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Gene Dmel\Nsf2

General Information
Symbol	Dmel\Nsf2	Species	D. melanogaster
Name	NEM-sensitive fusion protein 2	Annotation symbol	CG33101
Feature type	protein_coding_gene	FlyBase ID	FBgn0013998
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	dNSF2, dNSF-2, CG9931, Nsf-2
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	87F15-87F15	Sequence location	3R:9,660,195..9,663,708 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene NEM-sensitive fusion protein 2 is referred to in FlyBase by the symbol Dmel\Nsf2 (CG33101, FBgn0013998). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: nucleoside-triphosphatase activity; ATP binding. There is experimental evidence that it is involved in the biological process: neuromuscular synaptic transmission; regulation of synaptic growth at neuromuscular junction. 17 alleles are reported. The phenotypes of these alleles are annotated with: wing margin; eye; trichogen cell; embryonic/larval neuromuscular junction; mesothoracic tergum; synaptic vesicle. It has one annotated transcript and one annotated polypeptide. Protein features are: AAA+ ATPase domain; ATPase, AAA-type, conserved site; ATPase, AAA-type, core; Aspartate decarboxylase-like domain; CDC48, N-terminal subdomain; CDC48, domain 2. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1639023_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Nsf2. Gene sequence location is 3R:9660195..9663708.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87F15-87F15   Limits computationally determined from genome sequence between P{PZ}flfl01949 and P{lacW}trxj14A6&P{PZ}trx00347
Experimentally Determined Cytological Location
Cytogenetic map Notes References 87F12-88A3     (Trimble, 1995.6.8) 87F-87F   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000)
Experimentally Determined Recombination Data
Location	3- (Trimble, 1995.6.8)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nsf2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nsf2-RA FBtr0082883  NM_176499   2540   752
Additional Transcript Data & Comments
Reported size (kB)	3.4, 3.0, 2.8, 2.4 (northern blot) (Pallanck et al., 1995) 2.4 (northern blot) (Boulianne and Trimble, 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nsf2-PA   FBpp0082346   83.4   752   6.71     NP_788676   AAF54995
Additional Polypeptide Data & Comments
Reported size (kDa)	752 (aa) (Pallanck et al., 1995) 744 (aa) (Boulianne and Trimble, 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • CDC48, N-terminal subdomain (IPR003338) AAA+ ATPase domain (IPR003593) ATPase, AAA-type, core (IPR003959) ATPase, AAA-type, conserved site (IPR003960) CDC48, domain 2 (IPR004201) Aspartate decarboxylase-like domain (IPR009010) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392496   AA440279   AA540014   AA698578   AA698904   AA951209   AE014297 AAF54995   AI109834   BE975266   BF491742   BF498981   BI164019   BI166577   BI212528   BI212540   BI213072   BI213531   BI229700   BI230194   BI354200   BI355719   BI374309   BI576916   BI586893   BI630136   BP543811   BP543812   BT010259   BT023784   CO154277   CO188422   CO278157   CO280530   CO312521   CO322407   EC060706   EC061836   EC087042   EC087881   EC198279   EC234997   EC260956   GH748946   GH750052   GH750113   GH751549   GH751683   GH756631   GH756895   GH758673   GH759874   GH761156   GH761219   GH772206   GH772485   GH773557   GH778852   GH780107   GH781351   GH787494   GH788086   GH789793   GH794413   GH795215   GH796644   GH797758   GH798210   GH800551   GH802201   GH804558   GH804586   GH805285   GH805702   GH807178   GH808252   GH810722   GH812714   GH813066   GH814461   GH816095   GH817321   GH817584   GH817987   GH819125   GH820624   GH821625   GH824041   GH824616   GH826927   GH827064   GH827811   GH833497   GH834779   GH836961   GH837788   GH838320   GH840946   GH842510   GH847302   GH848398   GH850103   GH852088   GH856206   GH858405   GH858692   GH859504   GH860988   GH861689   GH862766   GH866377   GH867094   GH867119   GH870728   GH873866   GH874502   GH884967   GH885557   GH885672   GH885950   GH886426   GH886429   GH887403   GH887486   GH887566   GH887817   GH888009   GH892597   GH893328   GH899234   GH901127   GH902151   GH904472   GH905125   GH905462   GH905646   GH908371   GH911458   GH913458   GH916119   GH916372   GH916749   GH920085   GH921944   GH922644   GH922675   GH925094   GH932319   GH933008   GH933826   GH933936   GH934683   GH937059   GH937156   GH938406   U28836 AAC46844   U30502 AAA75044
UniProtKB/Swiss-Prot	P54351
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic/larval midgut   (Boulianne and Trimble, 1995) embryonic/larval hindgut   (Boulianne and Trimble, 1995) embryonic stage 5 -- 6   organism | ubiquitous   (Boulianne and Trimble, 1995) embryonic stage 8 -- 10   posterior midgut primordium   (Boulianne and Trimble, 1995) anterior midgut primordium   (Boulianne and Trimble, 1995) embryonic stage 8 -- 11   mesoderm   (Boulianne and Trimble, 1995) ectoderm   (Boulianne and Trimble, 1995) embryonic stage 14 -- 17   presumptive embryonic/larval central nervous system   (Boulianne and Trimble, 1995) larval stage   embryonic/larval salivary gland | restricted   (Boulianne and Trimble, 1995) eye-antennal disc   (Boulianne and Trimble, 1995) ring gland   (Boulianne and Trimble, 1995) wing disc   (Boulianne and Trimble, 1995) ventral thoracic disc   (Boulianne and Trimble, 1995) imaginal disc   (Boulianne and Trimble, 1995) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage & larval stage & adult stage     (Pallanck et al., 1995)
