FlyBase Gene Report: Dmel\Cdk5

General Information

Symbol

Dmel\Cdk5

Species

D. melanogaster

Name

Cyclin-dependent kinase 5

Annotation Symbol

CG8203

Feature Type

protein_coding_gene

FlyBase ID

FBgn0013762

Gene Model Status

Current

Stock Availability

5 publicly available

Enzyme Name (EC)

Cyclin-dependent kinase (2.7.11.22)

Gene Summary

Cyclin-dependent kinase 5 (Cdk5) encodes a protein serine-threonine kinase of the cyclin-dependent kinase family whose activity is largely restricted to post-mitotic neurons. It is required for synaptic homeostasis and fidelity of axon patterning, and altered activity causes adult-onset neurodegeneration. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

52A13-52A13

Sequence location

2R:15,569,528..15,571,417 [-]

Recombination map

2-74

RefSeq locus

NT_033778 REGION:15569528..15571417

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables cyclin-dependent protein serine/threonine kinase activity

inferred from direct assay

(Connell-Crowley et al., 2000)

enables protein serine/threonine kinase activity

inferred from direct assay

(Kang et al., 2012)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables cyclin-dependent protein serine/threonine kinase activity

inferred from sequence or structural similarity

(Hellmich et al., 1994)

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (13 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in adult locomotory behavior

inferred from mutant phenotype

(Kissler et al., 2009)

involved_in axonogenesis

inferred from mutant phenotype

(Connell-Crowley et al., 2000)

inferred from genetic interaction with FLYBASE:Cdk5α; FB:FBgn0027491

(Connell-Crowley et al., 2000)

involved_in centrosome localization

inferred from mutant phenotype

(Jauffred et al., 2013)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Connell-Crowley et al., 2007)

involved_in flight behavior

inferred from mutant phenotype

(Kissler et al., 2009)

involved_in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress

inferred from genetic interaction with FLYBASE:ninaE; FB:FBgn0002940

(Kang et al., 2012)

involved_in melanotic encapsulation of foreign target

inferred from mutant phenotype

(Howell et al., 2012)

involved_in motor neuron axon guidance

inferred from mutant phenotype

(Connell-Crowley et al., 2007)

involved_in protein phosphorylation

inferred from direct assay

(Connell-Crowley et al., 2000)

involved_in regulation of synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(Kissler et al., 2009)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in axonogenesis

inferred from biological aspect of ancestor with PANTHER:PTN000623852

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in neuron apoptotic process

inferred from biological aspect of ancestor with PANTHER:PTN000623852

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein phosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity

(Hellmich et al., 1994)

involved_in regulation of transcription involved in G1/S transition of mitotic cell cycle

inferred from biological aspect of ancestor with PANTHER:PTN002373304

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in synaptic vesicle transport

inferred from biological aspect of ancestor with PANTHER:PTN000623852

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of protein kinase 5 complex

inferred from physical interaction with FLYBASE:Cdk5α; FB:FBgn0027491

(Connell-Crowley et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002373304

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CYCLIN DEPENDENT KINASES

Protein Family (UniProt)

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. (P48609)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

ATP + a protein = ADP + a phosphoprotein (2.7.11.22)

Predictions / Assertions

ATP + a protein = ADP + a phosphoprotein (2.7.11.22)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CYCLIN DEPENDENT KINASES -: Cyclin-dependent kinases (CDKs) are serine/threonine kinases whose activity depends on a regulatory cyclin subunit. (Adapted from PMID:25180339).

Protein Function (UniProtKB)

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Possible regulator of neuronal differentiation and/or development (By similarity).

(UniProt, P48609)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Cdk5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cdk5-RA

FBtr0087350

NM_057732

1604

294

Additional Transcript Data and Comments

Reported size (kB)

1.6 (northern blot)

(Sauer et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Cdk5-PA

FBpp0086483

33.2

294

7.82

NP_477080

AAF58119

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

294 (aa)

(Sauer et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P48609

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cdk5 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cdk5_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval central nervous system

(Sauer et al., 1996)

presumptive embryonic/larval nervous system

(Sauer et al., 1996)

organism

(Sauer et al., 1996)

embryonic stage -- adult stage

(Sauer et al., 1996)

Additional Descriptive Data

Cdk5 transcripts are detected at all stages of development on northern blots. They are found to be ubiquitous in early embryos by in situ hybridization. They are also present at a low level throughout the embryo at later stages of embryogenesis. Stronger signals are observed in the CNS of late embryos.

(Sauer et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Sauer et al., 1996)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Constant levels of Cdk5 protein are observed through embryogenesis by immunoblotting.

