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General Information
Symbol
Dmel\Rho1
Species
D. melanogaster
Name
Rho1
Annotation Symbol
CG8416
Feature Type
FlyBase ID
FBgn0014020
Gene Model Status
Stock Availability
Gene Snapshot
Rho1 (Rho1) encodes a GTPase signaling protein that acts as a molecular switch and functions as key regulator of the actin cytoskeleton. It plays a central role in diverse biological processes including actin cytoskeleton organization, microtubule dynamics, mophogenesis, border cell migration, cytokinesis, and wound repair. [Date last reviewed: 2019-03-14]
Also Known As

RhoA, Rho, Rho GTPase, DrhoA, Rho kinase

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:16,102,687..16,107,229 [+]
Recombination map

2-77

RefSeq locus
NT_033778 REGION:16102687..16107229
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (89 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from genetic interaction with FLYBASE:cv-c; FB:FBgn0285955
inferred from genetic interaction with FLYBASE:RhoGEF64C; FB:FBgn0035574
inferred from direct assay
inferred from genetic interaction with FLYBASE:RhoGAPp190; FB:FBgn0026375
inferred from physical interaction with FLYBASE:Rok; FB:FBgn0026181
inferred from physical interaction with FLYBASE:wash; FB:FBgn0033692
inferred from physical interaction with UniProtKB:A1Z7T0
(assigned by UniProt )
inferred from physical interaction with UniProtKB:P48554
(assigned by UniProt )
inferred from physical interaction with FLYBASE:Rok; FB:FBgn0026181
inferred from physical interaction with FLYBASE:pbl; FB:FBgn0003041
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000632671
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000632671
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
Biological Process (72 terms)
Terms Based on Experimental Evidence (65 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:fz; FB:FBgn0001085
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Rok; FB:FBgn0026181
inferred from genetic interaction with FLYBASE:kirre; FB:FBgn0028369
inferred from genetic interaction with FLYBASE:Ced-12; FB:FBgn0032409
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from high throughput mutant phenotype
Terms Based on Predictions or Assertions (9 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001178055
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR003578
(assigned by InterPro )
Cellular Component (12 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from direct assay
colocalizes_with apical plasma membrane
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (8 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001530052
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the small GTPase superfamily. Rho family. (P48148)
Summaries
Gene Group (FlyBase)
RHO GTPASES -
Rho (Ras Homologous) GTPases are members of the Ras superfamily of small GTPases. They are best characterized for their roles in regulating actin organization. (Adapted from PMID:15731001).
Pathway (FlyBase)
Negative Regulators of Wnt-TCF Signaling Pathway -
Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Protein Function (UniProtKB)
Has a role in regulating actin cytoskeletal organization: required during early development for proper execution of morphogenetic movements of individual cells and groups of cells important for the formation of the embryonic body plan (PubMed:10556060, PubMed:24535826, PubMed:25739458). Plays a role in regulating dorsal closure during embryogenesis (PubMed:10556060, PubMed:10323867). During axis elongation, required for Rho-kinase Rok planar polarity and adherens junction localization as well as for generating a planar polarized distribution of the actin-binding protein Shrm (PubMed:24535826). During embryogenesis, acts upstream of wash to regulate the developmental migration of tail hemocytes anteriorly along the ventral midline (PubMed:25739458). May have a role in eye development (PubMed:7835340).
(UniProt, P48148)
Summary (Interactive Fly)

rho-subfamily GTPase - a molecular switch and functions as key regulator of the actin cytoskeleton in concert with DRhoGEF2, Rho1 mediates specific cell shape changes during gastrulation in response to the extracellular ligand, Folded gastrulation - plays a central role in actin cytoskeleton organization, microtubule dynamics, mophogenesis, border cell migration, cytokinesis, and wound repair

Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Rho1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0087211
1288
192
FBtr0087212
2028
192
FBtr0087213
1899
192
FBtr0087214
1170
192
FBtr0087216
1971
192
FBtr0087217
1977
192
Additional Transcript Data and Comments
Reported size (kB)

3.7, 3.1, 2.1 (northern blot); 1.3 (unknown)

1.467 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0086353
21.7
192
7.06
FBpp0086354
21.7
192
7.06
FBpp0086355
21.7
192
7.06
FBpp0086356
21.7
192
7.06
FBpp0089129
21.7
192
7.06
FBpp0089130
21.7
192
7.06
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with capu (PubMed:10556060). Interacts (via REM repeats) with Pkn (via N-terminus) (PubMed:10323867, PubMed:17507675). Interacts (via N-terminus) with wash (via N-terminus) (PubMed:25739458).