Additional Descriptive Data
	Nsf2 transcripts are expressed at substantially higher levels in adults and larvae than in embryos. (Pallanck et al., 1995) The highest levels of Nsf2 transcripts are seen before and during cellularization. After gastrulation, expression is seen primarily in regions which give rise to ectodermal and endodermal tissues such as the midgut and the hindgut. Later, expression is also seen in the CNS. In larvae, Nsf2 expression is observed in the ring gland and in a restricted number of cells in the salivary gland. Expression is also observed in unusual patterns in the imaginal discs with the highest levels in the leg, wing and antennal discs. (Boulianne and Trimble, 1995)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
colocalizes_with SNARE complex inferred from direct assay (Guruharsha et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    89.05 Larval Midgut    102.4 Larval Hindgut    128.2 Larval Malpighian Tubules    208.7 Larval Fat Body    86 Larval Salivary Gland    162.7 Larval Trachea    111.05 Larval Carcass    122.025 Adult Head    77.1 Adult Eye    88.775 Adult Brain    69.9 Adult Thoracic-Abdominal Ganglion    58.1 Adult Crop    123.3 Adult Midgut    75.1 Adult Hindgut    135.8 Adult Malpighian Tubules    149.5 Adult Fat Body    148.8 Adult Salivary Gland    225.4 Adult Heart    218.175 Adult VirginFemale Spermatheca    67.1 Adult InseminatedFemale Spermatheca    89 Adult Ovary    111.9 Adult Testis    67.4 Adult Male Accessory Gland    109 Adult Carcass    109.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    89.05 Larval Midgut    102.4 Larval Hindgut    128.2 Larval Malpighian Tubules    208.7 Larval Fat Body    86 Larval Salivary Gland    162.7 Larval Trachea    111.05 Larval Carcass    122.025 Adult Head    77.1 Adult Eye    88.775 Adult Brain    69.9 Adult Thoracic-Abdominal Ganglion    58.1 Adult Crop    123.3 Adult Midgut    75.1 Adult Hindgut    135.8 Adult Malpighian Tubules    149.5 Adult Fat Body    148.8 Adult Salivary Gland    225.4 Adult Heart    218.175 Adult VirginFemale Spermatheca    67.1 Adult InseminatedFemale Spermatheca    89 Adult Ovary    111.9 Adult Testis    67.4 Adult Male Accessory Gland    109 Adult Carcass    109.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    89.05 Larval Midgut    102.4 Larval Hindgut    128.2 Larval Malpighian Tubules    208.7 Larval Fat Body    86 Larval Salivary Gland    162.7 Larval Trachea    111.05 Larval Carcass    122.025 Adult Head    77.1 Adult Eye    88.775 Adult Brain    69.9 Adult Thoracic-Abdominal Ganglion    58.1 Adult Crop    123.3 Adult Midgut    75.1 Adult Hindgut    135.8 Adult Malpighian Tubules    149.5 Adult Fat Body    148.8 Adult Salivary Gland    225.4 Adult Heart    218.175 Adult VirginFemale Spermatheca    67.1 Adult InseminatedFemale Spermatheca    89 Adult Ovary    111.9 Adult Testis    67.4 Adult Male Accessory Gland    109 Adult Carcass    109.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    89.05 Larval Midgut    102.4 Larval Hindgut    128.2 Larval Malpighian Tubules    208.7 Larval Fat Body    86 Larval Salivary Gland    162.7 Larval Trachea    111.05 Larval Carcass    122.025 Adult Head    77.1 Adult Eye    88.775 Adult Brain    69.9 Adult Thoracic-Abdominal Ganglion    58.1 Adult Crop    123.3 Adult Midgut    75.1 Adult Hindgut    135.8 Adult Malpighian Tubules    149.5 Adult Fat Body    148.8 Adult Salivary Gland    225.4 Adult Heart    218.175 Adult VirginFemale Spermatheca    67.1 Adult InseminatedFemale Spermatheca    89 Adult Ovary    111.9 Adult Testis    67.4 Adult Male Accessory Gland    109 Adult Carcass    109.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    89.05 Larval Midgut    102.4 Larval Hindgut    128.2 Larval Malpighian Tubules    208.7 Larval Fat Body    86 Larval Salivary Gland    162.7 Larval Trachea    111.05 Larval Carcass    122.025 Adult Head    77.1 Adult Eye    88.775 Adult Brain    69.9 Adult Thoracic-Abdominal Ganglion    58.1 Adult Crop    123.3 Adult Midgut    75.1 Adult Hindgut    135.8 Adult Malpighian Tubules    149.5 Adult Fat Body    148.8 Adult Salivary Gland    225.4 Adult Heart    218.175 Adult VirginFemale Spermatheca    67.1 Adult InseminatedFemale Spermatheca    89 Adult Ovary    111.9 Adult Testis    67.4 Adult Male Accessory Gland    109 Adult Carcass    109.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    89.05 Larval Midgut    102.4 Larval Hindgut    128.2 Larval Malpighian Tubules    208.7 Larval Fat Body    86 Larval Salivary Gland    162.7 Larval Trachea    111.05 Larval Carcass    122.025 Adult Head    77.1 Adult Eye    88.775 Adult Brain    69.9 Adult Thoracic-Abdominal Ganglion    58.1 Adult Crop    123.3 Adult Midgut    75.1 Adult Hindgut    135.8 Adult Malpighian Tubules    149.5 Adult Fat Body    148.8 Adult Salivary Gland    225.4 Adult Heart    218.175 Adult VirginFemale Spermatheca    67.1 Adult InseminatedFemale Spermatheca    89 Adult Ovary    111.9 Adult Testis    67.4 Adult Male Accessory Gland    109 Adult Carcass    109.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    89.05 Larval Midgut    102.4 Larval Hindgut    128.2 Larval Malpighian Tubules    208.7 Larval Fat Body    86 Larval Salivary Gland    162.7 Larval Trachea    111.05 Larval Carcass    122.025 Adult Head    77.1 Adult Eye    88.775 Adult Brain    69.9 Adult Thoracic-Abdominal Ganglion    58.1 Adult Crop    123.3 Adult Midgut    75.1 Adult Hindgut    135.8 Adult Malpighian Tubules    149.5 Adult Fat Body    148.8 Adult Salivary Gland    225.4 Adult Heart    218.175 Adult VirginFemale Spermatheca    67.1 Adult InseminatedFemale Spermatheca    89 Adult Ovary    111.9 Adult Testis    67.4 Adult Male Accessory Gland    109 Adult Carcass    109.