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Gene Dmel\MED20

General Information
Symbol	Dmel\MED20	Species	D. melanogaster
Name	Mediator complex subunit 20	Annotation symbol	CG18780
Feature type	protein_coding_gene	FlyBase ID	FBgn0013531
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	Trfp, l(2)28DE
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	28E1-28E1	Sequence location	2L:8,071,809..8,072,844 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Mediator complex subunit 20 is referred to in FlyBase by the symbol Dmel\MED20 (CG18780, FBgn0013531). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: transcription coactivator activity. There is experimental evidence that it is involved in the biological process: regulation of transcription from RNA polymerase II promoter. 6 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Mediator complex, subunit Med20. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1641105_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of MED20. Gene sequence location is 2L:8071809..8072844.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features BKN21317 HFA03463
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 28E1-28E1   Limits computationally determined from genome sequence between P{EP}poeEP737 and P{lacW}l(2)k16919k16919&P{lacW}Bsgk09030
Experimentally Determined Cytological Location
Cytogenetic map Notes References 28D-28E     (Crowley, 1997.2.3) 28D-28E     (Crowley et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\MED20 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) MED20-RA FBtr0079544  NM_079924   1036   252
Additional Transcript Data & Comments
Reported size (kB)	0.95 (longest cDNA) (Vallejo and Leon, 1989)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein MED20-PA   FBpp0079166   27.9   252   7.74     NP_524663   AAF52599
Additional Polypeptide Data & Comments
Reported size (kDa)	252 (aa) (Crowley, 1997.7.14)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med20 (IPR013921)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF52599   BE975967   BE976051   BI375943   BI639667   BP543851   BP543852   BP545634   BP545635   BP559266   BP559267   BT003473 AAO39476   CZ483431   CZ483432   EL873789   Y10975 CAA71871
UniProtKB/Swiss-Prot	P91641
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference spermatogenesis & adult stage   testis   (Vallejo and Leon, 1989) spermatocyte   (Vallejo and Leon, 1989)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0013531    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0013531 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0013531 expression level Developmental Stage   Expression Level embryo 00-02hr    2132 embryo 02-04hr    1464 embryo 04-06hr    2373 embryo 06-08hr    1823 embryo 08-10hr    1547 embryo 10-12hr    1312 embryo 12-14hr    755 embryo 14-16hr    659 embryo 16-18hr    537 embryo 18-20hr    290 embryo 20-22hr    280 embryo 22-24hr    307 larva L1    616 larva L2    532 larva L3 12hr old    507 larva L3 puffstage 1-2    517 larva L3 puffstage 3-6    731 larva L3 puffstage 7-9    822 white prepupae new    748 white prepupae 12hr    832 white prepupae 24hr    1049 pupae 2d postWPP    752 pupae 3d postWPP    385 pupae 4d postWPP    324 adult male 01day    462 adult male 05day    673 adult male 30day    764 adult female 01day    766 adult female 05day    1096 adult female 30day    1290 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2132) embryo 02-04hr  (1464) embryo 04-06hr  (2373) embryo 06-08hr  (1823) embryo 08-10hr  (1547) embryo 10-12hr  (1312) embryo 12-14hr  (755) embryo 14-16hr  (659) embryo 16-18hr  (537) embryo 18-20hr  (290) embryo 20-22hr  (280) embryo 22-24hr  (307) larva L1  (616) larva L2  (532) larva L3 12hr old  (507) larva L3 puffstage 1-2  (517) larva L3 puffstage 3-6  (731) larva L3 puffstage 7-9  (822) white prepupae new  (748) white prepupae 12hr  (832) white prepupae 24hr  (1049) pupae 2d postWPP  (752) pupae 3d postWPP  (385) pupae 4d postWPP  (324) adult male 01day  (462) adult male 05day  (673) adult male 30day  (764) adult female 01day  (766) adult female 05day  (1096) adult female 30day  (1290) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2132) embryo 02-04hr  1464 embryo 04-06hr  (2373) embryo 06-08hr  (1823) embryo 08-10hr  (1547) embryo 10-12hr    1312 embryo 12-14hr    755 embryo 14-16hr    659 embryo 16-18hr    537 embryo 18-20hr    290 embryo 20-22hr    280 embryo 22-24hr    307 larva L1    616 larva L2    532 larva L3 12hr old    507 larva L3 puffstage 1-2    517 larva L3 puffstage 3-6    731 larva L3 puffstage 7-9    822 white prepupae new    748 white prepupae 12hr    832 white prepupae 24hr    1049 pupae 2d postWPP    752 pupae 3d postWPP    385 pupae 4d postWPP    324 adult male 01day    462 adult male 05day    673 adult male 30day    764 adult female 01day    766 adult female 05day    1096 adult female 30day    1290 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2132 embryo 02-04hr    1464 embryo 04-06hr    2373 embryo 06-08hr    1823 embryo 08-10hr    1547 embryo 10-12hr    1312 embryo 12-14hr    755 embryo 14-16hr    659 embryo 16-18hr    537 embryo 18-20hr    290 embryo 20-22hr    280 embryo 22-24hr    307 larva L1    616 larva L2    532 larva L3 12hr old    507 larva L3 puffstage 1-2    517 larva L3 puffstage 3-6    731 larva L3 puffstage 7-9    822 white prepupae new    748 white prepupae 12hr    832 white prepupae 24hr    1049 pupae 2d postWPP    752 pupae 3d postWPP    385 pupae 4d postWPP    324 adult male 01day    462 adult male 05day    673 adult male 30day    764 adult female 01day    766 adult female 05day    1096 adult female 30day    1290 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2132 embryo 02-04hr    1464 embryo 04-06hr    2373 embryo 06-08hr    1823 embryo 08-10hr    1547 embryo 10-12hr    1312 embryo 12-14hr    755 embryo 14-16hr    659 embryo 16-18hr    537 embryo 18-20hr    290 embryo 20-22hr    280 embryo 22-24hr    307 larva L1    616 larva L2    532 larva L3 12hr old    507 larva L3 puffstage 1-2    517 larva L3 puffstage 3-6    731 larva L3 puffstage 7-9    822 white prepupae new    748 white prepupae 12hr    832 white prepupae 24hr    1049 pupae 2d postWPP    752 pupae 3d postWPP    385 pupae 4d postWPP    324 adult male 01day    462 adult male 05day    673 adult male 30day    764 adult female 01day    766 adult female 05day    1096 adult female 30day    1290 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0013531 expression level Developmental Stage   Expression Level embryo 00-02hr    2132 embryo 02-04hr    1464 embryo 04-06hr    2373 embryo 06-08hr    1823 embryo 08-10hr    1547 embryo 10-12hr    1312 embryo 12-14hr    755 embryo 14-16hr    659 embryo 16-18hr    537 embryo 18-20hr    290 embryo 20-22hr    280 embryo 22-24hr    307 larva L1    616 larva L2    532 larva L3 12hr old    507 larva L3 puffstage 1-2    517 larva L3 puffstage 3-6    731 larva L3 puffstage 7-9    822 white prepupae new    748 white prepupae 12hr    832 white prepupae 24hr    1049 pupae 2d postWPP    752 pupae 3d postWPP    385 pupae 4d postWPP    324 adult male 01day    462 adult male 05day    673 adult male 30day    764 adult female 01day    766 adult female 05day    1096 adult female 30day    1290 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2132) embryo 02-04hr  (1464) embryo 04-06hr  (2373) embryo 06-08hr  (1823) embryo 08-10hr  (1547) embryo 10-12hr  (1312) embryo 12-14hr  (755) embryo 14-16hr  (659) embryo 16-18hr  (537) embryo 18-20hr  (290) embryo 20-22hr  (280) embryo 22-24hr  (307) larva L1  (616) larva L2  (532) larva L3 12hr old  (507) larva L3 puffstage 1-2  (517) larva L3 puffstage 3-6  (731) larva L3 puffstage 7-9  (822) white prepupae new  (748) white prepupae 12hr  (832) white prepupae 24hr  (1049) pupae 2d postWPP  (752) pupae 3d postWPP  (385) pupae 4d postWPP  (324) adult male 01day  (462) adult male 05day  (673) adult male 30day  (764) adult female 