FB2019_04, released Aug 22, 2019

Gene: Dmel\not

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General Information
Symbol
Dmel\not
Species
D. melanogaster
Name
non-stop
Annotation Symbol
CG4166
Feature Type
protein_coding_gene
FlyBase ID
FBgn0013717
Gene Model Status
Current
Stock Availability
13 publicly available
Enzyme Name (EC)
Ubiquitinyl hydrolase 1 (3.4.19.12)
Gene Snapshot
non-stop (not) encodes a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) chromatin modifying complex. It deubiquitinates the product of His2B at active promoters, thereby promoting transcription elongation. It was first identified for its role in neural development in the optic lobe. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As
l(3)02069
Key Links
Genomic Location
Cytogenetic map
75D4-75D4
Sequence location
3L:18,618,305..18,622,129 [-]
Recombination map
3-45
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the peptidase C19 family. UBP8 subfamily. (Q9VVR1)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
protein binding
Predictions / Assertions
thiol-dependent ubiquitin-specific protease activity; thiol-dependent ubiquitinyl hydrolase activity; zinc ion binding
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) (3.4.19.12)
Summaries
Gene Group (FlyBase)
UBIQUITIN SPECIFIC PROTEASES (DEUBIQUITINASES) -
The Ubiquitin Specific Proteases (USP) is the largest sub-family of deubiquitinases. They are cysteine proteases and catalyze the removal of ubiquitin from ubiquitin chains and ubiquitinated proteins. (Adapted from PMID:19626045).
SPT-ADA-GCN5 ACETYLTRANSFERASE -
The Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex is a chromatin modifying complex. SAGA can acetylate and deubiquitinate histone residues. Also SAGA has been shown to regulate the transcriptional activation of a subset of genes. (Adapted from FBrf0228923, FBrf0219841 and FBrf0233349).
Protein Function (UniProtKB)
Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of histone H2B, thereby acting as a coactivator in a large subset of genes. Required to counteract heterochromatin silencing. Controls the development of neuronal connectivity in visual system by being required for accurate axon targeting in the optic lobe. Required for expression of ecdysone-induced genes such as br/broad.
(UniProt, Q9VVR1)
Summary (Interactive Fly)
ubiquitin-specific protease involved in glial development - effects axon guidance
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\not or the JBrowse view of Dmel\not for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.44
Gene model reviewed during 5.56
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
not-RB
FBtr0290259
NM_079416
2488
496
not-RC
FBtr0346438
NM_001300177
2989
496
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
not-PB
FBpp0288698
56.4
496
8.03
NP_524140
AAF49249
not-PC
FBpp0312097
56.4
496
8.03
NP_001287106
AHN58131
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

