FB2022_02, released March 29, 2022

Gene: Dmel\nuf

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General Information
Symbol
Dmel\nuf
Species
D. melanogaster
Name
nuclear fallout
Annotation Symbol
CG33991
Feature Type
protein_coding_gene
FlyBase ID
FBgn0013718
Gene Model Status
Current
Stock Availability
25 publicly available
Gene Summary
nuclear fallout (nuf) encodes a cytoplasmic, coiled-coil protein that acts as an adaptor between the product of Rab11 and motor proteins in the recycling endosome pathway. It is required for early embryo cellularization, and for subsequent Rab11-dependent processes. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

D2, Corkscrew Interacting Protein-D2, Cy3-23, CIP-D2, anon-D2

Key Links
Genomic Location
Cytogenetic map
70D3-70D4
Sequence location
Recombination map
3-42
RefSeq locus
NT_037436 REGION:14190870..14233250
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in actin cytoskeleton reorganization
inferred from mutant phenotype
(Riggs et al., 2003)
involved_in sensory organ precursor cell fate determination
inferred from mutant phenotype
(Emery et al., 2005)
involved_in wing disc dorsal/ventral pattern formation
inferred from genetic interaction with FLYBASE:Bx; FB:FBgn0265598, FLYBASE:ap; FB:FBgn0267978
(Bejarano et al., 2008)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in endocytic recycling
inferred from biological aspect of ancestor with PANTHER:PTN001039242
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in regulation of cytokinesis
inferred from biological aspect of ancestor with PANTHER:PTN001039242
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (8 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in apical cortex
inferred from direct assay
(Singari et al., 2014)
colocalizes_with centrosome
inferred from direct assay
(Emery et al., 2005)
colocalizes_with microtubule organizing center
inferred from direct assay
(Riggs et al., 2007)
located_in perinuclear region of cytoplasm
inferred from direct assay
(Giansanti et al., 2007)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
located_in centrosome
traceable author statement
(Tepass et al., 2001)
is_active_in cleavage furrow
inferred from biological aspect of ancestor with PANTHER:PTN001039242
(assigned by GO_Central )
(Gaudet et al., 2011)
is_active_in endocytic vesicle
inferred from biological aspect of ancestor with PANTHER:PTN001039242
(assigned by GO_Central )
(Gaudet et al., 2011)
is_active_in midbody
inferred from biological aspect of ancestor with PANTHER:PTN001039242
(assigned by GO_Central )
(Gaudet et al., 2011)
is_active_in recycling endosome membrane
inferred from biological aspect of ancestor with PANTHER:PTN001039242
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
nuclear fallout (nuf) encodes a cytoplasmic, coiled-coil protein that acts as an adaptor between the product of Rab11 and motor proteins in the recycling endosome pathway. It is required for early embryo cellularization, and for subsequent Rab11-dependent processes. [Date last reviewed: 2019-03-14]
Summary (Interactive Fly)

pericentrosomal protein which, along with Rab11, is involved in membrane trafficking and actin remodeling during membrane furrow formation during cellularization - involved in endosomal-mediated membrane delivery to the site of furrow formation

Gene Model and Products
Number of Transcripts
12
Number of Unique Polypeptides
9

Please see the JBrowse view of Dmel\nuf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry M9ND89)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
nuf-RA
FBtr0100040
NM_001038922
2696
502
nuf-RB
FBtr0100042
NM_001038918
2373
305
nuf-RC
FBtr0100039
NM_001038920
3446
502
nuf-RD
FBtr0100041
NM_001038921
2448
316
nuf-RE
FBtr0100043
NM_001038919
3233
528
nuf-RF
FBtr0100044
NM_001038917
3099
528
nuf-RG
FBtr0306619
NM_001259820
3533
530
nuf-RH
FBtr0306620
NM_001259821
3530
529
nuf-RJ
FBtr0306622
NM_001259822
3272
541
nuf-RK
FBtr0306623
NM_001259823
3138
541
nuf-RL
FBtr0306624
NM_001259824
2735
515
nuf-RM
FBtr0307191
NM_001259819
2707
318
Additional Transcript Data and Comments
Reported size (kB)

