FlyBase Gene Report: Dmel\Awh

General Information

Symbol

Dmel\Awh

Species

D. melanogaster

Name

Arrowhead

Annotation Symbol

CG1072

Feature Type

protein_coding_gene

FlyBase ID

FBgn0013751

Gene Model Status

Current

Stock Availability

36 publicly available

Gene Summary

Arrowhead (Awh) encodes a LIM-homeodomain transcription factor that plays important roles in wing and abdominal development. [Date last reviewed: 2018-09-13] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

l(3)63Ea

Key Links

Genomic Location

Cytogenetic map

63F4-63F4

Sequence location

3L:3,846,206..3,861,692 [+]

Recombination map

3-9

RefSeq locus

NT_037436 REGION:3846206..3861692

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables DNA binding

inferred from electronic annotation with InterPro:IPR001356

(InterPro Project Members, 2004-)

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN004680160

(GO Reference Genome Project, 2011-)

enables DNA-binding transcription factor activity

inferred from sequence or structural similarity with UniProtKB:O60663

(Gene Ontology Curators, 2002-)

enables RNA polymerase II transcription regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN004680160

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in compound eye development

inferred from mutant phenotype

(Roignant et al., 2010)

involved_in imaginal disc development

inferred from mutant phenotype

(Curtiss and Heilig, 1997)

involved_in regulation of gene expression

inferred from mutant phenotype

(Roignant et al., 2010)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in neuron differentiation

inferred from biological aspect of ancestor with PANTHER:PTN000654232

(GO Reference Genome Project, 2011-)

involved_in regulation of DNA-templated transcription

inferred from sequence or structural similarity with UniProtKB:O60663

(Gene Ontology Curators, 2002-)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN004680160

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000654232

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred from sequence or structural similarity with UniProtKB:O60663

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

LIM HOMEOBOX TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Arrowhead (Awh) encodes a LIM-homeodomain transcription factor that plays important roles in wing and abdominal development. [Date last reviewed: 2018-09-13]

Gene Group (FlyBase)

LIM HOMEOBOX TRANSCRIPTION FACTORS -: LIM homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. They have two N-terminal LIM domains (cysteine-rich, double-zinc finger motifs) and a centrally located sequence-specific DNA binding homeodomain. (Adapted from PMID:24676471 and FBrf0125444).

Protein Function (UniProtKB)

Probable transcription factor. Required for the establishment of a subset of imaginal tissues: the abdominal histoblasts and the salivary gland imaginal rings.

(UniProt, Q8IRC7)

Summary (Interactive Fly)

LIM domains and LIM homeodomain - requirement for proliferation of histoblast and imaginal ring tissue - along with Lim1, uses Chip as a co-factor to prevent retinal differentiation in regions of the eye disc destined to become ventral head tissue

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Awh for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8IRC7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216884.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Alternative translation stop created by use of multiphasic reading frames within coding region.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Awh-RA

FBtr0073155

NM_079183

2039

214

Awh-RB

FBtr0073156

NM_168042

2104

275

Awh-RC

FBtr0330070

NM_001274450

2104

287

Awh-RD

FBtr0330071

NM_001274451

2092

271

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Awh-PA

FBpp0073013

24.3

214

8.36

NP_523907

AAF47800

Awh-PB

FBpp0073014

31.0

275

7.82

FBpp0303103

32.3

287

7.95

FBpp0303104

30.6

271

7.53

M9PE75

NP_001261380

AGB94075

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Awh using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Awh_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.91

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

central brain anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

hypopharynx anlage

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium