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Gene Dmel\Cdk5

General Information
Symbol	Dmel\Cdk5	Species	D. melanogaster
Name	Cyclin-dependent kinase 5	Annotation symbol	CG8203
Feature type	protein_coding_gene	FlyBase ID	FBgn0013762
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	52A13-52A13	Sequence location	2R:11,457,033..11,458,922 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Cyclin-dependent kinase 5 is referred to in FlyBase by the symbol Dmel\Cdk5 (CG8203, FBgn0013762). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: cyclin-dependent protein serine/threonine kinase activity; protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: motor neuron axon guidance; determination of adult lifespan; adult locomotory behavior; protein phosphorylation; flight behavior; melanotic encapsulation of foreign target; axonogenesis; cell cycle; intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; regulation of synaptic growth at neuromuscular junction. 10 alleles are reported. The phenotype of these alleles is annotated with: presumptive embryonic/larval nervous system. It has one annotated transcript and one annotated polypeptide. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1637085_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Cdk5. Gene sequence location is 2R:11457033..11458922.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 52A13-52A13   Limits computationally determined from genome sequence between P{lacW}Vha36k07207 and P{lacW}l(2)k05713k05713
Experimentally Determined Cytological Location
Cytogenetic map Notes References 52A-52A     (Lehner, 1996.7.23) 52A14-52C9   (determined by in situ hybridisation)   (Hellmich et al., 1994)
Experimentally Determined Recombination Data
Location	2- (Lehner, 1996.7.23)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Cdk5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Cdk5-RA FBtr0087350  NM_057732   1604   294
Additional Transcript Data & Comments
Reported size (kB)	1.6 (northern blot) (Sauer et al., 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Cdk5-PA   FBpp0086483   33.2   294   7.82     NP_477080   AAF58119
Additional Polypeptide Data & Comments
Reported size (kDa)	294 (aa) (Sauer et al., 1996)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA816484   AE013599 AAF58119   AC006242   AC008369   AW941483   AY061049 AAL28597   BI608064   BI610200   BI610668   BI621709   BI623830   BI631040   BI640637   BP559232   CO318951   CO319252   EC052536   EC059709   EC066982   EC078464   EC238869   EC259454   EL874935   U21552 AAA63754   X99511 CAA67861
UniProtKB/Swiss-Prot	P48609
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval central nervous system   (Sauer et al., 1996) presumptive embryonic/larval nervous system   (Sauer et al., 1996) embryonic stage | early   organism | ubiquitous   (Sauer et al., 1996) embryonic stage -- adult stage     (Sauer et al., 1996)
Additional Descriptive Data
	Cdk5 transcripts are detected at all stages of development on northern blots. They are found to be ubiquitous in early embryos by in situ hybridization. They are also present at a low level throughout the embryo at later stages of embryogenesis. Stronger signals are observed in the CNS of late embryos. (Sauer et al., 1996)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Sauer et al., 1996)
Additional Descriptive Data
	Constant levels of Cdk5 protein are observed through embryogenesis by immunoblotting. (Sauer et al., 1996)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cyclin-dependent protein kinase 5 holoenzyme complex inferred from physical interaction with Cdk5α (Connell-Crowley et al., 2000)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    329.2 Larval Midgut    211.6 Larval Hindgut    175.1 Larval Malpighian Tubules    244.5 Larval Fat Body    286.2 Larval Salivary Gland    216.6 Larval Trachea    202.975 Larval Carcass    143.8 Adult Head    223.2 Adult Eye    241.975 Adult Brain    237.5 Adult Thoracic-Abdominal Ganglion    251.1 Adult Crop    187.8 Adult Midgut    207.6 Adult Hindgut    167.5 Adult Malpighian Tubules    165.8 Adult Fat Body    213.7 Adult Salivary Gland    191.5 Adult Heart    179.25 Adult VirginFemale Spermatheca    242.9 Adult InseminatedFemale Spermatheca    254.8 Adult Ovary    352.2 Adult Testis    29 Adult Male Accessory Gland    145.3 Adult Carcass    149.3 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    329.2 Larval Midgut    211.6 Larval Hindgut    175.1 Larval Malpighian Tubules    244.5 Larval Fat Body    286.2 Larval Salivary Gland    216.6 Larval Trachea    202.975 Larval Carcass    143.8 Adult Head    223.2 Adult Eye    241.975 Adult Brain    237.5 Adult Thoracic-Abdominal Ganglion    251.1 Adult Crop    187.8 Adult Midgut    207.6 Adult Hindgut    167.5 Adult Malpighian Tubules    165.8 Adult Fat Body    213.7 Adult Salivary Gland    191.5 Adult Heart    179.25 Adult VirginFemale Spermatheca    242.9 Adult InseminatedFemale Spermatheca    254.8 Adult Ovary    352.2 Adult Testis    29 Adult Male Accessory Gland    145.3 Adult Carcass    149.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    329.2 Larval Midgut    211.6 Larval Hindgut    175.1 Larval Malpighian Tubules    244.5 Larval Fat Body    286.2 Larval Salivary Gland    216.6 Larval Trachea    202.975 Larval Carcass    143.8 Adult Head    223.2 Adult Eye    241.975 Adult Brain    237.5 Adult Thoracic-Abdominal Ganglion    251.1 Adult Crop    187.8 Adult Midgut    207.6 Adult Hindgut    167.5 Adult Malpighian Tubules    165.8 Adult Fat Body    213.7 Adult Salivary Gland    191.5 Adult Heart    179.25 Adult VirginFemale Spermatheca    242.9 Adult InseminatedFemale Spermatheca    254.8 Adult Ovary    352.2 Adult Testis    29 Adult Male Accessory Gland    145.3 Adult Carcass    149.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    329.2 Larval Midgut    211.6 Larval Hindgut    175.1 Larval Malpighian Tubules    244.5 Larval Fat Body    286.2 Larval Salivary Gland    216.6 Larval Trachea    202.975 Larval Carcass    143.8 Adult Head    223.2 Adult Eye    241.975 Adult Brain    237.5 Adult Thoracic-Abdominal Ganglion    251.1 Adult Crop    187.8 Adult Midgut    207.6 Adult Hindgut    167.5 Adult Malpighian Tubules    165.8 Adult Fat Body    213.7 Adult Salivary Gland    191.5 Adult Heart    179.25 Adult VirginFemale Spermatheca    242.9 Adult InseminatedFemale Spermatheca    254.8 Adult Ovary    352.2 Adult Testis    29 Adult Male Accessory Gland    145.3 Adult Carcass    149.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    329.2 Larval Midgut    211.6 Larval Hindgut    175.1 Larval Malpighian Tubules    244.5 Larval Fat Body    286.2 Larval Salivary Gland    216.6 Larval Trachea    202.975 Larval Carcass    143.8 Adult Head    223.2 Adult Eye    241.975 Adult Brain    237.5 Adult Thoracic-Abdominal Ganglion    251.1 Adult Crop    187.8 Adult Midgut    207.6 Adult Hindgut    167.5 Adult Malpighian Tubules    165.8 Adult Fat Body    213.7 Adult Salivary Gland    191.5 Adult Heart    179.25 Adult VirginFemale Spermatheca    242.9 Adult InseminatedFemale Spermatheca    254.8 Adult Ovary    352.2 Adult Testis    29 Adult Male Accessory Gland    145.3 Adult Carcass    149.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    329.2 Larval Midgut    211.6 Larval Hindgut    175.1 Larval Malpighian Tubules    244.5 Larval Fat Body    286.2 Larval Salivary Gland    216.6 Larval Trachea    202.975 Larval Carcass    143.8 Adult Head    223.2 Adult Eye    241.975 Adult Brain    237.5 Adult Thoracic-Abdominal Ganglion    251.1 Adult Crop    187.8 Adult Midgut    207.6 Adult Hindgut    167.5 Adult Malpighian Tubules    165.8 Adult Fat Body    213.7 Adult Salivary Gland    191.5 Adult Heart    179.25 Adult VirginFemale Spermatheca    242.9 Adult InseminatedFemale Spermatheca    254.8 Adult Ovary    352.2 Adult Testis    29 Adult Male Accessory Gland    145.3 Adult Carcass    149.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    329.2 Larval Midgut    211.6 Larval Hindgut    175.1 Larval Malpighian Tubules    244.5 Larval Fat Body    286.2 Larval Salivary Gland    216.6 Larval Trachea    202.975 Larval Carcass    143.8 Adult Head    223.2 Adult Eye    241.975 Adult Brain    237.5 Adult Thoracic-Abdominal Ganglion    251.1 Adult Crop    187.8 Adult Midgut    207.6 Adult Hindgut    167.5 Adult Malpighian Tubules    165.8 Adult Fat Body    213.7 Adult Salivary Gland    191.5 Adult Heart    179.25 Adult VirginFemale Spermatheca    242.9 Adult InseminatedFemale Spermatheca    254.8 Adult Ovary    352.2 Adult Testis    29 Adult Male Accessory Gland    145.3 Adult Carcass    149.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    329.2 Larval Midgut    211.6 Larval Hindgut    175.1 Larval Malpighian Tubules    244.5 Larval Fat Body    286.2 Larval Salivary Gland    216.6 Larval Trachea    202.975 Larval Carcass    143.8 Adult Head    223.2 Adult Eye    241.975 Adult Brain    237.5 Adult Thoracic-Abdominal Ganglion    251.1 Adult Crop    187.8 Adult Midgut    207.6 Adult Hindgut    167.5 Adult Malpighian Tubules    165.8 Adult Fat Body    213.7 Adult Salivary Gland    191.5 Adult Heart    179.25 Adult VirginFemale Spermatheca    242.9 Adult InseminatedFemale Spermatheca    254.8 Adult Ovary    352.2 Adult Testis    29 Adult Male Accessory Gland    145.3 Adult Carcass    149.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    223.2   NA  Eye    241.975   NA  Brain    237.5   329.2  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    251.1   NA  Crop    187.8   211.6  Midgut    207.6   175.1  Hindgut    167.5   244.5  Malpighian Tubules    165.8   286.2  Fat Body    213.7   216.6  Salivary Gland    191.5   NA  Heart    179.25   202.975  Trachea    NA   NA  VirginFemale Spermatheca    242.9   NA  InseminatedFemale Spermatheca    254.8   NA  Ovary    352.2   NA  Testis    29   NA  Male Accessory Gland    145.3   143.8  Carcass    149.3 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    17 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    10 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    17 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    10 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male  26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    17 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    10 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    17 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    10 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    17 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    10 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    17 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    10 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male  26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    17 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    10 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    17 central nervous system, pupae P8    12 head, virgin 1-day female    7 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    10 fat body, larvae L3 wandering    5 fat body, white prepupae    10 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    26 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    8 embryo 04-06hr    20 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    13 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    8 embryo 04-06hr    20 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    13 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    8 embryo 04-06hr    20 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    13 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    8 embryo 04-06hr    20 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    13 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    16 adult female 30day    17 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    8 embryo 04-06hr    20 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    13 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    8 embryo 04-06hr    20 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    13 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    8 embryo 04-06hr    20 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    13 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    22 embryo 02-04hr    8 embryo 04-06hr    20 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    8 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    7 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    12 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    13 pupae 2d postWPP    11 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    16 adult female 30day    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    38 Schneider line 2 Sg4    28 embryonic 1182-4H    25 embryonic GM2    29 embryonic Kc167    21 embryonic S1    36 embryonic S3    20 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    21 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    30 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    22 ovary fGS/OSS    21 ovary OSC    17 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (38) Schneider line 2 Sg4  (28) embryonic 1182-4H    25 embryonic GM2  (29) embryonic Kc167    21 embryonic S1  (36) embryonic S3    20 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    21 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    24 wing disc ML-DmD21  (30) wing disc ML-DmD32    22 haltere disc ML-DmD17-c3  26 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1  (29) CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    22 ovary fGS/OSS    21 ovary OSC    17 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    