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Gene Dmel\pnut

General Information
Symbol	Dmel\pnut	Species	D. melanogaster
Name	peanut	Annotation symbol	CG8705
Feature type	protein_coding_gene	FlyBase ID	FBgn0013726
Gene Model Status	Current	Stock availability	9 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	44C2-44C2	Sequence location	2R:4,124,776..4,127,802 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene peanut is referred to in FlyBase by the symbol Dmel\pnut (CG8705, FBgn0013726). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: actin binding; ubiquitin protein ligase binding; microtubule binding; GTPase activity. There is experimental evidence that it is involved in the biological process: apoptotic process; GTP catabolic process; cytokinesis; positive regulation of apoptotic process. 20 alleles are reported. The phenotypes of these alleles are annotated with: photoreceptor cell R7; imaginal ring; embryonic/larval lymph gland; trichogen cell; follicle cell; salivary gland common duct primordium; imaginal disc; dorsal appendage. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Cell division protein GTP binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval midgut, larval hindgut, larval Malpighian tubules, larval/adult salivary gland, larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, adult midgut, adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, inseminated spermatheca, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1627280_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of pnut. Gene sequence location is 2R:4124776..4127802.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 44C2-44C2   Limits computationally determined from genome sequence between P{lacW}Rs1k09514&P{lacW}l(2)k03110k03110 and P{lacW}Vps25k08904&P{lacW}ptck02507
Experimentally Determined Cytological Location
Cytogenetic map Notes References 44C1-44C2   (determined by in situ hybridisation)   (Spradling et al., 1999) 44C1-44C2   (determined by in situ hybridisation)   (Neufeld and Rubin, 1994) 44C1-44C2   (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-   2-60 (Carthew et al., 1994)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\pnut for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) pnut-RA FBtr0088793  NM_057716   2445   539 pnut-RB FBtr0088792  NM_165597   3027   539
Additional Transcript Data & Comments
Reported size (kB)	2.5 (northern blot) (Neufeld and Rubin, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein pnut-PA   FBpp0087870   60.1   539   9.14     NP_477064   AAM68857 pnut-PB   FBpp0087869   60.1   539   9.14     NP_724659   AAF59112
Additional Polypeptide Data & Comments
Reported size (kDa)	539 (aa); 60 (kD observed); 60 (kD predicted) (Neufeld and Rubin, 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Cell division protein GTP binding (IPR000038) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201950   AA202231   AA202413   AA202982   AA246222   AA263331   AA263475   AA264431   AA390672   AA392264   AA438431   AA438846   AA439071   AA439495   AA439857   AA440640   AA440940   AA441045   AA536380   AA538734   AA538940   AA539633   AA540124   AA541089   AA694740   AA735110   AA802288   AA816379   AA816884   AA940717   AA942353   AA948821   AA949029   AA951223   AA951299   AA951769   AA978766   AA979448   AE013599 AAF59112   AE013599 AAM68857   AC008370   AI061672   AI114086   AI124305   AI135107   AI135188   AI135219   AI257901   AI260082   AI293244   AI295662   AI295755   AI297010   AI297017   AI388740   AI388985   AI389063   AI456512   AI456852   AI456980   AI511835   AI513033   AI513135   AI513885   AI514422   AI515558   AI516338   AI516552   AI516713   AI519580   AI519873   AI519990   AI520603   AI531276   AI531443   AI532571   AI533416   AI542116   AI544020   AQ073270   AW943371   AY058663 AAL13892   BG636733   BH840394   BH840409   BH840410   BI161880   BI161881   BI162165   BI162166   BI162197   BI162198   BI164060   BI165122   BI165604   BI165817   BI166687   BI169443   BI170855   BI170946   BI171116   BI172611   BI213533   BI215390   BI229454   BI229456   BI229468   BI229492   BI232618   BI233143   BI234305   BI234983   BI237055   BI238361   BI239404   BI239427   BI239637   BI242075   BI351994   BI353316   BI353583   BI354717   BI366481   BI369324   BI369443   BI369599   BI370293   BI370460   BI371445   BI372209   BI372750   BI374377   BI374925   BI374951   BI486700   BI564450   BI567738   BI570849   BI571594   BI576950   BI577329   BI577586   BI579313   BI583161   BI588769   BI590906   BI604254   BI604447   BI621996   BI629042   BI633649   BI636673   BI636711   BI640177   CA806747   CK131799   CK660567   CL705913   CO156762   CO177386   CO179295   CO268270   CO270795   CO274726   CO278140   CO305004   CO306171   CO306310   CO306319   CO309962   CO311156   CO320446   CO321444   CO336690   CO338057   CZ469413   CZ469459   CZ480290   EC069670   EC080417   EC085881   EC195907   EC197104   EC200844   EC203263   EC203501   EC213530   EC214626   EC221479   EC226216   EC226356   EC227583   EC244004   EC247917   EC258896   EC264023   EC265254   EC266023   EL873772   EL873773   EL883026   EV578772   EV583841   GH942632   GH943716   GH944159   GH944206   GH946329   GH947082   GH948567   GH948806   GH951539   GH951861   GH952244   GH953202   GH953267   GH954389   GH955709   GH955836   GH956416   GH957830   GH958535   GH959246   GH959580   GH959696   GH961044   GH962974   GH963302   GH963873   GH965449   GH965909   GH970465   GH970699   GH970806   GH970860   GH971131   GH971562   GH972130   GH972265   GH972903   GH973137   GH980244   GH980705   GH980769   GH983330   GH984717   U08103 AAA19603
UniProtKB/Swiss-Prot	P40797
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
expression microarray Stage Tissue/Position (including subcellular localization) Reference adult stage | female     (Arbeitman et al., 2004)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   central nervous system   (Neufeld and Rubin, 1994) third instar larval stage   photoreceptor cell | presumptive   (Neufeld and Rubin, 1994) adult stage   follicle cell | basal   (Shih et al., 2002)
Additional Descriptive Data