3.5, 2.4 (northern blot)

(Rothwell et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
nuf-PA
FBpp0075474
57.1
502
4.76
NP_001034011
AAF49749
nuf-PB
FBpp0075476
36.1
305
4.64
NP_001034007
AAN11807
nuf-PC
FBpp0075475
57.1
502
4.76
NP_001034009
ABC66147
nuf-PD
FBpp0089174
37.3
316
4.72
NP_001034010
AAS65011
nuf-PE
FBpp0100130
59.9
528
4.53
NP_001034008
ABC66148
nuf-PF
FBpp0100129
59.9
528
4.53
NP_001034006
ABC66149
nuf-PG
FBpp0297574
60.2
530
4.80
NP_001246749
AFH04420
nuf-PH
FBpp0297575
60.1
529
4.80
NP_001246750
AFH04421
nuf-PJ
FBpp0297577
61.1
541
4.49
NP_001246751
AFH04422
nuf-PK
FBpp0297578
61.1
541
4.49
NP_001246752
AFH04423
nuf-PL
FBpp0297579
58.3
515
4.70
NP_001246753
AFH04424
nuf-PM
FBpp0298020
37.4
318
4.57
NP_001246748
AFH04419
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

502 aa isoforms: nuf-PA, nuf-PC
528 aa isoforms: nuf-PE, nuf-PF
541 aa isoforms: nuf-PJ, nuf-PK
Additional Polypeptide Data and Comments
Reported size (kDa)

502 (aa); 57 (kD predicted)

(Rothwell et al., 1998)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nuf using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
ventral midline of embryo
(Wheeler et al., 2009)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
anlage in statu nascendi
(Fisher et al., 2012)
central brain anlage in statu nascendi
(Fisher et al., 2012)
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
cardiogenic mesoderm
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
dorsal vessel primordium

Comment: reported as dorsal vessel specific anlage

(Fisher et al., 2012)
salivary gland body primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
stomatogastric nervous system primordium
(Fisher et al., 2012)
embryonic stage 11 -- 13
cardiogenic mesoderm
(Ahmad et al., 2012)
embryonic stage 13 -- 16
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic/larval dorsal vessel
(Fisher et al., 2012)
embryonic/larval salivary gland body
(Fisher et al., 2012)
embryonic stage 16 -- 17
embryonic/larval heart
(Ahmad et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 6

Comment: reference states 0-3 hr AEL

(Rothwell et al., 1998)
Additional Descriptive Data

nuf transcripts are detected in 0-3 hr embryos.

(Rothwell et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis & adult stage | female
main body follicle cell | apical
(Khanal et al., 2016)
spermatogenesis & adult stage | male
spermatocyte

Comment: 0-2 days post eclosion

(Polevoy et al., 2009)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 6

Comment: reference states 0-3 hr AEL

(Rothwell et al., 1998)
embryonic stage 4
(Rothwell et al., 1998)
embryonic stage 5
(Rothwell et al., 1998)
Additional Descriptive Data

A major band of 95 kD and minor bands of 82 and 67 kD are detected in Western blots of 0-3 hr embryos. Following phosphatase treatment, only a single 67 kD band is observed. During late syncytial divisions, nuf protein is highly concentrated at the centrosomes during prophase, and is cytoplasmic during other stages of the nuclear cycle. nuf protein is also concentrated at the centrosomes during interphase of nuclear cycle 14, and during cellularization.

