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Gene Dmel\Nrx-IV

General Information
Symbol	Dmel\Nrx-IV	Species	D. melanogaster
Name	Neurexin IV	Annotation symbol	CG6827
Feature type	protein_coding_gene	FlyBase ID	FBgn0013997
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	Nrx, nrx IV, NrxIV, Dnrx
Genomic Location
Chromosome (arm)	3L	Recombination map	3-
Cytogenetic map	68F6-68F7	Sequence location	3L:12,139,675..12,150,106 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Neurexin IV is referred to in FlyBase by the symbol Dmel\Nrx-IV (CG6827, FBgn0013997). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: transmembrane signaling receptor activity. There is experimental evidence that it is involved in the biological process: dorsal closure; nerve maturation; regulation of tube size, open tracheal system; establishment of glial blood-brain barrier; septate junction assembly; axon ensheathment. 34 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; adult segment; non-connected developing system; thoracic segment; dorsal trunk primordium; scolopidium; synapse; external compound sense organ; plasma membrane part. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Coagulation factor 5/8 C-terminal type domain; Concanavalin A-like lectin/glucanase; Concanavalin A-like lectin/glucanase, subgroup; Epidermal growth factor-like; Epidermal growth factor-like, type 3; Galactose-binding domain-like; Laminin G domain; Laminin G, subdomain 2; Neurexin/syndecan/glycophorin C. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-18 hour embryonic stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, larval salivary gland, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval midgut, adult hindgut, larval/adult fat body, adult salivary gland, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1639459_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Nrx-IV. Gene sequence location is 3L:12139675..12150106.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References Oshima and Fehon, 2011, J. Cell Sci. 124(16): 2861--2871 Alleles Nrx-IV[D56] Sequence features BKN22273 HFA11238 BKN50342 BKN50077
FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 68F6-68F7   Limits computationally determined from genome sequence between P{PZ}rols08232&P{lacW}l(3)j2D3j2D3 and P{PZ}l(3)0508805088
Experimentally Determined Cytological Location
Cytogenetic map Notes References 68F-68F     (Baumgartner, 1995.4.26) 68F-68F   (determined by in situ hybridisation)   (Baumgartner, 1995) 36D-36D   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000)
Experimentally Determined Recombination Data
Location	3- (Baumgartner, 1995.4.26)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nrx-IV for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nrx-IV-RA FBtr0075998  NM_079310   4900   1284 Nrx-IV-RB FBtr0075999  NM_168491   4900   1284
Additional Transcript Data & Comments
Reported size (kB)	5.0 (northern blot) (Baumgartner et al., 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nrx-IV-PA   FBpp0075730   145.5   1284   6.38     NP_524034   AAF49951 Nrx-IV-PB   FBpp0075731   145.1   1284   6.48     NP_729787   AAN12252
Additional Polypeptide Data & Comments
Reported size (kDa)	1283 (aa); 150-155 (kD observed); 145 (kD predicted) (Baumgartner et al., 1996)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Coagulation factor 5/8 C-terminal type domain (IPR000421) Epidermal growth factor-like, type 3 (IPR000742) Laminin G domain (IPR001791) Neurexin/syndecan/glycophorin C (IPR003585) Epidermal growth factor-like (IPR006210) Galactose-binding domain-like (IPR008979) Concanavalin A-like lectin/glucanase (IPR008985) Laminin G, subdomain 2 (IPR012680) Concanavalin A-like lectin/glucanase, subgroup (IPR013320)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF49951   AE014296 AAN12252   AI297728   AQ025125   AQ254643   BG637437   BI213268   BI482739   BT011472   BT120004   CK657427   CO178082   CO187005   CO188582   CO188664   CO190489   CO196039   CO196353   CO266182   CO269289   CO279398   CO282595   CO287851   CO292149   CO293386   CO297423   CO300689   CO300701   CO311124   CO312424   CO313593   CO316417   CO317038   CO323212   CO324165   CO326998   CO330522   CO332221   CO332675   CO333300   CO339970   CO340444   EC057443   EC062938   EC063225   EC064842   EC072226   EC073642   EC073941   EC075111   EC079266   EC084470   EC090324   EC196140   EC198949   EC199974   EC200704   EC202179   EC203192   EC204007   EC206745   EC210816   EC216710   EC220033   EC224940   EC230162   EC237245   EC241152   EC241344   EC245468   EC246989   EC249896   EC251095   EC255673   EC257044   GH947721   GH952013   GH960049   GH962090   GH968148   GH970798   GH973851   GH975974   GH979002   X86685 CAA60383
UniProtKB/Swiss-Prot	Q94887
UniProtKB/TrEMBL	Q8IQH0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 17   embryonic/larval esophagus   (Baumgartner et al., 1996) scolopidium   (Baumgartner et al., 1996) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Baumgartner et al., 1996) embryonic stage 11 -- 17   glial cell   (Baumgartner et al., 1996) embryonic/larval salivary gland   (Baumgartner et al., 1996) embryonic/larval hindgut   (Baumgartner et al., 1996) peripheral nervous system | restricted   (Baumgartner et al., 1996) central nervous system | restricted   (Baumgartner et al., 1996) epidermis   (Baumgartner et al., 1996) tracheal system   (Baumgartner et al., 1996) embryonic/larval pharynx   (Baumgartner et al., 1996) chordotonal organ   (Baumgartner et al., 1996) embryonic/larval proventriculus   (Baumgartner et al., 1996)
Additional Descriptive Data
