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Gene Dmel\Gycα99B

General Information
Symbol	Dmel\Gycα99B	Species	D. melanogaster
Name	Guanylyl cyclase α-subunit at 99B	Annotation symbol	CG1912
Feature type	protein_coding_gene	FlyBase ID	FBgn0013972
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	dgc1, dgcα1, Gcα1, Gycα-99B, SGCα
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	99B7-99B7	Sequence location	3R:25,508,225..25,511,480 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Guanylyl cyclase α-subunit at 99B is referred to in FlyBase by the symbol Dmel\Gycα99B (CG1912, FBgn0013972). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: guanylate cyclase activity. There is experimental evidence that it is involved in the biological process: rhodopsin mediated signaling pathway; positive phototaxis. 11 alleles are reported. The phenotypes of these alleles are annotated with: photoreceptor cell R7; photoreceptor cell R8. It has one annotated transcript and one annotated polypeptide. Protein features are: Adenylyl cyclase class-3/4/guanylyl cyclase; Adenylyl cyclase class-3/4/guanylyl cyclase, conserved site; Haem NO binding associated; Heme-NO binding; NO signalling/Golgi transport ligand-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, during early larval stages, during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1641618_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Gycalpha99B. Gene sequence location is 3R:25508225..25511480.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 99B7-99B7   Limits computationally determined from genome sequence between P{PZ}l(3)0674306743 and P{PZ}ncd05884
Experimentally Determined Cytological Location
Cytogenetic map Notes References 63A1-63A2     (Yoshikawa, 1993.5.5) 99B-99B     (Shah, 1995.5.15) 99B-99B   (determined by in situ hybridisation)   (Shah and Hyde, 1995) 99B-99B   (determined by in situ hybridisation)   (Liu et al., 1995) 63A1-63A2   (determined by in situ hybridisation)   (Yoshikawa et al., 1993)
Experimentally Determined Recombination Data
Location	3- (Shah, 1995.5.15)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Gycα99B for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Gycα99B-RA FBtr0085453  NM_057740   2354   676
Additional Transcript Data & Comments
Reported size (kB)	4.1, 2.8, 2.4 (northern blot) (Shah and Hyde, 1995) 2.5 (northern blot) (Liu et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Gycα99B-PA   FBpp0084819   75.7   676   5.18     NP_477088   AAF56917
Additional Polypeptide Data & Comments
Reported size (kDa)	676 (aa); 76 (kD observed) (Liu et al., 1995) 676 (aa); 74 (kD observed); 76 (kD predicted) (Shah and Hyde, 1995)
Comments	Coexpression of Gycα99B protein and Gycβ100B protein in human kidney cells results in significant guanylyl cyclase activity. No activity is observed if either is introduced alone. Results indicate that Gycα99B protein and Gycβ100B protein are soluble guanylyl cyclase alpha and beta subunits that are capable of forming a functional guanylyl cyclase heterodimer. (Shah and Hyde, 1995)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Adenylyl cyclase class-3/4/guanylyl cyclase, conserved site (IPR018297) Adenylyl cyclase class-3/4/guanylyl cyclase (IPR001054) Heme-NO binding (IPR011644) NO signalling/Golgi transport ligand-binding domain (IPR024096) Haem NO binding associated (IPR011645)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567319   AE014297 AAF56917   AC008218   AI107858   AI109189   AI110081   AI389912   AI403275   AY060654 AAL28202   BI569349   BI569350   BI586630   BI587179   BI587905   BI588232   BI590532   BI605275   BI612054   BI612382   BI613299   BI619161   BI620024   BI624396   BI627811   BT133227   CO275703   CO309906   CO328655   EC198278   EC201985   EL881418   S57126 AAB25820   U27117 AAA87940
UniProtKB/Swiss-Prot	Q07093