	pnut protein is observed in the plasma membranes of interphase cells at low intensity. In anaphase, membrane staining increases at the newly forming cleavage furrow. The intensity of the staining increases at the furrow as it progresses inward. Following mitosis, staining persists well into interphase at the intercellular bridge connecting daughter cells. (Neufeld and Rubin, 1994)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008) plasma membrane inferred from direct assay (Huijbregts et al., 2009) septin complex inferred from direct assay (Huijbregts et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval midgut, larval hindgut, larval Malpighian tubules, larval/adult salivary gland, larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, adult midgut, adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, inseminated spermatheca, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1062.875 Larval Midgut    501.7 Larval Hindgut    663 Larval Malpighian Tubules    968.8 Larval Fat Body    160.9 Larval Salivary Gland    755.3 Larval Trachea    793.8 Larval Carcass    464.45 Adult Head    344.1 Adult Eye    161.35 Adult Brain    786.6 Adult Thoracic-Abdominal Ganglion    767.7 Adult Crop    180.2 Adult Midgut    329.1 Adult Hindgut    248.5 Adult Malpighian Tubules    281.1 Adult Fat Body    103.3 Adult Salivary Gland    551.3 Adult Heart    357.775 Adult VirginFemale Spermatheca    91.2 Adult InseminatedFemale Spermatheca    117.5 Adult Ovary    1009.5 Adult Testis    123.8 Adult Male Accessory Gland    207.8 Adult Carcass    174.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1062.875) Larval Midgut  501.7 Larval Hindgut  (663) Larval Malpighian Tubules  (968.8) Larval Fat Body    160.9 Larval Salivary Gland  (755.3) Larval Trachea  (793.8) Larval Carcass    464.45 Adult Head    344.1 Adult Eye    161.35 Adult Brain  (786.6) Adult Thoracic-Abdominal Ganglion  (767.7) Adult Crop    180.2 Adult Midgut    329.1 Adult Hindgut    248.5 Adult Malpighian Tubules    281.1 Adult Fat Body    103.3 Adult Salivary Gland  (551.3) Adult Heart    357.775 Adult VirginFemale Spermatheca    91.2 Adult InseminatedFemale Spermatheca    117.5 Adult Ovary  (1009.5) Adult Testis    123.8 Adult Male Accessory Gland    207.8 Adult Carcass    174.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1062.875 Larval Midgut    501.7 Larval Hindgut    663 Larval Malpighian Tubules    968.8 Larval Fat Body    160.9 Larval Salivary Gland    755.3 Larval Trachea    793.8 Larval Carcass    464.45 Adult Head    344.1 Adult Eye    161.35 Adult Brain    786.6 Adult Thoracic-Abdominal Ganglion    767.7 Adult Crop    180.2 Adult Midgut    329.1 Adult Hindgut    248.5 Adult Malpighian Tubules    281.1 Adult Fat Body    103.3 Adult Salivary Gland    551.3 Adult Heart    357.775 Adult VirginFemale Spermatheca    91.2 Adult InseminatedFemale Spermatheca    117.5 Adult Ovary  1009.5 Adult Testis    123.8 Adult Male Accessory Gland    207.8 Adult Carcass    174.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1062.875 Larval Midgut    501.7 Larval Hindgut    663 Larval Malpighian Tubules    968.8 Larval Fat Body    160.9 Larval Salivary Gland    755.3 Larval Trachea    793.8 Larval Carcass    464.45 Adult Head    344.1 Adult Eye    161.35 Adult Brain    786.6 Adult Thoracic-Abdominal Ganglion    767.7 Adult Crop    180.2 Adult Midgut    329.1 Adult Hindgut    248.5 Adult Malpighian Tubules    281.1 Adult Fat Body    103.3 Adult Salivary Gland    551.3 Adult Heart    357.775 Adult VirginFemale Spermatheca    91.2 Adult InseminatedFemale Spermatheca    117.5 Adult Ovary    1009.5 Adult Testis    123.8 Adult Male Accessory Gland    207.8 Adult Carcass    174.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1062.875 Larval Midgut    501.7 Larval Hindgut    663 Larval Malpighian Tubules    968.8 Larval Fat Body    160.9 Larval Salivary Gland    755.3 Larval Trachea    793.8 Larval Carcass    464.45 Adult Head    344.1 Adult Eye    161.35 Adult Brain    786.6 Adult Thoracic-Abdominal Ganglion    767.7 Adult Crop    180.2 Adult Midgut    329.1 Adult Hindgut    248.5 Adult Malpighian Tubules    281.1 Adult Fat Body    103.3 Adult Salivary Gland    551.3 Adult Heart    357.775 Adult VirginFemale Spermatheca    91.2 Adult InseminatedFemale Spermatheca    117.5 Adult Ovary    1009.5 Adult Testis    123.8 Adult Male Accessory Gland    207.8 Adult Carcass    174.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1062.875) Larval Midgut  501.7 Larval Hindgut  663 Larval Malpighian Tubules  (968.8) Larval Fat Body    160.9 Larval Salivary Gland  755.3 Larval Trachea  793.8 Larval Carcass    464.45 Adult Head    344.1 Adult Eye    161.35 Adult Brain  786.6 Adult Thoracic-Abdominal Ganglion  767.7 Adult Crop    180.2 Adult Midgut    329.1 Adult Hindgut    248.5 Adult Malpighian Tubules    281.1 Adult Fat Body    103.3 Adult Salivary Gland  551.3 Adult Heart    357.775 Adult VirginFemale Spermatheca    91.2 Adult InseminatedFemale Spermatheca    117.5 Adult Ovary  (1009.5) Adult Testis    123.8 Adult Male Accessory Gland    207.8 Adult Carcass    174.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1062.875 Larval Midgut    501.7 Larval Hindgut    663 Larval Malpighian Tubules    968.8 Larval Fat Body    160.9 Larval Salivary Gland    755.3 Larval Trachea    793.8 Larval Carcass    464.45 Adult Head    344.1 Adult Eye    161.35 Adult Brain    786.6 Adult Thoracic-Abdominal Ganglion    767.7 Adult Crop    180.2 Adult Midgut    329.1 Adult Hindgut    248.5 Adult Malpighian Tubules    281.1 Adult Fat Body    103.3 Adult Salivary Gland    551.3 Adult Heart    357.775 Adult VirginFemale Spermatheca    91.2 Adult InseminatedFemale Spermatheca    117.5 Adult Ovary  1009.5 Adult Testis    123.8 Adult Male Accessory Gland    207.8 Adult Carcass    174.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1062.875 Larval Midgut    501.7 Larval Hindgut    663 Larval Malpighian Tubules    968.8 Larval Fat Body    160.9 Larval Salivary Gland    755.3 Larval Trachea    793.8 Larval Carcass    464.45 Adult Head    344.1 Adult Eye    161.35 Adult Brain    786.6 Adult Thoracic-Abdominal Ganglion    767.7 Adult Crop    180.2 Adult Midgut    329.1 Adult Hindgut    248.5 Adult Malpighian Tubules    281.1 Adult Fat Body    103.3 Adult Salivary Gland    551.3 Adult Heart    357.775 Adult VirginFemale Spermatheca    91.2 Adult InseminatedFemale Spermatheca    117.5 Adult Ovary    1009.5 Adult Testis    123.8 Adult Male Accessory Gland    207.8 Adult Carcass    174.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    344.1   NA  Eye    161.35   NA  Brain    786.6   1062.875  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    767.7   NA  Crop    180.2   501.7  Midgut    329.1   663  Hindgut    248.5   968.8  Malpighian Tubules    281.1   160.9  Fat Body    103.3   755.3  Salivary Gland    551.3   NA  Heart    357.775   793.8  Trachea    NA   NA  VirginFemale Spermatheca    91.2   NA  InseminatedFemale Spermatheca    117.5   NA  Ovary    1009.5   NA  Testis    123.8   NA  Male Accessory Gland    207.8   464.45  Carcass    174.