FlyBase Gene Report: Dmel\not

General Information

Symbol

Dmel\not

Species

D. melanogaster

Name

non-stop

Annotation Symbol

CG4166

Feature Type

protein_coding_gene

FlyBase ID

FBgn0013717

Gene Model Status

Stock Availability

12 publicly available

Enzyme Name (EC)

Ubiquitinyl hydrolase 1 (3.4.19.12)

Gene Summary

non-stop (not) encodes a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) chromatin modifying complex. It deubiquitinates the product of His2B at active promoters, thereby promoting transcription elongation. It was first identified for its role in neural development in the optic lobe. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

l(3)02069

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:18,618,305..18,622,129 [-]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:18618305..18622129

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:Sgf11; FB:FBgn0036804

(Weake et al., 2008)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables thiol-dependent deubiquitinase

inferred from electronic annotation with InterPro:IPR001394, InterPro:IPR018200

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence model

(Poeck et al., 2001)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR001607

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in axon target recognition

inferred from mutant phenotype

(Martin et al., 1995)

involved_in chromatin organization

inferred from mutant phenotype

(Zhao et al., 2008)

involved_in glial cell development

inferred from mutant phenotype

(Poeck et al., 2001)

involved_in glial cell migration

inferred from mutant phenotype

(Poeck et al., 2001)

involved_in histone deubiquitination

inferred from direct assay

(Mohan et al., 2014)

inferred from mutant phenotype

(Weake et al., 2008)

involved_in positive regulation of transcription, DNA-templated

inferred from mutant phenotype

(assigned by UniProt )

(Zhao et al., 2008)

involved_in protein deubiquitination

inferred from mutant phenotype

(Poeck et al., 2001)

involved_in regulation of transcription, DNA-templated

inferred from mutant phenotype

(Weake et al., 2008)

involved_in ubiquitin-dependent protein catabolic process

inferred from genetic interaction with FLYBASE:Prosβ6; FB:FBgn0002284

(Poeck et al., 2001)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

part_of DUBm complex

inferred from direct assay

(Mohan et al., 2014)

located_in nucleus

inferred from direct assay

(Weake et al., 2011)

part_of SAGA complex

inferred from direct assay

(Weake et al., 2011, Weake et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

USP DEUBIQUITINASES

Protein Family (UniProt)

Belongs to the peptidase C19 family. UBP8 subfamily. (Q9VVR1)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

-

Predictions / Assertions

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) (3.4.19.12)

Summaries

Gene Snapshot

non-stop (not) encodes a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) chromatin modifying complex. It deubiquitinates the product of His2B at active promoters, thereby promoting transcription elongation. It was first identified for its role in neural development in the optic lobe. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

USP DEUBIQUITINASES -: The Ubiquitin Specific Proteases (USP) is the largest sub-family of deubiquitinases. They are cysteine proteases and catalyze the removal of ubiquitin from ubiquitin chains and ubiquitinated proteins. USP deubiquitinases belong to MEROPS family C19. (Adapted from PMID:19626045).
SAGA COMPLEX -: The Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex is a chromatin modifying complex. SAGA can acetylate and deubiquitinate histone residues. Also SAGA has been shown to regulate the transcriptional activation of a subset of genes. (Adapted from FBrf0228923, FBrf0219841 and FBrf0233349).

Protein Function (UniProtKB)

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of histone H2B, thereby acting as a coactivator in a large subset of genes. Required to counteract heterochromatin silencing. Controls the development of neuronal connectivity in visual system by being required for accurate axon targeting in the optic lobe. Required for expression of ecdysone-induced genes such as br/broad.

(UniProt, Q9VVR1)

Summary (Interactive Fly)

ubiquitin-specific protease involved in glial development - effects axon guidance

Interactive Fly

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\not for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VVR1)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0290259

2488

496

FBtr0346438

2989

496

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0288698

56.4

496

8.03

FBpp0312097

56.4

496

8.03

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

496 aa isoforms: not-PB, not-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the SAGA transcription coactivator-HAT complex, at least composed of Ada2b, not/nonstop, Pcaf/Gcn5, Sgf11 and Spt3.

