• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Chi

General Information
Symbol	Dmel\Chi	Species	D. melanogaster
Name	Chip	Annotation symbol	CG3924
Feature type	protein_coding_gene	FlyBase ID	FBgn0013764
Gene Model Status	Current	Stock availability	12 publicly available
Also Known As	Chip, dLdb, Ldb
Genomic Location
Chromosome (arm)	2R	Recombination map	2-106.8
Cytogenetic map	60B5-60B5	Sequence location	2R:19,919,062..19,921,459 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Chip is referred to in FlyBase by the symbol Dmel\Chi (CG3924, FBgn0013764). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; transcription factor binding; RNA polymerase II transcription factor binding. There is experimental evidence that it is involved in the biological process: chaeta development; regulation of eclosion; axon guidance; transcription, DNA-dependent; nervous system development; chaeta morphogenesis; positive regulation of transcription, DNA-dependent; imaginal disc-derived wing morphogenesis; dendrite guidance; phagocytosis, engulfment. 36 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; synaptic neuropil subdomain; antennal segment; adult segment; adult mesothoracic segment; eo support cell; embryonic/larval neuron; postalar bristle; appendage segment; subesophageal ganglion; unguis. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: LIM binding protein. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1627894_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Chi. Gene sequence location is 2R:19919062..19921459.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features BKN28451 HFA04602 Controlled Vocabulary Terms protein binding transcription factor binding
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 60B5-60B5   Limits computationally determined from genome sequence between P{lacW}Phmk07623&P{lacW}tsrk05633 and P{EP}EP503
Experimentally Determined Cytological Location
Cytogenetic map Notes References 60B1-60B2     (Morcillo, 1997.6.19) 60A-60A   (determined by in situ hybridisation)   (Fernandez-Funez et al., 1998) 60A-60A   (determined by in situ hybridisation)   (Botas, 1998.3.13) 60B1-60B2     (Beaton, 1997.12.27)
Experimentally Determined Recombination Data
Location	2-106.8   2-106 (Morcillo, 1996.4.28) 2-107 (Morcillo et al., 1996) 2- (Morcillo, 1997.6.19)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Chi for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Chi-RA FBtr0072191  NM_057734   2148   577 Chi-RB FBtr0072192  NM_166650   2124   596
Additional Transcript Data & Comments
Reported size (kB)	2.2 (northern blot) (Morcillo et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Chi-PA   FBpp0072100   62.6   577   8.29     NP_477082   AAF47142 Chi-PB   FBpp0072101   64.8   596   7.99     NP_726403   AAM68293
Additional Polypeptide Data & Comments
Reported size (kDa)	577 (aa) (Morcillo et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • LIM binding protein (IPR002691)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202781   AA391318   AA392866   AA439064   AA439366   AA440562   AA539753   AA698924   AA735580   AA816776   AA941101   AA941151   AA941219   AA941801   AA942073   AA979180   AE013599 AAF47142   AE013599 AAM68293   AC004642   AF010325 AAB62574   AI061769   AI061770   AI062093   AI256881   AI405737   AI454973   AI456460   AI456461   AI456804   AI512817   AI514830   AI515011   AI515030   AI515059   AI515220   AI516623   AI520582   AI530760   AI533881   AI542583   AI543074   AW942718   AY058561 AAL13790   BF489020   BF493242   BG638900   BG638912   BG641432   BI164103   BI165809   BI214758   BI232174   BI236867   BI237027   BI362778   BI616220   BI638235   BI640252   BP551206   BT125015   CK132539   CL527848   CO264561   CO264633   CO276775   CO294052   CO319181   CO320775   CO322111   CO342924   CZ472844   EC060165   EC060928   EC063018   EC065280   EC075373   EC198476   EC209890   EC250607   EC259507   EL871847   EL871979   EL872074   EV577260   EV596746   EV597147   GH739348   GH748516   GH748767   GH752495   GH753076   GH754170   GH755431   GH758012   GH758316   GH760606   GH773369   GH775859   GH776595   GH776764   GH780082   GH780427   GH781900   GH782436   GH783865   GH783986   GH786843   GH790121   GH790350   GH791050   GH791217   GH791853   GH792310   GH792871   GH792962   GH793485   GH793764   GH795666   GH795752   GH796104   GH798358   GH798915   GH802213   GH803445   GH804206   GH804338   GH804615   GH805813   GH806253   GH807107   GH808967   GH809069   GH809662   GH810827   GH812623   GH812897   GH813695   GH813795   GH814365   GH815285   GH815653   GH819241   GH820626   GH822347   GH822392   GH822842   GH823804   GH825757   GH830020   GH831403   GH831531   GH833286   GH833727   GH834883   GH837183   GH837711   GH838169   GH838432   GH841085   GH843722   GH846513   GH846795   GH848375   GH849643   GH849675   GH849799   GH850668   GH852813   GH856561   GH856838   GH857972   GH858232   GH859295   GH860235   GH860588   GH863550   GH869266   GH869671   GH869938   GH870985   GH871035   GH871710   GH877107   GH877229   GH877261   GH877269   GH881918   GH890635   GH891032   GH891688   GH891855   GH895683   GH896273   GH897023   GH897052   GH897422   GH898531   GH901317   GH901601   GH904097   GH905037   GH907271   GH907810   GH909011   GH909614   GH910179   GH913246   GH914847   GH915462   GH916937   GH917846   GH918056   GH918215   GH919397   GH920069   GH920082   GH922105   GH922384   GH922949   GH922969   GH923367   GH924449   GH924471   GH925365   GH925495   GH931631   GH934403   GH934432   GH936324   GH936882   GH936983   GH938379   GH939118   GH939299   GH940483   GH940706
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O18353 Q7KVG9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Morcillo et al., 1997)
Additional Descriptive Data