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    69 central nervous system, larvae L3    153 central nervous system, pupae P8    160 head, virgin 1-day female    29 head, virgin 4-day female    29 head, virgin 20-day female    16 head, mated 1-day female    33 head, mated 4-day female    30 head, mated 20-day female    18 head, mated 1-day male    43 head, mated 4-day male    50 head, mated 20-day male    47 salivary gland, larvae L3 wandering    14 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    84 digestive system, 1-day adult    26 digestive system, 4-day adult    34 digestive system, 20-day adult    34 fat body, larvae L3 wandering    8 fat body, white prepupae    30 fat body, pupae P8    79 carcass, larvae L3 wandering    98 carcass, 1-day adult    15 carcass, 4-day adult    20 carcass, 20-day adult    15 ovary, virgin 4-day female    126 ovary, mated 4-day female    147 testis, mated 4-day male    22 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (69) central nervous system, larvae L3  (153) central nervous system, pupae P8  (160) head, virgin 1-day female  (29) head, virgin 4-day female  (29) head, virgin 20-day female    16 head, mated 1-day female  (33) head, mated 4-day female  (30) head, mated 20-day female    18 head, mated 1-day male  (43) head, mated 4-day male  (50) head, mated 20-day male  (47) salivary gland, larvae L3 wandering    14 salivary gland, white prepupae  (57) digestive system, larvae L3 wandering  (84) digestive system, 1-day adult  26 digestive system, 4-day adult  (34) digestive system, 20-day adult  (34) fat body, larvae L3 wandering    8 fat body, white prepupae  (30) fat body, pupae P8  (79) carcass, larvae L3 wandering  (98) carcass, 1-day adult    15 carcass, 4-day adult    20 carcass, 20-day adult    15 ovary, virgin 4-day female  (126) ovary, mated 4-day female  (147) testis, mated 4-day male    22 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    69 central nervous system, larvae L3  (153) central nervous system, pupae P8  (160) head, virgin 1-day female    29 head, virgin 4-day female    29 head, virgin 20-day female    16 head, mated 1-day female    33 head, mated 4-day female    30 head, mated 20-day female    18 head, mated 1-day male    43 head, mated 4-day male    50 head, mated 20-day male    47 salivary gland, larvae L3 wandering    14 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    84 digestive system, 1-day adult    26 digestive system, 4-day adult    34 digestive system, 20-day adult    34 fat body, larvae L3 wandering    8 fat body, white prepupae    30 fat body, pupae P8    79 carcass, larvae L3 wandering    98 carcass, 1-day adult    15 carcass, 4-day adult    20 carcass, 20-day adult    15 ovary, virgin 4-day female  (126) ovary, mated 4-day female  (147) testis, mated 4-day male    22 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    69 central nervous system, larvae L3    153 central nervous system, pupae P8    160 head, virgin 1-day female    29 head, virgin 4-day female    29 head, virgin 20-day female    16 head, mated 1-day female    33 head, mated 4-day female    30 head, mated 20-day female    18 head, mated 1-day male    43 head, mated 4-day male    50 head, mated 20-day male    47 salivary gland, larvae L3 wandering    14 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    84 digestive system, 1-day adult    26 digestive system, 4-day adult    34 digestive system, 20-day adult    34 fat body, larvae L3 wandering    8 fat body, white prepupae    30 fat body, pupae P8    79 carcass, larvae L3 wandering    98 carcass, 1-day adult    15 carcass, 4-day adult    20 carcass, 20-day adult    15 ovary, virgin 4-day female    126 ovary, mated 4-day female    147 testis, mated 4-day male    22 accessory gland, mated 4-day male    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    69 central nervous system, larvae L3    153 central nervous system, pupae P8    160 head, virgin 1-day female    29 head, virgin 4-day female    29 head, virgin 20-day female    16 head, mated 1-day female    33 head, mated 4-day female    30 head, mated 20-day female    18 head, mated 1-day male    43 head, mated 4-day male    50 head, mated 20-day male    47 salivary gland, larvae L3 wandering    14 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    84 digestive system, 1-day adult    26 digestive system, 4-day adult    34 digestive system, 20-day adult    34 fat body, larvae L3 wandering    8 fat body, white prepupae    30 fat body, pupae P8    79 carcass, larvae L3 wandering    98 carcass, 1-day adult    15 carcass, 4-day adult    20 carcass, 20-day adult    15 ovary, virgin 4-day female    126 ovary, mated 4-day female    147 testis, mated 4-day male    22 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (69) central nervous system, larvae L3  (153) central nervous system, pupae P8  (160) head, virgin 1-day female  29 head, virgin 4-day female  29 head, virgin 20-day female    16 head, mated 1-day female  (33) head, mated 4-day female  30 head, mated 20-day female    18 head, mated 1-day male  (43) head, mated 4-day male  (50) head, mated 20-day male  (47) salivary gland, larvae L3 wandering    14 salivary gland, white prepupae  (57) digestive system, larvae L3 wandering  (84) digestive system, 1-day adult  26 digestive system, 4-day adult  (34) digestive system, 20-day adult  (34) fat body, larvae L3 wandering    8 fat body, white prepupae  30 fat body, pupae P8  (79) carcass, larvae L3 wandering  (98) carcass, 1-day adult    15 carcass, 4-day adult    20 carcass, 20-day adult    15 ovary, virgin 4-day female  (126) ovary, mated 4-day female  (147) testis, mated 4-day male    22 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    69 central nervous system, larvae L3  (153) central nervous system, pupae P8  (160) head, virgin 1-day female    29 head, virgin 4-day female    29 head, virgin 20-day female    16 head, mated 1-day female    33 head, mated 4-day female    30 head, mated 20-day female    18 head, mated 1-day male    43 head, mated 4-day male    50 head, mated 20-day male    47 salivary gland, larvae L3 wandering    14 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    84 digestive system, 1-day adult    26 digestive system, 4-day adult    34 digestive system, 20-day adult    34 fat body, larvae L3 wandering    8 fat body, white prepupae    30 fat body, pupae P8    79 carcass, larvae L3 wandering    98 carcass, 1-day adult    15 carcass, 4-day adult    20 carcass, 20-day adult    15 ovary, virgin 4-day female  126 ovary, mated 4-day female  (147) testis, mated 4-day male    22 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    69 central nervous system, larvae L3    153 central nervous system, pupae P8    160 head, virgin 1-day female    29 head, virgin 4-day female    29 head, virgin 20-day female    16 head, mated 1-day female    33 head, mated 4-day female    30 head, mated 20-day female    18 head, mated 1-day male    43 head, mated 4-day male    50 head, mated 20-day male    47 salivary gland, larvae L3 wandering    14 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    84 digestive system, 1-day adult    26 digestive system, 4-day adult    34 digestive system, 20-day adult    34 fat body, larvae L3 wandering    8 fat body, white prepupae    30 fat body, pupae P8    79 carcass, larvae L3 wandering    98 carcass, 1-day adult    15 carcass, 4-day adult    20 carcass, 20-day adult    15 ovary, virgin 4-day female    126 ovary, mated 4-day female    147 testis, mated 4-day male    22 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    107 embryo 02-04hr    188 embryo 04-06hr    161 embryo 06-08hr    173 embryo 08-10hr    146 embryo 10-12hr    129 embryo 12-14hr    95 embryo 14-16hr    