FB2025_02 , released April 17, 2025
Gene: Dmel\not
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General Information
Symbol
Dmel\not
Species
D. melanogaster
Name
non-stop
Annotation Symbol
CG4166
Feature Type
protein_coding_gene
FlyBase ID
FBgn0013717
Gene Model Status
Current
Stock Availability
14 publicly available
Enzyme Name (EC)
ubiquitinyl hydrolase 1 (3.4.19.12)
Gene Summary
non-stop (not) encodes a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) chromatin modifying complex. It deubiquitinates the product of His2B at active promoters, thereby promoting transcription elongation. It was first identified for its role in neural development in the optic lobe. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

l(3)02069

Key Links
Genomic Location
Cytogenetic map
75D4-75D4
Sequence location
Recombination map
3-45
RefSeq locus
NT_037436 REGION:18618305..18622129
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:Sgf11; FB:FBgn0036804
(Weake et al., 2008)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables cysteine-type deubiquitinase activity
inferred from sequence model
(Poeck et al., 2001)
inferred from electronic annotation with InterPro:IPR001394, InterPro:IPR018200
(InterPro Project Members, 2004-)
enables zinc ion binding
inferred from electronic annotation with InterPro:IPR001607
(InterPro Project Members, 2004-)
Biological Process (8 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
involved_in axon target recognition
inferred from mutant phenotype
(Martin et al., 1995)
involved_in chromatin organization
inferred from mutant phenotype
(Zhao et al., 2008)
involved_in glial cell development
inferred from mutant phenotype
(Poeck et al., 2001)
involved_in glial cell migration
inferred from mutant phenotype
(Poeck et al., 2001)
involved_in positive regulation of DNA-templated transcription
inferred from mutant phenotype
(Zhao et al., 2008)
involved_in regulation of DNA-templated transcription
inferred from mutant phenotype
(Weake et al., 2008)
involved_in ubiquitin-dependent protein catabolic process
inferred from genetic interaction with FLYBASE:Prosβ6; FB:FBgn0002284
(Poeck et al., 2001)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in protein deubiquitination
inferred from electronic annotation with InterPro:IPR001394
(InterPro Project Members, 2004-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
part_of DUBm complex
inferred from direct assay
(Mohan et al., 2014)
located_in nucleus
inferred from direct assay
(Weake et al., 2011)
part_of SAGA complex
inferred from direct assay
(Weake et al., 2011, Weake et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the peptidase C19 family. UBP8 subfamily. (Q9VVR1)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
cysteine-type deubiquitinase activity
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) (3.4.19.12)
Summaries
Gene Snapshot
non-stop (not) encodes a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) chromatin modifying complex. It deubiquitinates the product of His2B at active promoters, thereby promoting transcription elongation. It was first identified for its role in neural development in the optic lobe. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
USP DEUBIQUITINASES -
The Ubiquitin Specific Proteases (USP) is the largest sub-family of deubiquitinases. They are cysteine proteases and catalyze the removal of ubiquitin from ubiquitin chains and ubiquitinated proteins. USP deubiquitinases belong to MEROPS family C19. (Adapted from PMID:19626045).
SAGA COMPLEX -
The Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex is a chromatin modifying complex. SAGA can acetylate and deubiquitinate histone residues. Also SAGA has been shown to regulate the transcriptional activation of a subset of genes. (Adapted from FBrf0228923, FBrf0219841 and FBrf0233349).
Protein Function (UniProtKB)
Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of histone H2B, thereby acting as a coactivator in a large subset of genes. Required to counteract heterochromatin silencing. Controls the development of neuronal connectivity in visual system by being required for accurate axon targeting in the optic lobe. Required for expression of ecdysone-induced genes such as br/broad.
(UniProt, Q9VVR1)
Summary (Interactive Fly)

ubiquitin-specific protease involved in glial development - effects axon guidance

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\not for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VVR1)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.56

A non-AUG start codon may be used for translation of one or more transcripts of this gene; based on the presence of conserved protein signatures within the 5' UTR without an in-frame AUG (FBrf0243886).

Gene model reviewed during 5.44

Gene model reviewed during 6.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
not-RB
FBtr0290259
NM_079416
2488
496
not-RC
FBtr0346438
NM_001300177
2989
496
not-RD
FBtr0481736
NM_001414092
2488
703
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
not-PB
FBpp0288698
56.4
496
8.03
Q9VVR1
NP_524140
AAF49249
not-PC
FBpp0312097
56.4
496
8.03
Q9VVR1
NP_001287106
AHN58131
not-PD
FBpp0428493
78.7
703
8.86
UYI58710
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

496 aa isoforms: not-PB, not-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the SAGA transcription coactivator-HAT complex, at least composed of Ada2b, not/nonstop, Pcaf/Gcn5, Sgf11 and Spt3.

