• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Dmel\Gyc76C

General Information
Symbol	Dmel\Gyc76C	Species	D. melanogaster
Name	Guanylyl cyclase at 76C	Annotation symbol	CG8742
Feature type	protein_coding_gene	FlyBase ID	FBgn0250792
Created / Updated	2008-02-14/2008-02-14
Genomic Location
Chromosome (arm)	3L	Recombination map	3-
Cytogenetic map	76C3-100	Sequence location	3L:19,712,626..19,786,663 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Guanylyl cyclase at 76C is referred to in FlyBase by the symbol Gyc76C (CG8742, FBgn0250792). It has the cytological map location 76C3-100. Its sequence location is 3L:19712626..19786663. Its molecular function is described as: guanylate cyclase activity; receptor activity; ATP binding; protein-tyrosine kinase activity. It is involved in the biological processes: signal transduction; motor axon guidance; axon midline choice point recognition; protein amino acid phosphorylation; intracellular signaling cascade; cyclic nucleotide biosynthetic process. 30 alleles are reported. The phenotypes of these alleles are annotated with: anterior fascicle; abdominal posterior fascicle. It has 3 annotated transcripts and 3 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 76C3-100   Limits computationally determined from genome sequence between P{lacW}l(3)L3809L3809 and P{lacW}l(3)L1243L1243&P{lacW}Mi-2j3D4; Left limit from (method unavailable) (FBrf0067338) Right limit from (method unavailable) (FBrf0067338)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 76C-76C   (Liu, 1995.3.27) 76C-76C (determined by in situ hybridisation)   (Liu et al., 1995) 76C-76C (determined by in situ hybridisation)   (McNeil et al., 1995)
Experimentally Determined Recombination Data
Location	3- (Liu, 1995.3.27)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Gyc76C for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Gyc76C-RA FBtr0100155  NM_079441   6243   1525 Gyc76C-RB FBtr0100156  NM_001007096   6195   1525 Gyc76C-RC FBtr0100157  NM_001007095   6356   1525
Additional Transcript Data & Comments
Reported size (kB)	7.0 (northern blot) (Liu et al., 1995, McNeil et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Gyc76C-PA   FBpp0099506   170.5   1525   7.24     NP_524165   AAF49117 Gyc76C-PB   FBpp0099507   170.5   1525   7.24     NP_001007097   AAV50029 Gyc76C-PC   FBpp0099508   170.5   1525   7.24     NP_001007096   AAV50027
Additional Polypeptide Data & Comments
Reported size (kD)	1525 (aa) (McNeil et al., 1995, Liu et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, core (IPR000719) Adenylyl cyclase class-3/4/guanylyl cyclase (IPR001054) Tyrosine protein kinase (IPR001245) Extracellular ligand-binding receptor (IPR001828) Protein kinase-like (IPR011009) Serine/threonine protein kinase-related (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201965   AA392994   AA817488   AA949302   AA952218   AI455312   AI533591   AW942224   AX093945   BI237986   BI374461   BI632814   BI633808   BP540945   BT011062   CK660050   CL527683   CO332726   CZ466683   CZ469512   CZ472083   CZ472279   CZ474344   CZ474462   CZ478184   CZ480854   CZ482848   CZ483488   CZ485662   CZ487535   CZ487666   CZ488374   CZ488667   CZ488668   EC051988   EC052007   EC054993   EC056314   EC061891   EC065502   EC065728   EC069566   EC081001   EC082742   EC083470   EC212268   EC215466   EC248104   EC255106   EC259942   L35598 AAA74408   U23485 AAA85858
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q24051 Q7JQ32
Maps to
Does NOT map to
