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Gene Dmel\CASK

General Information
Symbol	Dmel\CASK	Species	D. melanogaster
Name	CASK ortholog	Annotation symbol	CG6703
Feature type	protein_coding_gene	FlyBase ID	FBgn0013759
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	caki, Cmg, CamGUK, CG13412
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	93F10-93F12	Sequence location	3R:17,609,122..17,648,396 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene CASK ortholog is referred to in FlyBase by the symbol Dmel\CASK (CG6703, FBgn0013759). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: calmodulin-dependent protein kinase activity. There is experimental evidence that it is involved in the biological process: regulation of cell shape; adult walking behavior; regulation of neurotransmitter secretion; lateral inhibition; adult locomotory behavior; cell adhesion; male courtship behavior. 21 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; chaeta. It has 7 annotated transcripts and 7 annotated polypeptides. Protein features are: Guanylate kinase; Guanylate kinase, conserved site; Guanylate kinase/L-type calcium channel; L27; L27, C-terminal; PDZ domain; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Src homology-3 domain; Variant SH3. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during early larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1628985_a_at completely aligns to an exonic region common to each of the 6 FlyBase-annotated transcript isoforms of Caki. Gene sequence location is 3R:17609122..17648396.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 93F10-93F12   Limits computationally determined from genome sequence between P{PZ}tsl00617 and P{lacW}howj5B5&P{EP}EP738
Experimentally Determined Cytological Location
Cytogenetic map Notes References 93F-93F   (determined by in situ hybridisation)   (Dimitratos et al., 1997) 93F-93F     (Dimitratos et al., 1997) 93E-93E   (determined by in situ hybridisation)   (Martin and Ollo, 1996) 93F6-93F14     (Martin et al., 1994) 93F-93F   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones. Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000)
Experimentally Determined Recombination Data
Location	3-
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CASK for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	gene_with_stop_codon_read_through ; SO:0000697 Gene model reviewed during 5.44 Stop-codon suppression (UGA) postulated; FBrf0216884. Gene model reviewed during 5.47 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CASK-RA FBtr0084162  NM_169970   4112   591 CASK-RB FBtr0084161  NM_079717   4128   898 CASK-RD FBtr0112872  NM_001104392   4017   591 CASK-RE FBtr0112873  NM_001104393   2586   469 CASK-RF FBtr0301327  NM_001170210   6544   916 CASK-RG FBtr0301328  NM_169971   4071   609 CASK-RH FBtr0330368  NM_001275882   4128   929
Additional Transcript Data & Comments
Reported size (kB)	7.0, 4.0 (northern blot) (Martin and Ollo, 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CASK-PA   FBpp0083560   65.2   591   6.87     NP_732661   AAF55921 CASK-PB   FBpp0083559   100.9   898   6.20     NP_524441   AAF55922 CASK-PD   FBpp0111785   65.2   591   6.87     NP_001097862   ABW08723 CASK-PE   FBpp0111786   53.3   469   6.47     NP_001097863   ABW08724 CASK-PF   FBpp0290542   102.5   916   6.08     NP_001163681   ACZ94977 CASK-PG   FBpp0290543   66.7   609   6.67     NP_732662   AAF55920 CASK-PH   FBpp0303394   104.3   929   5.75     NP_001262811   AGB96191
Additional Polypeptide Data & Comments
Reported size (kDa)	571 (aa); 63 (kD predicted) (Martin and Ollo, 1996)
Comments	Constitutively expressed CASK protein was shown to be enzymatically active in a cotransfection assay in rat pituitary cells. Sequence analysis indicates that CASK may be a member of a new branch of CaM kinases. (Martin and Ollo, 1996)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Src homology-3 domain (IPR001452) PDZ domain (IPR001478) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) L27 (IPR004172) Guanylate kinase (IPR008144) Guanylate kinase/L-type calcium channel (IPR008145) Protein kinase-like domain (IPR011009) Variant SH3 (IPR011511) L27, C-terminal (IPR014775) Guanylate kinase, conserved site (IPR020590) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA539863   AA567334   AA567506   AE014297 AAF55920   AE014297 AAF55921   AE014297 AAF55922   AE014297 ABW08723   AE014297 ABW08724   AE014297 ACZ94977   AE014297 AGB96191   AI541658   AI546148   AY094916 AAM11269   BG637648   BI162546   BI163609   BI163639   BI164160   BI166702   BI168732   BI170697   BI230113   BI242137   BI354416   BI356285   BI363256   BI586211   BI592788   BI616562   BP541431   BP546314   BP555009   BP555010   BT003532 AAO39536   BT003550 AAO39554   BT044550   BT046172   BT072975   CL527975   CO179212   CO190655   CO262607   CO263389   CO263903   CO264378   CO268065   CO269232   CO269236   CO271115   CO272482   CO276867   CO283568   CO287909   CO289296   CO294209   CO294673   CO295457   CO297098   CO300745   CO303833   CO304472   CO309059   CO309541   CO313938   CO315565   CO316387   CO318542   CO318973   CO319533   CO319824   CO321489   CO323272   CO327457   CO334113   CO335763   CO342161   CO344393   CZ483474   CZ483475   CZ489912   CZ489913   EC053546   EC053551   EC054031   EC054039   EC054528   EC055349   EC055930   EC056205   EC056452   EC056560   EC059296   EC068017   EC070785   EC071098   EC071193   EC071844   EC076051   EC077734   EC077833   EC078440   EC081992   EC082047   EC086118   EC087110   EC089746   EC195012   EC196082   EC198386   EC199967   EC202759   EC206965   EC207906   EC213284   EC218940   EC220022   EC220566   EC223403   EC227473   EC228908   EC231082   EC231151   EC231552   EC231912   EC236062   EC236867   EC237638   EC239204   EC241982   EC242592   EC247287   EC248589   EC248653   EC250415   EC253395   EC254149   EC257198   EC261363   EV599983   GH941288   GH942152   GH944606   GH950327   GH951163   GH951738   GH953334   GH956626   GH962021   GH964712   GH968247   GH968603   U53190 AAC80169   X94264 CAA63940
UniProtKB/Swiss-Prot	Q24210