	Nrx-IV transcripts are detected on northern blots at all stages of development tested except third instar larvae and peak in 6-18hr embryos. Transcripts are localized by in situ hybridization to epithelial cells of ectodermal origin beginning at embryonic stage 11. This includes cells of the epidermis, tracheal system, pharynx, esophagus, proventriculus, hindgut, salivary glands, PNS and CNS. In the PNS, staining is restricted to the scolopales of the lateral chordotonal organs and is restricted to glial cells. All tissues that express Nrx-IV in the embyro are characterized by the presence of pleated septate junctions (pSJs). Nrx-IV expression is first detected ~1hr before pSJ formation. (Baumgartner et al., 1996)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   lateral ectoderm   • basolateral plasma membrane (Kutchma et al., 2007) lateral ectoderm   • lateral plasma membrane (Kutchma et al., 2007)
Additional Descriptive Data
	Nrx-IV protein was detected on all pleated septate junctions documented. (Baumgartner et al., 1996)
Marker for
Subcellular Localization
CV Term	pleated septate junction (Baumgartner et al., 1996)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0013997    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0013997 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-18 hour embryonic stages, at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0013997 expression level Developmental Stage   Expression Level embryo 00-02hr    1197 embryo 02-04hr    896 embryo 04-06hr    2101 embryo 06-08hr    4479 embryo 08-10hr    5799 embryo 10-12hr    6446 embryo 12-14hr    9750 embryo 14-16hr    5958 embryo 16-18hr    4511 embryo 18-20hr    3076 embryo 20-22hr    1864 embryo 22-24hr    2461 larva L1    1922 larva L2    1402 larva L3 12hr old    1017 larva L3 puffstage 1-2    1327 larva L3 puffstage 3-6    2422 larva L3 puffstage 7-9    3348 white prepupae new    4051 white prepupae 12hr    3914 white prepupae 24hr    5623 pupae 2d postWPP    4376 pupae 3d postWPP    3379 pupae 4d postWPP    1326 adult male 01day    1332 adult male 05day    1248 adult male 30day    1224 adult female 01day    1038 adult female 05day    1187 adult female 30day    1042 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1197) embryo 02-04hr  (896) embryo 04-06hr  (2101) embryo 06-08hr  (4479) embryo 08-10hr  (5799) embryo 10-12hr  (6446) embryo 12-14hr  (9750) embryo 14-16hr  (5958) embryo 16-18hr  (4511) embryo 18-20hr  (3076) embryo 20-22hr  (1864) embryo 22-24hr  (2461) larva L1  (1922) larva L2  (1402) larva L3 12hr old  (1017) larva L3 puffstage 1-2  (1327) larva L3 puffstage 3-6  (2422) larva L3 puffstage 7-9  (3348) white prepupae new  (4051) white prepupae 12hr  (3914) white prepupae 24hr  (5623) pupae 2d postWPP  (4376) pupae 3d postWPP  (3379) pupae 4d postWPP  (1326) adult male 01day  (1332) adult male 05day  (1248) adult male 30day  (1224) adult female 01day  (1038) adult female 05day  (1187) adult female 30day  (1042) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1197 embryo 02-04hr    896 embryo 04-06hr  (2101) embryo 06-08hr  (4479) embryo 08-10hr  (5799) embryo 10-12hr  (6446) embryo 12-14hr  (9750) embryo 14-16hr  (5958) embryo 16-18hr  (4511) embryo 18-20hr  (3076) embryo 20-22hr  (1864) embryo 22-24hr  (2461) larva L1  (1922) larva L2  1402 larva L3 12hr old    1017 larva L3 puffstage 1-2    1327 larva L3 puffstage 3-6  (2422) larva L3 puffstage 7-9  (3348) white prepupae new  (4051) white prepupae 12hr  (3914) white prepupae 24hr  (5623) pupae 2d postWPP  (4376) pupae 3d postWPP  (3379) pupae 4d postWPP    1326 adult male 01day    1332 adult male 05day    1248 adult male 30day    1224 adult female 01day    1038 adult female 05day    1187 adult female 30day    1042 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1197 embryo 02-04hr    896 embryo 04-06hr    2101 embryo 06-08hr    4479 embryo 08-10hr    5799 embryo 10-12hr    6446 embryo 12-14hr    9750 embryo 14-16hr    5958 embryo 16-18hr    4511 embryo 18-20hr    3076 embryo 20-22hr    1864 embryo 22-24hr    2461 larva L1    1922 larva L2    1402 larva L3 12hr old    1017 larva L3 puffstage 1-2    1327 larva L3 puffstage 3-6    2422 larva L3 puffstage 7-9    3348 white prepupae new    4051 white prepupae 12hr    3914 white prepupae 24hr    5623 pupae 2d postWPP    4376 pupae 3d postWPP    3379 pupae 4d postWPP    1326 adult male 01day    1332 adult male 05day    1248 adult male 30day    1224 adult female 01day    1038 adult female 05day    1187 adult female 30day    1042 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1197 embryo 02-04hr    896 embryo 04-06hr    2101 embryo 06-08hr    4479 embryo 08-10hr    5799 embryo 10-12hr    6446 embryo 12-14hr    9750 embryo 14-16hr    5958 embryo 16-18hr    4511 embryo 18-20hr    3076 embryo 20-22hr    1864 embryo 22-24hr    2461 larva L1    1922 larva L2    1402 larva L3 12hr old    1017 larva L3 puffstage 1-2    1327 larva L3 puffstage 3-6    2422 larva L3 puffstage 7-9    3348 white prepupae new    4051 white prepupae 12hr    3914 white prepupae 24hr    5623 pupae 2d postWPP    4376 pupae 3d postWPP    3379 pupae 4d postWPP    1326 adult male 01day    1332 adult male 05day    1248 adult male 30day    1224 adult female 01day    1038 adult female 05day    1187 adult female 30day    1042 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0013997 expression level Developmental Stage   Expression Level embryo 00-02hr    1197 embryo 02-04hr    896 embryo 04-06hr    2101 embryo 06-08hr    4479 embryo 08-10hr    5799 embryo 10-12hr    6446 embryo 12-14hr    9750 embryo 14-16hr    5958 embryo 16-18hr    4511 embryo 18-20hr    3076 embryo 