FB2025_04 , released October 2, 2025
Gene: Dmel\Agxt
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General Information
Symbol
Dmel\Agxt
Species
D. melanogaster
Name
Alanine-glyoxylate aminotransferase
Annotation Symbol
CG3926
Feature Type
protein_coding_gene
FlyBase ID
FBgn0014031
Gene Model Status
Current
Stock Availability
6 publicly available
Enzyme Name (EC)
alanine--glyoxylate transaminase (2.6.1.44)
serine--pyruvate transaminase (2.6.1.51)
Gene Summary
Alanine-glyoxylate aminotransferase (Agxt) encodes an enzyme involved in glyoxylate catabolism. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Spat, Serine pyruvate aminotransferase, spa

Key Links
Genomic Location
Cytogenetic map
6B1-6B1
Sequence location
Recombination map
1-17
RefSeq locus
NC_004354 REGION:6528948..6530726
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables alanine-glyoxylate transaminase activity
inferred from direct assay
(Han and Li, 2002)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables alanine-glyoxylate transaminase activity
inferred from biological aspect of ancestor with PANTHER:PTN000475663
(GO Reference Genome Project, 2011-)
enables L-serine-pyruvate transaminase activity
inferred from biological aspect of ancestor with PANTHER:PTN000475663
(GO Reference Genome Project, 2011-)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in glyoxylate catabolic process
inferred from direct assay
(Han and Li, 2002)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in glycine biosynthetic process, by transamination of glyoxylate
inferred from biological aspect of ancestor with PANTHER:PTN000475663
(GO Reference Genome Project, 2011-)
inferred by curator from GO:0008453
(Han and Li, 2002)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in peroxisome
inferred from biological aspect of ancestor with PANTHER:PTN000475663
(GO Reference Genome Project, 2011-)
located_in peroxisome
inferred from sequence or structural similarity with UniProtKB:P21549
(Faust et al., 2012)
inferred from sequence model
(Faust et al., 2012)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. (Q9W3Z3)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
L-serine-pyruvate transaminase activity
L-serine + pyruvate = 3-hydroxypyruvate + L-alanine (2.6.1.51)
RHEA 22852: alanine-glyoxylate transaminase activity
glyoxylate + L-alanine = glycine + pyruvate (2.6.1.44)
RHEA 24248:
Summaries
Gene Snapshot
Alanine-glyoxylate aminotransferase (Agxt) encodes an enzyme involved in glyoxylate catabolism. [Date last reviewed: 2019-09-19]
Gene Group (FlyBase)
TRANSAMINASES -
Transaminases catalyze the interconversion of amino acids and oxoacids by transfer of amino groups.
Protein Function (UniProtKB)
Catalyzes the pyridoxal 5'-phosphate-dependent transamination of alanine with glyoxylate as an amino group acceptor (PubMed:12220660). Can also catalyze, although with much less efficiency, the transamination of amino-butyrate, phenylalanine and serine with glyoxylate or pyruvate as an amino group acceptor (PubMed:12220660). Does not catalyze the transamination of both 3-hydroxykynurenine and L-kynurenine (PubMed:12220660). May play a role in the detoxification of glyoxylate, a toxic plant metabolite from the fly diet (PubMed:12220660).
(UniProt, Q9W3Z3)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Agxt for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9W3Z3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.51

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Agxt-RA
FBtr0070913
NM_078507
1410
394
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Agxt-PA
FBpp0070875
43.8
394
7.78
Q9W3Z3
NP_511062
AAF46168
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q9W3Z3)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Agxt using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.69

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Agxt in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Agxt
Transgenic constructs containing regulatory region of Agxt
Aberrations (Deficiencies and Duplications) ( 0 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\AGXT
14 of 14
Yes
Yes
Hsap\PSAT1
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Agxt
14 of 14
Yes
Yes
Rnor\Psat1
1 of 14
No
No
Mus musculus (laboratory mouse) (2)
Mmus\Agxt
13 of 14
Yes
Yes
Mmus\Psat1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (2)
Xtro\agxt
7 of 13
Yes
Yes
Xtro\ghrh
1 of 13
No
Yes
Danio rerio (Zebrafish) (2)
Drer\agxta
14 of 14
Yes
Yes
Drer\agxtb
14 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\agxt-1
14 of 14
Yes
Yes
Cele\F26H9.5
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP003490
12 of 12
Yes
Yes
Agam\AgaP_AGAP010387
6 of 12
No
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\AGT
10 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\AGX1
10 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Agxt. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
Psat
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
AgxtTH02225
model of  primary hyperoxaluria type 1
CEA
(Yang et al., 2018)
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
AGXT; alanine--glyoxylate aminotransferase
model of  primary hyperoxaluria type 1
IEA
(FlyBase, 2019-)
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
AGXT; alanine--glyoxylate aminotransferase
14 of 14
604285
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Interaction Browsers
Summary of Genetic Interactions
Interaction Browsers
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Homodimer.
(UniProt, Q9W3Z3 )
Linkouts
DroID - A comprehensive database of gene and protein interactions.
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyBase
External Links
FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
External Data
Linkouts
KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Class of Gene
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map
1-17
Cytogenetic map
6B1-6B1
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
6B1-6B1
Limits computationally determined from genome sequence between P{EP}EP1402&P{EP}CG15893EP1634 and P{EP}Ctr1AEP1364&P{EP}EP1445
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
6A-6B
(Fried, 1994.11.17)
Experimentally Determined Recombination Data
Location

1-17

(FlyBase, 2016)

1-18.2

(Comeron et al., 2012)

1-

(Fried, 1994.11.17)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (6)
Genomic Clones (17)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (186)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Cell Line Information
Publicly Available Cell Lines
 
    Other Stable Cell Lines
     
      Other Comments

      Identified during molecular analysis of the RpL7A genomic region.

      (Armes and Fried, 1995)
      Relationship to Other Genes
      Source for database merge of
      Additional comments
      Nomenclature History
      Source for database identify of

      Source for identity of: Agxt Spat

      (FlyBase, 2009-)
      Nomenclature comments

      Changed symbol/name from 'Spat/Serine pyruvate aminotransferase' to 'Agxt/Alanine-glyoxylate aminotransferase' to reflect the major activity of the encoded protein (see FBrf0151702) and also to follow the nomenclature used for orthologs and in another paper characterizing the fly gene (FBrf0239345).

      (FlyBase, 2009-)
      Etymology
      Synonyms and Secondary IDs (12)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      Study result (0)
      Result
      Result Type
      Title
      External Crossreferences and Linkouts ( 53 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      FlyMine - An integrated database for Drosophila genomics
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL - MARRVEL (model organism gene)
      Linkouts
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
      References (57)