FB2022_02, released March 29, 2022

Gene: Dmel\imd

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General Information
Symbol
Dmel\imd
Species
D. melanogaster
Name
immune deficiency
Annotation Symbol
CG5576
Feature Type
protein_coding_gene
FlyBase ID
FBgn0013983
Gene Model Status
Current
Stock Availability
16 publicly available
Gene Summary
immune deficiency (imd) encodes a canonic component of the immune deficiency pathway, which regulates the antibacterial response and other less characterized cellular processes. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

BG5

Key Links
Genomic Location
Cytogenetic map
55C8-55C8
Sequence location
Recombination map
2-85
RefSeq locus
NT_033778 REGION:18409796..18413599
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (15 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables death domain binding
inferred from physical interaction with FLYBASE:Fadd; FB:FBgn0038928
(Naitza et al., 2002)
enables protein binding
inferred from physical interaction with FLYBASE:pirk; FB:FBgn0034647
(Aggarwal et al., 2008)
inferred from physical interaction with UniProtKB:Q9V3B4
(assigned by UniProt )
(Naitza et al., 2002)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (13 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
involved_in antibacterial humoral response
inferred from mutant phenotype
(Corbo and Levine, 1996, Lemaitre et al., 1995)
involved_in humoral immune response
inferred from mutant phenotype
(Corbo and Levine, 1996)
involved_in immune response
inferred from mutant phenotype
(Bernal and Kimbrell, 2000)
involved_in nervous system development
inferred from mutant phenotype
(Koizumi et al., 2007)
involved_in peptidoglycan recognition protein signaling pathway
inferred from high throughput mutant phenotype
(Kleino et al., 2005)
inferred from mutant phenotype
(Chen et al., 2017, Paquette et al., 2010, Ertürk-Hasdemir et al., 2009, Valanne et al., 2007, Leulier et al., 2000)
inferred from mutant phenotype
(assigned by UniProt )
(Uttenweiler-Joseph et al., 1998)
inferred from genetic interaction with FLYBASE:Dredd; FB:FBgn0020381, FLYBASE:Fadd; FB:FBgn0038928
(Zhou et al., 2005)
involved_in positive regulation of antibacterial peptide biosynthetic process
inferred from mutant phenotype
(Manfruelli et al., 1999, Wu and Anderson, 1998)
involved_in positive regulation of antibacterial peptide production
inferred from genetic interaction with FLYBASE:Tl; FB:FBgn0262473
(Lemaitre et al., 1996)
involved_in positive regulation of biosynthetic process of antibacterial peptides active against Gram-negative bacteria
inferred from high throughput mutant phenotype
(Kleino et al., 2005)
involved_in positive regulation of defense response to virus by host
inferred from mutant phenotype
(Avadhanula et al., 2009)
involved_in positive regulation of innate immune response
inferred from mutant phenotype
(Goto et al., 2008)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in defense response to Gram-negative bacterium
traceable author statement
(Brennan and Anderson, 2004, Janssens and Beyaert, 2003)
involved_in intracellular signal transduction
traceable author statement
(Brennan and Anderson, 2004)
involved_in response to bacterium
non-traceable author statement
(Silverman, 2003)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
immune deficiency (imd) encodes a canonic component of the immune deficiency pathway, which regulates the antibacterial response and other less characterized cellular processes. [Date last reviewed: 2019-03-07]
Pathway (FlyBase)
Imd Signaling Pathway Core Components -
The immune deficiency (Imd) pathway primarily mediates the humoral immune response to Gram-negative bacteria. Activation of the Imd pathway by diaminopimelic acid-type peptidoglycan initiates a signaling cascade that ultimately results in the release of the NFκB-like factor Rel from auto-inhibition and its translocation into the nucleus to activate the transcription of antimicrobial peptides. (Adapted from FBrf0224587 and FBrf0238555.)
Protein Function (UniProtKB)
Essential for the imd/NF-kappa-B (Imd) humoral and epithelial immune response to Gram-negative bacteria (PubMed:7568155, PubMed:8808632, PubMed:11266367, PubMed:12433364). Functions as an adapter protein that transduces immunity signals from the activation of pathogen recognition receptors (PRRs) by bacterial infection to the Imd signaling pathway (PubMed:7568155, PubMed:11269502, PubMed:11703941, PubMed:12433364, PubMed:20122400). Binding of diaminopimelic acid-type (DAP-type) bacterial peptidoglycans (PGN) causes multimerization or clustering of PGRP receptors which activate the Imd cascade probably by recruiting imd, Fadd and Dredd to the receptor complex (PubMed:11269502, PubMed:12433364, PubMed:20122400). Once in proximity, Dredd cleaves imd in a Fadd-dependent manner to enable its association and activation by the ubiquitin E3-ligase DIAP2 (PubMed:20122400). The activated form of imd recruits and activates the Tab2/Tak1 complex thus acting upstream of the IKK complex (ird5 and key) to activate Rel and induce the expression of antimicrobial peptides such as Def, Dpt and Cecropin (PubMed:7568155, PubMed:8808632, PubMed:11703941, PubMed:12433364, PubMed:19837371, PubMed:20122400, PubMed:11266367). Also able to inhibit the viral replication of the Sindbis virus (PubMed:19763182). Involved in promoting the polyubiquitination and stability of faf which in turn, regulates the Imd pathway by controlling imd polyubiquitination and/or stability; they may therefore form a regulatory feedback mechanism within the Imd pathway (PubMed:23919485). Not required for the cuticle melanization immune response to bacterial challenge (PubMed:11266367).
(UniProt, Q7K4Z4)
Summary (Interactive Fly)

