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Gene Dmel\shot

General Information
Symbol	Dmel\shot	Species	D. melanogaster
Name	short stop	Annotation symbol	CG18076
Feature type	protein_coding_gene	FlyBase ID	FBgn0013733
Gene Model Status	Current	Stock availability	47 publicly available
Also Known As	kak, grv, l(2)k04204
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	50C6-50C9	Sequence location	2R:9,751,742..9,829,615 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene short stop is referred to in FlyBase by the symbol Dmel\shot (CG18076, FBgn0013733). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: negative regulation of microtubule depolymerization; dendrite morphogenesis; oocyte fate determination; regulation of axon extension; microtubule cytoskeleton organization; mushroom body development; open tracheal system development; branch fusion, open tracheal system; lumen formation, open tracheal system; axon midline choice point recognition. 80 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; late extended germ band embryo; multi-cell-component structure; primordium; ectoderm derivative; embryonic/larval neuron; integumentary specialisation; portion of tissue; presumptive embryonic/larval nervous system. It has 22 annotated transcripts and 22 annotated polypeptides. Protein features are: Actinin-type, actin-binding, conserved site; Calcium-binding EF-hand; Calponin homology domain; EF-HAND 2; EF-Hand 1, calcium-binding site; EF-hand-like domain; Growth-arrest-specific protein 2 domain; Plectin repeat; Spectrin repeat; Spectrin/alpha-actinin; Src homology-3 domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval midgut, adult Malpighian tubules, larval fat body, adult ovary, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1633745_a_at completely aligns to an exonic region common to each of the 15 FlyBase-annotated transcript isoforms of shot. Gene sequence location is 2R:9751742..9829615.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References Lowery et al., 2010, Genetics 185(4): 1311--1325 Mui et al., 2011, PLoS ONE 6(10): e25965 Sequence features BKN21543 HFA05460 HFA07682 HFA05459
FB2011_10	Alleles shot[MI03583] References Bellen, 2011.10.10, MISet953.xml Knowles-Barley, 2011.8.24, BrainTrap expression curation. Insertions Mi{MIC}shot[MI03583]
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 50C6-50C9   Limits computationally determined from genome sequence between P{lacW}l(2)03105k16702&P{lacW}fl(2)dk16105 and P{lacW}shotk03010
Experimentally Determined Cytological Location
Cytogenetic map Notes References 50C6-50C10     (Lee, 1999.10.29) 50C3-50C6     (Strumpf, 1996.11.7) 50C11-50C12   (determined by in situ hybridisation) 50C9--10 (determined by in situ hybridisation)   (Spradling et al., 1999) 50C3-50C6   (determined by in situ hybridisation)   (Strumpf and Volk, 1998) 50C3-50C4   (determined by in situ hybridisation)   (Volk, 1995.4.17) 50B1-50B2   50C11--12 50C11--15 50C14--15 50C9--10 50C9--12   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (van Vactor et al., 1993, Kolodziej et al., 1995, Prout et al., 1997, Prokop et al., 1998)
Left of (cM)	px (Prokop et al., 1998)
Right of (cM)	pr (Prokop et al., 1998)
Notes	Located on 2R. (Prout et al., 1997) Maps 1.5 units on either side of c. (Prokop et al., 1998)
Gene Model & Products
Please see the GBrowse view of Dmel\shot for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Annotated transcripts may not represent all possible combinations of alternative exons and/or alternative promoters.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) shot-RA FBtr0087619  NM_079009   18014   5501 shot-RAA FBtr0304850     17845   5408 shot-RAB FBtr0304851     13661   4100 shot-RAC FBtr0304852     17813   5462 shot-RAD FBtr0304853     17148   5434 shot-RB FBtr0087616  NM_166016   17791   5390 shot-RC FBtr0087620  NM_166018   16907   5160 shot-RE FBtr0087618  NM_166017   17114   5201 shot-RG FBtr0087617  NM_166015   17776   5385 shot-RH FBtr0087621  NM_166019   27842   8805 shot-RI FBtr0273222  NM_001144185   17746   5375 shot-RJ FBtr0273223  NM_001144186   17725   5368 shot-RK FBtr0273224  NM_001144187   17731   5370 shot-RL FBtr0273225  NM_001144188   17969   5486 shot-RM FBtr0273226  NM_001144189   17948   5479 shot-RN FBtr0301591  NM_001169677   17785   5388 shot-RO FBtr0301592  NM_001169678   18008   5499 shot-RP FBtr0301593  NM_001169679   17801   5458 shot-RQ FBtr0301964  NM_001169676   16892   5155 shot-RX FBtr0304847     17729   5434 shot-RY FBtr0304848     18016   5463 shot-RZ FBtr0304849     17803   5394
Additional Transcript Data & Comments
Reported size (kB)	17.6, 15.4 (northern blot) (Strumpf and Volk, 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein shot-PA   FBpp0086745   627.7   5501   6.54     NP_523733   AAF58319 shot-PAA   FBpp0293390   617.5   5408   6.38       shot-PAB   FBpp0293391   465.0   4100   6.25       shot-PAC   FBpp0293392   622.6   5462   6.45       shot-PAD   FBpp0293393   619.3   5434   6.39       shot-PB   FBpp0086742   615.3   5390   6.38     NP_725336   AAG22268 shot-PC   FBpp0086746   589.0   5160   6.41     NP_725338   AAM68562 shot-PE   FBpp0086744   594.6   5201   6.52     NP_725337   AAM68561 shot-PG   FBpp0086743   615.2   5385   6.36     NP_725335   AAF58317 shot-PH   FBpp0086747   989.5   8805   5.41     NP_725339   AAF58320 shot-PI   FBpp0271730   613.7   5375   6.34     NP_001137657   ACL83111 shot-PJ   FBpp0271731   613.1   5368   6.35     NP_001137658   ACL83112 shot-PK   FBpp0271732   613.6   5370   6.32     NP_001137659   ACL83113 shot-PL   FBpp0271733   626.1   5486   6.51     NP_001137660   ACL83114 shot-PM   FBpp0271734   625.5   5479   6.52     NP_001137661   ACL83115 shot-PN   FBpp0290806   615.4   5388   6.37     NP_001163148   ACZ94420 shot-PO   FBpp0290807   627.7   5499   6.53     NP_001163149   ACZ94421 shot-PP   FBpp0290808   622.1   5458   6.42     NP_001163150   ACZ94422 shot-PQ   FBpp0291176   588.9   5155   6.38     NP_001163147   ACZ94419 shot-PX   FBpp0293387   619.3   5434   6.39       shot-PY   FBpp0293388   622.2   5463   6.40       shot-PZ   FBpp0293389   616.2   5394   6.40
Additional Polypeptide Data & Comments
Reported size (kDa)	5201 (aa) (Lee et al., 2000)