UniProtKB/TrEMBL	A8JR71 A8JR72 E1JIS7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5 -- 7   somatic cell   (Lopes et al., 2001) embryonic stage 7   cephalic furrow   (Lopes et al., 2001) embryonic stage 8 -- 9   ventral neurectoderm   (Lopes et al., 2001) embryonic stage 8 -- 12   germ band   (Lopes et al., 2001) epithelial furrow   (Lopes et al., 2001) amnioproctodeal invagination   (Lopes et al., 2001) posterior midgut primordium   (Lopes et al., 2001) anterior midgut primordium   (Lopes et al., 2001) embryonic stage 10 -- 12   ventral nerve cord primordium   (Lopes et al., 2001) Comment:> embryonic stage 13 -- 14   midgut primordium   (Lopes et al., 2001) embryonic stage 13 -- third instar larval stage   embryonic/larval central nervous system   (Lopes et al., 2001) embryonic stage 17   embryonic/larval Malpighian tubule   (Lopes et al., 2001) pupal stage -- adult stage   supraesophageal ganglion   (Lopes et al., 2001) optic lobe   (Lopes et al., 2001) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 8 -- 12   posterior midgut primordium   (Martin and Ollo, 1996) anterior midgut primordium   (Martin and Ollo, 1996) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) embryonic central brain glial cell   (Fisher et al., 2012) embryonic central brain surface glia   (Fisher et al., 2012) embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) midline primordium   (Fisher et al., 2012) midline ventral glial cell   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) procephalic neuroblast   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral midline   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Martin and Ollo, 1996) embryonic stage 9 -- 17   presumptive embryonic/larval central nervous system | presumptive   (Martin and Ollo, 1996) embryonic stage 11   neuroblast   (Martin and Ollo, 1996) embryonic stage 12 -- 17     (Martin and Ollo, 1996) Comment:reference states 8-24 hr AEL embryonic stage 14   embryonic/larval hindgut   (Martin and Ollo, 1996) Malpighian tubule   (Martin and Ollo, 1996) larval stage   embryonic/larval central nervous system   (Martin and Ollo, 1996) pupal stage   central nervous system   (Martin and Ollo, 1996) adult stage     (Martin and Ollo, 1996) adult head   (Martin and Ollo, 1996) oogenesis & adult stage | female   ovary   (Martin and Ollo, 1996) adult stage & oogenesis   nurse cell   (Martin and Ollo, 1996) adult stage & oogenesis stage S10B   polar follicle cell   (Martin and Ollo, 1996)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference adult stage   lamina   (Martin and Ollo, 1996) optic lobe   (Martin and Ollo, 1996) adult brain   (Martin and Ollo, 1996) lobula   (Martin and Ollo, 1996) lobula plate   (Martin and Ollo, 1996) medulla   (Martin and Ollo, 1996)
Additional Descriptive Data
	CASK protein is expressed in the optic lobe, the neuropil of the medulla, the lobula, and the lobula plate, while weaker expression is seen in the lamina. Expression is also detected in the central brain. (Martin and Ollo, 1996)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    581.275 Larval Midgut    180.3 Larval Hindgut    213.6 Larval Malpighian Tubules    201.5 Larval Fat Body    89.9 Larval Salivary Gland    107 Larval Trachea    200.9 Larval Carcass    142.325 Adult Head    300.1 Adult Eye    547.2 Adult Brain    1274.3 Adult Thoracic-Abdominal Ganglion    1237.3 Adult Crop    244.1 Adult Midgut    152.5 Adult Hindgut    260.6 Adult Malpighian Tubules    169.8 Adult Fat Body    69.3 Adult Salivary Gland    242.9 Adult Heart    141.45 Adult VirginFemale Spermatheca    302 Adult InseminatedFemale Spermatheca    288.4 Adult Ovary    326 Adult Testis    22.1 Adult Male Accessory Gland    128 Adult Carcass    83.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (581.275) Larval Midgut    180.3 Larval Hindgut    213.6 Larval Malpighian Tubules    201.5 Larval Fat Body    89.9 Larval Salivary Gland    107 Larval Trachea    200.9 Larval Carcass    142.325 Adult Head    300.1 Adult Eye  (547.2) Adult Brain  (1274.3) Adult Thoracic-Abdominal Ganglion  (1237.3) Adult Crop    244.1 Adult Midgut    152.5 Adult Hindgut    260.6 Adult Malpighian Tubules    169.8 Adult Fat Body    69.3 Adult Salivary Gland    242.9 Adult Heart    141.45 Adult VirginFemale Spermatheca    302 Adult InseminatedFemale Spermatheca    288.4 Adult Ovary    326 Adult Testis    22.1 Adult Male Accessory Gland    128 Adult Carcass    83.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    581.275 Larval Midgut    180.3 Larval Hindgut    213.6 Larval Malpighian Tubules    201.5 Larval Fat Body    89.9 Larval Salivary Gland    107 Larval Trachea    200.9 Larval Carcass    142.325 Adult Head    300.1 Adult Eye    547.2 Adult Brain  (1274.3) Adult Thoracic-Abdominal Ganglion  (1237.3) Adult Crop    244.1 Adult Midgut    152.5 Adult Hindgut    260.6 Adult Malpighian Tubules    169.8 Adult Fat Body    69.3 Adult Salivary Gland    242.9 Adult Heart    141.45 Adult VirginFemale Spermatheca    302 Adult InseminatedFemale Spermatheca    288.4 Adult Ovary    326 Adult Testis    22.1 Adult Male Accessory Gland    128 Adult Carcass    83.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    581.275 Larval Midgut    180.3 Larval Hindgut    213.6 Larval Malpighian Tubules    201.5 Larval Fat Body    89.9 Larval Salivary Gland    107 Larval Trachea    200.9 Larval Carcass    142.325 Adult Head    300.1 Adult Eye    547.2 Adult Brain    1274.3 Adult Thoracic-Abdominal Ganglion    1237.3 Adult Crop    244.1 Adult Midgut    152.5 Adult Hindgut    260.6 Adult Malpighian Tubules    169.8 Adult Fat Body    69.3 Adult Salivary Gland    242.9 Adult Heart    141.45 Adult VirginFemale Spermatheca    302 Adult InseminatedFemale Spermatheca    288.4 Adult Ovary    326 Adult Testis    22.1 Adult Male Accessory Gland    128 Adult Carcass    83.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    581.275 Larval Midgut    180.3 Larval Hindgut    213.6 Larval Malpighian Tubules    201.5 Larval Fat Body    89.9 Larval Salivary Gland    107 Larval Trachea    200.9 Larval Carcass    142.325 Adult Head    300.1 Adult Eye    547.2 Adult Brain    1274.3 Adult Thoracic-Abdominal Ganglion    1237.3 Adult Crop    244.1 Adult Midgut    152.5 Adult Hindgut    260.6 Adult Malpighian Tubules    169.8 Adult Fat Body    69.3 Adult Salivary Gland    242.9 Adult Heart    141.45 Adult VirginFemale Spermatheca    302 Adult InseminatedFemale Spermatheca    288.4 Adult Ovary    326 Adult Testis    22.1 Adult Male Accessory Gland    128 Adult Carcass    83.