01day  (766) adult female 05day  (1096) adult female 30day  (1290) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  2132 embryo 02-04hr  1464 embryo 04-06hr  2373 embryo 06-08hr  1823 embryo 08-10hr  1547 embryo 10-12hr    1312 embryo 12-14hr    755 embryo 14-16hr    659 embryo 16-18hr    537 embryo 18-20hr    290 embryo 20-22hr    280 embryo 22-24hr    307 larva L1    616 larva L2    532 larva L3 12hr old    507 larva L3 puffstage 1-2    517 larva L3 puffstage 3-6    731 larva L3 puffstage 7-9    822 white prepupae new    748 white prepupae 12hr    832 white prepupae 24hr    1049 pupae 2d postWPP    752 pupae 3d postWPP    385 pupae 4d postWPP    324 adult male 01day    462 adult male 05day    673 adult male 30day    764 adult female 01day    766 adult female 05day    1096 adult female 30day    1290 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2132 embryo 02-04hr    1464 embryo 04-06hr    2373 embryo 06-08hr    1823 embryo 08-10hr    1547 embryo 10-12hr    1312 embryo 12-14hr    755 embryo 14-16hr    659 embryo 16-18hr    537 embryo 18-20hr    290 embryo 20-22hr    280 embryo 22-24hr    307 larva L1    616 larva L2    532 larva L3 12hr old    507 larva L3 puffstage 1-2    517 larva L3 puffstage 3-6    731 larva L3 puffstage 7-9    822 white prepupae new    748 white prepupae 12hr    832 white prepupae 24hr    1049 pupae 2d postWPP    752 pupae 3d postWPP    385 pupae 4d postWPP    324 adult male 01day    462 adult male 05day    673 adult male 30day    764 adult female 01day    766 adult female 05day    1096 adult female 30day    1290 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2132 embryo 02-04hr    1464 embryo 04-06hr    2373 embryo 06-08hr    1823 embryo 08-10hr    1547 embryo 10-12hr    1312 embryo 12-14hr    755 embryo 14-16hr    659 embryo 16-18hr    537 embryo 18-20hr    290 embryo 20-22hr    280 embryo 22-24hr    307 larva L1    616 larva L2    532 larva L3 12hr old    507 larva L3 puffstage 1-2    517 larva L3 puffstage 3-6    731 larva L3 puffstage 7-9    822 white prepupae new    748 white prepupae 12hr    832 white prepupae 24hr    1049 pupae 2d postWPP    752 pupae 3d postWPP    385 pupae 4d postWPP    324 adult male 01day    462 adult male 05day    673 adult male 30day    764 adult female 01day    766 adult female 05day    1096 adult female 30day    1290 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0013531    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0013531 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0013531 expression level Tissue   Expression Level Larval Central Nervous System    120.85 Larval Midgut    25.4 Larval Hindgut    36 Larval Malpighian Tubules    65.3 Larval Fat Body    43.2 Larval Salivary Gland    51.2 Larval Trachea    55.55 Larval Carcass    27.375 Adult Head    43.8 Adult Eye    25.425 Adult Brain    52.2 Adult Thoracic-Abdominal Ganglion    46.9 Adult Crop    50.5 Adult Midgut    53.2 Adult Hindgut    60.6 Adult Malpighian Tubules    39.7 Adult Fat Body    25.5 Adult Salivary Gland no informative data Adult Heart    25.5 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    32.8 Adult Ovary    163.4 Adult Testis    70.4 Adult Male Accessory Gland    55.5 Adult Carcass    36.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    120.85 Larval Midgut    25.4 Larval Hindgut    36 Larval Malpighian Tubules    65.3 Larval Fat Body    43.2 Larval Salivary Gland    51.2 Larval Trachea    55.55 Larval Carcass    27.375 Adult Head    43.8 Adult Eye    25.425 Adult Brain    52.2 Adult Thoracic-Abdominal Ganglion    46.9 Adult Crop    50.5 Adult Midgut    53.2 Adult Hindgut    60.6 Adult Malpighian Tubules    39.7 Adult Fat Body    25.5 Adult Salivary Gland no informative data Adult Heart    25.5 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    32.8 Adult Ovary    163.4 Adult Testis    70.4 Adult Male Accessory Gland    55.5 Adult Carcass    36.