Comments	shot protein shows regions of similarity to three distinct vertebrate cytoskeletal-related protein families. The amino-terminal portion resembles the amino-terminal domain of plakin family members. The central portion has 22 repeats of 105-113 amino acids that are similar to the spectrin-like repeats found in dystrophin, α-actinin, and spectrin. The carboxy terminal domain shows similarity to the Gar2/GAR22 family of cytoskeletal-related proteins. (Lee et al., 2000) The shot protein shows similarily to three distinct classes of proteins. The amino-terminal portion resembles the plakin class of cytoskeletal cross-linker proteins. The central portion of the protein consists of 22 109aa dystrophin-like repeats. The carboxy-terminal part of the protein has similarity to Gas2, an actin-associated protein expressed in growth-arrested cultured cells. (Gregory and Brown, 1998)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Spectrin/alpha-actinin (IPR018159) EF-HAND 2 (IPR018249) EF-Hand 1, calcium-binding site (IPR018247) Plectin repeat (IPR001101) Actinin-type, actin-binding, conserved site (IPR001589) Calponin homology domain (IPR001715) Spectrin repeat (IPR002017) Calcium-binding EF-hand (IPR002048) Growth-arrest-specific protein 2 domain (IPR003108) EF-hand-like domain (IPR011992) Src homology-3 domain (IPR001452)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA697956   AA820149   AE013599 AAF58317   AE013599 AAF58319   AE013599 AAF58320   AE013599 AAG22268   AE013599 AAM68561   AE013599 AAM68562   AE013599 ACL83111   AE013599 ACL83112   AE013599 ACL83113   AE013599 ACL83114   AE013599 ACL83115   AE013599 ACZ94419   AE013599 ACZ94420   AE013599 ACZ94421   AE013599 ACZ94422   AF200422 AAF24340   AF200423 AAF24341   AF200424 AAF24342   AF200425 AAF24343   AI107881   AI135908   AI135930   AI258450   AI296409   AI404028   AI404052   AI517171   AI519702   AI520158   AJ011924 CAA09869   AJ011925 CAA09870   AQ025736   AQ025944   AQ034139   AW943466   AY122181 AAM52693   BG631943   BG633248   BG635279   BG638972   BH615092   BI170806   BI230182   BI564872   BI565814   BI566006   BI568033   BI569870   BI571025   BI572498   BI591096   BI591131   BI607529   BI610151   BI611641   BI613041   BI613867   BI621401   BI623332   BI624566   BI624920   BI625936   BI635876   BI637836   BP540384   BT025072   BT056290   BT056292   BZ665554   CA807537   CO177592   CO187455   CO187989   CO265763   CO276965   CO280641   CO292757   CO293935   CO294129   CO305326   CO308439   CO313797   CO314669   CO315328   CO318840   CO330363   CO337245   CO337667   CO337840   CO340012   CO344047   CO344619   CZ466770   CZ466819   CZ468531   CZ469968   CZ471298   CZ471781   CZ472126   CZ472770   CZ473003   CZ473275   CZ474300   CZ474301   CZ474358   CZ474940   CZ476202   CZ477008   CZ489617   CZ490504   DN153828   DN153988   EC059879   EC061404   EC065482   EC073071   EC079016   EC083121   EC084809   EC086502   EC088022   EC195221   EC195657   EC206544   EC214527   EC215043   EC215843   EC224349   EC226704   EC227059   EC227848   EC240819   EC247041   EC252428   EC252532   EC257488   EC259226   EC266974   EC267051   EC267946   EL870855   ES690207   ES690926   ES696445   GH951160   GH960482   GH962412   GH962456   GH984447   Y09430 CAA70581
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O77291 O96936 Q7KAJ2 Q7KJN8 Q7KJN9 Q8MR19 Q9U479 A1Z9J0 A1Z9I8 A1Z9I9 A1Z9J2 A1Z9J1 A1Z9J3 B7YZG6 B7YZG4 B7YZG2 B7YZG3 B7YZG5 E1JH64 E1JH62 E1JH63 E1JH65
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   epidermis   (Lee et al., 2000) muscle attachment site   (Lee et al., 2000) embryonic stage 11 -- 17   epidermis   (Strumpf and Volk, 1998) organism | segmentally repeated   (Strumpf and Volk, 1998) embryonic stage 13 -- 17   presumptive embryonic/larval peripheral nervous system   (Lee et al., 2000) presumptive embryonic/larval central nervous system   (Lee et al., 2000) embryonic stage 16   muscle attachment site   (Strumpf and Volk, 1998)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   sensory neuron   (Lee et al., 2000) intersegmental nerve   (Lee et al., 2000) chordotonal organ   (Lee et al., 2000) embryonic stage 12 -- 17   neuron   • axon (Lee et al., 2000) ectoderm of keyhole structure   • cell cortex (Fuss et al., 2004) embryonic stage 14 -- 17   muscle attachment site   (Strumpf and Volk, 1998) embryonic stage 16   muscle attachment site   (Gregory and Brown, 1998) scolopidium   (Gregory and Brown, 1998) embryonic/larval pharynx   (Gregory and Brown, 1998) embryonic/larval proventriculus   (Gregory and Brown, 1998) tendon cell   (Strumpf and Volk, 1998) chordotonal organ   (Strumpf and Volk, 1998) epidermis   (Strumpf and Volk, 1998) third instar larval stage   tendon cell   • microtubule cytoskeleton (Subramanian et al., 2003) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
	shot protein is expressed in specific epidermal cells that attach to muscles, linking the muscles to the exoskeleton. It is strongly expressed in the same cells that express high levels of PS integrins. Strong expression is also observed in the proventriculus and the pharynx. Weak expression is observed in the PNS in the scolopale of the chordotonal organs. (Gregory and Brown, 1998) shot protein is apparent from stage 14 of embryogenesis. In the last stages of muscle development, shot protein is found at high levels in muscle-bound tendon cells where it is concentrated at the surface, presumably along the membrane. Lower levels are observed in epidermal cells and chordotonal organs. (Strumpf and Volk, 1998) The shot protein colocalizes with microtubules in mature tendon cells of third instar larvae. There is a focus of staining overlapping a compact microtubule array near the muscle-tendon junction. This unique subcellular structure extends to the cuticle attachment site. (Subramanian et al., 2003) shot protein localizes to the apical side of the ectodermal domain of the keyhole structure of the developing embryonic proventriculus. (Fuss et al., 2004) shot protein is detected in developing axons starting in embryonic stage 12. It can be detected in the intersegmental nerve and in the cortical regions of the neuronal cell bodies of chordotonal and dorsal cluster sensory neurons. The antibody used for this analysis does not recognize the short isoforms of the protein. (Lee et al., 2000)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0013733    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0013733 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 18-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0013733 expression level Developmental Stage   Expression Level embryo 00-02hr    408 embryo 02-04hr    501 embryo 04-06hr    1046 embryo 06-08hr    1189 embryo 08-10hr    1138 embryo 10-12hr    598 embryo 12-14hr    1385 embryo 14-16hr    1980 embryo 16-18hr    2974 embryo 18-20hr    5643 embryo 20-22hr    5128 embryo 22-24hr    4830 larva L1    2039 larva L2    1399 larva L3 12hr old    2620 larva L3 puffstage 1-2    777 larva L3 puffstage 3-6    1967 larva L3 puffstage 7-9    1644 white prepupae new    1210 white prepupae 12hr    1763 white prepupae 24hr    1522 pupae 2d postWPP    1374 pupae 3d postWPP    1999 pupae 4d postWPP    3858 adult male 01day    3339 adult male 05day    3031 adult male 30day    2100 adult female 01day    1765 adult female 05day    941 adult female 30day    960 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (408) embryo 02-04hr  (501) embryo 04-06hr  (1046) embryo 06-08hr  (1189) embryo 08-10hr  (1138) embryo 10-12hr  (598) embryo 12-14hr  (1385) embryo 14-16hr  (1980) embryo 16-18hr  (2974) embryo 18-20hr  (5643) embryo 20-22hr  (5128) embryo 22-24hr  (4830) larva L1  (2039) larva L2  (1399) larva L3 12hr old  (2620) larva L3 puffstage 1-2  (777) larva L3 puffstage 3-6  (1967) larva L3 puffstage 7-9  (1644) white prepupae new  (1210) white prepupae 12hr  (1763) white prepupae 24hr  (1522) pupae 2d postWPP  (1374) pupae 3d postWPP  (1999) pupae 4d postWPP  (3858) adult male 01day  (3339) adult male 05day  (3031) adult male 30day  (2100) adult female 01day  (1765) adult female 05day  (941) adult female 30day  (960) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    408 embryo 02-04hr    501 embryo 04-06hr    1046 embryo 06-08hr    1189 embryo 08-10hr    1138 embryo 10-12hr    598 embryo 12-14hr    1385 embryo 14-16hr  (1980) embryo 16-18hr  (2974) embryo 18-20hr  (5643) embryo 20-22hr  (5128) embryo 22-24hr  (4830) larva L1  (2039) larva L2    1399 larva L3 12hr old  (2620) larva L3 puffstage 1-2    777 larva L3 puffstage 3-6  (1967) larva L3 puffstage 7-9  (1644) white prepupae new    1210 white prepupae 12hr  (1763) white prepupae 24hr  (1522) pupae 2d postWPP    1374 pupae 3d postWPP  (1999) pupae 4d postWPP  (3858) adult male 01day  (3339) adult male 05day  (3031) adult male 30day  (2100) adult female 01day  (1765) adult female 05day    941 adult female 30day    960 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    408 embryo 02-04hr    501 embryo 04-06hr    1046 embryo 06-08hr    1189 embryo 08-10hr    1138 embryo 10-12hr    598 embryo 12-14hr    1385 embryo 14-16hr    1980 embryo 16-18hr    2974 embryo 18-20hr    5643 embryo 20-22hr    5128 embryo 22-24hr    4830 larva L1    2039 larva L2    1399 larva L3 12hr old    2620 larva L3 puffstage 1-2    777 larva L3 puffstage 3-6    1967 larva L3 puffstage 7-9    1644 white prepupae new    1210 white prepupae 12hr    1763 white prepupae 24hr    1522 pupae 2d postWPP    1374 pupae 3d postWPP    1999 pupae 4d postWPP    3858 adult male 01day    3339 adult male 05day    3031 adult male 30day    2100 adult female 01day    1765 adult female 05day    941 adult female 30day    960 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    408 embryo 02-04hr    501 embryo 04-06hr    1046 embryo 06-08hr    1189 embryo 08-10hr    1138 embryo 10-12hr    598 embryo 12-14hr    1385 embryo 14-16hr    1980 embryo 16-18hr    2974 embryo 18-20hr    5643 embryo 20-22hr    5128 embryo 22-24hr    4830 larva L1    2039 larva L2    1399 larva L3 12hr old    2620 larva L3 puffstage 1-2    777 larva L3 puffstage 3-6    1967 larva L3 puffstage 7-9    1644 white prepupae new    1210 white prepupae 12hr    1763 white prepupae 24hr    1522 pupae 2d postWPP    1374 pupae 3d postWPP    1999 pupae 4d postWPP    3858 adult male 01day    3339 adult male 05day    3031 adult male 30day    2100 adult female 01day    1765 adult female 05day    941 adult female 30day    960 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0013733 expression level Developmental Stage   Expression Level embryo 00-02hr    408 embryo 02-04hr    501 embryo 04-06hr    1046 embryo 06-08hr    1189 embryo 08-10hr    1138 embryo 10-12hr    598 embryo 12-14hr    1385 embryo 14-16hr    1980 embryo 16-18hr    2974 embryo 18-20hr    5643 embryo 20-22hr    5128 embryo 22-24hr    4830 larva L1    2039 larva L2    1399 larva L3 12hr old    2620 larva L3 puffstage 1-2    777 larva L3 puffstage 3-6    1967 larva L3 puffstage 7-9    1644 white prepupae new    1210 white prepupae 12hr    1763 white prepupae 24hr    1522 pupae 2d postWPP    1374 pupae 3d postWPP    1999 pupae 4d postWPP    3858 adult male 01day    3339 adult male 05day    3031 adult male 30day    2100 adult female 01day    1765 adult female 05day    941 adult female 30day    960 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (408) embryo 02-04hr  (501) embryo 04-06hr  (1046) embryo 06-08hr  (1189) embryo 08-10hr  (1138) embryo 10-12hr  (598) embryo 12-14hr  (1385) embryo 14-16hr  (1980) embryo 16-18hr  (2974) embryo 18-20hr  (5643) embryo 20-22hr  (5128) embryo 22-24hr  (4830) larva L1  (2039) larva L2  (1399) larva L3 12hr old  (2620) larva L3 puffstage 1-2  (777) larva L3 puffstage 3-6  (1967) larva L3 puffstage 7-9  (1644) white prepupae new  (1210) white prepupae 12hr  (1763) white prepupae 24hr  (1522) pupae 2d postWPP  (1374) pupae 3d postWPP  (1999) pupae 4d postWPP  (3858) adult male 01day  (3339) adult male 05day  (3031) adult male 30day  (2100) adult female 01day  (1765) adult female 05day  (941) adult female 30day  (960) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    408 embryo 02-04hr    501 embryo 04-06hr    1046 embryo 06-08hr    1189 embryo 08-10hr    1138 embryo 10-12hr    598 embryo 12-14hr    1385 embryo 14-16hr  1980 embryo 16-18hr  (2974) embryo 18-20hr  (5643) embryo 20-22hr  (5128) embryo 22-24hr  (4830) larva L1  2039 larva L2    1399 larva L3 12hr old  (2620) larva L3 puffstage 1-2    777 larva L3 puffstage 3-6  1967 larva L3 puffstage 7-9  1644 white prepupae new    1210 white prepupae 12hr  1763 white prepupae 24hr  1522 pupae 2d postWPP    1374 pupae 3d postWPP  1999 pupae 4d postWPP  (3858) adult male 01day  (3339) adult male 05day  (3031) adult male 30day  2100 adult female 01day  1765 adult female 05day    941 adult female 30day    960 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    408 embryo 02-04hr    501 embryo 04-06hr    1046 embryo 06-08hr    1189 embryo 08-10hr    1138 embryo 10-12hr    598 embryo 12-14hr    1385 embryo 14-16hr    1980 embryo 16-18hr    2974 embryo 18-20hr    5643 embryo 20-22hr    5128 embryo 22-24hr    4830 larva L1    2039 larva L2    1399 larva L3 12hr old    2620 larva L3 puffstage 1-2    777 larva L3 puffstage 3-6    1967 larva L3 puffstage 7-9    1644 white prepupae new    1210 white prepupae 12hr    1763 white prepupae 24hr    1522 pupae 2d postWPP    1374 pupae 3d postWPP    1999 pupae 4d postWPP    3858 adult male 01day    3339 adult male 05day    3031 adult male 30day    2100 adult female 01day    1765 adult female 05day    941 adult female 30day    960 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    408 embryo 02-04hr    501 embryo 04-06hr    1046 embryo 06-08hr    1189 embryo 08-10hr    1138 embryo 10-12hr    598 embryo 12-14hr    1385 embryo 14-16hr    1980 embryo 16-18hr    2974 embryo 18-20hr    5643 embryo 20-22hr    5128 embryo 22-24hr    4830 larva L1    2039 larva L2    1399 larva L3 12hr old    2620 larva L3 puffstage 1-2    777 larva L3 puffstage 3-6    1967 larva L3 puffstage 7-9    1644 white prepupae new    1210 white prepupae 12hr    1763 white prepupae 24hr    1522 pupae 2d postWPP    1374 pupae 3d postWPP    1999 pupae 4d postWPP    3858 adult male 01day    3339 adult male 05day    3031 adult male 30day    2100 adult female 01day    1765 adult female 05day    941 adult female 30day    960 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0013733    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0013733 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval midgut, adult Malpighian tubules, larval fat body, adult ovary, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0013733 expression level Tissue   Expression Level Larval Central Nervous System    704.325 Larval Midgut    479.4 Larval Hindgut    1152.9 Larval Malpighian Tubules    540.8 Larval Fat Body    364.3 Larval Salivary Gland    621.9 Larval Trachea    2337.2 Larval Carcass    1312.825 Adult Head    574.1 Adult Eye    956.9 Adult Brain    715.5 Adult Thoracic-Abdominal Ganglion    709.1 Adult Crop    1289.7 Adult Midgut    508.2 Adult Hindgut    886.5 Adult Malpighian Tubules    234.8 Adult Fat Body    549.8 Adult Salivary Gland    518.2 Adult Heart    702.5 Adult VirginFemale Spermatheca    584.6 Adult InseminatedFemale Spermatheca    930.6 Adult Ovary    181.2 Adult Testis    83.6 Adult Male Accessory Gland    417.5 Adult Carcass    976.