	Chi transcript is expressed in all developmental stages. Expression in early embryos (0-30 min) is tenfold higher than in later developmental stages. (Morcillo et al., 1997)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage & larval stage     • nucleus (Morcillo et al., 1997) larval stage   embryonic/larval salivary gland   (Morcillo et al., 1997) imaginal disc   (Morcillo et al., 1997) embryonic/larval fat body   (Morcillo et al., 1997) pupal stage P5(ii)   antennal lobe projection neuron | subset   (Komiyama and Luo, 2007) adult stage   adult antennal lobe projection neuron DL1 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron D adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VA3 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VA1d adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VM7 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VM2 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron DM6 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VA1lm adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron DA1 lPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron DL3 lPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VA7m lPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VA5 lPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron DM5 lPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron DM2 lPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron DM1 lPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VA1lm vPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron DA1 vPN   (Komiyama and Luo, 2007) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
	Chi protein is detected in embryonic and larval cell nuclei. Staining is also detected along salivary gland chromosomes. (Morcillo et al., 1997) Chi is expressed in many cells surrounding the antennal lobe at 18hrs APF. (Komiyama and Luo, 2007)
Marker for
Subcellular Localization
CV Term	nucleus chromosome (Morcillo et al., 1997)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0013764    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0013764 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0013764 expression level Developmental Stage   Expression Level embryo 00-02hr    7565 embryo 02-04hr    8875 embryo 04-06hr    5169 embryo 06-08hr    6352 embryo 08-10hr    5703 embryo 10-12hr    6246 embryo 12-14hr    3451 embryo 14-16hr    5253 embryo 16-18hr    3629 embryo 18-20hr    2353 embryo 20-22hr    3137 embryo 22-24hr    2427 larva L1    1783 larva L2    1368 larva L3 12hr old    1212 larva L3 puffstage 1-2    1339 larva L3 puffstage 3-6    2060 larva L3 puffstage 7-9    2043 white prepupae new    2088 white prepupae 12hr    2475 white prepupae 24hr    3123 pupae 2d postWPP    2215 pupae 3d postWPP    1247 pupae 4d postWPP    1317 adult male 01day    1513 adult male 05day    2045 adult male 30day    2105 adult female 01day    2657 adult female 05day    3015 adult female 30day    3455 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (7565) embryo 02-04hr  (8875) embryo 04-06hr  (5169) embryo 06-08hr  (6352) embryo 08-10hr  (5703) embryo 10-12hr  (6246) embryo 12-14hr  (3451) embryo 14-16hr  (5253) embryo 16-18hr  (3629) embryo 18-20hr  (2353) embryo 20-22hr  (3137) embryo 22-24hr  (2427) larva L1  (1783) larva L2  (1368) larva L3 12hr old  (1212) larva L3 puffstage 1-2  (1339) larva L3 puffstage 3-6  (2060) larva L3 puffstage 7-9  (2043) white prepupae new  (2088) white prepupae 12hr  (2475) white prepupae 24hr  (3123) pupae 2d postWPP  (2215) pupae 3d postWPP  (1247) pupae 4d postWPP  (1317) adult male 01day  (1513) adult male 05day  (2045) adult male 30day  (2105) adult female 01day  (2657) adult female 05day  (3015) adult female 30day  (3455) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (7565) embryo 02-04hr  (8875) embryo 04-06hr  (5169) embryo 06-08hr  (6352) embryo 08-10hr  (5703) embryo 10-12hr  (6246) embryo 12-14hr  (3451) embryo 14-16hr  (5253) embryo 16-18hr  (3629) embryo 18-20hr  (2353) embryo 20-22hr  (3137) embryo 22-24hr  (2427) larva L1  (1783) larva L2    1368 larva L3 12hr old    1212 larva L3 puffstage 1-2    1339 larva L3 puffstage 3-6  (2060) larva L3 puffstage 7-9  (2043) white prepupae new  (2088) white prepupae 12hr  (2475) white prepupae 24hr  (3123) pupae 2d postWPP  (2215) pupae 3d postWPP    1247 pupae 4d postWPP    1317 adult male 01day  (1513) adult male 05day  (2045) adult male 30day  (2105) adult female 01day  (2657) adult female 05day  (3015) adult female 30day  (3455) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    7565 embryo 02-04hr    8875 embryo 04-06hr    5169 embryo 06-08hr    6352 embryo 08-10hr    5703 embryo 10-12hr    6246 embryo 12-14hr    3451 embryo 14-16hr    5253 embryo 16-18hr    3629 embryo 18-20hr    2353 embryo 20-22hr    3137 embryo 22-24hr    2427 larva L1    1783 larva L2    1368 larva L3 12hr old    1212 larva L3 puffstage 1-2    1339 larva L3 puffstage 3-6    2060 larva L3 puffstage 7-9    2043 white prepupae new    2088 white prepupae 12hr    2475 white prepupae 24hr    3123 pupae 2d postWPP    2215 pupae 3d postWPP    1247 pupae 4d postWPP    1317 adult male 01day    1513 adult male 05day    2045 adult male 30day    2105 adult female 01day    2657 adult female 05day    3015 adult female 30day    3455 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    7565 embryo 02-04hr    8875 embryo 04-06hr    5169 embryo 06-08hr    6352 embryo 08-10hr    5703 embryo 10-12hr    6246 embryo 12-14hr    3451 embryo 14-16hr    5253 embryo 16-18hr    3629 embryo 18-20hr    2353 embryo 20-22hr    3137 embryo 22-24hr    2427 larva L1    1783 larva L2    1368 larva L3 12hr old    1212 larva L3 puffstage 1-2    1339 larva L3 puffstage 3-6    2060 larva L3 puffstage 7-9    2043 white prepupae new    2088 white prepupae 12hr    2475 white prepupae 24hr    3123 pupae 2d postWPP    2215 pupae 3d postWPP    1247 pupae 4d postWPP    1317 adult male 01day    1513 adult male 05day    2045 adult male 30day    2105 adult female 01day    2657 adult female 05day    3015 adult female 30day    3455 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0013764 expression level Developmental