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  581.275 Larval Midgut    180.3 Larval Hindgut    213.6 Larval Malpighian Tubules    201.5 Larval Fat Body    89.9 Larval Salivary Gland    107 Larval Trachea    200.9 Larval Carcass    142.325 Adult Head    300.1 Adult Eye  547.2 Adult Brain  (1274.3) Adult Thoracic-Abdominal Ganglion  (1237.3) Adult Crop    244.1 Adult Midgut    152.5 Adult Hindgut    260.6 Adult Malpighian Tubules    169.8 Adult Fat Body    69.3 Adult Salivary Gland    242.9 Adult Heart    141.45 Adult VirginFemale Spermatheca    302 Adult InseminatedFemale Spermatheca    288.4 Adult Ovary    326 Adult Testis    22.1 Adult Male Accessory Gland    128 Adult Carcass    83.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    581.275 Larval Midgut    180.3 Larval Hindgut    213.6 Larval Malpighian Tubules    201.5 Larval Fat Body    89.9 Larval Salivary Gland    107 Larval Trachea    200.9 Larval Carcass    142.325 Adult Head    300.1 Adult Eye    547.2 Adult Brain  1274.3 Adult Thoracic-Abdominal Ganglion  1237.3 Adult Crop    244.1 Adult Midgut    152.5 Adult Hindgut    260.6 Adult Malpighian Tubules    169.8 Adult Fat Body    69.3 Adult Salivary Gland    242.9 Adult Heart    141.45 Adult VirginFemale Spermatheca    302 Adult InseminatedFemale Spermatheca    288.4 Adult Ovary    326 Adult Testis    22.1 Adult Male Accessory Gland    128 Adult Carcass    83.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    581.275 Larval Midgut    180.3 Larval Hindgut    213.6 Larval Malpighian Tubules    201.5 Larval Fat Body    89.9 Larval Salivary Gland    107 Larval Trachea    200.9 Larval Carcass    142.325 Adult Head    300.1 Adult Eye    547.2 Adult Brain    1274.3 Adult Thoracic-Abdominal Ganglion    1237.3 Adult Crop    244.1 Adult Midgut    152.5 Adult Hindgut    260.6 Adult Malpighian Tubules    169.8 Adult Fat Body    69.3 Adult Salivary Gland    242.9 Adult Heart    141.45 Adult VirginFemale Spermatheca    302 Adult InseminatedFemale Spermatheca    288.4 Adult Ovary    326 Adult Testis    22.1 Adult Male Accessory Gland    128 Adult Carcass    83.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    300.1   NA  Eye    547.2   NA  Brain    1274.3   581.275  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1237.3   NA  Crop    244.1   180.3  Midgut    152.5   213.6  Hindgut    260.6   201.5  Malpighian Tubules    169.8   89.9  Fat Body    69.3   107  Salivary Gland    242.9   NA  Heart    141.45   200.9  Trachea    NA   NA  VirginFemale Spermatheca    302   NA  InseminatedFemale Spermatheca    288.4   NA  Ovary    326   NA  Testis    22.1   NA  Male Accessory Gland    128   142.325  Carcass    83.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    88 central nervous system, pupae P8    227 head, virgin 1-day female    137 head, virgin 4-day female    124 head, virgin 20-day female    47 head, mated 1-day female    133 head, mated 4-day female    111 head, mated 20-day female    50 head, mated 1-day male    192 head, mated 4-day male    209 head, mated 20-day male    150 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    7 fat body, pupae P8    40 carcass, larvae L3 wandering    17 carcass, 1-day adult    14 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    21 ovary, mated 4-day female    29 testis, mated 4-day male    1 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3  (88) central nervous system, pupae P8  (227) head, virgin 1-day female  (137) head, virgin 4-day female  (124) head, virgin 20-day female  (47) head, mated 1-day female  (133) head, mated 4-day female  (111) head, mated 20-day female  (50) head, mated 1-day male  (192) head, mated 4-day male  (209) head, mated 20-day male  (150) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    7 fat body, pupae P8  (40) carcass, larvae L3 wandering    17 carcass, 1-day adult    14 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    21 ovary, mated 4-day female  (29) testis, mated 4-day male    1 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    88 central nervous system, pupae P8  (227) head, virgin 1-day female  (137) head, virgin 4-day female  (124) head, virgin 20-day female    47 head, mated 1-day female  (133) head, mated 4-day female  (111) head, mated 20-day female    50 head, mated 1-day male  (192) head, mated 4-day male  (209) head, mated 20-day male  (150) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    7 fat body, pupae P8    40 carcass, larvae L3 wandering    17 carcass, 1-day adult    14 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    21 ovary, mated 4-day female    29 testis, mated 4-day male    1 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    88 central nervous system, pupae P8    227 head, virgin 1-day female    137 head, virgin 4-day female    124 head, virgin 20-day female    47 head, mated 1-day female    133 head, mated 4-day female    111 head, mated 20-day female    50 head, mated 1-day male    192 head, mated 4-day male    209 head, mated 20-day male    150 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    7 fat body, pupae P8    40 carcass, larvae L3 wandering    17 carcass, 1-day adult    14 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    21 ovary, mated 4-day female    29 testis, mated 4-day male    1 accessory gland, mated 4-day male    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    88 central nervous system, pupae P8    227 head, virgin 1-day female    137 head, virgin 4-day female    124 head, virgin 20-day female    47 head, mated 1-day female    133 head, mated 4-day female    111 head, mated 20-day female    50 head, mated 1-day male    192 head, mated 4-day male    209 head, mated 20-day male    150 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    7 fat body, pupae P8    40 carcass, larvae L3 wandering    17 carcass, 1-day adult    14 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    21 ovary, mated 4-day female    29 testis, mated 4-day male    1 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3  (88) central nervous system, pupae P8  (227) head, virgin 1-day female  (137) head, virgin 4-day female  (124) head, virgin 20-day female  (47) head, mated 1-day female  (133) head, mated 4-day female  (111) head, mated 20-day female  (50) head, mated 1-day male  (192) head, mated 4-day male  (209) head, mated 20-day male  (150) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    7 fat body, pupae P8  (40) carcass, larvae L3 wandering    17 carcass, 1-day adult    14 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    21 ovary, mated 4-day female  29 