20-22hr    1864 embryo 22-24hr    2461 larva L1    1922 larva L2    1402 larva L3 12hr old    1017 larva L3 puffstage 1-2    1327 larva L3 puffstage 3-6    2422 larva L3 puffstage 7-9    3348 white prepupae new    4051 white prepupae 12hr    3914 white prepupae 24hr    5623 pupae 2d postWPP    4376 pupae 3d postWPP    3379 pupae 4d postWPP    1326 adult male 01day    1332 adult male 05day    1248 adult male 30day    1224 adult female 01day    1038 adult female 05day    1187 adult female 30day    1042 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1197) embryo 02-04hr  (896) embryo 04-06hr  (2101) embryo 06-08hr  (4479) embryo 08-10hr  (5799) embryo 10-12hr  (6446) embryo 12-14hr  (9750) embryo 14-16hr  (5958) embryo 16-18hr  (4511) embryo 18-20hr  (3076) embryo 20-22hr  (1864) embryo 22-24hr  (2461) larva L1  (1922) larva L2  (1402) larva L3 12hr old  (1017) larva L3 puffstage 1-2  (1327) larva L3 puffstage 3-6  (2422) larva L3 puffstage 7-9  (3348) white prepupae new  (4051) white prepupae 12hr  (3914) white prepupae 24hr  (5623) pupae 2d postWPP  (4376) pupae 3d postWPP  (3379) pupae 4d postWPP  (1326) adult male 01day  (1332) adult male 05day  (1248) adult male 30day  (1224) adult female 01day  (1038) adult female 05day  (1187) adult female 30day  (1042) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1197 embryo 02-04hr    896 embryo 04-06hr  2101 embryo 06-08hr  (4479) embryo 08-10hr  (5799) embryo 10-12hr  (6446) embryo 12-14hr  (9750) embryo 14-16hr  (5958) embryo 16-18hr  (4511) embryo 18-20hr  (3076) embryo 20-22hr  1864 embryo 22-24hr  2461 larva L1  1922 larva L2  1402 larva L3 12hr old    1017 larva L3 puffstage 1-2    1327 larva L3 puffstage 3-6  2422 larva L3 puffstage 7-9  (3348) white prepupae new  (4051) white prepupae 12hr  (3914) white prepupae 24hr  (5623) pupae 2d postWPP  (4376) pupae 3d postWPP  (3379) pupae 4d postWPP    1326 adult male 01day    1332 adult male 05day    1248 adult male 30day    1224 adult female 01day    1038 adult female 05day    1187 adult female 30day    1042 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1197 embryo 02-04hr    896 embryo 04-06hr    2101 embryo 06-08hr    4479 embryo 08-10hr    5799 embryo 10-12hr    6446 embryo 12-14hr    9750 embryo 14-16hr    5958 embryo 16-18hr    4511 embryo 18-20hr    3076 embryo 20-22hr    1864 embryo 22-24hr    2461 larva L1    1922 larva L2    1402 larva L3 12hr old    1017 larva L3 puffstage 1-2    1327 larva L3 puffstage 3-6    2422 larva L3 puffstage 7-9    3348 white prepupae new    4051 white prepupae 12hr    3914 white prepupae 24hr    5623 pupae 2d postWPP    4376 pupae 3d postWPP    3379 pupae 4d postWPP    1326 adult male 01day    1332 adult male 05day    1248 adult male 30day    1224 adult female 01day    1038 adult female 05day    1187 adult female 30day    1042 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1197 embryo 02-04hr    896 embryo 04-06hr    2101 embryo 06-08hr    4479 embryo 08-10hr    5799 embryo 10-12hr    6446 embryo 12-14hr    9750 embryo 14-16hr    5958 embryo 16-18hr    4511 embryo 18-20hr    3076 embryo 20-22hr    1864 embryo 22-24hr    2461 larva L1    1922 larva L2    1402 larva L3 12hr old    1017 larva L3 puffstage 1-2    1327 larva L3 puffstage 3-6    2422 larva L3 puffstage 7-9    3348 white prepupae new    4051 white prepupae 12hr    3914 white prepupae 24hr    5623 pupae 2d postWPP    4376 pupae 3d postWPP    3379 pupae 4d postWPP    1326 adult male 01day    1332 adult male 05day    1248 adult male 30day    1224 adult female 01day    1038 adult female 05day    1187 adult female 30day    1042 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0013997    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0013997 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, larval salivary gland, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval midgut, adult hindgut, larval/adult fat body, adult salivary gland, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0013997 expression level Tissue   Expression Level Larval Central Nervous System    907.2 Larval Midgut    115.7 Larval Hindgut    556.2 Larval Malpighian Tubules    61 Larval Fat Body    215.6 Larval Salivary Gland    716.6 Larval Trachea    538.975 Larval Carcass    371.05 Adult Head    273.9 Adult Eye    139.575 Adult Brain    317.7 Adult Thoracic-Abdominal Ganglion    431.8 Adult Crop    390.9 Adult Midgut    82.3 Adult Hindgut    248.8 Adult Malpighian Tubules    38.9 Adult Fat Body    112.2 Adult Salivary Gland    461.8 Adult Heart    77.1 Adult VirginFemale Spermatheca    170.2 Adult InseminatedFemale Spermatheca    247.9 Adult Ovary    229 Adult Testis    232.9 Adult Male Accessory Gland    439.5 Adult Carcass    171.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (907.2) Larval Midgut    115.7 Larval Hindgut  (556.2) Larval Malpighian Tubules    61 Larval Fat Body    215.6 Larval Salivary Gland  (716.6) Larval Trachea  538.975 Larval Carcass    371.05 Adult Head    273.9 Adult Eye    139.575 Adult Brain    317.7 Adult Thoracic-Abdominal Ganglion    431.8 Adult Crop    390.9 Adult Midgut    82.3 Adult Hindgut    248.8 Adult Malpighian Tubules    38.9 Adult Fat Body    112.2 Adult Salivary Gland    461.8 Adult Heart    77.1 Adult VirginFemale Spermatheca    170.2 Adult InseminatedFemale Spermatheca    247.9 Adult Ovary    229 Adult Testis    232.9 Adult Male Accessory Gland    439.5 Adult Carcass    171.