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Gene Dmel\barr

General Information
Symbol	Dmel\barr	Species	D. melanogaster
Name	barren	Annotation symbol	CG10726
Feature type	protein_coding_gene	FlyBase ID	FBgn0014127
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	CAP-H, barren, 38B.3
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	38B1-38B2	Sequence location	2L:20,058,163..20,060,975 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene barren is referred to in FlyBase by the symbol Dmel\barr (CG10726, FBgn0014127). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DNA topoisomerase (ATP-hydrolyzing) activator activity. There is experimental evidence that it is involved in the biological process: morphogenesis of an epithelium; mitotic chromosome condensation; Malpighian tubule morphogenesis; tricarboxylic acid cycle; mitotic sister chromatid separation; mitotic spindle organization; head involution; ectodermal digestive tract morphogenesis; open tracheal system development; neurogenesis. 22 alleles are reported. The phenotypes of these alleles are annotated with: embryonic head; neuron; embryonic/larval trachea; mitotic cell cycle; embryonic Malpighian tubule; midgut constriction; embryonic epidermis; multidendritic neuron; presumptive embryonic/larval peripheral nervous system; peripheral nervous system. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Condensin complex subunit 2/barren. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1627999_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of barr. Gene sequence location is 2L:20058163..20060975.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 38B1-38B2   Limits computationally determined from genome sequence between P{lacW}l(2)k10239k10239 and P{lacW}barrk14014
Experimentally Determined Cytological Location
Cytogenetic map Notes References 38B2-38B4     (Bhat, 1996.10.11) 38B1-38B2   (determined by in situ hybridisation)   (Spradling et al., 1999) 38B1-38B2   (determined by in situ hybridisation)   (Bhat et al., 1996) 38A-38A   (determined by in situ hybridisation)   (Kania et al., 1995) 38A-38A   (determined by in situ hybridisation)   (Bhat and Bellen, 1995) 38A-38A   38B1--2   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\barr for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.39
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) barr-RA FBtr0081348  NM_057758   2578   735 barr-RB FBtr0081349  NM_165317   2682   735
Additional Transcript Data & Comments
Reported size (kB)	2.6 (northern blot) (Bhat et al., 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein barr-PA   FBpp0080880   82.8   735   5.26     NP_477106   AAF53866 barr-PB   FBpp0080881   82.8   735   5.26     NP_724240   AAN11061
Additional Polypeptide Data & Comments
Reported size (kDa)	736 (aa); 97 (kD observed); 81 (kD predicted) (Bhat et al., 1996)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Condensin complex subunit 2/barren (IPR022816)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF53866   AE014134 AAN11061   AC004364   AQ034033   BF500398   BG633918   BI172723   BI232108   BI240689   BI363840   BI365411   BI371143   BT011446   BT011474   CL705894   CO186750   CO265293   CO265295   CO265297   CO265299   CO266932   CO273472   CO312223   CO312257   CO313209   CO329251   CO343628   EC078800   EC087663   EC198037   EC200360   EC210490   EC217743   EC225215   EC259595   EV576054   FE863520   FE863521   FE863649   FE863674   FE863675   FE863724   FE863780   FE863781   FE863837   GH738838   GH739167   GH740232   GH740687   GH744136   GH745866   GH761497   GH769471   GH769584   U74488 AAB40125
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VIP9 P91663
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) embryonic central brain glial cell   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) procephalic neuroblast   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
	barr is expressed in the embryo in a pattern similar to that of other genes necessary for cell cycle progression. barr transcripts are detected at all stages with northern blots with a peak between 6 and 12 hours of embryogenesis. (Bhat et al., 1996)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early   organism | ubiquitous   (Bhat et al., 1996)
Additional Descriptive Data
	The subcellular distribution of barr protein was characterized during mitosis 14. During interphase, barr protein is diffusely distributed in the cytoplasm with little or none in the nucleus. As chromosomes condense, barr protein is associated with DNA staining. barr protein is associated with chromatin throughout prophase, metaphase and anaphase but begins to disappear in late telophase. (Bhat et al., 1996)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
