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Gene Dmel\not

General Information
Symbol	Dmel\not	Species	D. melanogaster
Name	non-stop	Annotation symbol	CG4166
Feature type	protein_coding_gene	FlyBase ID	FBgn0013717
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	l(3)02069, nonstop
Genomic Location
Chromosome (arm)	3L	Recombination map	3-
Cytogenetic map	75D4-75D4	Sequence location	3L:18,611,906..18,615,229 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene non-stop is referred to in FlyBase by the symbol Dmel\not (CG4166, FBgn0013717). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: protein deubiquitination; histone deubiquitination; chromatin organization; regulation of transcription, DNA-dependent; axon guidance; glial cell migration; glial cell development; axon target recognition; ubiquitin-dependent protein catabolic process. 31 alleles are reported. The phenotypes of these alleles are annotated with: medulla neuropil glial cell; epithelial glial cell; medulla; lamina; lamina plexus; marginal glial cell. It has one annotated transcript and one annotated polypeptide. Protein features are: Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2; Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2, conserved site; Zinc finger, C2H2-like; Zinc finger, RING/FYVE/PHD-type; Zinc finger, UBP-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval Malpighian tubules, larval fat body, larval trachea, adult female reproductive system. Comments on Affy2 ProbeSet: ProbeSet 1624354_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of not. Gene sequence location is 3L:18611906..18615229.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 75D4-75D4   Limits computationally determined from genome sequence between P{PZ}W05014 and P{lacW}l(3)j14E7j14E7
Experimentally Determined Cytological Location
Cytogenetic map Notes References 75C1-75C4     (Poeck, 1999.8.23) 75C3-75C4   (determined by in situ hybridisation)   (Spradling et al., 1999) 75C1-75C4   (determined by in situ hybridisation)   (Martin et al., 1995) 63A3-63A4   75C1--2   (BDGP Project Members, 1994-1999) 75C1-75C2   Mapped by recombination analysis and deficiency complementation tests (details unspecified).   (Poeck et al., 2001)
Experimentally Determined Recombination Data
Location	3- (Martin et al., 1995)
Left of (cM)
Right of (cM)	st (Martin et al., 1995)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\not for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 DGC clone LD43147 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) not-RB FBtr0290259  NM_079416   2488   496
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein not-PB   FBpp0288698   56.4   496   8.03     NP_524140   AAF49249
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2, conserved site (IPR018200) Zinc finger, C2H2-like (IPR015880) Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2 (IPR001394) Zinc finger, UBP-type (IPR001607) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264796   AA801739   AA820116   AA820601   AA941623   AA949411   AA950379   AA951156   AA951310   AA979347   AE014296 AAF49249   AF179590 AAD53181   AI257102   AI455697   AI457012   AI511668   AI514506   AI516125   AI516428   AI519131   AI519470   AI530964   AQ025249   AW943643   AX094109   AX482641   AY058707 AAL13936   BG638332   BI368120   BI577603   BI577660   BI578812   CK131810   CZ473991   CZ474702   CZ474703   EV600748   G00744
UniProtKB/Swiss-Prot	Q9VVR1
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval optic lobe | faint & restricted   (Poeck et al., 2001) lamina anlage | faint & restricted   (Poeck et al., 2001)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Weake et al., 2011) SAGA complex inferred from direct assay (Weake et al., 2008, Weake et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval Malpighian tubules, larval fat body, larval trachea, adult female reproductive system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    253.375 Larval Midgut    78.8 Larval Hindgut    96.1 Larval Malpighian Tubules    129.1 Larval Fat Body    174.5 Larval Salivary Gland    48.9 Larval Trachea    147.6 Larval Carcass    81.975 Adult Head    58.3 Adult Eye    55.25 Adult Brain    158.9 Adult Thoracic-Abdominal Ganglion    138.2 Adult