(Sauer et al., 1996)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of protein kinase 5 complex

inferred from physical interaction with FLYBASE:Cdk5α; FB:FBgn0027491

(Connell-Crowley et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Cdk5 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cdk5

View exonic expression by tissue for Dmel\Cdk5

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB

36727/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0013762

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8203-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8203

Flygut - An atlas of the Drosophila adult midgut

FBgn0013762

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cdk5

Transgenic constructs containing regulatory region of Cdk5

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene disrupted in

Df(2R)Exel9026

(Ryder, 2004.4.26)

Df(2R)Jp4

(Sauer et al., 1996)

Df(2R)Jp5

(Sauer et al., 1996)

Df(2R)WMG

(Sauer et al., 1996)

Df(2R)XTE-18

(Sauer et al., 1996)

Gene not disrupted in

Df(2R)Jp6

(Sauer et al., 1996)

Df(2R)l13

(Sauer et al., 1996)

Df(2R)XTE-11

(Sauer et al., 1996)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Cdk5^GD13840

viable, with Scer\GAL4^Mef2.PR

Cdk5^GD13840

viable, with Scer\GAL4^pnr-MD237

Cdk5^GD13840

viable, with Scer\GAL4^tin.CΔ4

Cdk5^GD13840

Sterility

Allele

female semi-sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

Cdk5^GL00189

Other Phenotypes

Allele

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^unspecified

Cdk5^{K33A.UAS.Tag:FLAG}

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Cdk5^GD13840

short lived, with Scer\GAL4^elav-C155

Phenotype manifest in

Allele

centrosome, with Scer\GAL4^neur-P72

Cdk5^KK109344

external sensory organ precursor cell I, with Scer\GAL4^neur-P72

Cdk5^KK109344

motor neuron | embryonic stage, with Scer\GAL4^unspecified

Cdk5^{K33A.UAS.Tag:FLAG}

presumptive embryonic/larval nervous system, with Scer\GAL4^unspecified

Cdk5^{K33A.UAS.Tag:FLAG}

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CDK5

14 of 15

Yes

4 of 15

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cdk5

14 of 15

Yes

4 of 15

4 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Rattus norvegicus (Norway rat) (8)

Rnor\Cdk5

13 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Xenopus tropicalis (Western clawed frog) (8)

Xtro\cdk5

9 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Danio rerio (Zebrafish) (9)

Drer\cdk5

12 of 15

Yes

4 of 15

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

Drer\si:dkey-166c18.1

2 of 15

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\cdk-5

13 of 15

Yes

4 of 15

3 of 15

2 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (5)

Atha\CDC2

2 of 9

Yes

Atha\CDKB1;2

2 of 9

Yes

Atha\CDKB2;2

2 of 9

Yes

Atha\CDKB1;1

1 of 9

Yes

Atha\CDKB2;1

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\PHO85

7 of 15

Yes

Scer\CDC28

4 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\pef1

7 of 12

Yes

Spom\cdc2

3 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190C37 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20894

Drosophila persimilis

Dper\GL11507

Drosophila willistoni

Dwil\GK21950

Drosophila virilis

Dvir\GJ21207

Drosophila mojavensis

Dmoj\GI19456

Drosophila grimshawi

Dgri\GH20285

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091507QM )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17027504

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_03204

Mayetiola destructor

Hessian fly

Mdes\Mdes006285

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL018160

Anopheles darlingi

American malaria mosquito

Adar\ADAC010317

Anopheles gambiae

Malaria mosquito

Agam\AGAP005772

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ000065

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0862 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA004018

Danaus plexippus

Monarch butterfly

Dple\DPOGS211766-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL007938

Apis florea

Little honeybee

Aflo\2163579412

Apis mellifera

Western honey bee

Amel\Cdk5

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP22833

Bombus terrestris

Buff-tailed bumblebee

Bter\15210498

Linepithema humile

Argentine ant

Lhum\LH10988

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0000f0

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG010706

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_11349

Tribolium castaneum

Red flour beetle

Tcas\TC009740

Pediculus humanus

Human body louse

Phum\PHUM263550

Rhodnius prolixus

Kissing bug

Rpro\RPRC013655

Cimex lectularius

Bed bug

Clec\CLEC012938

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001995

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_16588

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X081V )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR010000

Ixodes scapularis

Black-legged tick

Isca\ISCW021518

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78113.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g11790

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_299705

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0CH0 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdk1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pctaire

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdk2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdk5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_001227gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pftaire-1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cdk2_1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000006693

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005978

Ciona intestinalis

Vase tunicate

Cint\LOC100182544

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009174

Gallus gallus

Domestic chicken

Ggal\CDK17

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027196

Gallus gallus

Domestic chicken

Ggal\CDK14

Gallus gallus

Domestic chicken

Ggal\CDK15

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028442

Gallus gallus

Domestic chicken

Ggal\CDC2

Gallus gallus

Domestic chicken

Ggal\CDK3

Gallus gallus

Domestic chicken

Ggal\CDK18

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (14)

Gene Symbol

Score

Source

Alignment

6 of 10

6 of 10

4 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CDK5; cyclin dependent kinase 5

model of lissencephaly 7 with cerebellar hypoplasia

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

Cdk5^UAS.Tag:FLAG

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Saito et al., 2019)