condensed chromosome inferred from direct assay (Bhat et al., 1996)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    392.375 Larval Midgut    28.9 Larval Hindgut    44.7 Larval Malpighian Tubules    40.4 Larval Fat Body    109.4 Larval Salivary Gland    35.3 Larval Trachea    92.15 Larval Carcass    48.85 Adult Head    48.6 Adult Eye    31.875 Adult Brain    54.5 Adult Thoracic-Abdominal Ganglion    55.3 Adult Crop    71.2 Adult Midgut    45.7 Adult Hindgut    60.5 Adult Malpighian Tubules    52 Adult Fat Body    35.7 Adult Salivary Gland    58.6 Adult Heart    27.225 Adult VirginFemale Spermatheca    45.1 Adult InseminatedFemale Spermatheca    45.2 Adult Ovary    545.3 Adult Testis    348.3 Adult Male Accessory Gland    53.5 Adult Carcass    45.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    392.375 Larval Midgut    28.9 Larval Hindgut    44.7 Larval Malpighian Tubules    40.4 Larval Fat Body    109.4 Larval Salivary Gland    35.3 Larval Trachea    92.15 Larval Carcass    48.85 Adult Head    48.6 Adult Eye    31.875 Adult Brain    54.5 Adult Thoracic-Abdominal Ganglion    55.3 Adult Crop    71.2 Adult Midgut    45.7 Adult Hindgut    60.5 Adult Malpighian Tubules    52 Adult Fat Body    35.7 Adult Salivary Gland    58.6 Adult Heart    27.225 Adult VirginFemale Spermatheca    45.1 Adult InseminatedFemale Spermatheca    45.2 Adult Ovary  (545.3) Adult Testis    348.3 Adult Male Accessory Gland    53.5 Adult Carcass    45.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    392.375 Larval Midgut    28.9 Larval Hindgut    44.7 Larval Malpighian Tubules    40.4 Larval Fat Body    109.4 Larval Salivary Gland    35.3 Larval Trachea    92.15 Larval Carcass    48.85 Adult Head    48.6 Adult Eye    31.875 Adult Brain    54.5 Adult Thoracic-Abdominal Ganglion    55.3 Adult Crop    71.2 Adult Midgut    45.7 Adult Hindgut    60.5 Adult Malpighian Tubules    52 Adult Fat Body    35.7 Adult Salivary Gland    58.6 Adult Heart    27.225 Adult VirginFemale Spermatheca    45.1 Adult InseminatedFemale Spermatheca    45.2 Adult Ovary    545.3 Adult Testis    348.3 Adult Male Accessory Gland    53.5 Adult Carcass    45.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    392.375 Larval Midgut    28.9 Larval Hindgut    44.7 Larval Malpighian Tubules    40.4 Larval Fat Body    109.4 Larval Salivary Gland    35.3 Larval Trachea    92.15 Larval Carcass    48.85 Adult Head    48.6 Adult Eye    31.875 Adult Brain    54.5 Adult Thoracic-Abdominal Ganglion    55.3 Adult Crop    71.2 Adult Midgut    45.7 Adult Hindgut    60.5 Adult Malpighian Tubules    52 Adult Fat Body    35.7 Adult Salivary Gland    58.6 Adult Heart    27.225 Adult VirginFemale Spermatheca    45.1 Adult InseminatedFemale Spermatheca    45.2 Adult Ovary    545.3 Adult Testis    348.3 Adult Male Accessory Gland    53.5 Adult Carcass    45.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    392.375 Larval Midgut    28.9 Larval Hindgut    44.7 Larval Malpighian Tubules    40.4 Larval Fat Body    109.4 Larval Salivary Gland    35.3 Larval Trachea    92.15 Larval Carcass    48.85 Adult Head    48.6 Adult Eye    31.875 Adult Brain    54.5 Adult Thoracic-Abdominal Ganglion    55.3 Adult Crop    71.2 Adult Midgut    45.7 Adult Hindgut    60.5 Adult Malpighian Tubules    52 Adult Fat Body    35.7 Adult Salivary Gland    58.6 Adult Heart    27.225 Adult VirginFemale Spermatheca    45.1 Adult InseminatedFemale Spermatheca    45.2 Adult Ovary    545.3 Adult Testis    348.3 Adult Male Accessory Gland    53.5 Adult Carcass    45.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    392.375 Larval Midgut    28.9 Larval Hindgut    44.7 Larval Malpighian Tubules    40.4 Larval Fat Body    109.4 Larval Salivary Gland    35.3 Larval Trachea    92.15 Larval Carcass    48.85 Adult Head    48.6 Adult Eye    31.875 Adult Brain    54.5 Adult Thoracic-Abdominal Ganglion    55.3 Adult Crop    71.2 Adult Midgut    45.7 Adult Hindgut    60.5 Adult Malpighian Tubules    52 Adult Fat Body    35.7 Adult Salivary Gland    58.6 Adult Heart    27.225 Adult VirginFemale Spermatheca    45.1 Adult InseminatedFemale Spermatheca    45.2 Adult Ovary  545.3 Adult Testis    348.3 Adult Male Accessory Gland    53.5 Adult Carcass    45.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    392.375 Larval Midgut    28.9 Larval Hindgut    44.7 Larval Malpighian Tubules    40.4 Larval Fat Body    109.4 Larval Salivary Gland    35.3 Larval Trachea    92.15 Larval Carcass    48.85 Adult Head    48.6 Adult Eye    31.875 Adult Brain    54.5 Adult Thoracic-Abdominal Ganglion    55.3 Adult Crop    71.2 Adult Midgut    45.7 Adult Hindgut    60.5 Adult Malpighian Tubules    52 Adult Fat Body    35.7 Adult Salivary Gland    58.6 Adult Heart    27.225 Adult VirginFemale Spermatheca    45.1 Adult InseminatedFemale Spermatheca    45.2 Adult Ovary    545.3 Adult Testis    348.3 Adult Male Accessory Gland    53.5 Adult Carcass    45.