Stage   Expression Level embryo 00-02hr    7565 embryo 02-04hr    8875 embryo 04-06hr    5169 embryo 06-08hr    6352 embryo 08-10hr    5703 embryo 10-12hr    6246 embryo 12-14hr    3451 embryo 14-16hr    5253 embryo 16-18hr    3629 embryo 18-20hr    2353 embryo 20-22hr    3137 embryo 22-24hr    2427 larva L1    1783 larva L2    1368 larva L3 12hr old    1212 larva L3 puffstage 1-2    1339 larva L3 puffstage 3-6    2060 larva L3 puffstage 7-9    2043 white prepupae new    2088 white prepupae 12hr    2475 white prepupae 24hr    3123 pupae 2d postWPP    2215 pupae 3d postWPP    1247 pupae 4d postWPP    1317 adult male 01day    1513 adult male 05day    2045 adult male 30day    2105 adult female 01day    2657 adult female 05day    3015 adult female 30day    3455 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (7565) embryo 02-04hr  (8875) embryo 04-06hr  (5169) embryo 06-08hr  (6352) embryo 08-10hr  (5703) embryo 10-12hr  (6246) embryo 12-14hr  (3451) embryo 14-16hr  (5253) embryo 16-18hr  (3629) embryo 18-20hr  (2353) embryo 20-22hr  (3137) embryo 22-24hr  (2427) larva L1  (1783) larva L2  (1368) larva L3 12hr old  (1212) larva L3 puffstage 1-2  (1339) larva L3 puffstage 3-6  (2060) larva L3 puffstage 7-9  (2043) white prepupae new  (2088) white prepupae 12hr  (2475) white prepupae 24hr  (3123) pupae 2d postWPP  (2215) pupae 3d postWPP  (1247) pupae 4d postWPP  (1317) adult male 01day  (1513) adult male 05day  (2045) adult male 30day  (2105) adult female 01day  (2657) adult female 05day  (3015) adult female 30day  (3455) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (7565) embryo 02-04hr  (8875) embryo 04-06hr  (5169) embryo 06-08hr  (6352) embryo 08-10hr  (5703) embryo 10-12hr  (6246) embryo 12-14hr  (3451) embryo 14-16hr  (5253) embryo 16-18hr  (3629) embryo 18-20hr  2353 embryo 20-22hr  (3137) embryo 22-24hr  2427 larva L1  1783 larva L2    1368 larva L3 12hr old    1212 larva L3 puffstage 1-2    1339 larva L3 puffstage 3-6  2060 larva L3 puffstage 7-9  2043 white prepupae new  2088 white prepupae 12hr  (2475) white prepupae 24hr  (3123) pupae 2d postWPP  2215 pupae 3d postWPP    1247 pupae 4d postWPP    1317 adult male 01day  1513 adult male 05day  2045 adult male 30day  2105 adult female 01day  (2657) adult female 05day  (3015) adult female 30day  (3455) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    7565 embryo 02-04hr    8875 embryo 04-06hr    5169 embryo 06-08hr    6352 embryo 08-10hr    5703 embryo 10-12hr    6246 embryo 12-14hr    3451 embryo 14-16hr    5253 embryo 16-18hr    3629 embryo 18-20hr    2353 embryo 20-22hr    3137 embryo 22-24hr    2427 larva L1    1783 larva L2    1368 larva L3 12hr old    1212 larva L3 puffstage 1-2    1339 larva L3 puffstage 3-6    2060 larva L3 puffstage 7-9    2043 white prepupae new    2088 white prepupae 12hr    2475 white prepupae 24hr    3123 pupae 2d postWPP    2215 pupae 3d postWPP    1247 pupae 4d postWPP    1317 adult male 01day    1513 adult male 05day    2045 adult male 30day    2105 adult female 01day    2657 adult female 05day    3015 adult female 30day    3455 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    7565 embryo 02-04hr    8875 embryo 04-06hr    5169 embryo 06-08hr    6352 embryo 08-10hr    5703 embryo 10-12hr    6246 embryo 12-14hr    3451 embryo 14-16hr    5253 embryo 16-18hr    3629 embryo 18-20hr    2353 embryo 20-22hr    3137 embryo 22-24hr    2427 larva L1    1783 larva L2    1368 larva L3 12hr old    1212 larva L3 puffstage 1-2    1339 larva L3 puffstage 3-6    2060 larva L3 puffstage 7-9    2043 white prepupae new    2088 white prepupae 12hr    2475 white prepupae 24hr    3123 pupae 2d postWPP    2215 pupae 3d postWPP    1247 pupae 4d postWPP    1317 adult male 01day    1513 adult male 05day    2045 adult male 30day    2105 adult female 01day    2657 adult female 05day    3015 adult female 30day    3455 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0013764    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0013764 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0013764 expression level Tissue   Expression Level Larval Central Nervous System    350.525 Larval Midgut    117.8 Larval Hindgut    164.9 Larval Malpighian Tubules    158.6 Larval Fat Body    131.5 Larval Salivary Gland    184.5 Larval Trachea    165.125 Larval Carcass    145.125 Adult Head    138.7 Adult Eye    167.55 Adult Brain    218.2 Adult Thoracic-Abdominal Ganglion    195.2 Adult Crop    160.5 Adult Midgut    89.7 Adult Hindgut    126.2 Adult Malpighian Tubules    131.2 Adult Fat Body    113.6 Adult Salivary Gland    235 Adult Heart    97.675 Adult VirginFemale Spermatheca    174 Adult InseminatedFemale Spermatheca    157.8 Adult Ovary    395 Adult Testis    90.8 Adult Male Accessory Gland    183.1 Adult Carcass    100.2 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    350.525 Larval Midgut    117.8 Larval Hindgut    164.9 Larval Malpighian Tubules    158.6 Larval Fat Body    131.5 Larval Salivary Gland    184.5 Larval Trachea    165.125 Larval Carcass    145.125 Adult Head    138.7 Adult Eye    167.55 Adult Brain    218.2 Adult Thoracic-Abdominal Ganglion    195.2 Adult Crop    160.5 Adult Midgut    89.7 Adult Hindgut    126.2 Adult Malpighian Tubules    131.2 Adult Fat Body    113.6 Adult Salivary Gland    235 Adult Heart    97.675 Adult VirginFemale Spermatheca    174 Adult InseminatedFemale Spermatheca    157.8 Adult Ovary    395 Adult Testis    90.8 Adult Male Accessory Gland    183.1 Adult Carcass    100.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    350.525 Larval Midgut    117.8 Larval Hindgut    164.9 Larval Malpighian Tubules    158.6 Larval Fat Body    131.5 Larval Salivary Gland    184.5 Larval Trachea    165.125 Larval Carcass    145.125 Adult Head    138.7 Adult Eye    167.55 Adult Brain    218.2 Adult Thoracic-Abdominal Ganglion    195.2 Adult Crop    160.5 Adult Midgut    89.7 Adult Hindgut    126.2 Adult Malpighian Tubules    131.2 Adult Fat Body    113.6 Adult Salivary Gland    235 Adult Heart    97.675 Adult VirginFemale Spermatheca    174 Adult InseminatedFemale Spermatheca    157.8 Adult Ovary    395 Adult Testis    90.8 Adult Male Accessory Gland    183.1 Adult Carcass    100.