a death domain protein involved in signal transduction during antibacterial immune response

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\imd for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7K4Z4)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
imd-RA
FBtr0086725
NM_133166
1320
273
imd-RB
FBtr0345891
NM_001299643
1518
273
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
imd-PA
FBpp0085904
29.9
273
6.16
NP_573394
AAF57692
imd-PB
FBpp0311815
29.9
273
6.16
NP_001286572
AHN56367
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

273 aa isoforms: imd-PA, imd-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with Fadd (PubMed:12433364). Interacts with faf (PubMed:23919485). Interacts with Rpt6 (PubMed:25027767). Interacts (via N-terminus) with Usp2 (via N-terminus) (PubMed:25027767). Interacts (via N-terminus) with scny (via C-terminus) (PubMed:19837371). The N-terminally cleaved form interacts (via IAP-Binding motif) with Diap2 (via the BIR2 and BIR3 domains); the interaction promotes ubiquitination by Diap2 and the E2 ligases Uev1A, ben and Ubc5 (PubMed:20122400).

(UniProt, Q7K4Z4)
Post Translational Modification

Phosphorylated.

Caspase-mediated cleavage is required for activation and function; upon immune stimulation, peptidoglycans (PGN) induce proteolytic cleavage by caspases such as Dredd leading to its ubiquitination.

Ubiquitination is essential for function; after PGN-induced caspase-mediated cleavage the N-terminally cleaved imd interacts with the E3 ligase Diap2 leading to polyubiquitination of 'Lys-63'-linked chains involving the E2 complex members Uev1A, ben and Ubc5 (PubMed:20122400). These 'Lys-63' chains stabilize imd and may serve as scaffolds to recruit and activate the key kinases TAK1 and IKK (PubMed:20122400). Under normal unchallenged conditions, scny deubiquitinates the activating 'Lys-63'-linked chains to prevent signal transduction and this is also likely to promote the polyubiquitination of 'Lys-48'-linked chains which act as 'tags' for proteosomal degradation (PubMed:19837371). Usp2 then deubiquitinates the 'Lys-48'-linked chains and this promotes degradation of imd probably by allowing interaction between imd and the proteasome (PubMed:25027767).