Crop    84 Adult Midgut    54.1 Adult Hindgut    54.4 Adult Malpighian Tubules    40.2 Adult Fat Body    76.8 Adult Salivary Gland    40.2 Adult Heart    77.475 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    229.2 Adult Testis    66.3 Adult Male Accessory Gland    45.1 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    253.375 Larval Midgut    78.8 Larval Hindgut    96.1 Larval Malpighian Tubules    129.1 Larval Fat Body    174.5 Larval Salivary Gland    48.9 Larval Trachea    147.6 Larval Carcass    81.975 Adult Head    58.3 Adult Eye    55.25 Adult Brain    158.9 Adult Thoracic-Abdominal Ganglion    138.2 Adult Crop    84 Adult Midgut    54.1 Adult Hindgut    54.4 Adult Malpighian Tubules    40.2 Adult Fat Body    76.8 Adult Salivary Gland    40.2 Adult Heart    77.475 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    229.2 Adult Testis    66.3 Adult Male Accessory Gland    45.1 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    253.375 Larval Midgut    78.8 Larval Hindgut    96.1 Larval Malpighian Tubules    129.1 Larval Fat Body    174.5 Larval Salivary Gland    48.9 Larval Trachea    147.6 Larval Carcass    81.975 Adult Head    58.3 Adult Eye    55.25 Adult Brain    158.9 Adult Thoracic-Abdominal Ganglion    138.2 Adult Crop    84 Adult Midgut    54.1 Adult Hindgut    54.4 Adult Malpighian Tubules    40.2 Adult Fat Body    76.8 Adult Salivary Gland    40.2 Adult Heart    77.475 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    229.2 Adult Testis    66.3 Adult Male Accessory Gland    45.1 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    253.375 Larval Midgut    78.8 Larval Hindgut    96.1 Larval Malpighian Tubules    129.1 Larval Fat Body    174.5 Larval Salivary Gland    48.9 Larval Trachea    147.6 Larval Carcass    81.975 Adult Head    58.3 Adult Eye    55.25 Adult Brain    158.9 Adult Thoracic-Abdominal Ganglion    138.2 Adult Crop    84 Adult Midgut    54.1 Adult Hindgut    54.4 Adult Malpighian Tubules    40.2 Adult Fat Body    76.8 Adult Salivary Gland    40.2 Adult Heart    77.475 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    229.2 Adult Testis    66.3 Adult Male Accessory Gland    45.1 Adult Carcass    49.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    253.375 Larval Midgut    78.8 Larval Hindgut    96.1 Larval Malpighian Tubules    129.1 Larval Fat Body    174.5 Larval Salivary Gland    48.9 Larval Trachea    147.6 Larval Carcass    81.975 Adult Head    58.3 Adult Eye    55.25 Adult Brain    158.9 Adult Thoracic-Abdominal Ganglion    138.2 Adult Crop    84 Adult Midgut    54.1 Adult Hindgut    54.4 Adult Malpighian Tubules    40.2 Adult Fat Body    76.8 Adult Salivary Gland    40.2 Adult Heart    77.475 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    229.2 Adult Testis    66.3 Adult Male Accessory Gland    45.1 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    253.375 Larval Midgut    78.8 Larval Hindgut    96.1 Larval Malpighian Tubules    129.1 Larval Fat Body    174.5 Larval Salivary Gland    48.9 Larval Trachea    147.6 Larval Carcass    81.975 Adult Head    58.3 Adult Eye    55.25 Adult Brain    158.9 Adult Thoracic-Abdominal Ganglion    138.2 Adult Crop    84 Adult Midgut    54.1 Adult Hindgut    54.4 Adult Malpighian Tubules    40.2 Adult Fat Body    76.8 Adult Salivary Gland    40.2 Adult Heart    77.475 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    229.2 Adult Testis    66.3 Adult Male Accessory Gland    45.1 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    253.375 Larval Midgut    78.8 Larval Hindgut    96.1 Larval Malpighian Tubules    129.1 Larval Fat Body    174.5 Larval Salivary Gland    48.9 Larval Trachea    147.6 Larval Carcass    81.975 Adult Head    58.3 Adult Eye    55.25 Adult Brain    158.9 Adult Thoracic-Abdominal Ganglion    138.2 Adult Crop    84 Adult Midgut    54.1 Adult Hindgut    54.4 Adult Malpighian Tubules    40.2 Adult Fat Body    76.8 Adult Salivary Gland    40.2 Adult Heart    77.475 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    229.2 Adult Testis    66.3 Adult Male Accessory Gland    45.1 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    253.375 Larval Midgut    78.8 Larval Hindgut    96.1 Larval Malpighian Tubules    129.1 Larval Fat Body    174.5 Larval Salivary Gland    48.9 Larval Trachea    147.6 Larval Carcass    81.975 Adult Head    58.3 Adult Eye    55.25 Adult Brain    158.9 Adult Thoracic-Abdominal Ganglion    138.2 Adult Crop    84 Adult Midgut    54.1 Adult Hindgut    54.4 Adult Malpighian Tubules    40.2 Adult Fat Body    76.8 Adult Salivary Gland    40.2 Adult Heart    77.475 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    229.2 Adult Testis    66.3 Adult Male Accessory Gland    45.1 Adult Carcass    49.