamilton et al., 2015, Sytnikova et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Jordan et al., 2012, Murray et al., 2012, Franke et al., 2006, Laviolette et al., 2005)

Name Synonyms

igloo

(Benetka et al., 2008, Neel, 1995.1.5, Neel, 1995.1.5)

invertebrate GAP-43-like

Secondary FlyBase IDs

Datasets (6)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (6)