38 Schneider line 2 Sg4    28 embryonic 1182-4H    25 embryonic GM2    29 embryonic Kc167    21 embryonic S1    36 embryonic S3    20 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    21 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    30 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    22 ovary fGS/OSS    21 ovary OSC    17 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    38 Schneider line 2 Sg4    28 embryonic 1182-4H    25 embryonic GM2    29 embryonic Kc167    21 embryonic S1    36 embryonic S3    20 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    21 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    30 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    22 ovary fGS/OSS    21 ovary OSC    17 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    38 Schneider line 2 Sg4    28 embryonic 1182-4H    25 embryonic GM2    29 embryonic Kc167    21 embryonic S1    36 embryonic S3    20 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    21 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    30 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    22 ovary fGS/OSS    21 ovary OSC    17 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (38) Schneider line 2 Sg4  28 embryonic 1182-4H    25 embryonic GM2  29 embryonic Kc167    21 embryonic S1  (36) embryonic S3    20 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    21 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    24 wing disc ML-DmD21  30 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3  26 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1  29 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    22 ovary fGS/OSS    21 ovary OSC    17 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    38 Schneider line 2 Sg4    28 embryonic 1182-4H    25 embryonic GM2    29 embryonic Kc167    21 embryonic S1    36 embryonic S3    20 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    21 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    30 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    22 ovary fGS/OSS    21 ovary OSC    17 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    38 Schneider line 2 Sg4    28 embryonic 1182-4H    25 embryonic GM2    29 embryonic Kc167    21 embryonic S1    36 embryonic S3    20 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    21 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    30 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    26 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    22 ovary fGS/OSS    21 ovary OSC    17 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    17 Ethanol 5% 3 hrs, larvae L3    14 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    16 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    17 Ethanol 5% 3 hrs, larvae L3    14 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    17 Ethanol 5% 3 hrs, larvae L3    14 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    17 Ethanol 5% 3 hrs, larvae L3    14 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    16 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    17 Ethanol 5% 3 hrs, larvae L3    14 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    16 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    17 Ethanol 5% 3 hrs, larvae L3    14 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    17 Ethanol 5% 3 hrs, larvae L3    14 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    17 Ethanol 5% 3 hrs, larvae L3    14 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    14 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013762 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8203-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Cdk5GD13840 Other Phenotypes Allele neuroanatomy defective | embryonic stage, with Scer\GAL4unspecified Cdk5K33A.Scer\UAS.T:Zzzz\FLAG short lived, with Scer\GAL4elav-C155 Cdk5K33A.Scer\UAS.T:Zzzz\FLAG Phenotype manifest in Allele motor neuron | embryonic stage, with Scer\GAL4unspecified Cdk5K33A.Scer\UAS.T:Zzzz\FLAG presumptive embryonic/larval nervous system, with Scer\GAL4unspecified Cdk5K33A.Scer\UAS.T:Zzzz\FLAG