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage & pupal stage     (Liu et al., 1995) adult stage   adult head   (Shah and Hyde, 1995, Liu et al., 1995) adult brain   (Liu et al., 1995) optic lobe   (Liu et al., 1995) thoracico-abdominal ganglion   (Liu et al., 1995)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference adult stage   medulla   (Shah and Hyde, 1995) optic lobe   (Shah and Hyde, 1995) retina   (Shah and Hyde, 1995) lamina   (Shah and Hyde, 1995) lobula plate   (Shah and Hyde, 1995) western blot Stage Tissue/Position (including subcellular localization) Reference adult stage   thoracico-abdominal ganglion   (Liu et al., 1995) adult head   (Liu et al., 1995) optic lobe   (Liu et al., 1995) adult brain   (Liu et al., 1995)
Additional Descriptive Data
	Gycα99 protein is detected in adult heads and at very low levels in bodies on western blots. Immunolocalization to adult tissue sections shows expression in the optic lobe, central brain, and thoracic ganglia. (Liu et al., 1995) Gycα99B protein is expressed in the retina and underlying optic lobes of the adult visual system. In addition, a diffuse pattern of staining is observed throughout the adult brain neuropil. (Shah and Hyde, 1995)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
guanylate cyclase complex, soluble inferred from direct assay (Shah and Hyde, 1995)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    213.95 Larval Midgut    5.5 Larval Hindgut    0.9 Larval Malpighian Tubules    1.4 Larval Fat Body    8.1 Larval Salivary Gland    1.3 Larval Trachea    0.85 Larval Carcass    1.35 Adult Head    226.9 Adult Eye    124.575 Adult Brain    1016.5 Adult Thoracic-Abdominal Ganglion    851 Adult Crop    7.8 Adult Midgut    5.2 Adult Hindgut    2.5 Adult Malpighian Tubules    1.5 Adult Fat Body    7.1 Adult Salivary Gland    14.2 Adult Heart    7.575 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    6.8 Adult Ovary    0.6 Adult Testis    4.3 Adult Male Accessory Gland    4.7 Adult Carcass    15.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    213.95 Larval Midgut    5.5 Larval Hindgut    0.9 Larval Malpighian Tubules    1.4 Larval Fat Body    8.1 Larval Salivary Gland    1.3 Larval Trachea    0.85 Larval Carcass    1.35 Adult Head    226.9 Adult Eye    124.575 Adult Brain  (1016.5) Adult Thoracic-Abdominal Ganglion  (851) Adult Crop    7.8 Adult Midgut    5.2 Adult Hindgut    2.5 Adult Malpighian Tubules    1.5 Adult Fat Body    7.1 Adult Salivary Gland    14.2 Adult Heart    7.575 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    6.8 Adult Ovary    0.6 Adult Testis    4.3 Adult Male Accessory Gland    4.7 Adult Carcass    15.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    213.95 Larval Midgut    5.5 Larval Hindgut    0.9 Larval Malpighian Tubules    1.4 Larval Fat Body    8.1 Larval Salivary Gland    1.3 Larval Trachea    0.85 Larval Carcass    1.35 Adult Head    226.9 Adult Eye    124.575 Adult Brain  1016.5 Adult Thoracic-Abdominal Ganglion    851 Adult Crop    7.8 Adult Midgut    5.2 Adult Hindgut    2.5 Adult Malpighian Tubules    1.5 Adult Fat Body    7.1 Adult Salivary Gland    14.2 Adult Heart    7.575 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    6.8 Adult Ovary    0.6 Adult Testis    4.3 Adult Male Accessory Gland    4.7 Adult Carcass    15.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    213.95 Larval Midgut    5.5 Larval Hindgut    0.9 Larval Malpighian Tubules    1.4 Larval Fat Body    8.1 Larval Salivary Gland    1.3 Larval Trachea    0.85 Larval Carcass    1.35 Adult Head    226.9 Adult Eye    124.575 Adult Brain    1016.5 Adult Thoracic-Abdominal Ganglion    851 Adult Crop    7.8 Adult Midgut    5.2 Adult Hindgut    2.5 Adult Malpighian Tubules    1.5 Adult Fat Body    7.1 Adult Salivary Gland    14.2 Adult Heart    7.575 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    6.8 Adult Ovary    0.6 Adult Testis    4.3 Adult Male Accessory Gland    4.7 Adult Carcass    15.