(UniProt, Q9VVR1)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\not using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

not_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic/larval optic lobe | faint | restricted

(Poeck et al., 2001)

lamina anlage | faint | restricted

(Poeck et al., 2001)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of DUBm complex

inferred from direct assay

(Mohan et al., 2014)

located_in nucleus

inferred from direct assay

(Weake et al., 2011)

part_of SAGA complex

inferred from direct assay

(Weake et al., 2011, Weake et al., 2008)

Expression Deduced from Reporters

Reporter: P{not^T:Hsap\MYC}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic/larval optic lobe | restricted

• cytoplasm

(Poeck et al., 2001)

embryonic/larval brain | restricted

(Poeck et al., 2001)

lamina anlage | restricted

(Poeck et al., 2001)

adult lamina marginal glial cell

(Poeck et al., 2001)

epithelial glial cell

(Poeck et al., 2001)

adult medulla neuropil associated glial cell

(Poeck et al., 2001)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\not in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\not

View exonic expression by tissue for Dmel\not

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0013717

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40030/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013717

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013717

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013717

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4166

Flygut - An atlas of the Drosophila adult midgut

FBgn0013717

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 21 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of not

Transgenic constructs containing regulatory region of not

Aberrations (Deficiencies and Duplications) ( 14 )

Inferred from experimentation ( 14 )

Gene disrupted in

(Christensen et al., 2008.4.15)

(Christensen et al., 2009.5.6)

(Addison et al., 1995, Martin et al., 1995)

(Ryder, 2004.4.26)

Df(3L)GR844^X22

(Addison et al., 1995)

(Spradling et al., 1999, Addison et al., 1995, Martin et al., 1995, BDGP Project Members, 1994-1999)

(Martin et al., 1995, BDGP Project Members, 1994-1999)

Gene not disrupted in

(Addison et al., 1995)

(Addison et al., 1995)

(Addison et al., 1995)

(Addison et al., 1995)

(Addison et al., 1995)

Df(3L)GR844^X31

(Addison et al., 1995)

Df(3L)GR844^X33

(Addison et al., 1995)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

not^GD11236

lethal | heat sensitive, with Scer\GAL4^dpp.PU

not^GD11236

lethal | pupal stage, with Scer\GAL4^pnr-MD237

not^GD11236

lethal | recessive

lethal - all die before end of pupal stage | recessive

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

not^GD11236

Other Phenotypes

Allele

abnormal cell migration | oogenesis | oogenesis, with Scer\GAL4^c306, Spyo\Cas9^UAS.P2

not^WKO.P5-C6

abnormal cell migration | oogenesis | oogenesis, with Scer\GAL4^slbo.2.6

not^GD11236

abnormal cell migration | oogenesis | oogenesis, with Scer\GAL4^slbo.2.6, Spyo\Cas9^UAS.P2

not^WKO.P5-C6

abnormal cell migration | oogenesis | oogenesis | somatic clone

not¹

abnormal cell polarity | oogenesis | somatic clone

not¹

abnormal locomotor rhythm | adult stage, with Scer\GAL4^Pdf.PU

not^NIG.4166R

abnormal neuroanatomy

not⁰²⁰⁶⁹

abnormal neuroanatomy | somatic clone | third instar larval stage

not^F1935

abnormal neurophysiology | larval stage, with Scer\GAL4^egr-GAL4

not^JF03152

abnormal planar polarity | recessive | somatic clone

not¹

abnormal planar polarity | somatic clone

not¹

enhancer of variegation | dominant

not⁰²⁰⁶⁹

increased cell number | oogenesis | somatic clone

not¹

some die during prepupal stage | recessive (with Df(3L)W4)

not¹

some die during P-stage (with Df(3L)W4)