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    392.375 Larval Midgut    28.9 Larval Hindgut    44.7 Larval Malpighian Tubules    40.4 Larval Fat Body    109.4 Larval Salivary Gland    35.3 Larval Trachea    92.15 Larval Carcass    48.85 Adult Head    48.6 Adult Eye    31.875 Adult Brain    54.5 Adult Thoracic-Abdominal Ganglion    55.3 Adult Crop    71.2 Adult Midgut    45.7 Adult Hindgut    60.5 Adult Malpighian Tubules    52 Adult Fat Body    35.7 Adult Salivary Gland    58.6 Adult Heart    27.225 Adult VirginFemale Spermatheca    45.1 Adult InseminatedFemale Spermatheca    45.2 Adult Ovary    545.3 Adult Testis    348.3 Adult Male Accessory Gland    53.5 Adult Carcass    45.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    48.6   NA  Eye    31.875   NA  Brain    54.5   392.375  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    55.3   NA  Crop    71.2   28.9  Midgut    45.7   44.7  Hindgut    60.5   40.4  Malpighian Tubules    no informative data   109.4  Fat Body    no informative data   no informative data  Salivary Gland    58.6   NA  Heart    27.225   92.15  Trachea    NA   NA  VirginFemale Spermatheca    45.1   NA  InseminatedFemale Spermatheca    45.2   NA  Ovary    545.3   NA  Testis    348.3   NA  Male Accessory Gland    53.5   48.85  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    37 central nervous system, larvae L3    21 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    8 fat body, pupae P8    6 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    49 ovary, mated 4-day female    39 testis, mated 4-day male    29 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (37) central nervous system, larvae L3    21 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    8 fat body, pupae P8    6 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female  (49) ovary, mated 4-day female  (39) testis, mated 4-day male  (29) accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    37 central nervous system, larvae L3    21 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    8 fat body, pupae P8    6 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    49 ovary, mated 4-day female    39 testis, mated 4-day male    29 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    37 central nervous system, larvae L3    21 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    8 fat body, pupae P8    6 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    49 ovary, mated 4-day female    39 testis, mated 4-day male    29 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    37 central nervous system, larvae L3    21 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    8 fat body, pupae P8    6 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    49 ovary, mated 4-day female    39 testis, mated 4-day male    29 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (37) central nervous system, larvae L3    21 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    8 fat body, pupae P8    6 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female  (49) ovary, mated 4-day female  (39) testis, mated 4-day male  29 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    37 central nervous system, larvae L3    21 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    8 fat body, pupae P8    6 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    49 ovary, mated 4-day female    39 testis, mated 4-day male    29 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    37 central nervous system, larvae L3    21 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    8 fat body, pupae P8    6 carcass, larvae L3 wandering    12 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    49 ovary, mated 4-day female    39 testis, mated 4-day male    29 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    37 embryo 04-06hr    77 embryo 06-08hr    77 embryo 08-10hr    51 embryo 10-12hr    23 embryo 12-14hr    17 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    3 embryo 20-22hr    2 embryo 22-24hr    3 larva L1    3 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    13 white prepupae 12hr    23 white prepupae 24hr    27 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (62) embryo 02-04hr  (37) embryo 04-06hr  (77) embryo 06-08hr  (77) embryo 08-10hr  (51) embryo 10-12hr    23 embryo 12-14hr    17 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    3 embryo 20-22hr    2 embryo 22-24hr    3 larva L1    3 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    13 white prepupae 12hr    23 white prepupae 24hr  (27) pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day  26 adult female 30day  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    37 embryo 