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    213.95 Larval Midgut    5.5 Larval Hindgut    0.9 Larval Malpighian Tubules    1.4 Larval Fat Body    8.1 Larval Salivary Gland    1.3 Larval Trachea    0.85 Larval Carcass    1.35 Adult Head    226.9 Adult Eye    124.575 Adult Brain    1016.5 Adult Thoracic-Abdominal Ganglion    851 Adult Crop    7.8 Adult Midgut    5.2 Adult Hindgut    2.5 Adult Malpighian Tubules    1.5 Adult Fat Body    7.1 Adult Salivary Gland    14.2 Adult Heart    7.575 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    6.8 Adult Ovary    0.6 Adult Testis    4.3 Adult Male Accessory Gland    4.7 Adult Carcass    15.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    213.95 Larval Midgut    5.5 Larval Hindgut    0.9 Larval Malpighian Tubules    1.4 Larval Fat Body    8.1 Larval Salivary Gland    1.3 Larval Trachea    0.85 Larval Carcass    1.35 Adult Head    226.9 Adult Eye    124.575 Adult Brain  (1016.5) Adult Thoracic-Abdominal Ganglion  (851) Adult Crop    7.8 Adult Midgut    5.2 Adult Hindgut    2.5 Adult Malpighian Tubules    1.5 Adult Fat Body    7.1 Adult Salivary Gland    14.2 Adult Heart    7.575 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    6.8 Adult Ovary    0.6 Adult Testis    4.3 Adult Male Accessory Gland    4.7 Adult Carcass    15.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    213.95 Larval Midgut    5.5 Larval Hindgut    0.9 Larval Malpighian Tubules    1.4 Larval Fat Body    8.1 Larval Salivary Gland    1.3 Larval Trachea    0.85 Larval Carcass    1.35 Adult Head    226.9 Adult Eye    124.575 Adult Brain  1016.5 Adult Thoracic-Abdominal Ganglion    851 Adult Crop    7.8 Adult Midgut    5.2 Adult Hindgut    2.5 Adult Malpighian Tubules    1.5 Adult Fat Body    7.1 Adult Salivary Gland    14.2 Adult Heart    7.575 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    6.8 Adult Ovary    0.6 Adult Testis    4.3 Adult Male Accessory Gland    4.7 Adult Carcass    15.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    213.95 Larval Midgut    5.5 Larval Hindgut    0.9 Larval Malpighian Tubules    1.4 Larval Fat Body    8.1 Larval Salivary Gland    1.3 Larval Trachea    0.85 Larval Carcass    1.35 Adult Head    226.9 Adult Eye    124.575 Adult Brain    1016.5 Adult Thoracic-Abdominal Ganglion    851 Adult Crop    7.8 Adult Midgut    5.2 Adult Hindgut    2.5 Adult Malpighian Tubules    1.5 Adult Fat Body    7.1 Adult Salivary Gland    14.2 Adult Heart    7.575 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    6.8 Adult Ovary    0.6 Adult Testis    4.3 Adult Male Accessory Gland    4.7 Adult Carcass    15.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    226.9   NA  Eye    124.575   NA  Brain    1016.5   213.95  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    851   NA  Crop    7.8   5.5  Midgut    5.2   0.9  Hindgut    2.5   1.4  Malpighian Tubules    1.5   8.1  Fat Body    7.1   1.3  Salivary Gland    no informative data   NA  Heart    7.575   0.85  Trachea    NA   NA  VirginFemale Spermatheca    4.9   NA  InseminatedFemale Spermatheca    6.8   NA  Ovary    0.6   NA  Testis    4.3   NA  Male Accessory Gland    4.7   1.35  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    16 central nervous system, pupae P8    34 head, virgin 1-day female    40 head, virgin 4-day female    35 head, virgin 20-day female    30 head, mated 1-day female    33 head, mated 4-day female    32 head, mated 20-day female    25 head, mated 1-day male    77 head, mated 4-day male    62 head, mated 20-day male    53 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    4 carcass, larvae L3 wandering    3 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    16 central nervous system, pupae P8  (34) head, virgin 1-day female  (40) head, virgin 4-day female  (35) head, virgin 20-day female  (30) head, mated 1-day female  (33) head, mated 4-day female  (32) head, mated 20-day female    25 head, mated 1-day male  (77) head, mated 4-day male  (62) head, mated 20-day male  (53) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    4 carcass, larvae L3 wandering    3 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    16 central nervous system, pupae P8    34 head, virgin 1-day female    40 head, virgin 4-day female    35 head, virgin 20-day female    30 head, mated 1-day female    33 head, mated 4-day female    32 head, mated 20-day female    25 head, mated 