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (704.325) Larval Midgut    479.4 Larval Hindgut  (1152.9) Larval Malpighian Tubules  (540.8) Larval Fat Body    364.3 Larval Salivary Gland  (621.9) Larval Trachea  (2337.2) Larval Carcass  (1312.825) Adult Head  (574.1) Adult Eye  (956.9) Adult Brain  (715.5) Adult Thoracic-Abdominal Ganglion  (709.1) Adult Crop  (1289.7) Adult Midgut  508.2 Adult Hindgut  (886.5) Adult Malpighian Tubules    234.8 Adult Fat Body  (549.8) Adult Salivary Gland  518.2 Adult Heart  (702.5) Adult VirginFemale Spermatheca  (584.6) Adult InseminatedFemale Spermatheca  (930.6) Adult Ovary    181.2 Adult Testis    83.6 Adult Male Accessory Gland    417.5 Adult Carcass  (976.7) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    704.325 Larval Midgut    479.4 Larval Hindgut  (1152.9) Larval Malpighian Tubules    540.8 Larval Fat Body    364.3 Larval Salivary Gland    621.9 Larval Trachea  (2337.2) Larval Carcass  (1312.825) Adult Head    574.1 Adult Eye    956.9 Adult Brain    715.5 Adult Thoracic-Abdominal Ganglion    709.1 Adult Crop  (1289.7) Adult Midgut    508.2 Adult Hindgut    886.5 Adult Malpighian Tubules    234.8 Adult Fat Body    549.8 Adult Salivary Gland    518.2 Adult Heart    702.5 Adult VirginFemale Spermatheca    584.6 Adult InseminatedFemale Spermatheca    930.6 Adult Ovary    181.2 Adult Testis    83.6 Adult Male Accessory Gland    417.5 Adult Carcass    976.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    704.325 Larval Midgut    479.4 Larval Hindgut    1152.9 Larval Malpighian Tubules    540.8 Larval Fat Body    364.3 Larval Salivary Gland    621.9 Larval Trachea    2337.2 Larval Carcass    1312.825 Adult Head    574.1 Adult Eye    956.9 Adult Brain    715.5 Adult Thoracic-Abdominal Ganglion    709.1 Adult Crop    1289.7 Adult Midgut    508.2 Adult Hindgut    886.5 Adult Malpighian Tubules    234.8 Adult Fat Body    549.8 Adult Salivary Gland    518.2 Adult Heart    702.5 Adult VirginFemale Spermatheca    584.6 Adult InseminatedFemale Spermatheca    930.6 Adult Ovary    181.2 Adult Testis    83.6 Adult Male Accessory Gland    417.5 Adult Carcass    976.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0013733 expression level Tissue   Expression Level Larval Central Nervous System    704.325 Larval Midgut    479.4 Larval Hindgut    1152.9 Larval Malpighian Tubules    540.8 Larval Fat Body    364.3 Larval Salivary Gland    621.9 Larval Trachea    2337.2 Larval Carcass    1312.825 Adult Head    574.1 Adult Eye    956.9 Adult Brain    715.5 Adult Thoracic-Abdominal Ganglion    709.1 Adult Crop    1289.7 Adult Midgut    508.2 Adult Hindgut    886.5 Adult Malpighian Tubules    234.8 Adult Fat Body    549.8 Adult Salivary Gland    518.2 Adult Heart    702.5 Adult VirginFemale Spermatheca    584.6 Adult InseminatedFemale Spermatheca    930.6 Adult Ovary    181.2 Adult Testis    83.6 Adult Male Accessory Gland    417.5 Adult Carcass    976.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  704.325 Larval Midgut    479.4 Larval Hindgut  (1152.9) Larval Malpighian Tubules  540.8 Larval Fat Body    364.3 Larval Salivary Gland  621.9 Larval Trachea  (2337.2) Larval Carcass  (1312.825) Adult Head  574.1 Adult Eye  (956.9) Adult Brain  715.5 Adult Thoracic-Abdominal Ganglion  709.1 Adult Crop  (1289.7) Adult Midgut  508.2 Adult Hindgut  (886.5) Adult Malpighian Tubules    234.8 Adult Fat Body  549.8 Adult Salivary Gland  518.2 Adult Heart  702.5 Adult VirginFemale Spermatheca  584.6 Adult InseminatedFemale Spermatheca  (930.6) Adult Ovary    181.2 Adult Testis    83.6 Adult Male Accessory Gland    417.5 Adult Carcass  (976.7) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    704.325 Larval Midgut    479.4 Larval Hindgut  1152.9 Larval Malpighian Tubules    540.8 Larval Fat Body    364.3 Larval Salivary Gland    621.9 Larval Trachea  (2337.2) Larval Carcass  1312.825 Adult Head    574.1 Adult Eye    956.9 Adult Brain    715.5 Adult Thoracic-Abdominal Ganglion    709.1 Adult Crop  1289.7 Adult Midgut    508.2 Adult Hindgut    886.5 Adult Malpighian Tubules    234.8 Adult Fat Body    549.8 Adult Salivary Gland    518.2 Adult Heart    702.5 Adult VirginFemale Spermatheca    584.6 Adult InseminatedFemale Spermatheca    930.6 Adult Ovary    181.2 Adult Testis    83.6 Adult Male Accessory Gland    417.5 Adult Carcass    976.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    704.325 Larval Midgut    479.4 Larval Hindgut    1152.9 Larval Malpighian Tubules    540.8 Larval Fat Body    364.3 Larval Salivary Gland    621.9 Larval Trachea    2337.2 Larval Carcass    1312.825 Adult Head    574.1 Adult Eye    956.9 Adult Brain    715.5 Adult Thoracic-Abdominal Ganglion    709.1 Adult Crop    1289.7 Adult Midgut    508.2 Adult Hindgut    886.5 Adult Malpighian Tubules    234.8 Adult Fat Body    549.8 Adult Salivary Gland    518.2 Adult Heart    702.5 Adult VirginFemale Spermatheca    584.6 Adult InseminatedFemale Spermatheca    930.6 Adult Ovary    181.2 Adult Testis    83.6 Adult Male Accessory Gland    417.5 Adult Carcass    976.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    574.1   NA  Eye    956.9   NA  Brain    715.5   704.325  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    709.1   NA  Crop    1289.7   479.4  Midgut    508.2   1152.9  Hindgut    886.5   540.8  Malpighian Tubules    234.8   364.3  Fat Body    549.8   621.9  Salivary Gland    518.2   NA  Heart    702.5   2337.2  Trachea    NA   NA  VirginFemale Spermatheca    584.6   NA  InseminatedFemale Spermatheca    930.6   NA  Ovary    181.2   NA  Testis    83.6   NA  Male Accessory Gland    417.5   1312.825  Carcass    976.7 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_24
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013733 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18076-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | recessive shot91k shotel3 shotHG25 shotSF20 shotV104 shotV167 lethal | germline clone shot3 lethal | larval stage, with Scer\GAL4how-24B, Scer\GAL4sr-md710 shotdsRNA.Scer\UAS lethal | larval stage | recessive shot65-2 shotunspecified lethal | recessive shot1 shotk03010 shotk03405 shotk04204 shotk05405 shotk05434b shotk05803 shotk05821 shotk06601 shotk10821 shotk15606 shotSF20 shotV168 Other Phenotypes Allele decreased cell number | somatic clone shot3 shot6 shot7 shot8 neuroanatomy defective shot2 shot3 shotk03010 shotk06601 neuroanatomy defective (with Df(2R)CX1) shot3 neuroanatomy defective (with shot2) shot3 neuroanatomy defective (with shot3) shot2 neuroanatomy defective | embryonic stage (with shot91k) shotHG25 shotSF20 neuroanatomy defective | embryonic stage (with shotel3) shotSF20 neuroanatomy defective | embryonic stage (with shotHG25) shot91k neuroanatomy defective | embryonic stage (with shotSF20) shot91k shotel3 neuroanatomy defective | embryonic stage | recessive shot3 shot91k shotel3 shotSF20 neuroanatomy defective | somatic clone shot3 shot6 shot7 shot8 paralytic | recessive shot91k shotel3 shotHG25 shotSF20 visible | somatic clone shot65-2 shotunspecified shotV104 shotV167 Phenotype manifest in Allele abdominal segmental nerve shot3 axon & motor neuron | embryonic stage shotk03405 axon & pCC neuron shot3 centrosome & ovary | germ-line clone shot3 chordotonal organ shot91k shotSF20 shotel3 chordotonal organ & axon | lateral shot1 shot2 shot3 chordotonal organ | embryonic stage | lateral shot2 shot3 shotk03010 shotk05460 shotk06601 dendrite & motor neuron | embryonic stage shotk03405 shotunspecified dendrite | dorsal | embryonic stage shot30 shotk03010 dorsal closure embryo shotV168 shotk03010 shotk03405 dorsal trunk