(UniProt, Q9VVR1)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\not using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.21

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval optic lobe | faint | restricted
(Poeck et al., 2001)
lamina anlage | faint | restricted
(Poeck et al., 2001)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of DUBm complex
inferred from direct assay
(Mohan et al., 2014)
located_in nucleus
inferred from direct assay
(Weake et al., 2011)
part_of SAGA complex
inferred from direct assay
(Weake et al., 2011, Weake et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\not in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 22 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of not
Transgenic constructs containing regulatory region of not
Aberrations (Deficiencies and Duplications) ( 14 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4pnr-MD237
lethal | heat sensitive, with Scer\GAL4dpp.PU
lethal | pupal stage, with Scer\GAL4pnr-MD237
lethal | recessive
lethal - all die before end of pupal stage | recessive
lethal - all die during larval stage
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
Other Phenotypes
Allele
abnormal cell migration | oogenesis, with Scer\GAL4c306, Spyo\Cas9UAS.P2
abnormal cell migration | oogenesis, with Scer\GAL4slbo.2.6
abnormal cell migration | oogenesis, with Scer\GAL4slbo.2.6, Spyo\Cas9UAS.P2
abnormal cell migration | oogenesis | somatic clone
abnormal cell polarity | oogenesis | somatic clone
abnormal locomotor rhythm | adult stage, with Scer\GAL4Pdf.PU
abnormal neuroanatomy
abnormal neuroanatomy | somatic clone | third instar larval stage
abnormal neurophysiology | larval stage, with Scer\GAL4egr-GAL4
abnormal planar polarity | recessive | somatic clone
abnormal planar polarity | somatic clone
enhancer of variegation | dominant
increased cell number | oogenesis | somatic clone
majority die during second instar larval stage
some die during prepupal stage | recessive (with Df(3L)W4)
some die during P-stage (with Df(3L)W4)
some die during pupal stage | recessive
some die during third instar larval stage
suppressor of variegation
visible, with Scer\GAL4bbg-C96
visible, with Scer\GAL4dpp.PU
visible | adult stage, with Scer\GAL4ey.PH
visible | adult stage, with Scer\GAL4nub.PK
visible | somatic clone
wild-type
Phenotype manifest in
Allele
adult lamina marginal glial cell
adult lamina marginal glial cell | somatic clone
adult medulla neuropil associated glial cell
axon & photoreceptor cell R2
axon & photoreceptor cell R3
axon & photoreceptor cell R4
axon & photoreceptor cell R5
border follicle cell, with Scer\GAL4c306, Spyo\Cas9UAS.P2
border follicle cell, with Scer\GAL4slbo.2.6
border follicle cell, with Scer\GAL4slbo.2.6, Spyo\Cas9UAS.P2
border follicle cell | increased number | somatic clone, with Scer\GAL4slbo.2.6
border follicle cell | somatic clone
cell projection | oogenesis
embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4egr-GAL4
epithelial glial cell
epithelial glial cell | somatic clone
eye, with Scer\GAL4ey.PH
eye photoreceptor cell | somatic clone
lamina
lamina anlage | precursor
lamina plexus
larval inner optic anlage | precursor
larval outer optic anlage | precursor
medulla
ommatidium | somatic clone
photoreceptor cell & axon
photoreceptor cell & axon (with Df(3L)W4)
photoreceptor cell R1 & axon
photoreceptor cell R2 & axon
photoreceptor cell R3 & axon
photoreceptor cell R4 & axon
photoreceptor cell R5 & axon
photoreceptor cell R6 & axon
polar follicle cell | increased number | somatic clone, with Scer\GAL4slbo.2.6
scutellar bristle | increased number, with Scer\GAL4dpp.PU
wing, with Scer\GAL4bbg-C96
wing, with Scer\GAL4nub.PK
wing hair | somatic clone
wing margin, with Scer\GAL4nub.PK
wing margin bristle, with Scer\GAL4bbg-C96
wing vein, with Scer\GAL4nub.PK
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (73)
Hsap\USP22
14 of 14