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Cdk5 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Cdk5 Class Mutagen Stocks Known lesion Cdk5GD13840 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Cdk5GL00189 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Cdk5JF02667 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Cdk5KK109344 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Cdk5+tKa in vitro construct - genomic fragment 0 Yes Cdk5K33A.Scer\UAS.T:Zzzz\FLAG in vitro construct - amino acid replacement 0 Yes Cdk5NIG.8203R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Cdk5Scer\UAS.P\T.cKa in vitro construct - regulatory fusion 0 Yes Cdk5Scer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion 0 Yes Cdk5Δ in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel9026 (Ryder, 2004.4.26) Df(2R)Jp4 (Sauer et al., 1996) Df(2R)Jp5 (Sauer et al., 1996) Df(2R)WMG (Sauer et al., 1996) Df(2R)XTE-18 (Sauer et al., 1996)
Not disrupted in	Df(2R)Jp6 (Sauer et al., 1996) Df(2R)l13 (Sauer et al., 1996) Df(2R)XTE-11 (Sauer et al., 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct M{UASp-Cdk5.K} NA P{GD13840} NA P{KK109344} NA P{NIG.8203R} NA P{TRiP.GL00189} NA P{TRiP.JF02667} NA P{UAS-Cdk5K33A-FLAG} NA P{UAS-Cdk5-FLAG} NA characterization construct P{Cdk5wt} NA P{Flo-1wt.Cdk5Δ.CG30466wt.CG8204wt} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 13 terms )
Molecular Function
CV term Evidence References
cyclin-dependent protein serine/threonine kinase activity inferred from direct assay (Connell-Crowley et al., 2000) protein serine/threonine kinase activity inferred from direct assay (Kang et al., 2012)
Biological Process
CV term Evidence References
adult locomotory behavior inferred from mutant phenotype (Kissler et al., 2009) axonogenesis inferred from genetic interaction with Cdk5α (Connell-Crowley et al., 2000) inferred from mutant phenotype (Connell-Crowley et al., 2000) cell cycle inferred from mutant phenotype (Kiger et al., 2003) determination of adult lifespan inferred from mutant phenotype (Connell-Crowley et al., 2007) flight behavior inferred from mutant phenotype (Kissler et al., 2009) intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress inferred from genetic interaction with ninaE (Kang et al., 2012) melanotic encapsulation of foreign target inferred from mutant phenotype (Howell et al., 2012) motor neuron axon guidance inferred from mutant phenotype (Connell-Crowley et al., 2007) protein phosphorylation inferred from direct assay (Connell-Crowley et al., 2000) regulation of synaptic growth at neuromuscular junction inferred from mutant phenotype (Kissler et al., 2009)
Cellular Component
CV term Evidence References
cyclin-dependent protein kinase 5 holoenzyme complex inferred from physical interaction with Cdk5α (Connell-Crowley et al., 2000)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) cyclin binding non-traceable author statement (Swiss-Prot Project Members, 1996.2.1) cyclin-dependent protein serine/threonine kinase activity inferred from sequence or structural similarity (Hellmich et al., 1994) inferred from sequence or structural similarity with Saccharomyces CDC28 (FlyBase, 1992-) protein serine/threonine kinase activity non-traceable author statement (Lehner, 1996.7.23, Morrison et al., 2000, Swiss-Prot Project Members, 1996.2.1)
Biological Process
CV term Evidence References
neurotransmitter secretion non-traceable author statement (Bellen, 2000.12.25) protein phosphorylation inferred from sequence or structural similarity (Hellmich et al., 1994) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Cdk5 - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - CG10116 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - CG2021 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - CG3735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - Fmr1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - Hsp23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - Imp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - Mekk1 enzymatic study, western blot, anti bait coimmunoprecipitation, anti tag western blot (Kang et al., 2012) Cdk5 - Rrp46 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - SmD3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - SmG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - TFAM anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - Trap1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - ctp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - eIF4AIII anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - mRpL42 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - mRpL43 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - mRpL52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - mRpS28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - mTerf3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk5 - snama anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Cdk5 allele Gene References Cdk5K33A.Scer\UAS.T:Zzzz\FLAG Cdk5α (Connell-Crowley et al., 2000) Cdk5Scer\UAS.T:Zzzz\FLAG Cdk5α (Connell-Crowley et al., 2000, Shulman and Feany, 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62461 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0013762 DroID - A comprehensive database of gene and protein interactions. • FBgn0013762 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36727 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P48609