(UniProt, P48148)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rho1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Rho1 protein shows apical localization in tracheal tubes.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
colocalizes_with apical plasma membrane
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rho1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 37 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 41 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rho1
Transgenic constructs containing regulatory region of Rho1
Deletions and Duplications ( 11 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
actin filament & embryonic/larval tracheal system, with Scer\GAL4btl.PS
cortical actin cytoskeleton & embryonic posterior spiracle, with Scer\GAL4ems.HRE
cortical actin cytoskeleton & spiracular chamber (with Rho11B)
cortical actin cytoskeleton & spiracular chamber (with Rho172R)
disc epithelium proper & dorsal mesothoracic disc, with Scer\GAL4ptc-559.1
embryonic/first instar larval cuticle & embryonic head
eye disc & photoreceptor cell & axon, with Scer\GAL4GMR.PF
eye disc & subretinal glial cell, with Scer\GAL4GMR.PF
mushroom body & dendrite, with Scer\GAL4Tab2-201Y
mushroom body & dendrite | somatic clone
mushroom body & dendrite | somatic clone, with Scer\GAL4Tab2-201Y
mushroom body & neuron | somatic clone
sensory neuron & axon, with Scer\GAL4repo
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (22)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
4  
12 of 15
No
Yes
1  
9 of 15
No
Yes
1  
3 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
0  
1 of 15
No
No
3  
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (22)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
12 of 15
No
Yes
10 of 15
No
Yes
8 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (19)
10 of 13
Yes
Yes
10 of 13
Yes
Yes
10 of 13
Yes
Yes
7 of 13
No
Yes
3 of 13
No
Yes
2 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (17)
13 of 12
Yes
Yes
10 of 12
No
Yes
10 of 12
No
Yes
9 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (39)
14 of 15
Yes
Yes
13 of 15
No
Yes
12 of 15
No
Yes
12 of 15
No
Yes
12 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (7)
14 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (6)
12 of 15
Yes
Yes
5 of 15
No
Yes
4 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
1 of 15
No
No
1  
Schizosaccharomyces pombe (Fission yeast) (5)
10 of 12
Yes
Yes
9 of 12
No
Yes
3 of 12
No
Yes
3 of 12
No
Yes
1 of 12
No
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190FFR )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150B5M )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CVO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0CTS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0QVS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (8)
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
2 of 10
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 8 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    RNA-protein
    Physical Interaction
    Assay
    References
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Interacts with capu (PubMed:10556060). Interacts (via REM repeats) with Pkn (via N-terminus) (PubMed:10323867, PubMed:17507675). Interacts (via N-terminus) with wash (via N-terminus) (PubMed:25739458).
    (UniProt, P48148 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map

    2-77

    Cytogenetic map
    Sequence location
    2R:16,102,687..16,107,229 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    52E5-52E5
    Limits computationally determined from genome sequence between P{PZ}l(2)0507005070&P{EP}CG8414EP525 and P{EP}spinEP645&P{lacW}spink09905
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Cytological location reported in FBrf0080078 was in error.
    52E-52E
    (determined by in situ hybridisation)
    52E5-52E6
    (determined by in situ hybridisation)
    52E3-52E6
    (determined by in situ hybridisation)
    52E2-52E9
    (determined by in situ hybridisation)
    52D-52D
    (determined by in situ hybridisation)
    3C-3C
    (determined by in situ hybridisation)
    Hybridization of Rho1 cDNA to Df(2R)KL32 and Df(2R)Jp6 confirmed that Rho1 maps to 52E.
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (47)
    Genomic Clones (21)
    cDNA Clones (146)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of

    Source for merge of: Rho1 l(2)k02107b

    Source for merge of: Rho1 l(2)52Fa

    Additional comments
    Other Comments

    sti is necessary for correct Rho1 localisation at the cleavage site in late telophase.