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    907.2 Larval Midgut    115.7 Larval Hindgut    556.2 Larval Malpighian Tubules    61 Larval Fat Body    215.6 Larval Salivary Gland    716.6 Larval Trachea    538.975 Larval Carcass    371.05 Adult Head    273.9 Adult Eye    139.575 Adult Brain    317.7 Adult Thoracic-Abdominal Ganglion    431.8 Adult Crop    390.9 Adult Midgut    82.3 Adult Hindgut    248.8 Adult Malpighian Tubules    38.9 Adult Fat Body    112.2 Adult Salivary Gland    461.8 Adult Heart    77.1 Adult VirginFemale Spermatheca    170.2 Adult InseminatedFemale Spermatheca    247.9 Adult Ovary    229 Adult Testis    232.9 Adult Male Accessory Gland    439.5 Adult Carcass    171.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    907.2 Larval Midgut    115.7 Larval Hindgut    556.2 Larval Malpighian Tubules    61 Larval Fat Body    215.6 Larval Salivary Gland    716.6 Larval Trachea    538.975 Larval Carcass    371.05 Adult Head    273.9 Adult Eye    139.575 Adult Brain    317.7 Adult Thoracic-Abdominal Ganglion    431.8 Adult Crop    390.9 Adult Midgut    82.3 Adult Hindgut    248.8 Adult Malpighian Tubules    38.9 Adult Fat Body    112.2 Adult Salivary Gland    461.8 Adult Heart    77.1 Adult VirginFemale Spermatheca    170.2 Adult InseminatedFemale Spermatheca    247.9 Adult Ovary    229 Adult Testis    232.9 Adult Male Accessory Gland    439.5 Adult Carcass    171.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0013997 expression level Tissue   Expression Level Larval Central Nervous System    907.2 Larval Midgut    115.7 Larval Hindgut    556.2 Larval Malpighian Tubules    61 Larval Fat Body    215.6 Larval Salivary Gland    716.6 Larval Trachea    538.975 Larval Carcass    371.05 Adult Head    273.9 Adult Eye    139.575 Adult Brain    317.7 Adult Thoracic-Abdominal Ganglion    431.8 Adult Crop    390.9 Adult Midgut    82.3 Adult Hindgut    248.8 Adult Malpighian Tubules    38.9 Adult Fat Body    112.2 Adult Salivary Gland    461.8 Adult Heart    77.1 Adult VirginFemale Spermatheca    170.2 Adult InseminatedFemale Spermatheca    247.9 Adult Ovary    229 Adult Testis    232.9 Adult Male Accessory Gland    439.5 Adult Carcass    171.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (907.2) Larval Midgut    115.7 Larval Hindgut  556.2 Larval Malpighian Tubules    61 Larval Fat Body    215.6 Larval Salivary Gland  716.6 Larval Trachea  538.975 Larval Carcass    371.05 Adult Head    273.9 Adult Eye    139.575 Adult Brain    317.7 Adult Thoracic-Abdominal Ganglion    431.8 Adult Crop    390.9 Adult Midgut    82.3 Adult Hindgut    248.8 Adult Malpighian Tubules    38.9 Adult Fat Body    112.2 Adult Salivary Gland    461.8 Adult Heart    77.1 Adult VirginFemale Spermatheca    170.2 Adult InseminatedFemale Spermatheca    247.9 Adult Ovary    229 Adult Testis    232.9 Adult Male Accessory Gland    439.5 Adult Carcass    171.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    907.2 Larval Midgut    115.7 Larval Hindgut    556.2 Larval Malpighian Tubules    61 Larval Fat Body    215.6 Larval Salivary Gland    716.6 Larval Trachea    538.975 Larval Carcass    371.05 Adult Head    273.9 Adult Eye    139.575 Adult Brain    317.7 Adult Thoracic-Abdominal Ganglion    431.8 Adult Crop    390.9 Adult Midgut    82.3 Adult Hindgut    248.8 Adult Malpighian Tubules    38.9 Adult Fat Body    112.2 Adult Salivary Gland    461.8 Adult Heart    77.1 Adult VirginFemale Spermatheca    170.2 Adult InseminatedFemale Spermatheca    247.9 Adult Ovary    229 Adult Testis    232.9 Adult Male Accessory Gland    439.5 Adult Carcass    171.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    907.2 Larval Midgut    115.7 Larval Hindgut    556.2 Larval Malpighian Tubules    61 Larval Fat Body    215.6 Larval Salivary Gland    716.6 Larval Trachea    538.975 Larval Carcass    371.05 Adult Head    273.9 Adult Eye    139.575 Adult Brain    317.7 Adult Thoracic-Abdominal Ganglion    431.8 Adult Crop    390.9 Adult Midgut    82.3 Adult Hindgut    248.8 Adult Malpighian Tubules    38.9 Adult Fat Body    112.2 Adult Salivary Gland    461.8 Adult Heart    77.1 Adult VirginFemale Spermatheca    170.2 Adult InseminatedFemale Spermatheca    247.9 Adult Ovary    229 Adult Testis    232.9 Adult Male Accessory Gland    439.5 Adult Carcass    171.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    273.9   NA  Eye    139.575   NA  Brain    317.7   907.2  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    431.8   NA  Crop    390.9   115.7  Midgut    82.3   556.2  Hindgut    248.8   61  Malpighian Tubules    38.9   215.6  Fat Body    112.2   716.6  Salivary Gland    461.8   NA  Heart    77.1   538.975  Trachea    NA   NA  VirginFemale Spermatheca    170.2   NA  InseminatedFemale Spermatheca    247.9   NA  Ovary    229   NA  Testis    232.9   NA  Male Accessory Gland    439.5   371.05  Carcass    171.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_11
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013997 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6827-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 Nrx-IVGD2436 lethal | recessive Nrx-IV14 Nrx-IV46 Nrx-IV319 Nrx-IV575 Nrx-IV711 Nrx-IV1817 Nrx-IV2511 Nrx-IV4025 Nrx-IV4164 Nrx-IV4304 Nrx-IV4865 Nrx-IVD56 viable Nrx-IVCA06597 Other Phenotypes Allele decreased cell number | somatic clone | third instar larval stage Nrx-IVD56 neuroanatomy defective Nrx-IV4025 neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 Nrx-IVEP809 neurophysiology defective (with Df(3L)BK9) Nrx-IV46 Nrx-IV4304 paralytic Nrx-IV46 Nrx-IV4025 Nrx-IV4304 visible Nrx-IV14 Nrx-IV319 Nrx-IV575 Nrx-IV711 Nrx-IV2511 Phenotype manifest in Allele abdominal lateral pentascolopidial chordotonal organ lch5 Nrx-IV4304 anterior fascicle & synapse, with Scer\GAL4elav-C155 Nrx-IVEP809 anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155 Nrx-IVEP809 axolemma & nerve Nrx-IV4304 central nervous system Nrx-IV4304 dorsal trunk primordium (with Nrx-IV4025) Nrx-IV4865 dorsal trunk primordium (with Nrx-IV4865) Nrx-IV4025 embryonic/first instar larval cuticle | dorsal Nrx-IV4304 Nrx-IV46 embryonic/larval brain | somatic clone | third instar larval stage Nrx-IVD56 embryonic/larval salivary gland | embryonic stage Nrx-IV4304 Nrx-IV46 embryonic epidermis | dorsal Nrx-IV46 embryonic ganglionic branch & embryonic tracheole (with Nrx-IV4025) Nrx-IV4865 embryonic ganglionic branch & embryonic tracheole (with Nrx-IV4865) Nrx-IV4025 eye Nrx-IV14 Nrx-IV2511 Nrx-IV319 Nrx-IV575 Nrx-IV711 germline cell | male, with Scer\GAL4ptc-559.1 Nrx-IVEP809 intersegmental nerve, with Scer\GAL4elav-C155 Nrx-IVEP809 leg Nrx-IV14 Nrx-IV2511 Nrx-IV319 Nrx-IV575 Nrx-IV711 neuromuscular junction Nrx-IV4304 Nrx-IV46 pleated septate junction Nrx-IV4025 Nrx-IV4304 Nrx-IV46 presumptive embryonic/larval tracheal system Nrx-IV4304 scolopidium Nrx-IV4304 scolopidium & abdominal lateral pentascolopidial chordotonal organ lch5 Nrx-IV4304 septate junction & peripheral glial cell Nrx-IV4304 wing Nrx-IV14 Nrx-IV2511 Nrx-IV319 Nrx-IV575 Nrx-IV711 wing vein Nrx-IV14 Nrx-IV2511 Nrx-IV319 Nrx-IV575 Nrx-IV711
Classical Alleles ( 27 )
For All Classical Alleles Show
Allele of Nrx-IV Class Mutagen Stocks Known lesion Nrx-IV4304 amorphic allele - genetic evidence, hypomorphic allele - genetic evidence ethyl methanesulfonate 2 -- Nrx-IVEP604 P-element activity 1 -- Nrx-IVEY06647 P-element activity 1 -- Nrx-IVMB01471 1 -- Nrx-IVMB08273 1 -- Nrx-IV4025 amorphic allele - genetic evidence, hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nrx-IV4164 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nrx-IV46 amorphic allele - genetic evidence, hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nrx-IV4865 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nrx-IV14 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nrx-IV1817 ethyl methanesulfonate 0 -- Nrx-IV1 ethyl methanesulfonate 0 -- Nrx-IV2511 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nrx-IV2 ethyl methanesulfonate 0 -- Nrx-IV319 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nrx-IV3 ethyl methanesulfonate 0 -- Nrx-IV4846 0 -- Nrx-IV4 ethyl methanesulfonate 0 -- Nrx-IV575 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nrx-IV5 ethyl methanesulfonate 0 -- Nrx-IV6 ethyl methanesulfonate 0 -- Nrx-IV711 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nrx-IVCA06597 P-element activity 0 Yes Nrx-IVCPTI001977 piggyBac transposase 0 Yes Nrx-IVD56 ethyl methanesulfonate 0 -- Nrx-IVEP809 P-element activity 0 Yes Nrx-IVPTT 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nrx-IV Class Mutagen Stocks Known lesion Nrx-IVGD2436 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Nrx-IVHMS00419 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nrx-IVJF03142 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nrx-IVKK102207 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nrx-IV+tBa in vitro construct - genomic fragment 0 Yes Nrx-IVScer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nrx-IVtCH322-154P15 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)BK9 (Baumgartner et al., 1996) Df(3L)Exel6116 (Ryder, 2004.4.26) Df(3L)vin5 (Hoogwerf et al., 1988, Baumgartner et al., 1996) Df(3L)vin6 (Hoogwerf et al., 1988) Df(3L)vin7 (Hoogwerf et al., 1988) Df(3L)vin8 (Hoogwerf et al., 1988, Baumgartner et al., 1996) Df(3L)vin9 (Hoogwerf et al., 1988) Df(3L)vin10 (Hoogwerf et al., 1988)
Not disrupted in	Df(3L)P20 (Hoogwerf et al., 1988) Df(3L)vin2 (Hoogwerf et al., 1988) Df(3L)vin3 (Hoogwerf et al., 1988) Df(3L)vin4 (Hoogwerf et al., 1988, Baumgartner et al., 1996) Df(3L)vin11 (Hoogwerf et al., 1988) Df(3L)vin66 (Hoogwerf et al., 1988)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD2436} NA P{KK102207} NA P{TRiP.HMS00419} NA P{TRiP.JF03142} NA P{UAS-Nrx-IV.GFP} NA characterization construct P{nrx+} NA PBac{CH322-154P15} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Nrx-IVEP604 NA P{EP}Nrx-IVEP809 NA P{EPgy2}Nrx-IVEY06647 NA insertion of enhancer trap P{lacW}l(3)SH009SH009 No miscellaneous insertions P{PTT-GA}Nrx-IVCA06597 NA P{PTT-un1}Nrx-IVPTT NA PBac{681.P.FSVS-1}Nrx-IVCPTI001977 NA insertion of enhancer trap binary system Mi{ET1}Nrx-IVMB01471 No Mi{ET1}Nrx-IVMB08273 No
Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
axon ensheathment inferred from mutant phenotype (Banerjee et al., 2006) dorsal closure inferred from mutant phenotype (Schulte et al., 2003) establishment of glial blood-brain barrier inferred from mutant phenotype (Stork et al., 2008, Schwabe et al., 2005) nerve maturation inferred from mutant phenotype (Banerjee et al., 2006) regulation of tube size, open tracheal system inferred from mutant phenotype (Paul et al., 2003) septate junction assembly inferred from mutant phenotype (Banerjee et al., 2006)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Khanna et al., 2010) pleated septate junction inferred from direct assay (Faivre-Sarrailh et al., 2004)
Terms Based on Predictions or Assertions ( 14 terms )
Molecular Function
CV term Evidence References
transmembrane signaling receptor activity inferred from sequence or structural similarity with NCBI:NP_054860 (Mi et al., 2001.12.13)
Biological Process
CV term Evidence References