Orthologs
OrthoDB Orthologs (68) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6QZ862 Dipteran inclusive ortholog search EOG6QRHCT Insect inclusive ortholog search EOG6MKMC3 Arthropod inclusive ortholog search EOG647D90 Metazoa inclusive ortholog search EOG6PGJT5
Orthologs in Drosophila Species (EOG6QZ862)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Cdk5     Drosophila simulans   Dsim\GD25609 Y   Drosophila sechellia   Dsec\GM20132 Y   Drosophila erecta   Dere\GG22344 Y   Drosophila yakuba   Dyak\GE14145 Y   Drosophila ananassae   Dana\GF22626 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20894 Y   Drosophila persimilis   Dper\GL11507 Y   Drosophila willistoni   Dwil\GK21950 Y   Drosophila virilis   Dvir\GJ21207 Y   Drosophila mojavensis   Dmoj\GI19456 Y   Drosophila grimshawi   Dgri\GH20285 Y
Orthologs in non-Drosophila Dipterans (EOG6QRHCT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008648   Anopheles gambiae Malaria mosquito  Agam\AGAP005772   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000065
Orthologs in non-Dipteran Insects (EOG6MKMC3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Cdk5   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG010706   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10283   Atta cephalotes Leafcutter ant  Acep\ACEP20868   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL12653   Linepithema humile Argentine ant  Lhum\LH10988   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17952   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001995   Bombyx mori Silkmoth  Bmor\BGIBMGA004018   Pediculus humanus Human body louse  Phum\PHUM263550   Tribolium castaneum Red flour beetle  Tcas\TC009740
Orthologs in non-Insect Arthropods (EOG647D90)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_299705   Ixodes scapularis Deer tick  Isca\ISCW021518
Orthologs in non-Arthropod Metazoa (EOG6PGJT5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ZC123.4 Y Caenorhabditis elegans Nematode  Cele\cdk-5 Y Caenorhabditis elegans Nematode  Cele\pct-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016385gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003654gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017705gn Y Danio rerio Zebrafish  Drer\cdk15 Y Danio rerio Zebrafish  Drer\cdk16 Y Danio rerio Zebrafish  Drer\zgc:172096 Y Danio rerio Zebrafish  Drer\ENSDARG00000012204 Y Danio rerio Zebrafish  Drer\cdk5 Y Danio rerio Zebrafish  Drer\ENSDARG00000063721 Y Danio rerio Zebrafish  Drer\cdk16 Y Xenopus tropicalis Western clawed frog  Xtro\cdk16 Y Xenopus tropicalis Western clawed frog  Xtro\cdk14 Y Xenopus tropicalis Western clawed frog  Xtro\cdk17 Y Xenopus tropicalis Western clawed frog  Xtro\cdk5 Y Xenopus tropicalis Western clawed frog  Xtro\cdk15 Y Xenopus tropicalis Western clawed frog  Xtro\cdk18 Y Gallus gallus Domestic chicken  Ggal\CDK17 Y Gallus gallus Domestic chicken  Ggal\CDK14 Y Gallus gallus Domestic chicken  Ggal\CDK15 Y Gallus gallus Domestic chicken  Ggal\CDK18 Y Mus musculus House mouse  Mmus\Cdk5 Y Mus musculus House mouse  Mmus\Cdk18 Y Mus musculus House mouse  Mmus\Cdk16 Y Mus musculus House mouse  Mmus\Cdk14 Y Mus musculus House mouse  Mmus\Cdk17 Y Mus musculus House mouse  Mmus\Cdk15 Y Rattus norvegicus Norway rat  Rnor\Fzd7 Y Rattus norvegicus Norway rat  Rnor\Cdk5 Y Rattus norvegicus Norway rat  Rnor\Cdk17 Y Rattus norvegicus Norway rat  Rnor\Cdk18 Y Rattus norvegicus Norway rat  Rnor\Pftk1_predicted Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000008578 Y Homo sapiens Human  Hsap\CDK16 Y Homo sapiens Human  Hsap\CDK5 Y Homo sapiens Human  Hsap\CDK17 Y Homo sapiens Human  Hsap\CDK14 Y Homo sapiens Human  Hsap\ENSG00000117266 Y
Human Orthologs (5)
Gene OMIM HGNC Hsap\CDK16     Hsap\CDK5     Hsap\CDK17     Hsap\CDK14     Hsap\ENSG00000117266
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD25609 Drosophila sechellia Dsec\GM20132 Drosophila erecta Dere\GG22344 Drosophila yakuba Dyak\GE14145 Drosophila ananassae Dana\GF22626 Drosophila pseudoobscura pseudoobscura Dpse\GA20894 Drosophila persimilis Dper\GL11507 Drosophila willistoni Dwil\GK21950 Drosophila virilis Dvir\GJ21207 Drosophila mojavensis Dmoj\GI19456 Drosophila grimshawi Dgri\GH20285