04-06hr    77 embryo 06-08hr    77 embryo 08-10hr    51 embryo 10-12hr    23 embryo 12-14hr    17 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    3 embryo 20-22hr    2 embryo 22-24hr    3 larva L1    3 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    13 white prepupae 12hr    23 white prepupae 24hr    27 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    37 embryo 04-06hr    77 embryo 06-08hr    77 embryo 08-10hr    51 embryo 10-12hr    23 embryo 12-14hr    17 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    3 embryo 20-22hr    2 embryo 22-24hr    3 larva L1    3 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    13 white prepupae 12hr    23 white prepupae 24hr    27 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    26 adult female 30day    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    37 embryo 04-06hr    77 embryo 06-08hr    77 embryo 08-10hr    51 embryo 10-12hr    23 embryo 12-14hr    17 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    3 embryo 20-22hr    2 embryo 22-24hr    3 larva L1    3 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    13 white prepupae 12hr    23 white prepupae 24hr    27 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (62) embryo 02-04hr  (37) embryo 04-06hr  (77) embryo 06-08hr  (77) embryo 08-10hr  (51) embryo 10-12hr    23 embryo 12-14hr    17 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    3 embryo 20-22hr    2 embryo 22-24hr    3 larva L1    3 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    13 white prepupae 12hr    23 white prepupae 24hr  27 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day  26 adult female 30day  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    37 embryo 04-06hr    77 embryo 06-08hr    77 embryo 08-10hr    51 embryo 10-12hr    23 embryo 12-14hr    17 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    3 embryo 20-22hr    2 embryo 22-24hr    3 larva L1    3 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    13 white prepupae 12hr    23 white prepupae 24hr    27 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    62 embryo 02-04hr    37 embryo 04-06hr    77 embryo 06-08hr    77 embryo 08-10hr    51 embryo 10-12hr    23 embryo 12-14hr    17 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    3 embryo 20-22hr    2 embryo 22-24hr    3 larva L1    3 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    14 white prepupae new    13 white prepupae 12hr    23 white prepupae 24hr    27 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    16 adult male 30day    14 adult female 01day    10 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    14 embryonic Kc167    10 embryonic S1    24 embryonic S3    26 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    15 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    11 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    44 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    20 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    21 ovary fGS/OSS    32 ovary OSC    32 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    14 embryonic Kc167    10 embryonic S1    24 embryonic S3  26 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    15 wing disc ML-DmD16-c3  (30) wing disc ML-DmD21    11 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3  (44) eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    20 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    21 ovary fGS/OSS  (32) ovary OSC  (32) ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    14 embryonic Kc167    10 embryonic S1    24 embryonic S3    26 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    15 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    11 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    44 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    20 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    21 ovary fGS/OSS    32 ovary OSC    32 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    14 embryonic Kc167    10 embryonic S1    24 embryonic S3    26 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    15 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    11 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    44 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    20 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    21 ovary fGS/OSS    32 ovary OSC    32 ovary OSS    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    14 embryonic Kc167    10 embryonic S1    24 embryonic S3    26 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    15 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    11 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    44 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    20 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    21 ovary fGS/OSS    32 ovary OSC    32 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    14 embryonic Kc167    10 embryonic S1    24 embryonic S3  26 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    15 wing disc ML-DmD16-c3  30 wing disc ML-DmD21    11 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3  (44) eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    20 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    21 ovary fGS/OSS  32 ovary OSC  32 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    14 embryonic Kc167    10 embryonic S1    24 embryonic S3    26 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    15 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    11 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    44 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    20 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    21 ovary fGS/OSS    32 ovary OSC    32 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    25 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    14 embryonic Kc167    10 embryonic S1    24 embryonic S3    26 leg disc CME L1    19 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    15 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    11 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    44 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    20 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    21 ovary fGS/OSS    32 ovary OSC    32 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    22 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult  (27) Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3  (28) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    22 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    22 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    22 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    22 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult  27 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3  28 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    22 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    22 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    22 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • RE15383 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014127 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10726-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for barr Stages(s) 1-3 Stages(s) 4-6 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal barrGD12427 lethal | embryonic stage | recessive barrk00310 barrk04805 barrk04912 barrk13903 barrk14014 barrk14028 barrL305 lethal | pupal stage, with Scer\GAL4pnr-MD237 barrGD12427 lethal | recessive barrE51 barrk04912 barrk08103 barrk14014 viable barrEY01867 Sterility Allele fertile barrEY01867 Other Phenotypes Allele enhancer of variegation, with In(1)wm4h barrL305 enhancer of variegation | partially, with P{hsp26-pt-T}39C-72 barrL305 mitotic cell cycle defective barrdsRNA.cSa neuroanatomy defective | larval stage barrGD12427 non-enhancer of variegation, with P{hsp26-pt-T}118E-10 barrL305 suppressor of variegation | dominant, with In(1)wm4h barrL305 wild-type barr+tXa barrhs.PB Phenotype manifest in Allele chordotonal organ | lateral barrk00310 chromosome barrdsRNA.cYa embryonic/larval neuroblast | larval stage barrGD12427 embryonic/larval pharynx | embryonic stage barrk14014 embryonic/larval proventriculus | embryonic stage barrk14014 embryonic/larval trachea barrk14014 embryonic epidermis barrk14014 embryonic head barrk14014 embryonic Malpighian tubule barrk14014 ganglion mother cell | larval stage barrGD12427 midgut constriction barrk14014 mitosis & nuclear chromosome barrdsRNA.cSa mitotic cell cycle barrdsRNA.cYa multidendritic neuron barrk00310 neuron barrL305 barrk00310 barrk04805 barrk04912 barrk13903 barrk14014 barrk14028 peripheral nervous system barrL305 barrk00310 barrk04805 barrk04912 barrk13903 barrk14014 barrk14028 presumptive embryonic/larval peripheral nervous system barrk04805 barrk04912 barrk13903 barrk14014 barrk14028