96 embryo 16-18hr    65 embryo 18-20hr    41 embryo 20-22hr    58 embryo 22-24hr    52 larva L1    21 larva L2    29 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    63 white prepupae new    76 white prepupae 12hr    62 white prepupae 24hr    66 pupae 2d postWPP    52 pupae 3d postWPP    41 pupae 4d postWPP    14 adult male 01day    15 adult male 05day    13 adult male 30day    13 adult female 01day    41 adult female 05day    62 adult female 30day    66 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (107) embryo 02-04hr  (188) embryo 04-06hr  (161) embryo 06-08hr  (173) embryo 08-10hr  (146) embryo 10-12hr  (129) embryo 12-14hr  (95) embryo 14-16hr  (96) embryo 16-18hr  (65) embryo 18-20hr  (41) embryo 20-22hr  (58) embryo 22-24hr  (52) larva L1    21 larva L2  (29) larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6  (45) larva L3 puffstage 7-9  (63) white prepupae new  (76) white prepupae 12hr  (62) white prepupae 24hr  (66) pupae 2d postWPP  (52) pupae 3d postWPP  (41) pupae 4d postWPP    14 adult male 01day    15 adult male 05day    13 adult male 30day    13 adult female 01day  (41) adult female 05day  (62) adult female 30day  (66) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  107 embryo 02-04hr  (188) embryo 04-06hr  (161) embryo 06-08hr  (173) embryo 08-10hr  (146) embryo 10-12hr  (129) embryo 12-14hr    95 embryo 14-16hr    96 embryo 16-18hr    65 embryo 18-20hr    41 embryo 20-22hr    58 embryo 22-24hr    52 larva L1    21 larva L2    29 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    63 white prepupae new    76 white prepupae 12hr    62 white prepupae 24hr    66 pupae 2d postWPP    52 pupae 3d postWPP    41 pupae 4d postWPP    14 adult male 01day    15 adult male 05day    13 adult male 30day    13 adult female 01day    41 adult female 05day    62 adult female 30day    66 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    107 embryo 02-04hr    188 embryo 04-06hr    161 embryo 06-08hr    173 embryo 08-10hr    146 embryo 10-12hr    129 embryo 12-14hr    95 embryo 14-16hr    96 embryo 16-18hr    65 embryo 18-20hr    41 embryo 20-22hr    58 embryo 22-24hr    52 larva L1    21 larva L2    29 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    63 white prepupae new    76 white prepupae 12hr    62 white prepupae 24hr    66 pupae 2d postWPP    52 pupae 3d postWPP    41 pupae 4d postWPP    14 adult male 01day    15 adult male 05day    13 adult male 30day    13 adult female 01day    41 adult female 05day    62 adult female 30day    66 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    107 embryo 02-04hr    188 embryo 04-06hr    161 embryo 06-08hr    173 embryo 08-10hr    146 embryo 10-12hr    129 embryo 12-14hr    95 embryo 14-16hr    96 embryo 16-18hr    65 embryo 18-20hr    41 embryo 20-22hr    58 embryo 22-24hr    52 larva L1    21 larva L2    29 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    63 white prepupae new    76 white prepupae 12hr    62 white prepupae 24hr    66 pupae 2d postWPP    52 pupae 3d postWPP    41 pupae 4d postWPP    14 adult male 01day    15 adult male 05day    13 adult male 30day    13 adult female 01day    41 adult female 05day    62 adult female 30day    66 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (107) embryo 02-04hr  (188) embryo 04-06hr  (161) embryo 06-08hr  (173) embryo 08-10hr  (146) embryo 10-12hr  (129) embryo 12-14hr  (95) embryo 14-16hr  (96) embryo 16-18hr  (65) embryo 18-20hr  (41) embryo 20-22hr  (58) embryo 22-24hr  (52) larva L1    21 larva L2  29 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6  (45) larva L3 puffstage 7-9  (63) white prepupae new  (76) white prepupae 12hr  (62) white prepupae 24hr  (66) pupae 2d postWPP  (52) pupae 3d postWPP  (41) pupae 4d postWPP    14 adult male 01day    15 adult male 05day    13 adult male 30day    13 adult female 01day  (41) adult female 05day  (62) adult female 30day  (66) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  107 embryo 02-04hr  (188) embryo 04-06hr  (161) embryo 06-08hr  (173) embryo 08-10hr  (146) embryo 10-12hr  129 embryo 12-14hr    95 embryo 14-16hr    96 embryo 16-18hr    65 embryo 18-20hr    41 embryo 20-22hr    58 embryo 22-24hr    52 larva L1    21 larva L2    29 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    63 white prepupae new    76 white prepupae 12hr    62 white prepupae 24hr    66 pupae 2d postWPP    52 pupae 3d postWPP    41 pupae 4d postWPP    14 adult male 01day    15 adult male 05day    13 adult male 30day    13 adult female 01day    41 adult female 05day    62 adult female 30day    66 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    107 embryo 02-04hr    188 embryo 04-06hr    161 embryo 06-08hr    173 embryo 08-10hr    146 embryo 10-12hr    129 embryo 12-14hr    95 embryo 14-16hr    96 embryo 16-18hr    65 embryo 18-20hr    41 embryo 20-22hr    58 embryo 22-24hr    52 larva L1    21 larva L2    29 larva L3 12hr old    15 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    45 larva L3 puffstage 7-9    63 white prepupae new    76 white prepupae 12hr    62 white prepupae 24hr    66 pupae 2d postWPP    52 pupae 3d postWPP    41 pupae 4d postWPP    14 adult male 01day    15 adult male 05day    13 adult male 30day    13 adult female 01day    41 adult female 05day    62 adult female 30day    66 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    177 Schneider line 2 Sg4    182 embryonic 1182-4H    175 embryonic GM2    141 embryonic Kc167    105 embryonic S1    164 embryonic S3    110 leg disc CME L1    196 wing disc CME-W2    119 wing disc ML-DmD8    198 wing disc ML-DmD9    159 wing disc ML-DmD16-c3    396 wing disc ML-DmD21    356 wing disc ML-DmD32    158 haltere disc ML-DmD17-c3    217 eye-antennal disc ML-DmD11    99 antennal disc ML-DmD20-c5    165 mixed discs ML-DmD4-c1    360 CNS ML-DmBG1-c1    440 CNS ML-DmBG2-c2    265 tumorous blood cells mbn2    100 ovary fGS/OSS    65 ovary OSC    141 ovary OSS    141 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (177) Schneider line 2 Sg4  (182) embryonic 1182-4H  (175) embryonic GM2  (141) embryonic Kc167  (105) embryonic S1  (164) embryonic S3  (110) leg disc CME L1  (196) wing disc CME-W2  (119) wing disc ML-DmD8  (198) wing disc ML-DmD9  (159) wing disc ML-DmD16-c3  (396) wing disc ML-DmD21  (356) wing disc ML-DmD32  (158) haltere disc ML-DmD17-c3  (217) eye-antennal disc ML-DmD11  (99) antennal disc ML-DmD20-c5  (165) mixed discs ML-DmD4-c1  (360) CNS ML-DmBG1-c1  (440) CNS ML-DmBG2-c2  (265) tumorous blood cells mbn2  (100) ovary fGS/OSS  (65) ovary OSC  (141) ovary OSS  (141) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (177) Schneider line 2 Sg4  (182) embryonic 1182-4H  (175) embryonic GM2  (141) embryonic Kc167  105 embryonic S1  (164) embryonic S3  (110) leg disc CME L1  (196) wing disc CME-W2  (119) wing disc ML-DmD8  (198) wing disc ML-DmD9  (159) wing disc ML-DmD16-c3  (396) wing disc ML-DmD21  (356) wing disc ML-DmD32  (158) haltere disc ML-DmD17-c3  (217) eye-antennal disc ML-DmD11    99 antennal