Gene Dmel\Rho1

General Information
Symbol	Dmel\Rho1	Species	D. melanogaster
Name	Rho1	Annotation symbol	CG8416
Feature type	protein_coding_gene	FlyBase ID	FBgn0014020
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	52E5-52E5	Sequence location	2R:11,990,191..11,994,734 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Rho1 (CG8416, FBgn0014020). It has the cytological map location 52E5. Its sequence location is 2R:11990191..11994734. Its molecular function is described as: GTPase activity; protein binding; kinase binding; GTP binding. It is involved in the biological processes described with 42 unique terms, many of which group under: anatomical structure development; cell motility; actin filament-based process; open tracheal system development; organelle organization and biogenesis; cell communication; establishment of planar polarity; embryonic development via the syncytial blastoderm; imaginal disc-derived wing hair organization and biogenesis; transport; actin cable formation; transmembrane receptor protein tyrosine kinase signaling pathway; small GTPase mediated signal transduction; cell division; JNK cascade; wound healing; ommatidial rotation; Wnt receptor signaling pathway; epithelial cell differentiation; regulation of neurogenesis. 57 alleles are reported. The phenotypes of these alleles are annotated with 56 unique terms, many of which group under: organ system; peripheral nervous system; nervous system; embryonic/larval tracheal system; spiracle; extended germ band embryo; primordium; portion of tissue; embryonic nervous system; embryonic hindgut. It has 7 annotated transcripts and 7 annotated polypeptides.
External Summaries

Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 52E5-52E5 Limits computationally determined from genome sequence between P{PZ}l(2)05070⁰⁵⁰⁷⁰&P{EP}CG8414^EP525 and P{EP}spin^EP645&P{lacW}spin^k09905
Experimentally Determined Cytological Location
Cytogenetic map Notes References 52E-52E (Magie, 1999.8.17) 52E-52E (determined by in situ hybridisation) (Magie et al., 1999) 52E5-52E6 (determined by in situ hybridisation) (Spradling et al., 1999) 52E3-52E6 (determined by in situ hybridisation) (Settleman and Hariharan, 1997.8.8) 52E2-52E9 (determined by in situ hybridisation) (Sasamura et al., 1997) 52E3-52E6 (determined by in situ hybridisation) (Strutt et al., 1997) 52D-52D (determined by in situ hybridisation) (Sasamura, 1995) 3C-3C (determined by in situ hybridisation) (Hariharan et al., 1995) 52E5-52E6 (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Rho1 for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rho1-RA FBtr0087211 NM_057750 1195 192 Rho1-RB FBtr0087212 NM_134308 2029 192 Rho1-RC FBtr0087213 NM_134309 1798 192 Rho1-RD FBtr0087214 NM_166139 1983 192 Rho1-RE FBtr0087215 NM_206129 763 192 Rho1-RF FBtr0087216 NM_206128 1972 192 Rho1-RG FBtr0087217 NM_206127 1975 192
Additional Transcript Data & Comments
Reported size (kB)	3.7, 3.1, 2.1 (northern blot); 1.3 (unknown) (Magie et al., 1999) 1.467 (longest cDNA) (Hariharan et al., 1995)
Comments	Rho1 cDNAs fall into six different classes of transcripts, all encoding the same 192 amino acid ORF. The size differences result from alternate splicing in the 5\\' and 3\\' UTRs. Four transcript sizes of 1.3, 2.1, 3.1 and 3.7 kb are detected in Northern blots; each size band probably represents multiple forms. (Magie et al., 1999)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Rho1-PA FBpp0086353 21.7 192 7.06 NP_477098 AAM70944 Rho1-PB FBpp0086354 21.7 192 7.06 NP_599135 AAF58066 Rho1-PC FBpp0086355 21.7 192 7.06 NP_599136 AAM70945 Rho1-PD FBpp0086356 21.7 192 7.06 NP_725524 AAM70946 Rho1-PE FBpp0089128 21.7 192 7.06 NP_995851 AAS64842 Rho1-PF FBpp0089129 21.7 192 7.06 NP_995850 AAS64843 Rho1-PG FBpp0089130 21.7 192 7.06 NP_995849 AAS64844
Additional Polypeptide Data & Comments
Reported size (kD)	192 (aa) (Hariharan et al., 1995)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0014020
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ras GTPase (IPR001806) Ras small GTPase, Rho type (IPR003578) Small GTP-binding protein (IPR005225) Ras (IPR013753)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202784 AA391196 AA694683 AA695628 AA735703 AA802622 AA802783 AA816258 AA817053 AA949454 AA952270 AC004248 AE003808 AAF58066 AE003808 AAM70944 AE003808 AAM70945 AE003808 AAM70946 AE003808 AAS64842 AE003808 AAS64843 AE003808 AAS64844 AF177871 AAF01183 AF177872 AAF01184 AF177873 AAF01185 AF177874 AAF01186 AI239017 AI257703 AI294261 AI389565 AI513351 AW940792 AW941520 AY119536 AAM50190 BF495178 BG636136 BI161597 BI161598 BI162525 BI162526 BI163292 BI164637 BI165104 BI165570 BI166028 BI169325 BI214262 BI227920 BI231031 BI234630 BI238440 BI238583 BI241605 BI356759 BI361565 BI361584 BI362069 BI362544 BI362780 BI366426 BI367070 BI367429 BI367510 BI368645 BI368936 BI370112 BI374913 BI376156 BI481582 BI482701 BI487064 BI564367 BI569182 BI569183 BI575188 BI576690 BI579900 BI589315 BI590559 BI604686 BI605229 BI605555 BI605882 BI606348 BI606784 BI606829 BI607872 BI609659 BI609807 BI610789 BI611133 BI614834 BI616115 BI616247 BI619407 BI621206 BI623475 BI624050 BI629139 BI629458 BI630392 BI630939 BI638865 BP548744 BP549286 BP549287 BP551502 BP551503 BP556255 BP556256 BP557975 BP557976 BP560111 BP560112 BT010085 AAQ22554 CA804415 CK657168 CO181427 CO181605 CO183371 CO187194 CO188444 CO265494 CO267587 CO275313 CO280485 CO281822 CO283057 CO286515 CO296814 CO299865 CO300386 CO302704 CO303880 CO304272 CO308692 CO319127 CO324269 CO338075 CS026563 CAI61748 CZ466213 CZ466317 CZ466328 CZ466342 CZ466702 CZ472750 CZ475601 CZ475602 CZ475782 EC060482 EC061692 EC064343 EC065171 EC073756 EC078037 EC078530 EC084306 EC086203 EC089155 EC200810 EC202676 EC203944 EC206460 EC210707 EC211085 EC213726 EC213941 EC213986 EC218092 EC222250 EC223206 EC234397 EC234901 EC245644 EC262511 L38311 RE01068 RE01816 RH01339

UniProtKB/Swiss-Prot	P48148
UniProtKB/TrEMBL

Maps to	DS03910 (FlyBase, 1992-, Halsell et al., 2000)
Does NOT map to
Identified with	GH20776 LD03419 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References deletion Rho1[1B] 2R:11,991,280..11,993,556 comment=Mutation is a deletion. Upstream breakpoint is in the first intron after amino acid 52. The distal breakpoint is unknown but extends past the coding region. evidence=experimental (Magie and Parkhurst, 2005) point mutation Rho1[J3.8]