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of barr Class Mutagen Stocks Known lesion barrL305 amorphic allele - genetic evidence P-element activity 2 Yes barrk14014 P-element activity 2 Yes barrEY01867 P-element activity 1 -- barrk00310 P-element activity 1 Yes barrk08103 P-element activity 1 -- barrbe408 P-element activity 0 Yes barrE51 Delta2-3 0 Yes barrk04805 P-element activity 0 Yes barrk04912 P-element activity 0 Yes barrk13903 P-element activity 0 Yes barrk14028 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of barr Class Mutagen Stocks Known lesion barrGD12427 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes barrGL00584 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes barrHMS00049 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes barr+tMa in vitro construct - genomic fragment 0 Yes barr+tXa in vitro construct - genomic fragment 0 Yes barrdsRNA.cSa in vitro construct - RNAi 0 Yes barrdsRNA.cYa in vitro construct - RNAi 0 -- barrhs.PB in vitro construct - regulatory fusion 0 Yes barrKK101679 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes barrNIG.8004R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes barrScer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)barrk04805 (Bhat et al., 1996) Df(2L)barrk04912 (Bhat et al., 1996) Df(2L)be408 (Butler et al., 2001) Df(2L)BSC258 (Christensen and Cook, 2007.3.22) Df(2L)BSC860 (Cook et al., 2009.11.25) Df(2L)E51 (Xu et al., 2001) Df(2L)Exel7077 (Ryder, 2004.4.26) Df(2L)pr2b (Butler et al., 2001) Df(2L)pr37 (Butler et al., 2001) Df(2L)pr40 (Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)pr49 (Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)pr1122 (Butler et al., 2001) Df(2L)pr8311 (Butler et al., 2001) Df(2L)pr9201 (Butler et al., 2001) Df(2L)pr9202 (Butler et al., 2001) Df(2L)pr9204 (Butler et al., 2001) Df(2L)pr11163 (Butler et al., 2001) Df(2L)pr-A14 (BDGP Project Members, 1994-1999, Kania et al., 1995) Df(2L)pr-A16 (Bhat et al., 1996) Df(2L)pr-A20 (Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)pr-M1 (Butler et al., 2001) Df(2L)TE38A-1 (Bhat et al., 1996) Df(2L)TW1 (Butler et al., 2001) Df(2L)TW9 (Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)TW65 (Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)TW84 (Liu et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999, Kania et al., 1995) Df(2L)TW150 (Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)TW161 (Spradling et al., 1999, Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)VA12 (Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)VA19 (Butler et al., 2001, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2L)DS5 (Butler et al., 2001) Df(2L)DS6 (Butler et al., 2001) Df(2L)DS9 (Butler et al., 2001) Df(2L)pr1 (Butler et al., 2001) Df(2L)pr7 (Butler et al., 2001) Df(2L)pr1123 (Butler et al., 2001) Df(2L)pr9133 (Butler et al., 2001) Df(2L)pr11164 (Butler et al., 2001) Df(2L)Sd37 (Butler et al., 2001) Df(2L)TW50 (Butler et al., 2001, BDGP Project Members, 1994-1999, Bhat et al., 1996) Df(2L)TW158 (Butler et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12427} NA P{KK101679} NA P{NIG.8004R} NA P{TRiP.GL00584} NA P{TRiP.HMS00049} NA P{UASp-barr.EGFP} NA heat-shock construct P{CaSpeR-hs-barr} NA characterization construct P{barr+tXa} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}barrk00310 No P{lacW}barrk04805 No P{lacW}barrk04912 No P{lacW}barrk08103 No P{lacW}barrk13903 No P{lacW}barrk14014 No P{lacW}barrk14028 No insertion of mobile activating element P{EPgy2}barrEY01867 NA miscellaneous insertions P{?'lacW}barrL305 NA
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
DNA topoisomerase (ATP-hydrolyzing) activator activity inferred from direct assay (Bhat et al., 1996)
Biological Process
CV term Evidence References
ectodermal digestive tract morphogenesis inferred from mutant phenotype (Liu et al., 1999) head involution inferred from mutant phenotype (Liu et al., 1999) Malpighian tubule morphogenesis inferred from mutant phenotype (Liu et al., 1999) mitotic chromosome condensation inferred from mutant phenotype (Goshima et al., 2007) mitotic sister chromatid separation inferred from mutant phenotype (Bhat et al., 1996) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) morphogenesis of an epithelium inferred from mutant phenotype (Liu et al., 1999) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) open tracheal system development inferred from mutant phenotype (Liu et al., 1999) tricarboxylic acid cycle inferred from direct assay (Chen et al., 2008)
Cellular Component
CV term Evidence References
condensed chromosome inferred from direct assay (Bhat et al., 1996)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mitosis inferred from sequence or structural similarity with EMBL:U90125 (FlyBase, 1992-) mitotic chromosome condensation inferred from sequence or structural similarity with EMBL:U90125 (FlyBase, 1992-) mitotic sister chromatid segregation inferred from sequence or structural similarity with EMBL:U90125 (FlyBase, 1992-) traceable author statement (Prokopenko et al., 2000) peripheral nervous system development traceable author statement (Prokopenko et al., 2000)
Cellular Component
CV term Evidence References