disc ML-DmD20-c5  (165) mixed discs ML-DmD4-c1  (360) CNS ML-DmBG1-c1  (440) CNS ML-DmBG2-c2  (265) tumorous blood cells mbn2    100 ovary fGS/OSS    65 ovary OSC  (141) ovary OSS  (141) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    177 Schneider line 2 Sg4    182 embryonic 1182-4H    175 embryonic GM2    141 embryonic Kc167    105 embryonic S1    164 embryonic S3    110 leg disc CME L1    196 wing disc CME-W2    119 wing disc ML-DmD8    198 wing disc ML-DmD9    159 wing disc ML-DmD16-c3    396 wing disc ML-DmD21    356 wing disc ML-DmD32    158 haltere disc ML-DmD17-c3    217 eye-antennal disc ML-DmD11    99 antennal disc ML-DmD20-c5    165 mixed discs ML-DmD4-c1    360 CNS ML-DmBG1-c1    440 CNS ML-DmBG2-c2    265 tumorous blood cells mbn2    100 ovary fGS/OSS    65 ovary OSC    141 ovary OSS    141 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    177 Schneider line 2 Sg4    182 embryonic 1182-4H    175 embryonic GM2    141 embryonic Kc167    105 embryonic S1    164 embryonic S3    110 leg disc CME L1    196 wing disc CME-W2    119 wing disc ML-DmD8    198 wing disc ML-DmD9    159 wing disc ML-DmD16-c3    396 wing disc ML-DmD21    356 wing disc ML-DmD32    158 haltere disc ML-DmD17-c3    217 eye-antennal disc ML-DmD11    99 antennal disc ML-DmD20-c5    165 mixed discs ML-DmD4-c1    360 CNS ML-DmBG1-c1    440 CNS ML-DmBG2-c2    265 tumorous blood cells mbn2    100 ovary fGS/OSS    65 ovary OSC    141 ovary OSS    141 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (177) Schneider line 2 Sg4  (182) embryonic 1182-4H  (175) embryonic GM2  (141) embryonic Kc167  (105) embryonic S1  (164) embryonic S3  (110) leg disc CME L1  (196) wing disc CME-W2  (119) wing disc ML-DmD8  (198) wing disc ML-DmD9  (159) wing disc ML-DmD16-c3  (396) wing disc ML-DmD21  (356) wing disc ML-DmD32  (158) haltere disc ML-DmD17-c3  (217) eye-antennal disc ML-DmD11  (99) antennal disc ML-DmD20-c5  (165) mixed discs ML-DmD4-c1  (360) CNS ML-DmBG1-c1  (440) CNS ML-DmBG2-c2  (265) tumorous blood cells mbn2  (100) ovary fGS/OSS  (65) ovary OSC  (141) ovary OSS  (141) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (177) Schneider line 2 Sg4  (182) embryonic 1182-4H  (175) embryonic GM2  141 embryonic Kc167  105 embryonic S1  (164) embryonic S3  110 leg disc CME L1  (196) wing disc CME-W2  119 wing disc ML-DmD8  (198) wing disc ML-DmD9  (159) wing disc ML-DmD16-c3  (396) wing disc ML-DmD21  (356) wing disc ML-DmD32  (158) haltere disc ML-DmD17-c3  (217) eye-antennal disc ML-DmD11    99 antennal disc ML-DmD20-c5  (165) mixed discs ML-DmD4-c1  (360) CNS ML-DmBG1-c1  (440) CNS ML-DmBG2-c2  (265) tumorous blood cells mbn2    100 ovary fGS/OSS    65 ovary OSC  141 ovary OSS  141 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    177 Schneider line 2 Sg4    182 embryonic 1182-4H    175 embryonic GM2    141 embryonic Kc167    105 embryonic S1    164 embryonic S3    110 leg disc CME L1    196 wing disc CME-W2    119 wing disc ML-DmD8    198 wing disc ML-DmD9    159 wing disc ML-DmD16-c3    396 wing disc ML-DmD21    356 wing disc ML-DmD32    158 haltere disc ML-DmD17-c3    217 eye-antennal disc ML-DmD11    99 antennal disc ML-DmD20-c5    165 mixed discs ML-DmD4-c1    360 CNS ML-DmBG1-c1    440 CNS ML-DmBG2-c2    265 tumorous blood cells mbn2    100 ovary fGS/OSS    65 ovary OSC    141 ovary OSS    141 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    93 cold shock, 4-day adult    66 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    30 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    47 Cadmium 100 mM 48 hrs, 4-day adult    112 Copper 0.5 mM 12 hrs, larvae L3    54 Copper 15 mM 48 hrs, 4-day adult    104 Zinc 5 mM 12 hrs, larvae L3    41 Zinc 4.5 mM 48 hrs, 4-day adult    52 Ethanol 2.5% 3 hrs, larvae L3    80 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    101 Caffeine 25 mg/ml 48 hrs, 4-day adult    80 Paraquat 5 mM 48 hrs, 4-day adult    70 Paraquat 10 mM 48 hrs, 4-day adult    63 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (93) cold shock, 4-day adult  (66) heat shock, 4-day adult  (49) Cadmium 50 mM 6 hrs, larvae L3  (30) Cadmium 50 mM 12 hrs, larvae L3  (27) Cadmium 50 mM 48 hrs, 4-day adult  (47) Cadmium 100 mM 48 hrs, 4-day adult  (112) Copper 0.5 mM 12 hrs, larvae L3  (54) Copper 15 mM 48 hrs, 4-day adult  (104) Zinc 5 mM 12 hrs, larvae L3  (41) Zinc 4.5 mM 48 hrs, 4-day adult  (52) Ethanol 2.5% 3 hrs, larvae L3  (80) Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (101) Caffeine 25 mg/ml 48 hrs, 4-day adult  (80) Paraquat 5 mM 48 hrs, 4-day adult  (70) Paraquat 10 mM 48 hrs, 4-day adult  (63) Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    93 cold shock, 4-day adult    66 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    30 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    47 Cadmium 100 mM 48 hrs, 4-day adult  (112) Copper 0.5 mM 12 hrs, larvae L3    54 Copper 15 mM 48 hrs, 4-day adult  104 Zinc 5 mM 12 hrs, larvae L3    41 Zinc 4.5 mM 48 hrs, 4-day adult    52 Ethanol 2.5% 3 hrs, larvae L3    80 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  101 Caffeine 25 mg/ml 48 hrs, 4-day adult    80 Paraquat 5 mM 48 hrs, 4-day adult    70 Paraquat 10 mM 48 hrs, 4-day adult    63 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    93 cold shock, 4-day adult    66 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    30 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    47 Cadmium 100 mM 48 hrs, 4-day adult    112 Copper 0.5 mM 12 hrs, larvae L3    54 Copper 15 mM 48 hrs, 4-day adult    104 Zinc 5 mM 12 hrs, larvae L3    41 Zinc 4.5 mM 48 hrs, 4-day adult    52 Ethanol 2.5% 3 hrs, larvae L3    80 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    101 Caffeine 25 mg/ml 48 hrs, 4-day adult    80 Paraquat 5 mM 48 hrs, 4-day adult    70 Paraquat 10 mM 48 hrs, 4-day adult    63 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    93 cold shock, 4-day adult    66 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    30 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    47 Cadmium 100 mM 48 hrs, 4-day adult    112 Copper 0.5 mM 12 hrs, larvae L3    54 Copper 15 mM 48 hrs, 4-day adult    104 Zinc 5 mM 12 hrs, larvae L3    41 Zinc 4.5 mM 48 hrs, 4-day adult    52 Ethanol 2.5% 3 hrs, larvae L3    80 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    101 Caffeine 25 mg/ml 48 hrs, 4-day adult    80 Paraquat 5 mM 48 hrs, 4-day adult    70 Paraquat 10 mM 48 hrs, 4-day adult    63 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (93) cold shock, 4-day adult  (66) heat shock, 4-day adult  (49) Cadmium 50 mM 6 hrs, larvae L3  30 Cadmium 50 mM 12 hrs, larvae L3  27 Cadmium 50 mM 48 hrs, 4-day adult  (47) Cadmium 100 mM 48 hrs, 4-day adult  (112) Copper 0.5 mM 12 hrs, larvae L3  (54) Copper 15 mM 48 hrs, 4-day adult  (104) Zinc 5 mM 12 hrs, larvae L3  (41) Zinc 4.5 mM 48 hrs, 4-day adult  (52) Ethanol 2.5% 3 hrs, larvae L3  (80) Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (101) Caffeine 25 