not¹

some die during pupal stage | recessive

suppressor of variegation

visible, with Scer\GAL4^bbg-C96

not^GD11236

visible, with Scer\GAL4^dpp.PU

not^GD11236

visible | adult stage, with Scer\GAL4^ey.PH

not^JF03152

visible | adult stage, with Scer\GAL4^nub.PK

not^JF03152

visible | somatic clone

not¹

Phenotype manifest in

Allele

adult lamina marginal glial cell

not^unspecified

adult lamina marginal glial cell | somatic clone

not¹

adult medulla neuropil associated glial cell

not^unspecified

axon & photoreceptor cell R2

not⁰²⁰⁶⁹

axon & photoreceptor cell R3

not⁰²⁰⁶⁹

axon & photoreceptor cell R4

not⁰²⁰⁶⁹

axon & photoreceptor cell R5

not⁰²⁰⁶⁹

border follicle cell, with Scer\GAL4^c306, Spyo\Cas9^UAS.P2

not^WKO.P5-C6

border follicle cell, with Scer\GAL4^slbo.2.6

not^GD11236

border follicle cell, with Scer\GAL4^slbo.2.6, Spyo\Cas9^UAS.P2

not^WKO.P5-C6

border follicle cell | increased number | somatic clone, with Scer\GAL4^slbo.2.6

not^GD11236

border follicle cell | somatic clone

not¹

cell projection | oogenesis

not¹

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^egr-GAL4

not^JF03152

epithelial glial cell

not^unspecified

epithelial glial cell | somatic clone

not¹

eye, with Scer\GAL4^ey.PH

not^JF03152

eye photoreceptor cell | somatic clone

not¹

not^unspecified

lamina anlage | precursor

not^unspecified

larval inner optic anlage | precursor

larval outer optic anlage | precursor

not^unspecified

ommatidium | somatic clone

not¹

photoreceptor cell & axon

not^unspecified

photoreceptor cell & axon (with Df(3L)W4)

not¹

photoreceptor cell R1 & axon

photoreceptor cell R2 & axon

photoreceptor cell R3 & axon

photoreceptor cell R4 & axon

photoreceptor cell R5 & axon

photoreceptor cell R6 & axon

polar follicle cell | increased number | somatic clone, with Scer\GAL4^slbo.2.6

not^GD11236

scutellar bristle | increased number, with Scer\GAL4^dpp.PU

not^GD11236

wing, with Scer\GAL4^bbg-C96

not^GD11236

wing, with Scer\GAL4^nub.PK

not^JF03152

wing hair | somatic clone

not¹

wing margin, with Scer\GAL4^nub.PK

not^JF03152

wing margin bristle, with Scer\GAL4^bbg-C96

not^GD11236

wing vein, with Scer\GAL4^nub.PK

not^JF03152

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

15 of 15

Yes

Yes

10 of 15

No

Yes

9 of 15

No

Yes

3 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1

1 of 15

No

No

1 of 15

No

No

3

1 of 15

No

No

2

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

15 of 15

Yes

Yes

9 of 15

No

Yes

9 of 15

No

Yes

3 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

Rattus norvegicus (Norway rat) (8)

13 of 13

Yes

Yes

9 of 13

No

Yes

3 of 13

No

Yes

2 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (8)

9 of 12

Yes

Yes

2 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

Yes

1 of 12

No

Yes

Danio rerio (Zebrafish) (12)

10 of 15

Yes

Yes

2 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (8)

4 of 15

Yes

Yes

2 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (1)

8 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

12 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Schizosaccharomyces pombe (Fission yeast) (5)

10 of 12

Yes

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (18)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

USP27X; ubiquitin specific peptidase 27 X-linked

model of non-syndromic X-linked intellectual disability 105

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

USP22; ubiquitin specific peptidase 22

15 of 15

USP27X; ubiquitin specific peptidase 27 X-linked

10 of 15

INTELLECTUAL DEVELOPMENTAL DISORDER, X-LINKED 105; XLID105

non-syndromic X-linked intellectual disability 105

USP51; ubiquitin specific peptidase 51

9 of 15

USP3; ubiquitin specific peptidase 3

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2009)