    Rho1 is an upstream regulator of DAAM.

    The active Rho1 product promotes microtubule/microfilament crosslinking by sequestering the spir-PD isoform, preventing this protein from binding to spir-PC and capu.

    Rho1 regulates shg but not arm localisation and the effect of loss of Rho1 is not enhanced or suppressed by removing p120ctn.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes no cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

    dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: binucleate cells with increased or polarized (uneven) accumulation of F-actin.

    Rho1 and Rac1 mediate the dynamic rearrangement of actin in peripheral glia.

    Rho1 appears to be required for the regulation of actin polymerisation and/or myosin activity that is critical for thr response of growth cones to midline repulsive signals.

    Rho1 is necessary for normal morphology and behaviour of the leading edge cells during embryonic dorsal closure.

    Rho1 is necessary for actin cable formation, but not cellular extenion formation during wound healing in the embryo.

    Rho1 is required for neuroblast proliferation but not for neuronal survival in the mushroom body.

    Together with Pkn, Rho1 mediates a pathway for cell shape changes in dorsal closure that is independent of the Rac GTPase-mediated Jun amino (N)-terminal kinase (JNK) cascade.

    Rho1 is required for many early events, both maternally and zygotically. Rho1 does not interact with members of the JNK signalling pathway.

    pbl is linked through Rho1 activity to the reorganisation of the actin cytoskeleton.

    Rho1 is required for invagination of mesodermal and endodermal primordia during gastrulation and its activation is likely to be regulated by RhoGEF2.

    Complements S.cerevisiae RHO1 mutation.