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    350.525 Larval Midgut    117.8 Larval Hindgut    164.9 Larval Malpighian Tubules    158.6 Larval Fat Body    131.5 Larval Salivary Gland    184.5 Larval Trachea    165.125 Larval Carcass    145.125 Adult Head    138.7 Adult Eye    167.55 Adult Brain    218.2 Adult Thoracic-Abdominal Ganglion    195.2 Adult Crop    160.5 Adult Midgut    89.7 Adult Hindgut    126.2 Adult Malpighian Tubules    131.2 Adult Fat Body    113.6 Adult Salivary Gland    235 Adult Heart    97.675 Adult VirginFemale Spermatheca    174 Adult InseminatedFemale Spermatheca    157.8 Adult Ovary    395 Adult Testis    90.8 Adult Male Accessory Gland    183.1 Adult Carcass    100.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0013764 expression level Tissue   Expression Level Larval Central Nervous System    350.525 Larval Midgut    117.8 Larval Hindgut    164.9 Larval Malpighian Tubules    158.6 Larval Fat Body    131.5 Larval Salivary Gland    184.5 Larval Trachea    165.125 Larval Carcass    145.125 Adult Head    138.7 Adult Eye    167.55 Adult Brain    218.2 Adult Thoracic-Abdominal Ganglion    195.2 Adult Crop    160.5 Adult Midgut    89.7 Adult Hindgut    126.2 Adult Malpighian Tubules    131.2 Adult Fat Body    113.6 Adult Salivary Gland    235 Adult Heart    97.675 Adult VirginFemale Spermatheca    174 Adult InseminatedFemale Spermatheca    157.8 Adult Ovary    395 Adult Testis    90.8 Adult Male Accessory Gland    183.1 Adult Carcass    100.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    350.525 Larval Midgut    117.8 Larval Hindgut    164.9 Larval Malpighian Tubules    158.6 Larval Fat Body    131.5 Larval Salivary Gland    184.5 Larval Trachea    165.125 Larval Carcass    145.125 Adult Head    138.7 Adult Eye    167.55 Adult Brain    218.2 Adult Thoracic-Abdominal Ganglion    195.2 Adult Crop    160.5 Adult Midgut    89.7 Adult Hindgut    126.2 Adult Malpighian Tubules    131.2 Adult Fat Body    113.6 Adult Salivary Gland    235 Adult Heart    97.675 Adult VirginFemale Spermatheca    174 Adult InseminatedFemale Spermatheca    157.8 Adult Ovary    395 Adult Testis    90.8 Adult Male Accessory Gland    183.1 Adult Carcass    100.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    350.525 Larval Midgut    117.8 Larval Hindgut    164.9 Larval Malpighian Tubules    158.6 Larval Fat Body    131.5 Larval Salivary Gland    184.5 Larval Trachea    165.125 Larval Carcass    145.125 Adult Head    138.7 Adult Eye    167.55 Adult Brain    218.2 Adult Thoracic-Abdominal Ganglion    195.2 Adult Crop    160.5 Adult Midgut    89.7 Adult Hindgut    126.2 Adult Malpighian Tubules    131.2 Adult Fat Body    113.6 Adult Salivary Gland    235 Adult Heart    97.675 Adult VirginFemale Spermatheca    174 Adult InseminatedFemale Spermatheca    157.8 Adult Ovary    395 Adult Testis    90.8 Adult Male Accessory Gland    183.1 Adult Carcass    100.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    350.525 Larval Midgut    117.8 Larval Hindgut    164.9 Larval Malpighian Tubules    158.6 Larval Fat Body    131.5 Larval Salivary Gland    184.5 Larval Trachea    165.125 Larval Carcass    145.125 Adult Head    138.7 Adult Eye    167.55 Adult Brain    218.2 Adult Thoracic-Abdominal Ganglion    195.2 Adult Crop    160.5 Adult Midgut    89.7 Adult Hindgut    126.2 Adult Malpighian Tubules    131.2 Adult Fat Body    113.6 Adult Salivary Gland    235 Adult Heart    97.675 Adult VirginFemale Spermatheca    174 Adult InseminatedFemale Spermatheca    157.8 Adult Ovary    395 Adult Testis    90.8 Adult Male Accessory Gland    183.1 Adult Carcass    100.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    138.7   NA  Eye    167.55   NA  Brain    218.2   350.525  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    195.2   NA  Crop    160.5   117.8  Midgut    89.7   164.9  Hindgut    126.2   158.6  Malpighian Tubules    131.2   131.5  Fat Body    113.6   184.5  Salivary Gland    235   NA  Heart    97.675   165.125  Trachea    NA   NA  VirginFemale Spermatheca    174   NA  InseminatedFemale Spermatheca    157.8   NA  Ovary    395   NA  Testis    90.8   NA  Male Accessory Gland    183.1   145.125  Carcass    100.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013764 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3924-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | recessive Chig96.1 lethal | larval stage Chik04405 lethal | larval stage | recessive Chie5.5 Chig1 lethal | non-rescuable maternal effect | recessive Chie5.5 lethal | recessive Chik04405 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 ChiGD4080 viable ChiE ChiEY05956 Sterility Allele fertile ChiEY05956 Other Phenotypes Allele eclosion defective, with Scer\GAL4Ccap.PP ChiΔDD.Scer\UAS.T:Hsap\MYC ChiΔLID.Scer\UAS.T:Hsap\MYC eclosion defective | heat sensitive, with Scer\GAL4Ccap.PP, Scer\GAL80ts.αTub84B ChiΔLID.Scer\UAS.T:Hsap\MYC neuroanatomy defective | recessive Chie5.5 visible ChiE visible, with Scer\GAL4ap-md544 ChiFL.Scer\UAS.T:Hsap\MYC ChiScer\UAS.cMa ChiΔDD.Scer\UAS.T:Hsap\MYC ChiΔLID.Scer\UAS.T:Hsap\MYC ChiΔLID.Scer\UAS visible, with Scer\GAL4pnr-MD237 ChiGD4080 visible | dominant Chie5.5 ChiE ChiM3 ChiM4 visible | recessive ChiE visible | recessive | somatic clone Chie5.5 wild-type Chi+t7.4 Phenotype manifest in Allele adult antennal lobe projection neuron Chie5.5 adult thorax ChiE alula, with Scer\GAL4ap-md544 ChiΔLID.Scer\UAS antennal lobe glomerulus D Chie5.5 antennal lobe glomerulus DA1 Chie5.5 antennal lobe glomerulus DL1 Chie5.5 antennal lobe glomerulus DM1 Chie5.5 antennal lobe glomerulus DM2 Chie5.5 antennal lobe glomerulus DM5 Chie5.5 antennal lobe glomerulus DM6 Chie5.5 antennal lobe glomerulus VA1 Chie5.5 antennal lobe glomerulus VA1 lateral compartment Chie5.5 