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    120.85 Larval Midgut    25.4 Larval Hindgut    36 Larval Malpighian Tubules    65.3 Larval Fat Body    43.2 Larval Salivary Gland    51.2 Larval Trachea    55.55 Larval Carcass    27.375 Adult Head    43.8 Adult Eye    25.425 Adult Brain    52.2 Adult Thoracic-Abdominal Ganglion    46.9 Adult Crop    50.5 Adult Midgut    53.2 Adult Hindgut    60.6 Adult Malpighian Tubules    39.7 Adult Fat Body    25.5 Adult Salivary Gland no informative data Adult Heart    25.5 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    32.8 Adult Ovary    163.4 Adult Testis    70.4 Adult Male Accessory Gland    55.5 Adult Carcass    36.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    120.85 Larval Midgut    25.4 Larval Hindgut    36 Larval Malpighian Tubules    65.3 Larval Fat Body    43.2 Larval Salivary Gland    51.2 Larval Trachea    55.55 Larval Carcass    27.375 Adult Head    43.8 Adult Eye    25.425 Adult Brain    52.2 Adult Thoracic-Abdominal Ganglion    46.9 Adult Crop    50.5 Adult Midgut    53.2 Adult Hindgut    60.6 Adult Malpighian Tubules    39.7 Adult Fat Body    25.5 Adult Salivary Gland no informative data Adult Heart    25.5 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    32.8 Adult Ovary    163.4 Adult Testis    70.4 Adult Male Accessory Gland    55.5 Adult Carcass    36.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0013531 expression level Tissue   Expression Level Larval Central Nervous System    120.85 Larval Midgut    25.4 Larval Hindgut    36 Larval Malpighian Tubules    65.3 Larval Fat Body    43.2 Larval Salivary Gland    51.2 Larval Trachea    55.55 Larval Carcass    27.375 Adult Head    43.8 Adult Eye    25.425 Adult Brain    52.2 Adult Thoracic-Abdominal Ganglion    46.9 Adult Crop    50.5 Adult Midgut    53.2 Adult Hindgut    60.6 Adult Malpighian Tubules    39.7 Adult Fat Body    25.5 Adult Salivary Gland no informative data Adult Heart    25.5 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    32.8 Adult Ovary    163.4 Adult Testis    70.4 Adult Male Accessory Gland    55.5 Adult Carcass    36.1 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    120.85 Larval Midgut    25.4 Larval Hindgut    36 Larval Malpighian Tubules    65.3 Larval Fat Body    43.2 Larval Salivary Gland    51.2 Larval Trachea    55.55 Larval Carcass    27.375 Adult Head    43.8 Adult Eye    25.425 Adult Brain    52.2 Adult Thoracic-Abdominal Ganglion    46.9 Adult Crop    50.5 Adult Midgut    53.2 Adult Hindgut    60.6 Adult Malpighian Tubules    39.7 Adult Fat Body    25.5 Adult Salivary Gland no informative data Adult Heart    25.5 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    32.8 Adult Ovary    163.4 Adult Testis    70.4 Adult Male Accessory Gland    55.5 Adult Carcass    36.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    120.85 Larval Midgut    25.4 Larval Hindgut    36 Larval Malpighian Tubules    65.3 Larval Fat Body    43.2 Larval Salivary Gland    51.2 Larval Trachea    55.55 Larval Carcass    27.375 Adult Head    43.8 Adult Eye    25.425 Adult Brain    52.2 Adult Thoracic-Abdominal Ganglion    46.9 Adult Crop    50.5 Adult Midgut    53.2 Adult Hindgut    60.6 Adult Malpighian Tubules    39.7 Adult Fat Body    25.5 Adult Salivary Gland no informative data Adult Heart    25.5 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    32.8 Adult Ovary    163.4 Adult Testis    70.4 Adult Male Accessory Gland    55.5 Adult Carcass    36.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    120.85 Larval Midgut    25.4 Larval Hindgut    36 Larval Malpighian Tubules    65.3 Larval Fat Body    43.2 Larval Salivary Gland    51.2 Larval Trachea    55.55 Larval Carcass    27.375 Adult Head    43.8 Adult Eye    25.425 Adult Brain    52.2 Adult Thoracic-Abdominal Ganglion    46.9 Adult Crop    50.5 Adult Midgut    53.2 Adult Hindgut    60.6 Adult Malpighian Tubules    39.7 Adult Fat Body    25.5 Adult Salivary Gland no informative data Adult Heart    25.5 Adult VirginFemale Spermatheca    25.7 Adult InseminatedFemale Spermatheca    32.8 Adult Ovary    163.4 Adult Testis    70.4 Adult Male Accessory Gland    55.5 Adult Carcass    36.