1-day male    77 head, mated 4-day male    62 head, mated 20-day male    53 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    4 carcass, larvae L3 wandering    3 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    16 central nervous system, pupae P8    34 head, virgin 1-day female    40 head, virgin 4-day female    35 head, virgin 20-day female    30 head, mated 1-day female    33 head, mated 4-day female    32 head, mated 20-day female    25 head, mated 1-day male    77 head, mated 4-day male    62 head, mated 20-day male    53 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    4 carcass, larvae L3 wandering    3 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    16 central nervous system, pupae P8    34 head, virgin 1-day female    40 head, virgin 4-day female    35 head, virgin 20-day female    30 head, mated 1-day female    33 head, mated 4-day female    32 head, mated 20-day female    25 head, mated 1-day male    77 head, mated 4-day male    62 head, mated 20-day male    53 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    4 carcass, larvae L3 wandering    3 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    16 central nervous system, pupae P8  (34) head, virgin 1-day female  (40) head, virgin 4-day female  (35) head, virgin 20-day female  30 head, mated 1-day female  (33) head, mated 4-day female  32 head, mated 20-day female    25 head, mated 1-day male  (77) head, mated 4-day male  (62) head, mated 20-day male  (53) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    4 carcass, larvae L3 wandering    3 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    16 central nervous system, pupae P8    34 head, virgin 1-day female    40 head, virgin 4-day female    35 head, virgin 20-day female    30 head, mated 1-day female    33 head, mated 4-day female    32 head, mated 20-day female    25 head, mated 1-day male    77 head, mated 4-day male    62 head, mated 20-day male    53 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    4 carcass, larvae L3 wandering    3 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    16 central nervous system, pupae P8    34 head, virgin 1-day female    40 head, virgin 4-day female    35 head, virgin 20-day female    30 head, mated 1-day female    33 head, mated 4-day female    32 head, mated 20-day female    25 head, mated 1-day male    77 head, mated 4-day male    62 head, mated 20-day male    53 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    4 carcass, larvae L3 wandering    3 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, during early larval stages, during late pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    7 embryo 16-18hr    12 embryo 18-20hr    22 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    22 larva L2    8 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    10 pupae 3d postWPP    26 pupae 4d postWPP    27 adult male 01day    15 adult male 05day    16 adult male 30day    13 adult female 01day    8 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    7 embryo 16-18hr    12 embryo 18-20hr    22 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    22 larva L2    8 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    10 pupae 3d postWPP  26 pupae 4d postWPP  (27) adult male 01day    15 adult male 05day    16 adult male 30day    13 adult female 01day    8 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    7 embryo 16-18hr    12 embryo 18-20hr    22 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    22 larva L2    8 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    10 pupae 3d postWPP    26 pupae 4d postWPP    27 adult male 01day    15 adult male 05day    16 adult male 30day    13 adult female 01day    8 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    7 embryo 16-18hr    12 embryo 18-20hr    22 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    22 larva L2    8 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    10 pupae 3d postWPP    26 pupae 4d postWPP    27 adult male 01day    15 adult male 05day    16 adult male 30day    13 adult