cell adhesion inferred from electronic annotation with InterPro:IPR000421 (FlyBase Curators et al., 2004-) cell-cell junction organization traceable author statement (Harden, 2002) dorsal closure non-traceable author statement (Harden, 2002) traceable author statement (Noselli and Agnes, 1999, Tepass et al., 2001) establishment of blood-nerve barrier non-traceable author statement (Swiss-Prot Project Members, 2002.3.1) establishment or maintenance of cell polarity non-traceable author statement (Humbert et al., 2003) protein localization traceable author statement (Noselli and Agnes, 1999, Cereijido et al., 2003) septate junction assembly non-traceable author statement (Swiss-Prot Project Members, 2002.3.1) synaptic vesicle docking involved in exocytosis non-traceable author statement (Lloyd et al., 2000) synaptic vesicle targeting non-traceable author statement (Lloyd et al., 2000)
Cellular Component
CV term Evidence References
integral to membrane non-traceable author statement (Swiss-Prot Project Members, 2002.3.1) integral to plasma membrane non-traceable author statement (Harden, 2002) plasma membrane inferred from sequence or structural similarity (Baumgartner et al., 1996) septate junction non-traceable author statement (Harden, 2002, Tepass et al., 2001) traceable author statement (Noselli and Agnes, 1999, Cereijido et al., 2003, Medina et al., 2002, Humbert et al., 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nrx-IV allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64741 DroID - A comprehensive database of gene and protein interactions. • FBgn0013997 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39387
Orthologs
Genome-wide drosophilid orthologs	Dana\GF24804 Dere\GG15562 Dgri\GH16153 Dmoj\GI12425 Dper\GL25157 Dpse\GA19887 Dsec\GM25331 Dsim\GD14363 Dvir\GJ12321 Dwil\GK25462 Dyak\GE21889
Curated drosophilid orthologs	Dpse\GA19887
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL005321-PA Aedes aegypti (mosquito) AGAP007545-PA Anopheles gambiae (mosquito) XP_624195.2 Apis mellifera (honey bee) 282496 Branchiostoma floridae (lancelet) BGIBMGA002547-PA Bombyx mori (silk worm) ENSBTAP00000034672 Bos taurus (bovine) CBP26226 Caenorhabditis briggsae (nematode) CE40647 Caenorhabditis elegans (worm) ENSCAFP00000006992 Canis familiaris (dog) JA15274 Caenorhabditis japonica (nematode) CPIJ011417 Culex pipens (mosquito) ENSCPOP00000005977 Cavia porcellus (guinea pig) RP34860 Caenorhabditis remanei (nematode) 152031 Capitella spl (polychaete worm) 301922 Daphnia pulex (daphnia) ENSECAP00000010589 Equus caballus (horse) ENSGACP00000019239 Gymnopilus aculeatus (mushroom) ENSGALP00000018862 Gallus gallus (chicken) 192677 Hypsipyla robusta (cedar shoot borer) ENSP00000354778 Homo sapiens (human) ISCW021414-PA Ixodes scapularis (deer tick) 118711 Lottia gigantea (owl limpet) ENSMODP00000000784 Monodelphis domestica (gray opossum) ENSMMUP00000005226 Macaca mulatta (rhesus monkey) ENSMUSP00000110288 Mus musculus (house mouse) 240263 Nematostella vectensis (starlet anemone) XP_001604581.1 Nasonia vitripennis (wasp) ENSOANP00000012929 Ornithorynchus anatinus (platypus) ENSORLP00000005535 Oryzias latipes (japanese killifish) PHUM008070-PA Pediculus humanus (human body louse) PP00060 Pristionchus pacificus (nematode) ENSPPYP00000020350 Pongo pygmaeus (orangutan) ENSPTRP00000046704 Pan troglodytes (chimpanzee) ENSRNOP00000008688 Rattus norvegicus (norway rat) XP_780725.2 Strongylocentrotus purpuratus (purple sea urchin) 60571 Trichoplax adhaerens (metazoa) XP_972512.2 Tribolium castaneum (red flour beetle) ENSTNIP00000000286 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000023415 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000023787 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13304 LH12109 ISCW024754 ISCW023368 FBgn0239131 FBgn0227421 FBgn0199567 FBgn0186048 FBgn0180191 JGI_V11_301922 FBgn0079883 FBgn0162744 FBgn0135182 FBgn0123624 FBgn0107810 FBgn0101797 CPIJ011417 BGIBMGA002547 GB14382 AGAP007545 AAEL005321 PB21000 PHUM259620 GLEAN_11252
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	28715 y1 v1; P{TRiP.JF03142}attP2 23319 y1 w67c23; Mi{ET1}Nrx-IVMB01471 17185 w1118; P{EP}Nrx-IVEP604/TM3, Sb1 32424 y1 sc* v1; P{TRiP.HMS00419}attP2/TM3, Sb1 25595 w1118; Mi{ET1}Nrx-IVMB08273 eapMB08273/TM6C, Sb1 15966 y1 w67c23; P{EPgy2}Nrx-IVEY06647/TM3, Sb1 Ser1 4380 ru1 h1 Nrx-IV4304 st1 ry506 e1/TM6B, Tb+
Kyoto	107714 ru1 h1 Nrx-IV4304 st1 ry506 e1/TM6B, Tb1
VDRC	v108128 P{KK102207}VIE-260B v8353 w1118; P{GD2436}v8353 v9039 w1118; P{GD2436}v9039
Genomic Clones ( 2 )
	BACR05P17 BACR14K14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 79 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE18634
Other clones	FI12817
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP12093 LP23712 RE65477 SD15441
Other clones	20998 33861 35176 42976 45181 47001 54242 54244 60689 64191 64728 71488 72127 83474 100962 101972 107732 122302 161245 162616 192303 214217.45 214503.42 274911 276957 281429 CK01.26C.E6 CK01.49A.A8 CK01.49B.F7 CK01.59D.E8 CK01.69A.A8 CK01.84A.E2 CK01.123C.C2 EK000305 EK003320 EK025239 EK041036 EK041424 EK048352 EK055236 EK066949 EK094041 EK134232 EK145235 EK160810 EK179122 EK179210 EK189947 EK192857 EK199328 EK202815 EK216939 EK223436 EK228728 EK233519 EK264911 EK266760 EK295460 EK302830 EK304213 EK306430 EP05426 EP09801 EP10653 RP004149931 RP004207240 RP004255569 RP004330442 RP004374609 RP004421815 RP004459039 RP004530561 RP004549862
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013997 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39387
Antibody Information
	polyclonal (Baumgartner et al., 1996)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Nrx CG6827 (Zhang, 1999.11)
Source for database merge of
Additional comments
Other Comments