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	27517 y1 v1; P{TRiP.JF02667}attP2 35287 y1 sc* v1; P{TRiP.GL00189}attP2
VDRC	v104491 P{KK109344}VIE-260B v35855 w1118; P{GD13840}v35855 v35856 w1118; P{GD13840}v35856
Genomic Clones ( 1 )
	BACR48C01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 18 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD01910
Other clones	DPiM_LD01910
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RH12210 RH16221 RH53104 RH56180 RH60443 SD23579
Other clones	173095 174662 214503.76 CK01.125D.E4 EC052536 EK275528 EK277452 FGM319F10 IP14993 RH15206
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013762 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36727
Antibody Information
	polyclonal (Sauer et al., 1996)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Cdk5 CG8203 (Li, 1999.11)
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) Promoter structures of Flo abut to or overlap with the 3'UTR of Cdk5. Overall orientation not stated: Cdk5+ Flo+ (Hoehne et al., 1999) Characterisation of Cdk5. (Sauer et al., 1996) Isolated from a genomic library, using a rat CDK5 cDNA as a probe. (Hellmich et al., 1994) Cdk5 has been cloned and sequenced. (Hellmich et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057732 RefSeq (Proteins) • NP_477080 DDBJ / EMBL / GenBank • AA816484 AAA63754 AAF58119 AAL28597 AC006242 AC008369 AW941483 AY061049 CAA67861 U21552 X99511 Entrez Gene - A searchable database of RefSeq genes. • 36727 UniProtKB/Swiss-Prot • P48609
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62461 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0013762 DroID - A comprehensive database of gene and protein interactions. • FBgn0013762 DRSC - Results from RNAi screens. • FBgn0013762 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013762 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8203-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013762 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36727 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/cdk5-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36727 modMine - Data generated by the modENCODE project. • FBgn0013762 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P48609
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CDK5 (Shulman and Feany, 2003, Rascle et al., 2003, Bellen, 2000.12.25, Hellmich, 1995.2.23, Choe et al., 2007) cdk5 (Lehner, 1996.7.23, Hoehne et al., 1999, Sauer et al., 1996, Kang et al., 2012, Kissler et al., 2009, Mudiganti et al., 2010) Cdk5 (Kissler and Suter, 2007, Connell-Crowley et al., 2000, Connell-Crowley et al., 2007, Chau et al., 2006, Ben-Shahar et al., 2007, Lin et al., 2004, Lin et al., 2007, Chatterjee et al., 2009, Howell et al., 2012, Japanese National Institute of Genetics, 2012.5.21) CG8203 (Morrison et al., 2000, Verstreken and Bellen, 2000.9.4, Rogers et al., 2003, Keleman et al., 2009.8.5, Haussmann et al., 2008, Perkins et al., 2009, Ni et al., 2011.7.19, Howell et al., 2012, Kissler et al., 2009) DmCdk5 (Kolonin and Finley, 1998)
Name Synonym	Cyclin-dependent kinase 5 (Rogers et al., 2003, Kissler et al., 2009) cyclin-dependent kinase 5 (Chau et al., 2006)
Secondary FlyBase IDs
References ( 63 )
Generate a list of	All references relating to this gene ( 63 )
List References by type	Research paper  ( 26 ) Supplementary material  ( 9 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 8 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Letter  ( 1 )
Recent research papers ( 5 )
	Howell et al., 2012, Immunogenetics 64(2): 155--161 A directed miniscreen for genes involved in the Drosophila anti-parasitoid immune response. [FBrf0217123] Kang et al., 2012, Nat. Cell Biol. 14(4): 409--415 CDK5 and MEKK1 mediate pro-apoptotic signalling following endoplasmic reticulum stress in an autosomal dominant retinitis pigmentosa model. [FBrf0217924] Prithviraj et al., 2012, J. Vis. Exp.: Ex vivo Culturing of Whole, Developing Drosophila Brains. [FBrf0219105] Trunova and Giniger, 2012, Dis. Model Mech. 5(2): 210--219 Absence of the Cdk5 activator p35 causes adult-onset neurodegeneration in the central brain of Drosophila. [FBrf0217617] Trunova et al., 2011, J. Neurosci. 31(29): 10451--10462 Cdk5 regulates the size of an axon initial segment-like compartment in mushroom body neurons of the Drosophila central brain. [FBrf0214449] Show all research papers ...