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    89.05 Larval Midgut    102.4 Larval Hindgut    128.2 Larval Malpighian Tubules    208.7 Larval Fat Body    86 Larval Salivary Gland    162.7 Larval Trachea    111.05 Larval Carcass    122.025 Adult Head    77.1 Adult Eye    88.775 Adult Brain    69.9 Adult Thoracic-Abdominal Ganglion    58.1 Adult Crop    123.3 Adult Midgut    75.1 Adult Hindgut    135.8 Adult Malpighian Tubules    149.5 Adult Fat Body    148.8 Adult Salivary Gland    225.4 Adult Heart    218.175 Adult VirginFemale Spermatheca    67.1 Adult InseminatedFemale Spermatheca    89 Adult Ovary    111.9 Adult Testis    67.4 Adult Male Accessory Gland    109 Adult Carcass    109.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    77.1   NA  Eye    88.775   NA  Brain    69.9   89.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    58.1   NA  Crop    123.3   102.4  Midgut    75.1   128.2  Hindgut    135.8   208.7  Malpighian Tubules    149.5   86  Fat Body    148.8   162.7  Salivary Gland    225.4   NA  Heart    218.175   111.05  Trachea    NA   NA  VirginFemale Spermatheca    67.1   NA  InseminatedFemale Spermatheca    89   NA  Ovary    111.9   NA  Testis    67.4   NA  Male Accessory Gland    109   122.025  Carcass    109.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    19 central nervous system, pupae P8    11 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    14 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    12 head, mated 20-day male    17 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    17 digestive system, 4-day adult    14 digestive system, 20-day adult    16 fat body, larvae L3 wandering    9 fat body, white prepupae    16 fat body, pupae P8    7 carcass, larvae L3 wandering    21 carcass, 1-day adult    10 carcass, 4-day adult    12 carcass, 20-day adult    16 ovary, virgin 4-day female    30 ovary, mated 4-day female    20 testis, mated 4-day male    28 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    19 central nervous system, pupae P8    11 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    14 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    12 head, mated 20-day male    17 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    17 digestive system, 4-day adult    14 digestive system, 20-day adult    16 fat body, larvae L3 wandering    9 fat body, white prepupae    16 fat body, pupae P8    7 carcass, larvae L3 wandering    21 carcass, 1-day adult    10 carcass, 4-day adult    12 carcass, 20-day adult    16 ovary, virgin 4-day female  (30) ovary, mated 4-day female    20 testis, mated 4-day male  (28) accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    19 central nervous system, pupae P8    11 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    14 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    12 head, mated 20-day male    17 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    17 digestive system, 4-day adult    14 digestive system, 20-day adult    16 fat body, larvae L3 wandering    9 fat body, white prepupae    16 fat body, pupae P8    7 carcass, larvae L3 wandering    21 carcass, 1-day adult    10 carcass, 4-day adult    12 carcass, 20-day adult    16 ovary, virgin 4-day female    30 ovary, mated 4-day female    20 testis, mated 4-day male    28 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    19 central nervous system, pupae P8    11 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    14 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    12 head, mated 20-day male    17 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    17 digestive system, 4-day adult    14 digestive system, 20-day adult    16 fat body, larvae L3 wandering    9 fat body, white prepupae    16 fat body, pupae P8    7 carcass, larvae L3 wandering    21 carcass, 1-day adult    10 carcass, 4-day adult    12 carcass, 20-day adult    16 ovary, virgin 4-day female    30 ovary, mated 4-day female    20 testis, mated 4-day male    28 accessory gland, mated 4-day male    16 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    19 central nervous system, pupae P8    11 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    14 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    12 head, mated 20-day male    17 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    17 digestive system, 4-day adult    14 digestive system, 20-day adult    16 fat body, larvae L3 wandering    9 fat body, white prepupae    16 fat body, pupae P8    7 carcass, larvae L3 wandering    21 carcass, 1-day adult    10 carcass, 4-day adult    12 carcass, 20-day adult    16 ovary, virgin 4-day female    30 ovary, mated 4-day female    20 testis, mated 4-day male    28 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    19 central nervous system, pupae P8    11 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    14 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    12 head, mated 20-day male    17 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    17 digestive system, 4-day adult    14 digestive system, 20-day adult    16 fat body, larvae L3 wandering    9 fat body, white prepupae    16 fat body, pupae P8    7 carcass, larvae L3 wandering    21 carcass, 