condensin complex inferred from sequence or structural similarity with EMBL:U90125 (FlyBase, 1992-) inferred from sequence or structural similarity with human NCAPH (Ono et al., 2003) non-traceable author statement (Gonzalez and Bornens, 2000, Haering and Nasmyth, 2003) traceable author statement (Prokopenko et al., 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions barr allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61258 DroID - A comprehensive database of gene and protein interactions. • FBgn0014127 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35287
Orthologs
OrthoDB Orthologs (54) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6Z0BR6 Dipteran inclusive ortholog search EOG6STSCR Insect inclusive ortholog search EOG6XKT36 Arthropod inclusive ortholog search EOG6G4F5M Metazoa inclusive ortholog search EOG647TMF
Orthologs in Drosophila Species (EOG6Z0BR6)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\barr     Drosophila simulans   Dsim\GD21719 Y   Drosophila sechellia   Dsec\GM16969 Y   Drosophila erecta   Dere\GG21594 Y   Drosophila yakuba   Dyak\GE12613 Y   Drosophila ananassae   Dana\GF15460 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA27888     Drosophila pseudoobscura pseudoobscura   Dpse\GA27889     Drosophila pseudoobscura pseudoobscura   Dpse\GA10526 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA27887     Drosophila pseudoobscura pseudoobscura   Dpse\GA23749     Drosophila persimilis   Dper\GL26677     Drosophila persimilis   Dper\GL26676 Y   Drosophila persimilis   Dper\GL26339     Drosophila persimilis   Dper\GL26679     Drosophila willistoni   Dwil\GK18686 Y   Drosophila virilis   Dvir\GJ17893 Y   Drosophila mojavensis   Dmoj\GI17284 Y   Drosophila grimshawi   Dgri\GH13243 Y
Orthologs in non-Drosophila Dipterans (EOG6STSCR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005962   Anopheles gambiae Malaria mosquito  Agam\AGAP011508   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ012273
Orthologs in non-Dipteran Insects (EOG6XKT36)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002088   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG015529   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18280   Atta cephalotes Leafcutter ant  Acep\ACEP19168   Camponotus floridanus Florida carpenter ant  Cflo\CFLO21625   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17248   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13621   Linepithema humile Argentine ant  Lhum\LH11650   Pogonomyrmex barbatus Red harvester ant  Pbar\PB19775   Pogonomyrmex barbatus Red harvester ant  Pbar\PB19780   Solenopsis invicta Red fire ant  Sinv\SINV21005   Acyrthosiphon pisum Pea aphid  Apim\ACYPI32992   Acyrthosiphon pisum Pea aphid  Apim\ACYPI007571   Acyrthosiphon pisum Pea aphid  Apim\ACYPI086255   Acyrthosiphon pisum Pea aphid  Apim\ACYPI38528   Acyrthosiphon pisum Pea aphid  Apim\ACYPI45724   Bombyx mori Silkmoth  Bmor\BGIBMGA011861   Pediculus humanus Human body louse  Phum\PHUM172700   Tribolium castaneum Red flour beetle  Tcas\TC003441   Tribolium castaneum Red flour beetle  Tcas\TC012137   Tribolium castaneum Red flour beetle  Tcas\TC012237
Orthologs in non-Insect Arthropods (EOG6G4F5M)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_225892   Ixodes scapularis Deer tick  Isca\ISCW002227
Orthologs in non-Arthropod Metazoa (EOG647TMF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024801gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023720gn   Danio rerio Zebrafish  Drer\ncaph   Xenopus tropicalis Western clawed frog  Xtro\ncaph   Gallus gallus Domestic chicken  Ggal\ENSGALG00000022365   Gallus gallus Domestic chicken  Ggal\ENSGALG00000024128   Mus musculus House mouse  Mmus\Ncaph   Rattus norvegicus Norway rat  Rnor\Ncaph   Homo sapiens Human  Hsap\NCAPH
Human Orthologs (1)
Gene OMIM HGNC Hsap\NCAPH
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21719 Drosophila sechellia Dsec\GM16969 Drosophila erecta Dere\GG21594 Drosophila yakuba Dyak\GE12613 Drosophila ananassae Dana\GF15460 Drosophila pseudoobscura pseudoobscura Dpse\GA10526 Drosophila persimilis Dper\GL26676 Drosophila willistoni Dwil\GK18686 Drosophila virilis Dvir\GJ17893 Drosophila mojavensis Dmoj\GI17284 Drosophila grimshawi Dgri\GH13243
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	4402 y1 w67c23; barrL305/CyO 20131 y1 w67c23; P{EPgy2}barrEY01867 11117 y1 w67c23; P{lacW}barrk14014/CyO 34068 y1 sc* v1; P{TRiP.HMS00049}attP2 36624 y1 sc* v1; P{TRiP.GL00584}attP40
Kyoto	107718 y1 w67c23; barrL305/CyO 102144 y1 w67c23 ; P{lacW}barrk00310 / CyO 102619 y1 w67c23 ; P{lacW}barrk08103 / CyO 111336 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}barrk14014 P{neoFRT}40A/CyO y+
VDRC	v26759 w1118; P{GD12427}v26759 v26760 w1118; P{GD12427}v26760
Genomic Clones ( 2 )
	BACR10H13 BACR22E16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 47 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE15383 RE48802
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT15273 AT29895 RE28125 RE37596 RE50702 RE57971