    Rho1 has been cloned and sequenced. Rho1 is required for the generation of tissue polarity. Genetic interactions indicate that Rho1 has a role in signalling mediated by fz and dsh.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 108 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (36)
    Reported As
    Symbol Synonym
    AAF01186
    Dm Rho1
    Rho
    (Blackie et al., 2020, Thuma et al., 2018, Gilmour et al., 2017, Ly et al., 2017, Battistini and Tamagnone, 2016, Clavería and Torres, 2016, Matsubayashi and Millard, 2016, Munjal et al., 2015, Takeda et al., 2013, Weavers and Skaer, 2013, Murray et al., 2012, Kirilly et al., 2011, Muyskens and Guillemin, 2011, Szafer-Glusman et al., 2011, Ball et al., 2010, Blanchard et al., 2010, Goldbach et al., 2010, He et al., 2010, Uehara et al., 2010, Gupta et al., 2009, Homem and Peifer, 2009, Schotman et al., 2009, Larson et al., 2008, Ng, 2008, Warren-Paquin et al., 2008, Artabazon et al., 2007, Avet-Rochex et al., 2007, Dorsten et al., 2007, Gregory et al., 2007, Xu et al., 2007, Fehon, 2006, Homem et al., 2006, Hozumi et al., 2006, Kolesnikov, 2006, Stewart et al., 2006, Talbot and VanBerkum, 2006, Xu et al., 2006, Yang and Bashaw, 2006, Bejsovec, 2005, Emery et al., 2005, Grzeschik and Knust, 2005, McClatchey and Giovannini, 2005, Rosales-Nieves et al., 2005, Stewart and Ward, 2005, Stramer et al., 2005, Talbot and VanBerkum, 2005, Avet-Rochex et al., 2004, D'Avino et al., 2004, Finn and Fehon, 2004, Fox et al., 2004, Jefferson et al., 2004, Keung et al., 2004, Stramer et al., 2004, Fan et al., 2003, Jekely, 2003, Matani et al., 2003, Shilo, 2003, Speck et al., 2003, Baum, 2002, Bokel and Brown, 2002, Etienne-Manneville and Hall, 2002, Manning et al., 2002, Park et al., 2002, Strutt et al., 2002, Bashaw et al., 2001, Bashaw et al., 2001, Bee et al., 2001, Chen et al., 2001, Fritz and VanBerkum, 2001, Fritz and Vanberkum, 2001, Guichard et al., 2001, Jacinto and Martin, 2001, Jacinto et al., 2001, Johnson et al., 2001, Bloor and Kiehart, 2000, Chang and Ready, 2000, Chen et al., 2000, Luo, 2000, Seidensticker and Behrens, 2000, Frasch, 1999, Magie et al., 1999, Martin-Bermudo et al., 1999, Montell, 1999, Sutherland and Witke, 1999, Bloor and Kiehart, 1998, Crawford et al., 1998, Kerber et al., 1998, O'Leary et al., 1998, Meyer and Parkhurst, 1997, Meyer and Parkhurst, 1997)
    Rho1
    (Chougule et al., 2020, Dehapiot et al., 2020, Eritano et al., 2020, Nakamura et al., 2020, Popkova et al., 2020, Verboon et al., 2020, Bailles et al., 2019, Banerjee et al., 2019, Chen et al., 2019, Dent et al., 2019, Franco and Carmena, 2019, Gao et al., 2019, Garcia De Las Bayonas et al., 2019, Herrera-Perez and Kasza, 2019, Ho et al., 2019, Jiang et al., 2019, Li et al., 2019, Rodriguez-Fernandez et al., 2019, Sarpal et al., 2019, Silver et al., 2019, Singh et al., 2019, Steinhauer et al., 2019, Wang et al., 2019, Das et al., 2018, Hao et al., 2018, Lee et al., 2018, Mortensen et al., 2018, Qin et al., 2018, Richardson and Portela, 2018, Schmidt and Grosshans, 2018, Segal et al., 2018, Sotillos et al., 2018, Toret et al., 2018, Banisch et al., 2017, Couturier et al., 2017, Hernandez-Fleming et al., 2017, Nakamura et al., 2017, Park et al., 2017, Tran and Ten Hagen, 2017, Bielmeier et al., 2016, Das et al., 2016, Gene Disruption Project members, 2016-, Green et al., 2016, Kockel et al., 2016, Kuleesha et al., 2016, Levinson and Cagan, 2016, Ma et al., 2016, Minjarez et al., 2016, Ng et al., 2016, Rousso et al., 2016, Ruan et al., 2016, Sarov et al., 2016, Schimizzi et al., 2016, Takashima et al., 2016, Hanlon and Andrew, 2015, Morán et al., 