Cdk5^GD13840

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}

(Liman et al., 2014)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Liman et al., 2014)

ameliorates retinal disease

modeled by ninaE^G69D.UAS

(Kang et al., 2012)

Cdk5^KK109344

ameliorates retinitis pigmentosa

modeled by ninaE^G69D.UAS

(Chow et al., 2016)

Cdk5^NIG.8203R

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Saito et al., 2019)

Cdk5^{dsRNA.UAS.cUa}

ameliorates brain cancer

modeled by brat^{dsRNA.UAS.cUa}

(Mukherjee et al., 2018)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CDK5; cyclin dependent kinase 5

14 of 15

123831

LISSENCEPHALY 7 WITH CEREBELLAR HYPOPLASIA; LIS7

lissencephaly 7 with cerebellar hypoplasia

CDK2; cyclin dependent kinase 2

4 of 15

116953

CDK1; cyclin dependent kinase 1

3 of 15

116940

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Cdk5 - Cdk5α

pull down, autoradiography

(Connell-Crowley et al., 2000)

Cdk5 - Mekk1

anti bait coimmunoprecipitation, anti tag western blot, enzymatic study, western blot

(Kang et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Cdk5α

enhanceable

Cdk5

(Connell-Crowley et al., 2000)

Cdk5α

suppressible

Cdk5

(Connell-Crowley et al., 2000)

ninaE

suppressible

Cdk5

(Chow et al., 2016, Kang et al., 2012)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62461

DroID - A comprehensive database of gene and protein interactions.

FBgn0013762

MIST (genetic) - An integrated Molecular Interaction Database

Cdk5

MIST (protein-protein) - An integrated Molecular Interaction Database

Cdk5

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-74

Cytogenetic map

52A13-52A13

Sequence location

2R:15,569,528..15,571,417 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

52A13-52A13

Limits computationally determined from genome sequence between P{lacW}Vha36^k07207 and P{lacW}l(2)k05713^k05713

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

52A-52A

(Lehner, 1996.7.23)

52A14-52C9

(determined by in situ hybridisation)

(Hellmich et al., 1994)

Experimentally Determined Recombination Data

Location

2-74

(FlyBase, 2016)

2-78.7

(Comeron et al., 2012)

(Lehner, 1996.7.23)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Bloomington

27517

y¹ v¹; P{TRiP.JF02667}attP2

35287

y¹ sc^* v¹ sev²¹; P{TRiP.GL00189}attP2

VDRC

v104491

P{KK109344}VIE-260B

v35855

w¹¹¹⁸; P{GD13840}v35855

v342015

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01369}attP40/CyO-GFP

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (18)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD01910

Other clones

DPiM_LD01910

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0013762

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0013762

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36727

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Sauer et al., 1996)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Cdk5 CG8203

(Li, 1999.11)

Source for database merge of

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

Promoter structures of Flo abut to or overlap with the 3'UTR of Cdk5. Overall orientation not stated: Cdk5+ Flo+

(Hoehne et al., 1999)

Characterisation of Cdk5.

(Sauer et al., 1996)

Isolated from a genomic library, using a rat CDK5 cDNA as a probe.

(Hellmich et al., 1994)

Cdk5 has been cloned and sequenced.

(Hellmich et al., 1994)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36727

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P48609

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0013762

DRscDB

36727/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0013762

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cdk5_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8203

Flygut - An atlas of the Drosophila adult midgut

FBgn0013762

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36727

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009740

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8203

MARRVEL_MODEL

36727

modMine - A data warehouse for the modENCODE project

FBgn0013762

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P48609

Linkouts

BioGRID - A database of protein and genetic interactions.

62461

DroID - A comprehensive database of gene and protein interactions.

FBgn0013762

DRSC - Results frm RNAi screens

FBgn0013762

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8203-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0013762

FlyMine - An integrated database for Drosophila genomics

FBgn0013762

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

Cdk5

MIST (protein-protein) - An integrated Molecular Interaction Database

Cdk5

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CDK5

(Palu et al., 2020, Bouleau and Tricoire, 2015, Ryoo, 2015, Choksi et al., 2014, Lin et al., 2010, Choe et al., 2007, Rascle et al., 2003, Shulman and Feany, 2003, Bellen, 2000.12.25, Hellmich, 1995.2.23)

CG8203

(Hosono et al., 2015, Howell et al., 2012, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Kissler et al., 2009, Perkins et al., 2009, Haussmann et al., 2008, Rogers et al., 2003, Morrison et al., 2000, Verstreken and Bellen, 2000.9.4)

DmCdk5

(Kolonin and Finley, 1998)

cdk5

(Di Cara et al., 2015, Jauffred et al., 2013, Kang et al., 2012, Mudiganti et al., 2010, Kissler et al., 2009, Hoehne et al., 1999, Lehner, 1996.7.23, Sauer et al., 1996)

Name Synonyms

Cyclin-dependent kinase 5

(Liman et al., 2014, Kissler et al., 2009, Rogers et al., 2003)

cyclin-dependent kinase 5

(Engel et al., 2016, Chau et al., 2006)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (105)

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