(UniProt, Q7K4Z4)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\imd using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult Malpighian tubule
(McGettigan et al., 2005)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\imd in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of imd
Transgenic constructs containing regulatory region of imd
Aberrations (Deficiencies and Duplications) ( 8 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die before end of larval stage, with Scer\GAL4da.G32
partially lethal - majority die | RU486 conditional, with Scer\GAL4da.Switch.PT
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4hs.PB
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Sterility
Allele
fertile
Other Phenotypes
Allele
abnormal immune response
abnormal immune response, with Scer\GAL4yolk
abnormal immune response (with Df(2R)1.1)
abnormal immune response (with Df(2R)1.2)
abnormal immune response (with Df(2R)2.1)
abnormal immune response (with Df(2R)2.2)
abnormal immune response (with Df(2R)C5)
abnormal immune response | adult stage
abnormal immune response | adult stage, with Scer\GAL4c564
abnormal immune response | adult stage | RU486 conditional, with Scer\GAL4da.Switch.PT
abnormal immune response | recessive
abnormal neuroanatomy | third instar larval stage
abnormal starvation stress response | adult stage | RU486 conditional, with Scer\GAL4da.Switch.PT
abnormal starvation stress response | adult stage | RU486 conditional, with Scer\GAL4elav.Switch.PO
abnormal starvation stress response | adult stage | RU486 conditional, with Scer\GAL4GS.Uro
abnormal stress response | adult stage | RU486 conditional, with Scer\GAL4da.Switch.PT
decreased body weight | adult stage | RU486 conditional, with Scer\GAL4da.Switch.PT
decreased feeding behavior | adult stage | RU486 conditional, with Scer\GAL4da.Switch.PT
decreased feeding behavior | adult stage | RU486 conditional, with Scer\GAL4elav.Switch.PO
increased cell death, with Scer\GAL4hs.PB
increased mortality | adult stage |  :RU486 conditional | conditional, with Scer\GAL4da.Switch.PT
increased mortality | adult stage | conditional
increased mortality | conditional
radiation resistant
radiation sensitive, with Scer\GAL4hs.PB
short lived
short lived | adult stage | conditional
short lived | conditional
short lived | RU486 conditional, with Scer\GAL4da.Switch.PT
short lived | RU486 conditional, with Scer\GAL4TIGS-2
some die during larval stage, with Scer\GAL4da.G32
Phenotype manifest in
Allele
embryonic/larval fat body, with Scer\GAL4hs.PB
embryonic/larval neuromuscular junction | third instar larval stage
presynaptic active zone | third instar larval stage
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (0)
No records found.
Caenorhabditis elegans (Nematode, roundworm) (0)
No records found.
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 4 )
    Allele
    Disease
    Interaction
    References
    imd1
    exacerbates  carcinoma
    modeled by dlg1A40.2
    (Parvy et al., 2019)
    imdUAS.cGa
    exacerbates  colorectal cancer
    modeled by Ras85DV12.UAS
    (Bangi et al., 2012)
    imdKK109863
    exacerbates  carcinoma
    modeled by dlg1A40.2
    (Parvy et al., 2019)
    imdHMS00253
    ameliorates  autism spectrum disorder
    modeled by Kdm5HM05155
    (Chen et al., 2019)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    RNA-RNA
    Physical Interaction
    Assay
    References
    imd - mir-310
    luminiscence technology
    (Robins et al., 2005)
    protein-protein
    Physical Interaction
    Assay
    References
    imd
    x-ray fiber diffraction, electron microscopy, electrophoretic mobility-based method, anti tag western blot
    (Cai et al., 2021, Kleino et al., 2017)
    imd - Diap2
    anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, pull down
    (Meinander et al., 2012, Paquette et al., 2010)
    imd - Dredd
    enzymatic study, western blot
    (Kim et al., 2014)
    imd - Fadd
    anti tag coimmunoprecipitation, anti tag western blot, two hybrid, anti bait coimmunoprecipitation
    (Petkau et al., 2017, Guntermann and Foley, 2011, Naitza et al., 2002)
    imd - Nopp140
    experimental knowledge based
    (Guruharsha et al., 2011)
    imd - PGRP-LC
    anti tag coimmunoprecipitation, anti tag western blot
    (Aggarwal et al., 2008, Kaneko et al., 2006, Choe et al., 2005)
    imd - PGRP-LE
    anti tag coimmunoprecipitation, anti tag western blot
    (Kaneko et al., 2006)
    imd - Rpt6
    anti tag coimmunoprecipitation, anti tag western blot
    (Engel et al., 2014)
    imd - Tak1
    enzymatic study, autoradiography, inferred by author
    (Chen et al., 2017)
    imd - Usp2
    anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot
    (Engel et al., 2014)
    imd - faf
    anti tag coimmunoprecipitation, anti tag western blot
    (Yagi et al., 2013)
    imd - pirk
    anti tag coimmunoprecipitation, anti tag western blot
    (Aggarwal et al., 2008, Kleino et al., 2008, Lhocine et al., 2008)
    imd - scny
    anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, pull down
    (Thevenon et al., 2009)
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    imd
    enhanceable
    GluRIIA
    (Harris et al., 2018)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Diedel
    suppressible
    imd
    (Lamiable et al., 2016)
    dlg1
    suppressible
    imd
    (Parvy et al., 2019)
    dlg1
    enhanceable
    imd
    (Parvy et al., 2019)
    dlg1
    enhanceable
    Def|imd
    (Parvy et al., 2019)
    dlg1
    suppressible
    Def|imd
    (Parvy et al., 2019)
    pbl
    suppressible
    imd
    (Gregory et al., 2007)
    PGRP-LF
    suppressible
    imd
    (Maillet et al., 2008)
    Ras85D
    enhanceable
    imd
    (Bangi et al., 2012)
    External Data
    Subunit Structure (UniProtKB)
    Interacts with Fadd (PubMed:12433364). Interacts with faf (PubMed:23919485). Interacts with Rpt6 (PubMed:25027767). Interacts (via N-terminus) with Usp2 (via N-terminus) (PubMed:25027767). Interacts (via N-terminus) with scny (via C-terminus) (PubMed:19837371). The N-terminally cleaved form interacts (via IAP-Binding motif) with Diap2 (via the BIR2 and BIR3 domains); the interaction promotes ubiquitination by Diap2 and the E2 ligases Uev1A, ben and Ubc5 (PubMed:20122400).
    (UniProt, Q7K4Z4 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Imd Signaling Pathway Core Components -
    The immune deficiency (Imd) pathway primarily mediates the humoral immune response to Gram-negative bacteria. Activation of the Imd pathway by diaminopimelic acid-type peptidoglycan initiates a signaling cascade that ultimately results in the release of the NFκB-like factor Rel from auto-inhibition and its translocation into the nucleus to activate the transcription of antimicrobial peptides. (Adapted from FBrf0224587 and FBrf0238555.)
    Metabolic Pathways
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-85
    Cytogenetic map
    55C8-55C8
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    55C8-55C8
    Limits computationally determined from genome sequence between P{PZ}sbb04525&P{EP}EP1081EP1081 and P{lacW}l(2)08770k04808
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-85

    (FlyBase, 2016)

    2-89.8

    (Comeron et al., 2012)

    2-

    (Corbo and Levine, 1995)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (16)
    Genomic Clones (11)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (11)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Chen et al., 2017, Paquette et al., 2010, Naitza et al., 2002)
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: imd shadok

    (Ferrandon, 2001.3.6)

    Source for identity of: BG5 CG5576

    (FlyBase, 1996-)
    Source for database merge of

    Source for merge of: imd BG5

    (De Gregorio et al., 2001)

    Source for merge of: imd anon-WO0172774.166

    (FlyBase, 1996-)
    Additional comments

    Source for identity of BG5 CG5576 was sequence comparison ( date:000327 ).

    (FlyBase, 1996-)

    Source for merge of imd anon-WO0172774.166 was sequence comparison ( date:051113 ).

    (FlyBase, 1996-)
    Other Comments

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a specific decrease in AttA activity in response to heat-killed E.coli when assayed in S2 cells. imd functions upstream of Iap2 in the fat body, as shown by the ability of Iap2 dsRNA to inhibit transcriptional activation of Dpt by imd.

    (Kleino et al., 2005)

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    (Kiger et al., 2003)

    imd regulates the antibacterial response.

    (Ferrandon et al., 2001)

    Mutants show no detectable induction of Dpt, CecA1 and CecA2. Attacin levels are reduced compared to wild type and Drs induction is normal.

    (Lu et al., 2001)

    Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.

    (McDonald and Rosbash, 2001)

    Mutants exhibit a constitutive Dif nuclear localisation.

    (Wu and Anderson, 1998)

    Mutations that affect the synthesis of antimicrobial peptides dramatically lower the resistance of flies to infection.

    (Lemaitre et al., 1996)

    The imd locus was identified by a mutation which abrogates (in larvae) or reduces (in adults) the induction of humoral immune response genes. Failure of induction results in mortality due to sepsis after challenge with live bacteria.

    (Corbo and Levine, 1995)

    When challenged with bacteria mutants show a lower survival rate that wild type flies. In contrast to the antibacterial peptides the antifungal peptide Drs remains inducible in a homozygous imd mutant background. These results point to the existance of two different pathways leading to the expression of two types of target genes, encoding either the antibacterial peptides or the antifungal peptide Drs.

    (Lemaitre et al., 1995)
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 50 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL - MARRVEL (model organism gene)
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (12)
    Reported As
    Symbol Synonym
    BG5
    (Georgel et al., 2001, Lemaitre, 2000.12.20)
    CG5576
    (Douglas et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Kleino et al., 2005, De Gregorio et al., 2001, Irving et al., 2001)
    IMD
    (Fabian et al., 2021, Harnish et al., 2021, Madi et al., 2021, Ma et al., 2021, Rosendo Machado et al., 2021, Salem Wehbe et al., 2021, Schneider and Imler, 2021, Sciambra and Chtarbanova, 2021, Colombani and Andersen, 2020, Grenier and Leulier, 2020, Jasper, 2020, Jeon et al., 2020, Lim et al., 2020, Wang et al., 2020, Warsaba et al., 2020, Zhao and Karpac, 2020, Chen et al., 2019, Galenza and Foley, 2019, Bachtel et al., 2018, Kounatidis and Chtarbanova, 2018, Palmer et al., 2018, Westfall et al., 2018, Mussabekova et al., 2017, Tavignot et al., 2017, Trinder et al., 2017, Iyer et al., 2016, Kumar et al., 2016, Neyen et al., 2016, Orme et al., 2016, Xiong et al., 2016, El Chamy et al., 2015, Goto et al., 2014, Imler, 2014, Kleino and Silverman, 2014, Xu and Cherry, 2014, Ferrandon, 2013, Fukuyama et al., 2013, Kingsolver et al., 2013, Lee and Brey, 2013, Merkling and van Rij, 2013, Bier and Guichard, 2012, Kounatidis and Ligoxygakis, 2012, Longworth et al., 2012, Meinander et al., 2012, Vandenabeele and Bertrand, 2012, Akhouayri et al., 2011, Douglas et al., 2011, Ragab et al., 2011, Valanne et al., 2011, Ryu et al., 2010, Tanji et al., 2010, Ertürk-Hasdemir et al., 2009, Ha et al., 2009, Maillet et al., 2008, Kuttenkeuler and Boutros, 2007, Norgate et al., 2007, Quintin et al., 2007, Tsichritzis et al., 2007, Waterhouse et al., 2007, Akira et al., 2006, Gesellchen et al., 2005, Kwiatkowski, 2005, Paquette et al., 2005, Zhou et al., 2005, Silverman, 2003, Zhou et al., 2003)
    IMd
    (Tafesh-Edwards and Eleftherianos, 2020)
    Imd
    (Cai et al., 2021, Eleftherianos and Heryanto, 2021, Parra-Peralbo et al., 2021, Schlamp et al., 2021, Zhu et al., 2021, Alameh et al., 2020, Arora and Ligoxygakis, 2020, Dierking and Pita, 2020, Hanson and Lemaitre, 2020, Lin et al., 2020, Ozakman and Eleftherianos, 2020, Ramond et al., 2020, Tafesh-Edwards and Eleftherianos, 2020, Aalto et al., 2019, Maitra et al., 2019, Sanuki et al., 2019, Valanne et al., 2019, Min and Tatar, 2018, Peiris et al., 2018, Richardson and Portela, 2018, Zhai et al., 2018, Khadilkar et al., 2017, Kleino et al., 2017, Petkau et al., 2017, Hamilton et al., 2015, Kanoh et al., 2015, Woodcock et al., 2015, Zhan et al., 2015, Engel et al., 2014, Keebaugh and Schlenke, 2014, Myllymäki et al., 2014, Aparicio et al., 2013, Buchon et al., 2013, Katzenberger et al., 2013, Kuraishi et al., 2013, Petersen et al., 2013, Rancès et al., 2013, Eleftherianos and Castillo, 2012, Igboin et al., 2012, Kounatidis and Ligoxygakis, 2012, Chen et al., 2010, Junell et al., 2010, Kurata, 2010, Sabin et al., 2010, Bond and Foley, 2009, Jin et al., 2009, Thevenon et al., 2009, Bond et al., 2008, Goto et al., 2008, Kleino et al., 2008, Lhocine et al., 2008, Costa et al., 2007, Dijkers and O'Farrell, 2007, Koizumi et al., 2007, Kuranaga and Miura, 2007, Pal et al., 2007, Williams et al., 2007, Broderick et al., 2006, Steiner, 2004, Leulier et al., 2002, Kaisho and Akira, 2001, Vidal et al., 2001, Corbo, 1995.6.4)
    anon-WO0172774.166
    imd
    (Buhlman et al., 2021, Ferguson et al., 2021, Mishra et al., 2021, Pang et al., 2021, Tang et al., 2021, Yamashita et al., 2021, Cohen et al., 2020, Dziedziech et al., 2020, Gilbert et al., 2020, Krautz et al., 2020, Li et al., 2020, Palmer et al., 2020, Vincent et al., 2020, Araki et al., 2019, Chen et al., 2019, Chmiel et al., 2019, Kano and Yagi, 2019, La Marca et al., 2019, Palmer et al., 2019, Parvy et al., 2019, Sanchez Bosch et al., 2019, Troha and Buchon, 2019, Wang et al., 2019, Westfall et al., 2019, Badinloo et al., 2018, Goto et al., 2018, Harris et al., 2018, Hori et al., 2018, Lin et al., 2018, Schretter et al., 2018, Shahrestani et al., 2018, Westfall et al., 2018, Chen et al., 2017, Christesen et al., 2017, Keesey et al., 2017, Kenmoku et al., 2017, Kounatidis et al., 2017, Transgenic RNAi Project members, 2017-, Chatterjee et al., 2016, Costechareyre et al., 2016, Lamiable et al., 2016, Morris et al., 2016, Shiratsuchi et al., 2016, Arefin et al., 2015, Clemmons et al., 2015, Gene Disruption Project members, 2015-, Lim et al., 2015, Momiuchi et al., 2015, Sansone et al., 2015, Vedanayagam and Garrigan, 2015, Verma and Tapadia, 2015, Wu et al., 2015, Xia et al., 2015, Xiao et al., 2015, Chambers et al., 2014, Chtarbanova et al., 2014, Kim et al., 2014, Péan and Dionne, 2014, Taylor et al., 2014, Verma and Tapadia, 2014, Aparicio et al., 2013, Christofi and Apidianakis, 2013, Kwon et al., 2013, Nelson et al., 2013, Pukklay et al., 2013, Yagi et al., 2013, Bangi et al., 2012, Chinchore et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Lemaitre et al., 2012, Neyen et al., 2012, Taillebourg et al., 2012, Tsuzuki et al., 2012, Verma and Tapadia, 2012, Akhouayri et al., 2011, Galac and Lazzaro, 2011, Glittenberg et al., 2011, Hyrsl et al., 2011, Limmer et al., 2011, Marcu et al., 2011, Zhao et al., 2011, Chen et al., 2010, Hill-Burns and Clark, 2010, Kim et al., 2010, Kong et al., 2010, Kong et al., 2010, Paquette et al., 2010, Righi et al., 2010, Tang et al., 2010, Valanne et al., 2010, Wang et al., 2010, Avadhanula et al., 2009, Costa et al., 2009, Cronin et al., 2009, Ha et al., 2009, Markovic et al., 2009, Zsindely et al., 2009, Gordon et al., 2008, Jin et al., 2008, Lee and Edery, 2008, Ramsden et al., 2008, Sekiya et al., 2008, Tan et al., 2008, Vonkavaara et al., 2008, Yano et al., 2008, Busse et al., 2007, Buszczak et al., 2007, Ferrandon et al., 2007, Gregory et al., 2007, Hallem et al., 2007, Huh et al., 2007, Kurata, 2007, Lesch et al., 2007, Minidorff et al., 2007, Minidorff et al., 2007, Nehme et al., 2007, Pham et al., 2007, Sackton et al., 2007, Taylor and Kimbrell, 2007, Vonkavaara et al., 2007, Wagner and Roeder, 2007, Wu et al., 2007, Kaneko et al., 2006, Kim et al., 2006, Leulier et al., 2006, Liehl et al., 2006, Kim et al., 2005, Mace et al., 2005, Mace et al., 2005, McGettigan et al., 2005, Pal and Wu, 2005, Thoetkiattikul et al., 2005, Zerofsky et al., 2005, Lazzaro et al., 2004, Lau et al., 2003, Yajima et al., 2003, Naitza et al., 2002, Rutschmann et al., 2002, Tingvall et al., 2001)
    shadok
    (Ferrandon et al., 2001)
    Name Synonyms
    Immune deficiency
    (Lazzaro et al., 2004)
    Immunodeficiency
    (Corbo, 1995.6.4)
    immune deficiency
    (Badinloo et al., 2018, Trinder et al., 2017, Lemaitre et al., 2012, Taillebourg et al., 2012, Marcu et al., 2011, Kim et al., 2010, Nehme et al., 2007, Kim et al., 2006, Lazzaro et al., 2006, Rutschmann et al., 2002, Tingvall et al., 2001)
    immune deficient
    (Zerofsky et al., 2005)
    Secondary FlyBase IDs
    • FBgn0034344
    • FBgn0046434
    Datasets (8)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    Study result (8)
    Result
    Result Type
    Title
    scRNAseq_2020_Cattenoz_NI_seq_clustering
    Clustering analysis of hemocytes from non-infested third instar larvae
    scRNAseq_2020_Cattenoz_WI_seq_clustering
    Clustering analysis of hemocytes from wasp-infested third instar larvae
    scRNAseq_2020_Cho_NI72LG_seq_clustering
    Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
    scRNAseq_2020_Cho_NI96LG_seq_clustering
    Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
    scRNAseq_2020_Cho_NI120LG_seq_clustering
    Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
    scRNAseq_2020_Cho_WI96LG_seq_clustering
    Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
    scRNAseq_2020_Cho_NI96HL_seq_clustering
    Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
    scRNAseq_2020_Cho_NI120HL_seq_clustering
    Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
    References (441)