(Rothwell et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in apical cortex
inferred from direct assay
(Singari et al., 2014)
colocalizes_with centrosome
inferred from direct assay
(Emery et al., 2005)
colocalizes_with microtubule organizing center
inferred from direct assay
(Riggs et al., 2007)
located_in perinuclear region of cytoplasm
inferred from direct assay
(Giansanti et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\nuf in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 35 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of nuf
Transgenic constructs containing regulatory region of nuf
Aberrations (Deficiencies and Duplications) ( 4 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | maternal effect | recessive
lethal - all die during embryonic stage | maternal effect
lethal - all die during embryonic stage | maternal effect (with nuf1)
lethal - all die during embryonic stage | maternal effect (with nufCy3-23)
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
Other Phenotypes
Allele
abnormal neuroanatomy | larval stage, with Scer\GAL4elav-C155
some die during embryonic stage | maternal effect
some die during embryonic stage | maternal effect (with nuf1)
some die during embryonic stage | maternal effect (with nufCy3-23)
some die during pupal stage, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4Bx-MS1096-KE
visible, with Scer\GAL4ey.PH
visible, with Scer\GAL4GMR.PF
visible, with Scer\GAL4sca-537.4
wild-type
Phenotype manifest in
Allele
actin cytoskeleton | embryonic stage
anterior fascicle & synapse, with Scer\GAL4elav-C155
anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155
cleavage furrow
embryo | embryonic cycle 11 | maternal effect
embryo | embryonic cycle 12 | maternal effect
embryo | embryonic cycle 13 | maternal effect
embryo | embryonic cycle 14 | maternal effect
eye, with Scer\GAL4ey.PH
eye, with Scer\GAL4GMR.PF
larval intersegmental nerve, with Scer\GAL4elav-C155
macrochaeta
mesothoracic bristle | adult stage | increased number, with Scer\GAL4sca.PU
mitotic telophase | maternal effect
nucleus | embryonic stage
scutellar bristle, with Scer\GAL4sca-537.4
scutellar bristle | ectopic, with Scer\GAL4sca-537.4
tormogen cell | increased number, with Scer\GAL4sca-109-68
trichogen cell, with Scer\GAL4sca-109-68
trichogen cell | ectopic, with Scer\GAL4sca-537.4
wing, with Scer\GAL4Bx-MS1096-KE
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\RAB11FIP4
11 of 15
Yes
Yes
1  
Hsap\RAB11FIP3
10 of 15
No
Yes
Hsap\MIR4724
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Rab11fip4
12 of 15
Yes
Yes
Mmus\Rab11fip3
10 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Rab11fip4
10 of 13
Yes
Yes
Rnor\Rab11fip3
3 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\rab11fip4
7 of 12
Yes
Yes
Xtro\rab11fip3
5 of 12
No
Yes
Xtro\rab11fip4l
2 of 12
No
Yes
Xtro\ppfia1
1 of 12
No
No
Danio rerio (Zebrafish) (3)
Drer\rab11fip4a
11 of 15
Yes
Yes
Drer\rab11fip4b
9 of 15
No
Yes
Drer\rab11fip3
8 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\rfip-1
12 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    RAB11FIP4; RAB11 family interacting protein 4
    11 of 15
    611999
         