Identified with	LD12174 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage-adult stage   (Liu et al., 1995)  adult stage  ventriculus (Liu et al., 1995)  adult stage  adult brain (Liu et al., 1995)  adult stage  cardia (Liu et al., 1995)  embryonic stage | late  larval somatic muscle (McNeil et al., 1995)  adult stage  retina (Liu et al., 1995)  embryonic stage | late  larval visceral muscle (McNeil et al., 1995)  oogenesis stage,adult stage  oocyte (Liu et al., 1995)  adult stage  optic lobe (Liu et al., 1995)  adult stage  pupal/adult digestive system (Liu et al., 1995)  embryonic stage | early   (McNeil et al., 1995)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Gyc76CEY02825 Gyc76Ce03087 lethal Gyc76CCB03117 Gyc76CKG03723ex173 Gyc76CKG03723ex33 lethal | recessive Gyc76CL0090 Other Phenotypes Allele neuroanatomy defective Gyc76CKG03723 Gyc76CKG03723ex33 Gyc76CL0090 neuroanatomy defective (with Df(3L)5126) Gyc76CKG03723ex173 neuroanatomy defective (with Gyc76CKG03723ex33) Gyc76CKG03723ex173 neuroanatomy defective (with Gyc76CKG03723ex173) Gyc76CKG03723ex33 neuroanatomy defective | semidominant Gyc76CKG03723ex173 neuroanatomy defective, with Scer\GAL4elav.PLu Gyc76CD945A.Scer\UAS.T:Hsap\MYC Gyc76CScer\UAS.T:Hsap\MYC Sterility Allele fertile Gyc76CEY02825 Gyc76Ce03087 Phenotype manifest in Allele anterior fascicle Gyc76CL0090 Gyc76CKG03723 Gyc76CKG03723ex33 Gyc76CKG03723ex173 abdominal posterior fascicle Gyc76CL0090 Gyc76CKG03723 Gyc76CKG03723ex33 Gyc76CKG03723ex173 anterior fascicle (with Gyc76CKG03723ex173) Gyc76CKG03723ex33 abdominal posterior fascicle (with Gyc76CKG03723ex173) Gyc76CKG03723ex33 anterior fascicle (with Gyc76CKG03723ex33) Gyc76CKG03723ex173 abdominal posterior fascicle (with Gyc76CKG03723ex33) Gyc76CKG03723ex173 anterior fascicle (with Df(3L)5126) Gyc76CKG03723ex173 abdominal posterior fascicle (with Df(3L)5126) Gyc76CKG03723ex173 anterior fascicle, with Scer\GAL4elav.PLu Gyc76CScer\UAS.T:Hsap\MYC Gyc76CD945A.Scer\UAS.T:Hsap\MYC abdominal posterior fascicle, with Scer\GAL4elav.PLu Gyc76CScer\UAS.T:Hsap\MYC Gyc76CD945A.Scer\UAS.T:Hsap\MYC axon & RP3 neuron, with Scer\GAL4elav.PLu Gyc76CD945A.Scer\UAS.T:Hsap\MYC
Classical Alleles ( 24 )
For All Classical Alleles Show
Allele of Gyc76C Class Mutagen Stocks Known lesion Gyc76CA377 0 -- Gyc76CCB03117 P-element activity 0 Yes Gyc76CDG02611 P-element activity 1 -- Gyc76CEY02825 P-element activity 1 -- Gyc76CKG03723 1 Yes Gyc76CKG03723ex144 Δ2-3 0 Yes Gyc76CKG03723ex173 loss of function Δ2-3 0 Yes Gyc76CKG03723ex33 loss of function Δ2-3 0 Yes Gyc76CL0090 0 Yes Gyc76Cc00732 piggyBac transposase 1 -- Gyc76Cc04783 piggyBac transposase 1 -- Gyc76Cd01197 P-element activity 1 -- Gyc76Cd01512 P-element activity 0 -- Gyc76Cd05142 P-element activity 1 -- Gyc76Cd05329 P-element activity 0 -- Gyc76Ce00296 piggyBac transposase 1 -- Gyc76Ce03087 piggyBac transposase 2 -- Gyc76Cf00380 piggyBac transposase 0 -- Gyc76Cf01002 piggyBac transposase 1 -- Gyc76Cf03109 piggyBac transposase 0 -- Gyc76Cf04947 piggyBac transposase 0 -- Gyc76Cf05061 piggyBac transposase 1 -- Gyc76Cf05747 piggyBac transposase 1 -- Gyc76Cf06025 piggyBac transposase 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Gyc76C Class Mutagen Stocks Known lesion Gyc76CD945A.Scer\UAS.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion in vitro construct | amino acid replacement in vitro construct | site directed mutagenesis 0 Yes Gyc76CGD10476 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes Gyc76CGD14428 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes Gyc76CGD2544 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes Gyc76CGD48 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes Gyc76CScer\UAS.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3L)XS705
Disrupted in	Df(3L)5126 (Ayoob et al., 2004) Df(3L)Exel6135 (Ryder, 2004.4.26)
