FlyBase Gene Report: Dmel\nuf

General Information

Symbol

Dmel\nuf

Species

D. melanogaster

Name

nuclear fallout

Annotation Symbol

CG33991

Feature Type

protein_coding_gene

FlyBase ID

FBgn0013718

Gene Model Status

Current

Stock Availability

27 publicly available

Gene Snapshot

nuclear fallout (nuf) encodes a cytoplasmic, coiled-coil protein that acts as an adaptor between the product of Rab11 and motor proteins in the recycling endosome pathway. It is required for early embryo cellularization, and for subsequent Rab11-dependent processes. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

D2, Corkscrew Interacting Protein-D2, CIP-D2, Cy3-23, anon-D2

Key Links

Genomic Location

Cytogenetic map

70D3-70D4

Sequence location

3L:14,190,870..14,233,250 [+]

Recombination map

3-42

RefSeq locus

NT_037436 REGION:14190870..14233250

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Protein Family (UniProt)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

Predictions / Assertions

ADP-ribosylation factor binding; Rab GTPase binding

Summaries

Summary (Interactive Fly)

pericentrosomal protein which, along with Rab11, is involved in membrane trafficking and actin remodeling during membrane furrow formation during cellularization - involved in endosomal-mediated membrane delivery to the site of furrow formation

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\nuf or the JBrowse view of Dmel\nuf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

nuf-RA

FBtr0100040

NM_001038922

2696

502

nuf-RB

FBtr0100042

NM_001038918

2373

305

nuf-RC

FBtr0100039

NM_001038920

3446

502

nuf-RD

FBtr0100041

NM_001038921

2448

316

nuf-RE

FBtr0100043

NM_001038919

3233

528

nuf-RF

FBtr0100044

NM_001038917

3099

528

nuf-RG

FBtr0306619

NM_001259820

3533

530

nuf-RH

FBtr0306620

NM_001259821

3530

529

nuf-RJ

FBtr0306622

NM_001259822

3272

541

nuf-RK

FBtr0306623

NM_001259823

3138

541

nuf-RL

FBtr0306624

NM_001259824

2735

515

nuf-RM

FBtr0307191

NM_001259819

2707

318

Additional Transcript Data and Comments

Reported size (kB)

3.5, 2.4 (northern blot)

(Rothwell et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

nuf-PA

FBpp0075474

57.1

502

4.76

NP_001034011

AAF49749

nuf-PB

FBpp0075476

36.1

305

4.64

NP_001034007

AAN11807

nuf-PC

FBpp0075475

57.1

502

4.76

NP_001034009

ABC66147

nuf-PD

FBpp0089174

37.3

316

4.72

NP_001034010

AAS65011

nuf-PE

FBpp0100130

59.9

528

4.53

NP_001034008

ABC66148

nuf-PF

FBpp0100129

59.9

528

4.53

NP_001034006

ABC66149

nuf-PG

FBpp0297574

60.2

530

4.80

NP_001246749

AFH04420

nuf-PH

FBpp0297575

60.1

529

4.80

NP_001246750

AFH04421

nuf-PJ

FBpp0297577

61.1

541

4.49

NP_001246751

AFH04422

nuf-PK

FBpp0297578

61.1

541

4.49

NP_001246752

AFH04423

nuf-PL

FBpp0297579

58.3

515

4.70

NP_001246753

AFH04424

nuf-PM

FBpp0298020

37.4

318

4.57

NP_001246748

AFH04419

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

502 aa isoforms: nuf-PA, nuf-PC

528 aa isoforms: nuf-PE, nuf-PF

541 aa isoforms: nuf-PJ, nuf-PK

Additional Polypeptide Data and Comments

Reported size (kDa)

502 (aa); 57 (kD predicted)

(Rothwell et al., 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nuf using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (15 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

ADP-ribosylation factor binding

inferred from biological aspect of ancestor with PANTHER:PTN001039242

(assigned by GO_Central )

(Gaudet et al., 2011)

Rab GTPase binding

inferred from biological aspect of ancestor with PANTHER:PTN001039242

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

actin cytoskeleton reorganization

inferred from mutant phenotype

(Riggs et al., 2003)

sensory organ precursor cell fate determination

inferred from mutant phenotype

(Emery et al., 2005)

wing disc dorsal/ventral pattern formation

inferred from genetic interaction with FLYBASE:Bx; FB:FBgn0265598, FLYBASE:ap; FB:FBgn0267978