mg/ml 48 hrs, 4-day adult  (80) Paraquat 5 mM 48 hrs, 4-day adult  (70) Paraquat 10 mM 48 hrs, 4-day adult  (63) Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    93 cold shock, 4-day adult    66 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    30 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    47 Cadmium 100 mM 48 hrs, 4-day adult  112 Copper 0.5 mM 12 hrs, larvae L3    54 Copper 15 mM 48 hrs, 4-day adult  104 Zinc 5 mM 12 hrs, larvae L3    41 Zinc 4.5 mM 48 hrs, 4-day adult    52 Ethanol 2.5% 3 hrs, larvae L3    80 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  101 Caffeine 25 mg/ml 48 hrs, 4-day adult    80 Paraquat 5 mM 48 hrs, 4-day adult    70 Paraquat 10 mM 48 hrs, 4-day adult    63 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    93 cold shock, 4-day adult    66 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    30 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    47 Cadmium 100 mM 48 hrs, 4-day adult    112 Copper 0.5 mM 12 hrs, larvae L3    54 Copper 15 mM 48 hrs, 4-day adult    104 Zinc 5 mM 12 hrs, larvae L3    41 Zinc 4.5 mM 48 hrs, 4-day adult    52 Ethanol 2.5% 3 hrs, larvae L3    80 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    101 Caffeine 25 mg/ml 48 hrs, 4-day adult    80 Paraquat 5 mM 48 hrs, 4-day adult    70 Paraquat 10 mM 48 hrs, 4-day adult    63 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013726 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8705-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | germline clone | non-rescuable maternal effect | recessive pnutrN498 lethal | pupal stage | recessive pnutXP lethal | recessive pnut02502 pnutH2 pnutrN498 pnutrQ348 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 pnutGD1512 viable pnutEY03208 Sterility Allele fertile pnutEY03208 Other Phenotypes Allele cytokinesis defective | recessive pnutXP visible, with Scer\GAL4pnr-MD237 pnutGD1512 wild-type pnut+t10 pnuths.PN Phenotype manifest in Allele actin filament | embryonic stage | germline clone pnutrN498 denticle belt | germline clone pnutrN498 dorsal appendage pnut02502 egg | germline clone pnutrN498 embryonic/first instar larval cuticle | germline clone pnutrN498 embryonic/larval lymph gland pnutXP embryonic head | germline clone pnutrN498 follicle cell pnutXP germarium region 1 | germline clone pnutrN498 imaginal disc pnutXP imaginal ring pnutXP nucleus | embryonic stage | germline clone pnutrN498 photoreceptor cell R7 pnut1 pnutH2 salivary gland common duct primordium pnutXP trichogen cell, with Scer\GAL4pnr-MD237 pnutGD1512 ventral furrow & actin filament | germ-line clone pnutrN498
Classical Alleles ( 15 )
For All Classical Alleles Show
Allele of pnut Class Mutagen Stocks Known lesion pnut02502 P-element activity 2 Yes pnutXP Delta2-3 2 Yes pnutc04668 piggyBac transposase 1 -- pnutDG05807 P-element activity 1 -- pnute02510 piggyBac transposase 1 -- pnutKG00478 P-element activity 1 -- pnut1 ethyl methanesulfonate 0 -- pnutBG01328 P-element activity 0 -- pnutc04715 piggyBac transposase 0 -- pnutEY03208 P-element activity 0 -- pnutH2 0 -- pnutKG00141 P-element activity 0 -- pnutrN498 P-element activity 0 Yes pnutrQ348 P-element activity 0 Yes pnutunspecified 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of pnut Class Mutagen Stocks Known lesion pnutGD1512 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pnut+t10 in vitro construct - genomic fragment 0 Yes pnutdsRNA.337-1266 in vitro construct - RNAi 0 Yes pnuths.PN in vitro construct - regulatory fusion 0 Yes pnutNIG.8705R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)44CE (Spradling et al., 1999, BDGP Project Members, 1994-1999, Neufeld and Rubin, 1994) Df(2R)193A (Neufeld and Rubin, 1994) Df(2R)BSC266 (Christensen et al., 2008.12.28) Df(2R)BSC267 (Christensen et al., 2007.10.29) Df(2R)dpn-2 (Barbash and Cline, 1995) Df(2R)Exel6057 (Ryder, 2004.4.26)
Not disrupted in	Df(2R)CA53 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)cn9 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)H3C1 (BDGP Project Members, 1994-1999) Df(2R)nap16 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hs-pnut} NA UAS construct P{GD1512} NA P{NIG.8705R} NA characterization construct P{pnut} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{GT1}pnutBG01328 NA P{SUPor-P}pnutKG00141 NA P{SUPor-P}pnutKG00478 NA P{wHy}pnutDG05807 NA PBac{PB}pnutc04668 NA PBac{PB}pnutc04715 NA PBac{RB}pnute02510 NA insertion of mobile activating element P{EP}EP2036 NA P{EPgy2}pnutEY03208 NA insertion of enhancer trap P{PZ}pnut02502 No P{PZ}pnutrN498 No P{PZ}pnutrQ348 No insertion of enhancer trap binary system P{GalW}S443 No
Gene Ontology: Function, Process & Cellular Component ( 22 unique terms )
Terms Based on Experimental Evidence ( 11 terms )
Molecular Function
CV term Evidence References
actin binding inferred from direct assay (Sisson et al., 2000) GTPase activity inferred from direct assay (Huijbregts et al., 2009) microtubule binding inferred from direct assay (Sisson et al., 2000) ubiquitin protein ligase binding inferred from physical interaction with park (Bae et al., 2007)
Biological Process
CV term Evidence References
apoptotic process inferred from genetic interaction with W AND inferred from genetic interaction with grim AND inferred from genetic interaction with rpr (Gottfried et al., 2004) cytokinesis inferred from mutant phenotype (Echard et al., 2004) GTP catabolic process inferred from direct assay (Huijbregts et al., 2009) positive regulation of apoptotic process inferred from direct assay (Bae et al., 2007)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008) plasma membrane inferred from direct assay (Huijbregts et al., 2009) septin complex inferred from direct assay (Huijbregts et al., 2009)
Terms Based on Predictions or Assertions ( 12 terms )
Molecular Function
CV term Evidence References
GTP binding inferred from electronic annotation with InterPro:IPR000038 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
cell cycle inferred from electronic annotation with InterPro:IPR000038 (FlyBase Curators et al., 2004-) cellularization traceable author statement (Tepass et al., 2001, Mazumdar and Mazumdar, 2002) compound eye photoreceptor development non-traceable author statement (Swiss-Prot Project Members, 1995.2.1) cytokinesis inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) non-traceable author statement (Swiss-Prot Project Members, 1995.2.1) traceable author statement (Sisson et al., 2000) cytokinesis, actomyosin contractile ring assembly non-traceable author statement (Tepass et al., 2001) vesicle docking involved in exocytosis traceable author statement (Sisson et al., 2000) vesicle targeting traceable author statement (Sisson et al., 2000)
Cellular Component
CV term Evidence References
apical plasma membrane non-traceable author statement (Swiss-Prot Project Members, 1995.2.1) germline ring canal non-traceable author statement (Deng and Lin, 2001) intercellular bridge non-traceable author statement (Swiss-Prot Project Members, 1995.2.1) septin ring inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
