oblivious, mat(2)syn-C, obv
Gene model reviewed during 5.41
Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cnn using the Feature Mapper tool.
cnn transcripts are first detected at stage 5. High levels are present in gastrulating embryos along the ventral and cephalic furrows and later in the mesoderm. By stage 10, expression is seen in neuroblasts of the CNS, and by stage 11 in the PNS. By stage 14, expression is stronger in the thoracic part of the nerve cord than in the abdominal region and can be seen in the brain hemispheres as well. From stage 11-16, expression is observed in two domains of the visceral mesoderm surrounding the midgut. Double labeling shows that the anterior domain is located just anterior to cells expressing Scr and the posterior domain overlaps with cells expressing Antp and Ubx.
cnn protein localizes to the centrosomes of the first zygotic nucleus. This centrosomal association persists throughout the 13 synchronous rounds of nuclear divisions of the syncytial embryo. During the syncytial divisions, cnn protein is found at all microtubule-organizing centers. The centrosome localization is not dependent on the presence of an intact microtubule cytoskeleton.
cnn protein is expressed at high levels during both mitotic and meiotic divisions. It is closely associated with the centrosomes in a cell-dependent manner throughout spermatogenesis. During spermatogonial cell mitoses, cnn protein is strongly detected at spindle poles. At interphases it is present in centrosomes at much lower levels and is found at high levels in the cytoplasm. During the rapid growth phase of primary spermatocytes, cnn protein is in close association with the centriole. In each spermatocyte, two dots of cnn staining are seen at the membrane, representing the duplicated centrosomes. Later, as cells approach the meiotic divisions, the centrosomes migrate back to the nuclear membranes and higher levels of cnn protein staining are seen in the centrosomes. Accumulation of FBgn0013765:cnn protein in the spermatocyte centrosomes is not dependent on the presence of microtubules. FBgn0013765:cnn protein is detected in the centrosomes during both meiotic divisions. During meiosis II, there is a single centriole in each centrosome. In postmeiotic cells, the single centriole inserts into the nucleus and becomes the basal body. FBgn0013765:cnn @ protein is detected in the basal body until the early stages of axonemal elongation.
The pattern of cnn protein distribution is essentially identical to that of cnn transcripts. Immunostaining demonstrates that there are more cells toward the ventral midline in the thoracic neuromeres that express cnn protein compared to the abdominal neuromeres. Furthermore, cnn protein and transcript expression patterns are not identical in the visceral mesoderm. cnn protein is not visible in the visceral mesoderm at late germ band retraction but is observed in stages 14-16 in the visceral mesoderm of the gastric caecae and the second midgut constriction.
GBrowse - Visual display of RNA-Seq signalsView Dmel\cnn in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of cnn CG18370 was sequence comparison ( date:001104 ).
Gene expression is increased in response to the presence of either one or two copies of Scer\GAL4hs.PB.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes decreased γ-tubulin staining at the spindle pole when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
SL2 cells treated with dsRNA against cnn do not show a significant difference in the mitotic index compared to control cells. The treated cells show anastral bipolar spindle arrangements, with anastral poles that are often barrel-shaped and unfocused, however in spite of these abnormalities, anaphase progresses normally in these cells without and increase in abnormal chromosome arrangement compared to control cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
cnn is required for the assembly and function of centrosomes during the syncytial cleavage divisions in the embryo.
cnn is an essential core component of early embryonic centrosomes.
cnn is required for spindle organisation during male meiosis and for organisation of the sperm axoneme.
Maternal effect mutations at the cnn locus prevent the correct restructuring of cortical microfilaments in the early embryo.
cnn function is required for synchronous nuclear divisions in the early embryo, and for various morphological processes in the female and male germlines.
Protein product associated with centrosomes during blastoderm cell cycles and then with centrosomes of mitotic cells.
cnn is an essential component of the centrosome, it is localised to the centrosome independent of microtubules. Postblastoderm stage cnn localisation and localisation during spermatogenesis and oogenesis is cell-cycle-dependent.
Identified by immunopurification as a target gene for Antp. cnn encodes a centrosomal protein, may be involved in the assembly of mitotic spindles and maps to a genomic region required for midgut morphogenesis.
Identification: cnn was identified in a screen for genomic DNA bound by homeotic proteins.
Genetic, cell biological and biochemical data suggest that the cnn product controls morphogenesis through mediating microtubule organization and is homeotically regulated.