female 01day    8 adult female 05day    2 adult female 30day    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    7 embryo 16-18hr    12 embryo 18-20hr    22 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    22 larva L2    8 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    10 pupae 3d postWPP    26 pupae 4d postWPP    27 adult male 01day    15 adult male 05day    16 adult male 30day    13 adult female 01day    8 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    7 embryo 16-18hr    12 embryo 18-20hr    22 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    22 larva L2    8 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    10 pupae 3d postWPP  26 pupae 4d postWPP  27 adult male 01day    15 adult male 05day    16 adult male 30day    13 adult female 01day    8 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    7 embryo 16-18hr    12 embryo 18-20hr    22 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    22 larva L2    8 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    10 pupae 3d postWPP    26 pupae 4d postWPP    27 adult male 01day    15 adult male 05day    16 adult male 30day    13 adult female 01day    8 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    7 embryo 16-18hr    12 embryo 18-20hr    22 embryo 20-22hr    24 embryo 22-24hr    20 larva L1    22 larva L2    8 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    10 pupae 3d postWPP    26 pupae 4d postWPP    27 adult male 01day    15 adult male 05day    16 adult male 30day    13 adult female 01day    8 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_14 mE1_20_mRNA_expression_cluster_11
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013972 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1912-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Gycα99BGD9678 Other Phenotypes Allele neuroanatomy defective | recessive Gycα99B1 phototaxis defective Gycα99B1 Gycα99B2 Gycα99B3 Gycα99B5 visual behavior defective | recessive Gycα99B1 Gycα99B2 Gycα99B3 Gycα99B5 wild-type Gycα99Bhs.PG Phenotype manifest in Allele photoreceptor cell R7 Gycα99B1 photoreceptor cell R8 Gycα99B1
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of Gycα99B Class Mutagen Stocks Known lesion Gycα99B5-SZ-3291 1 -- Gycα99B1 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes Gycα99B2 ethyl methanesulfonate 0 -- Gycα99B3 ethyl methanesulfonate 0 -- Gycα99B4 ethyl methanesulfonate 0 -- Gycα99B5 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Gycα99B Class Mutagen Stocks Known lesion Gycα99BGD9678 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Gycα99BGL01038 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Gycα99BJF03176 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Gycα99BKK102774 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Gycα99Bhs.PG in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{Gycα99B.G} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{RS5}Gycα99B5-SZ-3291 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function
CV term Evidence References
guanylate cyclase activity inferred from direct assay (Shah and Hyde, 1995)
Biological Process
CV term Evidence References
positive phototaxis inferred from mutant phenotype (Gibbs et al., 2001) rhodopsin mediated signaling pathway inferred from mutant phenotype (Gibbs et al., 2001)
Cellular Component
CV term Evidence References
guanylate cyclase complex, soluble inferred from direct assay (Shah and Hyde, 1995)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
guanylate cyclase activity non-traceable author statement (Elphick and Jones, 1997, Shah, 1995.5.15, Yoshikawa, 1993.5.5, Morrison et al., 2000, Swiss-Prot Project Members, 1995.2.1) heme binding inferred from electronic annotation with InterPro:IPR011644 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