	Proper targetting of pck gene product to the pleated septate junctions in the tracheal system depends on Nrx-IV and cora but not Fas3. (Behr et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) The amino-terminal 383 amino acids of cora (a functional domain that is both necessary and sufficient for proper septate junction localisation of cora) and the cytoplasmic domain of Nrx-IV interact directly. (Ward et al., 1998) Complementation groups l(3)68Fa, l(3)68Fb, l(3)68Fc, l(3)68Fd, l(3)68Fe, Nrx-IV, l(3)69Aa and l(3)69Ab isolated by Hoogwerf et al. (FBrf0048238) probably correspond to complementation groups l(3)68Fg, l(3)j2D3, l(3)68Fh, rols, l(3)68Fi, Nrx-IV, l(3)69Ah and l(3)69Ai isolated by Baumgartner at al, 1996, Cell 87: 1059--1068, but a one to one correspondence cannot be made as Hoogwerf mutations are apparently lost. (Bellen, 1997.1.14) P{nrx+} transformant chromosomes fully rescue the embryonic lethality of l(3)68Ff mutations, demonstrating l(3)68Ff and Nrx-IV are the same complementation group. (Baumgartner et al., 1996) Nrx-IV is a transmembrane protein of the septate junction and is required in the formation of septate-junction septa and intercellular barriers. (Baumgartner et al., 1996) PCR-primers from the EGF-like region of chicken tenascin have been used to amplify Nrx-IV, Nrx-IV shows high homology to the EGF domain. (Baumgartner et al., 1995) Nrx-IV has a transcript of 5.0 kb, and encodes a protein of 1283 aa. The protein has an apparent molecular weight of about 155kD, with a domain structure almost identical to vertebrate neurexin. (Baumgartner, 1995.6.22) Identified in a PCR screen for homologues of the vertebrate neurexin. (Baumgartner et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079310 NM_168491 RefSeq (Proteins) • NP_524034 NP_729787 DDBJ / EMBL / GenBank • AAF49951 AAN12252 AQ025125 AQ254643 BI213268 CAA60383 X86685 Entrez Gene - A searchable database of RefSeq genes. • 39387 UniProtKB/Swiss-Prot • Q94887 UniProtKB/TrEMBL • Q8IQH0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Coagulation factor 5/8 C-terminal type domain (IPR000421) Epidermal growth factor-like, type 3 (IPR000742) Laminin G domain (IPR001791) Neurexin/syndecan/glycophorin C (IPR003585) Epidermal growth factor-like (IPR006210) Galactose-binding domain-like (IPR008979) Concanavalin A-like lectin/glucanase (IPR008985) Laminin G, subdomain 2 (IPR012680) Concanavalin A-like lectin/glucanase, subgroup (IPR013320)
Linkouts
	BioGRID - A database of protein and genetic interactions • 64741 DroID - A comprehensive database of gene and protein interactions. • FBgn0013997 DRSC - Results from RNAi screens. • FBgn0013997 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013997 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6827-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013997 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39387 InParanoid A subset of ortholog calls from InParanoid. • AAEL005321-PA Aedes aegypti (mosquito) AGAP007545-PA Anopheles gambiae (mosquito) XP_624195.2 Apis mellifera (honey bee) 282496 Branchiostoma floridae (lancelet) BGIBMGA002547-PA Bombyx mori (silk worm) ENSBTAP00000034672 Bos taurus (bovine) CBP26226 Caenorhabditis briggsae (nematode) CE40647 Caenorhabditis elegans (worm) ENSCAFP00000006992 Canis familiaris (dog) JA15274 Caenorhabditis japonica (nematode) CPIJ011417 Culex pipens (mosquito) ENSCPOP00000005977 Cavia porcellus (guinea pig) RP34860 Caenorhabditis remanei (nematode) 152031 Capitella spl (polychaete worm) 301922 Daphnia pulex (daphnia) ENSECAP00000010589 Equus caballus (horse) ENSGACP00000019239 Gymnopilus aculeatus (mushroom) ENSGALP00000018862 Gallus gallus (chicken) 192677 Hypsipyla robusta (cedar shoot borer) ENSP00000354778 Homo sapiens (human) ISCW021414-PA Ixodes scapularis (deer tick) 118711 Lottia gigantea (owl limpet) ENSMODP00000000784 Monodelphis domestica (gray opossum) ENSMMUP00000005226 Macaca mulatta (rhesus monkey) ENSMUSP00000110288 Mus musculus (house mouse) 240263 Nematostella vectensis (starlet anemone) XP_001604581.1 Nasonia vitripennis (wasp) ENSOANP00000012929 Ornithorynchus anatinus (platypus) ENSORLP00000005535 Oryzias latipes (japanese killifish) PHUM008070-PA Pediculus humanus (human body louse) PP00060 Pristionchus pacificus (nematode) ENSPPYP00000020350 Pongo pygmaeus (orangutan) ENSPTRP00000046704 Pan troglodytes (chimpanzee) ENSRNOP00000008688 Rattus norvegicus (norway rat) XP_780725.2 Strongylocentrotus purpuratus (purple sea urchin) 60571 Trichoplax adhaerens (metazoa) XP_972512.2 Tribolium castaneum (red flour beetle) ENSTNIP00000000286 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000023415 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000023787 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/neurexn1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39387 modMine - Data generated by the modENCODE project. • FBgn0013997 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13304 LH12109 ISCW024754 ISCW023368 FBgn0239131 FBgn0227421 FBgn0199567 FBgn0186048 FBgn0180191 JGI_V11_301922 FBgn0079883 FBgn0162744 FBgn0135182 FBgn0123624 FBgn0107810 FBgn0101797 CPIJ011417 BGIBMGA002547 GB14382 AGAP007545 AAEL005321 PB21000 PHUM259620 GLEAN_11252
Synonyms & Secondary IDs ( 27 )
Reported As