testis, mated 4-day male    1 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    88 central nervous system, pupae P8  (227) head, virgin 1-day female  137 head, virgin 4-day female  124 head, virgin 20-day female    47 head, mated 1-day female  133 head, mated 4-day female  111 head, mated 20-day female    50 head, mated 1-day male  (192) head, mated 4-day male  (209) head, mated 20-day male  (150) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    7 fat body, pupae P8    40 carcass, larvae L3 wandering    17 carcass, 1-day adult    14 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    21 ovary, mated 4-day female    29 testis, mated 4-day male    1 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    88 central nervous system, pupae P8    227 head, virgin 1-day female    137 head, virgin 4-day female    124 head, virgin 20-day female    47 head, mated 1-day female    133 head, mated 4-day female    111 head, mated 20-day female    50 head, mated 1-day male    192 head, mated 4-day male    209 head, mated 20-day male    150 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    7 fat body, pupae P8    40 carcass, larvae L3 wandering    17 carcass, 1-day adult    14 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    21 ovary, mated 4-day female    29 testis, mated 4-day male    1 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during early larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    9 embryo 04-06hr    7 embryo 06-08hr    7 embryo 08-10hr    7 embryo 10-12hr    7 embryo 12-14hr    10 embryo 14-16hr    15 embryo 16-18hr    21 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    24 larva L1    22 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    15 white prepupae 12hr    16 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    22 pupae 4d postWPP    18 adult male 01day    16 adult male 05day    16 adult male 30day    17 adult female 01day    20 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    9 embryo 04-06hr    7 embryo 06-08hr    7 embryo 08-10hr    7 embryo 10-12hr    7 embryo 12-14hr    10 embryo 14-16hr    15 embryo 16-18hr    21 embryo 18-20hr  26 embryo 20-22hr    22 embryo 22-24hr    24 larva L1    22 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    15 white prepupae 12hr    16 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    22 pupae 4d postWPP    18 adult male 01day    16 adult male 05day    16 adult male 30day    17 adult female 01day    20 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    9 embryo 04-06hr    7 embryo 06-08hr    7 embryo 08-10hr    7 embryo 10-12hr    7 embryo 12-14hr    10 embryo 14-16hr    15 embryo 16-18hr    21 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    24 larva L1    22 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    15 white prepupae 12hr    16 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    22 pupae 4d postWPP    18 adult male 01day    16 adult male 05day    16 adult male 30day    17 adult female 01day    20 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    9 embryo 04-06hr    7 embryo 06-08hr    7 embryo 08-10hr    7 embryo 10-12hr    7 embryo 12-14hr    10 embryo 14-16hr    15 embryo 16-18hr    21 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    24 larva L1    22 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    15 white prepupae 12hr    16 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    22 pupae 4d postWPP    18 adult male 01day    16 adult male 05day    16 adult male 30day    17 adult female 01day    20 adult female 05day    19 adult female 30day    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    9 embryo 04-06hr    7 embryo 06-08hr    7 embryo 08-10hr    7 embryo 10-12hr    7 embryo 12-14hr    10 embryo 14-16hr    15 embryo 16-18hr    21 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    24 larva L1    22 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    15 white prepupae 12hr    16 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    22 pupae 4d postWPP    18 adult male 01day    16 adult male 05day    16 adult male 30day    17 adult female 01day    20 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    9 embryo 04-06hr    7 embryo 06-08hr    7 embryo 08-10hr    7 embryo 10-12hr    7 embryo 12-14hr    10 embryo 14-16hr    15 embryo 16-18hr    21 embryo 18-20hr  26 embryo 20-22hr    22 embryo 22-24hr    24 larva L1    22 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    15 white prepupae 12hr    16 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    22 pupae 4d postWPP    18 adult male 01day    16 adult male 05day    16 adult male 30day    17 adult female 01day    20 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    9 embryo 04-06hr    7 embryo 06-08hr    7 embryo 08-10hr    7 embryo 10-12hr    7 embryo 12-14hr    10 embryo 14-16hr    15 embryo 16-18hr    21 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    24 larva L1    22 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    15 white prepupae 12hr    16 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    22 pupae 4d postWPP    18 adult male 01day    16 adult male 05day    16 adult male 30day    17 adult female 01day    20 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    23 embryo 02-04hr    9 embryo 04-06hr    7 embryo 06-08hr    7 embryo 08-10hr    7 embryo 10-12hr    7 embryo 12-14hr    10 embryo 14-16hr    15 embryo 16-18hr    21 embryo 18-20hr    26 embryo 20-22hr    22 embryo 22-24hr    24 larva L1    22 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    15 white prepupae 12hr    16 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    22 pupae 4d postWPP    18 adult male 01day    16 adult male 05day    16 adult male 30day    17 adult female 01day    20 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    10 embryonic 1182-4H    12 embryonic GM2    14 embryonic Kc167    13 embryonic S1    18 embryonic S3    12 leg disc CME L1    10 wing disc CME-W2    8 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    41 tumorous blood cells mbn2    11 ovary fGS/OSS    26 ovary OSC    37 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    10 embryonic 1182-4H    12 embryonic GM2    14 embryonic Kc167    13 embryonic S1    18 embryonic S3    12 leg disc CME L1    10 wing disc CME-W2    8 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2  (41) tumorous blood cells mbn2    11 ovary fGS/OSS  