2015, Myat et al., 2015, Ojelade et al., 2015, Ojelade et al., 2015, Petsakou et al., 2015, Rosa et al., 2015, Verboon and Parkhurst, 2015, Verboon et al., 2015, Vlachos et al., 2015, Abreu-Blanco et al., 2014, Bausek and Zeidler, 2014, Eliazer et al., 2014, Fernández et al., 2014, Gersten et al., 2014, Hain et al., 2014, Hsieh et al., 2014, Iordanou et al., 2014, Kitazawa et al., 2014, Nie et al., 2014, Rudrapatna et al., 2014, Sechi et al., 2014, Simões et al., 2014, Swope et al., 2014, Toret et al., 2014, Yashiro et al., 2014, Bonke et al., 2013, Carter, 2013, Das and Cagan, 2013, Das et al., 2013, Das et al., 2013, Fernandez-Gonzalez and Zallen, 2013, Greer et al., 2013, Guillot and Lecuit, 2013, Khoo et al., 2013, Melzer et al., 2013, Morais-de-Sá and Sunkel, 2013, Neisch et al., 2013, Parkhurst, 2013.10.2, Peters and Rogers, 2013, Pirraglia et al., 2013, Rousso et al., 2013, Saravanan et al., 2013, Schertel et al., 2013, Sen et al., 2013, Sen et al., 2013, Takeda et al., 2013, Terriente-Felix et al., 2013, Vaque et al., 2013, Vaqué et al., 2013, Webber et al., 2013, Yin et al., 2013, Baek et al., 2012, Chountala et al., 2012, Felix et al., 2012, Giansanti and Fuller, 2012, Howell et al., 2012, Iyer et al., 2012, Jeon et al., 2012, Jeong et al., 2012, Morris et al., 2012, Axelsson et al., 2011, Brumby et al., 2011, Degennaro et al., 2011, Fauvarque and Williams, 2011, Johnson et al., 2011, Lee et al., 2011, Makhijani et al., 2011, Song and Giniger, 2011, Sun et al., 2011, Szafer-Glusman et al., 2011, Takemura and Adachi-Yamada, 2011, Tanaka et al., 2011, Taniguchi et al., 2011, Xu et al., 2011, Asensio et al., 2010, Baek et al., 2010, Ball et al., 2010, Blanchard et al., 2010, Campos et al., 2010, Elsaesser et al., 2010, Hughes et al., 2010, Kadandale et al., 2010, Neisch et al., 2010, Pataki et al., 2010, Renault et al., 2010, Sato et al., 2010, Sekyrova et al., 2010, Simões et al., 2010, von Hilchen et al., 2010, Warner, 2010.9.15, Yu et al., 2010, Zimmerman et al., 2010, Aleksic et al., 2009, Dworkin et al., 2009, Frank et al., 2009, Liu et al., 2009, Massarwa et al., 2009, Mirouse et al., 2009, Patch et al., 2009, Snee and Macdonald, 2009, Tan et al., 2009, van Impel et al., 2009, Warner and Longmore, 2009, Warner and Longmore, 2009, Webb et al., 2009, Widmann and Dahmann, 2009, Yan et al., 2009, Alves-Silva et al., 2008, Artabazon et al., 2008, Benjamin et al., 2008, Hickson and O'Farrell, 2008, Homem and Peifer, 2008, Johnson et al., 2008, Koelsch and Leptin, 2008, Larson et al., 2008, Matusek et al., 2008, Morgan and Kalm, 2008, Patch et al., 2008, Qin et al., 2008, Sweeney et al., 2008, Xu et al., 2008, Xu et al., 2008, Zhou et al., 2008, Bakal et al., 2007, Bakal et al., 2007, Beltran et al., 2007, Betson and Settleman, 2007, Cordero et al., 2007, Fox and Peifer, 2007, Luo, 2007.3.23, Minidorff et al., 2007, Minidorff et al., 2007, Mulinari et al., 2007, Neisch et al., 2007, Quinones-Coello, 2007, Taniguchi et al., 2007, Wang and Riechmann, 2007, Wenzl and Großhans, 2007, Williams et al., 2007, Zeitlinger et al., 2007, Zhang et al., 2007, Brodu and Casanova, 2006, Dean and Spudich, 2006, Lovegrove et al., 2006, Polaski et al., 2006, Rothenfluh et al., 2006, Simoes et al., 2006, Vidal et al., 2006, Vig et al., 2006, Avet-Rochex et al., 2005, Chen et al., 2005, Fox et al., 2005, He et al., 2005, Padash Barmchi et al., 2005, Sommer et al., 2005, Stramer et al., 2005, Paladi and Tepass, 2004, Rogers et al., 2003)
    RhoA