(Bejarano et al., 2008)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

endocytic recycling

inferred from biological aspect of ancestor with PANTHER:PTN001039242

(assigned by GO_Central )

(Gaudet et al., 2011)

regulation of cytokinesis

inferred from biological aspect of ancestor with PANTHER:PTN001039242

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (8 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

apical cortex

inferred from direct assay

(Singari et al., 2014)

colocalizes_with centrosome

inferred from direct assay

(Emery et al., 2005)

colocalizes_with microtubule organizing center

inferred from direct assay

(Riggs et al., 2007)

perinuclear region of cytoplasm

inferred from direct assay

(Giansanti et al., 2007)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

centrosome

traceable author statement

(Tepass et al., 2001)

colocalizes_with centrosome

inferred from biological aspect of ancestor with PANTHER:PTN001039242

(assigned by GO_Central )

(Gaudet et al., 2011)

cleavage furrow

inferred from biological aspect of ancestor with PANTHER:PTN001039242

(assigned by GO_Central )

(Gaudet et al., 2011)

endocytic vesicle

inferred from biological aspect of ancestor with PANTHER:PTN001039242

(assigned by GO_Central )

(Gaudet et al., 2011)

midbody

inferred from biological aspect of ancestor with PANTHER:PTN001039242

(assigned by GO_Central )

(Gaudet et al., 2011)

recycling endosome membrane

inferred from biological aspect of ancestor with PANTHER:PTN001039242

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

central brain anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

cardiogenic mesoderm

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

dorsal vessel primordium

Comment: reported as dorsal vessel specific anlage

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

stomatogastric nervous system primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic/larval dorsal vessel

(Fisher et al., 2012)

embryonic/larval salivary gland body

(Fisher et al., 2012)

embryonic stage 11 -- 13

cardiogenic mesoderm

(Ahmad et al., 2012)

embryonic stage 16 -- 17

embryonic/larval heart

(Ahmad et al., 2012)

embryonic stage

ventral midline

(Wheeler et al., 2009)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 6

Comment: reference states 0-3 hr AEL

(Rothwell et al., 1998)

Additional Descriptive Data

nuf transcripts are detected in 0-3 hr embryos.

(Rothwell et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis & adult stage | male

spermatocyte

Comment: 0-2 days post eclosion

(Polevoy et al., 2009)

oogenesis & adult stage | female

oocyte associated follicle cell | apical

(Khanal et al., 2016)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5

(Rothwell et al., 1998)

embryonic stage 4

(Rothwell et al., 1998)

embryonic stage 1 -- 6

Comment: reference states 0-3 hr AEL

(Rothwell et al., 1998)

Additional Descriptive Data

A major band of 95 kD and minor bands of 82 and 67 kD are detected in Western blots of 0-3 hr embryos. Following phosphatase treatment, only a single 67 kD band is observed. During late syncytial divisions, nuf protein is highly concentrated at the centrosomes during prophase, and is cytoplasmic during other stages of the nuclear cycle. nuf protein is also concentrated at the centrosomes during interphase of nuclear cycle 14, and during cellularization.

(Rothwell et al., 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

apical cortex

inferred from direct assay

(Singari et al., 2014)

colocalizes_with centrosome

inferred from direct assay

(Emery et al., 2005)

colocalizes_with microtubule organizing center

inferred from direct assay

(Riggs et al., 2007)

perinuclear region of cytoplasm

inferred from direct assay

(Giansanti et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\nuf in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\nuf

View exonic expression by tissue for Dmel\nuf

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0013718

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0013718

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG7867-RA;CG32140-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7867

Flygut - An atlas of the Drosophila adult midgut

FBgn0013718

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\nuf

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 35 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of nuf

Transgenic constructs containing regulatory region of nuf

Deletions and Duplications ( 4 )

Disrupted in

Df(3L)BSC801

(Christensen et al., 2009.5.6)

Df(3L)D⁵

(Carpenter, 2001.3.12, Peterson and Petri, 1995)

Df(3L)Exel6121

(Ryder, 2004.4.26)