26 ovary OSC  (37) ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    10 embryonic 1182-4H    12 embryonic GM2    14 embryonic Kc167    13 embryonic S1    18 embryonic S3    12 leg disc CME L1    10 wing disc CME-W2    8 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    41 tumorous blood cells mbn2    11 ovary fGS/OSS    26 ovary OSC    37 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    10 embryonic 1182-4H    12 embryonic GM2    14 embryonic Kc167    13 embryonic S1    18 embryonic S3    12 leg disc CME L1    10 wing disc CME-W2    8 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    41 tumorous blood cells mbn2    11 ovary fGS/OSS    26 ovary OSC    37 ovary OSS    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    10 embryonic 1182-4H    12 embryonic GM2    14 embryonic Kc167    13 embryonic S1    18 embryonic S3    12 leg disc CME L1    10 wing disc CME-W2    8 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    41 tumorous blood cells mbn2    11 ovary fGS/OSS    26 ovary OSC    37 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    10 embryonic 1182-4H    12 embryonic GM2    14 embryonic Kc167    13 embryonic S1    18 embryonic S3    12 leg disc CME L1    10 wing disc CME-W2    8 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2  (41) tumorous blood cells mbn2    11 ovary fGS/OSS  26 ovary OSC  (37) ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    10 embryonic 1182-4H    12 embryonic GM2    14 embryonic Kc167    13 embryonic S1    18 embryonic S3    12 leg disc CME L1    10 wing disc CME-W2    8 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    41 tumorous blood cells mbn2    11 ovary fGS/OSS    26 ovary OSC    37 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    10 embryonic 1182-4H    12 embryonic GM2    14 embryonic Kc167    13 embryonic S1    18 embryonic S3    12 leg disc CME L1    10 wing disc CME-W2    8 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    8 wing disc ML-DmD32    12 haltere disc ML-DmD17-c3    15 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    41 tumorous blood cells mbn2    11 ovary fGS/OSS    26 ovary OSC    37 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    22 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    32 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    41 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    43 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    29 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    27 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  26 cold shock, 4-day adult    22 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult  (32) Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult  (41) Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult  (43) Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (29) Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (29) Paraquat 10 mM 48 hrs, 4-day adult  (27) Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    22 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    32 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    41 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    43 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    29 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    27 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    22 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    32 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    41 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    43 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    29 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    27 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    22 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    32 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    41 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    43 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    29 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    27 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  26 cold shock, 4-day adult    22 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult  32 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult  (41) Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult  (43) Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  29 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  29 Paraquat 10 mM 48 hrs, 4-day adult  27 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    22 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    32 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    41 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    43 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    29 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    27 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    22 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    32 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    41 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    43 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    29 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    27 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_10 mE1_20_mRNA_expression_cluster_15
External Data & Images
Linkouts	BDGP expression data • RE01835 RE09582 RH26066 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013759 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6703-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for CASK Stages(s) 1-3 Stages(s) 4-6 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable CASKEY07081 viable, with Scer\GAL4pnr-MD237 CASKGD10585 Sterility Allele fertile CASKEY07081 CASKP18 Other Phenotypes Allele courtship behavior defective CASKX-307 courtship behavior defective | male CASKP18 courtship behavior defective | male (with CASKX-307) CASKX-313 courtship behavior defective | male (with CASKX-313) CASKX-307 flight defective (with CASKX-307) CASKX-313 flight defective (with CASKX-313) CASKX-307 locomotor behavior defective CASKX-307 CASKX-313 locomotor behavior defective, with CASKP18, Scer\GAL4C164 CASKScer\UAS.cBa locomotor behavior defective, with CASKScer\UAS.cBa, Scer\GAL4C164 CASKP18 locomotor behavior defective (with CASKP18) CASKX-307 CASKX-313 locomotor behavior defective (with CASKX-307) CASKP18 CASKX-313 locomotor behavior defective (with CASKX-313) CASKP18 CASKX-307 locomotor behavior defective (with Df(3R)Exel6187) CASKP18 locomotor behavior defective | dominant CASKP18 locomotor behavior defective | recessive CASKP18 neurophysiology defective (with CASKX-307) CASKX-313 neurophysiology defective (with CASKX-313) CASKX-307 visible, with Scer\GAL4pnr-MD237 CASKGD10585 visual behavior defective (with CASKX-307) CASKX-313 visual behavior defective (with CASKX-313) CASKX-307 Phenotype manifest in Allele chaeta, with Scer\GAL4pnr-MD237 CASKGD10585 trichogen cell, with Scer\GAL4pnr-MD237 CASKGD10585