Yes
Yes
Hsap\USP27X
12 of 14
No
Yes
Hsap\USP51
12 of 14
No
Yes
Hsap\USP3
3 of 14
No
Yes
Hsap\USP30
2 of 14
No
No
3  
Hsap\USP36
2 of 14
No
No
Hsap\USP42
2 of 14
No
No
Hsap\USP44
2 of 14
No
Yes
Hsap\USP49
2 of 14
No
Yes
Hsap\USP1
1 of 14
No
No
2  
Hsap\USP2
1 of 14
No
No
Hsap\USP4
1 of 14
No
No
Hsap\USP5
1 of 14
No
No
Hsap\USP6
1 of 14
No
No
Hsap\USP7
1 of 14
No
No
2  
Hsap\USP8
1 of 14
No
No
1  
Hsap\USP9X
1 of 14
No
No
Hsap\USP9Y
1 of 14
No
No
Hsap\USP10
1 of 14
No
No
Hsap\USP11
1 of 14
No
No
Hsap\USP12
1 of 14
No
No
Hsap\USP13
1 of 14
No
No
1  
Hsap\USP14
1 of 14
No
No
Hsap\USP15
1 of 14
No
No
Hsap\USP16
1 of 14
No
No
Hsap\USP17L1
1 of 14
No
No
Hsap\USP17L2
1 of 14
No
No
Hsap\USP17L3
1 of 14
No
No
Hsap\USP17L4
1 of 14
No
No
Hsap\USP17L5
1 of 14
No
No
Hsap\USP17L6P
1 of 14
No
No
Hsap\USP17L7
1 of 14
No
No
Hsap\USP17L8
1 of 14
No
No
Hsap\USP17L10
1 of 14
No
No
Hsap\USP17L11
1 of 14
No
No
Hsap\USP17L12
1 of 14
No
No
Hsap\USP17L13
1 of 14
No
No
Hsap\USP17L15
1 of 14
No
No
Hsap\USP17L17
1 of 14
No
No
Hsap\USP17L18
1 of 14
No
No
Hsap\USP17L19
1 of 14
No
No
Hsap\USP17L20
1 of 14
No
No
Hsap\USP17L21
1 of 14
No
No
Hsap\USP17L22
1 of 14
No
No
Hsap\USP17L23
1 of 14
No
No
Hsap\USP17L24
1 of 14
No
No
Hsap\USP17L25
1 of 14
No
No
Hsap\USP17L26
1 of 14
No
No
Hsap\USP17L27
1 of 14
No
No
Hsap\USP17L28
1 of 14
No
No
Hsap\USP17L29
1 of 14
No
No
Hsap\USP17L30
1 of 14
No
No
Hsap\USP18
1 of 14
No
No
Hsap\USP19
1 of 14
No
No
Hsap\USP20
1 of 14
No
No
Hsap\USP21
1 of 14
No
No
Hsap\USP24
1 of 14
No
No
Hsap\USP26
1 of 14
No
No
Hsap\USP29
1 of 14
No
No
Hsap\USP32
1 of 14
No
No
2  
Hsap\USP33
1 of 14
No
No
Hsap\USP34
1 of 14
No
No
Hsap\USP35
1 of 14
No
No
Hsap\USP37
1 of 14
No
No
Hsap\USP38
1 of 14
No
No
Hsap\USP39
1 of 14
No
No
Hsap\USP41
1 of 14
No
No
Hsap\USP43
1 of 14
No
No
Hsap\USP45
1 of 14
No
No
Hsap\USP46
1 of 14
No
No
1  
Hsap\USP47
1 of 14
No
No
Hsap\USP48
1 of 14
No
No
Hsap\USP50
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (38)
Rnor\Usp22
14 of 14
Yes
Yes
Rnor\Usp51
12 of 14
No
Yes
Rnor\Usp27x
11 of 14
No
Yes
Rnor\Usp3
3 of 14
No
Yes
Rnor\Usp30
2 of 14
No
No
Rnor\Usp36
2 of 14
No
No
Rnor\Usp42
2 of 14
No
No
Rnor\Usp49
2 of 14
No
Yes
Rnor\LOC689730
1 of 14
No
No
Rnor\LOC100910107
1 of 14
No
No
Rnor\Usp2
1 of 14
No
No
Rnor\Usp4
1 of 14
No
No
Rnor\Usp5
1 of 14
No
No
Rnor\Usp8
1 of 14
No
No
Rnor\Usp9x
1 of 14
No
No
Rnor\Usp9y
1 of 14
No
No
Rnor\Usp11
1 of 14
No
No
Rnor\Usp12
1 of 14
No
No
Rnor\Usp13
1 of 14
No
No
Rnor\Usp14
1 of 14
No
No
Rnor\Usp15
1 of 14
No
No
Rnor\Usp16
1 of 14
No
No
Rnor\Usp18
1 of 14
No
No
Rnor\Usp19
1 of 14
No
No
Rnor\Usp20
1 of 14
No
No
Rnor\Usp21
1 of 14
No
No
Rnor\Usp24
1 of 14
No
No
Rnor\Usp32
1 of 14
No
No
Rnor\Usp33
1 of 14
No
No
Rnor\Usp34
1 of 14
No
No
Rnor\Usp38
1 of 14
No
No
Rnor\Usp39
1 of 14
No
No
Rnor\Usp44
1 of 14
No
Yes
Rnor\Usp45
1 of 14
No
No
Rnor\Usp46
1 of 14
No
No
Rnor\Usp47
1 of 14
No
No
Rnor\Usp48
1 of 14
No
No
Rnor\Usp50
1 of 14
No
No
Mus musculus (laboratory mouse) (41)
Mmus\Usp22
14 of 14
Yes
Yes
Mmus\Usp27x
12 of 14
No
Yes
Mmus\Usp51
12 of 14
No
Yes
Mmus\Usp3
3 of 14
No
Yes
Mmus\Usp30
2 of 14
No
No
Mmus\Usp36
2 of 14
No
No
Mmus\Usp49
2 of 14
No
Yes
Mmus\Usp2
1 of 14
No
No
Mmus\Usp4
1 of 14
No
No
Mmus\Usp5
1 of 14
No
No
Mmus\Usp8
1 of 14
No
No
Mmus\Usp9x
1 of 14
No
No
4  
Mmus\Usp9y
1 of 14
No
No
Mmus\Usp11
1 of 14
No
No
Mmus\Usp12
1 of 14
No
No
Mmus\Usp13
1 of 14
No
No
Mmus\Usp14
1 of 14
No
No
Mmus\Usp15
1 of 14
No
No
Mmus\Usp16
1 of 14
No
No
Mmus\Usp17la
1 of 14
No
No
Mmus\Usp17lb
1 of 14
No
No
Mmus\Usp17lc
1 of 14
No
No
Mmus\Usp17ld
1 of 14
No
No
Mmus\Usp17le
1 of 14
No
No
Mmus\Usp18
1 of 14
No
No
Mmus\Usp19
1 of 14
No
No
Mmus\Usp20
1 of 14
No
No
Mmus\Usp21
1 of 14
No
No
Mmus\Usp24
1 of 14
No
No
Mmus\Usp32
1 of 14
No
No
Mmus\Usp33
1 of 14
No
No
Mmus\Usp34
1 of 14
No
No
Mmus\Usp38
1 of 14
No
No
Mmus\Usp39
1 of 14
No
No
Mmus\Usp42
1 of 14
No
No
Mmus\Usp44
1 of 14
No
Yes
Mmus\Usp45
1 of 14
No
No
Mmus\Usp46