Other clones	11076.1 37042 59677 101315 101415 190137 214511.1 CK01.25D.G9 Dmel_dig_016212_0603_2071 EK022727 EK111101 EK142203 EK142206 EK142209 EK142212 EK147956 EK228219 EK228260 EK231901 EK292342 EP17441 RM07459 RM07460 RM07659 RM07759 RM07760 RM07859 RM07860 RM07960 RM08159 RP01414 RP01518 RP001015535 RP001016403 RP001033915 RP001034366 RP001039526 RP001040254 RP001052111
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0014127 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35287
Antibody Information
	polyclonal (Bhat et al., 1996, Pek and Kai, 2011)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in defects in chromosome condensation and chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) Inactivation of barr using RNA-mediated interference in S2 cells results in extensive defects in chromosome condensation and disrupts chromatid segregation. (Somma et al., 2003) barr is misexpressed in the PNS of da mutant embryos. (Hassan and Vaessin, 1997) Mutations in barr cause a failure of chromosomes to segregate correctly at anaphase, resulting in massive anaphase bridging. barr is not necessary for cell cycle progression out of mitosis. barr encodes a novel, conserved protein that localises throughout chromosomes during mitosis. barr protein associates with Top2 protein and affects its activity, this suggests barr protein functions in chromatid decatenation during the metaphase-anaphase transition. (Bhat et al., 1996) Weak alleles of barr cause sterile adults with rough eyes and few bristles that die 3-4 days after eclosion. Embryos exhibit loss of some external sensory and multidendritic neurons in the dorsal cluster and most of the chordotonal neurons in the lateral cluster. Severe alleles cause substantial loss of neurons, fasciculation defects and disruptions in the CNS longitudinal tracts. (Bhat and Bellen, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057758 NM_165317 RefSeq (Proteins) • NP_477106 NP_724240 DDBJ / EMBL / GenBank • AAB40125 AAF53866 AAN11061 AC004364 AQ034033 BI172723 BI363840 CL705894 U74488 Entrez Gene - A searchable database of RefSeq genes. • 35287 UniProtKB/TrEMBL • P91663 Q9VIP9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Condensin complex subunit 2/barren (IPR022816) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE15383
Linkouts
	BioGRID - A database of protein and genetic interactions • 61258 DroID - A comprehensive database of gene and protein interactions. • FBgn0014127 DRSC - Results from RNAi screens. • FBgn0014127 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014127 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10726-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0014127 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35287 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35287 modMine - Data generated by the modENCODE project. • FBgn0014127
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	38B.3 (McGill Drosophila Genome Project, 2001.9.25, Butler, 2001, Butler et al., 2001) barr (Christensen and Cook, 2007.3.22, Hueber et al., 2007, Georlette et al., 2007, Chen et al., 2008, Orian et al., 2007, Maezawa et al., 2009, Cook et al., 2009.11.25, Chen et al., 2008, Zhou et al., 2008, Pek and Kai, 2011, Japanese National Institute of Genetics, 2012.5.21) Barr (Pek and Kai, 2011) barren (Edwards et al., 2006) Barren (Oliveira et al., 2007, FBrf0192839, Ono et al., 2003) CAP-H (Savvidou and Heck, 2004, Oliveira et al., 2005, Somma et al., 2008, Savvidou et al., 2005) Cap-H (Herzog et al., 2008, Longworth et al., 2008) CG10726 (Goshima et al., 2007, Chen et al., 2008, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) dCap-H (Longworth et al., 2008)
Name Synonym	barren (Edwards et al., 2006, Bhat and Bellen, 1995, Maezawa et al., 2009, Chen et al., 2008) Barren (Savvidou and Heck, 2004, Oliveira et al., 2005, Goshima et al., 2007, Herzog et al., 2008, Oliveira et al., 2005, Somma et al., 2008, Orr et al., 2007, Chang et al., 2003, Coelho et al., 2003, Savvidou et al., 2005, Pimenta-Marques et al., 2008, Strukov et al., 2011)
Secondary FlyBase IDs
References ( 92 )
Generate a list of	All references relating to this gene ( 92 )
List References by type	Research paper  ( 46 ) Supplementary material  ( 8 ) Review  ( 12 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 7 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 4 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012 DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405] Pek and Kai, 2011, Curr. Biol. 21(1): 39--44 A role for vasa in regulating mitotic chromosome condensation in Drosophila. [FBrf0212718] Strukov et al., 2011, PLoS Biol. 9(1): e1000574 Evidence of Activity-Specific, Radial Organization of Mitotic Chromosomes in Drosophila. [FBrf0212852] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...