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    43.8   NA  Eye    25.425   NA  Brain    52.2   120.85  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    46.9   NA  Crop    50.5   25.4  Midgut    53.2   36  Hindgut    60.6   65.3  Malpighian Tubules    39.7   43.2  Fat Body    25.5   51.2  Salivary Gland    no informative data   NA  Heart    25.5   55.55  Trachea    NA   NA  VirginFemale Spermatheca    25.7   NA  InseminatedFemale Spermatheca    32.8   NA  Ovary    163.4   NA  Testis    70.4   NA  Male Accessory Gland    55.5   27.375  Carcass    36.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013531 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18780-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | larval stage | recessive MED20C6R20 lethal | recessive MED20C6R20 Phenotype manifest in Allele
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of MED20 Class Mutagen Stocks Known lesion MED20f00955 piggyBac transposase 3 -- MED20C6R20 loss of function allele Delta2-3 1 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of MED20 Class Mutagen Stocks Known lesion MED20GD9652 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes MED20HMS01051 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MED20a.cKa in vitro construct - RNAi 0 Yes MED20cKa in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel7034 (Ryder, 2004.4.26) Df(2L)Trf-C6R31 (Crowley et al., 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9652} NA P{TRiP.HMS01051} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}MED20f00955 NA insertion of mobile activating element P{EPg}HP26217 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function
CV term Evidence References
contributes_to transcription coactivator activity inferred from direct assay (Gu et al., 2002)
Biological Process
CV term Evidence References
regulation of transcription from RNA polymerase II promoter inferred from direct assay (Gu et al., 2002)
Cellular Component
CV term Evidence References
mediator complex inferred from direct assay (Gu et al., 2002) nucleus inferred from direct assay (Gu et al., 2002)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
DNA-directed RNA polymerase activity inferred from sequence or structural similarity with UniProtKB:Q9H944 AND non-traceable author statement (assigned by UniProtKB) (Swiss-Prot Project Members, 2003.9.15) RNA polymerase II transcription cofactor activity inferred from electronic annotation with InterPro:IPR013921 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Boube et al., 2000)
Biological Process
CV term Evidence References
transcription, DNA-dependent inferred from sequence or structural similarity with UniProtKB:Q9H944 AND non-traceable author statement (assigned by UniProtKB) (Swiss-Prot Project Members, 2003.9.15) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity (Boube et al., 2000)
Cellular Component
CV term Evidence References
mediator complex inferred from sequence or structural similarity (Boube et al., 2000) inferred from sequence or structural similarity with UniProtKB:Q9H944 (assigned by UniProtKB) (Swiss-Prot Project Members, 2003.9.15) nucleus non-traceable author statement (assigned by UniProtKB) (Swiss-Prot Project Members, 2003.9.15)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions MED20 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68741 DroID - A comprehensive database of gene and protein interactions. • FBgn0013531 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43952
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22602 Dere\GG23479 Dgri\GH12797 Dmoj\GI15952 Dper\GL19867 Dpse\GA28197 Dsec\GM13189 Dsim\GD22443 Dvir\GJ18844 Dwil\GK16399 Dyak\GE11200
Curated drosophilid orthologs