Duplicated in

Dp(3;4)D^4rv42

(Peterson and Petri, 1995)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | maternal effect | recessive

lethal - all die before end of embryonic stage | maternal effect

nuf¹

lethal - all die before end of embryonic stage | maternal effect (with nuf¹)

nuf^Cy3-23

lethal - all die before end of embryonic stage | maternal effect (with nuf^Cy3-23)

nuf¹

partially lethal - majority die, with Scer\GAL4^pnr-MD237

nuf^GD10800

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

nuf^GD12309

viable, with Scer\GAL4^tin.CΔ4

nuf^GD12309

Sterility

Allele

fertile

Other Phenotypes

Allele

neuroanatomy defective | larval stage, with Scer\GAL4^elav-C155

nuf^EP3077

some die during embryonic stage | maternal effect

nuf¹

some die during embryonic stage | maternal effect (with nuf¹)

nuf^Cy3-23

some die during embryonic stage | maternal effect (with nuf^Cy3-23)

nuf¹

some die during pupal stage, with Scer\GAL4^pnr-MD237

nuf^GD10800

visible, with Scer\GAL4^Bx-MS1096-KE

nuf^BRW

visible, with Scer\GAL4^ey.PH

nuf^BRW

visible, with Scer\GAL4^GMR.PF

nuf^BRW

visible, with Scer\GAL4^sca-537.4

wild-type

nuf^Germ.10

Phenotype manifest in

Allele

actin cytoskeleton | embryonic stage

nuf¹

anterior fascicle & synapse, with Scer\GAL4^elav-C155

nuf^EP3077

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

nuf^EP3077

cleavage furrow

nuf¹

embryo | embryonic cycle 11 | maternal effect

nuf¹

embryo | embryonic cycle 12 | maternal effect

nuf¹

embryo | embryonic cycle 13 | maternal effect

nuf¹

embryo | embryonic cycle 14 | maternal effect

nuf¹

eye, with Scer\GAL4^ey.PH

nuf^BRW

eye, with Scer\GAL4^GMR.PF

nuf^BRW

intersegmental nerve, with Scer\GAL4^elav-C155

nuf^EP3077

macrochaeta

nuf^BG02180

mesothoracic bristle | adult stage | supernumerary, with Scer\GAL4^sca.PU

nuf^EP3324

mitotic telophase | maternal effect

nuf¹

nucleus | embryonic stage

nuf¹

scutellar bristle, with Scer\GAL4^sca-537.4

nuf^EP3339

scutellar bristle | ectopic, with Scer\GAL4^sca-537.4

tormogen cell | supernumerary, with Scer\GAL4^sca-109-68

nuf^EP3324

trichogen cell, with Scer\GAL4^sca-109-68

nuf^EP3324

trichogen cell | ectopic, with Scer\GAL4^sca-537.4

nuf^EP3324

wing, with Scer\GAL4^Bx-MS1096-KE

nuf^BRW

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RAB11FIP4

11 of 15

Yes

Hsap\RAB11FIP3

10 of 15

Yes

Hsap\MIR4724

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rab11fip4

12 of 15

Yes

Mmus\Rab11fip3

10 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Rab11fip4

10 of 13

Yes

Rnor\Rab11fip3

3 of 13

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\rab11fip4

7 of 12

Yes

Xtro\rab11fip3

5 of 12

Yes

Xtro\rab11fip4l

2 of 12

Yes

Xtro\ppfia1

1 of 12

Danio rerio (Zebrafish) (3)

Drer\rab11fip4a

11 of 15

Yes

Drer\rab11fip4b

9 of 15

Yes

Drer\rab11fip3

8 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\rfip-1

12 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190A51 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA23801

Drosophila willistoni

Dwil\GK12678

Drosophila virilis

Dvir\GJ11373

Drosophila mojavensis

Dmoj\GI11698

Drosophila grimshawi

Dgri\GH17096

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150A2E )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17027337

Musca domestica

House fly

Mdoa\17004641

Glossina morsitans

Tsetse fly

Gmor\GMOY007640

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_13216

Mayetiola destructor

Hessian fly

Mdes\Mdes004120

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006776

Anopheles darlingi

American malaria mosquito

Adar\ADAC007189

Anopheles gambiae

Malaria mosquito

Agam\AGAP010445

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015278

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0BB9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA011840