antennal lobe glomerulus VA1 medial compartment Chie5.5 antennal lobe glomerulus VA3 Chie5.5 antennal lobe glomerulus VA5 Chie5.5 antennal lobe glomerulus VM2 Chie5.5 antennal lobe glomerulus VM7 Chie5.5 antennal lobe projection neuron adPN Chie5.5 chaeta | supernumerary, with Scer\GAL4pnr-MD237 ChiGD4080 dorsocentral bristle ChiE dorsocentral bristle | supernumerary Chie5.5 embryo Chig96.1 embryo | germline clone Chie5.5 embryonic/first instar larval cuticle | germline clone Chie5.5 embryonic/larval neuron Chie5.5 interneuron | embryonic stage Chie5.5 leg | somatic clone Chie5.5 mesothoracic tergum, with Scer\GAL4ap-md544 ChiΔSAD.Scer\UAS mesothoracic tergum, with Scer\GAL4pnr-MD237 ChiGD4080 posterior postalar bristle ChiE pretarsus, with Scer\GAL4Dll-md23 ChiΔLID.Scer\UAS.T:Hsap\MYC ChiFL.Scer\UAS.T:Hsap\MYC pretarsus | somatic clone Chie5.5 scutellar bristle | ectopic ChiE scutellar bristle | supernumerary ChiE Chie5.5 subesophageal ganglion Chie5.5 tarsal segment 4, with Scer\GAL4Dll-md23 ChiΔLID.Scer\UAS.T:Hsap\MYC ChiFL.Scer\UAS.T:Hsap\MYC tarsal segment 4 | somatic clone Chie5.5 thorax ChiE thorax | somatic clone ChiErv1 trichogen cell, with Scer\GAL4pnr-MD237 ChiGD4080 unguis, with Scer\GAL4Dll-md23 ChiΔLID.Scer\UAS.T:Hsap\MYC ChiFL.Scer\UAS.T:Hsap\MYC unguis | somatic clone Chie5.5 wing ChiE ChiM3 ChiM4 wing, with Scer\GAL4ap-md544 ChiΔDD.Scer\UAS.T:Hsap\MYC ChiΔLCCD.Scer\UAS.T:Hsap\MYC ChiΔLID.R.Scer\UAS ChiΔLID.Scer\UAS.T:Hsap\MYC ChiΔSAD.Scer\UAS ChiFL.Scer\UAS.T:Hsap\MYC ChiScer\UAS.T:Hsap\MYC ChiScer\UAS.cFa ChiScer\UAS.cMa wing, with Scer\GAL4Ccap.PP ChiΔDD.Scer\UAS.T:Hsap\MYC ChiΔLID.Scer\UAS.T:Hsap\MYC wing, with Scer\GAL4dpp.blk1 ChiΔLID.R.Scer\UAS ChiΔSAD.Scer\UAS wing | distal, with Scer\GAL4ap-md544 ChiΔLID.Scer\UAS wing | dorsal/ventral compartment boundary ChiM4 wing | dorsal/ventral compartment boundary, with Scer\GAL4dpp.blk1 ChiScer\UAS.cFa wing | dorsal compartment | somatic clone Chie5.5 wing | heat sensitive, with Scer\GAL4Ccap.PP, Scer\GAL80ts.αTub84B ChiΔLID.Scer\UAS.T:Hsap\MYC wing | somatic clone Chie5.5 wing disc | somatic clone Chie5.5 wing margin | distal, with Scer\GAL4dpp.blk1 ChiΔLID.R.Scer\UAS wing margin | ectopic ChiM4 wing margin | ectopic, with Scer\GAL4dpp.blk1 ChiΔSAD.Scer\UAS ChiScer\UAS.cFa wing margin | ectopic | somatic clone Chie5.5 wing margin | somatic clone Chie5.5
Classical Alleles ( 14 )
For All Classical Alleles Show
Allele of Chi Class Mutagen Stocks Known lesion Chie5.5 amorphic allele - genetic evidence ethyl methanesulfonate 3 Yes Chic03428 1 -- Chid02478 P-element activity 1 -- ChiE 1 Yes ChiEY05956 P-element activity 1 -- ChiErv1 ethyl methanesulfonate 0 Yes Chig1 gamma ray 0 Yes Chig371 0 Yes Chig96.1 antimorphic allele - genetic evidence gamma ray 0 -- Chik04405 P-element activity 0 Yes ChiM3 ethyl methanesulfonate 0 Yes ChiM4 ethyl methanesulfonate 0 -- ChiMAN1-Δ6 Delta2-3 0 Yes Chiunspecified 0 --
Alleles Carried on Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Chi Class Mutagen Stocks Known lesion ChiGD4080 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes ChiGL00360 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ChiJF01497 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ChiKK108451 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Chi+t7.4 in vitro construct - genomic fragment 0 Yes ChiE.Act5C.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes ChiFL.Act5C.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes ChiFL.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes ChiScer\UAS.cFa in vitro construct - regulatory fusion 0 Yes ChiScer\UAS.cMa in vitro construct - regulatory fusion 0 Yes ChiScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion 0 Yes ChiT:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes ChitCH322-91I05 in vitro construct - genomic fragment 0 Yes ChiΔDD.Act5C.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes ChiΔDD.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes ChiΔLCCD.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes ChiΔLID.Act5C.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes ChiΔLID.R.Scer\UAS in vitro construct - deletion 0 Yes ChiΔLID.Scer\UAS.T:Hsap\MYC in vitro construct - deletion 0 Yes ChiΔLID.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes ChiΔOID.Act5C.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes ChiΔSAD.Scer\UAS in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC136 (Christensen and Cook, 2006.1.19) Df(2R)Chig230 (Morcillo et al., 1997) Df(2R)Exel6080 (Ryder, 2004.4.26) Df(2R)MAN1Δ26 (Pinto et al., 2008) Df(2R)or-BR11 (Ramain et al., 2000, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2R)bwVDe2LPxKR (BDGP Project Members, 1994-1999, Beaton, 1997.12.27) Df(2R)bw-S46 (BDGP Project Members, 1994-1999) Df(2R)MAN1Δ47 (Pinto et al., 2008) Df(2R)MAN1Δ128 (Pinto et al., 2008) Df(2R)MAN1Δ296 (Pinto et al., 2008) In(2LR)ltG10LBR27R (BDGP Project Members, 1994-1999) In(2LR)Px4 (BDGP Project Members, 1994-1999)
Partially disrupted in	Df(2R)MAN1Δ6 (Pinto et al., 2008)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4080} NA P{KK108451} NA P{TRiP.GL00360} NA P{TRiP.JF01497} NA P{UAS-Chi.FL} NA P{UAS-Chi.M} NA P{UAS-Chi.Δ387-426} NA P{UAS-Chi.ΔDD} NA P{UAS-Chi.ΔLID.M} NA P{UAS-Chi.ΔLID} NA P{UAS-Chip.F} NA P{UAS-Chip.MYC} NA P{UAS-Chip.ΔLID.R} NA P{UAS-Chip.ΔSAD} NA characterization construct P{Chi+7.4} NA P{UAS-ap::Chi.M} NA P{UAS-ChiΔLID::apΔLIM} NA PBac{CH322-91I05} NA PBac{Chi-EGFP.S} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}ChiEY05956 NA P{XP}Chid02478 NA miscellaneous insertions P{GD4080}Chiv30454 NA PBac{PB}Chic03428 NA insertion of enhancer trap P{lacW}Chik04405 No