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL001260-PA Aedes aegypti (mosquito) AGAP005604-PA Anopheles gambiae (mosquito) XP_001121318.1 Apis mellifera (honey bee) XP_001946614.1 Acyrthosiphon pisum (pea aphid) XP_001949764.1 Acyrthosiphon pisum (pea aphid) 277692 Branchiostoma floridae (lancelet) 286465 Branchiostoma floridae (lancelet) BGIBMGA013918-PA Bombyx mori (silk worm) ENSBTAP00000013323 Bos taurus (bovine) CN05660 Caenorhabditis brenneri (small nematode) CE28102 Caenorhabditis elegans (worm) ENSCAFP00000002348 Canis familiaris (dog) JA10421 Caenorhabditis japonica (nematode) CPIJ002301 Culex pipens (mosquito) ENSCPOP00000003971 Cavia porcellus (guinea pig) RP27769 Caenorhabditis remanei (nematode) 220200 Capitella spl (polychaete worm) DDB0266923 Dictyostelium discoideum (amoeba) 59294 Daphnia pulex (daphnia) ENSDARP00000067637 Danio rerio (zebra fish) ENSECAP00000017646 Equus caballus (horse) ENSGACP00000001367 Gymnopilus aculeatus (mushroom) ENSGALP00000005490 Gallus gallus (chicken) 63878 Hypsipyla robusta (cedar shoot borer) ENSP00000265350 Homo sapiens (human) ISCW021675-PA Ixodes scapularis (deer tick) 153713 Lottia gigantea (owl limpet) ENSMODP00000016028 Monodelphis domestica (gray opossum) ENSMMUP00000033701 Macaca mulatta (rhesus monkey) ENSMUSP00000024778 Mus musculus (house mouse) 92443 Nematostella vectensis (starlet anemone) XP_001600958.1 Nasonia vitripennis (wasp) ENSORLP00000000318 Oryzias latipes (japanese killifish) PHUM003212-PA Pediculus humanus (human body louse) ENSPPYP00000018572 Pongo pygmaeus (orangutan) ENSPTRP00000031034 Pan troglodytes (chimpanzee) ENSRNOP00000018523 Rattus norvegicus (norway rat) Smp_042780 Schistosoma mansoni (trematode parasite) 55346 Trichoplax adhaerens (metazoa) XP_001812951.1 Tribolium castaneum (red flour beetle) ENSTNIP00000017675 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000022390 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000025893 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11302 LH19090 ISCW021675 FBgn0229022 FBgn0218401 FBgn0205990 FBgn0193846 FBgn0168120 JGI_V11_59294 FBgn0249560 FBgn0157465 FBgn0138701 FBgn0120276 FBgn0115626 FBgn0099596 CPIJ002301 BGIBMGA013918 ACYPI009722 ACYPI003096 GB30214 GB19813 MED20_ANOGA MED20_AEDAE PB25995 PHUM387850 GLEAN_13006
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	4967 y1 w*; MED20C6R20/CyO 18394 w1118; PBac{WH}MED20f00955/CyO 34577 y1 sc* v1; P{TRiP.HMS01051}attP2
Harvard	- PBac{WH}MED20f00955
Kyoto	114524 yd2 w1118 P{ey-FLP.N}2; PBac{WH}MED20f00955 P{neoFRT}40A/CyO y+
VDRC	v52483 w1118; P{GD9652}v52483 v52484 w1118; P{GD9652}v52484/TM3
Genomic Clones ( 1 )
	BACR08I01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 8 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE63851
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD22312
Other clones	bs46c03 bs46f10 FGC022G03 FGC032D05 FGM319H04 IP13870
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013531 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43952
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: MED20 Trfp (Bourbon et al., 2004)
Source for database merge of	Source for merge of: Trfp CG18267 (Bayraktaroglu, 2000.4.3) Source for merge of: Trfp CG18780 (Matthews, 2001.12.6)
Additional comments
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Marr et al., 2006) Transcription unit defined as part of a molecular analysis of Trf. (Crowley et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079924 RefSeq (Proteins) • NP_524663 DDBJ / EMBL / GenBank • AAF52599 AAO39476 BI375943 BT003473 CAA71871 CZ483431 CZ483432 Y10975 Entrez Gene - A searchable database of RefSeq genes. • 43952 UniProtKB/Swiss-Prot • P91641