        RAB11FIP3; RAB11 family interacting protein 3
        10 of 15
        608738
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            nuf
            two hybrid
            (Hu et al., 2017.6.13)
            nuf - 14-3-3ε
            pull down, western blot
            (Calero-Cuenca et al., 2016)
            nuf - CG15728
            two hybrid
            (Hu et al., 2017.6.13)
            nuf - DCTN1-p150
            pull down, western blot
            (Calero-Cuenca et al., 2016)
            nuf - Dhc64C
            anti tag coimmunoprecipitation, western blot
            (Riggs et al., 2007)
            nuf - Dlic
            pull down, western blot, molecular weight estimation by staining
            (Calero-Cuenca et al., 2016, Otani et al., 2011)
            nuf - Gsc
            two hybrid
            (Hu et al., 2017.6.13)
            nuf - IKKε
            enzymatic study, molecular weight estimation by staining, pull down, western blot
            (Otani et al., 2011)
            nuf - Khc
            pull down, western blot
            (Calero-Cuenca et al., 2016)
            nuf - Rab11
            pull down, peptide massfingerprinting, molecular weight estimation by staining, anti tag western blot, western blot, colocalization, fluorescence microscopy, inferred by author
            (Calero-Cuenca et al., 2016, Gillingham et al., 2014, Singari et al., 2014, Ramel et al., 2013, Otani et al., 2011, Riggs et al., 2003)
            nuf - aPKC
            pull down, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, protein kinase assay, autoradiography
            (Calero-Cuenca et al., 2016)
            nuf - dx
            two hybrid
            (Hu et al., 2017.6.13)
            nuf - polo
            pull down, western blot, enzymatic study, autoradiography
            (Brose et al., 2017)
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            nuf
            enhanceable
            l(2)gl
            (Emery et al., 2005)
            nuf
            enhanceable
            Rab11
            (Emery et al., 2005)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Bx
            enhanceable
            nuf
            (Bejarano et al., 2008)
            Bx|ap
            suppressible
            nuf
            (Bejarano et al., 2008)
            IKKε
            suppressible
            nuf
            (Otani et al., 2011)
            l(2)gl
            enhanceable
            nuf
            (Emery et al., 2005)
            lap
            enhanceable
            nuf
            (Sun et al., 2006)
            pbl
            suppressible
            nuf
            (Gregory et al., 2007)
            Rab11
            enhanceable
            nuf
            (Emery et al., 2005)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            External Data
            Linkouts
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3L
            Recombination map
            3-42
            Cytogenetic map
            70D3-70D4
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            70D3-70D4
            Limits computationally determined from genome sequence between P{PZ}l(3)70Da02402&P{PZ}btl00208 and P{PZ}Mpcp00564
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            70D3-70D3
            (determined by in situ hybridisation)
            (Abdelilah-Seyfried et al., 2000)
            70D3-70D3
            (Rothwell, 1998.1.27, Rothwell et al., 1996)
            70-70
            (determined by in situ hybridisation)
            (Sullivan et al., 1993)
            Experimentally Determined Recombination Data
            Location

            3-42

            (FlyBase, 2016)

            3-35.8

            (Comeron et al., 2012)

            3-

            (Rothwell et al., 1996)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (25)
            Genomic Clones (34)
            cDNA Clones (146)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Calero-Cuenca et al., 2016, Otani et al., 2011, Rothwell et al., 1998)
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: nuf CG7867

            (Bayraktaroglu, 1999.11)
            Source for database merge of

            Source for merge of: nuf CIP-D2 anon-D2

            (Perkins, 2001.9.13)

            Source for merge of: nuf EP3077

            (FlyBase Genome Annotators, 2005, FlyBase, 1996-)
            Additional comments

            Source for merge of nuf EP3077 was location of EP3077 insertion ( date:020218 ).

            (FlyBase Genome Annotators, 2005, FlyBase, 1996-)
            Other Comments

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)

            New annotation (CG32140) in release 3 of the genome annotation.

            (FlyBase Genome Annotators, 2002-2003)

            The nuf-mutant phenotype is used to investigate the process by which a furrow is constructed.

            (Rothwell et al., 1998)

            Protein interacts specifically with csw unique catalytic insert domain.

            (Johnson et al., 1997)

            Identification: Yeast two hybrid screen for proteins that interact with the csw protein's protein phosphatase domain.

            (Denhez et al., 1996)

            nuf gene product has a zinc finger motif.

            (Peterson and Petri, 1995)

            Deficiency analysis localized nuf to 70D2-70D6.

            (Sullivan et al., 1993)

            Defined in a screen of maternal-effect lethal mutations that cause defects in nuclear movement, organization or morphology during the syncytial embryonic divisions.