Symbol Synonym	CG6827 (Kraut et al., 2001, Kraut et al., 2001, Keleman et al., 2009.8.5, Perkins et al., 2009, Khanna et al., 2010, Ni et al., 2010.12.1) CT21123 (Hynes and Zhao, 2000) Dnrx (Yuan and Ganetzky, 1998, Littleton et al., 1995) l(3)68Ff   l(3)rsg23   Nrx (Ng et al., 2006, Genova and Fehon, 2003, Behr et al., 2003, Schulte et al., 2003, Wu and Beitel, 2004, Dudu et al., 2004, Wu et al., 2005, Jasper et al., 2002, Zhang et al., 2000, Whitfield et al., 2002, Wayne and McIntyre, 2002, Ward and Fehon, 1997, Trapp and Kidd, 2000, Schulz et al., 2004, Muller, 2000, Magie et al., 2002, Liu and Lengyel, 2000, Lamb et al., 1998, Hoover and Bryant, 2000, Harden, 2002, Goberdhan and Wilson, 1998, Fuss et al., 2001, Bilder, 2001, Bilder, 2001, Bilder and Perrimon, 2000, Baumgartner et al., 1995, Llimargas et al., 2004, Boettner et al., 2003, Tanentzapf and Tepass, 2003, Lloyd et al., 2000, Ward et al., 1998, Bryant, 1997, Ward and Fehon, 1997, Bellen, 1997.1.14, Baumgartner, 1995.6.22, Baumgartner et al., 1995, Baumgartner, 1995, Furuse and Tsukita, 2006, Schwabe et al., 2005, Wang et al., 2006, Zeitler et al., 2004, Schneider et al., 2006, Strigini et al., 2006, Schotman et al., 2008, Gorfinkiel and Arias, 2007, Alves-Silva et al., 2008, Nelson et al., 2010) NRX (Genova and Fehon, 2003, Mueller and Bossinger, 2003) nrx (Wu et al., 2004, Cereijido et al., 2003, Paul et al., 2003, Baumgartner, 1995.4.26, Agnes and Noselli, 1999, Noselli, 1998, Bhat et al., 1997, Bhat et al., 1997, Baumgartner et al., 1996, Baumgartner et al., 1995, Tonning et al., 2005, Wu et al., 2007, Edenfeld et al., 2006, Moyer and Jacobs, 2008, Stork et al., 2009) nrx IV (Bhat, 2003, Faivre-Sarrailh et al., 2001, Jones, 2001, Littleton, 2000, Andrew et al., 2000, Noselli and Agnes, 1999, Laval et al., 2008) NrxIV (Medina et al., 2002, Bhat et al., 2000, Yeaman et al., 1999, Peles et al., 1997, Schneider et al., 2006, Gorfinkiel and Arias, 2007, Chung et al., 2009, Padash-Barmchi et al., 2010, Hatan et al., 2011, Watson et al., 2011) Nrx IV (Hemphala et al., 2003, Bellen et al., 1998, Bhat et al., 1998, Banerjee et al., 2006, Banerjee et al., 2008, Laval et al., 2008) nrxIV (Salinas et al., 2001, Stork et al., 2008, Stork et al., 2009) NRX IV (Yuan and Ganetzky, 1999, Bhat et al., 1999, Bhat et al., 1999, Littlejohn et al., 1997) Nrx-IV (Buszczak et al., 2007, Arancio et al., 2010, Yi et al., 2008, Wheeler et al., 2009, Moyer and Jacobs, 2008, Stork et al., 2009, Hijazi et al., 2009, Laprise et al., 2009, Venken et al., 2009, Laprise et al., 2010, Nilton et al., 2010) nrx-IV (Moyer and Jacobs, 2008) Nx IV (Missler and Sudhof, 1998) rsg23 (Hoogwerf et al., 1988)
Name Synonym	neurexin (Bryant, 1997, Baumgartner et al., 1996, Slack et al., 2006) Neurexin (Ward and Fehon, 1997, ) Neurexin-4 (Humbert et al., 2003) Neurexin 4 (Khanna et al., 2010) Neurexin IV (Ng et al., 2006, Bryant and Huwe, 2000, Nelson, 2003, Venema and Auld, 2003, Venema and Auld, 2003, Cantera et al., 2002, Fusse and Hoch, 2002, Baumgartner et al., 1998, Tepass, 1997, Bellen et al., 1998, Daneman and Barres, 2005, Zeitler et al., 2004, Tiklová et al., 2007, Wang et al., 2006, Schotman et al., 2008, Banerjee et al., 2008, Laval et al., 2008, Schulte et al., 2006, Alves-Silva et al., 2008, Stork et al., 2009, Hijazi et al., 2009, Laprise et al., 2009, Jeon and Zinn, 2009, Chung et al., 2009, Khanna et al., 2010, Nilton et al., 2010, Kim et al., 2010) neurexin IV (Tabuchi and Sudhof, 2002, Brophy, 2001, Missler et al., 1998, Bhat, 2003, Paul et al., 2003, Andrew et al., 2000, Kurusu et al., 2008, Gorfinkiel and Arias, 2007) NeurexinIV (Silies et al., 2007, Moyer and Jacobs, 2008, Nelson et al., 2010, Syed et al., 2011) Neurexin-IV (Yi et al., 2008) neurexinIV (Edenfeld et al., 2006, Stork et al., 2008) rose-gespleten region interval 23 (Hoogwerf et al., 1988)
Secondary FlyBase IDs
	FBgn0002261
References ( 172 )
Generate a list of	All references relating to this gene ( 172 )
List References by type	Research paper  ( 75 ) Supplementary material  ( 8 ) Review  ( 34 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 29 ) FlyBase analysis  ( 1 ) Letter  ( 2 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 11 )
	Hatan et al., 2011, J. Cell Biol. 192(2): 307--319 The Drosophila blood brain barrier is maintained by GPCR-dependent dynamic actin structures. [FBrf0212807] Oshima and Fehon, 2011, J. Cell Sci. 124(16): 2861--2871 Analysis of protein dynamics within the septate junction reveals a highly stable core protein complex that does not include the basolateral polarity protein Discs large. [FBrf0214577] Syed et al., 2011, J. Neurosci. 31(21): 7876--7885 The CD59 Family Member Leaky/Coiled Is Required for the Establishment of the Blood-Brain Barrier in Drosophila. [FBrf0213741] Watson et al., 2011, Development 138(7): 1285--1295 Drosophila hedgehog signaling and engrailed-runt mutual repression direct midline glia to alternative ensheathing and non-ensheathing fates. [FBrf0213237] Arancio et al., 2010, Genetics 185(1): 129--140 The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors. [FBrf0213976] Khanna et al., 2010, BMC Genomics 11(1): 302 Towards a membrane proteome in Drosophila: a method for the isolation of plasma membrane. [FBrf0210923] Kim et al., 2010, Mol. Cells 29(1): 93--98 Blood-brain barrier defects associated with Rbp9 mutation. [FBrf0210543] Laprise et al., 2010, Curr. Biol. 20(1): 55--61 Epithelial Polarity Proteins Regulate Drosophila Tracheal Tube Size in Parallel to the Luminal Matrix Pathway. [FBrf0209969] Nelson et al., 2010, Genetics 185(3): 831--839 The Drosophila Claudin Kune-kune Is Required for Septate Junction Organization and Tracheal Tube Size Control. [FBrf0211332] Nilton et al., 2010, Development 137(14): 2427--2437 Crooked, coiled and crimpled are three Ly6-like proteins required for proper localization of septate junction components. [FBrf0211147] Padash-Barmchi et al., 2010, J. Cell Sci. 123(23): 4052--4062 Control of Gliotactin localization and levels by tyrosine phosphorylation and endocytosis is necessary for survival of polarized epithelia. [FBrf0212409] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...