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med20 (IPR013921)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68741 DroID - A comprehensive database of gene and protein interactions. • FBgn0013531 DRSC - Results from RNAi screens. • FBgn0013531 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013531 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18780-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013531 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43952 InParanoid A subset of ortholog calls from InParanoid. • AAEL001260-PA Aedes aegypti (mosquito) AGAP005604-PA Anopheles gambiae (mosquito) XP_001121318.1 Apis mellifera (honey bee) XP_001946614.1 Acyrthosiphon pisum (pea aphid) XP_001949764.1 Acyrthosiphon pisum (pea aphid) 277692 Branchiostoma floridae (lancelet) 286465 Branchiostoma floridae (lancelet) BGIBMGA013918-PA Bombyx mori (silk worm) ENSBTAP00000013323 Bos taurus (bovine) CN05660 Caenorhabditis brenneri (small nematode) CE28102 Caenorhabditis elegans (worm) ENSCAFP00000002348 Canis familiaris (dog) JA10421 Caenorhabditis japonica (nematode) CPIJ002301 Culex pipens (mosquito) ENSCPOP00000003971 Cavia porcellus (guinea pig) RP27769 Caenorhabditis remanei (nematode) 220200 Capitella spl (polychaete worm) DDB0266923 Dictyostelium discoideum (amoeba) 59294 Daphnia pulex (daphnia) ENSDARP00000067637 Danio rerio (zebra fish) ENSECAP00000017646 Equus caballus (horse) ENSGACP00000001367 Gymnopilus aculeatus (mushroom) ENSGALP00000005490 Gallus gallus (chicken) 63878 Hypsipyla robusta (cedar shoot borer) ENSP00000265350 Homo sapiens (human) ISCW021675-PA Ixodes scapularis (deer tick) 153713 Lottia gigantea (owl limpet) ENSMODP00000016028 Monodelphis domestica (gray opossum) ENSMMUP00000033701 Macaca mulatta (rhesus monkey) ENSMUSP00000024778 Mus musculus (house mouse) 92443 Nematostella vectensis (starlet anemone) XP_001600958.1 Nasonia vitripennis (wasp) ENSORLP00000000318 Oryzias latipes (japanese killifish) PHUM003212-PA Pediculus humanus (human body louse) ENSPPYP00000018572 Pongo pygmaeus (orangutan) ENSPTRP00000031034 Pan troglodytes (chimpanzee) ENSRNOP00000018523 Rattus norvegicus (norway rat) Smp_042780 Schistosoma mansoni (trematode parasite) 55346 Trichoplax adhaerens (metazoa) XP_001812951.1 Tribolium castaneum (red flour beetle) ENSTNIP00000017675 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000022390 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000025893 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43952 modMine - Data generated by the modENCODE project. • FBgn0013531 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11302 LH19090 ISCW021675 FBgn0229022 FBgn0218401 FBgn0205990 FBgn0193846 FBgn0168120 JGI_V11_59294 FBgn0249560 FBgn0157465 FBgn0138701 FBgn0120276 FBgn0115626 FBgn0099596 CPIJ002301 BGIBMGA013918 ACYPI009722 ACYPI003096 GB30214 GB19813 MED20_ANOGA MED20_AEDAE PB25995 PHUM387850 GLEAN_13006
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	CG18267   CG18780 (Ni et al., 2010.12.1) dTrap26 (Boube et al., 2000) dTrfp (Gu et al., 2002) l(2)28DE (Crowley, 1994.1.18, Crowley et al., 1993, ) Med20 (Kim et al., 2004, Kim and Lis, 2005) MED20 (Marr et al., 2006) med20 (Terriente-Félix et al., 2010) p29 (Park et al., 2001) Srb2/TRFP (Boube et al., 2002) Tmr (Crowley, 1998.8.9, ) Trfp (Bourbon et al., 2004, Crowley, 1998, Matthews, 2001.12.6, Yager et al., 2001, Crowley, 1997.7.14, Gu et al., 2002, Guglielmi et al., 2004, Gim et al., 2001) TrfP (Herr and Harris, 2004)
Name Synonym	lethal(2)28DE (Crowley et al., 1993, ) Mediator (Park et al., 2001) Mediator complex subunit 20 (Bourbon et al., 2004) Transcription mediator-related (Crowley, 1998.8.9, ) Trf-proximal
Secondary FlyBase IDs
	FBgn0031983 FBgn0042121
References ( 39 )
Generate a list of	All references relating to this gene ( 39 )
List References by type	Research paper  ( 13 ) Supplementary material  ( 2 ) Review  ( 2 ) Personal communication to FlyBase  ( 13 ) FlyBase analysis  ( 1 ) Letter  ( 2 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 3 )
Recent research papers ( 1 )
	Terriente-Félix et al., 2010, Genetics 185(2): 671--684 Identification of genes affecting wing patterning through a loss-of-function mutagenesis screen and characterization of med15 function during wing development. [FBrf0211230] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...