1 of 14
No
No
Mmus\Usp47
1 of 14
No
No
Mmus\Usp48
1 of 14
No
No
Mmus\Usp50
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (30)
Xtro\usp22
11 of 13
Yes
Yes
Xtro\btd
1 of 13
No
No
Xtro\itpkb
1 of 13
No
No
Xtro\LOC116410989
1 of 13
No
No
Xtro\tlk2
1 of 13
No
No
Xtro\usp3
1 of 13
No
Yes
Xtro\usp5
1 of 13
No
No
Xtro\usp10
1 of 13
No
No
Xtro\usp12b
1 of 13
No
No
Xtro\usp12
1 of 13
No
No
Xtro\usp13
1 of 13
No
No
Xtro\usp14
1 of 13
No
No
Xtro\usp15
1 of 13
No
No
Xtro\usp16
1 of 13
No
No
Xtro\usp19
1 of 13
No
No
Xtro\usp20
1 of 13
No
No
Xtro\usp21
1 of 13
No
No
Xtro\usp24
1 of 13
No
No
Xtro\usp30
1 of 13
No
No
Xtro\usp31
1 of 13
No
No
Xtro\usp33
1 of 13
No
No
Xtro\usp36
1 of 13
No
No
Xtro\usp38
1 of 13
No
No
Xtro\usp39
1 of 13
No
No
Xtro\usp42
1 of 13
No
No
Xtro\usp44
1 of 13
No
Yes
Xtro\usp45
1 of 13
No
No
Xtro\usp47
1 of 13
No
No
Xtro\usp48
1 of 13
No
No
Xtro\usp49
1 of 13
No
No
Danio rerio (Zebrafish) (33)
Drer\usp22
13 of 14
Yes
Yes
Drer\usp3
2 of 14
No
Yes
Drer\usp30
2 of 14
No
No
Drer\usp36
2 of 14
No
No
Drer\usp42
2 of 14
No
No
Drer\usp44
2 of 14
No
Yes
Drer\usp49
2 of 14
No
Yes
Drer\si:ch211-212k18.13
1 of 14
No
Yes
Drer\usp2a
1 of 14
No
No
Drer\usp2b
1 of 14
No
No
Drer\usp4
1 of 14
No
No
Drer\usp5
1 of 14
No
No
Drer\usp8
1 of 14
No
No
Drer\usp9
1 of 14
No
No
Drer\usp11
1 of 14
No
No
Drer\usp12a
1 of 14
No
No
Drer\usp12b
1 of 14
No
No
Drer\usp13
1 of 14
No
No
Drer\usp14
1 of 14
No
No
Drer\usp16
1 of 14
No
No
Drer\usp18
1 of 14
No
No
Drer\usp19
1 of 14
No
No
Drer\usp20
1 of 14
No
No
Drer\usp21
1 of 14
No
No
Drer\usp24
1 of 14
No
No
Drer\usp33
1 of 14
No
No
Drer\usp34
1 of 14
No
No
Drer\usp37
1 of 14
No
Yes
Drer\usp39
1 of 14
No
No
Drer\usp45
1 of 14
No
No
Drer\usp46
1 of 14
No
No
Drer\usp47
1 of 14
No
No
Drer\usp48
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (20)
Cele\usp-3
4 of 14
Yes
Yes
Cele\Y67D2.2
2 of 14
No
No
Cele\C04E6.5
1 of 14
No
No
Cele\C34F6.9
1 of 14
No
Yes
Cele\cyk-3
1 of 14
No
No
Cele\duo-1
1 of 14
No
Yes
Cele\duo-3
1 of 14
No
No
Cele\F07A11.4
1 of 14
No
No
Cele\H34C03.2
1 of 14
No
No
Cele\math-33
1 of 14
No
No
Cele\T05H10.1
1 of 14
No
No
Cele\T22F3.2
1 of 14
No
No
Cele\T24B8.7
1 of 14
No
No
Cele\usp-5
1 of 14
No
No
Cele\usp-14
1 of 14
No
No
Cele\usp-33
1 of 14
No
No
Cele\usp-39
1 of 14
No
No
Cele\usp-46
1 of 14
No
No
Cele\usp-48
1 of 14
No
No
Cele\usp-50
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (21)
Agam\AgaP_AGAP012119
11 of 12
Yes
Yes
Agam\AgaP_AGAP008805
3 of 12
No
Yes
Agam\AgaP_AGAP000269
2 of 12
No
No
Agam\AgaP_AGAP000666
1 of 12
No
No
Agam\AgaP_AGAP000884
1 of 12
No
No
Agam\AgaP_AGAP001052
1 of 12
No
No
Agam\AgaP_AGAP001209
1 of 12
No
No
Agam\AgaP_AGAP002900
1 of 12
No
No
Agam\AgaP_AGAP003643
1 of 12
No
No
Agam\AgaP_AGAP003821
1 of 12
No
No
Agam\AgaP_AGAP006652
1 of 12
No
No
Agam\AgaP_AGAP007106
1 of 12
No
No
Agam\AgaP_AGAP007521
1 of 12
No
No
Agam\AgaP_AGAP007600
1 of 12
No
No
Agam\AgaP_AGAP008381
1 of 12
No
No
Agam\AgaP_AGAP008530
1 of 12
No
No
Agam\AgaP_AGAP009348
1 of 12
No
No
Agam\AgaP_AGAP010080
1 of 12
No
No
Agam\AgaP_AGAP010088
1 of 12
No
No
Agam\AgaP_AGAP012139
1 of 12
No
No
Agam\AgaP_AGAP012248
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (30)
Atha\UBP22
12 of 13
Yes
Yes
Atha\AT4G22285
1 of 13
No
No
Atha\AT4G22350
1 of 13
No
No
Atha\AT4G22410
1 of 13
No
No
Atha\UBP1
1 of 13
No
No
Atha\UBP2
1 of 13
No
No
Atha\UBP3
1 of 13
No
No
Atha\UBP4
1 of 13
No
No
Atha\UBP5
1 of 13
No
No
Atha\UBP6
1 of 13
No
No
Atha\UBP7
1 of 13
No
No
Atha\UBP8
1 of 13
No
No
Atha\UBP9
1 of 13
No
No
Atha\UBP10
1 of 13
No
No
Atha\UBP11
1 of 13
No
No
Atha\UBP12
1 of 13
No
No
Atha\UBP13
1 of 13
No
No
Atha\UBP14
1 of 13
No
No
Atha\UBP15
1 of 13
No
No
Atha\UBP16
1 of 13
No
No
Atha\UBP17
1 of 13
No
No
Atha\UBP18
1 of 13
No
No
Atha\UBP19
1 of 13
No
No
Atha\UBP20
1 of 13
No
No
Atha\UBP21
1 of 13
No
No
Atha\UBP23
1 of 13
No
No
Atha\UBP24
1 of 13
No
No
Atha\UBP25
1 of 13
No
No
Atha\UBP26
1 of 13
No
No
Atha\UBP27