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    58.3   NA  Eye    55.25   NA  Brain    158.9   253.375  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    138.2   NA  Crop    84   78.8  Midgut    54.1   96.1  Hindgut    54.4   129.1  Malpighian Tubules    40.2   174.5  Fat Body    76.8   48.9  Salivary Gland    40.2   NA  Heart    77.475   147.6  Trachea    NA   NA  VirginFemale Spermatheca    117.3   NA  InseminatedFemale Spermatheca    118.8   NA  Ovary    229.2   NA  Testis    66.3   NA  Male Accessory Gland    45.1   81.975  Carcass    49.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    21 central nervous system, pupae P8    14 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    4 head, mated 20-day male    4 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    22 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    21 central nervous system, pupae P8    14 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    4 head, mated 20-day male    4 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    22 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    21 central nervous system, pupae P8    14 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    4 head, mated 20-day male    4 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    22 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    21 central nervous system, pupae P8    14 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    4 head, mated 20-day male    4 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    22 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    21 central nervous system, pupae P8    14 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    4 head, mated 20-day male    4 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    22 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    21 central nervous system, pupae P8    14 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    4 head, mated 20-day male    4 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    22 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    21 central nervous system, pupae P8    14 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    4 head, mated 20-day male    4 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    22 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    21 central nervous system, pupae P8    14 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    4 head, mated 20-day male    4 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    16 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    22 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    63 embryo 04-06hr    34 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    24 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    9 embryo 18-20hr    6 embryo 20-22hr    6 embryo 22-24hr    6 larva L1    8 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    31 white prepupae new    31 white prepupae 12hr    21 white prepupae 24hr    26 pupae 2d postWPP    16 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    5 adult male 05day    8 adult male 30day    7 adult female 01day    13 adult female 05day    21 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (53) embryo 02-04hr  (63) embryo 04-06hr  (34) embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    24 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    9 embryo 18-20hr    6 embryo 20-22hr    6 embryo 22-24hr    6 larva L1    8 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9  (31) white prepupae new  (31) white prepupae 12hr    21 white prepupae 24hr  26 pupae 2d postWPP    16 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    5 adult male 05day    8 adult male 30day    7 adult female 01day    13 adult female 05day    