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Cloud et al., 2019, Weake et al., 2011, Weake et al., 2009)

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Cloud et al., 2019, Mohan et al., 2014)

anti bait coimmunoprecipitation, western blot

(Popova et al., 2018)

anti tag coimmunoprecipitation, molecular sieving, Identification by mass spectrometry, pull down, western blot, anti bait coimmunoprecipitation, two hybrid

(Erez et al., 2021)

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot

(Mohan et al., 2014, Gurskiy et al., 2012, Weake et al., 2008)

anti bait coimmunoprecipitation, western blot

(Popova et al., 2018)

tandem affinity purification, molecular sieving, multidimensional protein identification technology, anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation

(Cloud et al., 2019)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2009)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Stegeman et al., 2016)

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, molecular sieving, Identification by mass spectrometry, anti tag western blot, three hybrid

(Erez et al., 2021, Popova et al., 2018, Mohan et al., 2014, Gurskiy et al., 2012, Weake et al., 2008)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2009)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Stegeman et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Stegeman et al., 2016, Weake et al., 2008)

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, molecular sieving, Identification by mass spectrometry, three hybrid, pull down

(Erez et al., 2021, Popova et al., 2018, Gurskiy et al., 2012)

not - mod(mdg4)

anti tag coimmunoprecipitation, molecular sieving, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot, transcriptional complementation assay

(Erez et al., 2021)

(Battersby et al., 2003)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2009)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Badmos et al., 2021)

enhanceable

(Dietz et al., 2015)

enhanceable

(Poeck et al., 2001)

suppressible

(Badmos et al., 2021)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Wilk et al., 2004)

External Data

Subunit Structure (UniProtKB)

Component of the SAGA transcription coactivator-HAT complex, at least composed of Ada2b, not/nonstop, Pcaf/Gcn5, Sgf11 and Spt3.

(UniProt, Q9VVR1 )

Linkouts

BioGRID - A database of protein and genetic interactions.

65311

DroID - A comprehensive database of gene and protein interactions.

FBgn0013717

MIST (protein-protein) - An integrated Molecular Interaction Database

not

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0013717

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Ub-specific processing proteases

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3L

Recombination map

3-45

Cytogenetic map

Sequence location

3L:18,618,305..18,622,129 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

75D4-75D4

Limits computationally determined from genome sequence between P{PZ}W⁰⁵⁰¹⁴ and P{lacW}l(3)j14E7^j14E7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

75C1-75C2

(Poeck et al., 2001)

75C1-75C4

(Poeck, 1999.8.23)

75C3-75C4

(determined by in situ hybridisation)

(Spradling et al., 1999)

75C1-75C4

(determined by in situ hybridisation)

(Martin et al., 1995)

63A3-63A4

75C1--2

(BDGP Project Members, 1994-1999)

Mapped by recombination analysis and deficiency complementation tests (details unspecified).

(Poeck et al., 2001)

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-41.8

(Comeron et al., 2012)

3-

(Martin et al., 1995)

Left of (cM)

Right of (cM)

st

(Martin et al., 1995)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

84220

y¹ v¹; M{WKO.P5-C6}ZH-86Fb

19235

w¹¹¹⁸; P{XP}not^d06314/TM6B, Tb¹

28725

y¹ v¹; P{TRiP.JF03152}attP2/TM3, Sb¹

11553

P{PZ}not⁰²⁰⁶⁹ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

91284

y¹ w^*; TI{CRIMIC.TG4.0}not^{CR01723-TG4.0}/TM3, Sb¹ Ser¹

123668

w¹¹¹⁸; P{RS3}not^CB-5509-3

124296

w¹¹¹⁸; P{RS3}not^UM-8035-3

125130

w¹¹¹⁸; P{RS5}not^5-HA-1189

114066

w^*; P{GawB}not^NP7076 / TM6, P{UAS-lacZ.UW23-1}UW23-1

105235

y^* w^*; P{GawB}not^NP6511 / TM6, P{UAS-lacZ.UW23-1}UW23-1

v45775

w¹¹¹⁸; P{GD11236}v45775

v45776

w¹¹¹⁸; P{GD11236}v45776

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (27)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD43147