cGMP biosynthetic process inferred from electronic annotation with InterPro:IPR011645 (FlyBase Curators et al., 2004-) intracellular signal transduction inferred from electronic annotation with InterPro:IPR001054, InterPro:IPR018297 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Swiss-Prot Project Members, 1995.2.1) guanylate cyclase complex, soluble non-traceable author statement (Elphick and Jones, 1997)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Gycα99B allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0013972 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43493
Orthologs
OrthoDB Orthologs (44) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6HQF3T Dipteran inclusive ortholog search EOG6H9XTM Insect inclusive ortholog search EOG6FJ74S Arthropod inclusive ortholog search EOG60K6DS Metazoa inclusive ortholog search EOG66DZVQ
Orthologs in Drosophila Species (EOG6HQF3T)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Gycα99B     Drosophila simulans   Dsim\GD21450 Y   Drosophila sechellia   Dsec\GM12804 Y   Drosophila erecta   Dere\GG11679 Y   Drosophila yakuba   Dyak\GE23868 Y   Drosophila ananassae   Dana\GF23359 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA15127 Y   Drosophila persimilis   Dper\GL13901 Y   Drosophila willistoni   Dwil\GK11912 Y   Drosophila virilis   Dvir\GJ10614 Y   Drosophila mojavensis   Dmoj\GI23408 Y   Drosophila grimshawi   Dgri\GH18909 Y
Orthologs in non-Drosophila Dipterans (EOG6H9XTM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001457   Anopheles gambiae Malaria mosquito  Agam\AGAP000280   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ010180
Orthologs in non-Dipteran Insects (EOG6FJ74S)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB52929   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012541   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH21630   Atta cephalotes Leafcutter ant  Acep\ACEP25731   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17394   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15469   Linepithema humile Argentine ant  Lhum\LH13798   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24810   Solenopsis invicta Red fire ant  Sinv\SINV24778   Acyrthosiphon pisum Pea aphid  Apim\ACYPI008049   Bombyx mori Silkmoth  Bmor\BGIBMGA009300   Pediculus humanus Human body louse  Phum\PHUM588150   Tribolium castaneum Red flour beetle  Tcas\GLEAN_08018
Orthologs in non-Insect Arthropods (EOG60K6DS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Hr83   Ixodes scapularis Deer tick  Isca\ISCW011096   Ixodes scapularis Deer tick  Isca\ISCW024162
Orthologs in non-Arthropod Metazoa (EOG66DZVQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000116gn   Danio rerio Zebrafish  Drer\OTTDARG00000030219   Danio rerio Zebrafish  Drer\ENSDARG00000093185   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033652   Xenopus tropicalis Western clawed frog  Xtro\gucy1a2   Gallus gallus Domestic chicken  Ggal\GUCY1A2   Gallus gallus Domestic chicken  Ggal\GUCY1A3   Mus musculus House mouse  Mmus\Gucy1a2   Mus musculus House mouse  Mmus\Gucy1a3   Rattus norvegicus Norway rat  Rnor\Gucy1a3   Rattus norvegicus Norway rat  Rnor\Gucy1a2   Homo sapiens Human  Hsap\GUCY1A2   Homo sapiens Human  Hsap\GUCY1A3
Human Orthologs (2)
Gene OMIM HGNC Hsap\GUCY1A2     Hsap\GUCY1A3
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21450 Drosophila sechellia Dsec\GM12804 Drosophila erecta Dere\GG11679 Drosophila yakuba Dyak\GE23868 Drosophila ananassae Dana\GF23359 Drosophila pseudoobscura pseudoobscura Dpse\GA15127 Drosophila persimilis Dper\GL13901 Drosophila willistoni Dwil\GK11912 Drosophila virilis Dvir\GJ10614 Drosophila mojavensis Dmoj\GI23408 Drosophila grimshawi Dgri\GH18909
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	28748 y1 v1; P{TRiP.JF03176}attP2 36817 y1 sc* v1; P{TRiP.GL01038}attP2
Kyoto	125980 w1118; P{RS5}Gycα99B5-SZ-3291
VDRC	v107074 P{KK102774}VIE-260B v43711 w1118; P{GD9678}v43711
Genomic Clones ( 2 )
	BACR02O03 BACR10L12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 27 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH08311
Other clones	FI19414
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH05728 GH09539 GH21216 GH22678 HL01037 RH04165 RH26705 RH27666 RH28817 RH29370 RH40431 RH40831 RH42424 RH50140 RH51181 RH63948 RH69252 RH70853