1-day adult    10 carcass, 4-day adult    12 carcass, 20-day adult    16 ovary, virgin 4-day female  30 ovary, mated 4-day female    20 testis, mated 4-day male  28 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    19 central nervous system, pupae P8    11 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    14 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    12 head, mated 20-day male    17 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    17 digestive system, 4-day adult    14 digestive system, 20-day adult    16 fat body, larvae L3 wandering    9 fat body, white prepupae    16 fat body, pupae P8    7 carcass, larvae L3 wandering    21 carcass, 1-day adult    10 carcass, 4-day adult    12 carcass, 20-day adult    16 ovary, virgin 4-day female    30 ovary, mated 4-day female    20 testis, mated 4-day male    28 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    19 central nervous system, pupae P8    11 head, virgin 1-day female    12 head, virgin 4-day female    9 head, virgin 20-day female    14 head, mated 1-day female    8 head, mated 4-day female    8 head, mated 20-day female    11 head, mated 1-day male    14 head, mated 4-day male    12 head, mated 20-day male    17 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    17 digestive system, 4-day adult    14 digestive system, 20-day adult    16 fat body, larvae L3 wandering    9 fat body, white prepupae    16 fat body, pupae P8    7 carcass, larvae L3 wandering    21 carcass, 1-day adult    10 carcass, 4-day adult    12 carcass, 20-day adult    16 ovary, virgin 4-day female    30 ovary, mated 4-day female    20 testis, mated 4-day male    28 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    14 embryo 04-06hr    31 embryo 06-08hr    35 embryo 08-10hr    36 embryo 10-12hr    49 embryo 12-14hr    34 embryo 14-16hr    40 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    18 embryo 22-24hr    19 larva L1    22 larva L2    31 larva L3 12hr old    22 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    15 pupae 4d postWPP    13 adult male 01day    20 adult male 05day    21 adult male 30day    21 adult female 01day    22 adult female 05day    28 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    14 embryo 04-06hr  (31) embryo 06-08hr  (35) embryo 08-10hr  (36) embryo 10-12hr  (49) embryo 12-14hr  (34) embryo 14-16hr  (40) embryo 16-18hr  (29) embryo 18-20hr    24 embryo 20-22hr    18 embryo 22-24hr    19 larva L1    22 larva L2  (31) larva L3 12hr old    22 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    15 pupae 4d postWPP    13 adult male 01day    20 adult male 05day    21 adult male 30day    21 adult female 01day    22 adult female 05day  (28) adult female 30day  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    14 embryo 04-06hr    31 embryo 06-08hr    35 embryo 08-10hr    36 embryo 10-12hr    49 embryo 12-14hr    34 embryo 14-16hr    40 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    18 embryo 22-24hr    19 larva L1    22 larva L2    31 larva L3 12hr old    22 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    15 pupae 4d postWPP    13 adult male 01day    20 adult male 05day    21 adult male 30day    21 adult female 01day    22 adult female 05day    28 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    14 embryo 04-06hr    31 embryo 06-08hr    35 embryo 08-10hr    36 embryo 10-12hr    49 embryo 12-14hr    34 embryo 14-16hr    40 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    18 embryo 22-24hr    19 larva L1    22 larva L2    31 larva L3 12hr old    22 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    15 pupae 4d postWPP    13 adult male 01day    20 adult male 05day    21 adult male 30day    21 adult female 01day    22 adult female 05day    28 adult female 30day    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    14 embryo 04-06hr    31 embryo 06-08hr    35 embryo 08-10hr    36 embryo 10-12hr    49 embryo 12-14hr    34 embryo 14-16hr    40 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    18 embryo 22-24hr    19 larva L1    22 larva L2    31 larva L3 12hr old    22 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    15 pupae 4d postWPP    13 adult male 01day    20 adult male 05day    21 adult male 30day    21 adult female 01day    22 adult female 05day    28 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    14 embryo 04-06hr  31 embryo 06-08hr  (35) embryo 08-10hr  (36) embryo 10-12hr  (49) embryo 12-14hr  (34) embryo 14-16hr  (40) embryo 16-18hr  29 embryo 18-20hr    24 embryo 20-22hr    18 embryo 22-24hr    19 larva L1    22 larva L2  31 larva L3 12hr old    22 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    15 pupae 4d postWPP    13 adult male 01day    20 adult male 05day    21 adult male 30day    21 adult female 01day    22 adult female 05day  28 adult female 30day  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    14 embryo 04-06hr    31 embryo 06-08hr    35 embryo 08-10hr    36 embryo 10-12hr    49 embryo 12-14hr    34 embryo 14-16hr    40 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    18 embryo 22-24hr    19 larva L1    22 larva L2    31 larva L3 12hr old    22 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    15 pupae 4d postWPP    13 adult male 01day    20 adult male 05day    21 adult male 30day    21 adult female 01day    22 adult female 05day    28 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    14 embryo 04-06hr    31 embryo 06-08hr    35 embryo 08-10hr    36 embryo 10-12hr    49 embryo 12-14hr    