Not disrupted in	Df(3L)BSC1 (Bloomington Drosophila Stock Center, 1999.2.23) Df(3L)BSC2 (Bloomington Drosophila Stock Center, 1999.2.23)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10476} NA P{GD14428} NA P{GD2544} NA P{GD48} NA P{UAS-Gyc76C.D945A} NA P{UAS-Gyc76C.MYC} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{5HPw+}Gyc76CA377 NA PBac{PB}Gyc76Cc00732 NA PBac{PB}Gyc76Cc04783 NA PBac{RB}Gyc76Ce00296 NA PBac{RB}Gyc76Ce03087 NA PBac{WH}Gyc76Cf00380 NA PBac{WH}Gyc76Cf01002 NA PBac{WH}Gyc76Cf03109 NA PBac{WH}Gyc76Cf04947 NA PBac{WH}Gyc76Cf05061 NA PBac{WH}Gyc76Cf05747 NA PBac{WH}Gyc76Cf06025 NA P{PTT-GB}Gyc76CCB03117 NA P{SUPor-P}Gyc76CKG03723 NA P{wHy}Gyc76CDG02611 NA insertion of enhancer trap P{lacW}Gyc76CL0090 No insertion of mobile activating element P{EPgy2}Gyc76CEY02825 NA P{GSV1}EP-PEP-P NA P{XP}Gyc76Cd01197 NA P{XP}Gyc76Cd01512 NA P{XP}Gyc76Cd05142 NA P{XP}Gyc76Cd05329 NA
Related Comments
	Please look at the allele reports for the complete phenotype data A Gyc76C cDNA has been cloned and sequenced. Gyc76C encodes a receptor guanylyl cyclase. (McNeil et al., 1995) Gyc76C has been cloned and sequenced. It encodes a receptor-type guanylate cyclase. (Liu et al., 1995)
Gene Ontology: Function, Process & Cellular Component ( 11 )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR017442 (FlyBase Curators et al., 2004-)
guanylate cyclase activity inferred from direct assay (McNeil et al., 1995) non-traceable author statement (McNeil, 1994) inferred from sequence or structural similarity with MGD:Npr1; MGI:MGI:97371 (FlyBase, 1992-)
protein tyrosine kinase activity inferred from electronic annotation with InterPro:IPR001245 (FlyBase Curators et al., 2004-)
receptor activity inferred from direct assay (McNeil et al., 1995) non-traceable author statement (McNeil, 1994) inferred from sequence or structural similarity with UniProtKB:P55202 (FlyBase, 1992-)
Biological Process
CV term Evidence References
axon midline choice point recognition inferred from genetic interaction with FLYBASE:Sema-1a; FB:FBgn0011259 inferred from genetic interaction with FLYBASE:plexA; FB:FBgn0025741 (Ayoob et al., 2004)
cyclic nucleotide biosynthetic process inferred from electronic annotation with InterPro:IPR001054 (FlyBase Curators et al., 2004-)
intracellular signaling cascade inferred from electronic annotation with InterPro:IPR001054 (FlyBase Curators et al., 2004-)
motor axon guidance inferred from mutant phenotype (Ayoob et al., 2004)
protein amino acid phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR017442 (FlyBase Curators et al., 2004-)
signal transduction inferred from sequence or structural similarity with UniProtKB:P55202 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
plasma membrane inferred from sequence or structural similarity with FLYBASE:Gyc32E; FB:FBgn0010197 (McNeil et al., 1995) inferred from sequence or structural similarity with FLYBASE:Gyc32E; FB:FBgn0010197 (Liu et al., 1995) non-traceable author statement (McNeil, 1994) inferred from sequence or structural similarity with UniProtKB:P55202 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene   gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Gyc76C allele Gene References unspecified Sema-1a (Ayoob et al., 2004) plexA (Ayoob et al., 2004)
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10712 Dere\GG13375 Dgri\GH16824 Dmoj\GI11917 Dpse\GA28220 Dsec\GM16468 Dsim\GD12253 Dvir\GJ13791 Dwil\GK25717 Dyak\GE22469
Curated drosophilid orthologs	Dmir\CG8742 (Thornton, 2005.12.20)
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 22 )
Harvard	- P{XP}Gyc76Cd01197 - P{XP}Gyc76Cd05142 - PBac{PB}Gyc76Cc00732 - PBac{PB}Gyc76Cc04783 - PBac{RB}Gyc76Ce00296 - PBac{RB}Gyc76Ce03087 - PBac{WH}Gyc76Cf01002 - PBac{WH}Gyc76Cf05061