21 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    63 embryo 04-06hr    34 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    24 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    9 embryo 18-20hr    6 embryo 20-22hr    6 embryo 22-24hr    6 larva L1    8 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    31 white prepupae new    31 white prepupae 12hr    21 white prepupae 24hr    26 pupae 2d postWPP    16 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    5 adult male 05day    8 adult male 30day    7 adult female 01day    13 adult female 05day    21 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    63 embryo 04-06hr    34 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    24 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    9 embryo 18-20hr    6 embryo 20-22hr    6 embryo 22-24hr    6 larva L1    8 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    31 white prepupae new    31 white prepupae 12hr    21 white prepupae 24hr    26 pupae 2d postWPP    16 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    5 adult male 05day    8 adult male 30day    7 adult female 01day    13 adult female 05day    21 adult female 30day    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    63 embryo 04-06hr    34 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    24 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    9 embryo 18-20hr    6 embryo 20-22hr    6 embryo 22-24hr    6 larva L1    8 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    31 white prepupae new    31 white prepupae 12hr    21 white prepupae 24hr    26 pupae 2d postWPP    16 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    5 adult male 05day    8 adult male 30day    7 adult female 01day    13 adult female 05day    21 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (53) embryo 02-04hr  (63) embryo 04-06hr  (34) embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    24 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    9 embryo 18-20hr    6 embryo 20-22hr    6 embryo 22-24hr    6 larva L1    8 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9  31 white prepupae new  31 white prepupae 12hr    21 white prepupae 24hr  26 pupae 2d postWPP    16 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    5 adult male 05day    8 adult male 30day    7 adult female 01day    13 adult female 05day    21 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    63 embryo 04-06hr    34 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    24 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    9 embryo 18-20hr    6 embryo 20-22hr    6 embryo 22-24hr    6 larva L1    8 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    31 white prepupae new    31 white prepupae 12hr    21 white prepupae 24hr    26 pupae 2d postWPP    16 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    5 adult male 05day    8 adult male 30day    7 adult female 01day    13 adult female 05day    21 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    63 embryo 04-06hr    34 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    24 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    9 embryo 18-20hr    6 embryo 20-22hr    6 embryo 22-24hr    6 larva L1    8 larva L2    8 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    31 white prepupae new    31 white prepupae 12hr    21 white prepupae 24hr    26 pupae 2d postWPP    16 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    5 adult male 05day    8 adult male 30day    7 adult female 01day    13 adult female 05day    21 adult female 30day    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    15 embryonic 1182-4H    7 embryonic GM2    14 embryonic Kc167    8 embryonic S1    9 embryonic S3    4 leg disc CME L1    11 wing disc CME-W2    5 wing disc ML-DmD8    6 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    12 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    3 ovary fGS/OSS    6 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    15 embryonic 1182-4H    7 embryonic GM2    14 embryonic Kc167    8 embryonic S1    9 embryonic S3    4 leg disc CME L1    11 wing disc CME-W2    5 wing disc ML-DmD8    6 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    12 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    