            (Sullivan et al., 1993)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 117 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            modMine - A data warehouse for the modENCODE project
            Linkouts
            ApoDroso - Functional genomic database for photoreceptor development, survival and function
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Synonyms and Secondary IDs (21)
            Reported As
            Symbol Synonym
            CG32140
            (Giot et al., 2003)
            CG33991
            (Ramel et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, FlyBase Genome Annotators, 2005)
            CG3779
            (Ahmad et al., 2012)
            CG7867
            (Ahmad et al., 2012, Norga et al., 2003, Goldstein and Gunawardena, 2000)
            CIP-D2
            (Perkins, 2001.9.13)
            Cy3-23
            (Peterson and Petri, 1995)
            D2
            (Lorenzen et al., 1998, Johnson et al., 1997, Denhez et al., 1996)
            EP3077
            FIP3
            (van IJzendoorn, 2006)
            NUF
            (Lecuit, 2004)
            Nuf
            (Brose et al., 2017, Le Droguen et al., 2015, Singari et al., 2014, Yashiro et al., 2014, Fabian and Brill, 2012, Kitazawa et al., 2012, Otani et al., 2011, Sommi et al., 2010, Kao and Megraw, 2009, Polevoy et al., 2009, Cao et al., 2008, Crest and Sullivan, 2008, McCleland and O'Farrell, 2008, Shaye et al., 2008, Giansanti et al., 2007, Riggs et al., 2007, Slack et al., 2007, van IJzendoorn, 2006, Kim et al., 2005, Langevin et al., 2005, Sevrioukov et al., 2005, Somers and Chia, 2005, Perkins, 2001.9.13)
            Nuf1
            (Xu et al., 2011)
            Nuf2p
            (White and Erickson, 2009)
            anon-D2
            (Perkins, 2001.9.13)
            nuf
            (White et al., 2020, Blake-Hedges and Megraw, 2019, Story et al., 2019, Hu et al., 2017.6.13, Yan et al., 2017, Clandinin and Owens, 2016-, Duff et al., 2015, Gene Disruption Project members, 2015-, Acharya et al., 2014, Ashwal-Fluss et al., 2014, DeSalvo et al., 2014, Singari et al., 2014, Esslinger et al., 2013, Gault et al., 2012, Izutsu et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Abruzzi et al., 2011, Carreira et al., 2011, Friedman et al., 2011, Hoskins et al., 2011, Karbowniczek et al., 2010, Reis et al., 2010, Christensen et al., 2009.5.6, Fang et al., 2009, Hazelett et al., 2009, Wheeler et al., 2009, Bejarano et al., 2008, Morozova et al., 2007, Sandmann et al., 2007, Liebl et al., 2006, Emery et al., 2005)
            Name Synonyms
            Corkscrew Interacting Protein-D2
            (Perkins, 2001.9.13)
            Nuclear Fallout
            (Thomas and Noselli, 2007)
            Nuclear fallout
            (Ramel et al., 2013, Fabian and Brill, 2012, Cao et al., 2008, Slack et al., 2007, Polo and Di, 2006, van IJzendoorn, 2006)
            Nuclear-fallout
            (McCleland and O'Farrell, 2008, Riggs et al., 2007)
            nuclear fallout
            (Pogson et al., 2014, Singari et al., 2014, Gault et al., 2012, Gould, 2011, Karbowniczek et al., 2010, Gregory et al., 2007, Morozova et al., 2007, Morozova et al., 2006, Burnette et al., 2005, Perkins, 2001.9.13)
            nuclear-fallout
            (Lecuit, 2004, Scaria et al., 2004, Sullivan et al., 1993)
            Secondary FlyBase IDs
            • FBgn0013768
            • FBgn0015626
            • FBgn0024703
            • FBgn0044464
            • FBgn0052140
            • FBgn0053991
            • FBtr0075731
            • FBtr0075732
            • FBtr0075733
            • FBtr0075734
            • FBtr0075738
            • FBpp0075480
            • FBtr0075739
            • FBpp0075481
            Datasets (8)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (8)
            Result
            Result Type
            Title
            scRNAseq_2020_Cattenoz_NI_seq_clustering
            Clustering analysis of hemocytes from non-infested third instar larvae
            scRNAseq_2020_Cattenoz_WI_seq_clustering
            Clustering analysis of hemocytes from wasp-infested third instar larvae
            scRNAseq_2020_Cho_NI72LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
            scRNAseq_2020_Cho_NI96LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI120LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
            scRNAseq_2020_Cho_WI96LG_seq_clustering
            Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI96HL_seq_clustering
            Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI120HL_seq_clustering
            Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
            References (145)