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (15)
Scer\UBP8
12 of 13
Yes
Yes
Scer\UBP1
2 of 13
No
No
Scer\DOA4
1 of 13
No
No
1  
Scer\SAD1
1 of 13
No
No
Scer\UBP3
1 of 13
No
No
Scer\UBP5
1 of 13
No
No
Scer\UBP6
1 of 13
No
No
Scer\UBP7
1 of 13
No
No
Scer\UBP9
1 of 13
No
No
Scer\UBP10
1 of 13
No
No
Scer\UBP11
1 of 13
No
No
Scer\UBP12
1 of 13
No
No
Scer\UBP13
1 of 13
No
No
Scer\UBP14
1 of 13
No
No
Scer\UBP16
1 of 13
No
Yes
Schizosaccharomyces pombe (Fission yeast) (12)
Spom\ubp8
11 of 12
Yes
Yes
Spom\ubp11
3 of 12
No
Yes
Spom\ubp16
2 of 12
No
No
Spom\ubp4
1 of 12
No
No
Spom\ubp5
1 of 12
No
No
Spom\ubp6
1 of 12
No
No
Spom\ubp7
1 of 12
No
No
Spom\ubp9
1 of 12
No
No
Spom\ubp10
1 of 12
No
No
Spom\ubp12
1 of 12
No
No
Spom\ubp14
1 of 12
No
No
Spom\ubp15
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:not. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (20)
Usp2
3 of 13
Usp8
3 of 13
Usp15-31
3 of 13
Usp30
3 of 13
DUBAI
2 of 13
faf
2 of 13
scny
2 of 13
Usp5
2 of 13
Usp7
2 of 13
Usp10
2 of 13
Usp14
2 of 13
Usp16-45
2 of 13
Usp20-33
2 of 13
Usp32
2 of 13
Usp39
2 of 13
Usp47
2 of 13
HDAC6
1 of 13
puf
1 of 13
Usp1
1 of 13
Usp12-46
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    USP22; ubiquitin specific peptidase 22
    14 of 14
    612116
        USP27X; ubiquitin specific peptidase 27 X-linked
        12 of 14
        300975
        USP51; ubiquitin specific peptidase 51
        12 of 14
            USP3; ubiquitin specific peptidase 3
            3 of 14
            604728
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Interactions
                Summary of Physical Interactions
                Interaction Browsers
                View in FlyBase Interactions Browser   View in MIST tool (external link)
                Please see the Physical Interaction reports below for full details
                protein-protein
                Physical Interaction
                Assay
                References
                not - Ada1-2
                anti tag coimmunoprecipitation, peptide massfingerprinting
                (Weake et al., 2009)
                not - Ada2b
                anti tag coimmunoprecipitation, peptide massfingerprinting, western blot
                (Cloud et al., 2019, Weake et al., 2011, Weake et al., 2009)
                not - Atxn7
                anti tag coimmunoprecipitation, peptide massfingerprinting, western blot
                (Cloud et al., 2019, Mohan et al., 2014)
                not - Brf
                anti bait coimmunoprecipitation, western blot
                (Popova et al., 2018)
                not - Cp190
                anti bait coimmunoprecipitation, western blot, pull down, two hybrid, anti tag coimmunoprecipitation, molecular sieving, Identification by mass spectrometry
                (Erez et al., 2021)
                not - Gcn5
                anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot
                (Mohan et al., 2014, Gurskiy et al., 2012, Weake et al., 2008)
                not - Pbp45
                anti bait coimmunoprecipitation, western blot
                (Popova et al., 2018)
                not - SCAR
                anti tag coimmunoprecipitation, western blot, tandem affinity purification, molecular sieving, multidimensional protein identification technology, anti bait coimmunoprecipitation
                (Cloud et al., 2019)
                not - Saf6
                anti tag coimmunoprecipitation, peptide massfingerprinting
                (Weake et al., 2009)
                not - Sf3b5
                anti tag coimmunoprecipitation, peptide massfingerprinting
                (Stegeman et al., 2016)
                not - Sgf11
                anti tag coimmunoprecipitation, molecular sieving, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot, anti tag western blot, three hybrid
                (Erez et al., 2021, Popova et al., 2018, Mohan et al., 2014, Gurskiy et al., 2012, Weake et al., 2008)