Classical Alleles ( 14 )
For All Classical Alleles Show
Allele of CASK Class Mutagen Stocks Known lesion CASKEY07081 P-element activity 1 -- CASKf00990 piggyBac transposase 1 -- CASKf07134 piggyBac transposase 1 -- CASKMB06983 1 -- CASKMI01748 1 -- CASKX-307 X ray 1 Yes CASKX-313 X ray 1 Yes CASKd00541 0 -- CASKd05257 0 -- CASKd11720 0 -- CASKP18 hypomorphic allele - molecular evidence Delta2-3 0 Yes CASKP29 hypomorphic allele - molecular evidence Delta2-3 0 Yes CASKP46 hypomorphic allele - molecular evidence Delta2-3 0 Yes CASKT119 hypomorphic allele - molecular evidence Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CASK Class Mutagen Stocks Known lesion CASKGD10585 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CASKGL00212 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CASKHMS00644 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CASKJF02710 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CASKKK109311 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CASKScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes CASKScer\UAS.cZa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)cakiX-313 (Martin and Ollo, 1996) Df(3R)Exel6187 (Ryder, 2004.4.26) Df(3R)X-307 (Grillo et al., 2012)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{TRiP.GL00212} NA P{TRiP.HMS00644} NA P{UAS-caki.Z} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}CASKEY07081 NA P{XP}Cakid00753 NA P{XP}CASKd00541 NA P{XP}CASKd05257 NA P{XP}CASKd11720 NA miscellaneous insertions Mi{MIC}CASKMI01748 NA PBac{WH}CASKf00990 NA PBac{WH}CASKf07134 NA insertion of enhancer trap binary system Mi{ET1}CASKMB06983 No
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
calmodulin-dependent protein kinase activity inferred from direct assay AND inferred from sequence or structural similarity (Martin and Ollo, 1996)
Biological Process
CV term Evidence References
adult locomotory behavior inferred from mutant phenotype (Slawson et al., 2011) adult walking behavior inferred from mutant phenotype (Martin and Ollo, 1996) cell adhesion inferred from mutant phenotype (Kiger et al., 2003) lateral inhibition inferred from mutant phenotype (Mummery-Widmer et al., 2009) male courtship behavior inferred from mutant phenotype (Slawson et al., 2011) regulation of cell shape inferred from mutant phenotype (Kiger et al., 2003) regulation of neurotransmitter secretion inferred from direct assay (Zordan et al., 2005)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290 (FlyBase Curators et al., 2004-) protein serine/threonine kinase activity non-traceable author statement (Morrison et al., 2000)
Biological Process
CV term Evidence References
establishment or maintenance of cell polarity non-traceable author statement (Humbert et al., 2003) neurotransmitter secretion non-traceable author statement (Lloyd et al., 2000) protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290 (FlyBase Curators et al., 2004-) non-traceable author statement (Morrison et al., 2000) synaptic vesicle docking involved in exocytosis non-traceable author statement (Lloyd et al., 2000) synaptic vesicle targeting non-traceable author statement (Lloyd et al., 2000)
Cellular Component
CV term Evidence References
calcium- and calmodulin-dependent protein kinase complex inferred from sequence or structural similarity (Martin and Ollo, 1996)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) gene_with_stop_codon_read_through (Jungreis et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
CASK - CG33095 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CASK - veli anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CASK allele Gene References unspecified H (Mueller et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67530 DroID - A comprehensive database of gene and protein interactions. • FBgn0013759 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42567 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q24210
Orthologs
OrthoDB Orthologs (112) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6SBFH9 Dipteran inclusive ortholog search EOG6QZ7TX Insect inclusive ortholog search EOG6BRVG1 Arthropod inclusive ortholog search EOG65X6BD Metazoa inclusive ortholog search EOG6W45F5
Orthologs in Drosophila Species (EOG6SBFH9)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CASK     Drosophila simulans   Dsim\GD18503 Y   Drosophila sechellia   Dsec\GM23693 Y   Drosophila erecta   Dere\GG22470 Y   Drosophila yakuba   Dyak\GE24086 Y   Drosophila ananassae   Dana\GF16967 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19796 Y   Drosophila persimilis   Dper\GL23741 Y   Drosophila willistoni   Dwil\GK13019 Y   Drosophila virilis   Dvir\GJ14160 Y   Drosophila mojavensis   Dmoj\GI22862 Y   Drosophila grimshawi   Dgri\GH18714 Y
Orthologs in non-Drosophila Dipterans (EOG6QZ7TX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL003914   Anopheles gambiae Malaria mosquito  Agam\AGAP001683   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001163
Orthologs in non-Dipteran Insects (EOG6BRVG1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB53996   Apis mellifera Western honey bee  Amel\GB53989   Apis mellifera Western honey bee  Amel\GB53990   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG011836   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH22009   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH22002   Atta cephalotes Leafcutter ant  Acep\ACEP22476   Atta cephalotes Leafcutter ant  Acep\ACEP22474   Atta cephalotes Leafcutter ant  Acep\ACEP22480   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15822   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15825   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21751   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18607   Linepithema humile Argentine ant  Lhum\LH22792   Linepithema humile Argentine ant  Lhum\LH22799   Linepithema humile Argentine ant  Lhum\LH22798   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20368   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20357   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20363   Solenopsis invicta Red fire ant  Sinv\SINV23171   Solenopsis invicta Red fire ant  Sinv\SINV23207   Solenopsis invicta Red fire ant  Sinv\SINV23243   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004522   Bombyx mori Silkmoth  Bmor\BGIBMGA006299   Bombyx mori Silkmoth  Bmor\BGIBMGA006298   Tribolium castaneum Red flour beetle  Tcas\TC002673