Other clones	15149 97299 EK118344 EK219731 EK291062 IP11193 RH01443
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013972 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43493
Antibody Information
	polyclonal (Liu et al., 1995, Shah and Hyde, 1995)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Gycα99B CG1912 (Wang, 1999.11)
Source for database merge of	Source for merge of: Gycα99B Gycα63A (FlyBase, 1996-)
Additional comments	Source for merge of Gycα99B Gycα63A was sequence comparison (date:000202). (FlyBase, 1996-)
Other Comments
	Gyc-89Db shows no guanylyl cyclase activity when expressed in COS-7 cells in the absence of additional subunits, in the presence of either Mg2+ or Mn2+ cofactor. When Gyc-89Db and Gycα99B are co-expressed in COS-7 cells in the presence of either Mg2+ or Mn2+, no guanylyl cyclase activity is detected. (Langlais et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) The function of Gycα99B in the phototactic response is developmental, rather than physiological. (Gibbs et al., 2001) Isolated using cDNA fragments encoding the kinase-like and cyclase domains from the rat GC-A, GC-B and GC-C genes. (Liu et al., 1995) Gycα99B has been cloned and sequenced. It encodes a soluble guanylate cyclase subunit. (Liu et al., 1995) Gycα99B has been cloned and sequenced. It encodes a soluble guanylyl cyclase α subunit. (Shah and Hyde, 1995) Gycα99B has been cloned and sequenced. (Yoshikawa et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057740 RefSeq (Proteins) • NP_477088 DDBJ / EMBL / GenBank • AAA87940 AAB25820 AAF56917 AAL28202 AC008218 AI109189 AY060654 S57126 U27117 Entrez Gene - A searchable database of RefSeq genes. • 43493 UniProtKB/Swiss-Prot • Q07093
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Adenylyl cyclase class-3/4/guanylyl cyclase, conserved site (IPR018297) Adenylyl cyclase class-3/4/guanylyl cyclase (IPR001054) Heme-NO binding (IPR011644) NO signalling/Golgi transport ligand-binding domain (IPR024096) Haem NO binding associated (IPR011645)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0013972 DRSC - Results from RNAi screens. • FBgn0013972 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013972 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1912-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013972 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43493 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43493 modMine - Data generated by the modENCODE project. • FBgn0013972
Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym	CG1912 (Morrison et al., 2000, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Perkins et al., 2009) dgc1 (Davies, 2000, Yoshikawa, 1993.5.5, Gibbs and Truman, 1998, Yoshikawa et al., 1993, Gibbs and Truman, 1999) Dgc1 (Shah and Hyde, 1995, ) dgca1 (Shah, 1995.5.15, ) dgcα1 (Shah and Hyde, 1995, Liu et al., 1995, Riedl et al., 2005) Dgcα1 (Zhang et al., 2002) Gcα1 (Gibbs et al., 2001, Gibbs, 2001) GCα1 (Zelhof et al., 2003) GYCa 99B (Morrison et al., 2000) gycalpha (Siddiqi, 1998) Gycα63A   Gycα-99B (Morton, 2004, Langlais et al., 2004) Gycα99B (Adryan et al., 2007) sGC (Tinette et al., 2007) SGCα (Elphick and Jones, 1998, Elphick and Jones, 1997) sGCα1 (Zhang et al., 2002) sGCα99B (Kanao et al., 2012) unnamed (Liu et al., 1993)
Name Synonym	guanylate cyclase 99B (Brenman et al., 2001) Guanyl cyclase α-subunit at 63A   Guanylyl cyclase α-subunit at 99B   soluble guanylate cyclase (Tinette et al., 2007) Soluble Guanylyl Cyclase α (Elphick and Jones, 1997)
Secondary FlyBase IDs
	FBgn0010227
References ( 42 )
Generate a list of	All references relating to this gene ( 42 )
List References by type	Research paper  ( 16 ) Supplementary material  ( 5 ) Review  ( 2 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 4 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 )
Recent research papers ( 1 )
	Kanao et al., 2012, PLoS ONE 7(2): e30958 The Nitric Oxide-Cyclic GMP Pathway Regulates FoxO and Alters Dopaminergic Neuron Survival in Drosophila. [FBrf0217639] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...