                not - Sgf29
                anti tag coimmunoprecipitation, peptide massfingerprinting
                (Weake et al., 2009)
                not - Spt20
                anti tag coimmunoprecipitation, peptide massfingerprinting
                (Stegeman et al., 2016)
                not - Spt3
                anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot
                (Stegeman et al., 2016, Weake et al., 2008)
                not - e(y)2
                anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation, molecular sieving, Identification by mass spectrometry, three hybrid
                (Erez et al., 2021, Popova et al., 2018, Gurskiy et al., 2012)
                not - mod(mdg4)
                transcriptional complementation assay, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, molecular sieving, Identification by mass spectrometry
                (Erez et al., 2021)
                not - stumps
                two hybrid
                (Battersby et al., 2003)
                not - wda
                anti tag coimmunoprecipitation, peptide massfingerprinting
                (Weake et al., 2009)
                Summary of Genetic Interactions
                Interaction Browsers
                View in FlyBase Interactions Browser   View in MIST tool (external link)
                Please look at the allele data for full details of the genetic interactions
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                not
                suppressible
                cpb
                (Badmos et al., 2021)
                not
                enhanceable
                htt
                (Dietz et al., 2015)
                not
                enhanceable
                Prosβ6
                (Poeck et al., 2001)
                not
                suppressible
                SCAR
                (Badmos et al., 2021)
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                peb
                suppressible
                not
                (Wilk et al., 2004)
                External Data
                Subunit Structure (UniProtKB)
                Component of the SAGA transcription coactivator-HAT complex, at least composed of Ada2b, not/nonstop, Pcaf/Gcn5, Sgf11 and Spt3.
                (UniProt, Q9VVR1 )
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                DroID - A comprehensive database of gene and protein interactions.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                FlyBase
                External Links
                External Data
                Linkouts
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Class of Gene
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                3L
                Recombination map
                3-45
                Cytogenetic map
                75D4-75D4
                Sequence location
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                75D4-75D4
                Limits computationally determined from genome sequence between P{PZ}W05014 and P{lacW}l(3)j14E7j14E7
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                75C1-75C2
                (Poeck et al., 2001)
                75C1-75C4
                (Poeck, 1999.8.23)
                75C3-75C4
                (determined by in situ hybridisation)
                (Spradling et al., 1999)
                75C1-75C4
                (determined by in situ hybridisation)
                (Martin et al., 1995)
                63A3-63A4
                75C1--2
                (BDGP Project Members, 1994-1999)
                Mapped by recombination analysis and deficiency complementation tests (details unspecified).
                (Poeck et al., 2001)
                Experimentally Determined Recombination Data
                Location