Orthologs in non-Insect Arthropods (EOG65X6BD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COII   Ixodes scapularis Deer tick  Isca\ISCW022928   Ixodes scapularis Deer tick  Isca\ISCW022930
Orthologs in non-Arthropod Metazoa (EOG6W45F5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\tag-117 Y Caenorhabditis elegans Nematode  Cele\Y55B1BR.4 Y Caenorhabditis elegans Nematode  Cele\lin-2 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003310gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016842gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004864gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003457gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001995gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016934gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024853gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028366gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023432gn Y Danio rerio Zebrafish  Drer\mpp2a Y Danio rerio Zebrafish  Drer\ENSDARG00000025168 Y Danio rerio Zebrafish  Drer\ENSDARG00000035103 Y Danio rerio Zebrafish  Drer\mpp7 Y Danio rerio Zebrafish  Drer\ENSDARG00000062667 Y Danio rerio Zebrafish  Drer\OTTDARG00000031520 Y Danio rerio Zebrafish  Drer\mpp1 Y Danio rerio Zebrafish  Drer\mpp6b Y Danio rerio Zebrafish  Drer\mpp5b Y Danio rerio Zebrafish  Drer\mpp4a Y Danio rerio Zebrafish  Drer\mpp2b Y Danio rerio Zebrafish  Drer\ENSDARG00000015184 Y Danio rerio Zebrafish  Drer\mpp6a Y Danio rerio Zebrafish  Drer\cask Y Danio rerio Zebrafish  Drer\ENSDARG00000041883 Y Danio rerio Zebrafish  Drer\mpp5a Y Danio rerio Zebrafish  Drer\ENSDARG00000076872 Y Xenopus tropicalis Western clawed frog  Xtro\mpp1 Y Xenopus tropicalis Western clawed frog  Xtro\mpp6 Y Xenopus tropicalis Western clawed frog  Xtro\cask Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031296 Y Xenopus tropicalis Western clawed frog  Xtro\mpp2 Y Xenopus tropicalis Western clawed frog  Xtro\mpp7 Y Xenopus tropicalis Western clawed frog  Xtro\mpp5 Y Gallus gallus Domestic chicken  Ggal\MPP1 Y Gallus gallus Domestic chicken  Ggal\MPP3 Y Gallus gallus Domestic chicken  Ggal\MPP4 Y Gallus gallus Domestic chicken  Ggal\MPP6 Y Gallus gallus Domestic chicken  Ggal\CASK Y Gallus gallus Domestic chicken  Ggal\MPP7 Y Gallus gallus Domestic chicken  Ggal\MPP5 Y Gallus gallus Domestic chicken  Ggal\MPP2 Y Mus musculus House mouse  Mmus\Mpp2 Y Mus musculus House mouse  Mmus\Mpp6 Y Mus musculus House mouse  Mmus\Mpp7 Y Mus musculus House mouse  Mmus\Mpp4 Y Mus musculus House mouse  Mmus\Mpp1 Y Mus musculus House mouse  Mmus\Mpp5 Y Mus musculus House mouse  Mmus\Mpp3 Y Mus musculus House mouse  Mmus\ENSMUSG00000031012 Y Rattus norvegicus Norway rat  Rnor\Cask Y Rattus norvegicus Norway rat  Rnor\Mpp3 Y Rattus norvegicus Norway rat  Rnor\Mpp5 Y Rattus norvegicus Norway rat  Rnor\Mpp6 Y Rattus norvegicus Norway rat  Rnor\Mpp4 Y Rattus norvegicus Norway rat  Rnor\Mpp2 Y Rattus norvegicus Norway rat  Rnor\Mpp7 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000038657 Y Homo sapiens Human  Hsap\MPP5 Y Homo sapiens Human  Hsap\MPP3 Y Homo sapiens Human  Hsap\MPP7 Y Homo sapiens Human  Hsap\MPP6 Y Homo sapiens Human  Hsap\MPP1 Y Homo sapiens Human  Hsap\ENSG00000108852 Y Homo sapiens Human  Hsap\MPP4 Y Homo sapiens Human  Hsap\ENSG00000147044 Y
Human Orthologs (8)
Gene OMIM HGNC Hsap\MPP5     Hsap\MPP3     Hsap\MPP7     Hsap\MPP6     Hsap\MPP1     Hsap\ENSG00000108852     Hsap\MPP4     Hsap\ENSG00000147044
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD18503 Drosophila sechellia Dsec\GM23693 Drosophila erecta Dere\GG22470 Drosophila yakuba Dyak\GE24086 Drosophila ananassae Dana\GF16967 Drosophila pseudoobscura pseudoobscura Dpse\GA19796 Drosophila persimilis Dper\GL23741 Drosophila willistoni Dwil\GK13019 Drosophila virilis Dvir\GJ14160 Drosophila mojavensis Dmoj\GI22862 Drosophila grimshawi Dgri\GH18714
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	6783 CASKX-307/TM3, Sb1 27556 y1 v1; P{TRiP.JF02710}attP2 16787 y1 w67c23; P{EPgy2}CASKEY07081 35309 y1 sc* v1; P{TRiP.GL00212}attP2 32857 y1 sc* v1; P{TRiP.HMS00644}attP2 26063 w1118; Mi{ET1}CASKMB06983/TM6C, Sb1 6784 w*; Df(3R)cakiX-313, CASKX-313/TM6B, Tb+
Harvard	- PBac{WH}CASKf00990 - PBac{WH}CASKf07134
VDRC	v104793 P{KK109311}VIE-260B v34184 w1118; P{GD10585}v34184/TM3 v34185 w1118; P{GD10585}v34185/CyO
	More stocks available...
Genomic Clones ( 1 )
	BACR42I20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 129 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE01835 RE09582 RH26066
Other clones	FI02017 FI03206 MIP07992
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM15368 GM26591 HL01064 HL01359 LD19143 LD46240 RE03045 RE03083 RE03767 RE06904 RE12239 RE39382 RE42388 RE48024 RH10570 RH46561 SD05926
Other clones	1494 8716 28740 54147 60985 64129 75570 78667 79711 83868 99062 103231 107904 108622 121039 156545 158680 172630 174402 175969 179700 283422 293838 CK01.7B.A12 CK01.30B.F10 CK01.60D.G4 CK01.74D.G4 CK01.81A.D5 CK01.84C.A8 CK01.85A.D7 CK01.102A.E11 CK01.103B.D4 CK01.103B.F4 CK01.105D.C3 CK01.118B.F8 CK01.120B.E10 CK01.123B.B12 CK01.123C.H11 CK01.133B.E11 Dmel_neb_012277_0822_1910 EC053546 EC053551 EC054031 EC054039 EC054528 EC055349 EC055930 EC056205 EC056452 EC056560 EC253395 EC254149 EC257198 EK048944 EK058758 EK067125 EK070707 EK085920 EK088410 EK090056 EK093932 EK093938 EK102018 EK107332 EK122251 EK136825 EK138904 EK163661 EK179320 EK185759 EK190060 EK206330 EK207426 EK209859 EK216002 EK218346 EK234333 EK242914 EK244746 EK260033 EK261851 EK264807 EK272653 EK275654 EK278804 EK280955 EK308928 EN11143 EP07146 EP14015 EP19209 FGC001E11 FGH105A12 FGM232H12 RP004074890 RP004131409 RP004154337 RP004162264 RP004169917 RP004239154 RP004258890 RP004268184 RP004452155 RP004457633 RP004501420 RP004530045
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013759 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42567
