Nrx, NrxIV, nrx IV, neurexin, Dnrx
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
None of the polypeptides share 100% sequence identity.
1283 (aa); 150-155 (kD observed); 145 (kD predicted)
The C-terminal region interacts with coracle. Interacts with Patj in cis form. Forms a complex with Nrg and Cont.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nrx-IV using the Feature Mapper tool.
Both exon 3 and 4 of Nrx-IV are detected via RT-PCR in embryonic glial cells. In embryonic neurons expression of exon 4 is more intense than of exon 3.
Nrx-IV transcripts are differentially expressed in a tissue-specific manner. In late embryos and larvae, Nrx-IV-RA transcript ("exon 4" isoform) is preferentiatially expressed in neurons, while Nrx-IV-RB ("exon 3" isoform) is expressed in glia.
Nrx transcripts are detected on northern blots at all stages of development tested except third instar larvae and peak in 6-18hr embryos. Transcripts are localized by in situ hybridization to epithelial cells of ectodermal origin beginning at embryonic stage 11. This includes cells of the epidermis, tracheal system, pharynx, esophagus, proventriculus, hindgut, salivary glands, PNS and CNS. In the PNS, staining is restricted to the scolopales of the lateral chordotonal organs and is restricted to glial cells. All tissues that express Nrx in the embyro are characterized by the presence of pleated septate junctions (pSJs). Nrx expression is first detected ~1hr before pSJ formation.
Nrx-IV protein is expressed in a short apical stretch of the basolateral membrane of embryonic septate junctions in the embryonic hindgut.
Nrx-IV protein is detected in the glial cell membranes in the peripheral nerves, ensheathing the axons. In the chordotonal organs, it is detected in the scolopale and cap cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Nrx-IV in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Nrx CG6827
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
The amino-terminal 383 amino acids of cora (a functional domain that is both necessary and sufficient for proper septate junction localisation of cora) and the cytoplasmic domain of Nrx-IV interact directly.
Complementation groups l(3)68Fa, l(3)68Fb, l(3)68Fc, l(3)68Fd, l(3)68Fe, Nrx-IV, l(3)69Aa and l(3)69Ab isolated by Hoogwerf et al. (FBrf0048238) probably correspond to complementation groups l(3)68Fg, l(3)j2D3, l(3)68Fh, rols, l(3)68Fi, Nrx-IV, l(3)69Ah and l(3)69Ai isolated by Baumgartner at al, 1996, Cell 87: 1059--1068, but a one to one correspondence cannot be made as Hoogwerf mutations are apparently lost.
Nrx-IV is a transmembrane protein of the septate junction and is required in the formation of septate-junction septa and intercellular barriers.
Identified in a PCR screen for homologues of the vertebrate neurexin.