34 embryo 14-16hr    40 embryo 16-18hr    29 embryo 18-20hr    24 embryo 20-22hr    18 embryo 22-24hr    19 larva L1    22 larva L2    31 larva L3 12hr old    22 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    19 white prepupae 24hr    22 pupae 2d postWPP    23 pupae 3d postWPP    15 pupae 4d postWPP    13 adult male 01day    20 adult male 05day    21 adult male 30day    21 adult female 01day    22 adult female 05day    28 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    50 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    54 embryonic S1    54 embryonic S3    31 leg disc CME L1    42 wing disc CME-W2    49 wing disc ML-DmD8    31 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    34 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    34 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    42 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    14 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4  (50) embryonic 1182-4H  (41) embryonic GM2  (78) embryonic Kc167  (54) embryonic S1  (54) embryonic S3  (31) leg disc CME L1  (42) wing disc CME-W2  (49) wing disc ML-DmD8  (31) wing disc ML-DmD9    0 wing disc ML-DmD16-c3  (27) wing disc ML-DmD21  (34) wing disc ML-DmD32  (48) haltere disc ML-DmD17-c3  (34) eye-antennal disc ML-DmD11  (46) antennal disc ML-DmD20-c5  (47) mixed discs ML-DmD4-c1  (41) CNS ML-DmBG1-c1  (42) CNS ML-DmBG2-c2  (30) tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    14 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    50 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    54 embryonic S1    54 embryonic S3    31 leg disc CME L1    42 wing disc CME-W2    49 wing disc ML-DmD8    31 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    34 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    34 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    42 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    14 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    50 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    54 embryonic S1    54 embryonic S3    31 leg disc CME L1    42 wing disc CME-W2    49 wing disc ML-DmD8    31 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    34 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    34 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    42 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    14 ovary OSS    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    50 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    54 embryonic S1    54 embryonic S3    31 leg disc CME L1    42 wing disc CME-W2    49 wing disc ML-DmD8    31 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    34 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    34 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    42 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    14 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4  (50) embryonic 1182-4H  (41) embryonic GM2  (78) embryonic Kc167  (54) embryonic S1  (54) embryonic S3  31 leg disc CME L1  (42) wing disc CME-W2  (49) wing disc ML-DmD8  31 wing disc ML-DmD9    0 wing disc ML-DmD16-c3  27 wing disc ML-DmD21  (34) wing disc ML-DmD32  (48) haltere disc ML-DmD17-c3  (34) eye-antennal disc ML-DmD11  (46) antennal disc ML-DmD20-c5  (47) mixed discs ML-DmD4-c1  (41) CNS ML-DmBG1-c1  (42) CNS ML-DmBG2-c2  30 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    14 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    50 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    54 embryonic S1    54 embryonic S3    31 leg disc CME L1    42 wing disc CME-W2    49 wing disc ML-DmD8    31 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    34 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    34 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    42 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    14 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    24 Schneider line 2 Sg4    50 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    54 embryonic S1    54 embryonic S3    31 leg disc CME L1    42 wing disc CME-W2    49 wing disc ML-DmD8    31 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    34 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    34 eye-antennal disc ML-DmD11    46 antennal disc ML-DmD20-c5    47 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    42 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    14 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult  (31) Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult  31 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    14 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    24 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_03 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013998 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG33101-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | germline clone | larval stage | recessive Nsf255 lethal | larval stage | recessive Nsf211 Nsf215 Nsf221 Nsf255 Nsf2102 Nsf2103 lethal | pharate adult stage | recessive Nsf242 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Nsf2GD1327 viable Nsf2A6 Other Phenotypes Allele neuroanatomy defective, with Scer\GAL4elav.PLu Nsf2EQ.Scer\UAS neuroanatomy defective | larval stage, with Scer\GAL4elav.PLu Nsf2EQ.Scer\UAS neurophysiology defective | larval stage, with Scer\GAL4elav.PLu Nsf2EQ.Scer\UAS neurophysiology defective | larval stage, with Scer\GAL4elav-C155 Nsf2EQ.Scer\UAS visible Nsf2A6 visible, with Scer\GAL4bbg-C96 Nsf2EQ.Scer\UAS visible, with Scer\GAL4pnr-MD237 Nsf2GD1327 wild-type Nsf2hs.PG wild-type, with Scer\GAL4bbg-C96 Nsf2WT.Scer\UAS Phenotype manifest in Allele embryonic/larval neuromuscular junction, with Scer\GAL4BG487 Nsf2EQ.Scer\UAS embryonic/larval neuromuscular junction, with Scer\GAL4elav.PLu Nsf2EQ.Scer\UAS eye Nsf2A6 mesothoracic tergum, with Scer\GAL4pnr-MD237 Nsf2GD1327 synaptic vesicle, with Scer\GAL4elav-C155 Nsf2EQ.Scer\UAS trichogen cell, with Scer\GAL4pnr-MD237 Nsf2GD1327 wing margin, with Scer\GAL4bbg-C96 Nsf2EQ.Scer\UAS