primordium shot3 egg chamber & follicle cell | somatic clone shot3 embryonic/first instar larval cuticle shot91k shotHG25 shotSF20 shotel3 embryonic/first instar larval cuticle | dorsal shotV104 shotV167 embryonic/larval dorsal anastomosis shot3 embryonic/larval somatic muscle shot91k shotA405 shotHG25 shotSF20 shotV104 shotel3 embryonic epidermis shotA405 shotV104 shotk03405 embryonic proventriculus shotunspecified extended germ band embryo shotV168 shotk03010 shotk03405 imaginal disc shotk04204 intersegmental nerve shot2 shot3 larva shot65-2 shotunspecified larval mushroom body | somatic clone shot6 shot7 shot8 lateral trunk primordium shot3 longitudinal connective shot3 microtubule & ovary | germ-line clone shot3 muscle attachment site shotA405 shotV104 muscle attachment site | embryonic stage shot91k shotHG25 shotSF20 shotel3 mushroom body & axon shot1 shot3 mushroom body calyx | somatic clone shot3 neuromuscular junction | embryonic stage shot91k shotHG25 shotSF20 shotel3 nurse cell | ectopic | germline clone shot3 oocyte shot3 oocyte | germline clone shot3 ovary | germline clone shot3 presumptive embryonic/larval peripheral nervous system | dorsal shotk03010 proventriculus inner layer primordium shotunspecified proventriculus intermediate layer primordium shotunspecified RP1 neuron shot2 RP3 neuron shot2 shot91k shotSF20 shotel3 scolopidial dendrite | embryonic stage shot2 shot3 shotk06601 segmental nerve shot2 shot3 shot91k shotSF20 sensory neuron shot3 synapse & neuromuscular junction shot3 shotHG25 synapse & neuromuscular junction (with Df(2R)MK1) shotSF20 tendon cell, with Scer\GAL4how-24B, Scer\GAL4sr-md710 shotdsRNA.Scer\UAS tendon cell & actin filament, with Scer\GAL4how-24B, Scer\GAL4sr-md710 shotdsRNA.Scer\UAS tendon cell & microtubule, with Scer\GAL4how-24B, Scer\GAL4sr-md710 shotdsRNA.Scer\UAS tendon cell | embryonic stage 17 shotunspecified wing, with Scer\GAL4Bx-MS1096 shotdsRNA.Scer\UAS wing | somatic clone shot65-2 shotV104 shotV167 shotunspecified
Classical Alleles ( 64 )
For All Classical Alleles Show
Allele of shot Class Mutagen Stocks Known lesion shot3 loss of function allele diepoxybutane 3 -- shotk03010 P-element activity 2 Yes shotk03405 hypomorphic allele - genetic evidence P-element activity 2 Yes shotKG07812 2 -- shotSF20 loss of function allele ethyl methanesulfonate 2 -- shot5-HA-1201 1 -- shot5-HA-2785 1 -- shot65-2 X ray 1 -- shotc00857 piggyBac transposase 1 -- shotc01025 piggyBac transposase 1 -- shotc03591 1 -- shotc05431 piggyBac transposase 1 -- shotc07162 piggyBac transposase 1 -- shotd00747 P-element activity 1 -- shotd02359 P-element activity 1 -- shotd02801 P-element activity 1 -- shotd03296 P-element activity 1 -- shotd05068 P-element activity 1 -- shotd06144 P-element activity 1 -- shotd09913 P-element activity 1 -- shotDG29710 P-element activity 1 -- shotEY01491a P-element activity 1 -- shotEY15738 P-element activity 1 -- shotf06882 piggyBac transposase 1 -- shotf07764 piggyBac transposase 1 -- shotk04204 P-element activity 1 -- shotk06601 P-element activity 1 Yes shotk15606 P-element activity 1 -- shotKG02923 P-element activity 1 -- shotMI01617 1 -- shotMI03583 1 -- shotNP3076 1 -- shotNP3315 1 -- shotV104 X ray 1 Yes shot1 ethyl methanesulfonate 0 -- shot2 diepoxybutane 0 -- shot30 ethyl methanesulfonate 0 -- shot5-HA-3007 0 -- shot5 0 -- shot6 ethyl methanesulfonate 0 -- shot7 ethyl methanesulfonate 0 -- shot8 ethyl methanesulfonate 0 -- shot91k 0 -- shotA405 ethyl methanesulfonate 0 -- shotc03247 piggyBac transposase 0 -- shotCPTI001962 piggyBac transposase 0 Yes shotCPTI003653 piggyBac transposase 0 Yes shotd01259 P-element activity 0 -- shotd02828 0 -- shotd05163 P-element activity 0 -- shotd08375 P-element activity 0 -- shotel3 0 -- shotGS10458 0 Yes shotHG25 0 -- shotk05405 P-element activity 0 -- shotk05434b P-element activity 0 -- shotk05460 P-element activity 0 Yes shotk05803 P-element activity 0 -- shotk05821 P-element activity 0 -- shotk10817 0 -- shotk10821 P-element activity 0 -- shotunspecified X ray 0 -- shotV167 X ray 0 -- shotV168 X ray 0 --
Alleles Carried on Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Allele of shot Class Mutagen Stocks Known lesion shotLC.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes shotJF02971 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes shotLA-ΔEF-hand.Scer\UAS.T:Avic\GFP in vitro construct - deletion 1 Yes shotLA-ΔPlakin.Scer\UAS.T:Avic\GFP in vitro construct - deletion 1 Yes shotLA-Δrod1.Scer\UAS.T:Avic\GFP in vitro construct - deletion 1 Yes shotLA.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes shotLC-ΔGAS2.Scer\UAS.T:Avic\GFP in vitro construct - deletion 1 Yes shotActin.Plakin.Scer\UAS.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes shotCL.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes shotdsRNA.cRa in vitro construct - RNAi 0 Yes shotdsRNA.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes shotEF.GAS2.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes shotGD9507 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP in vitro construct - deletion 0 Yes shotLB.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes shotPlakin.Scer\UAS.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)6r35 (Prokop et al., 1998) Df(2R)BSC307 (Christensen and Cook, 2007.5.8) Df(2R)BSC383 (Christensen et al., 2007.10.29) Df(2R)CX1 (Walsh and Brown, 1998, Prokop et al., 1998, Spradling et al., 1999, Lee et al., 2000, BDGP Project Members, 1994-1999, Prout et al., 1997) Df(2R)MK1 (Gregory and Brown, 1998, Prokop et al., 1998) Df(2R)Su(z)2-1.a4 (Lee et al., 2000)
Duplicated in	Dp(2;3)6r35 (Prokop et al., 1998)
Not disrupted in	Df(2R)50C-38 (Prokop et al., 1998, BDGP Project Members, 1994-1999) Df(2R)50C-45 (BDGP Project Members, 1994-1999) Df(2R)50C-101 (Prokop et al., 1998, BDGP Project Members, 1994-1999) Df(2R)50C-102 (BDGP Project Members, 1994-1999) Df(2R)Su(z)2-1.a3 (Lee et al., 2000) Df(2R)vg-B (Prokop et al., 1998, BDGP Project Members, 1994-1999) Df(2R)vg-C (Prokop et al., 1998) Df(2R)vg-D (Prokop et al., 1998)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9507} NA P{TRiP.JF02971} NA P{UAS-C-shot.L-GFP} NA P{UAS-dskakRNA} NA P{UAS-shot.Actin.Plakin.TAP} NA P{UAS-shot.L(A)-GFP} NA P{UAS-shot.L(A)-ΔEF-hand-GFP} NA P{UAS-shot.L(A)-ΔGAS2-GFP} NA P{UAS-shot.L(A)-ΔPlakin-GFP} NA P{UAS-shot.L(A)-Δrod1-GFP} NA P{UAS-shot.L(B)-GFP} NA P{UAS-shot.L(C)-GFP} NA P{UAS-shot.L(C)-ΔGAS2-GFP} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}k05434a No P{lacW}l(2)SH0713SH0713 No P{lacW}mamk05821 No P{lacW}shotk03010 No P{lacW}shotk03405 No P{lacW}shotk04204 No P{lacW}shotk05405 No P{lacW}shotk05434b No P{lacW}shotk05460 No P{lacW}shotk05803 No P{lacW}shotk06601 No P{lacW}shotk10817 No P{lacW}shotk10821 No insertion of mobile activating element P{EPgy2}shotEY01491a NA P{EPgy2}shotEY15738 NA P{GSV6}shotGS10458 NA P{XP}shotd00747 NA P{XP}shotd01259 NA P{XP}shotd02359 NA P{XP}shotd02801 NA P{XP}shotd02828 NA P{XP}shotd03296 NA P{XP}shotd05068 NA P{XP}shotd05163 NA P{XP}shotd06144 NA P{XP}shotd06960 NA P{XP}shotd08375 NA P{XP}shotd09913 NA miscellaneous insertions Mi{MIC}shotMI01617 NA Mi{MIC}shotMI03583 NA P{RS5}shot5-HA-1201 NA P{RS5}shot5-HA-2785 NA P{RS5}shot5-HA-3007 NA P{SUPor-P}shotKG02923 NA P{SUPor-P}shotKG07812 NA P{wHy}shotDG29710 NA PBac{681.P.FSVS-1}shotCPTI001962 NA PBac{768.FSVS-0}shotCPTI003653 NA PBac{HpaI-GFP.A}YD0728 NA PBac{PB}shotc00857 NA PBac{PB}shotc01025 NA PBac{PB}shotc03247 NA PBac{PB}shotc03591 NA PBac{PB}shotc05431 NA PBac{PB}shotc07162 NA PBac{WH}shotf06882 NA PBac{WH}shotf07764 NA insertion of enhancer trap binary system P{GawB}OK307 No P{GawB}shotNP3076 No P{GawB}shotNP3315 No