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Iswi (Vanolst et al., 2005) inferred from physical interaction with tou (Vanolst et al., 2005) RNA polymerase II transcription factor binding inferred from physical interaction with tup (Biryukova and Heitzler, 2005) transcription factor binding inferred from physical interaction with pnr (Vanolst et al., 2005)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (van Meyel et al., 2000) chaeta development inferred from mutant phenotype (de Navascués and Modolell, 2010) chaeta morphogenesis inferred from mutant phenotype (Biryukova and Heitzler, 2005) dendrite guidance inferred from mutant phenotype (Komiyama and Luo, 2007) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Rincon-Limas et al., 2000) nervous system development inferred from mutant phenotype (van Meyel et al., 2000) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) positive regulation of transcription, DNA-dependent inferred from mutant phenotype (Kojima et al., 2005) regulation of eclosion inferred from mutant phenotype (Hari et al., 2008) transcription, DNA-dependent inferred from mutant phenotype (van Meyel et al., 2000)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (van Meyel et al., 2000)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
transcription cofactor activity inferred from electronic annotation with InterPro:IPR002691 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
blastoderm segmentation traceable author statement (van Meyel et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Chi allele Gene References Chie5.5 ap (Morcillo et al., 1997, Shoresh et al., 1998) ap::Chi (van Meyel et al., 2000) Bx (Shoresh et al., 1998, Milan et al., 1998) caps (Milan et al., 2001) ct (Morcillo et al., 1996) N (Rollins et al., 1999) Nipped-A (Rollins et al., 1999) RpL38 (Rollins et al., 1999) Ser (Milan and Cohen, 2000) Ssdp (Chen et al., 2002) trn (Milan et al., 2001) ChiE brm (Heitzler et al., 2003) osa (Heitzler et al., 2003) ChiFL.Scer\UAS.T:Hsap\MYC ap (van Meyel et al., 1999) B (Pueyo and Couso, 2004) Bx (Pueyo and Couso, 2004) Ssdp (van Meyel et al., 2003) Chig1 ct (Morcillo et al., 1996) Chig96.1 bcd (Torigoi et al., 2000) ct (Morcillo et al., 1996) Chik04405 ct (Morcillo and Dorsett, 1996) Ubx (Morcillo and Dorsett, 1996) ChiM4 ap (Fernandez-Funez et al., 1998) ChiScer\UAS.cFa ap (Fernandez-Funez et al., 1998) ChiScer\UAS.cMa ap (Milan and Cohen, 1999) ChiScer\UAS.T:Hsap\MYC ap (Rincon-Limas et al., 2000) ChiΔDD.Scer\UAS.T:Hsap\MYC ap (van Meyel et al., 1999) ChiΔLID.Scer\UAS ap::Chi (Milan and Cohen, 1999) Bx (Milan and Cohen, 1999) unspecified ap (Lu et al., 1998, Milan et al., 2001) Bx (Milan et al., 2001) ct (Milan et al., 2001) mam (Milan et al., 2001) mor (Heitzler et al., 2003) osa (Milan et al., 2001) Ser (Milan et al., 2001) su(Hw) (Morcillo et al., 1996) tup (Biryukova and Heitzler, 2005) vg (Milan et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63418 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0013764 DroID - A comprehensive database of gene and protein interactions. • FBgn0013764 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37837
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12038 Dere\GG19962 Dgri\GH21714 Dmoj\GI18855 Dper\GL10430 Dpse\GA17778 Dsec\GM18282 Dsim\GD24979 Dvir\GJ20439 Dwil\GK21383 Dyak\GE11494
Curated drosophilid orthologs	Dpse\GA17778
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL011058-PA Aedes aegypti (mosquito) AAEL013094-PA Aedes aegypti (mosquito) AGAP004691-PA Anopheles gambiae (mosquito) XP_394712.3 Apis mellifera (honey bee) XP_001946705.1 Acyrthosiphon pisum (pea aphid) BGIBMGA012202-PA Bombyx mori (silk worm) ENSBTAP00000007599 Bos taurus (bovine) CN12261 Caenorhabditis brenneri (small nematode) CBP26987 Caenorhabditis briggsae (nematode) CE23789 Caenorhabditis elegans (worm) ENSCAFP00000014734 Canis familiaris (dog) ENSCINP00000008452 Ciona intestinalis (sea squirt) JA12539 Caenorhabditis japonica (nematode) CPIJ004474 Culex pipens (mosquito) ENSCPOP00000009260 Cavia porcellus (guinea pig) RP38004 Caenorhabditis remanei (nematode) ENSCSAVP00000000584 Ciona savignyi (sedentary tunicate) 222123 Capitella spl (polychaete worm) 42148 Daphnia pulex (daphnia) ENSDARP00000092375 Danio rerio (zebra fish) ENSECAP00000006672 Equus caballus (horse) ENSGACP00000024241 Gymnopilus aculeatus (mushroom) ENSGALP00000038620 Gallus gallus (chicken) 188929 Hypsipyla robusta (cedar shoot borer) ENSP00000306772 Homo sapiens (human) ISCW010935-PA Ixodes scapularis (deer tick) 184392 Lottia gigantea (owl limpet) ENSMODP00000001085 Monodelphis domestica (gray opossum) ENSMMUP00000012277 Macaca mulatta (rhesus monkey) ENSMUSP00000026252 Mus musculus (house mouse) ENSMUSP00000067737 Mus musculus (house mouse) 103860 Nematostella vectensis (starlet anemone) XP_001599552.1 Nasonia vitripennis (wasp) ENSOANP00000012472 Ornithorynchus anatinus (platypus) ENSORLP00000015489 Oryzias latipes (japanese killifish) PHUM008899-PA Pediculus humanus (human body louse) PP00367 Pristionchus pacificus (nematode) 171180 Physcomitrella patens (moss) ENSPPYP00000016337 Pongo pygmaeus (orangutan) ENSPTRP00000027443 Pan troglodytes (chimpanzee) ENSRNOP00000055594 Rattus norvegicus (norway rat) 5039876 Sorghum bicolor (sorghum) XP_001185744.1 Strongylocentrotus purpuratus (purple sea urchin) XP_969024.1 Tribolium castaneum (red flour beetle) ENSTNIP00000006607 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000028515 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000003595 Xenopus tropicalis (frog) YALI0C05060g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18520 LH17755 ISCW010935 FBgn0229298 FBgn0223375 FBgn0207579 FBgn0196292 FBgn0173189 JGI_V11_42148 FBgn0077787 FBgn0148040 FBgn0141594 FBgn0129175 FBgn0112162 FBgn0096029 FBgn0089077 CPIJ004474 CPIJ011950 BGIBMGA012202 ACYPI001416 GB30239 GB14041 AGAP004691 AAEL013094 AAEL011058 AAEL004737 PB25237 PHUM362400 GLEAN_00244
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Bloomington	36527 w1118; ChiE 31049 y1 v1; P{TRiP.JF01497}attP2 16378 y1 w67c23; P{EPgy2}ChiEY05956 35435 y1 sc* v1; P{TRiP.GL00360}attP2 4541 y1 w*; Chie5.5/CyO-Df(2R)B80, y+ 36500 y1 w*; P{FRT(whs)}G13 P{GawB}GH146 P{UAS-mCD8::GFP.L}LL5 Chie5.5/CyO
Harvard	- P{XP}Chid02478 - PBac{PB}Chic03428