pnut - CG3735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) pnut - HIP-R experimental knowledge based (Guruharsha et al., 2011) pnut - Rpn11 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) pnut - Rpn2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) pnut - Rpn8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) pnut - Sep1 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) pnut - Sep2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) pnut - Sep5 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) pnut - robl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions pnut allele Gene References pnut1 grim (Gottfried et al., 2004) rpr (Gottfried et al., 2004) sina (Neufeld and Rubin, 1994) W (Gottfried et al., 2004) pnut02502 Ras85D (Schnorr et al., 2001) S (Gaengel and Mlodzik, 2003) pnutH2 sina (Carthew et al., 1994) pnutXP grim (Gottfried et al., 2004) rpr (Gottfried et al., 2004) S (Gaengel and Mlodzik, 2003) W (Gottfried et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61655 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0013726 DroID - A comprehensive database of gene and protein interactions. • FBgn0013726 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35801
Orthologs
OrthoDB Orthologs (62) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG60K8JR Dipteran inclusive ortholog search EOG651F08 Insect inclusive ortholog search EOG6PZH33 Arthropod inclusive ortholog search EOG6PZGP1 Metazoa inclusive ortholog search EOG68SVMM
Orthologs in Drosophila Species (EOG60K8JR)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\pnut     Drosophila simulans   Dsim\GD10550 Y   Drosophila sechellia   Dsec\GM21017 Y   Drosophila erecta   Dere\GG23345 Y   Drosophila yakuba   Dyak\GE19185 Y   Drosophila ananassae   Dana\GF12347 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21269 Y   Drosophila persimilis   Dper\GL11211 Y   Drosophila willistoni   Dwil\GK15820 Y   Drosophila virilis   Dvir\GJ21698 Y   Drosophila mojavensis   Dmoj\GI18682 Y   Drosophila grimshawi   Dgri\GH21082 Y
Orthologs in non-Drosophila Dipterans (EOG651F08)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001574   Anopheles gambiae Malaria mosquito  Agam\AGAP007596   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000235
Orthologs in non-Dipteran Insects (EOG6PZH33)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB44419   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013271   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16640   Atta cephalotes Leafcutter ant  Acep\ACEP15886   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16419   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16707   Linepithema humile Argentine ant  Lhum\LH23028   Pogonomyrmex barbatus Red harvester ant  Pbar\PB13380   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000885   Bombyx mori Silkmoth  Bmor\BGIBMGA003744   Pediculus humanus Human body louse  Phum\PHUM380240   Tribolium castaneum Red flour beetle  Tcas\TC003373
Orthologs in non-Insect Arthropods (EOG6PZGP1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW018169   Ixodes scapularis Deer tick  Isca\ISCW006074
Orthologs in non-Arthropod Metazoa (EOG68SVMM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\unc-59 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009849gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001250gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003696gn Y Danio rerio Zebrafish  Drer\ENSDARG00000075198 Y Danio rerio Zebrafish  Drer\sept4a Y Danio rerio Zebrafish  Drer\sept2 Y Danio rerio Zebrafish  Drer\zgc:63587 Y Danio rerio Zebrafish  Drer\hm:zeh0351 Y Danio rerio Zebrafish  Drer\sept5b Y Danio rerio Zebrafish  Drer\sept7b Y Xenopus tropicalis Western clawed frog  Xtro\sept7 Y Xenopus tropicalis Western clawed frog  Xtro\sept2-A Y Xenopus tropicalis Western clawed frog  Xtro\sept5 Y Xenopus tropicalis Western clawed frog  Xtro\sept2-B Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000030625 Y Gallus gallus Domestic chicken  Ggal\Sep-05 Y Gallus gallus Domestic chicken  Ggal\SEPT7 Y Gallus gallus Domestic chicken  Ggal\SEPT2 Y Gallus gallus Domestic chicken  Ggal\Sep-02 Y Mus musculus House mouse  Mmus\Sept4 Y Mus musculus House mouse  Mmus\Sept5 Y Mus musculus House mouse  Mmus\Sept7 Y Rattus norvegicus Norway rat  Rnor\Sept7 Y Rattus norvegicus Norway rat  Rnor\Sept2 Y Rattus norvegicus Norway rat  Rnor\Sept4 Y Rattus norvegicus Norway rat  Rnor\Gp1bb Y Rattus norvegicus Norway rat  Rnor\Sept1 Y Homo sapiens Human  Hsap\SEPT5 Y Homo sapiens Human  Hsap\SEPT2 Y Homo sapiens Human  Hsap\ENSG00000108387 Y Homo sapiens Human  Hsap\SEPT1 Y Homo sapiens Human  Hsap\SEPT7 Y
Human Orthologs (5)
Gene OMIM HGNC Hsap\SEPT5     Hsap\SEPT2     Hsap\ENSG00000108387     Hsap\SEPT1     Hsap\SEPT7
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10550 Drosophila sechellia Dsec\GM21017 Drosophila erecta Dere\GG23345 Drosophila yakuba Dyak\GE19185 Drosophila ananassae Dana\GF12347 Drosophila pseudoobscura pseudoobscura Dpse\GA21269 Drosophila persimilis Dper\GL11211 Drosophila willistoni Dwil\GK15820 Drosophila virilis Dvir\GJ21698 Drosophila mojavensis Dmoj\GI18682 Drosophila grimshawi Dgri\GH21082
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	5687 pnutXP/T(2;3)SM6a-TM6B, Tb1 14354 y1; P{SUPor-P}pnutKG00478/SM6a 21071 y1 w67c23; P{wHy}pnutDG05807 11194 cn1 P{PZ}pnut02502/CyO; ry506
Harvard	- PBac{PB}pnutc04668 - PBac{RB}pnute02510
Kyoto	108364 pnutXP/T(2;3)SM6a-TM6B, Tb1 108867 cn1 P{PZ}pnut02502/CyO; ry506
VDRC	v11791 w1118 P{GD1512}v11791
Genomic Clones ( 2 )
	BACR09N11 BACR14B02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 230 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD37170
Other clones	DPiM_LD37170
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH10764 GH12743 GH12846 GH12880 GH16380 GH19735 GH20052 GH20145 GM01444 GM04145 GM17149 GM23113 GM24309 GM28031 LD01040 LD02585 LD04091 LD06550 LD09763 LD12516 LD13214 LD13520 LD14536 LD15817 LD18330 LD20069 LD20881 LD22860 LD26508 LD27478 LD27770 LD31574 LD31692 LD32921 LD33747 LD36556 LD37003 LD39216 LD39706 LD39903 LD40808 LD40926 LD42173 LD42451 LD42694 LD43370 LD45249 LD45319 LD45913 LD46037 LD47437 LP03764 LP06395 LP09353 LP09479 LP11158 LP11166 LP12813 LP19238 RE03630 RE04942 RE05776 RE06884 RE10620 RE12580 RE12801 RE15144 RE18953 RE21778 RE27235 RE27237 RE27252 RE27283 RE28715 RE29430 RE29987 RE30768 RE33210 RE34778 RE35882 RE36181 RE39291 RE52027 RE55467 RE55630 RE55830 RE56757 RE56982 RE58336 RE59987 RE61948 RE62618 RE62648 RE70157 RE70991 RE71287 RE73328 RH04593 RH05641 RH06848 RH21684 RH30185 RH38303 RH53796 RH57839 RH69557 RH69795 SD02244 SD02483 SD04126 SD05218 SD08208 SD14511 SD18447 SD18514 SD22980 SD27787