Gene Ontology: Function, Process & Cellular Component ( 30 unique terms )
Terms Based on Experimental Evidence ( 16 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with exba (Lee et al., 2007)
Biological Process
CV term Evidence References
axon midline choice point recognition inferred from mutant phenotype (Lee et al., 2007) branch fusion, open tracheal system inferred from expression pattern (Lee et al., 2003) inferred from mutant phenotype (Lee and Kolodziej, 2002) dendrite morphogenesis inferred from mutant phenotype (Gao et al., 1999, Parrish et al., 2006, Bottenberg et al., 2009) lumen formation, open tracheal system inferred from mutant phenotype (Lee and Kolodziej, 2002) microtubule cytoskeleton organization inferred from mutant phenotype (Subramanian et al., 2003) mushroom body development inferred from mutant phenotype (Reuter et al., 2003) negative regulation of microtubule depolymerization inferred from mutant phenotype (Roper and Brown, 2004) oocyte fate determination inferred from mutant phenotype (Roper and Brown, 2004) open tracheal system development inferred from mutant phenotype (Lee and Kolodziej, 2002) regulation of axon extension inferred from mutant phenotype (Lee and Kolodziej, 2002)
Cellular Component
CV term Evidence References
adherens junction inferred from direct assay (Lee et al., 2003) astral microtubule inferred from direct assay (Slep et al., 2005) fusome inferred from direct assay (Roper and Brown, 2004) growth cone inferred from direct assay (Bottenberg et al., 2009) microtubule inferred from direct assay (Slep et al., 2005)
Terms Based on Predictions or Assertions ( 20 terms )
Molecular Function
CV term Evidence References
actin binding inferred from sequence or structural similarity (Roper et al., 2002) inferred from sequence or structural similarity with EMBL:AF150755 (FlyBase, 1992-) traceable author statement (Grevengoed and Peifer, 2003) calcium ion binding inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR011992 (FlyBase Curators et al., 2004-) cytoskeletal protein binding inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) microtubule binding inferred from sequence or structural similarity with EMBL:AF150755 (FlyBase, 1992-) traceable author statement (Roper et al., 2002, Grevengoed and Peifer, 2003)
Biological Process
CV term Evidence References
actin cytoskeleton organization traceable author statement (Grevengoed and Peifer, 2003) apposition of dorsal and ventral imaginal disc-derived wing surfaces traceable author statement (Roper et al., 2002) axonogenesis traceable author statement (Grueber and Jan, 2004, Jefferson et al., 2004, Roper et al., 2002, Grevengoed and Peifer, 2003) branch fusion, open tracheal system traceable author statement (Uv et al., 2003, Ghabrial et al., 2003) cell cycle arrest inferred from electronic annotation with InterPro:IPR003108 (FlyBase Curators et al., 2004-) dendrite morphogenesis traceable author statement (Grueber and Jan, 2004, Roper et al., 2002, Grevengoed and Peifer, 2003) determination of muscle attachment site traceable author statement (Bate et al., 1999) microtubule-based process inferred from sequence or structural similarity with EMBL:AF150755 (FlyBase, 1992-) non-traceable author statement (Brown, 2000, Brower, 2003) microtubule cytoskeleton organization traceable author statement (Grevengoed and Peifer, 2003) muscle attachment non-traceable author statement (Volk et al., 1995) traceable author statement (Jefferson et al., 2004, Roper et al., 2002) muscle organ development non-traceable author statement (Volk, 1995.4.17) open tracheal system development traceable author statement (Roper et al., 2002, Grevengoed and Peifer, 2003, Ghabrial et al., 2003) sensory organ development traceable author statement (Roper et al., 2002)
Cellular Component
CV term Evidence References
adherens junction traceable author statement (Jefferson et al., 2004) cytoskeleton non-traceable author statement (Brown, 2000) microtubule traceable author statement (Roper et al., 2002)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions shot allele Gene References shot65-2 mys (Prout et al., 1997) shotunspecified mys (Prout et al., 1997) unspecified exba (Lee et al., 2007) pbl (Gregory et al., 2007) robo (Lee et al., 2007) sli (Lee et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62296 DroID - A comprehensive database of gene and protein interactions. • FBgn0013733 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36542
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11157 Dere\GG22462 Dmoj\GI18447 Dper\GL17079 Dpse\GA24356 Dvir\GJ21532 Dwil\GK17953 Dyak\GE13333
Curated drosophilid orthologs	Dpse\GA14781
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL002829-PA Aedes aegypti (mosquito) AGAP011396-PA Anopheles gambiae (mosquito) XP_001949103.1 Acyrthosiphon pisum (pea aphid) 118484 Branchiostoma floridae (lancelet) ENSBTAP00000033188 Bos taurus (bovine) CE35152 Caenorhabditis elegans (worm) ENSCAFP00000004683 Canis familiaris (dog) CPIJ003239 Culex pipens (mosquito) ENSCPOP00000009509 Cavia porcellus (guinea pig) 217663 Capitella spl (polychaete worm) 313136 Daphnia pulex (daphnia) ENSDARP00000085102 Danio rerio (zebra fish) ENSECAP00000015717 Equus caballus (horse) ENSGACP00000008248 Gymnopilus aculeatus (mushroom) ENSGALP00000026214 Gallus gallus (chicken) ENSP00000359805 Homo sapiens (human) 233112 Lottia gigantea (owl limpet) ENSMODP00000023407 Monodelphis domestica (gray opossum) ENSMMUP00000001071 Macaca mulatta (rhesus monkey) ENSMUSP00000095392 Mus musculus (house mouse) XP_001606812.1 Nasonia vitripennis (wasp) ENSOANP00000015870 Ornithorynchus anatinus (platypus) ENSORLP00000012626 Oryzias latipes (japanese killifish) PHUM000721-PA Pediculus humanus (human body louse) ENSPPYP00000018723 Pongo pygmaeus (orangutan) ENSPTRP00000031270 Pan troglodytes (chimpanzee) ENSRNOP00000021951 Rattus norvegicus (norway rat) XP_001194617.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001195703.1 Strongylocentrotus purpuratus (purple sea urchin) 58289 Trichoplax adhaerens (metazoa) XP_973767.2 Tribolium castaneum (red flour beetle) ENSTNIP00000009790 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000039658 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000016061 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB18031 NV18329 LH25678 LH17534 LH17533 FBgn0231014 FBgn0219952 FBgn0208652 FBgn0197016 FBgn0197015 FBgn0175130 JGI_V11_313136 JGI_V11_302923 FBgn0245753 FBgn0154683 FBgn0141186 FBgn0130416 FBgn0114633 FBgn0088197 CPIJ003239 BGIBMGA004416 BGIBMGA004415 BGIBMGA004414 ACYPI005144 GB17469 AGAP011396 AAEL002829 PB26682 PB26684 PHUM227910 GLEAN_09077
Stocks & Reagents
Stocks Listed in FlyBase ( 47 )
Harvard	- P{XP}shotd00747 - P{XP}shotd02359 - P{XP}shotd02801 - P{XP}shotd03296 - P{XP}shotd05068 - P{XP}shotd06144 - P{XP}shotd09913 - PBac{PB}shotc00857 - PBac{PB}shotc01025 - PBac{PB}shotc03591 - PBac{PB}shotc05431 - PBac{PB}shotc07162
	More stocks available...