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of Nsf2 Class Mutagen Stocks Known lesion Nsf2A15 ethyl methanesulfonate 1 Yes Nsf2A6 ethyl methanesulfonate 1 Yes Nsf2102 ethyl methanesulfonate 0 Yes Nsf2103 loss of function allele ethyl methanesulfonate 0 Yes Nsf211 ethyl methanesulfonate 0 Yes Nsf215 loss of function allele ethyl methanesulfonate 0 Yes Nsf221 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Nsf242 ethyl methanesulfonate 0 Yes Nsf255 loss of function allele ethyl methanesulfonate 0 Yes Nsf266 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nsf2 Class Mutagen Stocks Known lesion Nsf2GD1327 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nsf2HMS01262 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nsf2JF02765 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nsf2EQ.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Nsf2hs.PG in vitro construct - regulatory fusion 0 Yes Nsf2Scer\UAS.cGa in vitro construct - regulatory fusion 0 Yes Nsf2WT.Scer\UAS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)BSC611 (Cook et al., 2008.9.3) Df(3R)urd (Golby et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1327} NA P{TRiP.HMS01262} NA P{TRiP.JF02765} NA P{UAS-Nsf2.EQ} NA P{UAS-Nsf2.G} NA P{UAS-Nsf2.WT} NA heat-shock construct P{hsp70-Nsf2.G} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
neuromuscular synaptic transmission inferred from mutant phenotype (Stewart et al., 2002) regulation of synaptic growth at neuromuscular junction inferred from mutant phenotype (Stewart et al., 2002)
Cellular Component
CV term Evidence References
colocalizes_with SNARE complex inferred from direct assay (Guruharsha et al., 2011)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960 (FlyBase Curators et al., 2004-) nucleoside-triphosphatase activity inferred from electronic annotation with InterPro:IPR003593 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
ER to Golgi vesicle-mediated transport inferred from sequence or structural similarity with UniProtKB:P18708 (FlyBase Genome Annotators, 2002-2003) non-traceable author statement (Swiss-Prot Project Members, 2001.8.20) intra-Golgi vesicle-mediated transport non-traceable author statement (Swiss-Prot Project Members, 2001.8.20) neurotransmitter secretion non-traceable author statement (Lloyd et al., 2000) synaptic vesicle priming non-traceable author statement (Lloyd et al., 2000) vesicle-mediated transport non-traceable author statement (Swiss-Prot Project Members, 2001.8.20)
Cellular Component
CV term Evidence References
cytoplasm inferred from sequence or structural similarity with UniProtKB:P18708 (FlyBase Genome Annotators, 2002-2003) non-traceable author statement (Littleton et al., 1999, Swiss-Prot Project Members, 2001.8.20)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Nsf2 - Bet1 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - CG1599 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - CG2023 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - CG6208 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - Sec22 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - Slh experimental knowledge based (Guruharsha et al., 2011) Nsf2 - Snap experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Nsf2 - Snap25 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - Syx18 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - Syx1A anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Nsf2 - Syx5 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - Syx8 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - Use1 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - Ykt6 experimental knowledge based (Guruharsha et al., 2011) Nsf2 - membrin experimental knowledge based (Guruharsha et al., 2011) Nsf2 - usnp experimental knowledge based (Guruharsha et al., 2011) Nsf2 - γSnap anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nsf2 allele Gene References Nsf215 comt (Golby et al., 2001) Nsf255 comt (Golby et al., 2001) Nsf2103 comt (Golby et al., 2001) Nsf2EQ.Scer\UAS Syx5 (Xu et al., 2002) Syx16 (Xu et al., 2002) wg (Stewart et al., 2001) Nsf2Scer\UAS.cGa comt (Golby et al., 2001) unspecified Act5C (Laviolette et al., 2005) Bacc (Laviolette et al., 2005) Baldspot (Laviolette et al., 2005) ben (Laviolette et al., 2005) brat (Laviolette et al., 2005) btn (Laviolette et al., 2005) C3G (Laviolette et al., 2005) CdsA (Laviolette et al., 2005) CG1806 (Laviolette et al., 2005) CG1888 (Laviolette et al., 2005) CG3603 (Laviolette et al., 2005) CG4119 (Laviolette et al., 2005) CG6388 (Laviolette et al., 2005) CG7492 (Laviolette et al., 2005) CG10585 (Laviolette et al., 2005) CG10801 (Laviolette et al., 2005) CG11897 (Laviolette et al., 2005) CG12264 (Laviolette et al., 2005) CG14411 (Laviolette et al., 2005) CG14628 (Laviolette et al., 2005) CG14651 (Laviolette et al., 2005) CG32650 (Laviolette et al., 2005) CG42450 (Laviolette et al., 2005) CG43462 (Laviolette et al., 2005) cpo (Laviolette et al., 2005) Cyp6v1 (Laviolette et al., 2005) E2f (Laviolette et al., 2005) fend (Laviolette et al., 2005) Fim (Laviolette et al., 2005) fz4 (Laviolette et al., 2005) Gdi (Laviolette et al., 2005) Gli (Laviolette et al., 2005) grp (Laviolette et al., 2005) GS32 (Laviolette et al., 2005) GS1027 (Laviolette et al., 2005) GS2192 (Laviolette et al., 2005) GS2285 (Laviolette et al., 2005) GS3087 (Laviolette et al., 2005) GS5036 (Laviolette et al., 2005) GS5071 (Laviolette et al., 2005) GS8172 (Laviolette et al., 2005) GS14505 (Laviolette et al., 2005) igl (Laviolette et al., 2005) kuz (Laviolette et al., 2005) l(2)37Cg (Laviolette et al., 2005) LanA (Laviolette et al., 2005) lola (Laviolette et al., 2005) Lon (Laviolette et al., 2005) Moe (Laviolette et al., 2005) Mppe (Laviolette et al., 2005) nej (Laviolette et al., 2005) NetB (Laviolette et al., 2005) ovo (Laviolette et al., 2005) phtf (Laviolette et al., 2005) polo (Laviolette et al., 2005) Psn (Mahoney et al., 2006) Rab5 (Laviolette et al., 2005) Rbf (Laviolette et al., 2005) RhoBTB (Laviolette et al., 2005) RpS2 (Laviolette et al., 2005) Snap (Laviolette et al., 2005, Babcock et al., 2004) spi (Laviolette et al., 2005) Syn2 (Laviolette et al., 2005) Tcp-1η (Laviolette et al., 2005) Ubi-p5E (Laviolette et al., 2005) ubl (Laviolette et al., 2005) Ufd1-like (Laviolette et al., 2005) wuho (Laviolette et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66772 DroID - A comprehensive database of gene and protein interactions. • FBgn0013998 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41694
Orthologs
OrthoDB Orthologs (46) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6HMJVQ Dipteran inclusive ortholog search EOG68PMT8 Insect inclusive ortholog search EOG6XGXV2 Arthropod inclusive ortholog search EOG6K6DK6 Metazoa inclusive ortholog search EOG696BJZ
Orthologs in Drosophila Species (EOG6HMJVQ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Nsf2     Drosophila simulans   Dsim\GD20473 Y   Drosophila sechellia   Dsec\GM25905 Y   Drosophila erecta   Dere\GG17019     Drosophila yakuba   Dyak\GE24413 Y   Drosophila ananassae   Dana\GF16398     Drosophila pseudoobscura pseudoobscura   Dpse\GA17281     Drosophila persimilis   Dper\GL24082     Drosophila willistoni   Dwil\GK13058     Drosophila virilis   Dvir\GJ14275     Drosophila mojavensis   Dmoj\GI24416     Drosophila grimshawi   Dgri\GH14042
Orthologs in non-Drosophila Dipterans (EOG68PMT8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001616 Y Anopheles gambiae Malaria mosquito  Agam\AGAP012684 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003150 Y
Orthologs in non-Dipteran Insects (EOG6XGXV2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC725680 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG014976 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12468 Y Atta cephalotes Leafcutter ant  Acep\ACEP14455 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO16622 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL12891 Y Linepithema humile Argentine ant  Lhum\LH20300 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB23268 Y Solenopsis invicta Red fire ant  Sinv\SINV16762 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI009908 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000447 Y Pediculus humanus Human body louse  Phum\PHUM047300 Y Pediculus humanus Human body louse  Phum\PHUM047310 Y Tribolium castaneum Red flour beetle  Tcas\TC012279 Y
Orthologs in non-Insect Arthropods (EOG6K6DK6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_118650 Y Daphnia pulex Water flea  Dpux\JGI_V11_240595 Y Daphnia pulex Water flea  Dpux\cytb Y Daphnia pulex Water flea  Dpux\JGI_V11_240520 Y Daphnia pulex Water flea  Dpux\JGI_V11_240599 Y Ixodes scapularis Deer tick  Isca\ISCW024697 Y Ixodes scapularis Deer tick  Isca\ISCW007723 Y
Orthologs in non-Arthropod Metazoa (EOG696BJZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\nsf-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021816gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007303gn Y Danio rerio Zebrafish  Drer\nsfa Y Danio rerio Zebrafish  Drer\nsfb Y Xenopus tropicalis Western clawed frog  Xtro\nsf Y Gallus gallus Domestic chicken  Ggal\NSF Y Mus musculus House mouse  Mmus\Nsf Y Rattus norvegicus Norway rat  Rnor\Nsf Y Homo sapiens Human  Hsap\NSF Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\NSF
AAA Orthologs (3) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20473 Drosophila sechellia Dsec\GM25905 Drosophila yakuba Dyak\GE24413
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	27685 y1 v1; P{TRiP.JF02765}attP2 8282 w1118; Nsf2A15/TM6B, Tb1 34914 y1 sc* v1; P{TRiP.HMS01262}attP2 8281 w1118; Nsf2A6/TM3, Sb1 Ser1
VDRC	v7743 w1118; P{GD1327}v7743
Genomic Clones ( 2 )
	BACR25B05 BACR30G22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 151 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD09622 RE33604
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT13474 AT28554 GH09211 GM15043 GM26786 GM32081 HL04960 HL05916 LD11372 LD15205 LD19337 LD31558 RE03575 RE06747 RE17633 RE17645 RE18385 RE18950 RE27527 RE61865 RH27115 RH33585 RH59117