Danaus plexippus

Monarch butterfly

Dple\DPOGS213560-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL010871

Apis florea

Little honeybee

Aflo\2421268372

Apis mellifera

Western honey bee

Amel\GB44363

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13614

Bombus terrestris

Buff-tailed bumblebee

Bter\15209554

Linepithema humile

Argentine ant

Lhum\LH18492

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000d89

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG014545

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_10256

Tribolium castaneum

Red flour beetle

Tcas\TC032404

Rhodnius prolixus

Kissing bug

Rpro\RPRC010233

Cimex lectularius

Bed bug

Clec\CLEC004473

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007157

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_15655

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0B8Y )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR011612

Ixodes scapularis

Black-legged tick

Isca\ISCW016229

Stegodyphus mimosarum

African social velvet spider

Smim\KFM76180.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g10590

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_55126

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0HSY )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_015543gn

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAB11FIP4; RAB11 family interacting protein 4

11 of 15

611999

RAB11FIP3; RAB11 family interacting protein 3

10 of 15

608738

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

nuf

two hybrid

(Hu et al., 2017.6.13)

nuf - 14-3-3ε

pull down, western blot

(Calero-Cuenca et al., 2016)

nuf - CG15728

two hybrid

(Hu et al., 2017.6.13)

nuf - DCTN1-p150

pull down, western blot

(Calero-Cuenca et al., 2016)

nuf - Dhc64C

anti tag coimmunoprecipitation, western blot

(Riggs et al., 2007)

nuf - Dlic

pull down, molecular weight estimation by staining, western blot

(Calero-Cuenca et al., 2016, Otani et al., 2011)

nuf - Gsc

two hybrid

(Hu et al., 2017.6.13)

nuf - IKKε

pull down, western blot, enzymatic study, molecular weight estimation by staining

(Otani et al., 2011)

nuf - Khc

pull down, western blot

(Calero-Cuenca et al., 2016)

nuf - Rab11

pull down, molecular weight estimation by staining, colocalization, fluorescence microscopy, inferred by author, peptide massfingerprinting, anti tag western blot, western blot

(Calero-Cuenca et al., 2016, Gillingham et al., 2014, Singari et al., 2014, Ramel et al., 2013, Otani et al., 2011, Riggs et al., 2003)

nuf - aPKC

anti tag coimmunoprecipitation, peptide massfingerprinting, pull down, western blot, protein kinase assay, autoradiography

(Calero-Cuenca et al., 2016)

nuf - dx

two hybrid

(Hu et al., 2017.6.13)

nuf - polo

pull down, western blot, enzymatic study, autoradiography

(Brose et al., 2017)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

nuf

enhanceable

l(2)gl

(Emery et al., 2005)

nuf

enhanceable

Rab11

(Emery et al., 2005)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

nuf

(Bejarano et al., 2008)

Bx|ap

suppressible

nuf

(Bejarano et al., 2008)

IKKε

suppressible

nuf

(Otani et al., 2011)

l(2)gl

enhanceable

nuf

(Emery et al., 2005)

enhanceable

(Sun et al., 2006)

suppressible

(Gregory et al., 2007)

Rab11

enhanceable

nuf

(Emery et al., 2005)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64907

DroID - A comprehensive database of gene and protein interactions.

FBgn0013718

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

39572

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

VxPx cargo-targeting to cilium

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-42

Cytogenetic map

70D3-70D4

Sequence location

3L:14,190,870..14,233,250 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

70D3-70D4

Limits computationally determined from genome sequence between P{PZ}l(3)70Da⁰²⁴⁰²&P{PZ}btl⁰⁰²⁰⁸ and P{PZ}Mpcp⁰⁰⁵⁶⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

70D3-70D3

(determined by in situ hybridisation)

(Abdelilah-Seyfried et al., 2000)

70D3-70D3

(Rothwell, 1998.1.27, Rothwell et al., 1996)

70-70

(determined by in situ hybridisation)

(Sullivan et al., 1993)

Experimentally Determined Recombination Data

Location

3-42

(FlyBase, 2016)

3-35.8

(Comeron et al., 2012)

(Rothwell et al., 1996)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (27)

Bloomington

56543

w^*; Mi{Hto-WP}nuf^BRW

18168

w¹¹¹⁸; PBac{RB}nuf^e03568

27803

w¹¹¹⁸; Mi{ET1}nuf^MB09772

31381

y¹ v¹; P{TRiP.JF01350}attP2

31493

y¹ v¹; P{TRiP.JF01351}attP2

Kyoto

123480

w¹¹¹⁸; P{RS3}nuf^CB-5139-3

124188

w¹¹¹⁸; P{RS3}nuf^CB-6699-3

125863

w¹¹¹⁸; P{RS5}nuf^5-SZ-3064

VDRC

v104172

P{KK106020}VIE-260B

v109426

P{KK108267}VIE-260B

v28069

w¹¹¹⁸; P{GD12309}v28069

v34417

w¹¹¹⁸; P{GD10800}v34417

More stocks available...