496 aa isoforms: not-PB, not-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Component of the SAGA transcription coactivator-HAT complex, at least composed of Ada2b, not/nonstop, Pcaf/Gcn5, Sgf11 and Spt3.
(UniProt, Q9VVR1)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\not using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (17 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
protein binding
inferred from physical interaction with FLYBASE:Sgf11; FB:FBgn0036804
(Weake et al., 2008)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
thiol-dependent ubiquitin-specific protease activity
inferred from sequence model
(Poeck et al., 2001)
thiol-dependent ubiquitinyl hydrolase activity
inferred from electronic annotation with InterPro:IPR001394, InterPro:IPR018200
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
zinc ion binding
inferred from electronic annotation with InterPro:IPR001607
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (10 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
axon guidance
inferred from mutant phenotype
(Berger et al., 2008)
axon target recognition
inferred from mutant phenotype
(Martin et al., 1995)
chromatin organization
inferred from mutant phenotype
(Zhao et al., 2008)
glial cell development
inferred from mutant phenotype
(Poeck et al., 2001)
glial cell migration
inferred from mutant phenotype
(Poeck et al., 2001)
histone deubiquitination
inferred from mutant phenotype
(Weake et al., 2008)
inferred from direct assay
(Mohan et al., 2014)
positive regulation of transcription, DNA-templated
inferred from mutant phenotype
(assigned by UniProt )
(Zhao et al., 2008)
protein deubiquitination
inferred from mutant phenotype
(Poeck et al., 2001)
regulation of transcription, DNA-templated
inferred from mutant phenotype
(Weake et al., 2008)
ubiquitin-dependent protein catabolic process
inferred from genetic interaction with FLYBASE:Prosβ6; FB:FBgn0002284
(Poeck et al., 2001)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
DUBm complex
inferred from direct assay
(Mohan et al., 2014)
nucleus
inferred from direct assay
(Weake et al., 2011)
SAGA complex
inferred from direct assay
(Weake et al., 2011, Weake et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval optic lobe | faint | restricted
(Poeck et al., 2001)
lamina anlage | faint | restricted
(Poeck et al., 2001)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
DUBm complex
inferred from direct assay
(Mohan et al., 2014)
nucleus
inferred from direct assay
(Weake et al., 2011)
SAGA complex
inferred from direct assay
(Weake et al., 2011, Weake et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\not in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 19 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of not
Transgenic constructs containing regulatory region of not
Deletions and Duplications ( 14 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4pnr-MD237
lethal | heat sensitive, with Scer\GAL4dpp.PU
lethal | pupal stage, with Scer\GAL4pnr-MD237
lethal | recessive
lethal - all die before end of pupal stage | recessive
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
Other Phenotypes
Allele
enhancer of variegation | dominant
neuroanatomy defective
neuroanatomy defective | somatic clone | third instar larval stage
planar polarity defective | recessive | somatic clone
planar polarity defective | somatic clone
some die during prepupal stage | recessive (with Df(3L)W4)
some die during P-stage (with Df(3L)W4)
some die during pupal stage | recessive
suppressor of variegation
visible, with Scer\GAL4bbg-C96
visible, with Scer\GAL4dpp.PU
visible | adult stage, with Scer\GAL4ey.PH
visible | adult stage, with Scer\GAL4nub.PK
visible | somatic clone
wild-type
Phenotype manifest in
Allele
adult marginal glial cell
adult marginal glial cell | somatic clone
axon & photoreceptor cell R2
axon & photoreceptor cell R3
axon & photoreceptor cell R4
axon & photoreceptor cell R5
epithelial glial cell
epithelial glial cell | somatic clone
eye, with Scer\GAL4ey.PH
eye photoreceptor cell | somatic clone
lamina
lamina anlage | precursor
lamina plexus
larval inner optic anlage | precursor
larval outer optic anlage | precursor
medulla
medulla neuropil glial cell
ommatidium | somatic clone
photoreceptor cell & axon
photoreceptor cell & axon (with Df(3L)W4)
photoreceptor cell R1 & axon
photoreceptor cell R2 & axon
photoreceptor cell R3 & axon
photoreceptor cell R4 & axon
photoreceptor cell R5 & axon
photoreceptor cell R6 & axon
scutellar bristle | supernumerary, with Scer\GAL4dpp.PU
wing, with Scer\GAL4bbg-C96
wing, with Scer\GAL4nub.PK
wing hair | somatic clone
wing margin, with Scer\GAL4nub.PK
wing margin bristle, with Scer\GAL4bbg-C96
wing vein, with Scer\GAL4nub.PK
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (12)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\USP22
15 of 15
Yes
Yes
Hsap\USP27X
10 of 15
No
Yes
Hsap\USP51
10 of 15
No
Yes
Hsap\USP3
2 of 15
No
Yes
Hsap\USP2
1 of 15
No
No
Hsap\USP4
1 of 15
No
No
Hsap\USP8
1 of 15
No
No
Hsap\USP11
1 of 15
No
No
Hsap\USP30
1 of 15
No
No
 