Kyoto	107789 y1 w*; Chie5.5/CyO, Ts(Y;2Lt)B80, Kr- y+
VDRC	v107314 P{KK108451}VIE-260B v43934 w1118; P{GD4080}v43934 v30454 w1118; P{GD4080}Chiv30454
Genomic Clones ( 1 )
	BACR28M05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 229 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD25129 RE67266
Other clones	DPiM_LD25129
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT01557 AT24710 GH01158 GH25856 HL05955 LD01523 LD03104 LD09839 LD10332 LD12003 LD13510 LD13916 LD15379 LD18075 LD21566 LD25062 LD25238 LD26006 LD26102 LD33471 LD34877 LD34878 LD36487 LD36488 LD36937 LD38654 LD38686 LD40778 LD41249 LD42549 LD44877 LD46669 LD46694 LD46737 LD46953 LP03567 LP14129 RE03675 RE05768 RE20875 RE28192 RE32992 RE33171 RE47435 RH46113 SD01333 SD05856 SD08984 SD09664 SD20469
Other clones	461 15715 30275 30565 61638 76199 107576 183130 214467.69 Dmel_dig_001824_0680_0309 Dmel_neb_011763_0846_3456 Dmel_neb_013308_0883_2037 EK122029 EK139621 EK139757 EK187239 EK205604 EK276506 EK288051 EP15743 FGM211F01 IP03877 IP03977 IP04062 RP001024227 RP003003451 RP003020319 RP003022714 RP003024098 RP003028143 RP003030640 RP003036533 RP003039384 RP003043101 RP003045146 RP003059626 RP003077216 RP003078563 RP003079661 RP003087191 RP003087395 RP003089699 RP003098570 RP003101231 RP003108341 RP003119015 RP003124591 RP003126049 RP003127850 RP003128308 RP003130548 RP003131065 RP003144937 RP003146504 RP003146695 RP003148666 RP003149328 RP003151925 RP003156505 RP003160011 RP003161571 RP003162452 RP003163260 RP003168602 RP003173577 RP003187938 RP003192494 RP003213713 RP003214473 RP003217963 RP003224824 RP003229093 RP003236411 RP003245839 RP003249403 RP003251303 RP003252036 RP003256522 RP003262744 RP003263007 RP003265039 RP003265397 RP003266427 RP003267534 RP003269434 RP003270588 RP003271146 RP003273729 RP003282224 RP003283607 RP003287462 RP003287473 RP003295065 RP003295607 RP003298482 RP003298981 RP003303239 RP003317041 RP003321917 RP003326006 RP003334678 RP003334954 RP003338770 RP003351991 RP003352592 RP003352731 RP003354503 RP003354586 RP003356149 RP003358370 RP003364361 RP003367653 RP003367923 RP003368473 RP003371322 RP003373933 RP003374686 RP003386241 RP003410860 RP003422435 RP003424339 RP003436342 RP003452705 RP003457262 RP003481203 RP003482113 RP003483761 RP003486754 RP003490682 RP003491446 RP003492832 RP003498794 RP003502921 RP003508631 RP003511113 RP003521643 RP003524103 RP003525188 RP003529072 RP003531446 RP003544339 RP003561049 RP003564998 RP003565348 RP003566741 RP003569458 RP003578602 RP003584257 RP003584402 RP003588241 RP003590341 RP003593216 RP003594910 RP003595618 RP003598639 RP003616480 RP003618054 RP003621296 RP003627971 RP003630553 RP003630925 RP003638319 RP003643593 RP003646969 RP003649561 RP003650422 RP003655638 RP003665176 RP003665917 RP003668592 RP003673341 RP003673755 RP003676463 RP003678616 SD12770 SD23064
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013764 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37837
Antibody Information
	polyclonal (Morcillo et al., 1997)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Chi CG3924 (Wang, 1999.11)
Source for database merge of	Source for merge of: Chi Ldb (Fernandez-Funez et al., 1998)
Additional comments	"Ldb" may correspond to "Chi". (Botas, 1998.3.13)
Other Comments
	Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB. (Liu and Lehmann, 2008) S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) tou, pnr and Chi co-operate during neural development. (Vanolst et al., 2005) Ssdp interacts with Chi to regulate development. (Chen et al., 2002) The ap:Chi complex may act as a tetramer. (Rincon-Limas et al., 2000) Chi has a role in segmentation. (Torigoi et al., 2000) Chi is an essential cofactor for ap in the regulation of axon guidance. (van Meyel et al., 2000) ap activity depends on the formation of a LIM homeodomain dimer bridged by a dimer of Chi cofactor. (Milan and Cohen, 1999) Chi has roles in wing development beyond mediating ap activity and regulating ct. (Rollins et al., 1999) Chi acts as a cofactor for ap in wing development. (van Meyel et al., 1999) Lack and excess of Chi gene product cause the same phenotype as lack of ap function: dorsal to ventral transformations, generation of new wing margins, wing outgrowths. (Fernandez-Funez et al., 1998) The normal function of ap in dorso-ventral compartmentalization in the wing requires the correct proportion of Chi and ap gene product. (Fernandez-Funez et al., 1998) Chi is identified as a ubiquitously expressed gene whose protein product interacts with ap LIM domains in the yeast two-hybrid assay. (Lu et al., 1998) Mutations in Chi show strong genetic interaction with ap. In spite of its generalized expression, lack of Chi function in genetic mosaics produces specific phenotypes identical to the phenotype of ap mutant clones; i.e., dorsoventral transformation, generation of new wing margins and wing outgrowths. Overexpression of Chi produces a new wing margin, but only where ap is expressed. (Lu et al., 1998) Gene isolated in a screen of the second chromosome identifying mutants affecting disc morphology. (Roch et al., 1998) In analogy to their vertebrate counterparts, the gene products of Bx, ap and Chi form a DNA-binding complex, that regulates wing development. (Shoresh et al., 1998) Chi cooperates with different LIM domain proteins and other factors to structurally support enhancer-promoter interactions. (Morcillo et al., 1997) Chi, but not mam, modulates expression of a gypsy insertion into Ubx. Chi and mam regulate ct alleles lacking gypsy insertions. sd, mam and Chi cooperate synergistically to regulate ct expression. (Morcillo et al., 1996) Genetic analysis suggests that Chi is involved in facilitating long-distance communication between the wing margin enhancer and the promoter of ct. (Morcillo and Dorsett, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057734 NM_166650 RefSeq (Proteins) • NP_477082 NP_726403 DDBJ / EMBL / GenBank • AA941151 AAB62574 AAF47142 AAL13790 