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0013717

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0013717

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40030

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Li et al., 2017, Gurskiy et al., 2012)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: not l(3)02069

(Salecker, 2001.3.5)

Source for merge of: not anon-WO0257455.9

(FlyBase, 1996-)

Additional comments

Source for merge of not anon-WO0257455.9 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Area matching Drosophila EST AA264796 (inverted).

(Davies, 2001.3.15)

The phenotype of not mutants suggests that not is required for the migration of the epithelial, marginal and medulla glial cells in the developing optic lobe.

(Poeck et al., 2001)

Genetic mosaic analysis fails to reveal a retinal requirement for not. Phenotypic analysis suggests not is not required in the optic ganglia to specify the correct termination site of R1-R6 neurons.

(Martin et al., 1995)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40030

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VVR1

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VVR1

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VVR1

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0013717

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0013717

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40030/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013717

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

not_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013717

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013717

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4166

Flygut - An atlas of the Drosophila adult midgut

FBgn0013717

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0013717

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40030

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006701

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4166

MARRVEL_MODEL - MARRVEL (model organism gene)

40030

modMine - A data warehouse for the modENCODE project

FBgn0013717

Linkouts

BioGRID - A database of protein and genetic interactions.

65311

DroID - A comprehensive database of gene and protein interactions.

FBgn0013717

DRSC - Results frm RNAi screens

FBgn0013717

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0013717

FlyMine - An integrated database for Drosophila genomics

FBgn0013717

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

not

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Ub-specific processing proteases

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

CG4166

(Brewer-Jensen et al., 2016, Stegeman et al., 2016, Zhou et al., 2015, Engel et al., 2014, Mohan et al., 2014, Tsou et al., 2012, Zhang et al., 2012, Ardehali et al., 2009, Perkins et al., 2009, Berger et al., 2008, Battersby et al., 2003)

NOT

(Barckmann and Simonelig, 2013, Xia et al., 2012)

Non-stop

(Cornelio-Parra et al., 2021)

Nonstop

(Popova et al., 2018, Spedale et al., 2012, Rodriguez-Navarro, 2009)

Not

(Erez et al., 2021, Torres-Zelada and Weake, 2021, Cacchione et al., 2020, Zhou et al., 2015, Engel et al., 2014, Zhang et al., 2012)

Q9VVR1

(Clague et al., 2013)

UBP8

(Weake et al., 2008)

USP22

(Weake et al., 2008)

Upb8

(Ardehali et al., 2009)

anon-WO0257455.9

dNonStop

(Spedale et al., 2012)

dNonstop

(Helmlinger et al., 2021)

i155

(Battersby et al., 2003)

l(3)02069

(Beaton, 1999.12.12, Spradling et al., 1999, Addison et al., 1995, BDGP Project Members, 1994-1999)

l(3)j9A6

non-stop

(Kim et al., 2020, Cloud et al., 2019)

nonstop

(Martin et al., 1995)

Name Synonyms

Non-stop

(Erez et al., 2021, Mohan et al., 2014)

Nonstop

(Gurskiy et al., 2012, Weake et al., 2011, Weake et al., 2009)

non-stop

(Li et al., 2017, Berger et al., 2008, Garcia et al., 2007)

nonstop

(Stegeman et al., 2016, Hadjieconomou et al., 2011, Hoopfer et al., 2008, Weake et al., 2008, Zhao et al., 2008, Kaplow et al., 2007, Chotard and Salecker, 2004, Pielage and Klambt, 2001)

Secondary FlyBase IDs

FBgn0010766
FBgn0011415
FBgn0063787

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (111)