Other clones	15159 18371 39991 62373 104310 523390 bs40g03 EK040110 EK129016 EK154262 EK188053 EK229162 EN03834 FGC022E07 RP003005599 RP003006587 RP003012075 RP003012947 RP003026421 RP003041270 RP003043671 RP003043712 RP003046017 RP003059766 RP003063267 RP003068482 RP003072537 RP003080004 RP003080201 RP003086723 RP003096208 RP003099057 RP003100868 RP003101833 RP003106698 RP003110877 RP003111278 RP003113454 RP003117192 RP003120233 RP003134012 RP003143349 RP003143360 RP003143929 RP003150946 RP003172331 RP003178905 RP003190842 RP003193643 RP003196437 RP003211708 RP003215099 RP003217303 RP003228373 RP003229135 RP003230638 RP003245670 RP003246156 RP003246788 RP003251482 RP003253247 RP003253607 RP003260108 RP003261192 RP003268661 RP003274561 RP003275669 RP003290511 RP003294931 RP003295629 RP003301735 RP003306501 RP003313205 RP003315413 RP003315658 RP003320288 RP003327629 RP003327671 RP003328142 RP003330660 RP003366554 RP003374478 RP003383114 RP003432293 RP003435626 RP003437521 RP003441417 RP003471171 RP003475869 RP003480065 RP003483049 RP003483763 RP003487397 RP003496560 RP003498882 RP003499761 RP003501700 RP003508078 RP003511515 RP003511569 RP003514555 RP003524245 RP003525586 RP003526353 RP003526974 RP003529098 RP003531363 RP003533530 RP003545798 RP003553512 RP003557884 RP003561163 RP003564220 RP003573337 RP003575400 RP003578904 RP003586479 RP003602023 RP003605309 RP003617322 RP003637264 RP003640616 RP003640680 RP003664733 RP003675918 RP003682418
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0013998 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41694
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Nsf2 CG9931 (Zheng, 1999.11)
Source for database merge of
Additional comments	Annotation CG9931 split into CG33101 (Nsf2) and CG31495 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	ChEST reveals this is a target of Mef2. (Junion et al., 2005) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per01, tim01 and increased expression in ClkJrk background. (Claridge-Chang et al., 2001) comt and Nsf2 exhibit similar functional properties but have evolved distinct tissue-specific roles. comt is required in the nervous system beginning at the adult stage of development whereas Nsf2 is required in the mesoderm beginning at the first larval instar. The comt and Nsf2 proteins can functionally substitute for one another. (Golby et al., 2001) Evidence of multiple, highly related isoforms of NSF in a single species suggests that in some tissue membranes fusion and/or secretion has a specific role during development. (Boulianne and Trimble, 1995) Drosophila has a second N-ethylmaleimide-sensitive fusion protein (NSF) homolog, Nsf2. comt and Nsf2 display overlapping but different temporal expression patterns. Multiple transcripts derived from Nsf2. (Pallanck et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_176499 RefSeq (Proteins) • NP_788676 DDBJ / EMBL / GenBank • AAA75044 AAC46844 AAF54995 U28836 U30502 Entrez Gene - A searchable database of RefSeq genes. • 41694 UniProtKB/Swiss-Prot • P54351
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • CDC48, N-terminal subdomain (IPR003338) AAA+ ATPase domain (IPR003593) ATPase, AAA-type, core (IPR003959) ATPase, AAA-type, conserved site (IPR003960) CDC48, domain 2 (IPR004201) Aspartate decarboxylase-like domain (IPR009010) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Linkouts
	BioGRID - A database of protein and genetic interactions • 66772 DroID - A comprehensive database of gene and protein interactions. • FBgn0013998 DRSC - Results from RNAi screens. • FBgn0013998 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013998 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG33101-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013998 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41694 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/nsf1-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41694 modMine - Data generated by the modENCODE project. • FBgn0013998
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	CG9931 (Dunne et al., 2002, Verstreken and Bellen, 2000.9.4, Dorer et al., 2006) CG33101 (Perkins et al., 2009, Ni et al., 2011.7.19, Cammarato et al., 2011) DmNSF2 (Frohlich, 2001) dNSF (Wu and Bellen, 1997) dNSF2 (Xu et al., 2002, Stewart, 2002, Golby et al., 2001, Dunne and Rabouille, 2001, Stewart et al., 2001, Golby et al., 2000, Tolar et al., 1999, Babcock et al., 2004, Stewart et al., 2002, Stewart et al., 2005) dnsf2 (Dunne et al., 2002) dNSF-2 (Mohtashami et al., 2000, Trimble, 1995.6.8, Pallanck, 1995.6.28, Mohtashami and Trimble, 1998, Pallanck et al., 1995, Boulianne and Trimble, 1995, Boulianne and Trimble, 1995, ) dNsf2 (Golby et al., 2001) Nsf-2 (Junion et al., 2005) NSF2 (Nunes and Stewart, 2005, Laviollette et al., 2005, Stewart et al., 2003, Bellen, 2000.12.25, Patel and Latterich, 1998, Swaffield and Purugganan, 1997, Laviolette et al., 2005, Seabrooke and Stewart, 2008, Peyre et al., 2006, Nunes et al., 2006, Dorer et al., 2006, Stewart et al., 2005, Nagai et al., 2010) NSF-2 (Lloyd et al., 2000) Nsf2 (Mahoney et al., 2006, Cook et al., 2008.9.3, Stuart et al., 2007, Galindo et al., 2007, Liebl and Featherstone, 2008, Guruharsha et al., 2011, Kennison, 2012.9.27)
Name Synonym	NEM-sensitive fusion protein 2 (Boulianne and Trimble, 1995, Cammarato et al., 2011)
Secondary FlyBase IDs
	FBgn0053101
References ( 68 )
Generate a list of	All references relating to this gene ( 68 )
List References by type	Research paper  ( 26 ) Supplementary material  ( 7 ) Review  ( 5 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 11 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Letter  ( 1 )
Recent research papers ( 1 )
	Guruharsha et al., 2011, Cell 147(3): 690--703 A Protein Complex Network of Drosophila melanogaster. [FBrf0216491] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...