AAM68293 AC004642 AF010325 AF010326 AF010327 AF010328 AW942718 AY058561 CL527848 CZ472844 Entrez Gene - A searchable database of RefSeq genes. • 37837 UniProtKB/TrEMBL • O18353 Q7KVG9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • LIM binding protein (IPR002691)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63418 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0013764 DroID - A comprehensive database of gene and protein interactions. • FBgn0013764 DRSC - Results from RNAi screens. • FBgn0013764 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013764 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3924-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013764 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37837 InParanoid A subset of ortholog calls from InParanoid. • AAEL011058-PA Aedes aegypti (mosquito) AAEL013094-PA Aedes aegypti (mosquito) AGAP004691-PA Anopheles gambiae (mosquito) XP_394712.3 Apis mellifera (honey bee) XP_001946705.1 Acyrthosiphon pisum (pea aphid) BGIBMGA012202-PA Bombyx mori (silk worm) ENSBTAP00000007599 Bos taurus (bovine) CN12261 Caenorhabditis brenneri (small nematode) CBP26987 Caenorhabditis briggsae (nematode) CE23789 Caenorhabditis elegans (worm) ENSCAFP00000014734 Canis familiaris (dog) ENSCINP00000008452 Ciona intestinalis (sea squirt) JA12539 Caenorhabditis japonica (nematode) CPIJ004474 Culex pipens (mosquito) ENSCPOP00000009260 Cavia porcellus (guinea pig) RP38004 Caenorhabditis remanei (nematode) ENSCSAVP00000000584 Ciona savignyi (sedentary tunicate) 222123 Capitella spl (polychaete worm) 42148 Daphnia pulex (daphnia) ENSDARP00000092375 Danio rerio (zebra fish) ENSECAP00000006672 Equus caballus (horse) ENSGACP00000024241 Gymnopilus aculeatus (mushroom) ENSGALP00000038620 Gallus gallus (chicken) 188929 Hypsipyla robusta (cedar shoot borer) ENSP00000306772 Homo sapiens (human) ISCW010935-PA Ixodes scapularis (deer tick) 184392 Lottia gigantea (owl limpet) ENSMODP00000001085 Monodelphis domestica (gray opossum) ENSMMUP00000012277 Macaca mulatta (rhesus monkey) ENSMUSP00000026252 Mus musculus (house mouse) ENSMUSP00000067737 Mus musculus (house mouse) 103860 Nematostella vectensis (starlet anemone) XP_001599552.1 Nasonia vitripennis (wasp) ENSOANP00000012472 Ornithorynchus anatinus (platypus) ENSORLP00000015489 Oryzias latipes (japanese killifish) PHUM008899-PA Pediculus humanus (human body louse) PP00367 Pristionchus pacificus (nematode) 171180 Physcomitrella patens (moss) ENSPPYP00000016337 Pongo pygmaeus (orangutan) ENSPTRP00000027443 Pan troglodytes (chimpanzee) ENSRNOP00000055594 Rattus norvegicus (norway rat) 5039876 Sorghum bicolor (sorghum) XP_001185744.1 Strongylocentrotus purpuratus (purple sea urchin) XP_969024.1 Tribolium castaneum (red flour beetle) ENSTNIP00000006607 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000028515 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000003595 Xenopus tropicalis (frog) YALI0C05060g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/chip1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37837 modMine - Data generated by the modENCODE project. • FBgn0013764 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18520 LH17755 ISCW010935 FBgn0229298 FBgn0223375 FBgn0207579 FBgn0196292 FBgn0173189 JGI_V11_42148 FBgn0077787 FBgn0148040 FBgn0141594 FBgn0129175 FBgn0112162 FBgn0096029 FBgn0089077 CPIJ004474 CPIJ011950 BGIBMGA012202 ACYPI001416 GB30239 GB14041 AGAP004691 AAEL013094 AAEL011058 AAEL004737 PB25237 PHUM362400 GLEAN_00244
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	CG3924 (Keleman et al., 2009.8.5, Gandhi et al., 2007, Ni et al., 2008, Ni et al., 2011.7.19) Chi (Stroschein-Stevenson et al., 2006, Venken et al., 2009, Liu and Lehmann, 2008, Venken et al., 2009, Beltran et al., 2007, Wasbrough et al., 2010) Chip (Zenvirt et al., 2008, Roignant et al., 2007, Heitzler, 2006.8.9, Roignant et al., 2010, Delanoue et al., 2002, de Navascués and Modolell, 2007, Pinto et al., 2008, Komiyama and Luo, 2007, Asmar et al., 2008) chip (Luschnig et al., 2004, Milan et al., 1998, Hari et al., 2008) CHIP (Bronstein et al., 2010) dLdb (Jurata et al., 2000, Lu et al., 1998) dLDB/Chip (Weihe et al., 2001) l(2)04405 (Kolhekar et al., 1997) l(2)k04405   Ldb (Botas, 1998.3.13, ) LDB (Bronstein et al., 2010)
Name Synonym	Chip (Milan et al., 2001, Bell et al., 2001, Milan and Cohen, 2000, van Meyel et al., 2000, Morcillo et al., 2000, Sun and Elgin, 1999, Morcillo, 1997.6.19, Morcillo, 1997.6.19, Morcillo, 1997.6.19, Morcillo, 1997.6.19, van Meyel et al., 1999, Milan and Cohen, 1999, Rollins et al., 1999, Shoresh et al., 1998, Curtiss and Heilig, 1998, Geyer, 1997, Morcillo and Dorsett, 1995, Biryukova and Heitzler, 2005, Roignant et al., 2010, Asmar et al., 2007, Biryukova and Heitzler, 2008, Hari et al., 2008, de Navascués and Modolell, 2010)
Secondary FlyBase IDs
	FBgn0020536 FBgn0024338
References ( 111 )
Generate a list of	All references relating to this gene ( 111 )
List References by type	Research paper  ( 47 ) Supplementary material  ( 7 ) Review  ( 18 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 16 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 4 ) Curated genome annotation  ( 1 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 )
Recent research papers ( 4 )
	Gabilondo et al., 2011, Mech. Dev. 128(3-4): 208--221 A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons. [FBrf0213290] Bronstein et al., 2010, PLoS Genet. 6(8): e1001063 Transcriptional regulation by CHIP/LDB complexes. [FBrf0211594] de Navascués and Modolell, 2010, Mech. Dev. 127(9-12): 393--406 The pronotum LIM-HD gene tailup is both a positive and a negative regulator of the proneural genes achaete and scute of Drosophila. [FBrf0212072] Roignant et al., 2010, Development 137(2): 273--281 The transcriptional co-factor Chip acts with LIM-homeodomain proteins to set the boundary of the eye field in Drosophila. [FBrf0209594] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...