Gene model reviewed during 5.52
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vm32E using the Feature Mapper tool.
Vm32E is expressed by the main-body follicle cells but is missing frm the anterior-most and posterior-most cells.
Vm32E is detected in the follicle cells of stage 10 egg chambers. Columnar main body follicle cells are intensely stained, while anterior- and posterior-most follicle cells are unstained. No staining is detected after stage 11. A Ecol\lacZ reporter construct (1069 bp of upstream Vm32E sequence and the first 232 bp of transcribed sequence: Vm32E-1069:+232.T:Ecol\lacZ) shows a comparable pattern of expression.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Vm32E in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Vm32E upstream sequences between -135bp and -113bp are essential for expression in the ventral columnar follicle cells, while expression in the dorsal follicular cells requires upstream sequences between -348bp and -254bp and also between -118bp and -39bp. the region between -253bp and -119bp contains a negative element that represses expression in the anterior centripetal cells.