3 ovary fGS/OSS    6 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    15 embryonic 1182-4H    7 embryonic GM2    14 embryonic Kc167    8 embryonic S1    9 embryonic S3    4 leg disc CME L1    11 wing disc CME-W2    5 wing disc ML-DmD8    6 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    12 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    3 ovary fGS/OSS    6 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    15 embryonic 1182-4H    7 embryonic GM2    14 embryonic Kc167    8 embryonic S1    9 embryonic S3    4 leg disc CME L1    11 wing disc CME-W2    5 wing disc ML-DmD8    6 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    12 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    3 ovary fGS/OSS    6 ovary OSC    12 ovary OSS    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    15 embryonic 1182-4H    7 embryonic GM2    14 embryonic Kc167    8 embryonic S1    9 embryonic S3    4 leg disc CME L1    11 wing disc CME-W2    5 wing disc ML-DmD8    6 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    12 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    3 ovary fGS/OSS    6 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    15 embryonic 1182-4H    7 embryonic GM2    14 embryonic Kc167    8 embryonic S1    9 embryonic S3    4 leg disc CME L1    11 wing disc CME-W2    5 wing disc ML-DmD8    6 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    12 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    3 ovary fGS/OSS    6 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    15 embryonic 1182-4H    7 embryonic GM2    14 embryonic Kc167    8 embryonic S1    9 embryonic S3    4 leg disc CME L1    11 wing disc CME-W2    5 wing disc ML-DmD8    6 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    12 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    3 ovary fGS/OSS    6 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    15 embryonic 1182-4H    7 embryonic GM2    14 embryonic Kc167    8 embryonic S1    9 embryonic S3    4 leg disc CME L1    11 wing disc CME-W2    5 wing disc ML-DmD8    6 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    12 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    12 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    3 ovary fGS/OSS    6 ovary OSC    12 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    12 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_23 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013717 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4166-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 notGD11236 lethal | heat sensitive, with Scer\GAL4dpp.PU notGD11236 lethal | partially | prepupal stage (with Df(3L)W4) not1 lethal | pupal stage, with Scer\GAL4pnr-MD237 notGD11236 lethal | pupal stage | recessive not1 not02069 notS008913 notS138410 lethal | recessive not02069 not06806 notj9A6 Other Phenotypes Allele enhancer of variegation | dominant not02069 neuroanatomy defective not02069 neuroanatomy defective | somatic clone | third instar larval stage notF1935 planar polarity defective | recessive | somatic clone not1 planar polarity defective | somatic clone not1 suppressor of variegation notGMR.PZ notUCH-2.GMR visible, with Scer\GAL4bbg-C96 notGD11236 visible, with Scer\GAL4dpp.PU notGD11236 visible | somatic clone not1 wild-type noths.PP notT:Hsap\MYC Phenotype manifest in Allele axon & photoreceptor cell R2 not02069 axon & photoreceptor cell R3 not02069 axon & photoreceptor cell R4 not02069 axon & photoreceptor cell R5 not02069 epithelial glial cell notunspecified epithelial glial cell | somatic clone not1 eye photoreceptor cell | somatic clone not1 lamina notunspecified lamina anlage | precursor not02069 not1 lamina plexus notunspecified larval inner optic anlage | precursor not02069 not1 larval outer optic anlage | precursor not02069 not1 marginal glial cell notunspecified marginal glial cell | somatic clone not1 medulla notunspecified medulla neuropil glial cell notunspecified ommatidium | somatic clone not1 photoreceptor cell & axon notunspecified photoreceptor cell & axon (with Df(3L)W4) not1 photoreceptor cell R1 & axon not02069 not1 photoreceptor cell R2 & axon not02069 not1 photoreceptor cell R3 & axon not02069 not1 photoreceptor cell R4 & axon not02069 not1 photoreceptor cell R5 & axon not02069 not1 photoreceptor cell R6 & axon not02069 not1 scutellar bristle | supernumerary, with Scer\GAL4dpp.PU notGD11236 wing, with Scer\GAL4bbg-C96 notGD11236 wing hair | somatic clone not1 wing margin bristle, with Scer\GAL4bbg-C96 notGD11236