Genomic Clones (34)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (146)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0013718

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0013718

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39572

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Calero-Cuenca et al., 2016, Otani et al., 2011, Rothwell et al., 1998)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: nuf CG7867

(Bayraktaroglu, 1999.11)

Source for database merge of

Source for merge of: nuf CIP-D2 anon-D2

(Perkins, 2001.9.13)

Source for merge of: nuf EP3077

(FlyBase Genome Annotators, 2005, FlyBase, 1996-)

Additional comments

Source for merge of nuf EP3077 was location of EP3077 insertion ( date:020218 ).

(FlyBase Genome Annotators, 2005, FlyBase, 1996-)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

New annotation (CG32140) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

The nuf-mutant phenotype is used to investigate the process by which a furrow is constructed.

(Rothwell et al., 1998)

Protein interacts specifically with csw unique catalytic insert domain.

(Johnson et al., 1997)

Identification: Yeast two hybrid screen for proteins that interact with the csw protein's protein phosphatase domain.

(Denhez et al., 1996)

nuf gene product has a zinc finger motif.

(Peterson and Petri, 1995)

Deficiency analysis localized nuf to 70D2-70D6.

(Sullivan et al., 1993)

Defined in a screen of maternal-effect lethal mutations that cause defects in nuclear movement, organization or morphology during the syncytial embryonic divisions.

(Sullivan et al., 1993)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 113 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39572

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0013718

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0013718

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7867

Flygut - An atlas of the Drosophila adult midgut

FBgn0013718

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39572

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032404

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG33991

modMine - A data warehouse for the modENCODE project

FBgn0013718

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

BioGRID - A database of protein and genetic interactions.

64907

DPiM - Drosophila Protein interaction map

FBgn0013718

DroID - A comprehensive database of gene and protein interactions.

FBgn0013718

DRSC - Results frm RNAi screens

FBgn0013718

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0013718

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG7867-RA;CG32140-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0013718

FlyMine - An integrated database for Drosophila genomics

FBgn0013718

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

39572

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

VxPx cargo-targeting to cilium

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

CG32140

(Giot et al., 2003)

CG33991

(Ramel et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, FlyBase Genome Annotators, 2005)

CG3779

(Ahmad et al., 2012)

CG7867

(Ahmad et al., 2012, Norga et al., 2003, Goldstein and Gunawardena, 2000)

CIP-D2

(Perkins, 2001.9.13)

Cy3-23

(Peterson and Petri, 1995)

(Lorenzen et al., 1998, Johnson et al., 1997, Denhez et al., 1996)

EP3077

FIP3

(van IJzendoorn, 2006)

NUF

(Lecuit, 2004)

Nuf1

(Xu et al., 2011)

Nuf2p

(White and Erickson, 2009)

anon-D2

(Perkins, 2001.9.13)

Name Synonyms

Corkscrew Interacting Protein-D2

(Perkins, 2001.9.13)

Nuclear Fallout

(Thomas and Noselli, 2007)

Nuclear fallout

(Ramel et al., 2013, Fabian and Brill, 2012, Cao et al., 2008, Slack et al., 2007, Polo and Di, 2006, van IJzendoorn, 2006)

Nuclear-fallout

(McCleland and O'Farrell, 2008, Riggs et al., 2007)

nuclear fallout

(Pogson et al., 2014, Singari et al., 2014, Gault et al., 2012, Gould, 2011, Karbowniczek et al., 2010, Gregory et al., 2007, Morozova et al., 2007, Morozova et al., 2006, Burnette et al., 2005, Perkins, 2001.9.13)

nuclear-fallout

(Lecuit, 2004, Scaria et al., 2004, Sullivan et al., 1993)

Secondary FlyBase IDs

FBgn0013768
FBgn0015626
FBgn0024703
FBgn0044464
FBgn0052140
FBgn0053991
FBtr0075731
FBtr0075732
FBtr0075733
FBtr0075734
FBtr0075738
FBpp0075480
FBtr0075739
FBpp0075481

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (144)

Report Sections

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