    (Greig and Bulgakova, 2020, Martin, 2020, Miwa et al., 2020, Thuveson et al., 2019, Verma and Maresca, 2019, Verma and Maresca, 2019, Dunn et al., 2018, Katanaev et al., 2018, Spinner et al., 2018, Carvajal-Gonzalez et al., 2016, Gombos et al., 2015, Hosono et al., 2015, Rodal et al., 2015, Sterne et al., 2015, Sundaram, 2015, Lammel et al., 2014, Pichaud, 2014, Puram and Bonni, 2013, Saravanan et al., 2013, Vaqué et al., 2013, Warrington et al., 2013, Zuo et al., 2013, Cook et al., 2012, Bassi et al., 2011, Ben El Kadhi et al., 2011, Hermle et al., 2011, Matsubara et al., 2011, Mirkovic et al., 2011, Nahm and Lee, 2011, Szafer-Glusman et al., 2011, Blanchard et al., 2010, Cao et al., 2010, Colinet et al., 2010, Nahm et al., 2010, Pataki et al., 2010, Zeng et al., 2010, Schotman et al., 2009, Albertson et al., 2008, Cao et al., 2008, Carreno et al., 2008, Gregory et al., 2008, Llense and Martín-Blanco, 2008, Matusek et al., 2008, Morgan and Kalm, 2008, Rodriguez-Diaz et al., 2008, Bastock and Strutt, 2007, Bidla et al., 2007, Chung et al., 2007, Colinet et al., 2007, Corrigall et al., 2007, Franke et al., 2007, Gregory et al., 2007, Impel et al., 2007, Montrasio et al., 2007, Muñoz-Descalzo et al., 2007, Roubinet et al., 2007, Taniguchi et al., 2007, Tolwinski, 2007, Tyler et al., 2007, Fox and Kalm, 2006, Kerkhoff, 2006, Matusek et al., 2006, McBride et al., 2006, Minin et al., 2006, Mirkovic and Mlodzik, 2006, Nahm et al., 2006, Ruggiero et al., 2006, Sanny et al., 2006, Srahna et al., 2006, D'Avino et al., 2005, Govek et al., 2005, Hu et al., 2005, Matusek et al., 2005, Ng and Luo, 2005, Piekny et al., 2005, Wilson et al., 2005, Zhu et al., 2005, Balasubramanian et al., 2004, Halsell et al., 2004, Ishimaru et al., 2004, Polesello and Payre, 2004, Shandala et al., 2004, Starz-Gaiano and Montell, 2004, Wilk et al., 2004, Bayer et al., 2003, Echard and O'Farrell, 2003, Gao and Bogert, 2003, Ghabrial et al., 2003, Goda and Davis, 2003, Jacinto and Baum, 2003, Lee et al., 2003, Pasterkamp and Kolodkin, 2003, Schumacher et al., 2003, Solnica-Krezel and Eaton, 2003, Strutt, 2003, Strutt and Strutt, 2003, Strutt and Strutt, 2003, Tan et al., 2003, Adler, 2002, Aigaki et al., 2002, Bloor and Kiehart, 2002, Castellani and Rougon, 2002, Harden et al., 2002, Kolodziej and Lee, 2002, Lee and Kolodziej, 2002, Mlodzik, 2002, Patel and Van Vactor, 2002, Strutt et al., 2002, Tree et al., 2002, von Kalm et al., 2002, Wedlich, 2002, Wood et al., 2002, Adler and Lee, 2001, Billuart et al., 2001, Billuart et al., 2001, Bloor and Kiehart, 2001, Bloor and Kiehart, 2001, Cooper and Strutt, 2001, Das and Mlodzik, 2001, Hu et al., 2001, Johnstone and Lasko, 2001, Pilch et al., 2001, Somers et al., 2001, Strutt, 2001, Uchida et al., 2001, Whitford and Ghosh, 2001, Wiemann et al., 2001, Winter et al., 2001, Winter et al., 2001, Acebes and Ferrus, 2000, Fanto, 2000, Fanto et al., 2000, Halsell and Kiehart, 2000, Halsell et al., 2000, Lee et al., 2000, Mlodzik, 2000, Newsome et al., 2000, O'Keefe et al., 2000, Pazman et al., 2000, Peifer and Tepass, 2000, Sotillos and Campuzano, 2000, Asha et al., 1999, Halsell and Kiehart, 1999, Harden et al., 1999, Harden et al., 1999, Kollmar, 1999, Lee et al., 1999, Mlodzik, 1999, Paricio et al., 1999, Rangarajan et al., 1999, Reifegerste and Moses, 1999, Sawamoto et al., 1999, Sawamoto et al., 1999, Sutherland and Witke, 1999, Thomas and Wassarman, 1999, Axelrod et al., 1998, Boutros et al., 1998, Dale, 1998, Goberdhan and Wilson, 1998, Shulman et al., 1998, Tree and Gubb, 1998, Eaton, 1997, Martin-Blanco, 1997, Strutt et al., 1997, Strutt et al., 1997, Van Aelst and D'Souza-Schorey, 1997)
    l(2)52Fa
    Secondary FlyBase IDs
    • FBgn0014021
    • FBgn0024874
    • FBgn0024880
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (766)