Classical Alleles ( 19 )
For All Classical Alleles Show
Allele of not Class Mutagen Stocks Known lesion notd06314 P-element activity 2 -- not02069 amorphic allele - genetic evidence P-element activity 1 Yes not5-HA-1189 1 -- notCB-5509-3 1 -- notd04543 P-element activity 1 -- notd05745 P-element activity 1 -- notNP6511 1 -- notNP7076 1 -- notUM-8035-3 1 -- not1 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- not06806 P-element activity 0 -- notDG24306 0 -- notEP3066 P-element activity 0 -- notF1935 ethyl methanesulfonate 0 Yes notj4B8a P-element activity 0 -- notj9A6 P-element activity 0 -- notS008913 P-element activity 0 Yes notS138410 P-element activity 0 Yes notunspecified 0 --
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of not Class Mutagen Stocks Known lesion notGD11236 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes notJF03152 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes notGMR.PB in vitro construct - regulatory fusion 0 Yes notGMR.PZ in vitro construct - regulatory fusion 0 Yes noths.PP in vitro construct - regulatory fusion 0 Yes notMtnA.T:Zzzz\FLAG,T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes notNIG.4166R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes notScer\UAS.cPa in vitro construct - regulatory fusion 0 Yes notScer\UAS.cZa in vitro construct - regulatory fusion 0 Yes notT:Hsap\MYC in vitro construct - coding region fusion 0 Yes notUCH-2.GMR in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes notZnF-UBP.GMR in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)BSC416 (Christensen et al., 2008.4.15) Df(3L)BSC775 (Christensen et al., 2009.5.6) Df(3L)Cat (Martin et al., 1995, Addison et al., 1995) Df(3L)Exel6134 (Ryder, 2004.4.26) Df(3L)GR844X22 (Addison et al., 1995) Df(3L)W4 (Spradling et al., 1999, BDGP Project Members, 1994-1999, Martin et al., 1995, Addison et al., 1995) Df(3L)W10 (BDGP Project Members, 1994-1999, Martin et al., 1995)
Not disrupted in	Df(3L)GR844X31 (Addison et al., 1995) Df(3L)GR844X33 (Addison et al., 1995) Df(3L)Δ26 (Addison et al., 1995) Df(3L)Δ32 (Addison et al., 1995) Df(3L)Δ54 (Addison et al., 1995) Df(3L)Δ215 (Addison et al., 1995) Df(3L)Δ3002 (Addison et al., 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11236} NA P{NIG.4166R} NA P{TRiP.JF03152} NA P{UAS-not.P} NA P{UAS-not.Z} NA heat-shock construct P{hs-not.XE} NA characterization construct P{GMR-not.B} NA P{GMR-not.UCH-2} NA P{GMR-not.Z} NA P{GMR-not.ZnF-UBP} NA P{notT:Hsap\MYC} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}notEP3066 NA P{EPgy2}tRNA:CR32200EY03870 NA P{XP}notd04543 NA P{XP}notd05745 NA P{XP}notd06314 NA insertion of enhancer trap P{lacW}notj4B8a No P{lacW}notj9A6 No P{lacW}notS008913 No P{lacW}notS138410 No P{PZ}not02069 No P{PZ}not06806 No miscellaneous insertions P{RS3}notCB-5509-3 NA P{RS3}notUM-8035-3 NA P{RS5}not5-HA-1189 NA P{wHy}notDG24306 NA insertion of enhancer trap binary system P{GawB}notNP6511 No P{GawB}notNP7076 No
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Sgf11 (Weake et al., 2008)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Berger et al., 2008) axon target recognition inferred from mutant phenotype (Martin et al., 1995, Weake et al., 2008) chromatin organization inferred from mutant phenotype (Zhao et al., 2008) glial cell development inferred from mutant phenotype (Poeck et al., 2001) glial cell migration inferred from mutant phenotype (Poeck et al., 2001) histone deubiquitination inferred from mutant phenotype (Weake et al., 2008) protein deubiquitination inferred from mutant phenotype (Poeck et al., 2001) regulation of transcription, DNA-dependent inferred from mutant phenotype (Weake et al., 2008) ubiquitin-dependent protein catabolic process inferred from genetic interaction with Pros26 (Poeck et al., 2001)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Weake et al., 2011) SAGA complex inferred from direct assay (Weake et al., 2008, Weake et al., 2011)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