Hsap\USP32
1 of 15
No
No
Hsap\USP44
1 of 15
No
Yes
Hsap\USP49
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (13)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Usp22
14 of 15
Yes
Yes
Mmus\Usp27x
9 of 15
No
Yes
Mmus\Usp51
9 of 15
No
Yes
Mmus\Usp3
2 of 15
No
Yes
Mmus\Usp2
1 of 15
No
No
Mmus\Usp4
1 of 15
No
No
Mmus\Usp8
1 of 15
No
No
Mmus\Usp11
1 of 15
No
No
Mmus\Usp15
1 of 15
No
No
Mmus\Usp30
1 of 15
No
No
Mmus\Usp32
1 of 15
No
No
Mmus\Usp44
1 of 15
No
Yes
Mmus\Usp49
1 of 15
No
No
Rattus norvegicus (Norway rat) (6)
Rnor\Usp22
13 of 13
Yes
Yes
Rnor\Usp51
10 of 13
No
Yes
Rnor\Usp27x
2 of 13
No
Yes
Rnor\Usp3
1 of 13
No
Yes
Rnor\Usp30
1 of 13
No
No
Rnor\Usp49
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (6)
Xtro\usp22
8 of 12
Yes
Yes
Xtro\usp3
1 of 12
No
Yes
Xtro\usp15
1 of 12
No
No
Xtro\usp30
1 of 12
No
No
Xtro\usp44
1 of 12
No
Yes
Xtro\usp49
1 of 12
No
Yes
Danio rerio (Zebrafish) (10)
Drer\usp22
9 of 15
Yes
Yes
Drer\usp2a
1 of 15
No
No
Drer\usp3
1 of 15
No
Yes
Drer\usp4
1 of 15
No
No
Drer\usp8
1 of 15
No
No
Drer\usp11
1 of 15
No
No
Drer\usp30
1 of 15
No
No
Drer\usp32
1 of 15
No
No
Drer\usp44
1 of 15
No
Yes
Drer\usp49
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (8)
Cele\usp-3
3 of 15
Yes
Yes
Cele\usp-33
2 of 15
No
No
Cele\C34F6.9
1 of 15
No
Yes
Cele\cyk-3
1 of 15
No
No
Cele\F07A11.4
1 of 15
No
No
Cele\H34C03.2
1 of 15
No
No
Cele\usp-50
1 of 15
No
No
Cele\Y67D2.2
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\UBP22
8 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (4)
Scer\UBP8
15 of 15
Yes
Yes
Scer\UBP1
1 of 15
No
No
Scer\UBP5
1 of 15
No
No
Scer\UBP12
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (6)
Spom\ubp8
11 of 12
Yes
Yes
Spom\ubp1
1 of 12
No
No
Spom\ubp4
1 of 12
No
No
Spom\ubp11
1 of 12
No
Yes
Spom\ubp12
1 of 12
No
No
Spom\ubp16
1 of 12
No
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190642 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\not
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00011261
Drosophila simulans
Dsim\GD12336
Drosophila sechellia
Dsec\GM14931
Drosophila erecta
Dere\GG15734
Drosophila yakuba
Dyak\GE22066
Drosophila ananassae
Dana\GF10813
Drosophila pseudoobscura pseudoobscura
Dpse\GA18001
Drosophila persimilis
Dper\GL22694
Drosophila willistoni
Dwil\GK11483
Drosophila virilis
Dvir\GJ11383
Drosophila mojavensis
Dmoj\GI11709
Drosophila grimshawi
Dgri\GH18640
Drosophila grimshawi
Dgri\GH17103
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503GQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17023229
Glossina morsitans
Tsetse fly
Gmor\GMOY000177
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08424
Mayetiola destructor
Hessian fly
Mdes\Mdes013648
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL003517
Anopheles darlingi
American malaria mosquito
Adar\ADAC000886
Anopheles gambiae
Malaria mosquito
Agam\AGAP012119
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ003218
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03KI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA007073
Danaus plexippus
Monarch butterfly
Dple\DPOGS203238-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL005827
Apis florea
Little honeybee
Aflo\2424429988
Apis mellifera
Western honey bee
Amel\LOC411920
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP11179
Bombus terrestris
Buff-tailed bumblebee
Bter\15208910
Linepithema humile
Argentine ant
Lhum\LH18754
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002b55
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG008862
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08386
Tribolium castaneum
Red flour beetle
Tcas\TC006701
Pediculus humanus
Human body louse
Phum\PHUM074960
Rhodnius prolixus
Kissing bug
Rpro\RPRC009463
Cimex lectularius
Bed bug
Clec\CLEC007182
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001431
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03895
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03HB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR004561
Ixodes scapularis
Black-legged tick
Isca\ISCW003597
Stegodyphus mimosarum
African social velvet spider
Smim\KFM72681.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63203.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur16g00200
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_305599
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G09GI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Usp22
Ciona intestinalis
Vase tunicate
Cint\LOC100175972
Gallus gallus
Domestic chicken
Ggal\USP22
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (8)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Usp2
2 of 10
Usp8
2 of 10
Usp32
2 of 10
scny
1 of 10
Usp15-31
1 of 10
Usp20-33
1 of 10
Usp30
1 of 10
Usp39
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    USP22; ubiquitin specific peptidase 22
    15 of 15
    612116
        USP27X; ubiquitin specific peptidase 27 X-linked
        10 of 15
        300975
          USP51; ubiquitin specific peptidase 51
          10 of 15
              Functional Complementation Data
              Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
              Interactions
              Summary of Physical Interactions
              esyN Network Diagram
              Show neighbor-neighbor interactions:
              Select Layout:
              Legend:
              Protein
              RNA
              Selected Interactor(s)
              Interactions Browser
              View in Interactions Browser
              Please see the Physical Interaction reports below for full details
              protein-protein
              Physical Interaction
              Assay
              References
              not - Ada1-2
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Weake et al., 2009)
              not - Ada2b
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Weake et al., 2011, Weake et al., 2009)
              not - Atxn7
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Mohan et al., 2014)
              not - Brf
              anti bait coimmunoprecipitation, western blot
              (Popova et al., 2018)
              not - Gcn5
              anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot
              (Mohan et al., 2014, Gurskiy et al., 2012, Weake et al., 2008)
              not - Pbp45
              anti bait coimmunoprecipitation, western blot
              (Popova et al., 2018)
              not - Saf6
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Weake et al., 2009)
              not - Sf3b5
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Stegeman et al., 2016)
              not - Sgf11
              anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot
              (Popova et al., 2018, Mohan et al., 2014, Gurskiy et al., 2012, Weake et al., 2008)
              not - Sgf29
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Weake et al., 2009)
              not - Spt20
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Stegeman et al., 2016)
              not - Spt3
              anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot
              (Stegeman et al., 2016, Weake et al., 2008)
              not - e(y)2
              anti bait coimmunoprecipitation, western blot
              (Popova et al., 2018, Gurskiy et al., 2012)
              not - stumps
              two hybrid
              (Battersby et al., 2003)
              not - wda
              anti tag coimmunoprecipitation, peptide massfingerprinting
              (Weake et al., 2009)
              Summary of Genetic Interactions
              esyN Network Diagram
              esyN Network Key:
              Suppression
              Enhancement
              View in Interactions Browser
              Please look at the allele data for full details of the genetic interactions
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              not
              enhanceable
              htt
              (Dietz et al., 2015)
              not
              enhanceable
              Prosβ6
              (Poeck et al., 2001)
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              peb
              suppressible
              not
              (Wilk et al., 2004)
              External Data
              Subunit Structure (UniProtKB)
              Component of the SAGA transcription coactivator-HAT complex, at least composed of Ada2b, not/nonstop, Pcaf/Gcn5, Sgf11 and Spt3.
              (UniProt, Q9VVR1 )
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DroID - A comprehensive database of gene and protein interactions.
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Pathways
              Gene Group - Pathway Membership (FlyBase)
              External Data
              Linkouts
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Genomic Location and Detailed Mapping Data
              Chromosome (arm)
              3L
              Recombination map
              3-45
              Cytogenetic map
              75D4-75D4
              Sequence location
              3L:18,618,305..18,622,129 [-]
              FlyBase Computed Cytological Location
              Cytogenetic map
              Evidence for location
              75D4-75D4
              Limits computationally determined from genome sequence between P{PZ}W05014 and P{lacW}l(3)j14E7j14E7
              Experimentally Determined Cytological Location
              Cytogenetic map
              Notes
              References
              75C1-75C2
              (Poeck et al., 2001)
              75C1-75C4
              (Poeck, 1999.8.23)
              75C3-75C4
              (determined by in situ hybridisation)
              (Spradling et al., 1999)
              75C1-75C4
              (determined by in situ hybridisation)
              (Martin et al., 1995)
              63A3-63A4
              75C1--2
              (BDGP Project Members, 1994-1999)
              Mapped by recombination analysis and deficiency complementation tests (details unspecified).
              (Poeck et al., 2001)
              Experimentally Determined Recombination Data
              Location
              3-45
              (FlyBase, 2016)
              3-41.8
              (Comeron et al., 2012)
              3-
              (Martin et al., 1995)
              Left of (cM)
              Right of (cM)
              (Martin et al., 1995)
              Notes
              Stocks and Reagents
              Stocks (13)
              Genomic Clones (13)
               

              Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

              cDNA Clones (27)
               

              Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

              cDNA clones, fully sequences
              BDGP DGC clones
              Other clones
                Drosophila Genomics Resource Center cDNA clones

                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                Other clones
                RNAi and Array Information
                Linkouts
                DRSC - Results frm RNAi screens
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                Antibody Information
                Laboratory Generated Antibodies
                 
                polyclonal
                (Li et al., 2017, Gurskiy et al., 2012)
                Commercially Available Antibodies
                 
                Other Information
                Relationship to Other Genes
                Source for database identify of
                Source for database merge of
                Source for merge of: not l(3)02069
                (Salecker, 2001.3.5)
                Source for merge of: not anon-WO0257455.9
                (FlyBase, 1996-)
                Additional comments
                Source for merge of not anon-WO0257455.9 was sequence comparison ( date:051113 ).
                (FlyBase, 1996-)
                Other Comments
                Area matching Drosophila EST AA264796 (inverted).
                (Davies, 2001.3.15)
                The phenotype of not mutants suggests that not is required for the migration of the epithelial, marginal and medulla glial cells in the developing optic lobe.
                (Poeck et al., 2001)
                Genetic mosaic analysis fails to reveal a retinal requirement for not. Phenotypic analysis suggests not is not required in the optic ganglia to specify the correct termination site of R1-R6 neurons.
                (Martin et al., 1995)
                Origin and Etymology
                Discoverer
                Etymology
                Identification
                External Crossreferences and Linkouts ( 48 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                Other crossreferences
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                modMine - A data warehouse for the modENCODE project
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                FLIGHT - Cell culture data for RNAi and other high-throughput technologies
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                FlyMine - An integrated database for Drosophila genomics
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Synonyms and Secondary IDs (17)
                Reported As
                Symbol Synonym
                CG4166
                (Brewer-Jensen et al., 2016, Stegeman et al., 2016, Zhou et al., 2015, Engel et al., 2014, Mohan et al., 2014, Tsou et al., 2012, Zhang et al., 2012, Ardehali et al., 2009, Perkins et al., 2009, Berger et al., 2008, Battersby et al., 2003)
                NOT
                (Barckmann and Simonelig, 2013, Xia et al., 2012)
                Nonstop
                (Popova et al., 2018, Spedale et al., 2012, Rodriguez-Navarro, 2009)
                Not
                (Zhou et al., 2015, Engel et al., 2014, Zhang et al., 2012)
                Q9VVR1
                (Clague et al., 2013)
                UBP8
                (Weake et al., 2008)
                USP22
                (Weake et al., 2008)
                Upb8
                (Ardehali et al., 2009)
                anon-WO0257455.9
                dNonStop
                (Spedale et al., 2012)
                i155
                (Battersby et al., 2003)
                l(3)02069
                (Beaton, 1999.12.12, Spradling et al., 1999, Addison et al., 1995, BDGP Project Members, 1994-1999)
                l(3)j9A6
                nonstop
                (Martin et al., 1995)
                not
                (Meltzer et al., 2019, Ma et al., 2016, Stegeman et al., 2016, Dietz et al., 2015, Rao et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Tsou et al., 2012, Gan et al., 2010, Ardehali et al., 2009, Christensen et al., 2009.5.6, Berger et al., 2008, Christensen et al., 2008.4.15, Weake et al., 2008, Zhao et al., 2008, Garcia et al., 2007, Kaplow et al., 2007)
                Name Synonyms
                Non-stop
                (Mohan et al., 2014)
                Nonstop
                (Gurskiy et al., 2012, Weake et al., 2011, Weake et al., 2009)
                non-stop
                (Berger et al., 2008, Garcia et al., 2007)
                nonstop
                (Stegeman et al., 2016, Hadjieconomou et al., 2011, Hoopfer et al., 2008, Weake et al., 2008, Zhao et al., 2008, Kaplow et al., 2007, Chotard and Salecker, 2004, Pielage and Klambt, 2001)
                Secondary FlyBase IDs
                • FBgn0010766
                • FBgn0011415
                • FBgn0063787
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                References (91)