Genomic Clones ( 2 )
	BACR06M19 BACR16G12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 100 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP15341 LD39385
Other clones	MIP02579 MIP02679
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT26739 AT31807 GH05758 GH13829 GH13853 GH23032 GH23611 GH23640 GH27822 GM15148 HL03526 LD23618 LP01655 LP10280 RE12393 RH02041 RH04810 RH04851 RH05833 RH08030 RH15080 RH18014 RH35086 RH39374 RH42109 RH43122 RH52737 RH55468 RH62408 RH63639 RH64147 RH64663 RH66446 RH74613 SD17408 SD19937
Other clones	1412 33206 36503 41558 70943 72194 74821 106131 118334 122885 154434 158160 164552 175149 191633 193467 214279.26 214343.24 214505.17 214533.24 568892 569509 569686 CK01.34D.G5 CK01.41B.B8 CK01.66B.F8 CK01.133C.F6 EK029527 EK035540 EK053856 EK101355 EK122516 EK143564 EK147530 EK154531 EK200912 EK205203 EK206050 EK234108 EK237409 EK248227 EK258154 EK261048 EK274346 EK295047 EN14133 EN15035 EN15410 EP09858 EP18561 EP19620 ESG018b.E21.1_G08 FGA002H02 IP02952 LD40220 RP004162580 RP004198360 RP004524804 RP004525689 RP004545174
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013733 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36542
Antibody Information
	polyclonal (Strumpf and Volk, 1998, Bottenberg et al., 2009, Gregory and Brown, 1998)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: shot CG18076 (Milshina, 1999.11) Source for identity of: shot CG18076 (Zhao, 1999.11)
Source for database merge of	Source for merge of: kak grv (Brown and Volk, 1998.4.21) Source for merge of: kak l(2)CA4 (Prokop et al., 1998) Source for merge of: shot CG18637 (Gregory and Brown, 1998, FlyBase, 1996-) Source for merge of: shot l(2)k04204 l(2)k15606 l(2)k05821 l(2)k05434 l(2)k10821 (Gao et al., 1999)
Additional comments	Source for merge of shot CG18637 was sequence comparison (date:001104). (Gregory and Brown, 1998, FlyBase, 1996-)
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. shot RNAi causes routing defects, dorsal overextension, and a reduction in lateral branching of dorsally extended primary dendrites of class I da neurons. (Parrish et al., 2006) Association of the shot protein with microtubules requires Eb1. (Slep et al., 2005) shot is required for ectodermal cell movement during development of the proventriculus. (Fuss et al., 2004) shot organizes microtubules during oocyte specification. (Roper and Brown, 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Identification: 3 alleles of shot have been identified in a screen to isolate genes required for normal neuronal morphogenesis in larval mushroom body neurons. (Reuter et al., 2003) shot recruits Eb1/Apc and promotes microtubule assembly at the muscle-tendon junction. (Subramanian et al., 2003) The F-actin- and microtubule-binding domains of shot are required in the same molecule for axon extension. (Lee and Kolodziej, 2002) shot is required for sensory and motor axons to reach their targets in the embryo. (Lee et al., 2000) shot has a function in dendritic development. (Gao et al., 1999) shot is required in mushroom body neurons for the extension, fasciculation and guidance of their axons. (Lee and Luo, 1999) 'groovin' was initially defined as an antigen recognised by a monoclonal antibody. The antibody identified a shot cDNA and immunoprecipitates the shot product, although the 'groovin' antibody still stains embryos deficient for the shot gene. (Brown and Volk, 1998.4.21) Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology. (Roch et al., 1998) shot is essential for muscle-dependent tendon cell differentiation in the developing embryo. (Strumpf and Volk, 1998) Identification: Genetic screen for mutations that produce blisters in somatic wing clones. 19 "kopupu" alleles have been isolated. (Walsh and Brown, 1998) Mutations may affect cell adhesion or muscle function at hatching. (Prout et al., 1997) Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones. (Prout et al., 1997) Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones. 5 alleles of shot have been isolated. (Prout et al., 1997) Mutations in 12 complementation groups differentially affect lateral chordotonal axon growth, fasciculation or ventral orientation. Mutations in axe, gui and shot result in shorter, but fasciculated and ventrally directed axon bundles. (Kolodziej et al., 1995) shot is expressed in the ectoderm at the future muscle attachment sites and may be required for apodeme differentiation to enable the correct targeting by the approaching myotubule. (Volk et al., 1995) Antisense experiments suggest that shot is important in a correct apodeme function to induce appropriate muscle pattern. (Volk, 1995.4.17) The 'groovin' antigen was identified in a monoclonal antibody screen and is expressed along the segmental groove of developing embryos. (Volk and Vijay Raghavan, 1994) Identification: Screen for mutations affecting neuromuscular connectivity, using an antibody to Fas2 to see motoneurons and axons. (van Vactor et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079009 NM_166015 NM_166016 NM_166017 NM_166018 NM_166019 NM_001144185 NM_001144186 NM_001144187 NM_001144188 NM_001144189 NM_001169676 NM_001169677 NM_001169678 NM_001169679 RefSeq (Proteins) • NP_523733 NP_725335 NP_725336 NP_725337 NP_725338 NP_725339 NP_001137657 NP_001137658 NP_001137659 NP_001137660 NP_001137661 NP_001163147 NP_001163148 NP_001163149 NP_001163150 DDBJ / EMBL / GenBank • AAF24340 AAF24341 AAF24342 AAF24343 AAF58317 AAF58319 AAF58320 AAG22268 AAM52693 AAM68561 AAM68562 ACL83111 ACL83112 ACL83113 ACL83114 ACL83115 ACZ94419 ACZ94420 ACZ94421 ACZ94422 AF200422 AF200423 AF200424 AF200425 AI519702 AJ011924 AJ011925 AQ025736 AQ025944 AQ034139 AW943466 AY122181 BH615092 BZ665554 CAA09869 CAA09870 CAA70581 CZ466770 CZ466819 CZ468531 CZ469968 CZ471298 CZ471781 CZ472126 CZ472770 CZ473003 CZ473275 CZ474300 CZ474301 CZ474358 CZ474940 CZ476202 CZ477008 CZ489617 CZ490504 Y09430 Entrez Gene - A searchable database of RefSeq genes. • 36542 UniProtKB/TrEMBL • A1Z9I8 A1Z9I9 A1Z9J0 A1Z9J1 A1Z9J2 A1Z9J3 B7YZG2 B7YZG3 B7YZG4 B7YZG5 B7YZG6 E1JH62 E1JH63 E1JH64 E1JH65 O77291 O96936 Q7KAJ2 Q7KJN8 Q7KJN9 Q8MR19 Q9U479
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Spectrin/alpha-actinin (IPR018159) EF-HAND 2 (IPR018249) EF-Hand 1, calcium-binding site (IPR018247) Plectin repeat (IPR001101) Actinin-type, actin-binding, conserved site (IPR001589) Calponin homology domain (IPR001715) Spectrin repeat (IPR002017) Calcium-binding EF-hand (IPR002048) Growth-arrest-specific protein 2 domain (IPR003108) EF-hand-like domain (IPR011992) Src homology-3 domain (IPR001452)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62296 DroID - A comprehensive database of gene and protein interactions. • FBgn0013733 DRSC - Results from RNAi screens. • FBgn0013733 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013733 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18076-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013733 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36542 InParanoid A subset of ortholog calls from InParanoid. • AAEL002829-PA Aedes aegypti (mosquito) AGAP011396-PA Anopheles gambiae (mosquito) XP_001949103.1 Acyrthosiphon pisum (pea aphid) 118484 Branchiostoma floridae (lancelet) ENSBTAP00000033188 Bos taurus (bovine) CE35152 Caenorhabditis elegans (worm) ENSCAFP00000004683 Canis familiaris (dog) CPIJ003239 Culex pipens (mosquito) ENSCPOP00000009509 Cavia porcellus (guinea pig) 217663 Capitella spl (polychaete worm) 313136 Daphnia pulex (daphnia) ENSDARP00000085102 Danio rerio (zebra fish) ENSECAP00000015717 Equus caballus (horse) ENSGACP00000008248 Gymnopilus aculeatus (mushroom) ENSGALP00000026214 Gallus gallus (chicken) ENSP00000359805 Homo sapiens (human) 233112 Lottia gigantea (owl limpet) ENSMODP00000023407 Monodelphis domestica (gray opossum) ENSMMUP00000001071 Macaca mulatta (rhesus monkey) ENSMUSP00000095392 Mus musculus (house mouse) XP_001606812.1 Nasonia vitripennis (wasp) ENSOANP00000015870 Ornithorynchus anatinus (platypus) ENSORLP00000012626 Oryzias latipes (japanese killifish) PHUM000721-PA Pediculus humanus (human body louse) ENSPPYP00000018723 Pongo pygmaeus (orangutan) ENSPTRP00000031270 Pan troglodytes (chimpanzee) ENSRNOP00000021951 Rattus norvegicus (norway rat) XP_001194617.