Antibody Information
	polyclonal (Chen and Featherstone, 2011, Martin and Ollo, 1996)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: CASK Caki (Griffith et al., 2010.9.7)
Source for database merge of	Source for merge of: Caki Cask (Verstreken, 2005.7.11) Source for merge of: Caki CG13413 (FlyBase Genome Annotators, 2002-2003) Source for merge of: Caki cmg (Dimitratos et al., 1997)
Additional comments	mir-999 is located within CASK. (Stark et al., 2007) Annotations CG6703, CG13412 and CG13413 merged as CG6703 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	Caki enhances the eag potassium current by a phosphorylation-dependent mechanism. (Marble et al., 2005) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cells become round and detached. Kc167 cells are unaffected. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Cloning and characterisation of Caki. (Dimitratos et al., 1997) The Caki protein sequence is conserved in the catalytic and calmodulin-binding domains with other type II CaM kinases. In cultured cells Caki has the ability to stimulate the transcriptional activity of the Rous sarcoma virus long terminal repeat (RSV LTR). (Martin and Ollo, 1996) Mutant flies, in which an encoding part of the gene is removed, display a substantial reduction in walking speed. (Martin and Ollo, 1996) Expressed in nurse cells. (Martin et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079717 NM_169970 NM_169971 NM_001104392 NM_001104393 NM_001170210 NM_001275882 RefSeq (Proteins) • NP_524441 NP_732661 NP_732662 NP_001097862 NP_001097863 NP_001163681 NP_001262811 DDBJ / EMBL / GenBank • AAC80169 AAF55920 AAF55921 AAF55922 AAM11269 AAO39536 AAO39554 ABW08723 ABW08724 ACZ94977 AGB96191 AY094916 BI162546 BI168732 BI586211 BT003532 BT003550 CAA63940 CL527975 CZ483474 CZ483475 CZ489912 CZ489913 U53190 X94264 Entrez Gene - A searchable database of RefSeq genes. • 42567 UniProtKB/Swiss-Prot • Q24210 UniProtKB/TrEMBL • A8JR71 A8JR72 E1JIS7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Src homology-3 domain (IPR001452) PDZ domain (IPR001478) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) L27 (IPR004172) Guanylate kinase (IPR008144) Guanylate kinase/L-type calcium channel (IPR008145) Protein kinase-like domain (IPR011009) Variant SH3 (IPR011511) L27, C-terminal (IPR014775) Guanylate kinase, conserved site (IPR020590) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE01835 RE09582 RH26066
Linkouts
	BioGRID - A database of protein and genetic interactions • 67530 DroID - A comprehensive database of gene and protein interactions. • FBgn0013759 DRSC - Results from RNAi screens. • FBgn0013759 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013759 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6703-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013759 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42567 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/caki1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42567 modMine - Data generated by the modENCODE project. • FBgn0013759 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q24210
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	caki (Mehren et al., 2004, Megighian et al., 2002, Martin, 1995.12.14, Martin and Ollo, 1996, Southall and Brand, 2009, Peng et al., 2007, Zordan et al., 2005) CAKI (Bellen, 2000.12.25, Morrison et al., 2000, Lloyd et al., 2000, Sun et al., 2009, Zordan et al., 2005) Caki (Guan et al., 2011, Jungreis et al., 2011, Wang et al., 2004, Wu et al., 2007, Bakal et al., 2008, Adryan et al., 2007, Stark et al., 2007, Smibert et al., 2012) CamGUK (Ponting et al., 1997) Camguk (Marble et al., 2005) CamguK (Guan et al., 2011) CAMGUK (Zordan et al., 2005) camguk (Zordan et al., 2005) CASK (Lloyd et al., 2000, Tian et al., 2013, Guan et al., 2011, Griffith et al., 2010.9.7, Slawson et al., 2011) Cask   CG6703 (Morrison et al., 2000, Verstreken and Bellen, 2000.9.4, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2010.12.1, Ni et al., 2011.7.19) CG13412 (Morrison et al., 2000, Verstreken and Bellen, 2000.9.4, ) CG13413 (Whitfield, 2000.4.4, ) Cmg (Lu et al., 2003) cmg (Lopes et al., 2001, Whitfield, 2000.7.14, Whitfield, 2000.4.4, Dimitratos, 1996.3.31, Dimitratos et al., 1999, Dimitratos et al., 1997, Dimitratos et al., 1997, Dimitratos et al., 1997, Dimitratos et al., 1996, Bachmann et al., 2010) CMG (Marble et al., 2005, Sun et al., 2009) CMG/CASK (Morrison et al., 2000) dCASK (Hodge et al., 2006) DLin-2 (Bachmann et al., 2004) RE09582p (Chen et al., 2007)
Name Synonym	Calcium/calmodulin-dependent protein kinase (Guan et al., 2011, Martin et al., 1994) calmodulin-dependent kinase (Harris and Lim, 2001) Camguk (Doerks et al., 2000, Colbran and Brown, 2004, Humbert et al., 2003, Caruana, 2002, de Lorenzo et al., 2003, Lee et al., 2001, de Lorenzo et al., 2001, Lu et al., 2003, Dimitratos, 1996.3.31, Lee et al., 2002, Bachmann et al., 2010) camguk (MacMullin et al., 2001, Mehren et al., 2004, Dimitratos et al., 1997, Dimitratos et al., 1996) Camguk/CASK adaptor protein (Marble et al., 2005) CASK ortholog
Secondary FlyBase IDs
	FBgn0015923 FBgn0038904 FBgn0043003
References ( 91 )
Generate a list of	All references relating to this gene ( 91 )
List References by type	Research paper  ( 32 ) Supplementary material  ( 11 ) Review  ( 10 ) Personal communication to FlyBase  ( 19 ) Abstract  ( 8 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Website  ( 1 ) Curated genome annotation  ( 3 ) Letter  ( 1 ) Protein sequence record  ( 1 )
Recent research papers ( 5 )
	Tian et al., 2013, Neuron 77(2): 311--322 Neurexin Regulates Visual Function via Mediating Retinoid Transport to Promote Rhodopsin Maturation. [FBrf0220614] Grillo et al., 2012, Sci. Rep. 2: 762 Conserved and divergent elements in Torso RTK activation in Drosophila development. [FBrf0219757] Chen and Featherstone, 2011, Mol. Cell. Neurosci. 48(2): 171--182 Pre and postsynaptic roles for Drosophila CASK. [FBrf0214784] Guan et al., 2011, Learn. Mem. 18(4): 191--206 Altered gene regulation and synaptic morphology in Drosophila learning and memory mutants. [FBrf0213277] Slawson et al., 2011, Genetics 187(1): 171--184 Central Regulation of Locomotor Behavior of Drosophila melanogaster Depends on a CASK Isoform Containing CaMK-Like and L27 Domains. [FBrf0212754] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...