ubiquitin-specific protease activity inferred from sequence or structural similarity with Pfam:PF00443 (Poeck et al., 2001) ubiquitin thiolesterase activity inferred from electronic annotation with InterPro:IPR001394, InterPro:IPR018200 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR001607, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions not allele Gene References not1 Prosβ6 (Poeck et al., 2001) not02069 peb (Wilk et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65311 DroID - A comprehensive database of gene and protein interactions. • FBgn0013717 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40030
Orthologs
OrthoDB Orthologs (43) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG61VKNN Dipteran inclusive ortholog search EOG68PMTK Insect inclusive ortholog search EOG6C86NN Arthropod inclusive ortholog search EOG676HFG Metazoa inclusive ortholog search EOG6MD8SK
Orthologs in Drosophila Species (EOG61VKNN)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\not     Drosophila simulans   Dsim\GD12336 Y   Drosophila sechellia   Dsec\GM14931 Y   Drosophila erecta   Dere\GG15734 Y   Drosophila yakuba   Dyak\GE22066 Y   Drosophila ananassae   Dana\GF10813 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18001 Y   Drosophila persimilis   Dper\GL22694 Y   Drosophila willistoni   Dwil\GK11483 Y   Drosophila virilis   Dvir\GJ11383 Y   Drosophila mojavensis   Dmoj\GI11709 Y   Drosophila grimshawi   Dgri\GH18640 Y   Drosophila grimshawi   Dgri\GH17103
Orthologs in non-Drosophila Dipterans (EOG68PMTK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL003517   Anopheles gambiae Malaria mosquito  Agam\AGAP012119   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003218
Orthologs in non-Dipteran Insects (EOG6C86NN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC411920   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG008862   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH20654   Atta cephalotes Leafcutter ant  Acep\ACEP17179   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22414   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16940   Linepithema humile Argentine ant  Lhum\LH18754   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17003   Solenopsis invicta Red fire ant  Sinv\SINV12172   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001431   Bombyx mori Silkmoth  Bmor\BGIBMGA007073   Pediculus humanus Human body louse  Phum\PHUM074960   Tribolium castaneum Red flour beetle  Tcas\TC006701
Orthologs in non-Insect Arthropods (EOG676HFG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_305599   Ixodes scapularis Deer tick  Isca\ISCW003597
Orthologs in non-Arthropod Metazoa (EOG6MD8SK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011940gn   Danio rerio Zebrafish  Drer\usp22   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000006051   Gallus gallus Domestic chicken  Ggal\USP22   Mus musculus House mouse  Mmus\Usp51   Mus musculus House mouse  Mmus\Usp27   Rattus norvegicus Norway rat  Rnor\Usp27x-ps1   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029228   Rattus norvegicus Norway rat  Rnor\Usp22   Rattus norvegicus Norway rat  Rnor\Usp51   Homo sapiens Human  Hsap\ENSG00000124422   Homo sapiens Human  Hsap\USP51
Human Orthologs (2)
Gene OMIM HGNC Hsap\ENSG00000124422     Hsap\USP51
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12336 Drosophila sechellia Dsec\GM14931 Drosophila erecta Dere\GG15734 Drosophila yakuba Dyak\GE22066 Drosophila ananassae Dana\GF10813 Drosophila pseudoobscura pseudoobscura Dpse\GA18001 Drosophila persimilis Dper\GL22694 Drosophila willistoni Dwil\GK11483 Drosophila virilis Dvir\GJ11383 Drosophila mojavensis Dmoj\GI11709 Drosophila grimshawi Dgri\GH18640
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	19235 w1118; P{XP}notd06314/TM6B, Tb1 28725 y1 v1; P{TRiP.JF03152}attP2/TM3, Sb1 11553 P{PZ}not02069 ry506/TM6B, ryCB Tb+
Harvard	- P{XP}notd04543 - P{XP}notd05745 - P{XP}notd06314
Kyoto	123668 w1118; P{RS3}notCB-5509-3 124296 w1118; P{RS3}notUM-8035-3 125130 w1118; P{RS5}not5-HA-1189 114066 w*; P{GawB}notNP7076 / TM6, P{UAS-lacZ.UW23-1}UW23-1
VDRC	v45775 w1118; P{GD11236}v45775 v45776 w1118; P{GD11236}v45776
	More stocks available...
Genomic Clones ( 2 )
	BACR17L05 BACR23M20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 26 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD43147
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM03429 LD08565 LD24415 LD25860 LD28303 LD28916 LD31476 LD31714 LD33858 LD35340 LD37217 LD38564 LD39076 LD41034 LD41882 LD42290 LP05370 LP12826 RE54037 RE71371 RE72741 SD01775
Other clones	Dmel_neb_008896_0868_0325 LD22730 RE71311
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013717 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40030
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: not anon-WO0257455.9 (FlyBase, 1996-) Source for merge of: not l(3)02069 (Salecker, 2001.3.5)
Additional comments	Source for merge of not anon-WO0257455.9 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	Area matching Drosophila EST AA264796 (inverted). (Davies, 2001.3.15) The phenotype of not mutants suggests that not is required for the migration of the epithelial, marginal and medulla glial cells in the developing optic lobe. (Poeck et al., 2001) Genetic mosaic analysis fails to reveal a retinal requirement for not. Phenotypic analysis suggests not is not required in the optic ganglia to specify the correct termination site of R1-R6 neurons. (Martin et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079416 RefSeq (Proteins) • NP_524140 DDBJ / EMBL / GenBank • AA264796 AAD53181 AAF49249 AAL13936 AF179590 AI511668 AQ025249 AW943643 AX094109 AX482641 AY058707 CZ473991 CZ474702 CZ474703 G00744 Entrez Gene - A searchable database of RefSeq genes. • 40030 UniProtKB/Swiss-Prot • Q9VVR1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2, conserved site (IPR018200) Zinc finger, C2H2-like (IPR015880) Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2 (IPR001394) Zinc finger, UBP-type (IPR001607) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Linkouts
	BioGRID - A database of protein and genetic interactions • 65311 DroID - A comprehensive database of gene and protein interactions. • FBgn0013717 DRSC - Results from RNAi screens. • FBgn0013717 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013717 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4166-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013717 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40030 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/nonstp1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40030 modMine - Data generated by the modENCODE project. • FBgn0013717
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	anon-WO0257455.9   CG4166 (Battersby et al., 2003, Ardehali et al., 2009, Zhang et al., 2012, Perkins et al., 2009, Berger et al., 2008, Tsou et al., 2012) i155 (Battersby et al., 2003) l(3)02069 (Beaton, 1999.12.12, Spradling et al., 1999, Addison et al., 1995, BDGP Project Members, 1994-1999, ) l(3)j9A6   nonstop (Martin et al., 1995) Nonstop (Spedale et al., 2012) not (Kaplow et al., 2007, Garcia et al., 2007, Gan et al., 2010, Christensen et al., 2008.4.15, Weake et al., 2008, Zhao et al., 2008, Ardehali et al., 2009, Christensen et al., 2009.5.6, Berger et al., 2008, Tsou et al., 2012, Japanese National Institute of Genetics, 2012.5.21) NOT (Xia et al., 2012) Not (Zhang et al., 2012) UBP8 (Weake et al., 2008) Upb8 (Ardehali et al., 2009) USP22 (Weake et al., 2008)
Name Synonym	nonstop (Chotard and Salecker, 2004, Pielage and Klambt, 2001, Hoopfer et al., 2008, Weake et al., 2008, Zhao et al., 2008, Kaplow et al., 2007) non-stop (Garcia et al., 2007, Berger et al., 2008) Nonstop (Weake et al., 2011, Weake et al., 2009)
Secondary FlyBase IDs
	FBgn0010766 FBgn0011415 FBgn0063787
References ( 62 )
Generate a list of	All references relating to this gene ( 62 )
List References by type	Research paper  ( 20 ) Supplementary material  ( 4 ) Review  ( 5 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 7 ) FlyBase analysis  ( 3 ) Conference report  ( 1 ) Stock list  ( 1 ) Patent  ( 2 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Tsou et al., 2012, PLoS ONE 7(8): e43112 Systematic Analysis of the Physiological Importance of Deubiquitinating Enzymes. [FBrf0219256] Xia et al., 2012, PLoS Biol. 10(1): e1001238 USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. [FBrf0218247] Weake et al., 2011, Genes Dev. 25(14): 1499--1509 Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. [FBrf0214372] Show all research papers ...
Recent reviews ( 1 )
	Spedale et al., 2012, Genes Dev. 26(6): 527--541 ATAC-king the complexity of SAGA during evolution. [FBrf0219841] Show all reviews ...