                3-45

                (FlyBase, 2016)

                3-41.8

                (Comeron et al., 2012)

                3-

                (Martin et al., 1995)
                Left of (cM)
                Right of (cM)
                (Martin et al., 1995)
                Notes
                Stocks and Reagents
                Stocks (14)
                Genomic Clones (13)
                 

                Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                cDNA Clones (27)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                  Drosophila Genomics Resource Center cDNA clones

                  For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                  cDNA Clones, End Sequenced (ESTs)
                  BDGP DGC clones
                  Other clones
                  RNAi and Array Information
                  Linkouts
                  DRSC - Results frm RNAi screens
                  Antibody Information
                  Laboratory Generated Antibodies
                   

                  polyclonal

                  (Li et al., 2017, Gurskiy et al., 2012)
                  Commercially Available Antibodies
                   
                  Cell Line Information
                  Publicly Available Cell Lines
                   
                    Other Stable Cell Lines
                     
                    Other Comments

                    Area matching Drosophila EST AA264796 (inverted).

                    (Davies, 2001.3.15)

                    The phenotype of not mutants suggests that not is required for the migration of the epithelial, marginal and medulla glial cells in the developing optic lobe.

                    (Poeck et al., 2001)

                    Genetic mosaic analysis fails to reveal a retinal requirement for not. Phenotypic analysis suggests not is not required in the optic ganglia to specify the correct termination site of R1-R6 neurons.