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001195703.1 Strongylocentrotus purpuratus (purple sea urchin) 58289 Trichoplax adhaerens (metazoa) XP_973767.2 Tribolium castaneum (red flour beetle) ENSTNIP00000009790 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000039658 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000016061 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/kakapo1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36542 modMine - Data generated by the modENCODE project. • FBgn0013733 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB18031 NV18329 LH25678 LH17534 LH17533 FBgn0231014 FBgn0219952 FBgn0208652 FBgn0197016 FBgn0197015 FBgn0175130 JGI_V11_313136 JGI_V11_302923 FBgn0245753 FBgn0154683 FBgn0141186 FBgn0130416 FBgn0114633 FBgn0088197 CPIJ003239 BGIBMGA004416 BGIBMGA004415 BGIBMGA004414 ACYPI005144 GB17469 AGAP011396 AAEL002829 PB26682 PB26684 PHUM227910 GLEAN_09077
Synonyms & Secondary IDs ( 31 )
Reported As
Symbol Synonym	42/4 (Roch et al., 1998) CG18076 (Ramet et al., 2002, Goldstein and Gunawardena, 2000, Goldstein and Gunawardena, 2000, Quinones-Coello, 2007, Quinones-Coello, 2007, Perkins et al., 2009, Philipps et al., 2004, Cammarato et al., 2011, Lowery et al., 2010) CG18637   grv (Strumpf, 1996.11.7, Volk, 1995.4.17, ) Grv (Fuerstenberg and Giniger, 1998, Vorbruggen and Jackle, 1997) kak (Fuss et al., 2004, Grueber and Jan, 2004, Brown, 1998.10.9, Brown, 1998.10.9, Gao et al., 1999, Prokop, 1999, Gregory and Brown, 1999, Strumpf and Volk, 1998, Prokop et al., 1998, Gregory and Brown, 1998, Brown and Volk, 1998.4.21, Prout et al., 1997, Prout et al., 1997, Bokel et al., 2005) kop (Walsh and Brown, 1998) l(2)CA4   l(2)k03010 (Spradling et al., 1999, ) l(2)k04204 (Spradling et al., 1999, Gregory and Brown, 1998, BDGP Project Members, 1994-1999, ) l(2)k05434 (BDGP Project Members, 1994-1999, ) l(2)k05821 (BDGP Project Members, 1994-1999, ) l(2)k10821 (BDGP Project Members, 1994-1999, ) l(2)k15606   shortstop/kakapo (Leung et al., 2001) Shot (Lee and Kolodziej, 2000, Slep et al., 2005, Applewhite et al., 2010, Pines and Röper, 2007, Alves-Silva et al., 2007, Sanchez-Soriano et al., 2007, Olguín et al., 2011, Lee et al., 2007, Rogers et al., 2008, Sanchez-Soriano et al., 2009, Wayburn and Volk, 2009, Pines et al., 2010, Klein et al., 2010, Djiane et al., 2011, Olguín et al., 2011) shot (Parrish et al., 2006, Gregory et al., 2007, Christensen and Cook, 2007.5.8, Bokel et al., 2005, Christensen et al., 2007.10.29, Gao and Bogert, 2003, Quinones-Coello, 2007, Quinones-Coello, 2007, Lee et al., 2007, Bakal et al., 2007, Kaltenbach et al., 2007, Sandmann et al., 2006, Roper, 2007, Jiang and Edgar, 2009, Alves-Silva et al., 2008, Dworkin et al., 2009, Philipps et al., 2004, Bottenberg et al., 2009, Gilsohn and Volk, 2010, Magalhaes et al., 2007, Sánchez-Soriano et al., 2010, Carmon et al., 2010, Dilks and DiNardo, 2010, Beltran et al., 2007, Wasbrough et al., 2010, Lowery et al., 2010) unnamed (Spradling, 1999.12.9)
Name Synonym	groovin (Laayouni et al., 2000, Bate et al., 1999, Volk, 1995.4.17, Volk et al., 1995, Volk and Vijay Raghavan, 1994, ) kakapo (Lohr et al., 2002, Ackley and Jin, 2004, Jefferson et al., 2004, Jan and Yan, 2003, Acebes and Ferrus, 2000, Gao and Bogert, 2003, Jan and Jan, 2001, Matus and Shepherd, 2000, Brown, 2000, Brown et al., 2000, Goldstein and Gunawardena, 2000, Gao et al., 1999, Prout et al., 1997, Jiang and Edgar, 2009, Carmon et al., 2010) Kakapo (Subramanian and Volk, 2002, Goldstein and Gunawardena, 2000, Volk, 1999, Slep et al., 2005) kakapoo (Unluer et al., 2003) kopupu (Walsh and Brown, 1998) shortshop (Dilks and DiNardo, 2008) shortstop (Jefferson et al., 2004, Gilsohn and Volk, 2010) short-stop (Unluer et al., 2003) short stop (van Vactor et al., 1993, Parrish et al., 2006, Volohonsky et al., 2007, Kaltenbach et al., 2007, Jiang and Edgar, 2009, Shalaby et al., 2009, Sánchez-Soriano et al., 2010, Carmon et al., 2010, Dilks and DiNardo, 2010, Cammarato et al., 2011, Lowery et al., 2010) Shortstop (Slep et al., 2005, Rogers et al., 2008, Wayburn and Volk, 2009, Klein et al., 2010) Short Stop (Crews and Brenman, 2006, Applewhite et al., 2010, Loer et al., 2008) Short stop (Lee et al., 2007, Alves-Silva et al., 2008, Sanchez-Soriano et al., 2009, Bottenberg et al., 2009) Spectraplakin (Lewandowski et al., 2010)
Secondary FlyBase IDs
	FBgn0014183 FBgn0015972 FBgn0020296 FBgn0021837 FBgn0021937 FBgn0022152 FBgn0022170 FBgn0022208 FBgn0022230
References ( 174 )
Generate a list of	All references relating to this gene ( 174 )
List References by type	Research paper  ( 72 ) Supplementary material  ( 15 ) Review  ( 30 ) Personal communication to FlyBase  ( 18 ) Abstract  ( 25 ) FlyBase analysis  ( 3 ) Conference report  ( 1 ) DNA/RNA sequence record  ( 4 ) Computer file  ( 4 ) Curated genome annotation  ( 2 )
Recent research papers ( 12 )
	Djiane et al., 2011, J. Cell Biol. 192(1): 189--200 Su(dx) E3 ubiquitin ligase-dependent and -independent functions of Polychaetoid, the Drosophila ZO-1 homologue. [FBrf0212737] Mui et al., 2011, PLoS ONE 6(10): e25965 Role of spectraplakin in Drosophila photoreceptor morphogenesis. [FBrf0216481] Olguín et al., 2011, Curr. Biol. 21(3): 236--242 Intertissue mechanical stress affects frizzled-mediated planar cell polarity in the Drosophila notum epidermis. [FBrf0212947] Applewhite et al., 2010, Mol. Biol. Cell 21(10): 1714--1724 The spectraplakin Short stop is an actin-microtubule cross-linker that contributes to organization of the microtubule network. [FBrf0210771] Carmon et al., 2010, Fly 4(2): 117--127 dumpy interacts with a large number of genes in the developing wing of Drosophila melanogaster. [FBrf0211059] Dilks and DiNardo, 2010, Development 137(8): 1395--1404 Non-cell-autonomous control of denticle diversity in the Drosophila embryo. [FBrf0210398] Gilsohn and Volk, 2010, Development 137(5): 785--794 Slowdown promotes muscle integrity by modulating integrin-mediated adhesion at the myotendinous junction. [FBrf0209925] Klein et al., 2010, Dev. Biol. 337(2): 458--470 The proprioceptive and contractile systems in Drosophila are both patterned by the EGR family transcription factor Stripe. [FBrf0209777] Lewandowski et al., 2010, Dev. Biol. 339(2): 307--319 Mago Nashi, Tsunagi/Y14, and Ranshi form a complex that influences oocyte differentiation in Drosophila melanogaster. [FBrf0210119] Lowery et al., 2010, Genetics 185(4): 1311--1325 Parallel Genetic and Proteomic Screens Identify Msps as a CLASP-Abl Pathway Interactor in Drosophila. [FBrf0211499] Pines et al., 2010, Development 137(6): 913--922 The cytolinker Pigs is a direct target and a negative regulator of Notch signalling. [FBrf0210124] Sánchez-Soriano et al., 2010, Dev. Neurobiol. 70(1): 58--71 Drosophila growth cones: a genetically tractable platform for the analysis of axonal growth dynamics. [FBrf0209638] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...