Other clones	13813 17069 60973 66152 97654 101086 124562 144260 144360 147124 147126 178887 214249.32 528214 558125 569913 AI124305 CK01.31B.A4 CK01.65C.H1 CK01.75B.G10 CK01.118A.G12 Dmel_dig_005516_0627_3376 Dmel_dig_009569_0615_2857 EK053329 EK058959 EK090745 EK100308 EK115251 EK128944 EK185710 EK220034 EK223626 EK246916 EK250425 EK250745 EK250754 EK284916 EN09049 EN13035 EN15855 ESG014d.E21_D12 IP12729 IP13860 LD01320 LD02412 LD03402 LD06011 LD06522 LD08141 LD11280 LD12754 LD14079 LD15486 LD16967 LD17907 LD18618 LD19506 LD32481 LD34019 RE01356 RE01556 RE01578 RE05531 RE12456 RE35911 RE59111 RH33645 RH61730 RP004007260 RP004030567 RP004049678 RP004079642 RP004090381 RP004091872 RP004115605 RP004133079 RP004133747 RP004147343 RP004152969 RP004165016 RP004193719 RP004197120 RP004211453 RP004213776 RP004221125 RP004225027 RP004237602 RP004238639 RP004243089 RP004244981 RP004245039 RP004247736 RP004270015 RP004386629 RP004390803 RP004400439 RP004402751 RP004411173 RP004417896 RP004420902 RP004422140 RP004423338 RP004426448 RP004486314 RP004492485 RP004510127 RP004515970 RP004519694 RP004538553 RP004554059 RP004555753
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013726 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35801
Antibody Information
	monoclonal (Neufeld and Rubin, 1994) polyclonal (Huijbregts et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	dsRNA made from templates generated with primers directed against pnut that is transfected into S2 treated with Listeria monocytogenes reveals pnut to be involved in Listeria monocytogenes intracellular growth, with less intracellular growth observed in pnut-treated cells. pnut also seems to be involved in L.monocytogenes entry. (Cheng et al., 2005) dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells. (Echard et al., 2004) During prophase, in nuf-derived embryos, scra and pnut localise to regions of the furrows lacking actin. (Rothwell et al., 1998) A complex of septin polypeptides is isolated that bind and hydrolyse GTP and form filaments. Their high degree of conservation, ubiquitous expression and proven role in cytokinesis suggests septins are certain to be important players in regulating cell architecture and function. Septins alone can form regular filamentous polymers, since filament formation is likely to be central to their localisation and function. (Field et al., 1996) Immunolocalisation and biochemical data suggest that Sep1 and pnut probably function as parts of a complex. (Fares et al., 1995) pnut gene product may be required for normal sina activity. Mutations at pnut affect photoreceptor development and cell proliferation or survival as FLP/FRT mediated clones were smaller than the wild type twin spots. (Carthew et al., 1994) The pnut locus was identified in a screen for mutations that disrupt eye development, and shows genetic interactions with sina. The pnut gene product is required for cytokinesis in imaginal tissues. (Neufeld and Rubin, 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057716 NM_165597 RefSeq (Proteins) • NP_477064 NP_724659 DDBJ / EMBL / GenBank • AAA19603 AAF59112 AAL13892 AAM68857 AC008370 AI061672 AI124305 AI456980 AQ073270 AW943371 AY058663 BH840394 BH840409 BH840410 CL705913 CZ469413 CZ469459 CZ480290 U08103 Entrez Gene - A searchable database of RefSeq genes. • 35801 UniProtKB/Swiss-Prot • P40797
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Cell division protein GTP binding (IPR000038) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61655 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0013726 DroID - A comprehensive database of gene and protein interactions. • FBgn0013726 DRSC - Results from RNAi screens. • FBgn0013726 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013726 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8705-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013726 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35801 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/peanut1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35801 modMine - Data generated by the modENCODE project. • FBgn0013726
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	anon-EST:Liang-2.18   CG8705 (Arbeitman et al., 2004, Goldstein and Gunawardena, 2000, Hadar et al., 2012, Perkins et al., 2009, Hughes et al., 2008) clone 2.18 (Liang and Biggin, 1998) E(sina)8 (Carthew et al., 1994) E(sina)44C   l(2)02502   Pnut (Shih et al., 1998, Adam et al., 1998, Adam et al., 1997, Fares et al., 1995, Padash Barmchi et al., 2005, Ji et al., 2005, Bae et al., 2007, Hickson and O'Farrell, 2008, Hughes et al., 2008, Huijbregts et al., 2009, Kramerova and Kramerov, 1999, Goldbach et al., 2010, Saulière et al., 2010, Fisher et al., 2008) pnut (Altenhein et al., 2006, Sandmann et al., 2007, Hadar et al., 2012, Christensen et al., 2007.10.29, Bakal et al., 2007, Kaltenbach et al., 2007, Christensen et al., 2008.12.28, Choksi et al., 2006, Font-Burgada et al., 2008, D'Avino et al., 2008, Liebl and Featherstone, 2005, Burgio et al., 2008, Huijbregts et al., 2009, Lowery et al., 2010, Pan et al., 2007, Japanese National Institute of Genetics, 2012.5.21) PNUT (Muñoz-Soriano and Paricio, 2007, Monzo et al., 2010) unnamed (Anonymous, 1996)
Name Synonym	Enhancer of seven in absentia 8 (Carthew et al., 1994) Peanut (Sisson et al., 2000, Deshpande et al., 2006, Gilliland et al., 2007, Prokopenko and Chia, 2007, Prokopenko and Chia, 2008, Muñoz-Soriano and Paricio, 2007, Silverman-Gavrila et al., 2008, Sokac and Wieschaus, 2008, D'Avino et al., 2008, D'Avino et al., 2006, Mavrakis et al., 2009, Monzo et al., 2010) peanut (Rothwell et al., 1997, Oegema et al., 1997, Field et al., 1997, Kaltenbach et al., 2007, Castoreno et al., 2010, Mudiganti et al., 2010, Gatt and Glover, 2006, Shalaby et al., 2009, Goldbach et al., 2010, Lowery et al., 2010) septin (Sisson et al., 2000, Guild et al., 1999) Septin (Sokac and Wieschaus, 2008) Septin 3 (D'Avino et al., 2008) septinPnut (Hickson and O'Farrell, 2008)
Secondary FlyBase IDs
	FBgn0010304 FBgn0010515
References ( 150 )
Generate a list of	All references relating to this gene ( 150 )
List References by type	Research paper  ( 80 ) Supplementary material  ( 7 ) Review  ( 16 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 26 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 1 ) Conference report  ( 1 )
Recent research papers ( 4 )
	Housden et al., 2013, PLoS Genet. 9(1): e1003162 Transcriptional Dynamics Elicited by a Short Pulse of Notch Activation Involves Feed-Forward Regulation by E(spl)/Hes Genes. [FBrf0220474] Bras et al., 2012, J. Cell Sci. 125(20): 4886--4901 Genetic identification of intracellular trafficking regulators involved in Notch-dependent binary cell fate acquisition following asymmetric cell division. [FBrf0220239] Hadar et al., 2012, Gene 494(1): 65--72 A screen identifying genes responsive to Dpp and Wg signaling in the Drosophila developing wing. [FBrf0217293] Kechad et al., 2012, Curr. Biol. 22(3): 197--203 Anillin Acts as a Bifunctional Linker Coordinating Midbody Ring Biogenesis during Cytokinesis. [FBrf0217440] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...