                    (Martin et al., 1995)
                    Relationship to Other Genes
                    Source for database merge of

                    Source for merge of: not l(3)02069

                    (Salecker, 2001.3.5)

                    Source for merge of: not anon-WO0257455.9

                    (FlyBase, 1996-)
                    Additional comments

                    Source for merge of not anon-WO0257455.9 was sequence comparison ( date:051113 ).

                    (FlyBase, 1996-)
                    Nomenclature History
                    Source for database identify of
                    Nomenclature comments
                    Etymology
                    Synonyms and Secondary IDs (18)
                    Reported As
                    Symbol Synonym
                    CG4166
                    (Pai et al., 2023, Páhi et al., 2022, Brewer-Jensen et al., 2016, Stegeman et al., 2016, Zhou et al., 2015, Engel et al., 2014, Mohan et al., 2014, Tsou et al., 2012, Zhang et al., 2012, Ardehali et al., 2009, Perkins et al., 2009, Berger et al., 2008, Battersby et al., 2003)
                    NOT
                    (Barckmann and Simonelig, 2013, Xia et al., 2012)
                    Non-stop
                    (Cornelio-Parra et al., 2021)
                    Nonstop
                    (Popova et al., 2018, Spedale et al., 2012, Rodriguez-Navarro, 2009)
                    Not
                    (Erez et al., 2021, Torres-Zelada and Weake, 2021, Cacchione et al., 2020, Zhou et al., 2015, Engel et al., 2014, Zhang et al., 2012)
                    Q9VVR1
                    (Clague et al., 2013)
                    UBP8
                    (Weake et al., 2008)
                    USP22
                    (Weake et al., 2008)
                    Upb8
                    (Ardehali et al., 2009)
                    anon-WO0257455.9
                    dNonStop
                    (Spedale et al., 2012)
                    dNonstop
                    (Helmlinger et al., 2021)
                    i155
                    (Battersby et al., 2003)
                    l(3)02069
                    (Beaton, 1999.12.12, Spradling et al., 1999, Addison et al., 1995, BDGP Project Members, 1994-1999)
                    l(3)j9A6
                    non-stop
                    (Kim et al., 2020, Cloud et al., 2019)
                    nonstop
                    (Martin et al., 1995)
                    not
                    (Zhao et al., 2024, Liu et al., 2023, Harris-Gauthier et al., 2022, Páhi et al., 2022, Badmos et al., 2021, Mahesh et al., 2020, Wei et al., 2020, Cloud et al., 2019, Hall et al., 2019, Meltzer, 2019-, Meltzer et al., 2019, Gene Disruption Project members, 2018-, Li et al., 2017, Ma et al., 2016, Stegeman et al., 2016, Dietz et al., 2015, Rao et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Tsou et al., 2012, Gan et al., 2010, Ardehali et al., 2009, Christensen et al., 2009.5.6, Berger et al., 2008, Christensen et al., 2008.4.15, Weake et al., 2008, Zhao et al., 2008, Garcia et al., 2007, Kaplow et al., 2007)
                    Name Synonyms
                    Non-stop
                    (Boumard and Bardin, 2021, Erez et al., 2021, Mohan et al., 2014)
                    Nonstop
                    (Gurskiy et al., 2012, Weake et al., 2011, Weake et al., 2009)
                    non-stop
                    (Li et al., 2017, Berger et al., 2008, Garcia et al., 2007)
                    nonstop
                    (Stegeman et al., 2016, Hadjieconomou et al., 2011, Hoopfer et al., 2008, Weake et al., 2008, Zhao et al., 2008, Kaplow et al., 2007, Chotard and Salecker, 2004, Pielage and Klambt, 2001)
                    Secondary FlyBase IDs
                    • FBgn0010766
                    • FBgn0011415
                    • FBgn0063787
                    Datasets (0)
                    Study focus (0)
                    Experimental Role
                    Project
                    Project Type
                    Title
                    Study result (0)
                    Result
                    Result Type
                    Title
                    External Crossreferences and Linkouts ( 55 )
                    Sequence Crossreferences
                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                    Other crossreferences
                    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                    FlyMine - An integrated database for Drosophila genomics
                    InterPro - A database of protein families, domains and functional sites
                    KEGG Genes - Molecular building blocks of life in the genomic space.
                    MARRVEL_MODEL - MARRVEL (model organism gene)
                    Linkouts
                    BioGRID - A database of protein and genetic interactions.
                    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                    DroID - A comprehensive database of gene and protein interactions.
                    DRSC - Results frm RNAi screens
                    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                    Flygut - An atlas of the Drosophila adult midgut
                    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                    References (123)